Restriction map for
pFN21AA0901
(Circular) (Six-base) MAPSORT of: pFN21AA0901.seq Check: 5410 from: 1 to: 8350
pFN21AA0901 8350 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3117 4305 3117
Size: 1188 7162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6160 278
Size: 53 83 186 936 4624 2468
Fragments arranged by size:
4624 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5457 5693 1937
Size: 3520 236 4594
Fragments arranged by size:
4594 3520 236
Acc65I G'GTAC_C
Cuts at: 3511 5671 3511
Size: 2160 6190
AclI AA'CG_TT
Cuts at: 1563 6477 6850 1563
Size: 4914 373 3063
Fragments arranged by size:
4914 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2885 3201 3255 6413 7797 8065 1632
Size: 633 620 316 54 3158 1384 268 1917
Fragments arranged by size:
3158 1917 1384 633 620 316 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8153
AflIII A'CryG_T
Cuts at: 1203 3978 5647 7249 8226 1203
Size: 2775 1669 1602 977 1327
Fragments arranged by size:
2775 1669 1602 1327 977
AgeI A'CCGG_T
Cuts at: 7181 7181
Size: 8350
AhdI GACnn_n'nnGTC
Cuts at: 7079 7079
Size: 8350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8350
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2484 2516 2531 2563 2484
Size: 32 15 32 8271
Fragments arranged by size:
8271 32 32 15
AlwNI CAG_nnn'CTG
Cuts at: 2573 3605 4494 6093 7665 2573
Size: 1032 889 1599 1572 3258
Fragments arranged by size:
3258 1599 1572 1032 889
ApaI G_GGCC'C
Cuts at: 4128 4453 4128
Size: 325 8025
ApaLI G'TGCA_C
Cuts at: 2765 3738 6407 7563 2765
Size: 973 2669 1156 3552
Fragments arranged by size:
3552 2669 1156 973
ApoI r'AATT_y
Cuts at: 1493 2291 5503 5659 5885 6037 8146 1493
Size: 798 3212 156 226 152 2109 1697
Fragments arranged by size:
3212 2109 1697 798 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 6904 160
Size: 872 5872 1606
Fragments arranged by size:
5872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 3672 5077 5675 1870
Size: 73 1056 673 1405 598 4545
Fragments arranged by size:
4545 1405 1056 673 598 73
AvrII C'CTAG_G
Cuts at: 4586 4586
Size: 8350
BamHI G'GATC_C
Cuts at: 3930 4243 5680 6047 3930
Size: 313 1437 367 6233
Fragments arranged by size:
6233 1437 367 313
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3245 3511 4034 4061 4567
Size: 325 217 627 1458 266 523 27 506
Cuts at: 4567 5048 5671 7126 618
Size: 481 623 1455 1842
Fragments arranged by size:
1842 1458 1455 627 623 523 506 481
325 266 217 27
BanII G_rGCy'C
Cuts at: 1391 3303 3402 4128 4453 5669 1391
Size: 1912 99 726 325 1216 4072
Fragments arranged by size:
4072 1912 1216 726 325 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2205 4530 928
Size: 971 306 2325 4748
Fragments arranged by size:
4748 2325 971 306
BbvCI CC'TCA_GC
Cuts at: 3012 5525 3012
Size: 2513 5837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 5260 5294 6541 6575 1903
Size: 34 2120 34 1169 34 1247 34 3678
Fragments arranged by size:
3678 2120 1247 1169 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6242 7452 6242
Size: 1210 7140
BclI T'GATC_A
Cuts at: 1548 4027 5302 1548
Size: 2479 1275 4596
Fragments arranged by size:
4596 2479 1275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2561 3083 3602
Size: 107 122 71 631 605 889 522 519
Cuts at: 3602 4077 4763 6961 136
Size: 475 686 2198 1525
Fragments arranged by size:
2198 1525 889 686 631 605 522 519
475 122 107 71
BglII A'GATC_T
Cuts at: 8345 8345
Size: 8350
BlpI GC'TnA_GC
Cuts at: 3606 4596 5767 3606
Size: 990 1171 6189
Fragments arranged by size:
6189 1171 990
Bme1580I G_kGCm'C
Cuts at: 1790 2769 3250 3265 3742 4094 4128 4453 5051
Size: 979 481 15 477 352 34 325 598
Cuts at: 5051 6411 7567 1790
Size: 1360 1156 2573
Fragments arranged by size:
2573 1360 1156 979 598 481 477 352
325 34 15
BmgBI CAC'GTC
Cuts at: 1318 2980 5389 1318
Size: 1662 2409 4279
Fragments arranged by size:
4279 2409 1662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 2467 2802
Size: 416 53 83 399 24 642 408 335
Cuts at: 2802 3717 3990 5136 6081 7039 442
Size: 915 273 1146 945 958 1753
Fragments arranged by size:
1753 1146 958 945 915 642 416 408
399 335 273 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 3170 3254 3566 4525 4665 5025 5268
Size: 829 1102 84 312 959 140 360 243
Cuts at: 5268 7010 1239
Size: 1742 2579
Fragments arranged by size:
2579 1742 1102 959 829 360 312 243
140 84
Bpu10I CC'TnA_GC
Cuts at: 2331 3012 5525 2331
Size: 681 2513 5156
Fragments arranged by size:
5156 2513 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 3317 6475 7340 7638 2258
Size: 1059 3158 865 298 2970
Fragments arranged by size:
3158 2970 1059 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3210 3326 4219 4398 4463 7013 882
Size: 617 1711 116 893 179 65 2550 2219
Fragments arranged by size:
2550 2219 1711 893 617 179 116 65
BsaAI yAC'GTr
Cuts at: 493 1204 2695 3637 6141 493
Size: 711 1491 942 2504 2702
Fragments arranged by size:
2702 2504 1491 942 711
BsaBI GATnn'nnATC
Cuts at: 8344 8344
Size: 8350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3186 6157 6539 7174
Size: 53 83 186 936 1653 2971 382 635
Cuts at: 7174 275
Size: 1451
Fragments arranged by size:
2971 1653 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2613 2736 4846 6783 7181 7455 7602 8013 2613
Size: 123 2110 1937 398 274 147 411 2950
Fragments arranged by size:
2950 2110 1937 411 398 274 147 123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 3689 3719 1178
Size: 30 165 30 1128 30 1128 30 5809
Fragments arranged by size:
5809 1128 1128 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3371 3386 3395 3563 4838 4880 4893 4964
Size: 2185 15 9 168 1275 42 13 71
Cuts at: 4964 4977 5048 5058 5237 1186
Size: 13 71 10 179 4299
Fragments arranged by size:
4299 2185 1275 179 168 71 71 42
15 13 13 10 9
BseYI C'CCAG_C
Cuts at: 2655 3096 3516 3553 4249 4804 4930 7553 8049
Size: 441 420 37 696 555 126 2623 496
Cuts at: 8049 2655
Size: 2956
Fragments arranged by size:
2956 2623 696 555 496 441 420 126
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2960 3665 4001 4218 4369 5135 1163
Size: 573 1224 705 336 217 151 766 4378
Fragments arranged by size:
4378 1224 766 705 573 336 217 151
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4081 5800 6561 7589 1540
Size: 275 186 2080 1719 761 1028 2301
Fragments arranged by size:
2301 2080 1719 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2769 3286 3411 3742 3786 5084 6411 6496 7567
Size: 517 125 331 44 1298 1327 85 1071
Cuts at: 7567 2769
Size: 3552
Fragments arranged by size:
3552 1327 1298 1071 517 331 125 85
44
BsmI GAATG_Cn'
Cuts at: 5861 5954 5861
Size: 93 8257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2769 3250 3265 3286 3303 3402 3411
Size: 399 979 481 15 21 17 99 9
Cuts at: 3411 3742 3786 4094 4128 4453 5051 5084 5669
Size: 331 44 308 34 325 598 33 585
Cuts at: 5669 6411 6496 7567 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 979 742 598 585 481 399
331 325 308 99 85 44 34 33
21 17 15 9
BspEI T'CCGG_A
Cuts at: 2613 2736 4846 8013 2613
Size: 123 2110 3167 2950
Fragments arranged by size:
3167 2950 2110 123
BspHI T'CATG_A
Cuts at: 4512 6239 7969 4512
Size: 1727 1730 4893
Fragments arranged by size:
4893 1730 1727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3117 4305 5380 5705 844
Size: 2273 1188 1075 325 3489
Fragments arranged by size:
3489 2273 1188 1075 325
BsrBI CCG'CTC
Cuts at: 1394 1823 2130 2335 4600 6237 1394
Size: 429 307 205 2265 1637 3507
Fragments arranged by size:
3507 2265 1637 429 307 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 2196 2296 2961 4179 6845 7019 65
Size: 1183 948 100 665 1218 2666 174 1396
Fragments arranged by size:
2666 1396 1218 1183 948 665 174 100
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 6994 7181 1700
Size: 110 205 4979 187 2869
Fragments arranged by size:
4979 2869 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8350
BssSI C'ACGA_G
Cuts at: 4611 6410 7422 4611
Size: 1799 1012 5539
Fragments arranged by size:
5539 1799 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2983 3746 794
Size: 2189 763 5398
Fragments arranged by size:
5398 2189 763
BstBI TT'CG_AA
Cuts at: 2096 7154 2096
Size: 5058 3292
BstEII G'GTnAC_C
Cuts at: 1184 4555 1184
Size: 3371 4979
BstXI CCAn_nnnn'nTGG
Cuts at: 3965 5337 5402 5498 3965
Size: 1372 65 96 6817
Fragments arranged by size:
6817 1372 96 65
BstYI r'GATC_y
Cuts at: 1972 2227 2610 3930 4243 5285 5680 6047 6432
Size: 255 383 1320 313 1042 395 367 385
Cuts at: 6432 6449 7901 7987 7999 8345 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1320 1042 395 385 383 367
346 313 255 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2076 2566 2579 3507 4272 2076
Size: 490 13 928 765 6154
Fragments arranged by size:
6154 928 765 490 13
BtgI C'CryG_G
Cuts at: 513 1065 2770 3336 3958 4047 4374 4430 4998
Size: 552 1705 566 622 89 327 56 568
Cuts at: 4998 5497 513
Size: 499 3366
Fragments arranged by size:
3366 1705 622 568 566 552 499 327
89 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4148 4469 5590 487
Size: 683 56 2922 321 1121 3247
Fragments arranged by size:
3247 2922 1121 683 321 56
BtsI GCAGTG_nn'
Cuts at: 3478 5330 5866 6660 6680 3478
Size: 1852 536 794 20 5148
Fragments arranged by size:
5148 1852 794 536 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8315
DraI TTT'AAA
Cuts at: 5654 5654
Size: 8350
DraIII CAC_nnn'GTG
Cuts at: 3245 3245
Size: 8350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2087 2810 7357 809
Size: 507 771 723 4547 1802
Fragments arranged by size:
4547 1802 771 723 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2182 2625
Size: 54 1425 50 275 18 187 165 443
Cuts at: 2625 2896 3072 3425 4078 4318 5479 5797 6686
Size: 271 176 353 653 240 1161 318 889
Cuts at: 6686 8315 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1161 889 653 443 353 318
275 271 240 187 176 165 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4078 5797 1537
Size: 275 2266 1719 4090
Fragments arranged by size:
4090 2266 1719 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 4665 6280 1353
Size: 1062 2250 1615 3423
Fragments arranged by size:
3423 2250 1615 1062
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6937 7311 7457 387
Size: 1064 138 5348 374 146 1280
Fragments arranged by size:
5348 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2265 2885 3170 3201 3254 3255
Size: 393 436 197 620 285 31 53 1
Cuts at: 3255 3566 4525 4665 5025 5268 6413 7010 7797
Size: 311 959 140 360 243 1145 597 787
Cuts at: 7797 8065 1239
Size: 268 1524
Fragments arranged by size:
1524 1145 959 787 620 597 436 393
360 311 285 268 243 197 140 53
31 1
EcoNI CCTnn'n_nnAGG
Cuts at: 3773 4721 5376 3773
Size: 948 655 6747
Fragments arranged by size:
6747 948 655
EcoO109I rG'GnC_Cy
Cuts at: 2327 2371 2743 2803 3055 3389 4125 4449 4450
Size: 44 372 60 252 334 736 324 1
Cuts at: 4450 4535 5166 5196 5212 5293 2327
Size: 85 631 30 16 81 5384
Fragments arranged by size:
5384 736 631 372 334 324 252 85
81 60 44 30 16 1
EcoRI G'AATT_C
Cuts at: 2291 5659 6037 2291
Size: 3368 378 4604
Fragments arranged by size:
4604 3368 378
EcoRV GAT'ATC
Cuts at: 5136 5136
Size: 8350
FspI TGC'GCA
Cuts at: 6856 6856
Size: 8350
HaeII r_GCGC'y
Cuts at: 1678 3571 3759 7497 1678
Size: 1893 188 3738 2531
Fragments arranged by size:
3738 2531 1893 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2901 2933 3130 3162 4718
Size: 32 135 32 1329 32 197 32 1556
Cuts at: 4718 4750 5050 5082 5143 5175 6991 7023 7065
Size: 32 300 32 61 32 1816 32 42
Cuts at: 7065 7097 1373
Size: 32 2626
Fragments arranged by size:
2626 1816 1556 1329 300 197 135 61
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4108 5694 5940 669
Size: 988 281 2170 1586 246 3079
Fragments arranged by size:
3079 2170 1586 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8350
HpaI GTT'AAC
Cuts at: 5940 5940
Size: 8350
KpnI G_GTAC'C
Cuts at: 3515 5675 3515
Size: 2160 6190
MfeI C'AATT_G
Cuts at: 5949 5949
Size: 8350
MluI A'CGCG_T
Cuts at: 8226 8226
Size: 8350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 3720 5515 7464 7648 1305
Size: 948 1467 1795 1949 184 2007
Fragments arranged by size:
2007 1949 1795 1467 948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 3427 5481 10
Size: 54 1425 343 795 800 2054 2879
Fragments arranged by size:
2879 2054 1425 800 795 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3963 4023 6308 6667 6826 518
Size: 648 1755 1042 60 2285 359 159 2042
Fragments arranged by size:
2285 2042 1755 1042 648 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 3016 4220 4249 4706 5246 5518 5766 6443
Size: 1410 1204 29 457 540 272 248 677
Cuts at: 6443 7591 7836 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1410 1204 1148 677 540 457 272
248 245 29
NcoI C'CATG_G
Cuts at: 513 1065 4047 4374 4998 5497 513
Size: 552 2982 327 624 499 3366
Fragments arranged by size:
3366 2982 624 552 499 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8350
NotI GC'GGCC_GC
Cuts at: 5797 5797
Size: 8350
NspI r_CATG'y
Cuts at: 1132 1229 2686 3923 3982 5488 5708 7253 1132
Size: 97 1457 1237 59 1506 220 1545 2229
Fragments arranged by size:
2229 1545 1506 1457 1237 220 97 59
PasI CC'CwG_GG
Cuts at: 1348 3393 1348
Size: 2045 6305
PciI A'CATG_T
Cuts at: 3978 7249 3978
Size: 3271 5079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2191 5353 1108
Size: 1083 3162 4105
Fragments arranged by size:
4105 3162 1083
PfoI T'CCnGG_A
Cuts at: 2319 2364 2641 2319
Size: 45 277 8028
Fragments arranged by size:
8028 277 45
PmeI GTTT'AAAC
Cuts at: 5654 5654
Size: 8350
PmlI CAC'GTG
Cuts at: 2695 2695
Size: 8350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2531 2563 6419 6451 7932 7964 2531
Size: 32 3856 32 1481 32 2917
Fragments arranged by size:
3856 2917 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2743 2803 3055 3389 4535 5166 2743
Size: 60 252 334 1146 631 5927
Fragments arranged by size:
5927 1146 631 334 252 60
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8350
PsiI TTA'TAA
Cuts at: 5920 5920
Size: 8350
PspOMI G'GGCC_C
Cuts at: 4124 4449 4124
Size: 325 8025
PspXI vC'TCGA_Gb
Cuts at: 2999 5077 2999
Size: 2078 6272
PstI C_TGCA'G
Cuts at: 830 5377 5702 830
Size: 4547 325 3478
Fragments arranged by size:
4547 3478 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8350
PvuII CAG'CTG
Cuts at: 3016 4220 4706 5246 3016
Size: 1204 486 540 6120
Fragments arranged by size:
6120 1204 540 486
SalI G'TCGA_C
Cuts at: 1936 5692 1936
Size: 3756 4594
SanDI GG'GwC_CC
Cuts at: 3055 3055
Size: 8350
SapI GCTCTTCn'nnn_
Cuts at: 2415 2415
Size: 8350
SbfI CC_TGCA'GG
Cuts at: 5702 5702
Size: 8350
ScaI AGT'ACT
Cuts at: 6598 8310 6598
Size: 1712 6638
SexAI A'CCwGG_T
Cuts at: 2934 3241 2934
Size: 307 8043
SfcI C'TryA_G
Cuts at: 826 1045 3434 4956 5373 5698 6833 7514 7705
Size: 219 2389 1522 417 325 1135 681 191
Cuts at: 7705 826
Size: 1471
Fragments arranged by size:
2389 1522 1471 1135 681 417 325 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8350
SmaI CCC'GGG
Cuts at: 1872 3674 5677 1872
Size: 1802 2003 4545
Fragments arranged by size:
4545 2003 1802
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 2999 3296 5077 6454 7355
Size: 197 926 294 762 297 1781 1377 901
Cuts at: 7355 7617 820
Size: 262 1553
Fragments arranged by size:
1781 1553 1377 926 901 762 297 294
262 197
SnaBI TAC'GTA
Cuts at: 493 6141 493
Size: 5648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8350
SphI G_CATG'C
Cuts at: 1132 3923 5708 1132
Size: 2791 1785 3774
Fragments arranged by size:
3774 2791 1785
SspI AAT'ATT
Cuts at: 5 52 2107 6274 5
Size: 47 2055 4167 2081
Fragments arranged by size:
4167 2081 2055 47
StuI AGG'CCT
Cuts at: 1517 2638 2870 5382 5567 1517
Size: 1121 232 2512 185 4300
Fragments arranged by size:
4300 2512 1121 232 185
StyI C'CwwG_G
Cuts at: 513 1065 2899 2905 2927 3060 4047 4374 4399
Size: 552 1834 6 22 133 987 327 25
Cuts at: 4399 4586 4998 5169 5497 5789 513
Size: 187 412 171 328 292 3074
Fragments arranged by size:
3074 1834 987 552 412 328 327 292
187 171 133 25 22 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4124 4307 5422 5658 6377 6394 6547
Size: 1255 2544 183 1115 236 719 17 153
Cuts at: 6547 6732 7177 325
Size: 185 445 1498
Fragments arranged by size:
2544 1498 1255 1115 719 445 236 185
183 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 5144 6596
Size: 274 80 33 51 1443 498 2669 1452
Cuts at: 6596 8308 96
Size: 1712 138
Fragments arranged by size:
2669 1712 1452 1443 498 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8310 8342 8310
Size: 32 8318
Tth111I GACn'n_nGTC
Cuts at: 1316 5026 1316
Size: 3710 4640
XbaI T'CTAG_A
Cuts at: 5686 5686
Size: 8350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3067 3420 3911 3962 4549 5504 5621 3067
Size: 353 491 51 587 955 117 5796
Fragments arranged by size:
5796 955 587 491 353 117 51
XhoI C'TCGA_G
Cuts at: 1943 2999 5077 1943
Size: 1056 2078 5216
Fragments arranged by size:
5216 2078 1056
XmaI C'CCGG_G
Cuts at: 1870 3672 5675 1870
Size: 1802 2003 4545
Fragments arranged by size:
4545 2003 1802
XmnI GAAnn'nnTTC
Cuts at: 6479 6479
Size: 8350
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6158 276
Size: 53 83 186 936 4624 2468
Fragments arranged by size:
4624 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PsrI RsrII SacI SacII
SfiI SfoI SrfI SwaI