Restriction map for
pFN21AA0840
(Circular) (Six-base) MAPSORT of: pFN21AA0840.seq Check: 5710 from: 1 to: 6178
pFN21AA0840 6178 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3521 1937
Size: 1584 4594
Acc65I G'GTAC_C
Cuts at: 3063 3499 3063
Size: 436 5742
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4241 5625 5893 1632
Size: 2609 1384 268 1917
Fragments arranged by size:
2609 1917 1384 268
AfeI AGC'GCT
Cuts at: 3443 3443
Size: 6178
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 2553 5077 6054 1203
Size: 1350 2524 977 1327
Fragments arranged by size:
2524 1350 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 2657 4907 2657
Size: 2250 3928
AleI CACnn'nnGTG
Cuts at: 1166 3098 1166
Size: 1932 4246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3152 1184
Size: 1968 4210
AlwNI CAG_nnn'CTG
Cuts at: 2535 2589 3003 3379 3921 5493 2535
Size: 54 414 376 542 1572 3220
Fragments arranged by size:
3220 1572 542 414 376 54
ApaLI G'TGCA_C
Cuts at: 2267 4235 5391 2267
Size: 1968 1156 3054
Fragments arranged by size:
3054 1968 1156
ApoI r'AATT_y
Cuts at: 1493 3487 3713 3865 5974 1493
Size: 1994 226 152 2109 1697
Fragments arranged by size:
2109 1994 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2775 3503 1870
Size: 73 110 722 728 4545
Fragments arranged by size:
4545 728 722 110 73
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3063 3499 4954 618
Size: 325 217 627 1276 436 1455 1842
Fragments arranged by size:
1842 1455 1276 627 436 325 217
BanII G_rGCy'C
Cuts at: 1391 2222 3497 1391
Size: 831 1275 4072
Fragments arranged by size:
4072 1275 831
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 928
Size: 971 237 4970
Fragments arranged by size:
4970 971 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3259 4070 5280 3259
Size: 811 1210 4157
Fragments arranged by size:
4157 1210 811
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6178
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2484 4789 136
Size: 107 122 71 631 605 812 2305 1525
Fragments arranged by size:
2305 1525 812 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2376 6173 2376
Size: 3797 2381
BlpI GC'TnA_GC
Cuts at: 2990 3302 3595 2990
Size: 312 293 5573
Fragments arranged by size:
5573 312 293
Bme1580I G_kGCm'C
Cuts at: 1790 2271 4239 5395 1790
Size: 481 1968 1156 2573
Fragments arranged by size:
2573 1968 1156 481
BmgBI CAC'GTC
Cuts at: 1318 2071 3100 1318
Size: 753 1029 4396
Fragments arranged by size:
4396 1029 753
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 3909 4867
Size: 416 53 83 399 24 1206 1286 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1753 1286 1206 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2302 2485 2744 2879 3068 3284 3356 4838
Size: 1063 183 259 135 189 216 72 1482
Cuts at: 4838 1239
Size: 2579
Fragments arranged by size:
2579 1482 1063 259 216 189 183 135
72
Bpu10I CC'TnA_GC
Cuts at: 2141 3068 2141
Size: 927 5251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 3278 3293 4303 5168 5466 2498
Size: 780 15 1010 865 298 3210
Fragments arranged by size:
3210 1010 865 780 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2483 3406 4841 882
Size: 617 984 923 1435 2219
Fragments arranged by size:
2219 1435 984 923 617
BsaAI yAC'GTr
Cuts at: 493 1204 3115 3969 493
Size: 711 1911 854 2702
Fragments arranged by size:
2702 1911 854 711
BsaBI GATnn'nnATC
Cuts at: 2822 6172 2822
Size: 3350 2828
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2178 2208 2663 2693 2960
Size: 30 165 30 775 30 455 30 267
Cuts at: 2960 2990 1178
Size: 30 4366
Fragments arranged by size:
4366 775 455 267 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 1186
Size: 1035 5143
BseYI C'CCAG_C
Cuts at: 2165 2170 5381 5877 2165
Size: 5 3211 496 2466
Fragments arranged by size:
3211 2466 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 2858 2878 3190 1163
Size: 573 657 465 20 312 4151
Fragments arranged by size:
4151 657 573 465 312 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3628 4389 5417 1540
Size: 275 186 1087 540 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 540 275 186
BsiHKAI G_wGCw'C
Cuts at: 2162 2271 2730 3459 4239 4324 5395 2162
Size: 109 459 729 780 85 1071 2945
Fragments arranged by size:
2945 1071 780 729 459 109 85
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
BsmBI CGTCTCn'nnnn_
Cuts at: 2246 2246
Size: 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2222 2271 2730 3459 3497 4239
Size: 399 372 60 49 459 729 38 742
Cuts at: 4239 4324 5395 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 459 399 372 85
60 49 38
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 4067 5797 4067
Size: 1730 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 2925 3533 844
Size: 1924 157 608 3489
Fragments arranged by size:
3489 1924 608 157
BsrBI CCG'CTC
Cuts at: 1394 1823 2302 4065 1394
Size: 429 479 1763 3507
Fragments arranged by size:
3507 1763 479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2349 3329 4822 5009 1700
Size: 110 539 980 1493 187 2869
Fragments arranged by size:
2869 1493 980 539 187 110
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 3658
BssHII G'CGCG_C
Cuts at: 1925 2415 1925
Size: 490 5688
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2535 3444 794
Size: 1741 909 3528
Fragments arranged by size:
3528 1741 909
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 3091 1184
Size: 1907 4271
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 2500
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2376 3508 3875 4260 4277 5729 5815 5827
Size: 404 1132 367 385 17 1452 86 12
Cuts at: 5827 6173 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1132 404 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 2367 2456 3178 3405 513
Size: 552 939 258 105 89 722 227 3286
Fragments arranged by size:
3286 939 722 552 258 227 105 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2608 2866 3058 487
Size: 683 56 1382 258 192 3607
Fragments arranged by size:
3607 1382 683 258 192 56
BtsI GCAGTG_nn'
Cuts at: 2041 2890 3079 3433 3694 4488 4508 2041
Size: 849 189 354 261 794 20 3711
Fragments arranged by size:
3711 849 794 354 261 189 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DraIII CAC_nnn'GTG
Cuts at: 3184 3184
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3398 5185 809
Size: 507 2082 1787 1802
Fragments arranged by size:
2082 1802 1787 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 3085 3117
Size: 54 1425 50 275 18 429 826 32
Cuts at: 3117 3331 3351 3625 4514 6143 8
Size: 214 20 274 889 1629 43
Fragments arranged by size:
1629 1425 889 826 429 275 274 214
54 50 43 32 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 3625 1537
Size: 275 1273 540 4090
Fragments arranged by size:
4090 1273 540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4108 1353
Size: 2755 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2465 2921 4765 5139 5285 387
Size: 1064 138 876 456 1844 374 146 1280
Fragments arranged by size:
1844 1280 1064 876 456 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2302 2485 2744 2879 3068 3284 3356
Size: 393 670 183 259 135 189 216 72
Cuts at: 3356 4241 4838 5625 5893 1239
Size: 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 670 597 393 268 259
216 189 183 135 72
EcoO109I rG'GnC_Cy
Cuts at: 2452 3412 2452
Size: 960 5218
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
EcoRV GAT'ATC
Cuts at: 3229 3229
Size: 6178
FspI TGC'GCA
Cuts at: 2150 4684 2150
Size: 2534 3644
FspAI rTGC'GCAy
Cuts at: 2150 2150
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 3445 5325 1678
Size: 1767 1880 2531
Fragments arranged by size:
2531 1880 1767
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2643 2675 2690 2722 4819
Size: 32 135 32 1071 32 15 32 2097
Cuts at: 4819 4851 4893 4925 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2097 1071 135 42 32 32 32
32 32 32 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3522 3768 669
Size: 988 281 1584 246 3079
Fragments arranged by size:
3079 1584 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6178
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 3067 3503 3067
Size: 436 5742
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 2706 3276 5292 5476 1305
Size: 855 546 570 2016 184 2007
Fragments arranged by size:
2016 2007 855 570 546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 3119 10
Size: 54 1425 343 429 858 3069
Fragments arranged by size:
3069 1425 858 429 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2498 2733 3098 4136 4495 4654 518
Size: 648 1332 235 365 1038 359 159 2042
Fragments arranged by size:
2042 1332 1038 648 365 359 235 159
MspA1I CmG'CkG
Cuts at: 1606 2404 2412 2433 2458 2925 3594 4271 5419
Size: 798 8 21 25 467 669 677 1148
Cuts at: 5419 5664 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 798 677 669 467 245 25
21 8
NcoI C'CATG_G
Cuts at: 513 1065 2004 2262 2367 513
Size: 552 939 258 105 4324
Fragments arranged by size:
4324 939 552 258 105
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2149 3536 5081 1132
Size: 97 920 1387 1545 2229
Fragments arranged by size:
2229 1545 1387 920 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2368 3184 1108
Size: 1260 816 4102
Fragments arranged by size:
4102 1260 816
PfoI T'CCnGG_A
Cuts at: 2355 3387 2355
Size: 1032 5146
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2452
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PspXI vC'TCGA_Gb
Cuts at: 2053 2053
Size: 6178
PstI C_TGCA'G
Cuts at: 830 2594 3131 3530 830
Size: 1764 537 399 3478
Fragments arranged by size:
3478 1764 537 399
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
PvuII CAG'CTG
Cuts at: 2404 2404
Size: 6178
SacII CC_GC'GG
Cuts at: 2459 2459
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3520 1936
Size: 1584 4594
SanDI GG'GwC_CC
Cuts at: 2452 2452
Size: 6178
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 3125 4426 6138 3125
Size: 1301 1712 3165
Fragments arranged by size:
3165 1712 1301
SexAI A'CCwGG_T
Cuts at: 2958 2958
Size: 6178
SfcI C'TryA_G
Cuts at: 826 1045 2590 3127 3526 4661 5342 5533 826
Size: 219 1545 537 399 1135 681 191 1471
Fragments arranged by size:
1545 1471 1135 681 537 399 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 2777 3505 1872
Size: 905 728 4545
Fragments arranged by size:
4545 905 728
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2053 2513 3293 3308 4282 5183
Size: 197 926 110 460 780 15 974 901
Cuts at: 5183 5445 820
Size: 262 1553
Fragments arranged by size:
1553 974 926 901 780 460 262 197
110 15
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 2149 3536 1132
Size: 1017 1387 3774
Fragments arranged by size:
3774 1387 1017
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 2783 2871 2949 1517
Size: 1266 88 78 4746
Fragments arranged by size:
4746 1266 88 78
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 2367 3617 513
Size: 552 939 258 105 1250 3074
Fragments arranged by size:
3074 1250 939 552 258 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2622 2921 2954 4205 4222 4375 4560
Size: 1255 1042 299 33 1251 17 153 185
Cuts at: 4560 5005 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1251 1042 445 299 185 153
33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2081 2616 3123
Size: 274 80 33 51 1443 104 535 507
Cuts at: 3123 4424 6136 96
Size: 1301 1712 138
Fragments arranged by size:
1712 1443 1301 535 507 274 138 104
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 6138 6170 2178
Size: 32 3928 32 2186
Fragments arranged by size:
3928 2186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2830 2830
Size: 6178
XhoI C'TCGA_G
Cuts at: 1943 2053 1943
Size: 110 6068
XmaI C'CCGG_G
Cuts at: 1870 2775 3503 1870
Size: 905 728 4545
Fragments arranged by size:
4545 905 728
XmnI GAAnn'nnTTC
Cuts at: 3284 4307 3284
Size: 1023 5155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI
FseI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PsrI RsrII SacI SapI SfiI SfoI
SrfI SwaI