Restriction map for
pFN21AA0896
(Circular) (Six-base) MAPSORT of: pFN21AA0896.seq Check: 7350 from: 1 to: 13099
pFN21AA0896 13099 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3461 3461
Size: 13099
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2012 2309 10909 278
Size: 53 83 186 936 476 297 8600 2468
Fragments arranged by size:
8600 2468 936 476 297 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4222 4669 5011 6111 7431 8107 9405 9973
Size: 2285 447 342 1100 1320 676 1298 568
Cuts at: 9973 10442 1937
Size: 469 4594
Fragments arranged by size:
4594 2285 1320 1298 1100 676 568 469
447 342
Acc65I G'GTAC_C
Cuts at: 3596 4454 5489 7271 10420 3596
Size: 858 1035 1782 3149 6275
Fragments arranged by size:
6275 3149 1782 1035 858
AclI AA'CG_TT
Cuts at: 1563 11226 11599 1563
Size: 9663 373 3063
Fragments arranged by size:
9663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2615 2841 3801 4304 5952 7011 7572 8160
Size: 983 226 960 503 1648 1059 561 588
Cuts at: 8160 9162 11162 12546 12814 1632
Size: 1002 2000 1384 268 1917
Fragments arranged by size:
2000 1917 1648 1384 1059 1002 983 960
588 561 503 268 226
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12902
AflIII A'CryG_T
Cuts at: 1203 3851 9658 11998 12975 1203
Size: 2648 5807 2340 977 1327
Fragments arranged by size:
5807 2648 2340 1327 977
AgeI A'CCGG_T
Cuts at: 5168 11930 5168
Size: 6762 6337
AhdI GACnn_n'nnGTC
Cuts at: 10319 11828 10319
Size: 1509 11590
AleI CACnn'nnGTG
Cuts at: 1166 2787 4749 1166
Size: 1621 1962 9516
Fragments arranged by size:
9516 1962 1621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 8199 9720 10318 1184
Size: 7015 1521 598 3965
Fragments arranged by size:
7015 3965 1521 598
AlwNI CAG_nnn'CTG
Cuts at: 2354 2552 4100 4726 6863 6944 7304 7451 8051
Size: 198 1548 626 2137 81 360 147 600
Cuts at: 8051 8627 9140 9773 10842 12414 2354
Size: 576 513 633 1069 1572 3039
Fragments arranged by size:
3039 2137 1572 1548 1069 633 626 600
576 513 360 198 147 81
ApaLI G'TGCA_C
Cuts at: 11156 12312 11156
Size: 1156 11943
ApoI r'AATT_y
Cuts at: 1493 3113 4046 7633 8675 9994 10384 10408 10634
Size: 1620 933 3587 1042 1319 390 24 226
Cuts at: 10634 10786 12895 1493
Size: 152 2109 1697
Fragments arranged by size:
3587 2109 1697 1620 1319 1042 933 390
226 152 24
AseI AT'TA_AT
Cuts at: 160 1032 11653 160
Size: 872 10621 1606
Fragments arranged by size:
10621 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13099
AvaI C'yCGr_G
Cuts at: 1870 1943 2290 2457 2478 2886 10424 1870
Size: 73 347 167 21 408 7538 4545
Fragments arranged by size:
7538 4545 408 347 167 73 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3588 3621 6204 6237 3588
Size: 33 2583 33 10450
Fragments arranged by size:
10450 2583 33 33
BamHI G'GATC_C
Cuts at: 2429 4498 5047 5104 10429 10796 2429
Size: 2069 549 57 5325 367 4732
Fragments arranged by size:
5325 4732 2069 549 367 57
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3596 4028 4454 5489
Size: 325 217 627 255 1554 432 426 1035
Cuts at: 5489 7271 8571 8966 10420 11875 618
Size: 1782 1300 395 1454 1455 1842
Fragments arranged by size:
1842 1782 1554 1455 1454 1300 1035 627
432 426 395 325 255 217
BanII G_rGCy'C
Cuts at: 1391 2458 3133 10418 1391
Size: 1067 675 7285 4072
Fragments arranged by size:
7285 4072 1067 675
BbeI G_GCGC'C
Cuts at: 8575 8575
Size: 13099
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 4292 6831 9106 928
Size: 971 930 1463 2539 2275 4921
Fragments arranged by size:
4921 2539 2275 1463 971 930
BbvCI CC'TCA_GC
