Restriction map for
pFN21AA0928
(Circular) (Six-base) MAPSORT of: pFN21AA0928.seq Check: 3828 from: 1 to: 10471
pFN21AA0928 10471 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3106 7489 3106
Size: 4383 6088
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8281 278
Size: 53 83 186 936 6745 2468
Fragments arranged by size:
6745 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 4745 7814 1937
Size: 804 2004 3069 4594
Fragments arranged by size:
4594 3069 2004 804
Acc65I G'GTAC_C
Cuts at: 7792 7792
Size: 10471
AclI AA'CG_TT
Cuts at: 1563 5468 8598 8971 1563
Size: 3905 3130 373 3063
Fragments arranged by size:
3905 3130 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2254 4410 4734 5331 5882 6788 8534 9918
Size: 622 2156 324 597 551 906 1746 1384
Cuts at: 9918 10186 1632
Size: 268 1917
Fragments arranged by size:
2156 1917 1746 1384 906 622 597 551
324 268
AfeI AGC'GCT
Cuts at: 5037 7112 5037
Size: 2075 8396
AflII C'TTAA_G
Cuts at: 820 1017 2693 820
Size: 197 1676 8598
Fragments arranged by size:
8598 1676 197
AflIII A'CryG_T
Cuts at: 1203 9370 10347 1203
Size: 8167 977 1327
Fragments arranged by size:
8167 1327 977
AgeI A'CCGG_T
Cuts at: 9302 9302
Size: 10471
AhdI GACnn_n'nnGTC
Cuts at: 5449 7210 9200 5449
Size: 1761 1990 6720
Fragments arranged by size:
6720 1990 1761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5486 6681 1184
Size: 4302 1195 4974
Fragments arranged by size:
4974 4302 1195
AlwNI CAG_nnn'CTG
Cuts at: 4619 4963 5101 6063 7595 8214 9786 4619
Size: 344 138 962 1532 619 1572 5304
Fragments arranged by size:
5304 1572 1532 962 619 344 138
ApaLI G'TGCA_C
Cuts at: 8528 9684 8528
Size: 1156 9315
ApoI r'AATT_y
Cuts at: 1493 2688 3032 3116 3301 3494 3524 4601 4688
Size: 1195 344 84 185 193 30 1077 87
Cuts at: 4688 4880 5665 6829 7006 7118 7601 7670 7780
Size: 192 785 1164 177 112 483 69 110
Cuts at: 7780 8006 8158 10267 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1195 1164 1077 785 483 344
226 193 192 185 177 152 112 110
87 84 69 30
AseI AT'TA_AT
Cuts at: 160 1032 5529 9025 160
Size: 872 4497 3496 1606
Fragments arranged by size:
4497 3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10471
AvaI C'yCGr_G
Cuts at: 1870 1943 3969 6822 7189 7796 1870
Size: 73 2026 2853 367 607 4545
Fragments arranged by size:
4545 2853 2026 607 367 73
BamHI G'GATC_C
Cuts at: 6541 7801 8168 6541
Size: 1260 367 8844
Fragments arranged by size:
8844 1260 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3667 7454 7792 9247 618
Size: 325 217 627 1880 3787 338 1455 1842
Fragments arranged by size:
3787 1880 1842 1455 627 338 325 217
BanII G_rGCy'C
Cuts at: 1391 5221 5846 6298 7299 7729 7790 1391
Size: 3830 625 452 1001 430 61 4072
Fragments arranged by size:
4072 3830 1001 625 452 430 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2219 3732 4225 7176 928
Size: 971 320 1513 493 2951 4223
Fragments arranged by size:
4223 2951 1513 971 493 320
BbvCI CC'TCA_GC
Cuts at: 2030 2030
Size: 10471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4227 4261 8662 8696 1903
Size: 34 2290 34 4401 34 3678
Fragments arranged by size:
4401 3678 2290 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3691 8363 9573 3691
Size: 4672 1210 4589
Fragments arranged by size:
4672 4589 1210
BclI T'GATC_A
Cuts at: 1548 4297 4822 6340 6955 1548
Size: 2749 525 1518 615 5064
Fragments arranged by size:
5064 2749 1518 615 525
BfrBI ATG'CAT
Cuts at: 2396 2396
Size: 10471
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9082 136
Size: 107 122 71 631 605 7410 1525
Fragments arranged by size:
7410 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2356 3756 5515 7376 10466 2356
Size: 1400 1759 1861 3090 2361
