Restriction map for
pFN21AA0837
(Circular) (Six-base) MAPSORT of: pFN21AA0837.seq Check: 7472 from: 1 to: 6871
pFN21AA0837 6871 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4681 278
Size: 53 83 186 936 3145 2468
Fragments arranged by size:
3145 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4214 1937
Size: 2277 4594
Acc65I G'GTAC_C
Cuts at: 4192 4192
Size: 6871
AclI AA'CG_TT
Cuts at: 1563 4998 5371 1563
Size: 3435 373 3063
Fragments arranged by size:
3435 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3196 3915 3989 4934 6318 6586 1632
Size: 1564 719 74 945 1384 268 1917
Fragments arranged by size:
1917 1564 1384 945 719 268 74
AfeI AGC'GCT
Cuts at: 3239 3239
Size: 6871
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6674
AflIII A'CryG_T
Cuts at: 1203 3051 4062 5770 6747 1203
Size: 1848 1011 1708 977 1327
Fragments arranged by size:
1848 1708 1327 1011 977
AgeI A'CCGG_T
Cuts at: 5702 5702
Size: 6871
AhdI GACnn_n'nnGTC
Cuts at: 5600 5600
Size: 6871
AleI CACnn'nnGTG
Cuts at: 1166 2168 1166
Size: 1002 5869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3899 3967 1184
Size: 2715 68 4088
Fragments arranged by size:
4088 2715 68
AlwNI CAG_nnn'CTG
Cuts at: 2440 4614 6186 2440
Size: 2174 1572 3125
Fragments arranged by size:
3125 2174 1572
ApaI G_GGCC'C
Cuts at: 2400 2627 3916 2400
Size: 227 1289 5355
Fragments arranged by size:
5355 1289 227
ApaLI G'TGCA_C
Cuts at: 3466 4928 6084 3466
Size: 1462 1156 4253
Fragments arranged by size:
4253 1462 1156
ApoI r'AATT_y
Cuts at: 1493 2471 4180 4406 4558 6667 1493
Size: 978 1709 226 152 2109 1697
Fragments arranged by size:
2109 1709 1697 978 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5425 160
Size: 872 4393 1606
Fragments arranged by size:
4393 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6871
AvaI C'yCGr_G
Cuts at: 1870 1943 3788 4196 1870
Size: 73 1845 408 4545
Fragments arranged by size:
4545 1845 408 73
BamHI G'GATC_C
Cuts at: 4201 4568 4201
Size: 367 6504
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2180 3432 3523 4192 5647
Size: 325 217 627 393 1252 91 669 1455
Cuts at: 5647 618
Size: 1842
Fragments arranged by size:
1842 1455 1252 669 627 393 325 217
91
BanII G_rGCy'C
Cuts at: 1391 2036 2400 2627 3916 4190 1391
Size: 645 364 227 1289 274 4072
Fragments arranged by size:
4072 1289 645 364 274 227
BbeI G_GCGC'C
Cuts at: 3527 3527
Size: 6871
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5900
BbvCI CC'TCA_GC
Cuts at: 2316 2316
Size: 6871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5062 5096 1903
Size: 34 50 34 3041 34 3678
Fragments arranged by size:
3678 3041 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2097 4763 5973 2097
Size: 2666 1210 2995
Fragments arranged by size:
2995 2666 1210
BclI T'GATC_A
Cuts at: 1548 2518 2748 1548
Size: 970 230 5671
Fragments arranged by size:
5671 970 230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2465 5482 136
Size: 107 122 71 631 605 793 3017 1525
Fragments arranged by size:
3017 1525 793 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3207 3946 6866 3207
Size: 739 2920 3212
Fragments arranged by size:
3212 2920 739
BlpI GC'TnA_GC
Cuts at: 2378 4288 2378
Size: 1910 4961
Bme1580I G_kGCm'C
Cuts at: 1790 2185 2290 2400 2627 2871 3470 3516 3916
Size: 395 105 110 227 244 599 46 400
Cuts at: 3916 4932 6088 1790
Size: 1016 1156 2573
Fragments arranged by size:
2573 1156 1016 599 400 395 244 227
110 105 46
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6871
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3586 4602 5560
Size: 416 53 83 399 24 2169 1016 958
Cuts at: 5560 442
Size: 1753
Fragments arranged by size:
2169 1753 1016 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6871
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4174 4174
Size: 6871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2477 2718 3269 3399 3714 5531 1239
Size: 854 384 241 551 130 315 1817 2579
Fragments arranged by size:
2579 1817 854 551 384 315 241 130
Bpu10I CC'TnA_GC
Cuts at: 2118 2316 2421 3768 4116 2118
Size: 198 105 1347 348 4873
Fragments arranged by size:
4873 1347 348 198 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2726 4996 5861 6159 2726
Size: 2270 865 298 3438
Fragments arranged by size:
3438 2270 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4105 5534 882
Size: 617 2606 1429 2219
Fragments arranged by size:
2606 2219 1429 617
BsaAI yAC'GTr
Cuts at: 493 1204 3517 4662 493
Size: 711 2313 1145 2702
Fragments arranged by size:
2702 2313 1145 711
BsaBI GATnn'nnATC
Cuts at: 6865 6865
Size: 6871
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3524 4678 5060 5695
Size: 53 83 186 936 1991 1154 382 635
Cuts at: 5695 275
Size: 1451
Fragments arranged by size:
1991 1451 1154 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2483 3192 3282 3813 5304 5702 5976 6123 6534
Size: 709 90 531 1491 398 274 147 411
Cuts at: 6534 2483
Size: 2820
Fragments arranged by size:
2820 1491 709 531 411 398 274 147
90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3084 3114 3765 3795 4148
Size: 30 165 30 1681 30 651 30 353
Cuts at: 4148 4178 1178
Size: 30 3871
Fragments arranged by size:
3871 1681 651 353 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6871
BseYI C'CCAG_C
Cuts at: 3388 6074 6570 3388
Size: 2686 496 3689
Fragments arranged by size:
3689 2686 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3937 1163
Size: 573 2201 4097
Fragments arranged by size:
4097 2201 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4321 5082 6110 1540
Size: 275 186 2320 761 1028 2301
Fragments arranged by size:
2320 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3027 3470 4932 5017 6088 3027
Size: 443 1462 85 1071 3810
Fragments arranged by size:
3810 1462 1071 443 85
BsmI GAATG_Cn'
Cuts at: 4382 4475 4382
Size: 93 6778
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2185 2290 2400 2627 2871 3027
Size: 399 246 149 105 110 227 244 156
Cuts at: 3027 3470 3516 3916 4190 4932 5017 6088 1391
Size: 443 46 400 274 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 400 399 274 246
244 227 156 149 110 105 85 46
BspEI T'CCGG_A
Cuts at: 2483 3282 3813 6534 2483
Size: 799 531 2721 2820
Fragments arranged by size:
2820 2721 799 531
BspHI T'CATG_A
Cuts at: 4760 6490 4760
Size: 1730 5141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4024 4226 844
Size: 3180 202 3489
Fragments arranged by size:
3489 3180 202
BsrBI CCG'CTC
Cuts at: 1394 1823 2608 4758 1394
Size: 429 785 2150 3507
Fragments arranged by size:
3507 2150 785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5366 5540 65
Size: 1183 4118 174 1396
Fragments arranged by size:
4118 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 3715 5515 5702 1700
Size: 110 407 1498 1800 187 2869
Fragments arranged by size:
2869 1800 1498 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6871
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6871
BssSI C'ACGA_G
Cuts at: 2021 2168 4931 5943 2021
Size: 147 2763 1012 2949
Fragments arranged by size:
2949 2763 1012 147
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6871
BstBI TT'CG_AA
Cuts at: 2769 5675 2769
Size: 2906 3965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6871
BstXI CCAn_nnnn'nTGG
Cuts at: 2599 2599
Size: 6871
BstYI r'GATC_y
Cuts at: 1972 2094 3207 3643 3946 3964 4201 4568 4953
Size: 122 1113 436 303 18 237 367 385
Cuts at: 4953 4970 6422 6508 6520 6866 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1113 436 385 367 346 303
237 122 86 18 17 12
Bsu36I CC'TnA_GG
Cuts at: 2099 3508 2099
Size: 1409 5462
BtgI C'CryG_G
Cuts at: 513 1065 2371 2592 2829 2947 3088 3844 4167
Size: 552 1306 221 237 118 141 756 323
Cuts at: 4167 513
Size: 3217
Fragments arranged by size:
3217 1306 756 552 323 237 221 141
118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2951 3690 487
Size: 683 56 1725 739 3668
Fragments arranged by size:
3668 1725 739 683 56
BtsI GCAGTG_nn'
Cuts at: 4387 5181 5201 4387
Size: 794 20 6057
Fragments arranged by size:
6057 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6871
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6836
DraI TTT'AAA
Cuts at: 3760 4175 3760
Size: 415 6456
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5878 809
Size: 507 4562 1802
Fragments arranged by size:
4562 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2219 2457
Size: 54 1425 50 275 18 327 62 238
Cuts at: 2457 2549 2842 3454 4318 5207 6836 8
Size: 92 293 612 864 889 1629 43
Fragments arranged by size:
1629 1425 889 864 612 327 293 275
238 92 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4318 1537
Size: 275 2506 4090
Fragments arranged by size:
4090 2506 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3329 3914 4143 4801 1353
Size: 1976 585 229 658 3423
Fragments arranged by size:
3423 1976 658 585 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2357 3113 5458 5832 5978 387
Size: 1064 138 768 756 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 768 756 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2477 2718 3196 3269 3399 3714
Size: 393 461 384 241 478 73 130 315
Cuts at: 3714 3915 3989 4934 5531 6318 6586 1239
Size: 201 74 945 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 478 461 393 384
315 268 241 201 130 74 73
EcoNI CCTnn'n_nnAGG
Cuts at: 2121 2121
Size: 6871
EcoO109I rG'GnC_Cy
Cuts at: 2397 2624 3664 2397
Size: 227 1040 5604
Fragments arranged by size:
5604 1040 227
EcoRI G'AATT_C
Cuts at: 4180 4558 4180
Size: 378 6493
EcoRV GAT'ATC
Cuts at: 3121 3121
Size: 6871
FspI TGC'GCA
Cuts at: 5377 5377
Size: 6871
HaeII r_GCGC'y
Cuts at: 1678 3241 3527 6018 1678
Size: 1563 286 2491 2531
Fragments arranged by size:
2531 2491 1563 286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2873 2905 3491 3523 4052
Size: 32 135 32 1301 32 586 32 529
Cuts at: 4052 4084 5512 5544 5586 5618 1373
Size: 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 1301 586 529 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4215 4461 669
Size: 988 281 2277 246 3079
Fragments arranged by size:
3079 2277 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6871
HpaI GTT'AAC
Cuts at: 4461 4461
Size: 6871
KasI G'GCGC_C
Cuts at: 3523 3523
Size: 6871
KpnI G_GTAC'C
Cuts at: 4196 4196
Size: 6871
MfeI C'AATT_G
Cuts at: 2500 4470 2500
Size: 1970 4901
MluI A'CGCG_T
Cuts at: 6747 6747
Size: 6871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5985 6169 1305
Size: 4680 184 2007
Fragments arranged by size:
4680 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2844 3456 10
Size: 54 1425 343 1012 612 3425
Fragments arranged by size:
3425 1425 1012 612 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2334 2597 2756 3881 4829 5188
Size: 648 1002 166 263 159 1125 948 359
Cuts at: 5188 5347 518
Size: 159 2042
Fragments arranged by size:
2042 1125 1002 948 648 359 263 166
159 159
MspA1I CmG'CkG
Cuts at: 1606 2657 3473 4287 4964 6112 6357 1606
Size: 1051 816 814 677 1148 245 2120
Fragments arranged by size:
2120 1148 1051 816 814 677 245
NaeI GCC'GGC
Cuts at: 3717 3717
Size: 6871
NarI GG'CG_CC
Cuts at: 3524 3524
Size: 6871
NcoI C'CATG_G
Cuts at: 513 1065 2592 2947 3844 4167 513
Size: 552 1527 355 897 323 3217
Fragments arranged by size:
3217 1527 897 552 355 323
NdeI CA'TA_TG
Cuts at: 387 3940 387
Size: 3553 3318
NgoMIV G'CCGG_C
Cuts at: 3715 3715
Size: 6871
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6871
NotI GC'GGCC_GC
Cuts at: 4318 4318
Size: 6871
NspI r_CATG'y
Cuts at: 1132 1229 2514 2717 3055 4066 4229 5774 1132
Size: 97 1285 203 338 1011 163 1545 2229
Fragments arranged by size:
2229 1545 1285 1011 338 203 163 97
PasI CC'CwG_GG
Cuts at: 1348 2620 2627 1348
Size: 1272 7 5592
Fragments arranged by size:
5592 1272 7
PciI A'CATG_T
Cuts at: 3051 4062 5770 3051
Size: 1011 1708 4152
Fragments arranged by size:
4152 1708 1011
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2455 3826 1108
Size: 1347 1371 4153
Fragments arranged by size:
4153 1371 1347
PfoI T'CCnGG_A
Cuts at: 3246 3246
Size: 6871
PmeI GTTT'AAAC
Cuts at: 4175 4175
Size: 6871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4940 4972 6453 6485 4940
Size: 32 1481 32 5326
Fragments arranged by size:
5326 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6871
PsiI TTA'TAA
Cuts at: 4441 4441
Size: 6871
PspOMI G'GGCC_C
Cuts at: 2396 2623 3912 2396
Size: 227 1289 5355
Fragments arranged by size:
5355 1289 227
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2348 2380 3182 3214 2348
Size: 32 802 32 6005
Fragments arranged by size:
6005 802 32 32
PstI C_TGCA'G
Cuts at: 830 4223 830
Size: 3393 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6871
PvuII CAG'CTG
Cuts at: 3473 3473
Size: 6871
SalI G'TCGA_C
Cuts at: 1936 4213 1936
Size: 2277 4594
SapI GCTCTTCn'nnn_
Cuts at: 4143 4143
Size: 6871
SbfI CC_TGCA'GG
Cuts at: 4223 4223
Size: 6871
ScaI AGT'ACT
Cuts at: 4130 5119 6831 4130
Size: 989 1712 4170
Fragments arranged by size:
4170 1712 989
SexAI A'CCwGG_T
Cuts at: 2358 2358
Size: 6871
SfcI C'TryA_G
Cuts at: 826 1045 4219 5354 6035 6226 826
Size: 219 3174 1135 681 191 1471
Fragments arranged by size:
3174 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3525 3525
Size: 6871
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6871
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6871
SmaI CCC'GGG
Cuts at: 1872 4198 1872
Size: 2326 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2741 4975 5876 6138 820
Size: 197 926 798 2234 901 262 1553
Fragments arranged by size:
2234 1553 926 901 798 262 197
SnaBI TAC'GTA
Cuts at: 493 4662 493
Size: 4169 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6871
SphI G_CATG'C
Cuts at: 1132 2514 4229 1132
Size: 1382 1715 3774
Fragments arranged by size:
3774 1715 1382
SspI AAT'ATT
Cuts at: 5 52 4795 5
Size: 47 4743 2081
Fragments arranged by size:
4743 2081 47
StuI AGG'CCT
Cuts at: 1517 2228 2739 3863 1517
Size: 711 511 1124 4525
Fragments arranged by size:
4525 1124 711 511
StyI C'CwwG_G
Cuts at: 513 1065 2592 2947 3844 4167 4310 513
Size: 552 1527 355 897 323 143 3074
Fragments arranged by size:
3074 1527 897 552 355 323 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 2960 3345 4898 4915 5068 5253
Size: 1255 588 792 385 1553 17 153 185
Cuts at: 5253 5698 325
Size: 445 1498
Fragments arranged by size:
1553 1498 1255 792 588 445 385 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4128 5117 6829
Size: 274 80 33 51 1443 2151 989 1712
Cuts at: 6829 96
Size: 138
Fragments arranged by size:
2151 1712 1443 989 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 3606 3638 6831 6863 2079
Size: 32 1495 32 3193 32 2087
Fragments arranged by size:
3193 2087 1495 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6871
XbaI T'CTAG_A
Cuts at: 4207 4207
Size: 6871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2827 3169 3237 2827
Size: 342 68 6461
Fragments arranged by size:
6461 342 68
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6871
XmaI C'CCGG_G
Cuts at: 1870 4196 1870
Size: 2326 4545
XmnI GAAnn'nnTTC
Cuts at: 5000 5000
Size: 6871
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4679 276
Size: 53 83 186 936 3145 2468
Fragments arranged by size:
3145 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI
PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI
SfiI SrfI SwaI