Restriction map for
pFN21AA0882
(Circular) (Six-base) MAPSORT of: pFN21AA0882.seq Check: 1037 from: 1 to: 8503
pFN21AA0882 8503 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6313 278
Size: 53 83 186 936 4777 2468
Fragments arranged by size:
4777 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2226 2616 4308 5846 1937
Size: 289 390 1692 1538 4594
Fragments arranged by size:
4594 1692 1538 390 289
Acc65I G'GTAC_C
Cuts at: 2147 5824 2147
Size: 3677 4826
AclI AA'CG_TT
Cuts at: 1563 6630 7003 1563
Size: 5067 373 3063
Fragments arranged by size:
5067 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3810 6566 7950 8218 1632
Size: 2178 2756 1384 268 1917
Fragments arranged by size:
2756 2178 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8306
AflIII A'CryG_T
Cuts at: 1203 4900 5118 7402 8379 1203
Size: 3697 218 2284 977 1327
Fragments arranged by size:
3697 2284 1327 977 218
AgeI A'CCGG_T
Cuts at: 7334 7334
Size: 8503
AhdI GACnn_n'nnGTC
Cuts at: 7232 7232
Size: 8503
AleI CACnn'nnGTG
Cuts at: 1166 2345 3827 1166
Size: 1179 1482 5842
Fragments arranged by size:
5842 1482 1179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8503
AlwNI CAG_nnn'CTG
Cuts at: 3391 4396 4414 4847 5149 5653 6246 7818 3391
Size: 1005 18 433 302 504 593 1572 4076
Fragments arranged by size:
4076 1572 1005 593 504 433 302 18
ApaLI G'TGCA_C
Cuts at: 2892 3927 6560 7716 2892
Size: 1035 2633 1156 3679
Fragments arranged by size:
3679 2633 1156 1035
ApoI r'AATT_y
Cuts at: 1493 2444 4343 4990 5812 6038 6190 8299 1493
Size: 951 1899 647 822 226 152 2109 1697
Fragments arranged by size:
2109 1899 1697 951 822 647 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7057 160
Size: 872 6025 1606
Fragments arranged by size:
6025 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8503
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 3419 5424 5828 1870
Size: 73 235 1241 2005 404 4545
Fragments arranged by size:
4545 2005 1241 404 235 73
BamHI G'GATC_C
Cuts at: 5833 6200 5833
Size: 367 8136
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 3593 5824 7279 618
Size: 325 217 627 360 1446 2231 1455 1842
Fragments arranged by size:
2231 1842 1455 1446 627 360 325 217
BanII G_rGCy'C
Cuts at: 1391 3326 3576 5340 5822 1391
Size: 1935 250 1764 482 4072
Fragments arranged by size:
4072 1935 1764 482 250
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3639 5136 928
Size: 971 1740 1497 4295
Fragments arranged by size:
4295 1740 1497 971
BbvCI CC'TCA_GC
Cuts at: 2546 2546
Size: 8503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4588 4622 5194 5228 6694 6728 1903
Size: 34 2651 34 572 34 1466 34 3678
Fragments arranged by size:
3678 2651 1466 572 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4414 6395 7605 4414
Size: 1981 1210 5312
Fragments arranged by size:
5312 1981 1210
BclI T'GATC_A
Cuts at: 1548 2664 4828 1548
Size: 1116 2164 5223
Fragments arranged by size:
5223 2164 1116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2103 5271 5354
Size: 107 122 71 631 605 431 3168 83
Cuts at: 5354 7114 136
Size: 1760 1525
Fragments arranged by size:
3168 1760 1525 631 605 431 122 107
83 71
BglII A'GATC_T
Cuts at: 8498 8498
Size: 8503
BlpI GC'TnA_GC
Cuts at: 4277 5920 4277
Size: 1643 6860
Bme1580I G_kGCm'C
Cuts at: 1790 2896 3931 5591 6564 7720 1790
Size: 1106 1035 1660 973 1156 2573
Fragments arranged by size:
2573 1660 1156 1106 1035 973
BmgBI CAC'GTC
Cuts at: 1318 4807 1318
Size: 3489 5014
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 3099 4543
Size: 416 53 83 399 24 867 815 1444
Cuts at: 4543 5638 6234 7192 442
Size: 1095 596 958 1753
Fragments arranged by size:
1753 1444 1095 958 867 815 596 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3962 1056
Size: 2906 5597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 4756 4840 5392 5434 3292
Size: 1464 84 552 42 6361
Fragments arranged by size:
6361 1464 552 84 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2155 2196 3076 3286 4436 5013 5207 5429
Size: 916 41 880 210 1150 577 194 222
Cuts at: 5429 7163 1239
Size: 1734 2579
Fragments arranged by size:
2579 1734 1150 916 880 577 222 210
194 41
Bpu10I CC'TnA_GC
Cuts at: 2546 4815 2546
Size: 2269 6234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 2651 6628 7493 7791 2306
Size: 345 3977 865 298 3018
Fragments arranged by size:
3977 3018 865 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2717 3420 7166 882
Size: 617 1218 703 3746 2219
Fragments arranged by size:
3746 2219 1218 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 5320 6294 493
Size: 711 4116 974 2702
Fragments arranged by size:
4116 2702 974 711
BsaBI GATnn'nnATC
Cuts at: 8497 8497
Size: 8503
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6310 6692 7327 275
Size: 53 83 186 936 4777 382 635 1451
Fragments arranged by size:
4777 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2607 5742 6936 7334 7608 7755 8166 2607
Size: 3135 1194 398 274 147 411 2944
Fragments arranged by size:
3135 2944 1194 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 3524 3554 4234
Size: 30 165 30 609 30 1482 30 680
Cuts at: 4234 4253 4264 4283 5468 5498 1178
Size: 19 11 19 1185 30 4183
Fragments arranged by size:
4183 1482 1185 680 609 165 30 30
30 30 30 19 19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 3041 3289 3437 3692 3866 3968 5407
Size: 850 1005 248 148 255 174 102 1439
Cuts at: 5407 5434 5540 1186
Size: 27 106 4149
Fragments arranged by size:
4149 1439 1005 850 255 248 174 148
106 102 27
BseYI C'CCAG_C
Cuts at: 3284 3718 4571 5220 7706 8202 3284
Size: 434 853 649 2486 496 3585
Fragments arranged by size:
3585 2486 853 649 496 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2926 3200 3732 4095 4956 5522 1163
Size: 573 1190 274 532 363 861 566 4144
Fragments arranged by size:
4144 1190 861 573 566 532 363 274
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5953 6714 7742 1540
Size: 275 186 3952 761 1028 2301
Fragments arranged by size:
3952 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2678 2896 3102 3326 3576 3931 5289 5612 6564
Size: 218 206 224 250 355 1358 323 952
Cuts at: 6564 6649 7720 2678
Size: 85 1071 3461
Fragments arranged by size:
3461 1358 1071 952 355 323 250 224
218 206 85
BsmI GAATG_Cn'
Cuts at: 2644 5059 6014 6107 2644
Size: 2415 955 93 5040
Fragments arranged by size:
5040 2415 955 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3301 3301
Size: 8503
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2678 2896 3102 3326 3576 3931 5289
Size: 399 888 218 206 224 250 355 1358
Cuts at: 5289 5340 5591 5612 5822 6564 6649 7720 1391
Size: 51 251 21 210 742 85 1071 2174
Fragments arranged by size:
2174 1358 1071 888 742 399 355 251
250 224 218 210 206 85 51 21
BspEI T'CCGG_A
Cuts at: 5742 8166 5742
Size: 2424 6079
BspHI T'CATG_A
Cuts at: 6392 8122 6392
Size: 1730 6773
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5858 844
Size: 5014 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3055 3702 5244 5580 6390 1394
Size: 429 1232 647 1542 336 810 3507
Fragments arranged by size:
3507 1542 1232 810 647 429 336
BsrDI GCAATG_nn'
Cuts at: 65 1248 2370 3741 6998 7172 65
Size: 1183 1122 1371 3257 174 1396
Fragments arranged by size:
3257 1396 1371 1183 1122 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 4560 5344 5513 7147 7334 1700
Size: 110 292 2458 784 169 1634 187 2869
Fragments arranged by size:
2869 2458 1634 784 292 187 169 110
BsrGI T'GTAC_A
Cuts at: 96 4794 4895 96
Size: 4698 101 3704
Fragments arranged by size:
4698 3704 101
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8503
BssSI C'ACGA_G
Cuts at: 3319 3963 5522 6563 7575 3319
Size: 644 1559 1041 1012 4247
Fragments arranged by size:
4247 1559 1041 1012 644
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4810 794
Size: 4016 4487
BstBI TT'CG_AA
Cuts at: 3781 7307 3781
Size: 3526 4977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8503
BstXI CCAn_nnnn'nTGG
Cuts at: 3922 3922
Size: 8503
BstYI r'GATC_y
Cuts at: 1972 3186 3501 5833 6200 6585 6602 8054 8140
Size: 1214 315 2332 367 385 17 1452 86
Cuts at: 8140 8152 8498 1972
Size: 12 346 1977
Fragments arranged by size:
2332 1977 1452 1214 385 367 346 315
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4302 4302
Size: 8503
BtgI C'CryG_G
Cuts at: 513 1065 3651 3672 4762 5160 513
Size: 552 2586 21 1090 398 3856
Fragments arranged by size:
3856 2586 1090 552 398 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 2221 4296 487
Size: 683 56 895 100 2075 4694
Fragments arranged by size:
4694 2075 895 683 100 56
BtsI GCAGTG_nn'
Cuts at: 4244 5175 5257 5539 5597 6019 6813 6833 4244
Size: 931 82 282 58 422 794 20 5914
Fragments arranged by size:
5914 931 794 422 282 82 58 20
ClaI AT'CG_AT
Cuts at: 1554 2800 1554
Size: 1246 7257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8468
DraI TTT'AAA
Cuts at: 2428 5807 2428
Size: 3379 5124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2222 3944 7510 809
Size: 507 906 1722 3566 1802
Fragments arranged by size:
3566 1802 1722 906 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2929 3441
Size: 54 1425 50 275 18 204 895 512
Cuts at: 3441 5346 5581 5715 5769 5790 5950 6839 8468
Size: 1905 235 134 54 21 160 889 1629
Cuts at: 8468 8
Size: 43
Fragments arranged by size:
1905 1629 1425 895 889 512 275 235
204 160 134 54 54 50 43 21
18
EagI C'GGCC_G
Cuts at: 1537 1812 5950 1537
Size: 275 4138 4090
Fragments arranged by size:
4138 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 4638 4976 5294 6433 1353
Size: 1116 2169 338 318 1139 3423
Fragments arranged by size:
3423 2169 1139 1116 338 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2139 7090 7464 7610 387
Size: 1064 138 550 4951 374 146 1280
Fragments arranged by size:
4951 1280 1064 550 374 146 138
EcoICRI GAG'CTC
Cuts at: 3324 3574 3324
Size: 250 8253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2155 2196 3076 3286 3810 4436 5013
Size: 393 523 41 880 210 524 626 577
Cuts at: 5013 5207 5429 6566 7163 7950 8218 1239
Size: 194 222 1137 597 787 268 1524
Fragments arranged by size:
1524 1137 880 787 626 597 577 524
523 393 268 222 210 194 41
EcoO109I rG'GnC_Cy
Cuts at: 2521 4768 5436 2521
Size: 2247 668 5588
Fragments arranged by size:
5588 2247 668
EcoRI G'AATT_C
Cuts at: 5812 6190 5812
Size: 378 8125
EcoRV GAT'ATC
Cuts at: 2335 2335
Size: 8503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 8503
FspI TGC'GCA
Cuts at: 7009 7009
Size: 8503
HaeII r_GCGC'y
Cuts at: 1678 2178 3346 7650 1678
Size: 500 1168 4304 2531
Fragments arranged by size:
4304 2531 1168 500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3388 3420 3957 3989 5468
Size: 32 135 32 1816 32 537 32 1479
Cuts at: 5468 5500 7144 7176 7218 7250 1373
Size: 32 1644 32 42 32 2626
Fragments arranged by size:
2626 1816 1644 1479 537 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2488 4090 5847 6093 669
Size: 988 281 550 1602 1757 246 3079
Fragments arranged by size:
3079 1757 1602 988 550 281 246
HindIII A'AGCT_T
Cuts at: 748 3858 748
Size: 3110 5393
HpaI GTT'AAC
Cuts at: 6093 6093
Size: 8503
KpnI G_GTAC'C
Cuts at: 2151 5828 2151
Size: 3677 4826
MfeI C'AATT_G
Cuts at: 3069 6102 3069
Size: 3033 5470
MluI A'CGCG_T
Cuts at: 8379 8379
Size: 8503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2516 4888 7617 7801 1305
Size: 840 371 2372 2729 184 2007
Fragments arranged by size:
2729 2372 2007 840 371 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 2931 3443 5717 10
Size: 54 1425 343 204 895 512 2274 2796
Fragments arranged by size:
2796 2274 1425 895 512 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2345 2759 3827 5744 6461 6820
Size: 648 843 336 414 1068 1917 717 359
Cuts at: 6820 6979 518
Size: 159 2042
Fragments arranged by size:
2042 1917 1068 843 717 648 414 359
336 159
MspA1I CmG'CkG
Cuts at: 1606 2086 3982 4527 5919 6596 7744 7989 1606
Size: 480 1896 545 1392 677 1148 245 2120
Fragments arranged by size:
2120 1896 1392 1148 677 545 480 245
NaeI GCC'GGC
Cuts at: 2104 5346 5515 2104
Size: 3242 169 5092
Fragments arranged by size:
5092 3242 169
NcoI C'CATG_G
Cuts at: 513 1065 3651 4762 513
Size: 552 2586 1111 4254
Fragments arranged by size:
4254 2586 1111 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8503
NgoMIV G'CCGG_C
Cuts at: 2102 5344 5513 2102
Size: 3242 169 5092
Fragments arranged by size:
5092 3242 169
NheI G'CTAG_C
Cuts at: 1052 3958 1052
Size: 2906 5597
NotI GC'GGCC_GC
Cuts at: 5950 5950
Size: 8503
NspI r_CATG'y
Cuts at: 1132 1229 3511 3565 3895 3997 4653 4761 4904
Size: 97 2282 54 330 102 656 108 143
Cuts at: 4904 5122 5861 7406 1132
Size: 218 739 1545 2229
Fragments arranged by size:
2282 2229 1545 739 656 330 218 143
108 102 97 54
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8503
PciI A'CATG_T
Cuts at: 4900 5118 7402 4900
Size: 218 2284 6001
Fragments arranged by size:
6001 2284 218
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4414 1108
Size: 3306 5197
PfoI T'CCnGG_A
Cuts at: 2252 3552 4728 2252
Size: 1300 1176 6027
Fragments arranged by size:
6027 1300 1176
PmeI GTTT'AAAC
Cuts at: 5807 5807
Size: 8503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6572 6604 8085 8117 6572
Size: 32 1481 32 6958
Fragments arranged by size:
6958 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 4768 2521
Size: 2247 6256
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8503
PsiI TTA'TAA
Cuts at: 6073 6073
Size: 8503
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5105 5137 5105
Size: 32 8471
PstI C_TGCA'G
Cuts at: 830 2085 5855 830
Size: 1255 3770 3478
Fragments arranged by size:
3770 3478 1255
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8503
PvuII CAG'CTG
Cuts at: 3982 4527 3982
Size: 545 7958
SacI G_AGCT'C
Cuts at: 3326 3576 3326
Size: 250 8253
SalI G'TCGA_C
Cuts at: 1936 5845 1936
Size: 3909 4594
SapI GCTCTTCn'nnn_
Cuts at: 4638 5294 4638
Size: 656 7847
SbfI CC_TGCA'GG
Cuts at: 5855 5855
Size: 8503
ScaI AGT'ACT
Cuts at: 4868 5690 6751 8463 4868
Size: 822 1061 1712 4908
Fragments arranged by size:
4908 1712 1061 822
SfcI C'TryA_G
Cuts at: 826 1045 2081 5851 6986 7667 7858 826
Size: 219 1036 3770 1135 681 191 1471
Fragments arranged by size:
3770 1471 1135 1036 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5354 5354
Size: 8503
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8503
SgrAI Cr'CCGG_yG
Cuts at: 1700 2102 1700
Size: 402 8101
SmaI CCC'GGG
Cuts at: 1872 2180 5426 5830 1872
Size: 308 3246 404 4545
Fragments arranged by size:
4545 3246 404 308
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2285 2630 6607 7508 7770 820
Size: 197 926 342 345 3977 901 262 1553
Fragments arranged by size:
3977 1553 926 901 345 342 262 197
SnaBI TAC'GTA
Cuts at: 493 6294 493
Size: 5801 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8503
SphI G_CATG'C
Cuts at: 1132 3565 3895 3997 4761 5861 1132
Size: 2433 330 102 764 1100 3774
Fragments arranged by size:
3774 2433 1100 764 330 102
SspI AAT'ATT
Cuts at: 5 52 3817 6427 5
Size: 47 3765 2610 2081
Fragments arranged by size:
3765 2610 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8503
StyI C'CwwG_G
Cuts at: 513 1065 3651 3940 4643 4762 5380 5472 5942
Size: 552 2586 289 703 119 618 92 470
Cuts at: 5942 513
Size: 3074
Fragments arranged by size:
3074 2586 703 618 552 470 289 119
92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1999 3732 3914 6530 6547 6700 6885
Size: 1255 419 1733 182 2616 17 153 185
Cuts at: 6885 7330 325
Size: 445 1498
Fragments arranged by size:
2616 1733 1498 1255 445 419 185 182
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3273 4794 4866
Size: 274 80 33 51 1443 1296 1521 72
Cuts at: 4866 4895 5688 6749 8461 96
Size: 29 793 1061 1712 138
Fragments arranged by size:
1712 1521 1443 1296 1061 793 274 138
80 72 51 33 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4101 4133 4234 4253 4266 4285 5713 5745 8463
Size: 32 101 19 13 19 1428 32 2718
Cuts at: 8463 8495 4101
Size: 32 4109
Fragments arranged by size:
4109 2718 1428 101 32 32 32 19
19 13
Tth111I GACn'n_nGTC
Cuts at: 1316 3971 5564 1316
Size: 2655 1593 4255
Fragments arranged by size:
4255 2655 1593
XbaI T'CTAG_A
Cuts at: 5839 5839
Size: 8503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2105 4201 4480 5182 2105
Size: 2096 279 702 5426
Fragments arranged by size:
5426 2096 702 279
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8503
XmaI C'CCGG_G
Cuts at: 1870 2178 5424 5828 1870
Size: 308 3246 404 4545
Fragments arranged by size:
4545 3246 404 308
XmnI GAAnn'nnTTC
Cuts at: 6632 6632
Size: 8503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6311 276
Size: 53 83 186 936 4777 2468
Fragments arranged by size:
4777 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I DraIII EcoNI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacII SanDI SexAI SfoI SrfI SwaI