Fragments arranged by size:
3090 2361 1861 1759 1400
BlpI GC'TnA_GC
Cuts at: 5474 6741 7888 5474
Size: 1267 1147 8057
Fragments arranged by size:
8057 1267 1147
Bme1580I G_kGCm'C
Cuts at: 1790 3670 6818 8532 9688 1790
Size: 1880 3148 1714 1156 2573
Fragments arranged by size:
3148 2573 1880 1714 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10471
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4728 6770 8202
Size: 416 53 83 399 24 3311 2042 1432
Cuts at: 8202 9160 442
Size: 958 1753
Fragments arranged by size:
3311 2042 1753 1432 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10471
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 6756 3109
Size: 3647 6824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4491 4648 5960 6353 7040 7505 9131 1239
Size: 3252 157 1312 393 687 465 1626 2579
Fragments arranged by size:
3252 2579 1626 1312 687 465 393 157
Bpu10I CC'TnA_GC
Cuts at: 2030 2206 7293 7370 2030
Size: 176 5087 77 5131
Fragments arranged by size:
5131 5087 176 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 3781 4553 5801 5848 7355 8596 9461 9759
Size: 1436 772 1248 47 1507 1241 865 298
Cuts at: 9759 2345
Size: 3057
Fragments arranged by size:
3057 1507 1436 1248 1241 865 772 298
47
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3533 4196 9134 882
Size: 617 2034 663 4938 2219
Fragments arranged by size:
4938 2219 2034 663 617
BsaAI yAC'GTr
Cuts at: 493 1204 8262 493
Size: 711 7058 2702
Fragments arranged by size:
7058 2702 711
BsaBI GATnn'nnATC
Cuts at: 4284 10465 4284
Size: 6181 4290
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8278 8660 9295 275
Size: 53 83 186 936 6745 382 635 1451
Fragments arranged by size:
6745 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8904 9302 9576 9723 10134 8904
Size: 398 274 147 411 9241
Fragments arranged by size:
9241 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6378 6408 1178
Size: 30 165 30 4975 30 5241
Fragments arranged by size:
5241 4975 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3032 4190 5222 5332 5416 5851 6272 6275
Size: 1846 1158 1032 110 84 435 421 3
Cuts at: 6275 6284 6287 6290 7397 1186
Size: 9 3 3 1107 4260
Fragments arranged by size:
4260 1846 1158 1107 1032 435 421 110
84 9 3 3 3
BseYI C'CCAG_C
Cuts at: 5168 5691 5696 6101 6703 9674 10170 5168
Size: 523 5 405 602 2971 496 5469
Fragments arranged by size:
5469 2971 602 523 496 405 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2976 3008 4094 4578 5385 7343 1163
Size: 573 1240 32 1086 484 807 1958 4291
Fragments arranged by size:
4291 1958 1240 1086 807 573 484 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7921 8682 9710 1540
Size: 275 186 5920 761 1028 2301
Fragments arranged by size:
5920 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3090 3535 5817 6777 7299 8532 8617 9688 3090
Size: 445 2282 960 522 1233 85 1071 3873
Fragments arranged by size:
3873 2282 1233 1071 960 522 445 85
BsmI GAATG_Cn'
Cuts at: 4728 7982 8075 4728
Size: 3254 93 7124
Fragments arranged by size:
7124 3254 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3090 3535 3670 5221 5817 5846 6298
Size: 399 1300 445 135 1551 596 29 452
Cuts at: 6298 6777 6818 7299 7729 7790 8532 8617 9688
Size: 479 41 481 430 61 742 85 1071
Cuts at: 9688 1391
Size: 2174
Fragments arranged by size:
2174 1551 1300 1071 742 596 481 479
452 445 430 399 135 85 61 41
29
BspEI T'CCGG_A
Cuts at: 10134 10134
Size: 10471
BspHI T'CATG_A
Cuts at: 2052 2116 5440 8360 10090 2052
Size: 64 3324 2920 1730 2433
Fragments arranged by size:
3324 2920 2433 1730 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3106 3555 4600 7215 7489 7826 844
Size: 2262 449 1045 2615 274 337 3489
Fragments arranged by size:
3489 2615 2262 1045 449 337 274
BsrBI CCG'CTC
Cuts at: 1394 1823 5944 8358 1394
Size: 429 4121 2414 3507
Fragments arranged by size:
4121 3507 2414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6534 8966 9140 65
Size: 1183 5286 2432 174 1396
Fragments arranged by size:
5286 2432 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9115 9302 1700
Size: 110 7305 187 2869
Fragments arranged by size:
7305 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2866 4458 4854 96
Size: 2770 1592 396 5713
Fragments arranged by size:
5713 2770 1592 396
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10471
BssSI C'ACGA_G
Cuts at: 3873 8531 9543 3873
Size: 4658 1012 4801
Fragments arranged by size:
4801 4658 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 10471
BstBI TT'CG_AA
Cuts at: 2900 9275 2900
Size: 6375 4096
BstEII G'GTnAC_C
Cuts at: 1184 4477 1184
Size: 3293 7178
BstXI CCAn_nnnn'nTGG
Cuts at: 4672 6109 7465 4672
Size: 1437 1356 7678
Fragments arranged by size:
7678 1437 1356
BstYI r'GATC_y
Cuts at: 1972 2356 3406 3756 5515 6541 7376 7801 8168
Size: 384 1050 350 1759 1026 835 425 367
Cuts at: 8168 8553 8570 10022 10108 10120 10466 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1759 1452 1050 1026 835 425 385
384 367 350 346 86 17 12
BstZ17I GTA'TAC
Cuts at: 2742 4746 2742
Size: 2004 8467
Bsu36I CC'TnA_GG
Cuts at: 3059 6469 7755 3059
Size: 3410 1286 5775
Fragments arranged by size:
5775 3410 1286
BtgI C'CryG_G
Cuts at: 513 1065 2096 7175 7425 513
Size: 552 1031 5079 250 3559
Fragments arranged by size:
5079 3559 1031 552 250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5247 5715 7227 487
Size: 683 56 4021 468 1512 3731
Fragments arranged by size:
4021 3731 1512 683 468 56
BtsI GCAGTG_nn'
Cuts at: 4250 4961 5056 5188 6665 7987 8781 8801 4250
Size: 711 95 132 1477 1322 794 20 5920
Fragments arranged by size:
5920 1477 1322 794 711 132 95 20
ClaI AT'CG_AT
Cuts at: 1554 3634 4834 1554
Size: 2080 1200 7191
Fragments arranged by size:
7191 2080 1200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10436
DraI TTT'AAA
Cuts at: 2634 4606 4687 4771 5973 7675 7775 2634
Size: 1972 81 84 1202 1702 100 5330
Fragments arranged by size:
5330 1972 1702 1202 100 84 81
DraIII CAC_nnn'GTG
Cuts at: 3080 3080
Size: 10471
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2726 7634 9478 809
Size: 507 1410 4908 1844 1802
Fragments arranged by size:
4908 1844 1802 1410 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3891 4422 5778
Size: 54 1425 50 275 18 2061 531 1356
Cuts at: 5778 6882 7520 7918 8807 10436 8
Size: 1104 638 398 889 1629 43
Fragments arranged by size:
2061 1629 1425 1356 1104 889 638 531
398 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7918 1537
Size: 275 6106 4090
Fragments arranged by size:
6106 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3078 3504 3567 3652 4032 4167 4194 5054
Size: 1725 426 63 85 380 135 27 860
Cuts at: 5054 5350 6300 6901 7304 7386 7395 7717 8401
Size: 296 950 601 403 82 9 322 684
Cuts at: 8401 1353
Size: 3423
Fragments arranged by size:
3423 1725 950 860 684 601 426 403
380 322 296 135 85 82 63 27
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 9058 9432 9578 387
Size: 1064 138 2798 4671 374 146 1280
Fragments arranged by size:
4671 2798 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 7297 7297
Size: 10471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 4410 4491 4648 4734 5331 5882
Size: 393 622 2156 81 157 86 597 551
Cuts at: 5882 5960 6353 6788 7040 7505 8534 9131 9918
Size: 78 393 435 252 465 1029 597 787
Cuts at: 9918 10186 1239
Size: 268 1524
Fragments arranged by size:
2156 1524 1029 787 622 597 597 551
465 435 393 393 268 252 157 86
81 78
EcoNI CCTnn'n_nnAGG
Cuts at: 7055 7055
Size: 10471
EcoO109I rG'GnC_Cy
Cuts at: 2078 2945 6808 7195 2078
Size: 867 3863 387 5354
Fragments arranged by size:
5354 3863 867 387
EcoRI G'AATT_C
Cuts at: 3494 7118 7780 8158 3494
Size: 3624 662 378 5807
Fragments arranged by size:
5807 3624 662 378
EcoRV GAT'ATC
Cuts at: 4811 4811
Size: 10471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2034 2852 3533 3746 4196 2034
Size: 818 681 213 450 8309
Fragments arranged by size:
8309 818 681 450 213
FspI TGC'GCA
Cuts at: 6018 8977 6018
Size: 2959 7512
FspAI rTGC'GCAy
Cuts at: 6018 6018
Size: 10471
HaeII r_GCGC'y
Cuts at: 1678 5039 7114 9618 1678
Size: 3361 2075 2504 2531
Fragments arranged by size:
3361 2531 2504 2075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3867 3899 4967 4999 5303
Size: 32 135 32 2295 32 1068 32 304
Cuts at: 5303 5335 5435 5467 6500 6532 7196 7228 9112
Size: 32 100 32 1033 32 664 32 1884
Cuts at: 9112 9144 9186 9218 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2295 1884 1068 1033 664 304 135
100 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 6616 7225 7815 8061 669
Size: 988 281 367 4311 609 590 246 3079
Fragments arranged by size:
4311 3079 988 609 590 367 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10471
HpaI GTT'AAC
Cuts at: 8061 8061
Size: 10471
KpnI G_GTAC'C
Cuts at: 7796 7796
Size: 10471
MfeI C'AATT_G
Cuts at: 5382 8070 5382
Size: 2688 7783
MluI A'CGCG_T
Cuts at: 10347 10347
Size: 10471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 9585 9769 1305
Size: 8280 184 2007
Fragments arranged by size:
8280 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6884 10
Size: 54 1425 343 5052 3597
Fragments arranged by size:
5052 3597 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5143 5409 6113 6442 6943 6963 7164
Size: 648 3977 266 704 329 501 20 201
Cuts at: 7164 7463 8429 8788 8947 518
Size: 299 966 359 159 2042
Fragments arranged by size:
3977 2042 966 704 648 501 359 329
299 266 201 159 20
MspA1I CmG'CkG
Cuts at: 1606 2339 5351 6723 7177 7887 8564 9712 9957
Size: 733 3012 1372 454 710 677 1148 245
Cuts at: 9957 1606
Size: 2120
Fragments arranged by size:
3012 2120 1372 1148 733 710 677 454
245
NcoI C'CATG_G
Cuts at: 513 1065 2096 7425 513
Size: 552 1031 5329 3559
Fragments arranged by size:
5329 3559 1031 552
NdeI CA'TA_TG
Cuts at: 387 3172 3844 6439 387
Size: 2785 672 2595 4419
Fragments arranged by size:
4419 2785 2595 672
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10471
NotI GC'GGCC_GC
Cuts at: 7918 7918
Size: 10471
NsiI A_TGCA'T
Cuts at: 2398 2398
Size: 10471
NspI r_CATG'y
Cuts at: 1132 1229 3074 5981 6226 7073 7829 9374 1132
Size: 97 1845 2907 245 847 756 1545 2229
Fragments arranged by size:
2907 2229 1845 1545 847 756 245 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10471
PciI A'CATG_T
Cuts at: 9370 9370
Size: 10471
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10471
PfoI T'CCnGG_A
Cuts at: 4213 5884 6330 4213
Size: 1671 446 8354
Fragments arranged by size:
8354 1671 446
PmeI GTTT'AAAC
Cuts at: 7775 7775
Size: 10471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2189 2221 5413 5445 8540 8572 10053 10085 2189
Size: 32 3192 32 3095 32 1481 32 2575
Fragments arranged by size:
3192 3095 2575 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2078 2945 7195 2078
Size: 867 4250 5354
Fragments arranged by size:
5354 4250 867
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10471
PsiI TTA'TAA
Cuts at: 4920 8041 4920
Size: 3121 7350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2922 2954 2922
Size: 32 10439
PstI C_TGCA'G
Cuts at: 830 2049 5088 5206 5465 7714 7823 830
Size: 1219 3039 118 259 2249 109 3478
Fragments arranged by size:
3478 3039 2249 1219 259 118 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10471
PvuII CAG'CTG
Cuts at: 2339 5351 6723 2339
Size: 3012 1372 6087
Fragments arranged by size:
6087 3012 1372
SacI G_AGCT'C
Cuts at: 7299 7299
Size: 10471
SacII CC_GC'GG
Cuts at: 7178 7178
Size: 10471
SalI G'TCGA_C
Cuts at: 1936 7813 1936
Size: 5877 4594
SanDI GG'GwC_CC
Cuts at: 2945 2945
Size: 10471
SapI GCTCTTCn'nnn_
Cuts at: 3078 3652 6901 7304 7717 3078
Size: 574 3249 403 413 5832
Fragments arranged by size:
5832 3249 574 413 403
SbfI CC_TGCA'GG
Cuts at: 7823 7823
Size: 10471
ScaI AGT'ACT
Cuts at: 2229 7274 8719 10431 2229
Size: 5045 1445 1712 2269
Fragments arranged by size:
5045 2269 1712 1445
SexAI A'CCwGG_T
Cuts at: 2316 2439 2721 4222 2316
Size: 123 282 1501 8565
Fragments arranged by size:
8565 1501 282 123
SfcI C'TryA_G
Cuts at: 826 1045 2045 2990 4611 5084 5202 5461 6511
Size: 219 1000 945 1621 473 118 259 1050
Cuts at: 6511 7027 7710 7819 8954 9635 9826 826
Size: 516 683 109 1135 681 191 1471
Fragments arranged by size:
1621 1471 1135 1050 1000 945 683 681
516 473 259 219 191 118 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10471
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10471
SmaI CCC'GGG
Cuts at: 1872 7191 7798 1872
Size: 5319 607 4545
Fragments arranged by size:
5319 4545 607
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 2693 3760 4532 5816 5863
Size: 197 926 417 333 1067 772 1284 47
Cuts at: 5863 7334 8575 9476 9738 820
Size: 1471 1241 901 262 1553
Fragments arranged by size:
1553 1471 1284 1241 1067 926 901 772
417 333 262 197 47
SnaBI TAC'GTA
Cuts at: 493 8262 493
Size: 7769 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10471
SphI G_CATG'C
Cuts at: 1132 5981 6226 7829 1132
Size: 4849 245 1603 3774
Fragments arranged by size:
4849 3774 1603 245
SspI AAT'ATT
Cuts at: 5 52 4916 6123 8395 5
Size: 47 4864 1207 2272 2081
Fragments arranged by size:
4864 2272 2081 1207 47
StuI AGG'CCT
Cuts at: 1517 2043 3856 1517
Size: 526 1813 8132
Fragments arranged by size:
8132 1813 526
StyI C'CwwG_G
Cuts at: 513 1065 2096 5596 6079 7425 7910 513
Size: 552 1031 3500 483 1346 485 3074
Fragments arranged by size:
3500 3074 1346 1031 552 485 483
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3685 8498 8515 8668 8853 9298 325
Size: 1255 2105 4813 17 153 185 445 1498
Fragments arranged by size:
4813 2105 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2227 2866 4458
Size: 274 80 33 51 1443 250 639 1592
Cuts at: 4458 4736 4854 4929 5708 7272 7503 8717 10429
Size: 278 118 75 779 1564 231 1214 1712
Cuts at: 10429 96
Size: 138
Fragments arranged by size:
1712 1592 1564 1443 1214 779 639 278
274 250 231 138 118 80 75 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 6515 6547 10431 10463 5757
Size: 32 726 32 3884 32 5765
Fragments arranged by size:
5765 3884 726 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5911 1316
Size: 4595 5876
XbaI T'CTAG_A
Cuts at: 5431 7807 5431
Size: 2376 8095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5142 6109 5142
Size: 967 9504
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10471
XmaI C'CCGG_G
Cuts at: 1870 7189 7796 1870
Size: 5319 607 4545
Fragments arranged by size:
5319 4545 607
XmnI GAAnn'nnTTC
Cuts at: 2112 2350 2670 7360 8600 2112
Size: 238 320 4690 1240 3983
Fragments arranged by size:
4690 3983 1240 320 238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8279 276
Size: 53 83 186 936 6745 2468
Fragments arranged by size:
6745 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
FseI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI RsrII SfiI SfoI SrfI SwaI