Restriction map for
pFN21AA0872
(Circular) (Six-base) MAPSORT of: pFN21AA0872.seq Check: 2987 from: 1 to: 6346
pFN21AA0872 6346 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3491 3491
Size: 6346
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4156 278
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2851 3689 1937
Size: 914 838 4594
Fragments arranged by size:
4594 914 838
Acc65I G'GTAC_C
Cuts at: 3667 3667
Size: 6346
AclI AA'CG_TT
Cuts at: 1563 4473 4846 1563
Size: 2910 373 3063
Fragments arranged by size:
3063 2910 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4409 5793 6061 1632
Size: 2777 1384 268 1917
Fragments arranged by size:
2777 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6149
AflIII A'CryG_T
Cuts at: 1203 2261 2609 5245 6222 1203
Size: 1058 348 2636 977 1327
Fragments arranged by size:
2636 1327 1058 977 348
AgeI A'CCGG_T
Cuts at: 5177 5177
Size: 6346
AhdI GACnn_n'nnGTC
Cuts at: 5075 5075
Size: 6346
AleI CACnn'nnGTG
Cuts at: 1166 2977 1166
Size: 1811 4535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2995 1184
Size: 1811 4535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 2054
Size: 32 6314
AlwNI CAG_nnn'CTG
Cuts at: 2246 2816 2923 3346 4089 5661 2246
Size: 570 107 423 743 1572 2931
Fragments arranged by size:
2931 1572 743 570 423 107
ApaI G_GGCC'C
Cuts at: 2513 2513
Size: 6346
ApaLI G'TGCA_C
Cuts at: 2582 2940 4403 5559 2582
Size: 358 1463 1156 3369
Fragments arranged by size:
3369 1463 1156 358
ApoI r'AATT_y
Cuts at: 1493 3458 3655 3881 4033 6142 1493
Size: 1965 197 226 152 2109 1697
Fragments arranged by size:
2109 1965 1697 226 197 152
AseI AT'TA_AT
Cuts at: 160 1032 4900 160
Size: 872 3868 1606
Fragments arranged by size:
3868 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 3671 1870
Size: 73 96 1632 4545
Fragments arranged by size:
4545 1632 96 73
BamHI G'GATC_C
Cuts at: 2122 2153 3676 4043 2122
Size: 31 1523 367 4425
Fragments arranged by size:
4425 1523 367 31
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2741 2971 3065 3105 3137
Size: 325 217 627 954 230 94 40 32
Cuts at: 3137 3223 3667 5122 618
Size: 86 444 1455 1842
Fragments arranged by size:
1842 1455 954 627 444 325 230 217
94 86 40 32
BanII G_rGCy'C
Cuts at: 1391 2513 2731 3468 3665 1391
Size: 1122 218 737 197 4072
Fragments arranged by size:
4072 1122 737 218 197
BbeI G_GCGC'C
Cuts at: 3109 3227 3109
Size: 118 6228
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 928
Size: 971 237 5138
Fragments arranged by size:
5138 971 237
BbvCI CC'TCA_GC
Cuts at: 2478 3479 2478
Size: 1001 5345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4537 4571 1903
Size: 34 2600 34 3678
Fragments arranged by size:
3678 2600 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4238 5448 4238
Size: 1210 5136
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6346
BfrBI ATG'CAT
Cuts at: 3127 3127
Size: 6346
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2954 3023 4957
Size: 107 122 71 631 605 1282 69 1934
Cuts at: 4957 136
Size: 1525
Fragments arranged by size:
1934 1525 1282 631 605 122 107 71
69
BglII A'GATC_T
Cuts at: 6341 6341
Size: 6346
BlpI GC'TnA_GC
Cuts at: 3763 3763
Size: 6346
Bme1580I G_kGCm'C
Cuts at: 1790 2513 2586 2760 2944 2974 3151 4407 5563
Size: 723 73 174 184 30 177 1256 1156
Cuts at: 5563 1790
Size: 2573
Fragments arranged by size:
2573 1256 1156 723 184 177 174 73
30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6346
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2070 2191 2298
Size: 416 53 83 399 24 653 121 107
Cuts at: 2298 2941 3283 4077 5035 442
Size: 643 342 794 958 1753
Fragments arranged by size:
1753 958 794 653 643 416 399 342
121 107 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2579 1056
Size: 1523 4823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2296 2750 2296
Size: 454 5892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2251 2304 5006 1239
Size: 923 89 53 2702 2579
Fragments arranged by size:
2702 2579 923 89 53
Bpu10I CC'TnA_GC
Cuts at: 2478 3303 3479 2478
Size: 825 176 5345
Fragments arranged by size:
5345 825 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4471 5336 5634 4471
Size: 865 298 5183
Fragments arranged by size:
5183 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5009 882
Size: 617 3510 2219
Fragments arranged by size:
3510 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2612 3022 3418 4137 493
Size: 711 1408 410 396 719 2702
Fragments arranged by size:
2702 1408 719 711 410 396
BsaBI GATnn'nnATC
Cuts at: 6340 6340
Size: 6346
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 3224 4153 4535
Size: 53 83 186 936 1573 118 929 382
Cuts at: 4535 5170 275
Size: 635 1451
Fragments arranged by size:
1573 1451 936 929 635 382 186 118
83 53
BsaWI w'CCGG_w
Cuts at: 4779 5177 5451 5598 6009 4779
Size: 398 274 147 411 5116
Fragments arranged by size:
5116 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2384 2414 3374 3404 1178
Size: 30 165 30 981 30 960 30 4120
Fragments arranged by size:
4120 981 960 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 2327 2786 3344 3347 3563 1186
Size: 845 296 459 558 3 216 3969
Fragments arranged by size:
3969 845 558 459 296 216 3
BseYI C'CCAG_C
Cuts at: 2177 2556 2905 3534 5549 6045 2177
Size: 379 349 629 2015 496 2478
Fragments arranged by size:
2478 2015 629 496 379 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 3230 1163
Size: 573 726 768 4279
Fragments arranged by size:
4279 768 726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2851 3796 4557 5585 1540
Size: 275 186 850 945 761 1028 2301
Fragments arranged by size:
2301 1028 945 850 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2586 2603 2944 3196 3468 4407 4492 5563 2586
Size: 17 341 252 272 939 85 1071 3369
Fragments arranged by size:
3369 1071 939 341 272 252 85 17
BsmI GAATG_Cn'
Cuts at: 3857 3950 3857
Size: 93 6253
BsmBI CGTCTCn'nnnn_
Cuts at: 2772 3383 2772
Size: 611 5735
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2513 2586 2603 2731 2760 2944 2974
Size: 399 723 73 17 128 29 184 30
Cuts at: 2974 3151 3196 3468 3665 4407 4492 5563 1391
Size: 177 45 272 197 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 399 272 197 184
177 128 85 73 45 30 29 17
BspEI T'CCGG_A
Cuts at: 6009 6009
Size: 6346
BspHI T'CATG_A
Cuts at: 4235 5965 4235
Size: 1730 4616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3482 3491 3701 844
Size: 2078 560 9 210 3489
Fragments arranged by size:
3489 2078 560 210 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2875 3337 4233 1394
Size: 429 1052 462 896 3507
Fragments arranged by size:
3507 1052 896 462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4841 5015 65
Size: 1183 3593 174 1396
Fragments arranged by size:
3593 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2895 3102 3555 4990 5177 1700
Size: 110 1085 207 453 1435 187 2869
Fragments arranged by size:
2869 1435 1085 453 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6346
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6346
BssSI C'ACGA_G
Cuts at: 2522 4406 5418 2522
Size: 1884 1012 3450
Fragments arranged by size:
3450 1884 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2789 2923 794
Size: 1995 134 4217
Fragments arranged by size:
4217 1995 134
BstBI TT'CG_AA
Cuts at: 5150 5150
Size: 6346
BstEII G'GTnAC_C
Cuts at: 1184 3448 3490 1184
Size: 2264 42 4040
Fragments arranged by size:
4040 2264 42
BstYI r'GATC_y
Cuts at: 1972 2122 2153 3676 4043 4428 4445 5897 5983
Size: 150 31 1523 367 385 17 1452 86
Cuts at: 5983 5995 6341 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1523 1452 385 367 346 150 86
31 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2409 3090 513
Size: 552 939 405 681 3769
Fragments arranged by size:
3769 939 681 552 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5607
Fragments arranged by size:
5607 683 56
BtsI GCAGTG_nn'
Cuts at: 2022 3862 4656 4676 2022
Size: 1840 794 20 3692
Fragments arranged by size:
3692 1840 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6311
DraI TTT'AAA
Cuts at: 3650 3650
Size: 6346
DraIII CAC_nnn'GTG
Cuts at: 2789 2789
Size: 6346
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2545 5353 809
Size: 507 1229 2808 1802
Fragments arranged by size:
2808 1802 1229 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 2886 3093
Size: 54 1425 50 275 18 696 360 207
Cuts at: 3093 3793 4682 6311 8
Size: 700 889 1629 43
Fragments arranged by size:
1629 1425 889 700 696 360 275 207
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3793 1537
Size: 275 1981 4090
Fragments arranged by size:
4090 1981 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3566 4276 1353
Size: 2213 710 3423
Fragments arranged by size:
3423 2213 710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2648 4933 5307 5453 387
Size: 1064 138 1059 2285 374 146 1280
Fragments arranged by size:
2285 1280 1064 1059 374 146 138
EcoICRI GAG'CTC
Cuts at: 3466 3466
Size: 6346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2251 2304 4409 5006 5793 6061
Size: 393 530 89 53 2105 597 787 268
Cuts at: 6061 1239
Size: 1524
Fragments arranged by size:
2105 1524 787 597 530 393 268 89
53
EcoNI CCTnn'n_nnAGG
Cuts at: 2504 3155 2504
Size: 651 5695
EcoO109I rG'GnC_Cy
Cuts at: 2137 2206 2299 2509 2719 3313 2137
Size: 69 93 210 210 594 5170
Fragments arranged by size:
5170 594 210 210 93 69
EcoRI G'AATT_C
Cuts at: 3655 4033 3655
Size: 378 5968
FspI TGC'GCA
Cuts at: 4852 4852
Size: 6346
HaeII r_GCGC'y
Cuts at: 1678 2399 2816 2828 3109 3227 5493 1678
Size: 721 417 12 281 118 2266 2531
Fragments arranged by size:
2531 2266 721 417 281 118 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2384 2416 2445
Size: 32 135 32 623 32 157 32 29
Cuts at: 2445 2477 4987 5019 5061 5093 1373
Size: 32 2510 32 42 32 2626
Fragments arranged by size:
2626 2510 623 157 135 42 32 32
32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2852 3690 3936 669
Size: 988 281 914 838 246 3079
Fragments arranged by size:
3079 988 914 838 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6346
HpaI GTT'AAC
Cuts at: 3936 3936
Size: 6346
KasI G'GCGC_C
Cuts at: 3105 3223 3105
Size: 118 6228
KpnI G_GTAC'C
Cuts at: 3671 3671
Size: 6346
MfeI C'AATT_G
Cuts at: 3945 3945
Size: 6346
MluI A'CGCG_T
Cuts at: 6222 6222
Size: 6346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1993 2824 3330 5460 5644 1305
Size: 688 831 506 2130 184 2007
Fragments arranged by size:
2130 2007 831 688 506 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 10
Size: 54 1425 343 696 3828
Fragments arranged by size:
3828 1425 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2977 4304 4663 4822 518
Size: 648 1811 1327 359 159 2042
Fragments arranged by size:
2042 1811 1327 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2329 2560 2773 2909 3256 3343 3762 4439
Size: 723 231 213 136 347 87 419 677
Cuts at: 4439 5587 5832 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 723 677 419 347 245 231
213 136 87
NaeI GCC'GGC
Cuts at: 2897 3104 2897
Size: 207 6139
NarI GG'CG_CC
Cuts at: 3106 3224 3106
Size: 118 6228
NcoI C'CATG_G
Cuts at: 513 1065 2004 2409 3090 513
Size: 552 939 405 681 3769
Fragments arranged by size:
3769 939 681 552 405
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6346
NgoMIV G'CCGG_C
Cuts at: 2895 3102 2895
Size: 207 6139
NheI G'CTAG_C
Cuts at: 1052 2575 1052
Size: 1523 4823
NotI GC'GGCC_GC
Cuts at: 3793 3793
Size: 6346
NsiI A_TGCA'T
Cuts at: 3129 3129
Size: 6346
NspI r_CATG'y
Cuts at: 1132 1229 2265 2339 3131 3374 3704 5249 1132
Size: 97 1036 74 792 243 330 1545 2229
Fragments arranged by size:
2229 1545 1036 792 330 243 97 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6346
PciI A'CATG_T
Cuts at: 2261 5245 2261
Size: 2984 3362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2491 3091 1108
Size: 1383 600 4363
Fragments arranged by size:
4363 1383 600
PmeI GTTT'AAAC
Cuts at: 3650 3650
Size: 6346
PmlI CAC'GTG
Cuts at: 2612 3418 2612
Size: 806 5540
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 5928 5960 4415
Size: 32 1481 32 4801
Fragments arranged by size:
4801 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2719 2299
Size: 420 5926
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6346
PsiI TTA'TAA
Cuts at: 2858 3916 2858
Size: 1058 5288
PspOMI G'GGCC_C
Cuts at: 2509 2509
Size: 6346
PstI C_TGCA'G
Cuts at: 830 2242 2919 3698 830
Size: 1412 677 779 3478
Fragments arranged by size:
3478 1412 779 677
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6346
PvuII CAG'CTG
Cuts at: 2560 2909 2560
Size: 349 5997
SacI G_AGCT'C
Cuts at: 3468 3468
Size: 6346
SalI G'TCGA_C
Cuts at: 1936 2850 3688 1936
Size: 914 838 4594
Fragments arranged by size:
4594 914 838
SanDI GG'GwC_CC
Cuts at: 2299 2299
Size: 6346
SapI GCTCTTCn'nnn_
Cuts at: 3566 3566
Size: 6346
SbfI CC_TGCA'GG
Cuts at: 3698 3698
Size: 6346
ScaI AGT'ACT
Cuts at: 3584 4594 6306 3584
Size: 1010 1712 3624
Fragments arranged by size:
3624 1712 1010
SfcI C'TryA_G
Cuts at: 826 1045 2238 2915 3202 3319 3694 4829 5510
Size: 219 1193 677 287 117 375 1135 681
Cuts at: 5510 5701 826
Size: 191 1471
Fragments arranged by size:
1471 1193 1135 681 677 375 287 219
191 117
SfoI GGC'GCC
Cuts at: 3107 3225 3107
Size: 118 6228
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6346
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6346
SmaI CCC'GGG
Cuts at: 1872 2041 3673 1872
Size: 169 1632 4545
Fragments arranged by size:
4545 1632 169
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4450 5351 5613 820
Size: 197 926 2507 901 262 1553
Fragments arranged by size:
2507 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4137 493
Size: 3644 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6346
SphI G_CATG'C
Cuts at: 1132 3131 3374 3704 1132
Size: 1999 243 330 3774
Fragments arranged by size:
3774 1999 330 243
SspI AAT'ATT
Cuts at: 5 52 4270 5
Size: 47 4218 2081
Fragments arranged by size:
4218 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6346
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2409 3090 3133 3151 3495
Size: 552 939 153 252 681 43 18 344
Cuts at: 3495 3785 513
Size: 290 3074
Fragments arranged by size:
3074 939 681 552 344 290 252 153
43 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 2402 2709 2729 2923 2927 3083
Size: 1255 611 211 307 20 194 4 156
Cuts at: 3083 3436 3526 3592 4373 4390 4543 4728 5173
Size: 353 90 66 781 17 153 185 445
Cuts at: 5173 325
Size: 1498
Fragments arranged by size:
1498 1255 781 611 445 353 307 211
194 185 156 153 90 66 20 17
4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3582 4592 6304
Size: 274 80 33 51 1443 1605 1010 1712
Cuts at: 6304 96
Size: 138
Fragments arranged by size:
1712 1605 1443 1010 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 6306 6338 3374
Size: 32 2900 32 3382
Fragments arranged by size:
3382 2900 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6346
XbaI T'CTAG_A
Cuts at: 3682 3682
Size: 6346
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6346
XmaI C'CCGG_G
Cuts at: 1870 2039 3671 1870
Size: 169 1632 4545
Fragments arranged by size:
4545 1632 169
XmnI GAAnn'nnTTC
Cuts at: 4475 4475
Size: 6346
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4154 276
Size: 53 83 186 936 2620 2468
Fragments arranged by size:
2620 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI BstXI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NruI PacI PfoI PspXI
PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI