Restriction map for
pFN21AA0869
(Circular) (Six-base) MAPSORT of: pFN21AA0869.seq Check: 8543 from: 1 to: 8044
pFN21AA0869 8044 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3238 3238
Size: 8044
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5343 5854 278
Size: 53 83 186 936 3807 511 2468
Fragments arranged by size:
3807 2468 936 511 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2070 4334 5387 1937
Size: 133 2264 1053 4594
Fragments arranged by size:
4594 2264 1053 133
Acc65I G'GTAC_C
Cuts at: 4647 5365 4647
Size: 718 7326
AclI AA'CG_TT
Cuts at: 1563 6171 6544 1563
Size: 4608 373 3063
Fragments arranged by size:
4608 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 2546 2609 2978 3077 3363 3918 4022
Size: 680 234 63 369 99 286 555 104
Cuts at: 4022 5060 5171 6107 7491 7759 1632
Size: 1038 111 936 1384 268 1917
Fragments arranged by size:
1917 1384 1038 936 680 555 369 286
268 234 111 104 99 63
AflII C'TTAA_G
Cuts at: 820 1017 4234 820
Size: 197 3217 4630
Fragments arranged by size:
4630 3217 197
AflIII A'CryG_T
Cuts at: 1203 4818 6943 7920 1203
Size: 3615 2125 977 1327
Fragments arranged by size:
3615 2125 1327 977
AgeI A'CCGG_T
Cuts at: 6875 6875
Size: 8044
AhdI GACnn_n'nnGTC
Cuts at: 2764 2782 4444 4904 6773 2764
Size: 18 1662 460 1869 4035
Fragments arranged by size:
4035 1869 1662 460 18
AleI CACnn'nnGTG
Cuts at: 1166 4175 4709 5178 1166
Size: 3009 534 469 4032
Fragments arranged by size:
4032 3009 534 469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2415 1184
Size: 1231 6813
AlwNI CAG_nnn'CTG
Cuts at: 2365 2395 2587 2836 3301 3565 3887 3925 3941
Size: 30 192 249 465 264 322 38 16
Cuts at: 3941 4427 4516 5787 7359 2365
Size: 486 89 1271 1572 3050
Fragments arranged by size:
3050 1572 1271 486 465 322 264 249
192 89 38 30 16
ApaLI G'TGCA_C
Cuts at: 2328 5225 6101 7257 2328
Size: 2897 876 1156 3115
Fragments arranged by size:
3115 2897 1156 876
ApoI r'AATT_y
Cuts at: 1493 3987 5353 5579 5731 7840 1493
Size: 2494 1366 226 152 2109 1697
Fragments arranged by size:
2494 2109 1697 1366 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4037 6598 160
Size: 872 3005 2561 1606
Fragments arranged by size:
3005 2561 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8044
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 2511 2863 3156 3487 5369 1870
Size: 73 313 255 352 293 331 1882 4545
Fragments arranged by size:
4545 1882 352 331 313 293 255 73
BamHI G'GATC_C
Cuts at: 2251 3323 5374 5741 2251
Size: 1072 2051 367 4554
Fragments arranged by size:
4554 2051 1072 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3581 4289 4647 4736 5365
Size: 325 217 627 1794 708 358 89 629
Cuts at: 5365 6820 618
Size: 1455 1842
Fragments arranged by size:
1842 1794 1455 708 629 627 358 325
217 89
BanII G_rGCy'C
Cuts at: 1391 2426 2849 3347 4392 5049 5363 1391
Size: 1035 423 498 1045 657 314 4072
Fragments arranged by size:
4072 1045 1035 657 498 423 314
BbeI G_GCGC'C
Cuts at: 4293 4293
Size: 8044
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3714 4235 4527 928
Size: 971 1815 521 292 4445
Fragments arranged by size:
4445 1815 971 521 292
BbvCI CC'TCA_GC
Cuts at: 5135 5135
Size: 8044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6235 6269 1903
Size: 34 50 34 4214 34 3678
Fragments arranged by size:
4214 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4210 5936 7146 4210
Size: 1726 1210 5108
Fragments arranged by size:
5108 1726 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8044
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 4287 6655
Size: 107 122 71 631 605 646 1969 2368
Cuts at: 6655 136
Size: 1525
Fragments arranged by size:
