Restriction map for
pFN21AA0847
(Circular) (Six-base) MAPSORT of: pFN21AA0847.seq Check: 6938 from: 1 to: 8437
pFN21AA0847 8437 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2535 6247 278
Size: 53 83 186 936 999 3712 2468
Fragments arranged by size:
3712 2468 999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2371 5780 1937
Size: 434 3409 4594
Fragments arranged by size:
4594 3409 434
Acc65I G'GTAC_C
Cuts at: 5758 5758
Size: 8437
AclI AA'CG_TT
Cuts at: 1563 6564 6937 1563
Size: 5001 373 3063
Fragments arranged by size:
5001 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 3231 3671 3774 3876 4029 4073 4184
Size: 539 1060 440 103 102 153 44 111
Cuts at: 4184 4356 4542 5133 6500 7884 8152 1632
Size: 172 186 591 1367 1384 268 1917
Fragments arranged by size:
1917 1384 1367 1060 591 539 440 268
186 172 153 111 103 102 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8240
AflIII A'CryG_T
Cuts at: 1203 2468 2646 4224 4588 7336 8313 1203
Size: 1265 178 1578 364 2748 977 1327
Fragments arranged by size:
2748 1578 1327 1265 977 364 178
AgeI A'CCGG_T
Cuts at: 7268 7268
Size: 8437
AhdI GACnn_n'nnGTC
Cuts at: 2632 7166 2632
Size: 4534 3903
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8437
AlwNI CAG_nnn'CTG
Cuts at: 3392 4892 6180 7752 3392
Size: 1500 1288 1572 4077
Fragments arranged by size:
4077 1572 1500 1288
ApaLI G'TGCA_C
Cuts at: 6494 7650 6494
Size: 1156 7281
ApoI r'AATT_y
Cuts at: 1493 2106 2765 3192 4310 4563 5160 5343 5746
Size: 613 659 427 1118 253 597 183 403
Cuts at: 5746 5972 6124 8233 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 659 613 597 427 403
253 226 183 152
AseI AT'TA_AT
Cuts at: 160 1032 6991 160
Size: 872 5959 1606
Fragments arranged by size:
5959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8437
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2544 5762 1870
Size: 73 388 213 3218 4545
Fragments arranged by size:
4545 3218 388 213 73
AvrII C'CTAG_G
Cuts at: 5296 5296
Size: 8437
BamHI G'GATC_C
Cuts at: 5657 5767 6134 5657
Size: 110 367 7960
Fragments arranged by size:
7960 367 110
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4504 4781 5758 7213 618
Size: 325 217 627 2717 277 977 1455 1842
Fragments arranged by size:
2717 1842 1455 977 627 325 277 217
BanII G_rGCy'C
Cuts at: 1391 3629 4104 5756 1391
Size: 2238 475 1652 4072
Fragments arranged by size:
4072 2238 1652 475
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2623 2906 3236 4671 928
Size: 971 724 283 330 1435 4694
Fragments arranged by size:
4694 1435 971 724 330 283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6628 6662 1903
Size: 34 4691 34 3678
Fragments arranged by size:
4691 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6329 7539 6329
Size: 1210 7227
BclI T'GATC_A
Cuts at: 1548 3823 4285 1548
Size: 2275 462 5700
Fragments arranged by size:
5700 2275 462
BfrBI ATG'CAT
Cuts at: 2582 2582
Size: 8437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7048 136
Size: 107 122 71 631 605 5376 1525
Fragments arranged by size:
5376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2311 4084 4407 8432 2311
Size: 1773 323 4025 2316
Fragments arranged by size:
4025 2316 1773 323
BlpI GC'TnA_GC
Cuts at: 3059 5854 3059
Size: 2795 5642
Bme1580I G_kGCm'C
Cuts at: 1790 4786 6498 7654 1790
Size: 2996 1712 1156 2573
Fragments arranged by size:
2996 2573 1712 1156
BmgBI CAC'GTC
Cuts at: 1318 5493 1318
Size: 4175 4262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2902 5137 5451
Size: 416 53 83 399 24 1485 2235 314
Cuts at: 5451 6168 7126 442
Size: 717 958 1753
Fragments arranged by size:
2235 1753 1485 958 717 416 399 314
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4429 4879 5397 4429
Size: 450 518 7469
Fragments arranged by size:
7469 518 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2701 2745 3030 3395 3437 3782 3791 3905
Size: 1462 44 285 365 42 345 9 114
Cuts at: 3905 3948 4869 5283 5587 7097 1239
Size: 43 921 414 304 1510 2579
Fragments arranged by size:
2579 1510 1462 921 414 365 345 304
285 114 44 43 42 9
Bpu10I CC'TnA_GC
Cuts at: 3576 3623 3576
Size: 47 8390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3425 3768 4242 4975 6562 7427 7725 3425
Size: 343 474 733 1587 865 298 4137
Fragments arranged by size:
4137 1587 865 733 474 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3401 4680 7100 882
Size: 617 1902 1279 2420 2219
Fragments arranged by size:
2420 2219 1902 1279 617
BsaAI yAC'GTr
Cuts at: 493 1204 6228 493
Size: 711 5024 2702
Fragments arranged by size:
5024 2702 711
BsaBI GATnn'nnATC
Cuts at: 8431 8431
Size: 8437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2532 3956 6244 6626
Size: 53 83 186 936 999 1424 2288 382
Cuts at: 6626 7261 275
Size: 635 1451
Fragments arranged by size:
2288 1451 1424 999 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6870 7268 7542 7689 8100 6870
Size: 398 274 147 411 7207
Fragments arranged by size:
7207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4416 4446 5312 5342 5443
Size: 30 165 30 3013 30 866 30 101
Cuts at: 5443 5473 1178
Size: 30 4142
Fragments arranged by size:
4142 3013 866 165 101 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 3230 3761 4402 4898 5030 5066 5394
Size: 845 1199 531 641 496 132 36 328
Cuts at: 5394 5457 5554 5590 1186
Size: 63 97 36 4033
Fragments arranged by size:
4033 1199 845 641 531 496 328 132
97 63 36 36
BseYI C'CCAG_C
Cuts at: 2365 5579 5636 7640 8136 2365
Size: 3214 57 2004 496 2666
Fragments arranged by size:
3214 2666 2004 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3462 1163
Size: 573 1726 6138
Fragments arranged by size:
6138 1726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5887 6648 7676 1540
Size: 275 186 3886 761 1028 2301
Fragments arranged by size:
3886 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3629 5064 5378 6498 6583 7654 3629
Size: 1435 314 1120 85 1071 4412
Fragments arranged by size:
4412 1435 1120 1071 314 85
BsmI GAATG_Cn'
Cuts at: 2397 5948 6041 2397
Size: 3551 93 4793
Fragments arranged by size:
4793 3551 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5220 5220
Size: 8437
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3629 4104 4786 5064 5378 5756 6498
Size: 399 1839 475 682 278 314 378 742
Cuts at: 6498 6583 7654 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1839 1071 742 682 475 399 378
314 278 85
BspEI T'CCGG_A
Cuts at: 8100 8100
Size: 8437
BspHI T'CATG_A
Cuts at: 4219 4656 6326 8056 4219
Size: 437 1670 1730 4600
Fragments arranged by size:
4600 1730 1670 437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2546 3419 5792 844
Size: 1702 873 2373 3489
Fragments arranged by size:
3489 2373 1702 873
BsrBI CCG'CTC
Cuts at: 1394 1823 3285 6324 1394
Size: 429 1462 3039 3507
Fragments arranged by size:
3507 3039 1462 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3607 5626 6932 7106 65
Size: 1183 2359 2019 1306 174 1396
Fragments arranged by size:
2359 2019 1396 1306 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7081 7268 1700
Size: 110 5271 187 2869
Fragments arranged by size:
5271 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8437
BssSI C'ACGA_G
Cuts at: 5254 6497 7509 5254
Size: 1243 1012 6182
Fragments arranged by size:
6182 1243 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3866 794
Size: 3072 5365
BstBI TT'CG_AA
Cuts at: 7241 7241
Size: 8437
BstEII G'GTnAC_C
Cuts at: 1184 3267 1184
Size: 2083 6354
BstXI CCAn_nnnn'nTGG
Cuts at: 3239 5178 3239
Size: 1939 6498
BstYI r'GATC_y
Cuts at: 1972 2311 3113 4084 4407 5357 5399 5591 5657
Size: 339 802 971 323 950 42 192 66
Cuts at: 5657 5767 6134 6519 6536 7988 8074 8086 8432
Size: 110 367 385 17 1452 86 12 346
Cuts at: 8432 1972
Size: 1977
Fragments arranged by size:
1977 1452 971 950 802 385 367 346
339 323 192 110 86 66 42 17
12
Bsu36I CC'TnA_GG
Cuts at: 3844 3844
Size: 8437
BtgI C'CryG_G
Cuts at: 513 1065 3226 4095 513
Size: 552 2161 869 4855
Fragments arranged by size:
4855 2161 869 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3078 487
Size: 683 56 1852 5846
Fragments arranged by size:
5846 1852 683 56
BtsI GCAGTG_nn'
Cuts at: 5953 6747 6767 5953
Size: 794 20 7623
Fragments arranged by size:
7623 794 20
ClaI AT'CG_AT
Cuts at: 1554 2266 1554
Size: 712 7725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2506 2541 529
Size: 35 1942 35 6425
Fragments arranged by size:
6425 1942 35 35
DraI TTT'AAA
Cuts at: 3831 5741 3831
Size: 1910 6527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3129 7444 809
Size: 507 1813 4315 1802
Fragments arranged by size:
4315 1813 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3229 5231 5884
Size: 54 1425 50 275 18 1399 2002 653
Cuts at: 5884 6773 8402 8
Size: 889 1629 43
Fragments arranged by size:
2002 1629 1425 1399 889 653 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5884 1537
Size: 275 4072 4090
Fragments arranged by size:
4090 4072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2797 3150 3207 4332 5702 6367 1353
Size: 1444 353 57 1125 1370 665 3423
Fragments arranged by size:
3423 1444 1370 1125 665 353 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7024 7398 7544 387
Size: 1064 138 5435 374 146 1280
Fragments arranged by size:
5435 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3627 3627
Size: 8437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2701 2745 3030 3231 3395 3437
Size: 393 539 530 44 285 201 164 42
Cuts at: 3437 3671 3774 3782 3791 3876 3905 3948 4029
Size: 234 103 8 9 85 29 43 81
Cuts at: 4029 4073 4184 4356 4542 4869 5133 5283 5587
Size: 44 111 172 186 327 264 150 304
Cuts at: 5587 6500 7097 7884 8152 1239
Size: 913 597 787 268 1524
Fragments arranged by size:
1524 913 787 597 539 530 393 327
304 285 268 264 234 201 186 172
164 150 111 103 85 81 44 44
43 42 29 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 4173 5602 5605 4173
Size: 1429 3 7005
Fragments arranged by size:
7005 1429 3
EcoO109I rG'GnC_Cy
Cuts at: 3682 3806 3875 3989 5610 3682
Size: 124 69 114 1621 6509
Fragments arranged by size:
6509 1621 124 114 69
EcoRI G'AATT_C
Cuts at: 2765 5746 6124 2765
Size: 2981 378 5078
Fragments arranged by size:
5078 2981 378
EcoRV GAT'ATC
Cuts at: 2032 4638 2032
Size: 2606 5831
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3554 4010 3554
Size: 456 7981
FspI TGC'GCA
Cuts at: 6943 6943
Size: 8437
HaeII r_GCGC'y
Cuts at: 1678 7584 1678
Size: 5906 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 2618 2650 2774
Size: 32 135 32 676 32 338 32 124
Cuts at: 2774 2806 4399 4431 4713 4745 5277 5309 5312
Size: 32 1593 32 282 32 532 32 3
Cuts at: 5312 5344 7078 7110 7152 7184 1373
Size: 32 1734 32 42 32 2626
Fragments arranged by size:
2626 1734 1593 676 532 338 282 135
124 42 32 32 32 32 32 32
32 32 32 32 32 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3619 4041 5185 5781 6027 669
Size: 988 281 1681 422 1144 596 246 3079
Fragments arranged by size:
3079 1681 1144 988 596 422 281 246
HindIII A'AGCT_T
Cuts at: 748 3185 4051 4538 4943 748
Size: 2437 866 487 405 4242
Fragments arranged by size:
4242 2437 866 487 405
HpaI GTT'AAC
Cuts at: 3619 6027 3619
Size: 2408 6029
KpnI G_GTAC'C
Cuts at: 5762 5762
Size: 8437
MfeI C'AATT_G
Cuts at: 2979 6036 2979
Size: 3057 5380
MluI A'CGCG_T
Cuts at: 8313 8313
Size: 8437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2023 2279 4531 5337 7551 7735 1305
Size: 718 256 2252 806 2214 184 2007
Fragments arranged by size:
2252 2214 2007 806 718 256 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3231 10
Size: 54 1425 343 1399 5216
Fragments arranged by size:
5216 1425 1399 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2651 3525 6395 6754 6913 518
Size: 648 1485 874 2870 359 159 2042
Fragments arranged by size:
2870 2042 1485 874 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2674 5111 5853 6530 7678 7923 1606
Size: 1068 2437 742 677 1148 245 2120
Fragments arranged by size:
2437 2120 1148 1068 742 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3226 4095 513
Size: 552 2161 869 4855
Fragments arranged by size:
4855 2161 869 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8437
NotI GC'GGCC_GC
Cuts at: 5884 5884
Size: 8437
NsiI A_TGCA'T
Cuts at: 2584 2584
Size: 8437
NspI r_CATG'y
