Restriction map for
pFN21AA0892
(Circular) (Six-base) MAPSORT of: pFN21AA0892.seq Check: 8469 from: 1 to: 6544
pFN21AA0892 6544 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2217 2728 2217
Size: 511 6033
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4354 278
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2880 3887 1937
Size: 943 1007 4594
Fragments arranged by size:
4594 1007 943
Acc65I G'GTAC_C
Cuts at: 2079 3865 2079
Size: 1786 4758
AclI AA'CG_TT
Cuts at: 1563 4671 5044 1563
Size: 3108 373 3063
Fragments arranged by size:
3108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2783 3482 4607 5991 6259 1632
Size: 1151 699 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1151 1125 699 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6347
AflIII A'CryG_T
Cuts at: 1203 2865 3606 5443 6420 1203
Size: 1662 741 1837 977 1327
Fragments arranged by size:
1837 1662 1327 977 741
AgeI A'CCGG_T
Cuts at: 5375 5375
Size: 6544
AhdI GACnn_n'nnGTC
Cuts at: 2488 3780 5273 2488
Size: 1292 1493 3759
Fragments arranged by size:
3759 1493 1292
AleI CACnn'nnGTG
Cuts at: 1166 2868 1166
Size: 1702 4842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2597 1184
Size: 1413 5131
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 6512
AlwNI CAG_nnn'CTG
Cuts at: 3244 3718 4287 5859 3244
Size: 474 569 1572 3929
Fragments arranged by size:
3929 1572 569 474
ApaLI G'TGCA_C
Cuts at: 2147 2615 4601 5757 2147
Size: 468 1986 1156 2934
Fragments arranged by size:
2934 1986 1156 468
ApoI r'AATT_y
Cuts at: 1493 2321 2365 3853 4079 4231 6340 1493
Size: 828 44 1488 226 152 2109 1697
Fragments arranged by size:
2109 1697 1488 828 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 5098 160
Size: 872 4066 1606
Fragments arranged by size:
4066 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 3869 1870
Size: 73 586 1340 4545
Fragments arranged by size:
4545 1340 586 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3605 3638 3605
Size: 33 6511
BamHI G'GATC_C
Cuts at: 3874 4241 3874
Size: 367 6177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 3571 3865 5320 618
Size: 325 217 627 292 1492 294 1455 1842
Fragments arranged by size:
1842 1492 1455 627 325 294 292 217
BanII G_rGCy'C
Cuts at: 1391 2222 3405 3863 1391
Size: 831 1183 458 4072
Fragments arranged by size:
4072 1183 831 458
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2686 928
Size: 971 787 4786
Fragments arranged by size:
4786 971 787
BbvCI CC'TCA_GC
Cuts at: 2570 3477 2570
Size: 907 5637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4735 4769 1903
Size: 34 2798 34 3678
Fragments arranged by size:
3678 2798 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4436 5646 4436
Size: 1210 5334
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6544
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2036 2066
Size: 107 122 71 631 605 337 27 30
Cuts at: 2066 5155 136
Size: 3089 1525
Fragments arranged by size:
3089 1525 631 605 337 122 107 71
30 27
BglII A'GATC_T
Cuts at: 2409 3081 6539 2409
Size: 672 3458 2414
Fragments arranged by size:
3458 2414 672
BlpI GC'TnA_GC
Cuts at: 2103 3961 2103
Size: 1858 4686
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2619 4605 5761 1790
Size: 361 468 1986 1156 2573
Fragments arranged by size:
2573 1986 1156 468 361
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3199 4275 5233
Size: 416 53 83 399 24 1782 1076 958
Cuts at: 5233 442
Size: 1753
Fragments arranged by size:
1782 1753 1076 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2693 2942 2984 3044 3347 3707 5204
Size: 1052 402 249 42 60 303 360 1497
Cuts at: 5204 1239
Size: 2579
Fragments arranged by size:
2579 1497 1052 402 360 303 249 60
42
Bpu10I CC'TnA_GC
Cuts at: 2013 2570 3477 2013
Size: 557 907 5080
Fragments arranged by size:
5080 907 557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2957 4669 5534 5832 2495
Size: 462 1712 865 298 3207
Fragments arranged by size:
3207 1712 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3636 5207 882
Size: 617 2137 1571 2219
Fragments arranged by size:
2219 2137 1571 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 4335 493
Size: 711 2567 564 2702
Fragments arranged by size:
2702 2567 711 564
BsaBI GATnn'nnATC
Cuts at: 2408 6538 2408
Size: 4130 2414
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4351 4733 5368 275
Size: 53 83 186 936 2818 382 635 1451
Fragments arranged by size:
2818 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4977 5375 5649 5796 6207 4977
Size: 398 274 147 411 5314
Fragments arranged by size:
5314 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2264 2294 2852 2882 1178
Size: 30 165 30 861 30 558 30 4840
Fragments arranged by size:
4840 861 558 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2558 1186
Size: 1372 5172
BseYI C'CCAG_C
Cuts at: 2215 2822 3157 3538 5747 6243 2215
Size: 607 335 381 2209 496 2516
Fragments arranged by size:
2516 2209 607 496 381 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2450 2786 3131 3815 1163
Size: 573 714 336 345 684 3892
Fragments arranged by size:
3892 714 684 573 345 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 3994 4755 5783 1540
Size: 275 186 69 1924 761 1028 2301
Fragments arranged by size:
2301 1924 1028 761 275 186 69
BsiHKAI G_wGCw'C
Cuts at: 2109 2151 2619 2709 2865 3030 4605 4690 5761
Size: 42 468 90 156 165 1575 85 1071
Cuts at: 5761 2109
Size: 2892
Fragments arranged by size:
2892 1575 1071 468 165 156 90 85
42
BsmI GAATG_Cn'
Cuts at: 4055 4148 4055
Size: 93 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2151 2222 2619 2709 2865 3030
Size: 399 319 42 71 397 90 156 165
Cuts at: 3030 3405 3863 4605 4690 5761 1391
Size: 375 458 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 458 399 397 375 319
165 156 90 85 71 42
BspEI T'CCGG_A
Cuts at: 6207 6207
Size: 6544
BspHI T'CATG_A
Cuts at: 4433 6163 4433
Size: 1730 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2728 3899 844
Size: 1373 511 1171 3489
Fragments arranged by size:
3489 1373 1171 511
BsrBI CCG'CTC
Cuts at: 1394 1823 4431 1394
Size: 429 2608 3507
Fragments arranged by size:
3507 2608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3152 5039 5213 65
Size: 1183 1904 1887 174 1396
Fragments arranged by size:
1904 1887 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2912 3489 5188 5375 1700
Size: 110 1102 577 1699 187 2869
Fragments arranged by size:
2869 1699 1102 577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6544
BssHII G'CGCG_C
Cuts at: 1925 2258 1925
Size: 333 6211
BssSI C'ACGA_G
Cuts at: 2074 3509 4604 5616 2074
Size: 1435 1095 1012 3002
Fragments arranged by size:
3002 1435 1095 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2155 2614 2623 2905 3154 3718 794
Size: 1361 459 9 282 249 564 3620
Fragments arranged by size:
3620 1361 564 459 282 249 9
BstBI TT'CG_AA
Cuts at: 2310 5348 2310
Size: 3038 3506
BstEII G'GTnAC_C
Cuts at: 1184 2713 1184
Size: 1529 5015
BstXI CCAn_nnnn'nTGG
Cuts at: 3841 3841
Size: 6544
BstYI r'GATC_y
Cuts at: 1972 2409 2540 2814 3081 3597 3874 4241 4626
Size: 437 131 274 267 516 277 367 385
Cuts at: 4626 4643 6095 6181 6193 6539 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 516 437 385 367 346 277
274 267 131 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3066 3213 3669 513
Size: 552 939 1062 147 456 3388
Fragments arranged by size:
3388 1062 939 552 456 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5805
Fragments arranged by size:
5805 683 56
BtsI GCAGTG_nn'
Cuts at: 2149 2434 2783 2798 3367 3632 4060 4854 4874
Size: 285 349 15 569 265 428 794 20
Cuts at: 4874 2149
Size: 3819
Fragments arranged by size:
3819 794 569 428 349 285 265 20
15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6509
DraI TTT'AAA
Cuts at: 3848 3848
Size: 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5551 809
Size: 507 4235 1802
Fragments arranged by size:
4235 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 3831 3991
Size: 54 1425 50 275 18 237 1764 160
Cuts at: 3991 4880 6509 8
Size: 889 1629 43
Fragments arranged by size:
1764 1629 1425 889 275 237 160 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2067 3991 1537
Size: 275 255 1924 4090
Fragments arranged by size:
4090 1924 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 2453 2738 2846 3128 3410 4474 1353
Size: 1100 285 108 282 282 1064 3423
Fragments arranged by size:
3423 1100 1064 285 282 282 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2126 5131 5505 5651 387
Size: 1064 138 537 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 537 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2693 2783 2942 2984 3044 3347
Size: 393 659 402 90 159 42 60 303
Cuts at: 3347 3482 3707 4607 5204 5991 6259 1239
Size: 135 225 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 659 597 402 393 303
268 225 159 135 90 60 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2273 2573 2924 2273
Size: 300 351 5893
Fragments arranged by size:
5893 351 300
EcoO109I rG'GnC_Cy
Cuts at: 2482 2950 3481 2482
Size: 468 531 5545
Fragments arranged by size:
5545 531 468
EcoRI G'AATT_C
Cuts at: 2365 3853 4231 2365
Size: 1488 378 4678
Fragments arranged by size:
4678 1488 378
EcoRV GAT'ATC
Cuts at: 2289 2289
Size: 6544
FspI TGC'GCA
Cuts at: 2055 5050 2055
Size: 2995 3549
HaeII r_GCGC'y
Cuts at: 1678 2090 2561 3074 5691 1678
Size: 412 471 513 2617 2531
Fragments arranged by size:
2617 2531 513 471 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 3766 3798 5185
Size: 32 135 32 902 32 1260 32 1387
Cuts at: 5185 5217 5259 5291 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1387 1260 902 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3181 3888 4134 669
Size: 988 281 1243 707 246 3079
Fragments arranged by size:
3079 1243 988 707 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6544
HpaI GTT'AAC
Cuts at: 4134 4134
Size: 6544
KpnI G_GTAC'C
Cuts at: 2083 3869 2083
Size: 1786 4758
MfeI C'AATT_G
Cuts at: 4143 4143
Size: 6544
MluI A'CGCG_T
Cuts at: 6420 6420
Size: 6544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3153 5658 5842 1305
Size: 1848 2505 184 2007
Fragments arranged by size:
2505 2007 1848 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3833 10
Size: 54 1425 343 2001 2721
Fragments arranged by size:
2721 2001 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2808 2868 2870 3035 3569 4502 4861
Size: 648 1642 60 2 165 534 933 359
Cuts at: 4861 5020 518
Size: 159 2042
Fragments arranged by size:
2042 1642 933 648 534 359 165 159
60 2
MspA1I CmG'CkG
Cuts at: 1606 2027 2215 2389 2623 2770 2834 2962 3100
Size: 421 188 174 234 147 64 128 138
Cuts at: 3100 3145 3619 3718 3960 4637 5785 6030 1606
Size: 45 474 99 242 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 474 421 245 242 234
188 174 147 138 128 99 64 45
NaeI GCC'GGC
Cuts at: 2914 2914
Size: 6544
NcoI C'CATG_G
Cuts at: 513 1065 2004 3669 513
Size: 552 939 1665 3388
Fragments arranged by size:
3388 1665 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6544
NgoMIV G'CCGG_C
Cuts at: 2912 2912
Size: 6544
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6544
NotI GC'GGCC_GC
Cuts at: 3991 3991
Size: 6544
NspI r_CATG'y
Cuts at: 1132 1229 2869 3610 3902 5447 1132
Size: 97 1640 741 292 1545 2229
Fragments arranged by size:
2229 1640 1545 741 292 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6544
PciI A'CATG_T
Cuts at: 2865 3606 5443 2865
Size: 741 1837 3966
Fragments arranged by size:
3966 1837 741
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2725 1108
Size: 1617 4927
PfoI T'CCnGG_A
Cuts at: 3600 3723 3600
Size: 123 6421
PmeI GTTT'AAAC
Cuts at: 3848 3848
Size: 6544
PmlI CAC'GTG
Cuts at: 3771 3771
Size: 6544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3747 3779 4613 4645 6126 6158 3747
Size: 32 834 32 1481 32 4133
Fragments arranged by size:
4133 1481 834 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 3481 2482
Size: 999 5545
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6544
PsiI TTA'TAA
Cuts at: 4114 4114
Size: 6544
PstI C_TGCA'G
Cuts at: 830 2160 2214 2610 2775 2990 3078 3746 3896
Size: 1330 54 396 165 215 88 668 150
Cuts at: 3896 830
Size: 3478
Fragments arranged by size:
3478 1330 668 396 215 165 150 88
54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6544
PvuII CAG'CTG
Cuts at: 2215 2770 2962 3100 3145 3619 3718 2215
Size: 555 192 138 45 474 99 5041
Fragments arranged by size:
5041 555 474 192 138 99 45
SalI G'TCGA_C
Cuts at: 1936 3886 1936
Size: 1950 4594
SapI GCTCTTCn'nnn_
Cuts at: 2453 2738 3128 3410 2453
Size: 285 390 282 5587
Fragments arranged by size:
5587 390 285 282
SbfI CC_TGCA'GG
Cuts at: 2160 3896 2160
Size: 1736 4808
ScaI AGT'ACT
Cuts at: 4792 6504 4792
Size: 1712 4832
SexAI A'CCwGG_T
Cuts at: 2484 2883 2484
Size: 399 6145
SfcI C'TryA_G
Cuts at: 826 1045 2156 2210 2606 2771 2986 3074 3316
Size: 219 1111 54 396 165 215 88 242
Cuts at: 3316 3742 3892 5027 5708 5899 826
Size: 426 150 1135 681 191 1471
Fragments arranged by size:
1471 1135 1111 681 426 396 242 219
215 191 165 150 88 54
SfiI GGCCn_nnn'nGGCC
Cuts at: 2066 2066
Size: 6544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6544
SmaI CCC'GGG
Cuts at: 1872 2531 3871 1872
Size: 659 1340 4545
Fragments arranged by size:
4545 1340 659
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2474 2972 4648 5549 5811 820
Size: 197 926 531 498 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 531 498 262 197
SnaBI TAC'GTA
Cuts at: 493 4335 493
Size: 3842 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6544
SphI G_CATG'C
Cuts at: 1132 3902 1132
Size: 2770 3774
SspI AAT'ATT
Cuts at: 5 52 4468 5
Size: 47 4416 2081
Fragments arranged by size:
4416 2081 47
StuI AGG'CCT
Cuts at: 1517 3740 1517
Size: 2223 4321
StyI C'CwwG_G
Cuts at: 513 1065 2004 2856 3669 3983 513
Size: 552 939 852 813 314 3074
Fragments arranged by size:
3074 939 852 813 552 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2498 2732 4571 4588 4741 4926 5371
Size: 1255 918 234 1839 17 153 185 445
Cuts at: 5371 325
Size: 1498
Fragments arranged by size:
1839 1498 1255 918 445 234 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 2937 3165
Size: 274 80 33 51 1443 375 585 228
Cuts at: 3165 4790 6502 96
Size: 1625 1712 138
Fragments arranged by size:
1712 1625 1443 585 375 274 228 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2264 2283 2296 2315 2852 2884 6504 6536 2264
Size: 19 13 19 537 32 3620 32 2272
Fragments arranged by size:
3620 2272 537 32 32 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6544
XbaI T'CTAG_A
Cuts at: 3880 3880
Size: 6544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3809 3826 3809
Size: 17 6527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6544
XmaI C'CCGG_G
Cuts at: 1870 2529 3869 1870
Size: 659 1340 4545
Fragments arranged by size:
4545 1340 659
XmnI GAAnn'nnTTC
Cuts at: 4673 4673
Size: 6544
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4352 276
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI
KasI NarI NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfoI SrfI SwaI