2368 1969 1525 646 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2340 2713 5028 8039 2340
Size: 373 2315 3011 2345
Fragments arranged by size:
3011 2345 2315 373
BlpI GC'TnA_GC
Cuts at: 5461 5461
Size: 8044
Bme1580I G_kGCm'C
Cuts at: 1790 2332 4739 5229 6105 7261 1790
Size: 542 2407 490 876 1156 2573
Fragments arranged by size:
2573 2407 1156 876 542 490
BmgBI CAC'GTC
Cuts at: 1318 3333 1318
Size: 2015 6029
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3935 5775 6733
Size: 416 53 83 399 24 2518 1840 958
Cuts at: 6733 442
Size: 1753
Fragments arranged by size:
2518 1840 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8044
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3744 4353 4645 3744
Size: 609 292 7143
Fragments arranged by size:
7143 609 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2531 2822 3134 3251 3482 3797 3909
Size: 1148 144 291 312 117 231 315 112
Cuts at: 3909 4499 4549 4913 4997 5165 6704 1239
Size: 590 50 364 84 168 1539 2579
Fragments arranged by size:
2579 1539 1148 590 364 315 312 291
231 168 144 117 112 84 50
Bpu10I CC'TnA_GC
Cuts at: 2619 2646 5135 2619
Size: 27 2489 5528
Fragments arranged by size:
5528 2489 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2677 3268 4376 5033 6169 7034 7332 2677
Size: 591 1108 657 1136 865 298 3389
Fragments arranged by size:
3389 1136 1108 865 657 591 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2446 2772 4535 4667 4679 6707 882
Size: 617 947 326 1763 132 12 2028 2219
Fragments arranged by size:
2219 2028 1763 947 617 326 132 12
BsaAI yAC'GTr
Cuts at: 493 1204 4819 5835 493
Size: 711 3615 1016 2702
Fragments arranged by size:
3615 2702 1016 711
BsaBI GATnn'nnATC
Cuts at: 3032 5315 8038 3032
Size: 2283 2723 3038
Fragments arranged by size:
3038 2723 2283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4161 4290 5340 5851
Size: 53 83 186 936 2628 129 1050 511
Cuts at: 5851 6233 6868 275
Size: 382 635 1451
Fragments arranged by size:
2628 1451 1050 936 635 511 382 186
129 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4725 4908 6477 6875 7149 7296 7707 4143
Size: 582 183 1569 398 274 147 411 4480
Fragments arranged by size:
4480 1569 582 411 398 274 183 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4828 4858 1178
Size: 30 165 30 3425 30 4364
Fragments arranged by size:
4364 3425 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 3062 3361 3616 4553 4589 4592 4595
Size: 871 1005 299 255 937 36 3 3
Cuts at: 4595 4604 4607 4610 1186
Size: 9 3 3 4620
Fragments arranged by size:
4620 1005 937 871 299 255 36 9
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2037 2419 3267 3805 4651 5320 7247 7743 2037
Size: 382 848 538 846 669 1927 496 2338
Fragments arranged by size:
2338 1927 848 846 669 538 496 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3245 3938 1163
Size: 573 1509 693 5269
Fragments arranged by size:
5269 1509 693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2015 5494 6255 7283 1540
Size: 275 186 14 3479 761 1028 2301
Fragments arranged by size:
3479 2301 1028 761 275 186 14
BsiHKAI G_wGCw'C
Cuts at: 2332 2849 4392 4772 5049 5175 5229 6105 6190
Size: 517 1543 380 277 126 54 876 85
Cuts at: 6190 7261 2332
Size: 1071 3115
Fragments arranged by size:
3115 1543 1071 876 517 380 277 126
85 54
BsmI GAATG_Cn'
Cuts at: 5555 5648 5555
Size: 93 7951
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2426 2849 3347 4392 4739 4772
Size: 399 542 94 423 498 1045 347 33
Cuts at: 4772 5049 5175 5229 5363 6105 6190 7261 1391
Size: 277 126 54 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 1045 742 542 498 423 399
347 277 134 126 94 85 54 33
BspEI T'CCGG_A
Cuts at: 4143 4908 7707 4143
Size: 765 2799 4480
Fragments arranged by size:
4480 2799 765
BspHI T'CATG_A
Cuts at: 5933 7663 5933
Size: 1730 6314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3153 3238 4142 4995 5399 844
Size: 2309 85 904 853 404 3489
Fragments arranged by size:
3489 2309 904 853 404 85
BsrBI CCG'CTC
Cuts at: 1394 1823 2137 4782 5168 5931 1394
Size: 429 314 2645 386 763 3507
Fragments arranged by size:
3507 2645 763 429 386 314
BsrDI GCAATG_nn'
Cuts at: 65 1248 6539 6713 65
Size: 1183 5291 174 1396
Fragments arranged by size:
5291 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3021 3473 3771 4317 4824 6688 6875
Size: 110 1211 452 298 546 507 1864 187
Cuts at: 6875 1700
Size: 2869
Fragments arranged by size:
2869 1864 1211 546 507 452 298 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8044
BssHII G'CGCG_C
Cuts at: 1925 2048 1925
Size: 123 7921
BssSI C'ACGA_G
Cuts at: 2552 3509 6104 7116 2552
Size: 957 2595 1012 3480
Fragments arranged by size:
3480 2595 1012 957
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2557 3565 3977 4134 794
Size: 1763 1008 412 157 4704
Fragments arranged by size:
4704 1763 1008 412 157
BstBI TT'CG_AA
Cuts at: 4882 6848 4882
Size: 1966 6078
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8044
BstXI CCAn_nnnn'nTGG
Cuts at: 3836 3836
Size: 8044
BstYI r'GATC_y
Cuts at: 1972 2251 2340 2478 2713 2961 3323 5028 5374
Size: 279 89 138 235 248 362 1705 346
Cuts at: 5374 5741 6126 6143 7595 7681 7693 8039 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1705 1452 385 367 362 346 346
279 248 235 138 89 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3307 4420 4719 3307
Size: 1113 299 6632
Fragments arranged by size:
6632 1113 299
BtgI C'CryG_G
Cuts at: 513 1065 2114 5077 513
Size: 552 1049 2963 3480
Fragments arranged by size:
3480 2963 1049 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7305
Fragments arranged by size:
7305 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 3978 4476 5560 6354 6374 2499
Size: 1479 498 1084 794 20 4169
Fragments arranged by size:
4169 1479 1084 794 498 20
ClaI AT'CG_AT
Cuts at: 1554 2598 1554
Size: 1044 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2227 2262 2554 2589 5085 5120 529
Size: 35 1663 35 292 35 2496 35 3453
Fragments arranged by size:
3453 2496 1663 292 35 35 35 35
DraI TTT'AAA
Cuts at: 5348 5348
Size: 8044
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2066 7051 809
Size: 507 750 4985 1802
Fragments arranged by size:
4985 1802 750 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 2970 3522
Size: 54 1425 50 275 18 182 958 552
Cuts at: 3522 3550 5491 6380 8009 8
Size: 28 1941 889 1629 43
Fragments arranged by size:
1941 1629 1425 958 889 552 275 182
54 50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2012 5491 1537
Size: 275 200 3479 4090
Fragments arranged by size:
4090 3479 275 200
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2522 2925 4269 4478 4566 4599 5974
Size: 825 344 403 1344 209 88 33 1375
Cuts at: 5974 1353
Size: 3423
Fragments arranged by size:
3423 1375 1344 825 403 344 209 88
33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4322 5114 6631 7005 7151 387
Size: 1064 138 2733 792 1517 374 146 1280
Fragments arranged by size:
2733 1517 1280 1064 792 374 146 138
EcoICRI GAG'CTC
Cuts at: 2847 4390 5047 2847
Size: 1543 657 5844
Fragments arranged by size:
5844 1543 657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2387 2531 2546 2609 2822 2978
Size: 393 680 75 144 15 63 213 156
Cuts at: 2978 3077 3134 3251 3363 3482 3797 3909 3918
Size: 99 57 117 112 119 315 112 9
Cuts at: 3918 4022 4499 4549 4913 4997 5060 5165 5171
Size: 104 477 50 364 84 63 105 6
Cuts at: 5171 6107 6704 7491 7759 1239
Size: 936 597 787 268 1524
Fragments arranged by size:
1524 936 787 680 597 477 393 364
315 268 213 156 144 119 117 112
112 105 104 99 84 75 63 63
57 50 15 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 4307 4419 2030
Size: 2277 112 5655
Fragments arranged by size:
5655 2277 112
EcoO109I rG'GnC_Cy
Cuts at: 3142 4424 4802 3142
Size: 1282 378 6384
Fragments arranged by size:
6384 1282 378
EcoRI G'AATT_C
Cuts at: 3987 5353 5731 3987
Size: 1366 378 6300
Fragments arranged by size:
6300 1366 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 8044
FspI TGC'GCA
Cuts at: 6550 6550
Size: 8044
HaeII r_GCGC'y
Cuts at: 1678 2365 3179 3759 4293 7191 1678
Size: 687 814 580 534 2898 2531
Fragments arranged by size:
2898 2531 814 687 580 534
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2236 2268 2750 2768 2782
Size: 32 135 32 664 32 482 18 14
Cuts at: 2782 2800 2831 2863 4138 4170 4230 4262 4430
Size: 18 31 32 1275 32 60 32 168
Cuts at: 4430 4462 4890 4922 6685 6717 6759 6791 1373
Size: 32 428 32 1763 32 42 32 2626
Fragments arranged by size:
2626 1763 1275 664 482 428 168 135
60 42 32 32 32 32 32 32
32 32 32 32 31 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2518 4335 5388 5634 669
Size: 988 281 580 1817 1053 246 3079
Fragments arranged by size:
3079 1817 1053 988 580 281 246
HindIII A'AGCT_T
Cuts at: 748 2869 2975 748
Size: 2121 106 5817
Fragments arranged by size:
5817 2121 106
HpaI GTT'AAC
Cuts at: 5634 5634
Size: 8044
KasI G'GCGC_C
Cuts at: 4289 4289
Size: 8044
KpnI G_GTAC'C
Cuts at: 4651 5369 4651
Size: 718 7326
MfeI C'AATT_G
Cuts at: 5015 5643 5015
Size: 628 7416
MluI A'CGCG_T
Cuts at: 7920 7920
Size: 8044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 4437 7158 7342 1305
Size: 880 2252 2721 184 2007
Fragments arranged by size:
2721 2252 2007 880 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2972 3524 10
Size: 54 1425 343 1140 552 4530
Fragments arranged by size:
4530 1425 1140 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3513 4175 4709 5178 6002 6361 6520
Size: 648 2347 662 534 469 824 359 159
Cuts at: 6520 518
Size: 2042
Fragments arranged by size:
2347 2042 824 662 648 534 469 359
159
MspA1I CmG'CkG
Cuts at: 1606 2103 2116 2968 3112 3271 3421 3805 4048
Size: 497 13 852 144 159 150 384 243
Cuts at: 4048 4527 5059 5213 5460 6137 7285 7530 1606
Size: 479 532 154 247 677 1148 245 2120
Fragments arranged by size:
2120 1148 852 677 532 497 479 384
247 245 243 159 154 150 144 13
NaeI GCC'GGC
Cuts at: 4826 4826
Size: 8044
NarI GG'CG_CC
Cuts at: 4290 4290
Size: 8044
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7492
NdeI CA'TA_TG
Cuts at: 387 2191 2286 387
Size: 1804 95 6145
Fragments arranged by size:
6145 1804 95
NgoMIV G'CCGG_C
Cuts at: 4824 4824
Size: 8044
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8044
NotI GC'GGCC_GC
Cuts at: 5491 5491
Size: 8044
NspI r_CATG'y
Cuts at: 1132 1229 2402 3295 5402 6947 1132
Size: 97 1173 893 2107 1545 2229
Fragments arranged by size:
2229 2107 1545 1173 893 97
PasI CC'CwG_GG
Cuts at: 1348 3094 3829 4747 1348
Size: 1746 735 918 4645
Fragments arranged by size:
4645 1746 918 735
PciI A'CATG_T
Cuts at: 6943 6943
Size: 8044
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2103 2204 1108
Size: 995 101 6948
Fragments arranged by size:
6948 995 101
PfoI T'CCnGG_A
Cuts at: 3459 3459
Size: 8044
PmeI GTTT'AAAC
Cuts at: 5348 5348
Size: 8044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 2546 2555 2578 6113 6145 7626 7658 2523
Size: 23 9 23 3535 32 1481 32 2909
Fragments arranged by size:
3535 2909 1481 32 32 23 23 9
PpuMI rG'GwC_Cy
Cuts at: 3142 4802 3142
Size: 1660 6384
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8044
PsiI TTA'TAA
Cuts at: 5614 5614
Size: 8044
PspXI vC'TCGA_Gb
Cuts at: 2256 2256
Size: 8044
PstI C_TGCA'G
Cuts at: 830 2562 3150 3438 3561 4992 5335 5396 830
Size: 1732 588 288 123 1431 343 61 3478
Fragments arranged by size:
3478 1732 1431 588 343 288 123 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8044
PvuII CAG'CTG
Cuts at: 2103 2968 3112 3271 3421 3805 4048 4527 5059
Size: 865 144 159 150 384 243 479 532
Cuts at: 5059 5213 2103
Size: 154 4934
Fragments arranged by size:
4934 865 532 479 384 243 159 154
150 144
SacI G_AGCT'C
Cuts at: 2849 4392 5049 2849
Size: 1543 657 5844
Fragments arranged by size:
5844 1543 657
SacII CC_GC'GG
Cuts at: 2117 2117
Size: 8044
SalI G'TCGA_C
Cuts at: 1936 4333 5386 1936
Size: 2397 1053 4594
Fragments arranged by size:
4594 2397 1053
SbfI CC_TGCA'GG
Cuts at: 3150 3561 4992 5396 3150
Size: 411 1431 404 5798
Fragments arranged by size:
5798 1431 411 404
ScaI AGT'ACT
Cuts at: 6292 8004 6292
Size: 1712 6332
SexAI A'CCwGG_T
Cuts at: 2301 2346 2301
Size: 45 7999
SfcI C'TryA_G
Cuts at: 826 1045 2558 3146 3434 3557 4988 5331 5392
Size: 219 1513 588 288 123 1431 343 61
Cuts at: 5392 6527 7208 7399 826
Size: 1135 681 191 1471
Fragments arranged by size:
1513 1471 1431 1135 681 588 343 288
219 191 123 61
SfoI GGC'GCC
Cuts at: 4291 4291
Size: 8044
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8044
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8044
SmaI CCC'GGG
Cuts at: 1872 2513 3158 5371 1872
Size: 641 645 2213 4545
Fragments arranged by size:
4545 2213 645 641
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2256 2656 3283 4234 4391 5048
Size: 197 926 313 400 627 951 157 657
Cuts at: 5048 6148 7049 7311 820
Size: 1100 901 262 1553
Fragments arranged by size:
1553 1100 951 926 901 657 627 400
313 262 197 157
SnaBI TAC'GTA
Cuts at: 493 5835 493
Size: 5342 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8044
SphI G_CATG'C
Cuts at: 1132 2402 3295 5402 1132
Size: 1270 893 2107 3774
Fragments arranged by size:
3774 2107 1270 893
SspI AAT'ATT
Cuts at: 5 52 4035 5968 5
Size: 47 3983 1933 2081
Fragments arranged by size:
3983 2081 1933 47
StuI AGG'CCT
Cuts at: 1517 2990 1517
Size: 1473 6571
StyI C'CwwG_G
Cuts at: 513 1065 3696 3745 5217 5483 513
Size: 552 2631 49 1472 266 3074
Fragments arranged by size:
3074 2631 1472 552 266 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3179 3917 3965 4918 5178 6071 6088
Size: 1255 1599 738 48 953 260 893 17
Cuts at: 6088 6241 6426 6871 325
Size: 153 185 445 1498
Fragments arranged by size:
1599 1498 1255 953 893 738 445 260
185 153 48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2260 6290 8002
Size: 274 80 33 51 1443 283 4030 1712
Cuts at: 8002 96
Size: 138
Fragments arranged by size:
4030 1712 1443 283 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4828 4860 8004 8036 4828
Size: 32 3144 32 4836
Fragments arranged by size:
4836 3144 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8044
XbaI T'CTAG_A
Cuts at: 5380 5380
Size: 8044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3247 3247
Size: 8044
XhoI C'TCGA_G
Cuts at: 1943 2256 1943
Size: 313 7731
XmaI C'CCGG_G
Cuts at: 1870 2511 3156 5369 1870
Size: 641 645 2213 4545
Fragments arranged by size:
4545 2213 645 641
XmnI GAAnn'nnTTC
Cuts at: 6173 6173
Size: 8044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5341 5852 276
Size: 53 83 186 936 3807 511 2468
Fragments arranged by size:
3807 2468 936 511 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PsrI RsrII SanDI SapI SfiI
SrfI SwaI