Cuts at: 6792 6792
Size: 13099
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 11290 11324 1903
Size: 34 9353 34 3678
Fragments arranged by size:
9353 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7900 8957 9395 10991 12201 7900
Size: 1057 438 1596 1210 8798
Fragments arranged by size:
8798 1596 1210 1057 438
BclI T'GATC_A
Cuts at: 1548 6580 8158 10071 1548
Size: 5032 1578 1913 4576
Fragments arranged by size:
5032 4576 1913 1578
BfrBI ATG'CAT
Cuts at: 3863 3863
Size: 13099
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11710 136
Size: 107 122 71 631 605 10038 1525
Fragments arranged by size:
10038 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3734 8749 10183 13094 3734
Size: 5015 1434 2911 3739
Fragments arranged by size:
5015 3739 2911 1434
BlpI GC'TnA_GC
Cuts at: 3459 3786 7715 9282 10516 3459
Size: 327 3929 1567 1234 6042
Fragments arranged by size:
6042 3929 1567 1234 327
Bme1580I G_kGCm'C
Cuts at: 1790 2045 3445 11160 12316 1790
Size: 255 1400 7715 1156 2573
Fragments arranged by size:
7715 2573 1400 1156 255
BmgBI CAC'GTC
Cuts at: 1318 7703 1318
Size: 6385 6714
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7961 10830 11788
Size: 416 53 83 399 24 6544 2869 958
Cuts at: 11788 442
Size: 1753
Fragments arranged by size:
6544 2869 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3771 7320 7677 1056
Size: 2715 3549 357 6478
Fragments arranged by size:
6478 3549 2715 357
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2932 5843 8771 2932
Size: 2911 2928 7260
Fragments arranged by size:
7260 2928 2911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 2427 2472 2757 3657 3825 4045 5043
Size: 1180 8 45 285 900 168 220 998
Cuts at: 5043 5282 5577 5630 6580 6762 11759 1239
Size: 239 295 53 950 182 4997 2579
Fragments arranged by size:
4997 2579 1180 998 950 900 295 285
239 220 182 168 53 45 8
Bpu10I CC'TnA_GC
Cuts at: 6792 9165 6792
Size: 2373 10726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 3631 4709 5425 7000 11224 12089 12387 2210
Size: 1421 1078 716 1575 4224 865 298 2922
Fragments arranged by size:
4224 2922 1575 1421 1078 865 716 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3963 7058 7788 9006 11762 882
Size: 617 2464 3095 730 1218 2756 2219
Fragments arranged by size:
3095 2756 2464 2219 1218 730 617
BsaAI yAC'GTr
Cuts at: 493 1204 2957 3852 10890 493
Size: 711 1753 895 7038 2702
Fragments arranged by size:
7038 2702 1753 895 711
BsaBI GATnn'nnATC
Cuts at: 4758 8742 8748 13093 4758
Size: 3984 6 4345 4764
Fragments arranged by size:
4764 4345 3984 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2306 8572 10906
Size: 53 83 186 936 476 297 6266 2334
Cuts at: 10906 11288 11923 275
Size: 382 635 1451
Fragments arranged by size:
6266 2334 1451 936 635 476 382 297
186 83 53
BsaWI w'CCGG_w
Cuts at: 5168 11532 11930 12204 12351 12762 5168
Size: 6364 398 274 147 411 5505
Fragments arranged by size:
6364 5505 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5603 5633 9037 9040 9067
Size: 30 165 30 4200 30 3404 3 27
Cuts at: 9067 9070 9828 9858 1178
Size: 3 758 30 4419
Fragments arranged by size:
4419 4200 3404 758 165 30 30 30
30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2579 7761 9018 9058 9061 9400 1186
Size: 1393 5182 1257 40 3 339 4885
Fragments arranged by size:
5182 4885 1393 1257 339 40 3
BseYI C'CCAG_C
Cuts at: 2532 3662 5979 7167 9277 12302 12798 2532
Size: 1130 2317 1188 2110 3025 496 2833
Fragments arranged by size:
3025 2833 2317 2110 1188 1130 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3903 6471 7725 8424 1163
Size: 573 2167 2568 1254 699 5838
Fragments arranged by size:
5838 2568 2167 1254 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 10549 11310 12338 1540
Size: 275 186 8548 761 1028 2301
Fragments arranged by size:
8548 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2458 3384 5568 5826 11160 11245 12316 2458
Size: 926 2184 258 5334 85 1071 3241
Fragments arranged by size:
5334 3241 2184 1071 926 258 85
BsmI GAATG_Cn'
Cuts at: 4549 5260 6019 7798 10610 10703 4549
Size: 711 759 1779 2812 93 6945
Fragments arranged by size:
6945 2812 1779 759 711 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4975 9762 4975
Size: 4787 8312
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2458 3133 3384 3445 5568 5826
Size: 399 255 413 675 251 61 2123 258
Cuts at: 5826 10418 11160 11245 12316 1391
Size: 4592 742 85 1071 2174
Fragments arranged by size:
4592 2174 2123 1071 742 675 413 399
258 255 251 85 61
BspEI T'CCGG_A
Cuts at: 12762 12762
Size: 13099
BspHI T'CATG_A
Cuts at: 5659 5953 6757 8428 10988 12718 5659
Size: 294 804 1671 2560 1730 6040
Fragments arranged by size:
6040 2560 1730 1671 804 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 4144 9056 10454 844
Size: 2617 683 4912 1398 3489
Fragments arranged by size:
4912 3489 2617 1398 683
BsrBI CCG'CTC
Cuts at: 1394 1823 3418 10986 1394
Size: 429 1595 7568 3507
Fragments arranged by size:
7568 3507 1595 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3433 4368 4887 6157 6401 7890 8898
Size: 1183 2185 935 519 1270 244 1489 1008
Cuts at: 8898 9231 9798 9966 11594 11768 65
Size: 333 567 168 1628 174 1396
Fragments arranged by size:
2185 1628 1489 1396 1270 1183 1008 935
567 519 333 244 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 2469 5168 8352 11743 11930 1700
Size: 110 659 2699 3184 3391 187 2869
Fragments arranged by size:
3391 3184 2869 2699 659 187 110
BsrGI T'GTAC_A
Cuts at: 96 6176 8824 9217 96
Size: 6080 2648 393 3978
Fragments arranged by size:
6080 3978 2648 393
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13099
BssSI C'ACGA_G
Cuts at: 3240 7743 9213 11159 12171 3240
Size: 4503 1470 1946 1012 4168
Fragments arranged by size:
4503 4168 1946 1470 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6944 7304 794
Size: 6150 360 6589
Fragments arranged by size:
6589 6150 360
BstBI TT'CG_AA
Cuts at: 11903 11903
Size: 13099
BstEII G'GTnAC_C
Cuts at: 1184 2840 1184
Size: 1656 11443
BstXI CCAn_nnnn'nTGG
Cuts at: 7118 8152 7118
Size: 1034 12065
BstYI r'GATC_y
Cuts at: 1972 2429 2743 3734 4498 4513 5047 5104 5147
Size: 457 314 991 764 15 534 57 43
Cuts at: 5147 8197 8749 9391 10183 10429 10796 11181 11198
Size: 3050 552 642 792 246 367 385 17
Cuts at: 11198 12650 12736 12748 13094 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
3050 1977 1452 991 792 764 642 552
534 457 385 367 346 314 246 86
57 43 17 15 12
BstZ17I GTA'TAC
Cuts at: 4223 6112 7432 8108 4223
Size: 1889 1320 676 9214
Fragments arranged by size:
9214 1889 1320 676
Bsu36I CC'TnA_GG
Cuts at: 2386 2386
Size: 13099
BtgI C'CryG_G
Cuts at: 513 1065 2336 9339 9442 513
Size: 552 1271 7003 103 4170
Fragments arranged by size:
7003 4170 1271 552 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5114 9171 487
Size: 683 56 3888 4057 4415
Fragments arranged by size:
4415 4057 3888 683 56
BtsI GCAGTG_nn'
Cuts at: 3092 3384 3886 3959 4254 4410 5781 6538 6933
Size: 292 502 73 295 156 1371 757 395
Cuts at: 6933 6976 7154 8875 10214 10615 11409 11429 3092
Size: 43 178 1721 1339 401 794 20 4762
Fragments arranged by size:
4762 1721 1371 1339 794 757 502 401
395 295 292 178 156 73 43 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4150 4185 8154 8189 529
Size: 35 3586 35 3969 35 5439
Fragments arranged by size:
5439 3969 3586 35 35 35
DraI TTT'AAA
Cuts at: 2106 10403 2106
Size: 8297 4802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 12106 809
Size: 507 10790 1802
Fragments arranged by size:
10790 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4470 5371 8053
Size: 54 1425 50 275 18 2640 901 2682
Cuts at: 8053 8206 10546 11435 13064 8
Size: 153 2340 889 1629 43
Fragments arranged by size:
2682 2640 2340 1629 1425 901 889 275
153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10546 1537
Size: 275 8734 4090
Fragments arranged by size:
8734 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3924 3942 3966 4821 6096 6708 6741 7623
Size: 2571 18 24 855 1275 612 33 882
Cuts at: 7623 7639 8043 11029 1353
Size: 16 404 2986 3423
Fragments arranged by size:
3423 2986 2571 1275 882 855 612 404
33 24 18 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11686 12060 12206 387
Size: 1064 138 10097 374 146 1280
Fragments arranged by size:
10097 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2456 2456
Size: 13099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 2427 2472 2615 2757 2841 3657
Size: 393 787 8 45 143 142 84 816
Cuts at: 3657 3801 3825 4045 4304 5043 5282 5577 5630
Size: 144 24 220 259 739 239 295 53
Cuts at: 5630 5952 6580 6762 7011 7572 8160 9162 11162
Size: 322 628 182 249 561 588 1002 2000
Cuts at: 11162 11759 12546 12814 1239
Size: 597 787 268 1524
Fragments arranged by size:
2000 1524 1002 816 787 787 739 628
597 588 561 393 322 295 268 259
249 239 220 182 144 143 142 84
53 45 24 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2437 2437
Size: 13099
EcoO109I rG'GnC_Cy
Cuts at: 3827 5207 9123 3827
Size: 1380 3916 7803
Fragments arranged by size:
7803 3916 1380
EcoRI G'AATT_C
Cuts at: 7633 10408 10786 7633
Size: 2775 378 9946
Fragments arranged by size:
9946 2775 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3188 6080 7523 8028 9692 3188
Size: 2892 1443 505 1664 6595
Fragments arranged by size:
6595 2892 1664 1443 505
FspI TGC'GCA
Cuts at: 11605 11605
Size: 13099
HaeII r_GCGC'y
Cuts at: 1678 7722 8575 9442 12246 1678
Size: 6044 853 867 2804 2531
Fragments arranged by size:
6044 2804 2531 867 853
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2492 2524 2626 2658 2735
Size: 32 135 32 920 32 102 32 77
Cuts at: 2735 2767 2966 2998 3604 3636 3851 3883 3941
Size: 32 199 32 606 32 215 32 58
Cuts at: 3941 3973 4296 4328 6463 6495 6989 7021 7814
Size: 32 323 32 2135 32 494 32 793
Cuts at: 7814 7846 8018 8050 8823 8855 9193 9225 9955
Size: 32 172 32 773 32 338 32 730
Cuts at: 9955 9987 10305 10337 11740 11772 11814 11846 1373
Size: 32 318 32 1403 32 42 32 2626
Fragments arranged by size:
2626 2135 1403 920 793 773 730 606
494 338 323 318 215 199 172 135
102 77 58 42 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3261 3912 4670 10443 10689 669
Size: 988 281 1323 651 758 5773 246 3079
Fragments arranged by size:
5773 3079 1323 988 758 651 281 246
HindIII A'AGCT_T
Cuts at: 748 3745 4214 7505 10118 748
Size: 2997 469 3291 2613 3729
Fragments arranged by size:
3729 3291 2997 2613 469
HpaI GTT'AAC
Cuts at: 3261 10689 3261
Size: 7428 5671
KasI G'GCGC_C
Cuts at: 8571 8571
Size: 13099
KpnI G_GTAC'C
Cuts at: 3600 4458 5493 7275 10424 3600
Size: 858 1035 1782 3149 6275
Fragments arranged by size:
6275 3149 1782 1035 858
MfeI C'AATT_G
Cuts at: 3870 6372 9691 9810 10698 3870
Size: 2502 3319 119 888 6271
Fragments arranged by size:
6271 3319 2502 888 119
MluI A'CGCG_T
Cuts at: 12975 12975
Size: 13099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4823 6506 8098 8324 8596 9246 12213 12397
Size: 3518 1683 1592 226 272 650 2967 184
Cuts at: 12397 1305
Size: 2007
Fragments arranged by size:
3518 2967 2007 1683 1592 650 272 226
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4472 8055 10
Size: 54 1425 343 2640 3583 5054
Fragments arranged by size:
5054 3583 2640 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2787 3438 3850 4749 4779 5471 6759
Size: 648 1621 651 412 899 30 692 1288
Cuts at: 6759 7116 7791 7803 7983 9257 11057 11416 11575
Size: 357 675 12 180 1274 1800 359 159
Cuts at: 11575 518
Size: 2042
Fragments arranged by size:
2042 1800 1621 1288 1274 899 692 675
651 648 412 359 357 180 159 30
12
MspA1I CmG'CkG
Cuts at: 1606 2035 2397 2450 3463 3717 5000 5141 5363
Size: 429 362 53 1013 254 1283 141 222
Cuts at: 5363 6863 7229 7295 7304 7451 9286 10515 11192
Size: 1500 366 66 9 147 1835 1229 677
Cuts at: 11192 12340 12585 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1835 1500 1283 1229 1148 1013 677
429 366 362 254 245 222 147 141
66 53 9
NaeI GCC'GGC
Cuts at: 2471 2471
Size: 13099
NarI GG'CG_CC
Cuts at: 8572 8572
Size: 13099
NcoI C'CATG_G
Cuts at: 513 1065 2336 9339 9442 513
Size: 552 1271 7003 103 4170
Fragments arranged by size:
7003 4170 1271 552 103
NdeI CA'TA_TG
Cuts at: 387 7849 387
Size: 7462 5637
NgoMIV G'CCGG_C
Cuts at: 2469 2469
Size: 13099
NheI G'CTAG_C
Cuts at: 1052 3767 7316 7673 1052
Size: 2715 3549 357 6478
Fragments arranged by size:
6478 3549 2715 357
NotI GC'GGCC_GC
Cuts at: 10546 10546
Size: 13099
NsiI A_TGCA'T
Cuts at: 3865 3865
Size: 13099
NspI r_CATG'y
Cuts at: 1132 1229 4778 7844 8347 9262 9662 9959 10457
Size: 97 3549 3066 503 915 400 297 498
Cuts at: 10457 12002 1132
Size: 1545 2229
Fragments arranged by size:
3549 3066 2229 1545 915 503 498 400
297 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 13099
PciI A'CATG_T
Cuts at: 9658 11998 9658
Size: 2340 10759
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 9554 1108
Size: 915 7531 4653
Fragments arranged by size:
7531 4653 915
PfoI T'CCnGG_A
Cuts at: 4051 7597 9387 4051
Size: 3546 1790 7763
Fragments arranged by size:
7763 3546 1790
PmeI GTTT'AAAC
Cuts at: 10403 10403
Size: 13099
PmlI CAC'GTG
Cuts at: 3852 3852
Size: 13099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2273 2305 11168 11200 12681 12713 2273
Size: 32 8863 32 1481 32 2659
Fragments arranged by size:
8863 2659 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3827 5207 3827
Size: 1380 11719
PshAI GACnn'nnGTC
Cuts at: 1613 3907 1613
Size: 2294 10805
PsiI TTA'TAA
Cuts at: 10669 10669
Size: 13099
PspXI vC'TCGA_Gb
Cuts at: 2478 2478
Size: 13099
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 9936 9968 9936
Size: 32 13067
PstI C_TGCA'G
Cuts at: 830 4122 4731 6940 7294 7300 8047 10128 10451
Size: 3292 609 2209 354 6 747 2081 323
Cuts at: 10451 830
Size: 3478
Fragments arranged by size:
3478 3292 2209 2081 747 609 354 323
6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13099
PvuII CAG'CTG
Cuts at: 2450 3463 3717 5000 5363 6863 7295 7304 7451
Size: 1013 254 1283 363 1500 432 9 147
Cuts at: 7451 9286 2450
Size: 1835 6263
Fragments arranged by size:
6263 1835 1500 1283 1013 432 363 254
147 9
SacI G_AGCT'C
Cuts at: 2458 2458
Size: 13099
SalI G'TCGA_C
Cuts at: 1936 4668 10441 1936
Size: 2732 5773 4594
Fragments arranged by size:
5773 4594 2732
SapI GCTCTTCn'nnn_
Cuts at: 3942 6096 3942
Size: 2154 10945
SbfI CC_TGCA'GG
Cuts at: 4122 10451 4122
Size: 6329 6770
ScaI AGT'ACT
Cuts at: 6508 11347 13059 6508
Size: 4839 1712 6548
Fragments arranged by size:
6548 4839 1712
SfcI C'TryA_G
Cuts at: 826 1045 3309 3315 3718 4118 4326 4614 4718
Size: 219 2264 6 403 400 208 288 104
Cuts at: 4718 4727 5226 6002 6936 7290 7296 8043 10124
Size: 9 499 776 934 354 6 747 2081
Cuts at: 10124 10447 11582 12263 12454 826
Size: 323 1135 681 191 1471
Fragments arranged by size:
2264 2081 1471 1135 934 776 747 681
499 403 400 354 323 288 219 208
191 104 9 6 6
SfoI GGC'GCC
Cuts at: 8573 8573
Size: 13099
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13099
SmaI CCC'GGG
Cuts at: 1872 10426 1872
Size: 8554 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 2457 2478 2886 3610 4688
Size: 197 926 246 268 21 408 724 1078
Cuts at: 4688 5440 7015 11203 12104 12366 820
Size: 752 1575 4188 901 262 1553
Fragments arranged by size:
4188 1575 1553 1078 926 901 752 724
408 268 262 246 197 21
SnaBI TAC'GTA
Cuts at: 493 10890 493
Size: 10397 2702
SpeI A'CTAG_T
Cuts at: 152 6217 152
Size: 6065 7034
SphI G_CATG'C
Cuts at: 1132 4778 10457 1132
Size: 3646 5679 3774
Fragments arranged by size:
5679 3774 3646
SspI AAT'ATT
Cuts at: 5 52 4242 4768 11023 5
Size: 47 4190 526 6255 2081
Fragments arranged by size:
6255 4190 2081 526 47
StuI AGG'CCT
Cuts at: 1517 2314 4664 9553 1517
Size: 797 2350 4889 5063
Fragments arranged by size:
5063 4889 2350 797
StyI C'CwwG_G
Cuts at: 513 1065 2336 4378 5238 5328 8098 8151 9339
Size: 552 1271 2042 860 90 2770 53 1188
Cuts at: 9339 9442 9548 10538 513
Size: 103 106 990 3074
Fragments arranged by size:
3074 2770 2042 1271 1188 990 860 552
106 103 90 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 2799 3331 4532 7181 8091 8639
Size: 1255 822 397 532 1201 2649 910 548
Cuts at: 8639 8984 11126 11143 11296 11481 11926 325
Size: 345 2142 17 153 185 445 1498
Fragments arranged by size:
2649 2142 1498 1255 1201 910 822 548
532 445 397 345 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2224 5842 6176
Size: 274 80 33 51 1443 247 3618 334
Cuts at: 6176 6506 7187 7202 7280 8824 9217 11345 13057
Size: 330 681 15 78 1544 393 2128 1712
Cuts at: 13057 96
Size: 138
Fragments arranged by size:
3618 2128 1712 1544 1443 681 393 334
330 274 247 138 80 78 51 33
15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3175 3207 4530 4562 5603 5635 6413 6445 9037
Size: 32 1323 32 1041 32 778 32 2592
Cuts at: 9037 9069 13059 13091 3175
Size: 32 3990 32 3183
Fragments arranged by size:
3990 3183 2592 1323 1041 778 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 9615 1316
Size: 8299 4800
XbaI T'CTAG_A
Cuts at: 8938 10435 8938
Size: 1497 11602
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 4465 4480 4751 2020
Size: 2445 15 271 10368
Fragments arranged by size:
10368 2445 271 15
XhoI C'TCGA_G
Cuts at: 1943 2457 2478 2886 1943
Size: 514 21 408 12156
Fragments arranged by size:
12156 514 408 21
XmaI C'CCGG_G
Cuts at: 1870 10424 1870
Size: 8554 4545
XmnI GAAnn'nnTTC
Cuts at: 9584 9852 11228 9584
Size: 268 1376 11455
Fragments arranged by size:
11455 1376 268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2010 2307 10907 276
Size: 53 83 186 936 476 297 8600 2468
Fragments arranged by size:
8600 2468 936 476 297 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BsiWI DraIII EcoRV
FseI FspAI NruI PacI PspOMI RsrII SacII SanDI
SexAI SfiI SrfI SwaI