Restriction map for
pFN21AA0866
(Circular) (Six-base) MAPSORT of: pFN21AA0866.seq Check: 3967 from: 1 to: 10621
pFN21AA0866 10621 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2403 4531 5773 7564 2403
Size: 2128 1242 1791 5460
Fragments arranged by size:
5460 2128 1791 1242
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2760 8431 278
Size: 53 83 186 936 1224 5671 2468
Fragments arranged by size:
5671 2468 1224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2421 6008 7964 1937
Size: 484 3587 1956 4594
Fragments arranged by size:
4594 3587 1956 484
Acc65I G'GTAC_C
Cuts at: 7942 7942
Size: 10621
AclI AA'CG_TT
Cuts at: 1563 8748 9121 1563
Size: 7185 373 3063
Fragments arranged by size:
7185 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5354 5561 6263 6881 7556 7577 8684 10068
Size: 3722 207 702 618 675 21 1107 1384
Cuts at: 10068 10336 1632
Size: 268 1917
Fragments arranged by size:
3722 1917 1384 1107 702 675 618 268
207 21
AflII C'TTAA_G
Cuts at: 820 1017 4628 820
Size: 197 3611 6813
Fragments arranged by size:
6813 3611 197
AflIII A'CryG_T
Cuts at: 1203 2424 6003 9520 10497 1203
Size: 1221 3579 3517 977 1327
Fragments arranged by size:
3579 3517 1327 1221 977
AgeI A'CCGG_T
Cuts at: 9452 9452
Size: 10621
AhdI GACnn_n'nnGTC
Cuts at: 3112 5935 9350 3112
Size: 2823 3415 4383
Fragments arranged by size:
4383 3415 2823
AleI CACnn'nnGTG
Cuts at: 1166 3320 4523 1166
Size: 2154 1203 7264
Fragments arranged by size:
7264 2154 1203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4130 6168 7727 1184
Size: 2946 2038 1559 4078
Fragments arranged by size:
4078 2946 2038 1559
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2702 2734 3608 3640 4995 5027 2702
Size: 32 874 32 1355 32 8296
Fragments arranged by size:
8296 1355 874 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2587 4441 4721 4762 4861 4909 5161 5689 5794
Size: 1854 280 41 99 48 252 528 105
Cuts at: 5794 5971 6088 6289 8364 9936 2587
Size: 177 117 201 2075 1572 3272
Fragments arranged by size:
3272 2075 1854 1572 528 280 252 201
177 117 105 99 48 41
ApaI G_GGCC'C
Cuts at: 5827 6478 6613 5827
Size: 651 135 9835
Fragments arranged by size:
9835 651 135
ApaLI G'TGCA_C
Cuts at: 3664 8678 9834 3664
Size: 5014 1156 4451
Fragments arranged by size:
5014 4451 1156
ApoI r'AATT_y
Cuts at: 1493 2735 6359 7930 8156 8308 10417 1493
Size: 1242 3624 1571 226 152 2109 1697
Fragments arranged by size:
3624 2109 1697 1571 1242 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3165 9175 160
Size: 872 2133 6010 1606
Fragments arranged by size:
6010 2133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10621
AvaI C'yCGr_G
Cuts at: 1870 1943 5826 6471 6509 6750 7127 7373 7884
Size: 73 3883 645 38 241 377 246 511
Cuts at: 7884 7946 1870
Size: 62 4545
Fragments arranged by size:
4545 3883 645 511 377 246 241 73
62 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2614 2647 2614
Size: 33 10588
BamHI G'GATC_C
Cuts at: 3372 7951 8318 3372
Size: 4579 367 5675
Fragments arranged by size:
5675 4579 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7942 9397 618
Size: 325 217 627 6155 1455 1842
Fragments arranged by size:
6155 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3567 3917 5247 5508 5628 5827 5838 6478
Size: 2176 350 1330 261 120 199 11 640
Cuts at: 6478 6613 7111 7395 7940 1391
Size: 135 498 284 545 4072
Fragments arranged by size:
4072 2176 1330 640 545 498 350 284
261 199 135 120 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3076 4156 4598 4815 5478 6552 6644
Size: 971 1177 1080 442 217 663 1074 92
Cuts at: 6644 6828 928
Size: 184 4721
Fragments arranged by size:
4721 1177 1080 1074 971 663 442 217
184 92
BbvCI CC'TCA_GC
Cuts at: 4722 4932 6426 4722
Size: 210 1494 8917
Fragments arranged by size:
8917 1494 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2734 2768 8812 8846 1903
Size: 34 797 34 6044 34 3678
Fragments arranged by size:
6044 3678 797 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3303 8513 9723 3303
Size: 5210 1210 4201
Fragments arranged by size:
5210 4201 1210
BclI T'GATC_A
Cuts at: 1548 4254 4978 6364 1548
Size: 2706 724 1386 5805
Fragments arranged by size:
5805 2706 1386 724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2597 4143 4385
Size: 107 122 71 631 605 925 1546 242
Cuts at: 4385 4415 9232 136
Size: 30 4817 1525
Fragments arranged by size:
4817 1546 1525 925 631 605 242 122
107 71 30
BglII A'GATC_T
Cuts at: 3396 7014 10616 3396
Size: 3618 3602 3401
Fragments arranged by size:
3618 3602 3401
BlpI GC'TnA_GC
Cuts at: 7310 8038 7310
Size: 728 9893
Bme1580I G_kGCm'C
Cuts at: 1790 2952 3668 5827 6478 6613 6844 7363 8682
Size: 1162 716 2159 651 135 231 519 1319
Cuts at: 8682 9838 1790
Size: 1156 2573
Fragments arranged by size:
2573 2159 1319 1162 1156 716 651 519
231 135
BmgBI CAC'GTC
Cuts at: 1318 2286 3868 5932 6571 1318
Size: 968 1582 2064 639 5368
Fragments arranged by size:
5368 2064 1582 968 639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2069 2101 8352
Size: 416 53 83 399 24 652 32 6251
Cuts at: 8352 9310 442
Size: 958 1753
Fragments arranged by size:
6251 1753 958 652 416 399 83 53
32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10621
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3427 3427
Size: 10621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2565 2699 2892 3506 3573 4112 4784 4817
Size: 1326 134 193 614 67 539 672 33
Cuts at: 4817 4901 4973 5183 5204 5210 5357 5372 5468
Size: 84 72 210 21 6 147 15 96
Cuts at: 5468 5711 5795 5939 6047 6110 6614 6635 6677
Size: 243 84 144 108 63 504 21 42
Cuts at: 6677 6911 6988 7169 7226 7247 7259 7481 7682
Size: 234 77 181 57 21 12 222 201
Cuts at: 7682 7851 9281 1239
Size: 169 1430 2579
Fragments arranged by size:
2579 1430 1326 672 614 539 504 243
234 222 210 201 193 181 169 147
144 134 108 96 84 84 77 72
67 63 57 42 33 21 21 21
15 12 6
Bpu10I CC'TnA_GC
Cuts at: 4722 4932 5507 5580 6426 7787 4722
Size: 210 575 73 846 1361 7556
Fragments arranged by size:
7556 1361 846 575 210 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3695 4469 4937 5036 5231 5660 6704 7634 7760
Size: 774 468 99 195 429 1044 930 126
Cuts at: 7760 8746 9611 9909 3695
Size: 986 865 298 4407
Fragments arranged by size:
4407 1044 986 930 865 774 468 429
298 195 126 99
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2783 5424 6611 7796 9284 882
Size: 617 1284 2641 1187 1185 1488 2219
Fragments arranged by size:
2641 2219 1488 1284 1187 1185 617
BsaAI yAC'GTr
Cuts at: 493 1204 2352 8412 493
Size: 711 1148 6060 2702
Fragments arranged by size:
6060 2702 1148 711
BsaBI GATnn'nnATC
Cuts at: 10615 10615
Size: 10621
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2757 3113 4323 5936
Size: 53 83 186 936 1224 356 1210 1613
Cuts at: 5936 6684 8428 8810 9445 275
Size: 748 1744 382 635 1451
Fragments arranged by size:
1744 1613 1451 1224 1210 936 748 635
382 356 186 83 53
BsaWI w'CCGG_w
Cuts at: 2490 4155 7395 9054 9452 9726 9873 10284 2490
Size: 1665 3240 1659 398 274 147 411 2827
Fragments arranged by size:
3240 2827 1665 1659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2285 2300 2315 2504
Size: 30 165 30 867 15 15 15 189
Cuts at: 2504 2534 2702 2732 3647 3677 4995 5025 5783
Size: 30 168 30 915 30 1318 30 758
Cuts at: 5783 5813 1178
Size: 30 5986
Fragments arranged by size:
5986 1318 915 867 758 189 168 165
30 30 30 30 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2593 2710 3038 3041 3189 3509 3823
Size: 988 419 117 328 3 148 320 314
Cuts at: 3823 4355 4358 4571 4574 4679 4739 4781 4814
Size: 532 3 213 3 105 60 42 33
Cuts at: 4814 4817 4898 4901 5033 5285 5288 5456 5636
Size: 3 81 3 132 252 3 168 180
Cuts at: 5636 6044 6047 6080 6245 6395 6644 6782 6790
Size: 408 3 33 165 150 249 138 8
Cuts at: 6790 7163 7244 7247 7256 7259 7499 7679 7688
Size: 373 81 3 9 3 240 180 9
Cuts at: 7688 7691 7883 1186
Size: 3 192 3924
Fragments arranged by size:
3924 988 532 419 408 373 328 320
314 252 249 240 213 192 180 180
168 165 150 148 138 132 117 105
81 81 60 42 33 33 9 9
8 3 3 3 3 3 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2956 3746 3923 3985 4851 5949 6219 7221 7683
Size: 790 177 62 866 1098 270 1002 462
Cuts at: 7683 9824 10320 2956
Size: 2141 496 3257
Fragments arranged by size:
3257 2141 1098 1002 866 790 496 462
270 177 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5807 5827 1163
Size: 573 4071 20 5957
Fragments arranged by size:
5957 4071 573 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3578 6243 7276 8071 8832 9860
Size: 275 186 1577 2665 1033 795 761 1028
Cuts at: 9860 1540
Size: 2301
Fragments arranged by size:
2665 2301 1577 1033 1028 795 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 3567 3668 3917 5247 5508 5628 5838 7395 8682
Size: 101 249 1330 261 120 210 1557 1287
Cuts at: 8682 8767 9838 3567
Size: 85 1071 4350
Fragments arranged by size:
4350 1557 1330 1287 1071 261 249 210
120 101 85
BsmI GAATG_Cn'
Cuts at: 3623 4125 4210 4245 8132 8225 3623
Size: 502 85 35 3887 93 6019
Fragments arranged by size:
6019 3887 502 93 85 35
BsmBI CGTCTCn'nnnn_
Cuts at: 2826 5555 6803 7881 2826
Size: 2729 1248 1078 5566
Fragments arranged by size:
5566 2729 1248 1078
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2952 3567 3668 3917 5247 5508 5628
Size: 399 1162 615 101 249 1330 261 120
Cuts at: 5628 5827 5838 6478 6613 6844 7111 7363 7395
Size: 199 11 640 135 231 267 252 32
Cuts at: 7395 7940 8682 8767 9838 1391
Size: 545 742 85 1071 2174
Fragments arranged by size:
2174 1330 1162 1071 742 640 615 545
399 267 261 252 249 231 199 135
120 101 85 32 11
BspEI T'CCGG_A
Cuts at: 7395 10284 7395
Size: 2889 7732
BspHI T'CATG_A
Cuts at: 4251 8510 10240 4251
Size: 4259 1730 4632
Fragments arranged by size:
4632 4259 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 4531 4683 5773 7480 7564 7976 844
Size: 1559 2128 152 1090 1707 84 412 3489
Fragments arranged by size:
3489 2128 1707 1559 1090 412 152 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2326 4603 5395 5821 6037 6514 7378
Size: 429 503 2277 792 426 216 477 864
Cuts at: 7378 8508 1394
Size: 1130 3507
Fragments arranged by size:
3507 2277 1130 864 792 503 477 429
426 216
BsrDI GCAATG_nn'
Cuts at: 65 1248 4114 7636 9116 9290 65
Size: 1183 2866 3522 1480 174 1396
Fragments arranged by size:
3522 2866 1480 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4137 9265 9452 1700
Size: 110 2327 5128 187 2869
Fragments arranged by size:
5128 2869 2327 187 110
BsrGI T'GTAC_A
Cuts at: 96 2427 3972 96
Size: 2331 1545 6745
Fragments arranged by size:
6745 2331 1545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10621
BssSI C'ACGA_G
Cuts at: 2447 4052 5207 6329 6800 7409 8681 9693 2447
Size: 1605 1155 1122 471 609 1272 1012 3375
Fragments arranged by size:
3375 1605 1272 1155 1122 1012 609 471
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2452 4909 5794 794
Size: 1658 2457 885 5621
Fragments arranged by size:
5621 2457 1658 885
BstBI TT'CG_AA
Cuts at: 6732 9425 6732
Size: 2693 7928
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10621
BstXI CCAn_nnnn'nTGG
Cuts at: 3746 3790 5920 5932 6163 7057 3746
Size: 44 2130 12 231 894 7310
Fragments arranged by size:
7310 2130 894 231 44 12
BstYI r'GATC_y
Cuts at: 1972 3185 3372 3396 4191 4962 5340 6739 7014
Size: 1213 187 24 795 771 378 1399 275
Cuts at: 7014 7951 8318 8703 8720 10172 10258 10270 10616
Size: 937 367 385 17 1452 86 12 346
Cuts at: 10616 1972
Size: 1977
Fragments arranged by size:
1977 1452 1399 1213 937 795 771 385
378 367 346 275 187 86 24 17
12
Bsu36I CC'TnA_GG
Cuts at: 2318 2318
Size: 10621
BtgI C'CryG_G
Cuts at: 513 1065 2004 2592 2991 3000 3636 6162 6675
Size: 552 939 588 399 9 636 2526 513
Cuts at: 6675 7734 7752 513
Size: 1059 18 3382
Fragments arranged by size:
3382 2526 1059 939 636 588 552 513
399 18 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2458 4403 5826 7435 487
Size: 683 56 1232 1945 1423 1609 3673
Fragments arranged by size:
3673 1945 1609 1423 1232 683 56
BtsI GCAGTG_nn'
Cuts at: 4508 4532 8137 8931 8951 4508
Size: 24 3605 794 20 6178
Fragments arranged by size:
6178 3605 794 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10621
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10586
DraI TTT'AAA
Cuts at: 7925 7925
Size: 10621
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9628 809
Size: 507 8312 1802
Fragments arranged by size:
8312 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 3276 3849
Size: 54 1425 50 275 18 759 687 573
Cuts at: 3849 3897 4407 4746 5259 5292 5712 5922 6240
Size: 48 510 339 513 33 420 210 318
Cuts at: 6240 7155 7323 7521 7596 8068 8957 10586 8
Size: 915 168 198 75 472 889 1629 43
Fragments arranged by size:
1629 1425 915 889 759 687 573 513
510 472 420 339 318 275 210 198
168 75 54 50 48 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 6240 8068 1537
Size: 275 4428 1828 4090
Fragments arranged by size:
4428 4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 2435 3467 4052 5049 5136 5141 5553
Size: 1016 66 1032 585 997 87 5 412
Cuts at: 5553 6171 6182 6400 6477 6645 6767 8551 1353
Size: 618 11 218 77 168 122 1784 3423
Fragments arranged by size:
3423 1784 1032 1016 997 618 585 412
218 168 122 87 77 66 11 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2291 4166 5846 9208 9582 9728
Size: 1064 138 702 1875 1680 3362 374 146
Cuts at: 9728 387
Size: 1280
Fragments arranged by size:
3362 1875 1680 1280 1064 702 374 146
138
EcoICRI GAG'CTC
Cuts at: 3565 3915 5245 5506 5626 5836 7393 3565
Size: 350 1330 261 120 210 1557 6793
Fragments arranged by size:
6793 1557 1330 350 261 210 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2565 2699 2892 3506 3573 4112 4784
Size: 393 933 134 193 614 67 539 672
Cuts at: 4784 4817 4901 4973 5183 5204 5210 5354 5357
Size: 33 84 72 210 21 6 144 3
Cuts at: 5357 5372 5468 5561 5711 5795 5939 6047 6110
Size: 15 96 93 150 84 144 108 63
Cuts at: 6110 6263 6614 6635 6677 6881 6911 6988 7169
Size: 153 351 21 42 204 30 77 181
Cuts at: 7169 7226 7247 7259 7481 7556 7577 7682 7851
Size: 57 21 12 222 75 21 105 169
Cuts at: 7851 8684 9281 10068 10336 1239
Size: 833 597 787 268 1524
Fragments arranged by size:
1524 933 833 787 672 614 597 539
393 351 268 222 210 204 193 181
169 153 150 144 144 134 108 105
96 93 84 84 77 75 72 67
63 57 42 33 30 21 21 21
21 15 12 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3488 3860 4340 4679 4799 5375 5438 5735 6482
Size: 372 480 339 120 576 63 297 747
Cuts at: 6482 7130 3488
Size: 648 6979
Fragments arranged by size:
6979 747 648 576 480 372 339 297
120 63
EcoO109I rG'GnC_Cy
Cuts at: 2116 5380 5452 5548 5728 6457 6475 6610 6877
Size: 3264 72 96 180 729 18 135 267
Cuts at: 6877 7087 2116
Size: 210 5650
Fragments arranged by size:
5650 3264 729 267 210 180 135 96
72 18
EcoRI G'AATT_C
Cuts at: 7930 8308 7930
Size: 378 10243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2152 7582 2062
Size: 90 5430 5101
Fragments arranged by size:
5430 5101 90
FspI TGC'GCA
Cuts at: 9127 9127
Size: 10621
HaeII r_GCGC'y
Cuts at: 1678 3298 3930 6097 6719 7201 7460 9768 1678
Size: 1620 632 2167 622 482 259 2308 2531
Fragments arranged by size:
2531 2308 2167 1620 632 622 482 259
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2443 2475 2504 2536 2705
Size: 32 135 32 871 32 29 32 169
Cuts at: 2705 2737 3020 3052 3098 3130 3177 3209 3381
Size: 32 283 32 46 32 47 32 172
Cuts at: 3381 3413 4046 4078 5372 5404 5552 5584 5783
Size: 32 633 32 1294 32 148 32 199
Cuts at: 5783 5815 5921 5953 6999 7031 7664 7696 9262
Size: 32 106 32 1046 32 633 32 1566
Cuts at: 9262 9294 9336 9368 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 1294 1046 871 633 633 283
199 172 169 148 135 106 47 46
42 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2380 2422 6706 7965 8211 669
Size: 988 281 442 42 4284 1259 246 3079
Fragments arranged by size:
4284 3079 1259 988 442 281 246 42
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10621
HpaI GTT'AAC
Cuts at: 8211 8211
Size: 10621
KpnI G_GTAC'C
Cuts at: 7946 7946
Size: 10621
MfeI C'AATT_G
Cuts at: 8220 8220
Size: 10621
MluI A'CGCG_T
Cuts at: 10497 10497
Size: 10621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3861 6150 6360 9735 9919 1305
Size: 2556 2289 210 3375 184 2007
Fragments arranged by size:
3375 2556 2289 2007 210 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3278 3899 4409 5261 5294
Size: 54 1425 343 1446 621 510 852 33
Cuts at: 5294 5924 7157 7325 10
Size: 630 1233 168 3306
Fragments arranged by size:
3306 1446 1425 1233 852 630 621 510
343 168 54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 3320 4250 4454 4523 5885 6804 8579
Size: 648 2154 930 204 69 1362 919 1775
Cuts at: 8579 8938 9097 518
Size: 359 159 2042
Fragments arranged by size:
2154 2042 1775 1362 930 919 648 359
204 159 69
MspA1I CmG'CkG
Cuts at: 1606 3134 3306 3424 3799 3970 3985 4099 4441
Size: 1528 172 118 375 171 15 114 342
Cuts at: 4441 4657 4690 4879 5257 6025 6058 6322 6646
Size: 216 33 189 378 768 33 264 324
Cuts at: 6646 6851 7100 7225 7660 7717 8037 8714 9862
Size: 205 249 125 435 57 320 677 1148
Cuts at: 9862 10107 1606
Size: 245 2120
Fragments arranged by size:
2120 1528 1148 768 677 435 378 375
342 324 320 264 249 245 216 205
189 172 171 125 118 114 57 33
33 15
NaeI GCC'GGC
Cuts at: 4139 4139
Size: 10621
NcoI C'CATG_G
Cuts at: 513 1065 2004 3000 7752 513
Size: 552 939 996 4752 3382
Fragments arranged by size:
4752 3382 996 939 552
NdeI CA'TA_TG
Cuts at: 387 3289 387
Size: 2902 7719
NgoMIV G'CCGG_C
Cuts at: 4137 4137
Size: 10621
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10621
NotI GC'GGCC_GC
Cuts at: 6240 8068 6240
Size: 1828 8793
NspI r_CATG'y
Cuts at: 1132 1229 2428 2500 3952 6712 7979 9524 1132
Size: 97 1199 72 1452 2760 1267 1545 2229
Fragments arranged by size:
2760 2229 1545 1452 1267 1199 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10621
PciI A'CATG_T
Cuts at: 2424 9520 2424
Size: 7096 3525
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3157 6592 7414 1108
Size: 2049 3435 822 4315
Fragments arranged by size:
4315 3435 2049 822
PfoI T'CCnGG_A
Cuts at: 2982 3375 3483 4167 5343 5373 5586 5955 7185
Size: 393 108 684 1176 30 213 369 1230
Cuts at: 7185 2982
Size: 6418
Fragments arranged by size:
6418 1230 1176 684 393 369 213 108
30
PmeI GTTT'AAAC
Cuts at: 7925 7925
Size: 10621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2702 2734 4995 5027 8690 8722 10203 10235 2702
Size: 32 2261 32 3663 32 1481 32 3088
Fragments arranged by size:
3663 3088 2261 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 5380 5728 6877 7087 2116
Size: 3264 348 1149 210 5650
Fragments arranged by size:
5650 3264 1149 348 210
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10621
PsiI TTA'TAA
Cuts at: 8191 8191
Size: 10621
PspOMI G'GGCC_C
Cuts at: 5823 6474 6609 5823
Size: 651 135 9835
Fragments arranged by size:
9835 651 135
PspXI vC'TCGA_Gb
Cuts at: 6509 7373 6509
Size: 864 9757
PstI C_TGCA'G
Cuts at: 830 3069 3456 4542 4590 4695 5283 5799 5862
Size: 2239 387 1086 48 105 588 516 63
Cuts at: 5862 6147 6789 6856 6957 7575 7716 7973 830
Size: 285 642 67 101 618 141 257 3478
Fragments arranged by size:
3478 2239 1086 642 618 588 516 387
285 257 141 105 101 67 63 48
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10621
PvuII CAG'CTG
Cuts at: 3134 3424 3970 3985 4099 4441 4657 4690 4879
Size: 290 546 15 114 342 216 33 189
Cuts at: 4879 5257 6025 6058 6646 6851 7225 7660 3134
Size: 378 768 33 588 205 374 435 6095
Fragments arranged by size:
6095 768 588 546 435 378 374 342
290 216 205 189 114 33 33 15
SacI G_AGCT'C
Cuts at: 3567 3917 5247 5508 5628 5838 7395 3567
Size: 350 1330 261 120 210 1557 6793
Fragments arranged by size:
6793 1557 1330 350 261 210 120
SalI G'TCGA_C
Cuts at: 1936 2420 7963 1936
Size: 484 5543 4594
Fragments arranged by size:
5543 4594 484
SanDI GG'GwC_CC
Cuts at: 2116 2116
Size: 10621
SapI GCTCTTCn'nnn_
Cuts at: 3467 5141 6645 3467
Size: 1674 1504 7443
Fragments arranged by size:
7443 1674 1504
SbfI CC_TGCA'GG
Cuts at: 7973 7973
Size: 10621
ScaI AGT'ACT
Cuts at: 8869 10581 8869
Size: 1712 8909
SexAI A'CCwGG_T
Cuts at: 6297 6549 6297
Size: 252 10369
SfcI C'TryA_G
Cuts at: 826 1045 3065 3264 3452 3548 4538 4586 4679
Size: 219 2020 199 188 96 990 48 93
Cuts at: 4679 4691 4826 5279 5795 5858 6143 6785 6852
Size: 12 135 453 516 63 285 642 67
Cuts at: 6852 6953 7571 7712 7969 9104 9785 9976 826
Size: 101 618 141 257 1135 681 191 1471
Fragments arranged by size:
2020 1471 1135 990 681 642 618 516
453 285 257 219 199 191 188 141
135 101 96 93 67 63 48 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2597 2597
Size: 10621
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10621
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10621
SmaI CCC'GGG
Cuts at: 1872 5828 6752 7948 1872
Size: 3956 924 1196 4545
Fragments arranged by size:
4545 3956 1196 924
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3710 4484 4628 4916 5015 5246
Size: 197 926 1767 774 144 288 99 231
Cuts at: 5246 5639 6509 6719 7127 7373 7649 7775 7884
Size: 393 870 210 408 246 276 126 109
Cuts at: 7884 8725 9626 9888 820
Size: 841 901 262 1553
Fragments arranged by size:
1767 1553 926 901 870 841 774 408
393 288 276 262 246 231 210 197
144 126 109 99
SnaBI TAC'GTA
Cuts at: 493 2352 8412 493
Size: 1859 6060 2702
Fragments arranged by size:
6060 2702 1859
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10621
SphI G_CATG'C
Cuts at: 1132 2500 7979 1132
Size: 1368 5479 3774
Fragments arranged by size:
5479 3774 1368
SrfI GCCC'GGGC
Cuts at: 5828 5828
Size: 10621
SspI AAT'ATT
Cuts at: 5 52 8545 5
Size: 47 8493 2081
Fragments arranged by size:
8493 2081 47
StuI AGG'CCT
Cuts at: 1517 4241 6488 1517
Size: 2724 2247 5650
Fragments arranged by size:
5650 2724 2247
StyI C'CwwG_G
Cuts at: 513 1065 2004 2259 2877 3000 3279 4572 4596
Size: 552 939 255 618 123 279 1293 24
Cuts at: 4596 5658 5925 6453 6489 6561 7752 7908 8060
Size: 1062 267 528 36 72 1191 156 152
Cuts at: 8060 513
Size: 3074
Fragments arranged by size:
3074 1293 1191 1062 939 618 552 528
279 267 255 156 152 123 72 36
24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2263 3049 3592 4676 5978 6233 8648
Size: 1255 683 786 543 1084 1302 255 2415
Cuts at: 8648 8665 8818 9003 9448 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2415 1498 1302 1255 1084 786 683 543
445 255 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 3972 7605
Size: 274 80 33 51 1443 450 1545 3633
Cuts at: 7605 8867 10579 96
Size: 1262 1712 138
Fragments arranged by size:
3633 1712 1545 1443 1262 450 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 3153 3185 3647 3679 10581 10613 2270
Size: 32 851 32 462 32 6902 32 2278
Fragments arranged by size:
6902 2278 851 462 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5566 5846 6551 7277 1316
Size: 4250 280 705 726 4660
Fragments arranged by size:
4660 4250 726 705 280
XbaI T'CTAG_A
Cuts at: 7820 7957 7820
Size: 137 10484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 3046 3481 4222 5749 6085 6568 6610 6694
Size: 39 435 741 1527 336 483 42 84
Cuts at: 6694 7456 3007
Size: 762 6172
Fragments arranged by size:
6172 1527 762 741 483 435 336 84
42 39
XhoI C'TCGA_G
Cuts at: 1943 6509 7127 7373 7884 1943
Size: 4566 618 246 511 4680
Fragments arranged by size:
4680 4566 618 511 246
XmaI C'CCGG_G
Cuts at: 1870 5826 6750 7946 1870
Size: 3956 924 1196 4545
Fragments arranged by size:
4545 3956 1196 924
XmnI GAAnn'nnTTC
Cuts at: 3413 4125 6191 8750 3413
Size: 712 2066 2559 5284
Fragments arranged by size:
5284 2559 2066 712
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2758 8429 276
Size: 53 83 186 936 1224 5671 2468
Fragments arranged by size:
5671 2468 1224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PsrI RsrII SacII SfoI SwaI