Restriction map for
pFN21AA0914
(Circular) (Six-base) MAPSORT of: pFN21AA0914.seq Check: 4961 from: 1 to: 6712
pFN21AA0914 6712 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3028 3502 3028
Size: 474 6238
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4055 1937
Size: 2118 4594
Acc65I G'GTAC_C
Cuts at: 3537 4033 3537
Size: 496 6216
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3228 3942 4775 6159 6427 1632
Size: 1596 714 833 1384 268 1917
Fragments arranged by size:
1917 1596 1384 833 714 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 5611 6588 1203
Size: 4408 977 1327
Fragments arranged by size:
4408 1327 977
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 2927 1166
Size: 1761 4951
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6712
AlwNI CAG_nnn'CTG
Cuts at: 2207 2848 4455 6027 2207
Size: 641 1607 1572 2892
Fragments arranged by size:
2892 1607 1572 641
ApaLI G'TGCA_C
Cuts at: 3663 4769 5925 3663
Size: 1106 1156 4450
Fragments arranged by size:
4450 1156 1106
ApoI r'AATT_y
Cuts at: 1493 3167 4021 4247 4399 6508 1493
Size: 1674 854 226 152 2109 1697
Fragments arranged by size:
2109 1697 1674 854 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 3564 4037 1870
Size: 73 1621 473 4545
Fragments arranged by size:
4545 1621 473 73
BamHI G'GATC_C
Cuts at: 4042 4409 4042
Size: 367 6345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2829 3537 4033 5488 618
Size: 325 217 627 1042 708 496 1455 1842
Fragments arranged by size:
1842 1455 1042 708 627 496 325 217
BanII G_rGCy'C
Cuts at: 1391 2609 4031 1391
Size: 1218 1422 4072
Fragments arranged by size:
4072 1422 1218
BbeI G_GCGC'C
Cuts at: 2833 2833
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 3615 3867 3909 928
Size: 971 963 753 252 42 3731
Fragments arranged by size:
3731 971 963 753 252 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3644 3678 4903 4937 1903
Size: 34 1707 34 1225 34 3678
Fragments arranged by size:
3678 1707 1225 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5323 136
Size: 107 122 71 631 605 3651 1525
Fragments arranged by size:
3651 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 4129 4129
Size: 6712
Bme1580I G_kGCm'C
Cuts at: 1790 2709 3667 4773 5929 1790
Size: 919 958 1106 1156 2573
Fragments arranged by size:
2573 1156 1106 958 919
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2704 2755 3321
Size: 416 53 83 399 24 1287 51 566
Cuts at: 3321 4443 5401 442
Size: 1122 958 1753
Fragments arranged by size:
1753 1287 1122 958 566 416 399 83
53 51 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2199 2427 3350 3791 3989 5372 1239
Size: 960 228 923 441 198 1383 2579
Fragments arranged by size:
2579 1383 960 923 441 228 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3296 4837 5702 6000 3296
Size: 1541 865 298 4008
Fragments arranged by size:
4008 1541 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5375 882
Size: 617 3876 2219
Fragments arranged by size:
3876 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4503 493
Size: 711 3299 2702
Fragments arranged by size:
3299 2702 711
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2830 4519 4901 5536
Size: 53 83 186 936 1297 1689 382 635
Cuts at: 5536 275
Size: 1451
Fragments arranged by size:
1689 1451 1297 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2111 3063 3133 3171 5145 5543 5817 5964 6375
Size: 952 70 38 1974 398 274 147 411
Cuts at: 6375 2111
Size: 2448
Fragments arranged by size:
2448 1974 952 411 398 274 147 70
38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3777 3807 3948 3978 1178
Size: 30 165 30 2374 30 141 30 3912
Fragments arranged by size:
3912 2374 165 141 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2699 2761 2868 2968 3611 1186
Size: 1513 62 107 100 643 4287
Fragments arranged by size:
4287 1513 643 107 100 62
BseYI C'CCAG_C
Cuts at: 2633 2825 3024 5915 6411 2633
Size: 192 199 2891 496 2934
Fragments arranged by size:
2934 2891 496 199 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6139
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3491 4162 4923 5951 1540
Size: 275 186 1490 671 761 1028 2301
Fragments arranged by size:
2301 1490 1028 761 671 275 186
BsiHKAI G_wGCw'C
Cuts at: 2080 3667 3978 4773 4858 5929 2080
Size: 1587 311 795 85 1071 2863
Fragments arranged by size:
2863 1587 1071 795 311 85
BsmI GAATG_Cn'
Cuts at: 3477 4223 4316 3477
Size: 746 93 5873
Fragments arranged by size:
5873 746 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2609 2709 3667 3978 4031 4773
Size: 399 290 529 100 958 311 53 742
Cuts at: 4773 4858 5929 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 958 742 529 399 311 290
100 85 53
BspEI T'CCGG_A
Cuts at: 3063 3133 3171 6375 3063
Size: 70 38 3204 3400
Fragments arranged by size:
3400 3204 70 38
BspHI T'CATG_A
Cuts at: 2461 2515 2961 4601 6331 2461
Size: 54 446 1640 1730 2842
Fragments arranged by size:
2842 1730 1640 446 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3028 3502 4067 844
Size: 2184 474 565 3489
Fragments arranged by size:
3489 2184 565 474
BsrBI CCG'CTC
Cuts at: 1394 1823 3380 4599 1394
Size: 429 1557 1219 3507
Fragments arranged by size:
3507 1557 1219 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2104 3625 5207 5381 65
Size: 1183 856 1521 1582 174 1396
Fragments arranged by size:
1582 1521 1396 1183 856 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2672 3490 3540 5356 5543 1700
Size: 110 862 818 50 1816 187 2869
Fragments arranged by size:
2869 1816 862 818 187 110 50
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6712
BssSI C'ACGA_G
Cuts at: 4772 5784 4772
Size: 1012 5700
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2228 794
Size: 1434 5278
BstBI TT'CG_AA
Cuts at: 3084 3687 3838 5516 3084
Size: 603 151 1678 4280
Fragments arranged by size:
4280 1678 603 151
BstEII G'GTnAC_C
Cuts at: 1184 2675 1184
Size: 1491 5221
BstXI CCAn_nnnn'nTGG
Cuts at: 4009 4009
Size: 6712
BstYI r'GATC_y
Cuts at: 1972 2619 3060 3555 4042 4409 4794 4811 6263
Size: 647 441 495 487 367 385 17 1452
Cuts at: 6263 6349 6361 6707 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 647 495 487 441 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3396 3396
Size: 6712
BtgI C'CryG_G
Cuts at: 513 1065 2004 4008 513
Size: 552 939 2004 3217
Fragments arranged by size:
3217 2004 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5973
Fragments arranged by size:
5973 683 56
BtsI GCAGTG_nn'
Cuts at: 4228 5022 5042 4228
Size: 794 20 5898
Fragments arranged by size:
5898 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3638 5719 809
Size: 507 2322 2081 1802
Fragments arranged by size:
2322 2081 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3488 4159 5048
Size: 54 1425 50 275 18 1658 671 889
Cuts at: 5048 6677 8
Size: 1629 43
Fragments arranged by size:
1658 1629 1425 889 671 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3488 4159 1537
Size: 275 1676 671 4090
Fragments arranged by size:
4090 1676 671 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2745 3084 3204 3588 4642 1353
Size: 1392 339 120 384 1054 3423
Fragments arranged by size:
3423 1392 1054 384 339 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5299 5673 5819 387
Size: 1064 138 3710 374 146 1280
Fragments arranged by size:
3710 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2427 3228 3350 3791 3942 3989
Size: 393 567 228 801 122 441 151 47
Cuts at: 3989 4775 5372 6159 6427 1239
Size: 786 597 787 268 1524
Fragments arranged by size:
1524 801 787 786 597 567 441 393
268 228 151 122 47
EcoO109I rG'GnC_Cy
Cuts at: 2988 3010 3214 2988
Size: 22 204 6486
Fragments arranged by size:
6486 204 22
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
EcoRV GAT'ATC
Cuts at: 3204 3204
Size: 6712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3649 3649
Size: 6712
FspI TGC'GCA
Cuts at: 5218 5218
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2251 2833 5859 1678
Size: 573 582 3026 2531
Fragments arranged by size:
3026 2531 582 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 3587 3619 3989
Size: 32 135 32 617 32 1366 32 370
Cuts at: 3989 4021 5353 5385 5427 5459 1373
Size: 32 1332 32 42 32 2626
Fragments arranged by size:
2626 1366 1332 617 370 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4056 4302 669
Size: 988 281 2118 246 3079
Fragments arranged by size:
3079 2118 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 2829 2829
Size: 6712
KpnI G_GTAC'C
Cuts at: 3541 4037 3541
Size: 496 6216
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 5826 6010 1305
Size: 1389 3132 184 2007
Fragments arranged by size:
3132 2007 1389 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4890
Fragments arranged by size:
4890 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2927 3739 3914 4670 5029 5188
Size: 648 1177 584 812 175 756 359 159
Cuts at: 5188 518
Size: 2042
Fragments arranged by size:
2042 1177 812 756 648 584 359 175
159
MspA1I CmG'CkG
Cuts at: 1606 2144 2782 2845 4128 4805 5953 6198 1606
Size: 538 638 63 1283 677 1148 245 2120
Fragments arranged by size:
2120 1283 1148 677 638 538 245 63
NarI GG'CG_CC
Cuts at: 2830 2830
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 2004 4008 513
Size: 552 939 2004 3217
Fragments arranged by size:
3217 2004 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6712
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 4070 5615 1132
Size: 97 2841 1545 2229
Fragments arranged by size:
2841 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6712
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6712
PfoI T'CCnGG_A
Cuts at: 2622 3366 3795 3807 3966 2622
Size: 744 429 12 159 5368
Fragments arranged by size:
5368 744 429 159 12
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2780 2812 3250 3282 4781 4813 6294 6326 2780
Size: 32 438 32 1499 32 1481 32 3166
Fragments arranged by size:
3166 1499 1481 438 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2988 3010 3214 2988
Size: 22 204 6486
Fragments arranged by size:
6486 204 22
PshAI GACnn'nnGTC
Cuts at: 1613 2056 1613
Size: 443 6269
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PstI C_TGCA'G
Cuts at: 830 3453 4064 830
Size: 2623 611 3478
Fragments arranged by size:
3478 2623 611
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2144 2845 2144
Size: 701 6011
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SapI GCTCTTCn'nnn_
Cuts at: 2745 2745
Size: 6712
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 2443 4960 6672 2443
Size: 2517 1712 2483
Fragments arranged by size:
2517 2483 1712
SfcI C'TryA_G
Cuts at: 826 1045 2145 3449 4060 5195 5876 6067 826
Size: 219 1100 1304 611 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 1100 681 611 219 191
SfoI GGC'GCC
Cuts at: 2831 2831
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 4039 1872
Size: 2167 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3275 4816 5717 5979 820
Size: 197 926 1332 1541 901 262 1553
Fragments arranged by size:
1553 1541 1332 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 4070 1132
Size: 2938 3774
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6712
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 4008 4151 513
Size: 552 939 258 1746 143 3074
Fragments arranged by size:
3074 1746 939 552 258 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4739 4756 4909 5094 5539 325
Size: 1255 3159 17 153 185 445 1498
Fragments arranged by size:
3159 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2441 3099 4958
Size: 274 80 33 51 1443 464 658 1859
Cuts at: 4958 6670 96
Size: 1712 138
Fragments arranged by size:
1859 1712 1443 658 464 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6672 6704 6672
Size: 32 6680
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6712
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 4037 1870
Size: 2167 4545
XmnI GAAnn'nnTTC
Cuts at: 3351 3477 4841 3351
Size: 126 1364 5222
Fragments arranged by size:
5222 1364 126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfiI SrfI SwaI XcmI