Cuts at: 1132 1229 2128 2472 2488 2650 4228 4592 5795
Size: 97 899 344 16 162 1578 364 1203
Cuts at: 5795 7340 1132
Size: 1545 2229
Fragments arranged by size:
2229 1578 1545 1203 899 364 344 162
97 16
PasI CC'CwG_GG
Cuts at: 1348 4325 1348
Size: 2977 5460
PciI A'CATG_T
Cuts at: 2468 2646 4224 4588 7336 2468
Size: 178 1578 364 2748 3569
Fragments arranged by size:
3569 2748 1578 364 178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3263 3657 1108
Size: 2155 394 5888
Fragments arranged by size:
5888 2155 394
PfoI T'CCnGG_A
Cuts at: 3768 5395 5603 3768
Size: 1627 208 6602
Fragments arranged by size:
6602 1627 208
PmeI GTTT'AAAC
Cuts at: 5741 5741
Size: 8437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2556 2588 6506 6538 8019 8051 2556
Size: 32 3918 32 1481 32 2942
Fragments arranged by size:
3918 2942 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3682 3806 3875 3989 5610 3682
Size: 124 69 114 1621 6509
Fragments arranged by size:
6509 1621 124 114 69
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8437
PsiI TTA'TAA
Cuts at: 6007 6007
Size: 8437
PspXI vC'TCGA_Gb
Cuts at: 2544 2544
Size: 8437
PstI C_TGCA'G
Cuts at: 830 3871 5789 830
Size: 3041 1918 3478
Fragments arranged by size:
3478 3041 1918
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8437
PvuII CAG'CTG
Cuts at: 2674 5111 2674
Size: 2437 6000
SacI G_AGCT'C
Cuts at: 3629 3629
Size: 8437
SalI G'TCGA_C
Cuts at: 1936 5779 1936
Size: 3843 4594
SbfI CC_TGCA'GG
Cuts at: 5789 5789
Size: 8437
ScaI AGT'ACT
Cuts at: 3759 6685 8397 3759
Size: 2926 1712 3799
Fragments arranged by size:
3799 2926 1712
SfcI C'TryA_G
Cuts at: 826 1045 3711 3867 5785 6920 7601 7792 826
Size: 219 2666 156 1918 1135 681 191 1471
Fragments arranged by size:
2666 1918 1471 1135 681 219 191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8437
SmaI CCC'GGG
Cuts at: 1872 5764 1872
Size: 3892 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2544 3404 3783 4257 4990 6541
Size: 197 926 601 860 379 474 733 1551
Cuts at: 6541 7442 7704 820
Size: 901 262 1553
Fragments arranged by size:
1553 1551 926 901 860 733 601 474
379 262 197
SnaBI TAC'GTA
Cuts at: 493 6228 493
Size: 5735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8437
SphI G_CATG'C
Cuts at: 1132 5795 1132
Size: 4663 3774
SspI AAT'ATT
Cuts at: 5 52 6361 5
Size: 47 6309 2081
Fragments arranged by size:
6309 2081 47
StuI AGG'CCT
Cuts at: 1517 5260 1517
Size: 3743 4694
StyI C'CwwG_G
Cuts at: 513 1065 2118 3226 3262 3809 4095 4124 4978
Size: 552 1053 1108 36 547 286 29 854
Cuts at: 4978 5165 5296 5701 5876 513
Size: 187 131 405 175 3074
Fragments arranged by size:
3074 1108 1053 854 552 547 405 286
187 175 131 36 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3649 5146 5266 5440 6464 6481 6634
Size: 1255 2069 1497 120 174 1024 17 153
Cuts at: 6634 6819 7264 325
Size: 185 445 1498
Fragments arranged by size:
2069 1498 1497 1255 1024 445 185 174
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2465 3757 6683
Size: 274 80 33 51 1443 488 1292 2926
Cuts at: 6683 8395 96
Size: 1712 138
Fragments arranged by size:
2926 1712 1443 1292 488 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 5443 5475 8397 8429 2037
Size: 32 3374 32 2922 32 2045
Fragments arranged by size:
3374 2922 2045 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4937 1316
Size: 3621 4816
XbaI T'CTAG_A
Cuts at: 2779 5147 5773 2779
Size: 2368 626 5443
Fragments arranged by size:
5443 2368 626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 3967 2362
Size: 1605 6832
XhoI C'TCGA_G
Cuts at: 1943 2544 1943
Size: 601 7836
XmaI C'CCGG_G
Cuts at: 1870 5762 1870
Size: 3892 4545
XmnI GAAnn'nnTTC
Cuts at: 2397 3456 3537 3736 3759 4752 6566 2397
Size: 1059 81 199 23 993 1814 4268
Fragments arranged by size:
4268 1814 1059 993 199 81 23
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2533 6245 276
Size: 53 83 186 936 999 3712 2468
Fragments arranged by size:
3712 2468 999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BsiWI BstZ17I DraIII FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI