Restriction map for
pFN21AA0909
(Circular) (Six-base) MAPSORT of: pFN21AA0909.seq Check: 1185 from: 1 to: 8311
pFN21AA0909 8311 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3296 3430 3787 3973 3296
Size: 134 357 186 7634
Fragments arranged by size:
7634 357 186 134
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6121 278
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5654 1937
Size: 3717 4594
Acc65I G'GTAC_C
Cuts at: 5632 5632
Size: 8311
AclI AA'CG_TT
Cuts at: 1563 6438 6811 1563
Size: 4875 373 3063
Fragments arranged by size:
4875 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2345 2546 2579 3490 4737 5031 5225
Size: 440 273 201 33 911 1247 294 194
Cuts at: 5225 5582 6374 7758 8026 1632
Size: 357 792 1384 268 1917
Fragments arranged by size:
1917 1384 1247 911 792 440 357 294
273 268 201 194 33
AfeI AGC'GCT
Cuts at: 5243 5243
Size: 8311
AflII C'TTAA_G
Cuts at: 820 1017 5282 820
Size: 197 4265 3849
Fragments arranged by size:
4265 3849 197
AflIII A'CryG_T
Cuts at: 1203 7210 8187 1203
Size: 6007 977 1327
Fragments arranged by size:
6007 1327 977
AgeI A'CCGG_T
Cuts at: 7142 7142
Size: 8311
AhdI GACnn_n'nnGTC
Cuts at: 3892 7040 3892
Size: 3148 5163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4173 1184
Size: 2989 5322
AlwNI CAG_nnn'CTG
Cuts at: 3977 4351 4505 4619 4928 5231 5578 6054 7626
Size: 374 154 114 309 303 347 476 1572
Cuts at: 7626 3977
Size: 4662
Fragments arranged by size:
4662 1572 476 374 347 309 303 154
114
ApaI G_GGCC'C
Cuts at: 3819 4722 3819
Size: 903 7408
ApaLI G'TGCA_C
Cuts at: 2435 6368 7524 2435
Size: 3933 1156 3222
Fragments arranged by size:
3933 3222 1156
ApoI r'AATT_y
Cuts at: 1493 5620 5846 5998 8107 1493
Size: 4127 226 152 2109 1697
Fragments arranged by size:
4127 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6865 160
Size: 872 5833 1606
Fragments arranged by size:
5833 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
AvaI C'yCGr_G
Cuts at: 1870 1943 2304 2535 2772 2946 3206 3279 4401
Size: 73 361 231 237 174 260 73 1122
Cuts at: 4401 4892 5160 5636 1870
Size: 491 268 476 4545
Fragments arranged by size:
4545 1122 491 476 361 268 260 237
231 174 73 73
AvrII C'CTAG_G
Cuts at: 3053 3053
Size: 8311
BamHI G'GATC_C
Cuts at: 5641 6008 5641
Size: 367 7944
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3985 4547 5632 7087 618
Size: 325 217 627 2198 562 1085 1455 1842
Fragments arranged by size:
2198 1842 1455 1085 627 562 325 217
BanII G_rGCy'C
Cuts at: 1391 2772 2853 2916 3267 3363 3504 3819 3853
Size: 1381 81 63 351 96 141 315 34
Cuts at: 3853 4467 4722 4938 5630 1391
Size: 614 255 216 692 4072
Fragments arranged by size:
4072 1381 692 614 351 315 255 216
141 96 81 63 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2078 2303 928
Size: 971 179 225 6936
Fragments arranged by size:
6936 971 225 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6502 6536 1903
Size: 34 4565 34 3678
Fragments arranged by size:
4565 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6203 7413 6203
Size: 1210 7101
BclI T'GATC_A
Cuts at: 1548 2157 3709 1548
Size: 609 1552 6150
Fragments arranged by size:
6150 1552 609
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3028 6922 136
Size: 107 122 71 631 605 1356 3894 1525
Fragments arranged by size:
3894 1525 1356 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2055 8306 2055
Size: 6251 2060
BlpI GC'TnA_GC
Cuts at: 5728 5728
Size: 8311
Bme1580I G_kGCm'C
Cuts at: 1790 2197 2439 3819 4300 4582 4722 6372 7528
Size: 407 242 1380 481 282 140 1650 1156
Cuts at: 7528 1790
Size: 2573
Fragments arranged by size:
2573 1650 1380 1156 481 407 282 242
140
BmgBI CAC'GTC
Cuts at: 1318 4563 1318
Size: 3245 5066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 6042 7000
Size: 416 53 83 399 24 1694 2931 958
Cuts at: 7000 442
Size: 1753
Fragments arranged by size:
2931 1753 1694 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8311
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3712 5089 3712
Size: 1377 6934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2094 2099 2396 2480 3017 3030 3281 3518
Size: 855 5 297 84 537 13 251 237
Cuts at: 3518 3959 3996 4477 4755 4920 4991 6971 1239
Size: 441 37 481 278 165 71 1980 2579
Fragments arranged by size:
2579 1980 855 537 481 441 297 278
251 237 165 84 71 37 13 5
Bpu10I CC'TnA_GC
Cuts at: 4193 4193
Size: 8311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3347 4451 6436 7301 7599 3347
Size: 1104 1985 865 298 4059
Fragments arranged by size:
4059 1985 1104 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4181 4937 6974 882
Size: 617 2682 756 2037 2219
Fragments arranged by size:
2682 2219 2037 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 6102 493
Size: 711 4898 2702
Fragments arranged by size:
4898 2702 711
BsaBI GATnn'nnATC
Cuts at: 8305 8305
Size: 8311
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3020 6118 6500 7135
Size: 53 83 186 936 1487 3098 382 635
Cuts at: 7135 275
Size: 1451
Fragments arranged by size:
3098 1487 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3915 4855 5250 5442 6744 7142 7416 7563 7974
Size: 940 395 192 1302 398 274 147 411
Cuts at: 7974 3915
Size: 4252
Fragments arranged by size:
4252 1302 940 411 398 395 274 192
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 3465 3495 5396
Size: 30 165 30 1128 30 904 30 1901
Cuts at: 5396 5426 5462 5492 1178
Size: 30 36 30 3997
Fragments arranged by size:
3997 1901 1128 904 165 36 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2555 2811 2884 2972 2979 3102 3371
Size: 967 402 256 73 88 7 123 269
Cuts at: 3371 3471 3999 4480 4513 4676 4717 1186
Size: 100 528 481 33 163 41 4780
Fragments arranged by size:
4780 967 528 481 402 269 256 163
123 100 88 73 41 33 7
BseYI C'CCAG_C
Cuts at: 2452 3582 3977 4991 5006 5592 7514 8010 2452
Size: 1130 395 1014 15 586 1922 496 2753
Fragments arranged by size:
2753 1922 1130 1014 586 496 395 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2651 4133 4587 1163
Size: 573 915 1482 454 4887
Fragments arranged by size:
4887 1482 915 573 454
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2512 5761 6522 7550 1540
Size: 275 186 511 3249 761 1028 2301
Fragments arranged by size:
3249 2301 1028 761 511 275 186
BsiHKAI G_wGCw'C
Cuts at: 2439 2853 3363 3660 3837 4326 5394 6372 6457
Size: 414 510 297 177 489 1068 978 85
Cuts at: 6457 7528 2439
Size: 1071 3222
Fragments arranged by size:
3222 1071 1068 978 510 489 414 297
177 85
BsmI GAATG_Cn'
Cuts at: 4356 5822 5915 4356
Size: 1466 93 6752
Fragments arranged by size:
6752 1466 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4525 4525
Size: 8311
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2197 2439 2772 2853 2916 3267 3363
Size: 399 407 242 333 81 63 351 96
Cuts at: 3363 3504 3660 3819 3837 3853 4300 4326 4467
Size: 141 156 159 18 16 447 26 141
Cuts at: 4467 4582 4722 4938 5394 5630 6372 6457 7528
Size: 115 140 216 456 236 742 85 1071
Cuts at: 7528 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 456 447 407 399 351
333 242 236 216 159 156 141 141
140 115 96 85 81 63 26 18
16
BspEI T'CCGG_A
Cuts at: 3915 7974 3915
Size: 4059 4252
BspHI T'CATG_A
Cuts at: 5520 6200 7930 5520
Size: 680 1730 5901
Fragments arranged by size:
5901 1730 680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3296 3430 3787 3973 5666 844
Size: 2452 134 357 186 1693 3489
Fragments arranged by size:
3489 2452 1693 357 186 134
BsrBI CCG'CTC
Cuts at: 1394 1823 3221 4252 4861 4918 5384 5412 6198
Size: 429 1398 1031 609 57 466 28 786
Cuts at: 6198 1394
Size: 3507
Fragments arranged by size:
3507 1398 1031 786 609 466 429 57
28
BsrDI GCAATG_nn'
Cuts at: 65 1248 6806 6980 65
Size: 1183 5558 174 1396
Fragments arranged by size:
5558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5196 6955 7142 1700
Size: 110 3386 1759 187 2869
Fragments arranged by size:
3386 2869 1759 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8311
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8311
BssSI C'ACGA_G
Cuts at: 5132 6371 7383 5132
Size: 1239 1012 6060
Fragments arranged by size:
6060 1239 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8311
BstBI TT'CG_AA
Cuts at: 4044 7115 4044
Size: 3071 5240
BstEII G'GTnAC_C
Cuts at: 1184 2267 1184
Size: 1083 7228
BstXI CCAn_nnnn'nTGG
Cuts at: 3034 3070 3173 3496 4037 4969 3034
Size: 36 103 323 541 932 6376
Fragments arranged by size:
6376 932 541 323 103 36
BstYI r'GATC_y
Cuts at: 1972 2055 5641 6008 6393 6410 7862 7948 7960
Size: 83 3586 367 385 17 1452 86 12
Cuts at: 7960 8306 1972
Size: 346 1977
Fragments arranged by size:
3586 1977 1452 385 367 346 86 83
17 12
BtgI C'CryG_G
Cuts at: 513 1065 4117 513
Size: 552 3052 4707
Fragments arranged by size:
4707 3052 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3700 487
Size: 683 56 2474 5098
Fragments arranged by size:
5098 2474 683 56
BtsI GCAGTG_nn'
Cuts at: 2698 2970 3081 3726 5827 6621 6641 2698
Size: 272 111 645 2101 794 20 4368
Fragments arranged by size:
4368 2101 794 645 272 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2011 2046 529
Size: 35 1447 35 6794
Fragments arranged by size:
6794 1447 35 35
DraI TTT'AAA
Cuts at: 5615 5615
Size: 8311
DraIII CAC_nnn'GTG
Cuts at: 2629 2629
Size: 8311
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7318 809
Size: 507 6002 1802
Fragments arranged by size:
6002 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4959 5316 5451
Size: 54 1425 50 275 18 3129 357 135
Cuts at: 5451 5604 5758 6647 8276 8
Size: 153 154 889 1629 43
Fragments arranged by size:
3129 1629 1425 889 357 275 154 153
135 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5758 1537
Size: 275 3946 4090
Fragments arranged by size:
4090 3946 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2374 2601 2890 3310 3448 6241 1353
Size: 1021 227 289 420 138 2793 3423
Fragments arranged by size:
3423 2793 1021 420 289 227 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 6898 7272 7418 387
Size: 1064 138 2630 2679 374 146 1280
Fragments arranged by size:
2679 2630 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2851 3361 2851
Size: 510 7801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2094 2099 2345 2396 2480 2546
Size: 393 440 22 5 246 51 84 66
Cuts at: 2546 2579 3017 3030 3281 3490 3518 3959 3996
Size: 33 438 13 251 209 28 441 37
Cuts at: 3996 4477 4737 4755 4920 4991 5031 5225 5582
Size: 481 260 18 165 71 40 194 357
Cuts at: 5582 6374 6971 7758 8026 1239
Size: 792 597 787 268 1524
Fragments arranged by size:
1524 792 787 597 481 441 440 438
393 357 268 260 251 246 209 194
165 84 71 66 51 40 37 33
28 22 18 13 5
EcoO109I rG'GnC_Cy
Cuts at: 3284 3476 3668 3815 3816 3998 4034 4100 4718
Size: 192 192 147 1 182 36 66 618
Cuts at: 4718 4719 3284
Size: 1 6876
Fragments arranged by size:
6876 618 192 192 182 147 66 36
1 1
EcoRI G'AATT_C
Cuts at: 5620 5998 5620
Size: 378 7933
FspI TGC'GCA
Cuts at: 6817 6817
Size: 8311
HaeII r_GCGC'y
Cuts at: 1678 2398 5245 5539 7458 1678
Size: 720 2847 294 1919 2531
Fragments arranged by size:
2847 2531 1919 720 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2171 2194 2203
Size: 32 135 32 560 32 7 23 9
Cuts at: 2203 2226 3528 3560 3878 3910 4253 4285 4808
Size: 23 1302 32 318 32 343 32 523
Cuts at: 4808 4840 4934 4966 6952 6984 7026 7058 1373
Size: 32 94 32 1986 32 42 32 2626
Fragments arranged by size:
2626 1986 1302 560 523 343 318 135
94 42 32 32 32 32 32 32
32 32 32 32 23 23 9 7
HincII GTy'rAC
Cuts at: 669 1657 1938 4246 5655 5901 669
Size: 988 281 2308 1409 246 3079
Fragments arranged by size:
3079 2308 1409 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4222 748
Size: 3474 4837
HpaI GTT'AAC
Cuts at: 5901 5901
Size: 8311
KpnI G_GTAC'C
Cuts at: 5636 5636
Size: 8311
MfeI C'AATT_G
Cuts at: 5910 5910
Size: 8311
MluI A'CGCG_T
Cuts at: 8187 8187
Size: 8311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3599 4316 4521 7425 7609 1305
Size: 2294 717 205 2904 184 2007
Fragments arranged by size:
2904 2294 2007 717 205 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4961 5606 10
Size: 54 1425 343 3129 645 2715
Fragments arranged by size:
3129 2715 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3719 6269 6628 6787 518
Size: 648 2553 2550 359 159 2042
Fragments arranged by size:
2553 2550 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2101 2557 3004 3275 3586 3977 4425 5140
Size: 495 456 447 271 311 391 448 715
Cuts at: 5140 5231 5266 5727 6404 7552 7797 1606
Size: 91 35 461 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 495 461 456 448
447 391 311 271 245 91 35
NaeI GCC'GGC
Cuts at: 5198 5198
Size: 8311
NcoI C'CATG_G
Cuts at: 513 1065 4117 513
Size: 552 3052 4707
Fragments arranged by size:
4707 3052 552
NdeI CA'TA_TG
Cuts at: 387 3172 387
Size: 2785 5526
NgoMIV G'CCGG_C
Cuts at: 5196 5196
Size: 8311
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8311
NotI GC'GGCC_GC
Cuts at: 5758 5758
Size: 8311
NspI r_CATG'y
Cuts at: 1132 1229 5669 7214 1132
Size: 97 4440 1545 2229
Fragments arranged by size:
4440 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4165 4300 1348
Size: 2817 135 5359
Fragments arranged by size:
5359 2817 135
PciI A'CATG_T
Cuts at: 7210 7210
Size: 8311
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 3644 4505 4953 5578 1108
Size: 1230 1306 861 448 625 3841
Fragments arranged by size:
3841 1306 1230 861 625 448
PmeI GTTT'AAAC
Cuts at: 5615 5615
Size: 8311
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6380 6412 7893 7925 6380
Size: 32 1481 32 6766
Fragments arranged by size:
6766 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3284 3476 3668 3998 4100 3284
Size: 192 192 330 102 7495
Fragments arranged by size:
7495 330 192 192 102
PshAI GACnn'nnGTC
Cuts at: 1613 4506 1613
Size: 2893 5418
PsiI TTA'TAA
Cuts at: 5881 5881
Size: 8311
PspOMI G'GGCC_C
Cuts at: 3815 4718 3815
Size: 903 7408
PstI C_TGCA'G
Cuts at: 830 2483 2693 5663 830
Size: 1653 210 2970 3478
Fragments arranged by size:
3478 2970 1653 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8311
PvuII CAG'CTG
Cuts at: 2557 3275 3586 3977 5231 5266 2557
Size: 718 311 391 1254 35 5602
Fragments arranged by size:
5602 1254 718 391 311 35
SacI G_AGCT'C
Cuts at: 2853 3363 2853
Size: 510 7801
SalI G'TCGA_C
Cuts at: 1936 5653 1936
Size: 3717 4594
SanDI GG'GwC_CC
Cuts at: 3284 3284
Size: 8311
SbfI CC_TGCA'GG
Cuts at: 5663 5663
Size: 8311
ScaI AGT'ACT
Cuts at: 3877 6559 8271 3877
Size: 2682 1712 3917
Fragments arranged by size:
3917 2682 1712
SexAI A'CCwGG_T
Cuts at: 2625 2625
Size: 8311
SfcI C'TryA_G
Cuts at: 826 1045 2479 2616 2689 4439 5659 6794 7475
Size: 219 1434 137 73 1750 1220 1135 681
Cuts at: 7475 7666 826
Size: 191 1471
Fragments arranged by size:
1750 1471 1434 1220 1135 681 219 191
137 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8311
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8311
SmaI CCC'GGG
Cuts at: 1872 2537 4403 5638 1872
Size: 665 1866 1235 4545
Fragments arranged by size:
4545 1866 1235 665
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2946 3362 4430 5282 6415 7316
Size: 197 926 1003 416 1068 852 1133 901
Cuts at: 7316 7578 820
Size: 262 1553
Fragments arranged by size:
1553 1133 1068 1003 926 901 852 416
262 197
SnaBI TAC'GTA
Cuts at: 493 6102 493
Size: 5609 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8311
SphI G_CATG'C
Cuts at: 1132 5669 1132
Size: 4537 3774
SspI AAT'ATT
Cuts at: 5 52 6235 5
Size: 47 6183 2081
Fragments arranged by size:
6183 2081 47
StuI AGG'CCT
Cuts at: 1517 2209 3058 3464 1517
Size: 692 849 406 6364
Fragments arranged by size:
6364 849 692 406
StyI C'CwwG_G
Cuts at: 513 1065 2013 2856 3053 3429 3671 4117 4713
Size: 552 948 843 197 376 242 446 596
Cuts at: 4713 5750 513
Size: 1037 3074
Fragments arranged by size:
3074 1037 948 843 596 552 446 376
242 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2663 2667 3400 3692 6338 6355 6508
Size: 1255 1083 4 733 292 2646 17 153
Cuts at: 6508 6693 7138 325
Size: 185 445 1498
Fragments arranged by size:
2646 1498 1255 1083 733 445 292 185
153 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5187 5256
Size: 274 80 33 51 1443 1898 1312 69
Cuts at: 5256 6557 8269 96
Size: 1301 1712 138
Fragments arranged by size:
1898 1712 1443 1312 1301 274 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5396 5428 8271 8303 5396
Size: 32 2843 32 5404
Fragments arranged by size:
5404 2843 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2426 4948 1316
Size: 1110 2522 4679
Fragments arranged by size:
4679 2522 1110
XbaI T'CTAG_A
Cuts at: 5647 5647
Size: 8311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 3031 4089 4658 4954 5092 2074
Size: 957 1058 569 296 138 5293
Fragments arranged by size:
5293 1058 957 569 296 138
XhoI C'TCGA_G
Cuts at: 1943 2946 1943
Size: 1003 7308
XmaI C'CCGG_G
Cuts at: 1870 2535 4401 5636 1870
Size: 665 1866 1235 4545
Fragments arranged by size:
4545 1866 1235 665
XmnI GAAnn'nnTTC
Cuts at: 5586 6440 5586
Size: 854 7457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6119 276
Size: 53 83 186 936 4585 2468
Fragments arranged by size:
4585 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI