Restriction map for
pFN21AA0830
(Circular) (Six-base) MAPSORT of: pFN21AA0830.seq Check: 7087 from: 1 to: 6205
pFN21AA0830 6205 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3094 3094
Size: 6205
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 3548 1937
Size: 1005 606 4594
Fragments arranged by size:
4594 1005 606
Acc65I G'GTAC_C
Cuts at: 3419 3526 3419
Size: 107 6098
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2750 3086 3284 4268 5652 5920 1632
Size: 1118 336 198 984 1384 268 1917
Fragments arranged by size:
1917 1384 1118 984 336 268 198
AfeI AGC'GCT
Cuts at: 2195 2195
Size: 6205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 5008
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6205
AlwNI CAG_nnn'CTG
Cuts at: 2651 2773 3948 5520 2651
Size: 122 1175 1572 3336
Fragments arranged by size:
3336 1572 1175 122
ApaLI G'TGCA_C
Cuts at: 4262 5418 4262
Size: 1156 5049
ApoI r'AATT_y
Cuts at: 1493 2488 3514 3740 3892 6001 1493
Size: 995 1026 226 152 2109 1697
Fragments arranged by size:
2109 1697 1026 995 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2340 3038 3111 4759 160
Size: 872 1308 698 73 1648 1606
Fragments arranged by size:
1648 1606 1308 872 698 73
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 2724 3530 1870
Size: 73 274 507 806 4545
Fragments arranged by size:
4545 806 507 274 73
BamHI G'GATC_C
Cuts at: 3246 3535 3902 3246
Size: 289 367 5549
Fragments arranged by size:
5549 367 289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2273 3419 3526 4981
Size: 325 217 627 222 264 1146 107 1455
Cuts at: 4981 618
Size: 1842
Fragments arranged by size:
1842 1455 1146 627 325 264 222 217
107
BanII G_rGCy'C
Cuts at: 1391 3146 3238 3524 1391
Size: 1755 92 286 4072
Fragments arranged by size:
4072 1755 286 92
BbeI G_GCGC'C
Cuts at: 2277 2277
Size: 6205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2592 928
Size: 971 693 4541
Fragments arranged by size:
4541 971 693
BbvCI CC'TCA_GC
Cuts at: 2790 2790
Size: 6205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2023 2057 4396 4430 1903
Size: 34 86 34 2339 34 3678
Fragments arranged by size:
3678 2339 86 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2140 3384 4816
Size: 107 122 71 631 605 468 1244 1432
Cuts at: 4816 136
Size: 1525
Fragments arranged by size:
1525 1432 1244 631 605 468 122 107
71
BglII A'GATC_T
Cuts at: 2166 2761 6200 2166
Size: 595 3439 2171
Fragments arranged by size:
3439 2171 595
BlpI GC'TnA_GC
Cuts at: 2190 3145 3622 2190
Size: 955 477 4773
Fragments arranged by size:
4773 955 477
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2616 4266 5422 1790
Size: 222 604 1650 1156 2573
Fragments arranged by size:
2573 1650 1156 604 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3383 3936 4894
Size: 416 53 83 399 24 1966 553 958
Cuts at: 4894 442
Size: 1753
Fragments arranged by size:
1966 1753 958 553 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2961 2961
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2709 2940 4865 1239
Size: 1470 231 1925 2579
Fragments arranged by size:
2579 1925 1470 231
Bpu10I CC'TnA_GC
Cuts at: 2450 2790 2450
Size: 340 5865
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 3194 4330 5195 5493 2348
Size: 846 1136 865 298 3060
Fragments arranged by size:
3060 1136 865 846 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4868 882
Size: 617 3369 2219
Fragments arranged by size:
3369 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2161 3996 493
Size: 711 957 1835 2702
Fragments arranged by size:
2702 1835 957 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 4012 4394 5029
Size: 53 83 186 936 741 1738 382 635
Cuts at: 5029 275
Size: 1451
Fragments arranged by size:
1738 1451 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3243 4638 5036 5310 5457 5868 3243
Size: 1395 398 274 147 411 3580
Fragments arranged by size:
3580 1395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 4924
Fragments arranged by size:
4924 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2096 2345 2706 3230 1186
Size: 910 249 361 524 4161
Fragments arranged by size:
4161 910 524 361 249
BseYI C'CCAG_C
Cuts at: 2029 2051 2697 5408 5904 2029
Size: 22 646 2711 496 2330
Fragments arranged by size:
2711 2330 646 496 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5632
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 3655 4416 5444 1540
Size: 275 186 150 1504 761 1028 2301
Fragments arranged by size:
2301 1504 1028 761 275 186 150
BsiHKAI G_wGCw'C
Cuts at: 3210 4266 4351 5422 3210
Size: 1056 85 1071 3993
Fragments arranged by size:
3993 1071 1056 85
BsmI GAATG_Cn'
Cuts at: 3192 3716 3809 3192
Size: 524 93 5588
Fragments arranged by size:
5588 524 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2616 3146 3210 3238 3524 4266
Size: 399 222 604 530 64 28 286 742
Cuts at: 4266 4351 5422 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 530 399 286 222
85 64 28
BspEI T'CCGG_A
Cuts at: 3243 5868 3243
Size: 2625 3580
BspHI T'CATG_A
Cuts at: 4094 5824 4094
Size: 1730 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3094 3560 844
Size: 2250 466 3489
Fragments arranged by size:
3489 2250 466
BsrBI CCG'CTC
Cuts at: 1394 1823 4092 1394
Size: 429 2269 3507
Fragments arranged by size:
3507 2269 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3006 3366 4700 4874 65
Size: 1183 1758 360 1334 174 1396
Fragments arranged by size:
1758 1396 1334 1183 360 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2067 2090 4849 5036 1700
Size: 110 257 23 2759 187 2869
Fragments arranged by size:
2869 2759 257 187 110 23
BsrGI T'GTAC_A
Cuts at: 96 3330 96
Size: 3234 2971
BssHII G'CGCG_C
Cuts at: 1925 2015 2176 1925
Size: 90 161 5954
Fragments arranged by size:
5954 161 90
BssSI C'ACGA_G
Cuts at: 2072 4265 5277 2072
Size: 2193 1012 3000
Fragments arranged by size:
3000 2193 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2166 2761 3246 3535 3902 4287 4304 5756
Size: 194 595 485 289 367 385 17 1452
Cuts at: 5756 5842 5854 6200 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 595 485 385 367 346 289
194 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2703 3336 513
Size: 552 939 699 633 3382
Fragments arranged by size:
3382 939 699 633 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2299 487
Size: 683 56 1073 4393
Fragments arranged by size:
4393 1073 683 56
BtsI GCAGTG_nn'
Cuts at: 2461 2584 3395 3408 3721 4515 4535 2461
Size: 123 811 13 313 794 20 4131
Fragments arranged by size:
4131 811 794 313 123 20 13
ClaI AT'CG_AT
Cuts at: 1554 2307 1554
Size: 753 5452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DraIII CAC_nnn'GTG
Cuts at: 2573 2573
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5212 809
Size: 507 3896 1802
Fragments arranged by size:
3896 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2148 2469 3168
Size: 54 1425 50 275 18 318 321 699
Cuts at: 3168 3219 3315 3652 4541 6170 8
Size: 51 96 337 889 1629 43
Fragments arranged by size:
1629 1425 889 699 337 321 318 275
96 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 3652 1537
Size: 275 336 1504 4090
Fragments arranged by size:
4090 1504 336 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3174 4135 1353
Size: 692 1129 961 3423
Fragments arranged by size:
3423 1129 961 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4792 5166 5312 387
Size: 1064 138 3203 374 146 1280
Fragments arranged by size:
3203 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2709 2750 2940 3086 3284 4268 4865
Size: 393 1077 41 190 146 198 984 597
Cuts at: 4865 5652 5920 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1077 984 787 597 393 268 198
190 146 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2691 3437 2330
Size: 361 746 5098
Fragments arranged by size:
5098 746 361
EcoO109I rG'GnC_Cy
Cuts at: 2127 2127
Size: 6205
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2197 2277 3345 5352 1678
Size: 519 80 1068 2007 2531
Fragments arranged by size:
2531 2007 1068 519 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2896 2928 4846 4878 4920
Size: 32 135 32 1324 32 1918 32 42
Cuts at: 4920 4952 1373
Size: 32 2626
Fragments arranged by size:
2626 1918 1324 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 3549 3795 669
Size: 988 281 925 686 246 3079
Fragments arranged by size:
3079 988 925 686 281 246
HindIII A'AGCT_T
Cuts at: 748 3026 748
Size: 2278 3927
HpaI GTT'AAC
Cuts at: 2863 3795 2863
Size: 932 5273
KasI G'GCGC_C
Cuts at: 2273 2273
Size: 6205
KpnI G_GTAC'C
Cuts at: 3423 3530 3423
Size: 107 6098
MfeI C'AATT_G
Cuts at: 3351 3804 3351
Size: 453 5752
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5319 5503 1305
Size: 4014 184 2007
Fragments arranged by size:
4014 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 3170 3221 3317 10
Size: 54 1425 343 639 699 51 96 2898
Fragments arranged by size:
2898 1425 699 639 343 96 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2231 2363 4163 4522 4681 518
Size: 648 1065 132 1800 359 159 2042
Fragments arranged by size:
2042 1800 1065 648 359 159 132
MspA1I CmG'CkG
Cuts at: 1606 2794 3621 4298 5446 5691 1606
Size: 1188 827 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 827 677 245
NaeI GCC'GGC
Cuts at: 2069 2092 2069
Size: 23 6182
NarI GG'CG_CC
Cuts at: 2274 2274
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 2004 2703 3336 513
Size: 552 939 699 633 3382
Fragments arranged by size:
3382 939 699 633 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NgoMIV G'CCGG_C
Cuts at: 2067 2090 2067
Size: 23 6182
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 3563 5108 1132
Size: 97 2334 1545 2229
Fragments arranged by size:
2334 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3424 1348
Size: 2076 4129
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2573 1108
Size: 1465 4740
PfoI T'CCnGG_A
Cuts at: 2514 2850 2871 2514
Size: 336 21 5848
Fragments arranged by size:
5848 336 21
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PmlI CAC'GTG
Cuts at: 2161 2161
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2127 2127
Size: 6205
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2228 2260 2874 2906 2228
Size: 32 614 32 5527
Fragments arranged by size:
5527 614 32 32
PstI C_TGCA'G
Cuts at: 830 3557 830
Size: 2727 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2794 2794
Size: 6205
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SanDI GG'GwC_CC
Cuts at: 2127 2127
Size: 6205
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2209 2623 3553 4688 5369 5560 826
Size: 219 1164 414 930 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 930 681 414 219 191
SfoI GGC'GCC
Cuts at: 2275 2275
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 2219 2726 3532 1872
Size: 347 507 806 4545
Fragments arranged by size:
4545 806 507 347
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 3209 4309 5210 5472 820
Size: 197 926 384 882 1100 901 262 1553
Fragments arranged by size:
1553 1100 926 901 882 384 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3563 1132
Size: 2431 3774
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 2004 2703 3336 3379 3644 513
Size: 552 939 699 633 43 265 3074
Fragments arranged by size:
3074 939 699 633 552 265 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 4232 4249 4402 4587 5032 325
Size: 1255 1836 816 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3330 4451
Size: 274 80 33 51 1443 258 1095 1121
Cuts at: 4451 6163 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1121 1095 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6165 6197 6165
Size: 32 6173
Tth111I GACn'n_nGTC
Cuts at: 1316 2506 1316
Size: 1190 5015
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 2217 2724 3530 1870
Size: 347 507 806 4545
Fragments arranged by size:
4545 806 507 347
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PspOMI PspXI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI XcmI