Restriction map for
pFN21AA0852
(Circular) (Six-base) MAPSORT of: pFN21AA0852.seq Check: 1826 from: 1 to: 7615
pFN21AA0852 7615 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3141 3141
Size: 7615
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2504 5425 278
Size: 53 83 186 936 968 2921 2468
Fragments arranged by size:
2921 2468 968 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2099 3643 4958 1937
Size: 162 1544 1315 4594
Fragments arranged by size:
4594 1544 1315 162
Acc65I G'GTAC_C
Cuts at: 4936 4936
Size: 7615
AclI AA'CG_TT
Cuts at: 1563 5742 6115 1563
Size: 4179 373 3063
Fragments arranged by size:
4179 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2745 3536 3608 4679 5678 7062 7330 1632
Size: 1113 791 72 1071 999 1384 268 1917
Fragments arranged by size:
1917 1384 1113 1071 999 791 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7418
AflIII A'CryG_T
Cuts at: 1203 2655 6514 7491 1203
Size: 1452 3859 977 1327
Fragments arranged by size:
3859 1452 1327 977
AgeI A'CCGG_T
Cuts at: 3420 6446 3420
Size: 3026 4589
AhdI GACnn_n'nnGTC
Cuts at: 6344 6344
Size: 7615
AleI CACnn'nnGTG
Cuts at: 1166 4259 1166
Size: 3093 4522
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4638 1184
Size: 3454 4161
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 7583
AlwNI CAG_nnn'CTG
Cuts at: 3901 4046 4807 5358 6930 3901
Size: 145 761 551 1572 4586
Fragments arranged by size:
4586 1572 761 551 145
ApaI G_GGCC'C
Cuts at: 3014 4442 3014
Size: 1428 6187
ApaLI G'TGCA_C
Cuts at: 5672 6828 5672
Size: 1156 6459
ApoI r'AATT_y
Cuts at: 1493 2309 2917 3519 4630 4924 5150 5302 7411
Size: 816 608 602 1111 294 226 152 2109
Cuts at: 7411 1493
Size: 1697
Fragments arranged by size:
2109 1697 1111 816 608 602 294 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6169 160
Size: 872 5137 1606
Fragments arranged by size:
5137 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7615
AvaI C'yCGr_G
Cuts at: 1870 1943 2280 3792 3936 4731 4940 1870
Size: 73 337 1512 144 795 209 4545
Fragments arranged by size:
4545 1512 795 337 209 144 73
AvrII C'CTAG_G
Cuts at: 2776 2776
Size: 7615
BamHI G'GATC_C
Cuts at: 2038 4945 5312 2038
Size: 2907 367 4341
Fragments arranged by size:
4341 2907 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3129 3835 4936 6391 618
Size: 325 217 627 1342 706 1101 1455 1842
Fragments arranged by size:
1842 1455 1342 1101 706 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2141 3014 3935 4146 4192 4442 4476 4692
Size: 750 873 921 211 46 250 34 216
Cuts at: 4692 4934 1391
Size: 242 4072
Fragments arranged by size:
4072 921 873 750 250 242 216 211
46 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2190 3488 3989 928
Size: 971 291 1298 501 4554
Fragments arranged by size:
4554 1298 971 501 291
BbvCI CC'TCA_GC
Cuts at: 3648 4808 3648
Size: 1160 6455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5806 5840 1903
Size: 34 50 34 3785 34 3678
Fragments arranged by size:
3785 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5507 6717 5507
Size: 1210 6405
BclI T'GATC_A
Cuts at: 1548 2415 2988 3681 1548
Size: 867 573 693 5482
Fragments arranged by size:
5482 867 693 573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2527 4454 6226
Size: 107 122 71 631 605 855 1927 1772
Cuts at: 6226 136
Size: 1525
Fragments arranged by size:
1927 1772 1525 855 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7610 7610
Size: 7615
BlpI GC'TnA_GC
Cuts at: 5032 5032
Size: 7615
Bme1580I G_kGCm'C
Cuts at: 1790 2516 2598 3014 3069 4314 4442 5676 6832
Size: 726 82 416 55 1245 128 1234 1156
Cuts at: 6832 1790
Size: 2573
Fragments arranged by size:
2573 1245 1234 1156 726 416 128 82
55
BmgBI CAC'GTC
Cuts at: 1318 2196 2658 1318
Size: 878 462 6275
Fragments arranged by size:
6275 878 462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2371 5346 6304
Size: 416 53 83 399 24 954 2975 958
Cuts at: 6304 442
Size: 1753
Fragments arranged by size:
2975 1753 958 954 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7615
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2548 2772 2548
Size: 224 7391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2785 3101 3563 3806 3954 6275 1239
Size: 1546 316 462 243 148 2321 2579
Fragments arranged by size:
2579 2321 1546 462 316 243 148
Bpu10I CC'TnA_GC
Cuts at: 3648 4520 4808 3648
Size: 872 288 6455
Fragments arranged by size:
6455 872 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4166 5740 6605 6903 4166
Size: 1574 865 298 4878
Fragments arranged by size:
4878 1574 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2778 4326 4529 6278 882
Size: 617 1279 1548 203 1749 2219
Fragments arranged by size:
2219 1749 1548 1279 617 203
BsaAI yAC'GTr
Cuts at: 493 1204 2710 4213 4309 5406 493
Size: 711 1506 1503 96 1097 2702
Fragments arranged by size:
2702 1506 1503 1097 711 96
BsaBI GATnn'nnATC
Cuts at: 2579 2585 7609 2579
Size: 6 5024 2585
Fragments arranged by size:
5024 2585 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2501 4881 5422 5804
Size: 53 83 186 936 968 2380 541 382
Cuts at: 5804 6439 275
Size: 635 1451
Fragments arranged by size:
2380 1451 968 936 635 541 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3420 4387 4563 6048 6446 6720 6867 7278 3420
Size: 967 176 1485 398 274 147 411 3757
Fragments arranged by size:
3757 1485 967 411 398 274 176 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7390
Fragments arranged by size:
7390 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 3812 4079 4093 4442 4445 4805 1186
Size: 1189 1437 267 14 349 3 360 3996
Fragments arranged by size:
3996 1437 1189 360 349 267 14 3
BseYI C'CCAG_C
Cuts at: 3695 4157 4338 4449 6818 7314 3695
Size: 462 181 111 2369 496 3996
Fragments arranged by size:
3996 2369 496 462 181 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 3914 4193 4862 1163
Size: 573 888 1290 279 669 3916
Fragments arranged by size:
3916 1290 888 669 573 279
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3662 5065 5826 6854 1540
Size: 275 186 1661 1403 761 1028 2301
Fragments arranged by size:
2301 1661 1403 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 2709 2896 3156 4146 4192 4526 5676 5761
Size: 150 187 260 990 46 334 1150 85
Cuts at: 5761 6832 2559
Size: 1071 3342
Fragments arranged by size:
3342 1150 1071 990 334 260 187 150
85 46
BsmI GAATG_Cn'
Cuts at: 2275 4493 5126 5219 2275
Size: 2218 633 93 4671
Fragments arranged by size:
4671 2218 633 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2492 4778 2492
Size: 2286 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2516 2559 2598 2709 2896 3014
Size: 399 351 375 43 39 111 187 118
Cuts at: 3014 3069 3156 3935 4146 4192 4314 4442 4476
Size: 55 87 779 211 46 122 128 34
Cuts at: 4476 4526 4692 4934 5676 5761 6832 1391
Size: 50 166 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 779 742 399 375 351 242
211 187 166 128 122 118 111 87
85 55 50 46 43 39 34
BspEI T'CCGG_A
Cuts at: 4387 4563 7278 4387
Size: 176 2715 4724
Fragments arranged by size:
4724 2715 176
BspHI T'CATG_A
Cuts at: 2221 5504 7234 2221
Size: 3283 1730 2602
Fragments arranged by size:
3283 2602 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3141 3682 3889 4554 4970 844
Size: 2297 541 207 665 416 3489
Fragments arranged by size:
3489 2297 665 541 416 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2527 3573 5502 1394
Size: 429 704 1046 1929 3507
Fragments arranged by size:
3507 1929 1046 704 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3150 6110 6284 65
Size: 1183 1902 2960 174 1396
Fragments arranged by size:
2960 1902 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3126 3420 6259 6446 1700
Size: 110 1316 294 2839 187 2869
Fragments arranged by size:
2869 2839 1316 294 187 110
BsrGI T'GTAC_A
Cuts at: 96 2631 2646 96
Size: 2535 15 5065
Fragments arranged by size:
5065 2535 15
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7615
BssSI C'ACGA_G
Cuts at: 4532 5675 6687 4532
Size: 1143 1012 5460
Fragments arranged by size:
5460 1143 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2602 2720 4282 4840 794
Size: 1808 118 1562 558 3569
Fragments arranged by size:
3569 1808 1562 558 118
BstBI TT'CG_AA
Cuts at: 6419 6419
Size: 7615
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7615
BstXI CCAn_nnnn'nTGG
Cuts at: 4822 4822
Size: 7615
BstYI r'GATC_y
Cuts at: 1972 2038 2580 2950 4557 4945 5312 5697 5714
Size: 66 542 370 1607 388 367 385 17
Cuts at: 5714 7166 7252 7264 7610 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1607 1452 542 388 385 370 367
346 86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2907 3831 2907
Size: 924 6691
BtgI C'CryG_G
Cuts at: 513 1065 2590 4260 513
Size: 552 1525 1670 3868
Fragments arranged by size:
3868 1670 1525 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3169 487
Size: 683 56 1943 4933
Fragments arranged by size:
4933 1943 683 56
BtsI GCAGTG_nn'
Cuts at: 2255 4360 4512 5131 5925 5945 2255
Size: 2105 152 619 794 20 3925
Fragments arranged by size:
3925 2105 794 619 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7580
DraI TTT'AAA
Cuts at: 4919 4919
Size: 7615
DraIII CAC_nnn'GTG
Cuts at: 2602 2602
Size: 7615
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3651 4550 6622 809
Size: 507 2335 899 2072 1802
Fragments arranged by size:
2335 2072 1802 899 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 5062 5951
Size: 54 1425 50 275 18 249 2983 889
Cuts at: 5951 7580 8
Size: 1629 43
Fragments arranged by size:
2983 1629 1425 889 275 249 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5062 1537
Size: 275 3250 4090
Fragments arranged by size:
4090 3250 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2721 2861 3486 4158 5545 1353
Size: 854 514 140 625 672 1387 3423
Fragments arranged by size:
3423 1387 854 672 625 514 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6202 6576 6722 387
Size: 1064 138 4613 374 146 1280
Fragments arranged by size:
4613 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4144 4190 4144
Size: 46 7569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2745 2785 3101 3536 3563 3608 3806
Size: 393 1113 40 316 435 27 45 198
Cuts at: 3806 3954 4679 5678 6275 7062 7330 1239
Size: 148 725 999 597 787 268 1524
Fragments arranged by size:
1524 1113 999 787 725 597 435 393
316 268 198 148 45 40 27
EcoNI CCTnn'n_nnAGG
Cuts at: 4768 4835 4768
Size: 67 7548
EcoO109I rG'GnC_Cy
Cuts at: 3547 4439 4894 3547
Size: 892 455 6268
Fragments arranged by size:
6268 892 455
EcoRI G'AATT_C
Cuts at: 4630 4924 5302 4630
Size: 294 378 6943
Fragments arranged by size:
6943 378 294
EcoRV GAT'ATC
Cuts at: 2485 2485
Size: 7615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4684 4684
Size: 7615
FspI TGC'GCA
Cuts at: 2147 2846 4760 6121 2147
Size: 699 1914 1361 3641
Fragments arranged by size:
3641 1914 1361 699
FspAI rTGC'GCAy
Cuts at: 2147 2147
Size: 7615
HaeII r_GCGC'y
Cuts at: 1678 2533 6762 1678
Size: 855 4229 2531
Fragments arranged by size:
4229 2531 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 4513 4545 4631
Size: 32 135 32 881 32 2028 32 86
Cuts at: 4631 4663 6256 6288 6330 6362 1373
Size: 32 1593 32 42 32 2626
Fragments arranged by size:
2626 2028 1593 881 135 86 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2935 4959 5205 669
Size: 988 281 997 2024 246 3079
Fragments arranged by size:
3079 2024 997 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7615
HpaI GTT'AAC
Cuts at: 5205 5205
Size: 7615
KpnI G_GTAC'C
Cuts at: 4940 4940
Size: 7615
MfeI C'AATT_G
Cuts at: 5214 5214
Size: 7615
MluI A'CGCG_T
Cuts at: 7491 7491
Size: 7615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4004 6729 6913 1305
Size: 2699 2725 184 2007
Fragments arranged by size:
2725 2699 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5793
Fragments arranged by size:
5793 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4259 5573 5932 6091 518
Size: 648 3093 1314 359 159 2042
Fragments arranged by size:
3093 2042 1314 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2403 2552 3019 3352 4338 4618 5031 5708
Size: 797 149 467 333 986 280 413 677
Cuts at: 5708 6856 7101 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 986 797 677 467 413 333
280 245 149
NcoI C'CATG_G
Cuts at: 513 1065 2590 513
Size: 552 1525 5538
Fragments arranged by size:
5538 1525 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7615
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7615
NotI GC'GGCC_GC
Cuts at: 5062 5062
Size: 7615
NspI r_CATG'y
Cuts at: 1132 1229 2965 3039 4280 4973 6518 1132
Size: 97 1736 74 1241 693 1545 2229
Fragments arranged by size:
2229 1736 1545 1241 693 97 74
PasI CC'CwG_GG
Cuts at: 1348 3457 1348
Size: 2109 5506
PciI A'CATG_T
Cuts at: 6514 6514
Size: 7615
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7615
PfoI T'CCnGG_A
Cuts at: 3078 3078
Size: 7615
PmeI GTTT'AAAC
Cuts at: 4919 4919
Size: 7615
PmlI CAC'GTG
Cuts at: 4213 4213
Size: 7615
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5684 5716 7197 7229 5684
Size: 32 1481 32 6070
Fragments arranged by size:
6070 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4894 4894
Size: 7615
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7615
PsiI TTA'TAA
Cuts at: 5185 5185
Size: 7615
PspOMI G'GGCC_C
Cuts at: 3010 4438 3010
Size: 1428 6187
PstI C_TGCA'G
Cuts at: 830 4803 4967 830
Size: 3973 164 3478
Fragments arranged by size:
3973 3478 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7615
PvuII CAG'CTG
Cuts at: 3019 3352 4618 3019
Size: 333 1266 6016
Fragments arranged by size:
6016 1266 333
SacI G_AGCT'C
Cuts at: 4146 4192 4146
Size: 46 7569
SalI G'TCGA_C
Cuts at: 1936 4957 1936
Size: 3021 4594
SbfI CC_TGCA'GG
Cuts at: 4967 4967
Size: 7615
ScaI AGT'ACT
Cuts at: 2342 4667 5863 7575 2342
Size: 2325 1196 1712 2382
Fragments arranged by size:
2382 2325 1712 1196
SexAI A'CCwGG_T
Cuts at: 3072 3072
Size: 7615
SfcI C'TryA_G
Cuts at: 826 1045 2977 4799 4963 6098 6779 6970 826
Size: 219 1932 1822 164 1135 681 191 1471
Fragments arranged by size:
1932 1822 1471 1135 681 219 191 164
SfiI GGCCn_nnn'nGGCC
Cuts at: 4454 4454
Size: 7615
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7615
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7615
SmaI CCC'GGG
Cuts at: 1872 2282 3794 4733 4942 1872
Size: 410 1512 939 209 4545
Fragments arranged by size:
4545 1512 939 410 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4181 5719 6620 6882 820
Size: 197 926 2238 1538 901 262 1553
Fragments arranged by size:
2238 1553 1538 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5406 493
Size: 4913 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7615
SphI G_CATG'C
Cuts at: 1132 4973 1132
Size: 3841 3774
SspI AAT'ATT
Cuts at: 5 52 5539 5
Size: 47 5487 2081
Fragments arranged by size:
5487 2081 47
StuI AGG'CCT
Cuts at: 1517 4742 1517
Size: 3225 4390
StyI C'CwwG_G
Cuts at: 513 1065 2590 2776 3117 3600 4737 4905 5054
Size: 552 1525 186 341 483 1137 168 149
Cuts at: 5054 513
Size: 3074
Fragments arranged by size:
3074 1525 1137 552 483 341 186 168
149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3580 3648 4009 4330 5642 5659 5812
Size: 1255 2000 68 361 321 1312 17 153
Cuts at: 5812 5997 6442 325
Size: 185 445 1498
Fragments arranged by size:
2000 1498 1312 1255 445 361 321 185
153 68 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2340 2631 2646
Size: 274 80 33 51 1443 363 291 15
Cuts at: 2646 2652 4665 5861 7573 96
Size: 6 2013 1196 1712 138
Fragments arranged by size:
2013 1712 1443 1196 363 291 274 138
80 51 33 15 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2397 2429 4018 4050 7575 7607 2397
Size: 32 1589 32 3525 32 2405
Fragments arranged by size:
3525 2405 1589 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3988 1316
Size: 2672 4943
XbaI T'CTAG_A
Cuts at: 4951 4951
Size: 7615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2262 4335 2262
Size: 2073 5542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7615
XmaI C'CCGG_G
Cuts at: 1870 2280 3792 4731 4940 1870
Size: 410 1512 939 209 4545
Fragments arranged by size:
4545 1512 939 410 209
XmnI GAAnn'nnTTC
Cuts at: 5744 5744
Size: 7615
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2502 5423 276
Size: 53 83 186 936 968 2921 2468
Fragments arranged by size:
2921 2468 968 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI