Restriction map for
pFN21AA0898
(Circular) (Six-base) MAPSORT of: pFN21AA0898.seq Check: 8452 from: 1 to: 7597
pFN21AA0898 7597 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3937 3937
Size: 7597
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5407 278
Size: 53 83 186 936 3871 2468
Fragments arranged by size:
3871 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4940 1937
Size: 3003 4594
Acc65I G'GTAC_C
Cuts at: 4918 4918
Size: 7597
AclI AA'CG_TT
Cuts at: 1563 5724 6097 1563
Size: 4161 373 3063
Fragments arranged by size:
4161 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 2654 4407 4500 4665 4692 4713 4878
Size: 983 39 1753 93 165 27 21 165
Cuts at: 4878 5660 7044 7312 1632
Size: 782 1384 268 1917
Fragments arranged by size:
1917 1753 1384 983 782 268 165 165
93 39 27 21
AflII C'TTAA_G
Cuts at: 820 1017 3878 820
Size: 197 2861 4539
Fragments arranged by size:
4539 2861 197
AflIII A'CryG_T
Cuts at: 1203 2419 6496 7473 1203
Size: 1216 4077 977 1327
Fragments arranged by size:
4077 1327 1216 977
AgeI A'CCGG_T
Cuts at: 6428 6428
Size: 7597
AhdI GACnn_n'nnGTC
Cuts at: 6326 6326
Size: 7597
AleI CACnn'nnGTG
Cuts at: 1166 2083 2429 1166
Size: 917 346 6334
Fragments arranged by size:
6334 917 346
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7597
AlwNI CAG_nnn'CTG
Cuts at: 2888 3473 3743 4381 4514 5340 6912 2888
Size: 585 270 638 133 826 1572 3573
Fragments arranged by size:
3573 1572 826 638 585 270 133
ApaLI G'TGCA_C
Cuts at: 2355 5654 6810 2355
Size: 3299 1156 3142
Fragments arranged by size:
3299 3142 1156
ApoI r'AATT_y
Cuts at: 1493 2406 2857 3089 3901 4906 5132 5284 7393
Size: 913 451 232 812 1005 226 152 2109
Cuts at: 7393 1493
Size: 1697
Fragments arranged by size:
2109 1697 1005 913 812 451 232 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3854 6151 160
Size: 872 2822 2297 1606
Fragments arranged by size:
2822 2297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7597
AvaI C'yCGr_G
Cuts at: 1870 1943 3240 4434 4922 1870
Size: 73 1297 1194 488 4545
Fragments arranged by size:
4545 1297 1194 488 73
BamHI G'GATC_C
Cuts at: 4927 5294 4927
Size: 367 7230
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4918 6373 618
Size: 325 217 627 3131 1455 1842
Fragments arranged by size:
3131 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2152 2729 2997 4916 1391
Size: 761 577 268 1919 4072
Fragments arranged by size:
4072 1919 761 577 268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2633 4488 928
Size: 971 734 1855 4037
Fragments arranged by size:
4037 1855 971 734
BbvCI CC'TCA_GC
Cuts at: 2034 2034
Size: 7597
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2741 2775 5788 5822 1903
Size: 34 804 34 3013 34 3678
Fragments arranged by size:
3678 3013 804 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 5489 6699 3161
Size: 2328 1210 4059
Fragments arranged by size:
4059 2328 1210
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 6670
BfrBI ATG'CAT
Cuts at: 2374 2374
Size: 7597
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6208 136
Size: 107 122 71 631 605 4536 1525
Fragments arranged by size:
4536 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3553 4861 7592 3553
Size: 1308 2731 3558
Fragments arranged by size:
3558 2731 1308
BlpI GC'TnA_GC
Cuts at: 5014 5014
Size: 7597
Bme1580I G_kGCm'C
Cuts at: 1790 2359 4011 5658 6814 1790
Size: 569 1652 1647 1156 2573
Fragments arranged by size:
2573 1652 1647 1156 569
BmgBI CAC'GTC
Cuts at: 1318 2422 1318
Size: 1104 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5328 6286 442
Size: 416 53 83 399 24 3911 958 1753
Fragments arranged by size:
3911 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2947 3011 3428 3696 4143 4365 4668
Size: 923 785 64 417 268 447 222 303
Cuts at: 4668 6257 1239
Size: 1589 2579
Fragments arranged by size:
2579 1589 923 785 447 417 303 268
222 64
Bpu10I CC'TnA_GC
Cuts at: 2034 2034
Size: 7597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5722 6587 6885 5722
Size: 865 298 6434
Fragments arranged by size:
6434 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6260 882
Size: 617 4761 2219
Fragments arranged by size:
4761 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5388 493
Size: 711 4184 2702
Fragments arranged by size:
4184 2702 711
BsaBI GATnn'nnATC
Cuts at: 3378 3531 3552 3558 3564 7591 3378
Size: 153 21 6 6 4027 3384
Fragments arranged by size:
4027 3384 153 21 6 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2461 5404 5786 6421
Size: 53 83 186 936 928 2943 382 635
Cuts at: 6421 275
Size: 1451
Fragments arranged by size:
2943 1451 936 928 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3885 6030 6428 6702 6849 7260 3885
Size: 2145 398 274 147 411 4222
Fragments arranged by size:
4222 2145 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 6073
Fragments arranged by size:
6073 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3521 4757 1186
Size: 2335 1236 4026
Fragments arranged by size:
4026 2335 1236
BseYI C'CCAG_C
Cuts at: 3120 6800 7296 3120
Size: 3680 496 3421
Fragments arranged by size:
3680 3421 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3261 1163
Size: 573 1525 5499
Fragments arranged by size:
5499 1525 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5047 5808 6836 1540
Size: 275 186 3046 761 1028 2301
Fragments arranged by size:
3046 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2152 2173 2359 2685 2729 2997 5658 5743 6814
Size: 21 186 326 44 268 2661 85 1071
Cuts at: 6814 2152
Size: 2935
Fragments arranged by size:
2935 2661 1071 326 268 186 85 44
21
BsmI GAATG_Cn'
Cuts at: 2376 2560 3122 5108 5201 2376
Size: 184 562 1986 93 4772
Fragments arranged by size:
4772 1986 562 184 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2468 2468
Size: 7597
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2173 2359 2685 2729 2997 4011
Size: 399 362 21 186 326 44 268 1014
Cuts at: 4011 4916 5658 5743 6814 1391
Size: 905 742 85 1071 2174
Fragments arranged by size:
2174 1071 1014 905 742 399 362 326
268 186 85 44 21
BspEI T'CCGG_A
Cuts at: 7260 7260
Size: 7597
BspHI T'CATG_A
Cuts at: 4794 5486 7216 4794
Size: 692 1730 5175
Fragments arranged by size:
5175 1730 692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3746 3937 4301 4952 844
Size: 2902 191 364 651 3489
Fragments arranged by size:
3489 2902 651 364 191
BsrBI CCG'CTC
Cuts at: 1394 1823 5484 1394
Size: 429 3661 3507
Fragments arranged by size:
3661 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2028 2092 2161 4002 6092 6266 65
Size: 1183 780 64 69 1841 2090 174 1396
Fragments arranged by size:
2090 1841 1396 1183 780 174 69 64
BsrFI r'CCGG_y
Cuts at: 1700 1810 4485 6241 6428 1700
Size: 110 2675 1756 187 2869
Fragments arranged by size:
2869 2675 1756 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7597
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7597
BssSI C'ACGA_G
Cuts at: 3414 3536 5657 6669 3414
Size: 122 2121 1012 4342
Fragments arranged by size:
4342 2121 1012 122
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 3743 794
Size: 1895 1054 4648
Fragments arranged by size:
4648 1895 1054
BstBI TT'CG_AA
Cuts at: 4327 6401 4327
Size: 2074 5523
BstEII G'GTnAC_C
Cuts at: 1184 4570 1184
Size: 3386 4211
BstYI r'GATC_y
Cuts at: 1972 2733 2884 3553 3559 3565 4861 4927 5294
Size: 761 151 669 6 6 1296 66 367
Cuts at: 5294 5679 5696 7148 7234 7246 7592 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1296 761 669 385 367 346
151 86 66 17 12 6 6
BtgI C'CryG_G
Cuts at: 513 1065 2004 2769 3766 513
Size: 552 939 765 997 4344
Fragments arranged by size:
4344 997 939 765 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6858
Fragments arranged by size:
6858 683 56
BtsI GCAGTG_nn'
Cuts at: 2565 4117 4341 4496 5113 5907 5927 2565
Size: 1552 224 155 617 794 20 4235
Fragments arranged by size:
4235 1552 794 617 224 155 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7597
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7562
DraI TTT'AAA
Cuts at: 2392 4020 4901 2392
Size: 1628 881 5088
Fragments arranged by size:
5088 1628 881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6604 809
Size: 507 5288 1802
Fragments arranged by size:
5288 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4050 5044 5933
Size: 54 1425 50 275 18 2220 994 889
Cuts at: 5933 7562 8
Size: 1629 43
Fragments arranged by size:
2220 1629 1425 994 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5044 1537
Size: 275 3232 4090
Fragments arranged by size:
4090 3232 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2244 2433 2910 3708 4268 4324 4746 5527
Size: 891 189 477 798 560 56 422 781
Cuts at: 5527 1353
Size: 3423
Fragments arranged by size:
3423 891 798 781 560 477 422 189
56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2394 6184 6558 6704 387
Size: 1064 138 805 3790 374 146 1280
Fragments arranged by size:
3790 1280 1064 805 374 146 138
EcoICRI GAG'CTC
Cuts at: 2150 2727 2995 2150
Size: 577 268 6752
Fragments arranged by size:
6752 577 268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2615 2654 2947 3011 3428 3696
Size: 393 530 453 39 293 64 417 268
Cuts at: 3696 4143 4365 4407 4500 4665 4668 4692 4713
Size: 447 222 42 93 165 3 24 21
Cuts at: 4713 4878 5660 6257 7044 7312 1239
Size: 165 782 597 787 268 1524
Fragments arranged by size:
1524 787 782 597 530 453 447 417
393 293 268 268 222 165 165 93
64 42 39 24 21 3
EcoNI CCTnn'n_nnAGG
Cuts at: 4695 4695
Size: 7597
EcoO109I rG'GnC_Cy
Cuts at: 3749 4853 3749
Size: 1104 6493
EcoRI G'AATT_C
Cuts at: 4906 5284 4906
Size: 378 7219
EcoRV GAT'ATC
Cuts at: 2718 2718
Size: 7597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3338 3338
Size: 7597
FspI TGC'GCA
Cuts at: 6103 6103
Size: 7597
HaeII r_GCGC'y
Cuts at: 1678 2131 6744 1678
Size: 453 4613 2531
Fragments arranged by size:
4613 2531 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2702 2734 2869 2901 6238
Size: 32 135 32 1130 32 135 32 3337
Cuts at: 6238 6270 6312 6344 1373
Size: 32 42 32 2626
Fragments arranged by size:
3337 2626 1130 135 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4941 5187 669
Size: 988 281 3003 246 3079
Fragments arranged by size:
3079 3003 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2603 4497 748
Size: 1855 1894 3848
Fragments arranged by size:
3848 1894 1855
HpaI GTT'AAC
Cuts at: 5187 5187
Size: 7597
KpnI G_GTAC'C
Cuts at: 4922 4922
Size: 7597
MfeI C'AATT_G
Cuts at: 4243 5196 4243
Size: 953 6644
MluI A'CGCG_T
Cuts at: 7473 7473
Size: 7597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3091 3249 3319 3852 6711 6895 1305
Size: 1786 158 70 533 2859 184 2007
Fragments arranged by size:
2859 2007 1786 533 184 158 70
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5775
Fragments arranged by size:
5775 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2083 2166 2352 2429 3771 3811
Size: 648 891 26 83 186 77 1342 40
Cuts at: 3811 4761 4793 5555 5914 6073 518
Size: 950 32 762 359 159 2042
Fragments arranged by size:
2042 1342 950 891 762 648 359 186
159 83 77 40 32 26
MspA1I CmG'CkG
Cuts at: 1606 2118 2593 3002 3740 3821 4685 5013 5690
Size: 512 475 409 738 81 864 328 677
Cuts at: 5690 6838 7083 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 864 738 677 512 475 409
328 245 81
NcoI C'CATG_G
Cuts at: 513 1065 2004 2769 3766 513
Size: 552 939 765 997 4344
Fragments arranged by size:
4344 997 939 765 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7597
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7597
NotI GC'GGCC_GC
Cuts at: 5044 5044
Size: 7597
NsiI A_TGCA'T
Cuts at: 2376 2376
Size: 7597
NspI r_CATG'y
Cuts at: 1132 1229 3435 3445 4132 4176 4955 6500 1132
Size: 97 2206 10 687 44 779 1545 2229
Fragments arranged by size:
2229 2206 1545 779 687 97 44 10
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7597
PciI A'CATG_T
Cuts at: 6496 6496
Size: 7597
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7597
PmeI GTTT'AAAC
Cuts at: 4901 4901
Size: 7597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5666 5698 7179 7211 5666
Size: 32 1481 32 6052
Fragments arranged by size:
6052 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3749 3749
Size: 7597
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7597
PsiI TTA'TAA
Cuts at: 2609 5167 2609
Size: 2558 5039
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3030 3062 3030
Size: 32 7565
PstI C_TGCA'G
Cuts at: 830 2252 3748 3945 4617 4949 830
Size: 1422 1496 197 672 332 3478
Fragments arranged by size:
3478 1496 1422 672 332 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7597
PvuII CAG'CTG
Cuts at: 2118 2593 3002 3740 4685 2118
Size: 475 409 738 945 5030
Fragments arranged by size:
5030 945 738 475 409
SacI G_AGCT'C
Cuts at: 2152 2729 2997 2152
Size: 577 268 6752
Fragments arranged by size:
6752 577 268
SalI G'TCGA_C
Cuts at: 1936 4939 1936
Size: 3003 4594
SapI GCTCTTCn'nnn_
Cuts at: 4324 4746 4324
Size: 422 7175
SbfI CC_TGCA'GG
Cuts at: 4949 4949
Size: 7597
ScaI AGT'ACT
Cuts at: 5845 7557 5845
Size: 1712 5885
SfcI C'TryA_G
Cuts at: 826 1045 2248 3465 3744 3941 4613 4945 6080
Size: 219 1203 1217 279 197 672 332 1135
Cuts at: 6080 6761 6952 826
Size: 681 191 1471
Fragments arranged by size:
1471 1217 1203 1135 681 672 332 279
219 197 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7597
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7597
SmaI CCC'GGG
Cuts at: 1872 3242 4924 1872
Size: 1370 1682 4545
Fragments arranged by size:
4545 1682 1370
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3878 5701 6602 6864 820
Size: 197 926 1935 1823 901 262 1553
Fragments arranged by size:
1935 1823 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5388 493
Size: 4895 2702
SpeI A'CTAG_T
Cuts at: 152 2191 3284 152
Size: 2039 1093 4465
Fragments arranged by size:
4465 2039 1093
SphI G_CATG'C
Cuts at: 1132 3435 4955 1132
Size: 2303 1520 3774
Fragments arranged by size:
3774 2303 1520
SspI AAT'ATT
Cuts at: 5 52 5521 5
Size: 47 5469 2081
Fragments arranged by size:
5469 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7597
StyI C'CwwG_G
Cuts at: 513 1065 2004 2769 3766 4575 5036 513
Size: 552 939 765 997 809 461 3074
Fragments arranged by size:
3074 997 939 809 765 552 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5624 5641 5794 5979 6424 325
Size: 1255 4044 17 153 185 445 1498
Fragments arranged by size:
4044 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4663 5843 7555
Size: 274 80 33 51 1443 2686 1180 1712
Cuts at: 7555 96
Size: 138
Fragments arranged by size:
2686 1712 1443 1180 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2779 2811 7557 7589 2779
Size: 32 4746 32 2787
Fragments arranged by size:
4746 2787 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4381 1316
Size: 3065 4532
XbaI T'CTAG_A
Cuts at: 4933 4933
Size: 7597
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7597
XmaI C'CCGG_G
Cuts at: 1870 3240 4922 1870
Size: 1370 1682 4545
Fragments arranged by size:
4545 1682 1370
XmnI GAAnn'nnTTC
Cuts at: 5726 5726
Size: 7597
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5405 276
Size: 53 83 186 936 3871 2468
Fragments arranged by size:
3871 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI