Restriction map for
pFN21AA0834
(Circular) (Six-base) MAPSORT of: pFN21AA0834.seq Check: 857 from: 1 to: 6058
pFN21AA0834 6058 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2891 2891
Size: 6058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2093 2397 3401 1937
Size: 156 304 1004 4594
Fragments arranged by size:
4594 1004 304 156
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2459 4121 5505 5773 1632
Size: 827 1662 1384 268 1917
Fragments arranged by size:
1917 1662 1384 827 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 2892 3243 4957 5934 1203
Size: 1689 351 1714 977 1327
Fragments arranged by size:
1714 1689 1327 977 351
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 2671 3290 3801 5373 2671
Size: 619 511 1572 3356
Fragments arranged by size:
3356 1572 619 511
ApaLI G'TGCA_C
Cuts at: 2588 4115 5271 2588
Size: 1527 1156 3375
Fragments arranged by size:
3375 1527 1156
ApoI r'AATT_y
Cuts at: 1493 2333 3367 3593 3745 5854 1493
Size: 840 1034 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 840 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 1870
Size: 73 1440 4545
Fragments arranged by size:
4545 1440 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2043 3377 1391
Size: 652 1334 4072
Fragments arranged by size:
4072 1334 652
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3240 928
Size: 971 1341 3746
Fragments arranged by size:
3746 1341 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4249 4283 1903
Size: 34 50 34 2228 34 3678
Fragments arranged by size:
3678 2228 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 2124 2445 2739 1548
Size: 576 321 294 4867
Fragments arranged by size:
4867 576 321 294
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3223 4669 136
Size: 107 122 71 631 605 1551 1446 1525
Fragments arranged by size:
1551 1525 1446 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3107 3475 3107
Size: 368 5690
Bme1580I G_kGCm'C
Cuts at: 1790 2592 4119 5275 1790
Size: 802 1527 1156 2573
Fragments arranged by size:
2573 1527 1156 802
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3789 4747 442
Size: 416 53 83 399 24 2372 958 1753
Fragments arranged by size:
2372 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2832 3045 3312 4718 1239
Size: 1051 542 213 267 1406 2579
Fragments arranged by size:
2579 1406 1051 542 267 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3211 4183 5048 5346 3211
Size: 972 865 298 3923
Fragments arranged by size:
3923 972 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2709 4721 882
Size: 617 1210 2012 2219
Fragments arranged by size:
2219 2012 1210 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2798 2828 3122 3152 1178
Size: 30 165 30 1395 30 294 30 4084
Fragments arranged by size:
4084 1395 294 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2257 1186
Size: 1071 4987
BseYI C'CCAG_C
Cuts at: 2312 5261 5757 2312
Size: 2949 496 2613
Fragments arranged by size:
2949 2613 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2186 2592 4119 4204 5275 2186
Size: 406 1527 85 1071 2969
Fragments arranged by size:
2969 1527 1071 406 85
BsmI GAATG_Cn'
Cuts at: 2230 3569 3662 2230
Size: 1339 93 4626
Fragments arranged by size:
4626 1339 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2555 2555
Size: 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2186 2592 3377 4119 4204 5275
Size: 399 253 143 406 785 742 85 1071
Cuts at: 5275 1391
Size: 2174
Fragments arranged by size:
2174 1071 785 742 406 399 253 143
85
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 2539 3947 5677 2539
Size: 1408 1730 2920
Fragments arranged by size:
2920 1730 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 3222 3413 844
Size: 2047 331 191 3489
Fragments arranged by size:
3489 2047 331 191
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2032 4553 4727 65
Size: 1183 784 2521 174 1396
Fragments arranged by size:
2521 1396 1183 784 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3069 96
Size: 2973 3085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 3197 4118 5130 3197
Size: 921 1012 4125
Fragments arranged by size:
4125 1012 921
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3185 794
Size: 2391 3667
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 2827 1184
Size: 1643 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 2824 3228 2824
Size: 404 5654
BstYI r'GATC_y
Cuts at: 1972 2381 3388 3755 4140 4157 5609 5695 5707
Size: 409 1007 367 385 17 1452 86 12
Cuts at: 5707 6053 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1007 409 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2398 2398
Size: 6058
Bsu36I CC'TnA_GG
Cuts at: 2148 2148
Size: 6058
BtgI C'CryG_G
Cuts at: 513 1065 3221 513
Size: 552 2156 3350
Fragments arranged by size:
3350 2156 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 3574 4368 4388 3574
Size: 794 20 5244
Fragments arranged by size:
5244 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2869 2904 529
Size: 35 2305 35 3683
Fragments arranged by size:
3683 2305 35 35
DraI TTT'AAA
Cuts at: 2505 3362 2505
Size: 857 5201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3505 4394 6023
Size: 54 1425 50 275 18 1675 889 1629
Cuts at: 6023 8
Size: 43
Fragments arranged by size:
1675 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3988 1353
Size: 2635 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2290 2459 2832 3045 3312 4121 4718
Size: 393 658 169 373 213 267 809 597
Cuts at: 4718 5505 5773 1239
Size: 787 268 1524
Fragments arranged by size:
1524 809 787 658 597 393 373 268
267 213 169
EcoO109I rG'GnC_Cy
Cuts at: 3130 3130
Size: 6058
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2516 2516
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3122 3154 4699 4731 4773
Size: 32 135 32 1550 32 1545 32 42
Cuts at: 4773 4805 1373
Size: 32 2626
Fragments arranged by size:
2626 1550 1545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2493 748
Size: 1745 4313
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 5172 5356 1305
Size: 1536 2331 184 2007
Fragments arranged by size:
2331 2007 1536 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4236
Fragments arranged by size:
4236 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2074 2087 3226 3242 4016 4375 4534
Size: 648 908 13 1139 16 774 359 159
Cuts at: 4534 518
Size: 2042
Fragments arranged by size:
2042 1139 908 774 648 359 159 16
13
MspA1I CmG'CkG
Cuts at: 1606 3474 4151 5299 5544 1606
Size: 1868 677 1148 245 2120
Fragments arranged by size:
2120 1868 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5506
NdeI CA'TA_TG
Cuts at: 387 2353 387
Size: 1966 4092
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 2522 2896 3247 3304 3416 4961 1132
Size: 97 1293 374 351 57 112 1545 2229
Fragments arranged by size:
2229 1545 1293 374 351 112 97 57
PasI CC'CwG_GG
Cuts at: 1348 2624 1348
Size: 1276 4782
PciI A'CATG_T
Cuts at: 2892 3243 4957 2892
Size: 351 1714 3993
Fragments arranged by size:
3993 1714 351
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 3290 1108
Size: 1005 1177 3876
Fragments arranged by size:
3876 1177 1005
PfoI T'CCnGG_A
Cuts at: 2947 2947
Size: 6058
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3130 3130
Size: 6058
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2038 2457 3410 830
Size: 1208 419 953 3478
Fragments arranged by size:
3478 1208 953 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2034 2391 2453 3406 4541 5222 5413
Size: 219 989 357 62 953 1135 681 191
Cuts at: 5413 826
Size: 1471
Fragments arranged by size:
1471 1135 989 953 681 357 219 191
62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 3385 1872
Size: 1513 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3190 4162 5063 5325 820
Size: 197 926 1247 972 901 262 1553
Fragments arranged by size:
1553 1247 972 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3304 3416 1132
Size: 2172 112 3774
Fragments arranged by size:
3774 2172 112
SspI AAT'ATT
Cuts at: 5 52 2876 2985 3982 5
Size: 47 2824 109 997 2081
Fragments arranged by size:
2824 2081 997 109 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2556 2929 3497 513
Size: 552 1491 373 568 3074
Fragments arranged by size:
3074 1491 568 552 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 4085 4102 4255 4440 4885 325
Size: 1255 1185 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 1185 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 3069 4304
Size: 274 80 33 51 1443 630 462 1235
Cuts at: 4304 6016 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1235 630 462 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2798 2830 6018 6050 2798
Size: 32 3188 32 2806
Fragments arranged by size:
3188 2806 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 3383 1870
Size: 1513 4545
XmnI GAAnn'nnTTC
Cuts at: 4187 4187
Size: 6058
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI