Restriction map for
pFN21AA0822
(Circular) (Six-base) MAPSORT of: pFN21AA0822.seq Check: 1512 from: 1 to: 9448
pFN21AA0822 9448 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5404 5404
Size: 9448
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3298 7258 278
Size: 53 83 186 936 1762 3960 2468
Fragments arranged by size:
3960 2468 1762 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2238 4526 5737 6218 6638 6791 1937
Size: 301 2288 1211 481 420 153 4594
Fragments arranged by size:
4594 2288 1211 481 420 301 153
Acc65I G'GTAC_C
Cuts at: 6769 6769
Size: 9448
AclI AA'CG_TT
Cuts at: 1563 7575 7948 1563
Size: 6012 373 3063
Fragments arranged by size:
6012 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2896 3335 3877 4268 5478 6137 7511
Size: 756 508 439 542 391 1210 659 1374
Cuts at: 7511 8895 9163 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1374 1210 756 659 542 508
439 391 268
AflII C'TTAA_G
Cuts at: 820 1017 4018 820
Size: 197 3001 6250
Fragments arranged by size:
6250 3001 197
AflIII A'CryG_T
Cuts at: 1203 8347 9324 1203
Size: 7144 977 1327
Fragments arranged by size:
7144 1327 977
AgeI A'CCGG_T
Cuts at: 8279 8279
Size: 9448
AhdI GACnn_n'nnGTC
Cuts at: 8177 8177
Size: 9448
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 2780
Size: 32 9416
AlwNI CAG_nnn'CTG
Cuts at: 2618 5095 6022 6326 6682 7191 8763 2618
Size: 2477 927 304 356 509 1572 3303
Fragments arranged by size:
3303 2477 1572 927 509 356 304
ApaLI G'TGCA_C
Cuts at: 7505 8661 7505
Size: 1156 8292
ApoI r'AATT_y
Cuts at: 1493 2110 2348 3154 3208 3267 3422 4154 5592
Size: 617 238 806 54 59 155 732 1438
Cuts at: 5592 5692 6407 6757 6983 7135 9244 1493
Size: 100 715 350 226 152 2109 1697
Fragments arranged by size:
2109 1697 1438 806 732 715 617 350
238 226 155 152 100 59 54
AseI AT'TA_AT
Cuts at: 160 1032 2556 4272 4487 8002 160
Size: 872 1524 1716 215 3515 1606
Fragments arranged by size:
3515 1716 1606 1524 872 215
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9448
AvaI C'yCGr_G
Cuts at: 1870 1943 6233 6773 1870
Size: 73 4290 540 4545
Fragments arranged by size:
4545 4290 540 73
AvrII C'CTAG_G
Cuts at: 4195 4195
Size: 9448
BamHI G'GATC_C
Cuts at: 3826 5578 6778 7145 3826
Size: 1752 1200 367 6129
Fragments arranged by size:
6129 1752 1200 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6293 6769 8224 618
Size: 325 217 627 4506 476 1455 1842
Fragments arranged by size:
4506 1842 1455 627 476 325 217
BanII G_rGCy'C
Cuts at: 1391 3977 5038 6465 6666 6767 1391
Size: 2586 1061 1427 201 101 4072
Fragments arranged by size:
4072 2586 1427 1061 201 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2774 2902 5116 5172 6137 928
Size: 971 875 128 2214 56 965 4239
Fragments arranged by size:
4239 2214 971 965 875 128 56
BbvCI CC'TCA_GC
Cuts at: 6050 6066 6327 6050
Size: 16 261 9171
Fragments arranged by size:
9171 261 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7639 7673 1903
Size: 34 5702 34 3678
Fragments arranged by size:
5702 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7340 8550 7340
Size: 1210 8238
BclI T'GATC_A
Cuts at: 1548 3349 4632 1548
Size: 1801 1283 6364
Fragments arranged by size:
6364 1801 1283
BfrBI ATG'CAT
Cuts at: 2638 2723 3164 4712 2638
Size: 85 441 1548 7374
Fragments arranged by size:
7374 1548 441 85
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6266 8059 136
Size: 107 122 71 631 605 4594 1793 1525
Fragments arranged by size:
4594 1793 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2870 3505 9443 2870
Size: 635 5938 2875
Fragments arranged by size:
5938 2875 635
BlpI GC'TnA_GC
Cuts at: 6865 6865
Size: 9448
Bme1580I G_kGCm'C
Cuts at: 1790 6298 7509 8665 1790
Size: 4508 1211 1156 2573
Fragments arranged by size:
4508 2573 1211 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9448
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2872 3895 4826
Size: 416 53 83 399 24 1455 1023 931
Cuts at: 4826 6191 7179 8137 442
Size: 1365 988 958 1753
Fragments arranged by size:
1753 1455 1365 1023 988 958 931 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3755
Size: 9448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3402 5916 5978 6044 6449 6665 6680 8108
Size: 2163 2514 62 66 405 216 15 1428
Cuts at: 8108 1239
Size: 2579
Fragments arranged by size:
2579 2514 2163 1428 405 216 66 62
15
Bpu10I CC'TnA_GC
Cuts at: 6050 6066 6173 6327 6050
Size: 16 107 154 9171
Fragments arranged by size:
9171 154 107 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6083 7573 8438 8736 6083
Size: 1490 865 298 6795
Fragments arranged by size:
6795 1490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3336 8111 882
Size: 617 1837 4775 2219
Fragments arranged by size:
4775 2219 1837 617
BsaAI yAC'GTr
Cuts at: 493 1204 3355 5248 7239 493
Size: 711 2151 1893 1991 2702
Fragments arranged by size:
2702 2151 1991 1893 711
BsaBI GATnn'nnATC
Cuts at: 3381 4185 6705 9442 3381
Size: 804 2520 2737 3387
Fragments arranged by size:
3387 2737 2520 804
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3295 7255 7637 8272
Size: 53 83 186 936 1762 3960 382 635
Cuts at: 8272 275
Size: 1451
Fragments arranged by size:
3960 1762 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3683 5057 6124 7881 8279 8553 8700 9111 3683
Size: 1374 1067 1757 398 274 147 411 4020
Fragments arranged by size:
4020 1757 1374 1067 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2594 2624 1178
Size: 30 165 30 1191 30 8002
Fragments arranged by size:
8002 1191 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2615 3002 4563 6511 6632 6710 6719 1186
Size: 1429 387 1561 1948 121 78 9 3915
Fragments arranged by size:
3915 1948 1561 1429 387 121 78 9
BseYI C'CCAG_C
Cuts at: 3126 5043 6682 8651 9147 3126
Size: 1917 1639 1969 496 3427
Fragments arranged by size:
3427 1969 1917 1639 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 8875
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6222 6898 7659 8687 1540
Size: 275 186 4221 676 761 1028 2301
Fragments arranged by size:
4221 2301 1028 761 676 275 186
BsiHKAI G_wGCw'C
Cuts at: 2457 4686 5038 6666 7509 7594 8665 2457
Size: 2229 352 1628 843 85 1071 3240
Fragments arranged by size:
3240 2229 1628 1071 843 352 85
BsmI GAATG_Cn'
Cuts at: 2442 2638 6959 7052 2442
Size: 196 4321 93 4838
Fragments arranged by size:
4838 4321 196 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2457 3977 4686 5038 6298 6465 6666
Size: 399 667 1520 709 352 1260 167 201
Cuts at: 6666 6767 7509 7594 8665 1391
Size: 101 742 85 1071 2174
Fragments arranged by size:
2174 1520 1260 1071 742 709 667 399
352 201 167 101 85
BspEI T'CCGG_A
Cuts at: 5057 9111 5057
Size: 4054 5394
BspHI T'CATG_A
Cuts at: 3394 5238 7337 9067 3394
Size: 1844 2099 1730 3775
Fragments arranged by size:
3775 2099 1844 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 5404 6432 6640 6803 844
Size: 1450 3110 1028 208 163 3489
Fragments arranged by size:
3489 3110 1450 1028 208 163
BsrBI CCG'CTC
Cuts at: 1394 1823 5940 7335 1394
Size: 429 4117 1395 3507
Fragments arranged by size:
4117 3507 1395 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2114 4284 4813 4998 5724 7943 8117
Size: 1183 866 2170 529 185 726 2219 174
Cuts at: 8117 65
Size: 1396
Fragments arranged by size:
2219 2170 1396 1183 866 726 529 185
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2993 8092 8279 1700
Size: 110 1183 5099 187 2869
Fragments arranged by size:
5099 2869 1183 187 110
BsrGI T'GTAC_A
Cuts at: 96 3051 5473 96
Size: 2955 2422 4071
Fragments arranged by size:
4071 2955 2422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9448
BssSI C'ACGA_G
Cuts at: 5800 7508 8520 5800
Size: 1708 1012 6728
Fragments arranged by size:
6728 1708 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4808 794
Size: 4014 5434
BstBI TT'CG_AA
Cuts at: 5163 5355 8252 5163
Size: 192 2897 6359
Fragments arranged by size:
6359 2897 192
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9448
BstXI CCAn_nnnn'nTGG
Cuts at: 2194 2194
Size: 9448
BstYI r'GATC_y
Cuts at: 1972 2870 3505 3826 4174 5578 6477 6778 7145
Size: 898 635 321 348 1404 899 301 367
Cuts at: 7145 7530 7547 8999 9085 9097 9443 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1404 899 898 635 385 367
348 346 321 301 86 17 12
BstZ17I GTA'TAC
Cuts at: 2239 4527 2239
Size: 2288 7160
Bsu36I CC'TnA_GG
Cuts at: 5979 6482 5979
Size: 503 8945
BtgI C'CryG_G
Cuts at: 513 1065 2187 4530 513
Size: 552 1122 2343 5431
Fragments arranged by size:
5431 2343 1122 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8709
Fragments arranged by size:
8709 683 56
BtsI GCAGTG_nn'
Cuts at: 2177 3537 3575 4478 6964 7758 7778 2177
Size: 1360 38 903 2486 794 20 3847
Fragments arranged by size:
3847 2486 1360 903 794 38 20
ClaI AT'CG_AT
Cuts at: 1554 5016 1554
Size: 3462 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5389 5424 529
Size: 35 4825 35 4553
Fragments arranged by size:
4825 4553 35 35
DraI TTT'AAA
Cuts at: 6752 6752
Size: 9448
DraIII CAC_nnn'GTG
Cuts at: 2593 4767 2593
Size: 2174 7274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3294 8455 809
Size: 507 1978 5161 1802
Fragments arranged by size:
5161 1978 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6348 6895 7784
Size: 54 1425 50 275 18 4518 547 889
Cuts at: 7784 9413 8
Size: 1629 43
Fragments arranged by size:
4518 1629 1425 889 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6895 1537
Size: 275 5083 4090
Fragments arranged by size:
5083 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 2547 3896 3982 4366 4518 5538 5753
Size: 653 541 1349 86 384 152 1020 215
Cuts at: 5753 6735 7378 1353
Size: 982 643 3423
Fragments arranged by size:
3423 1349 1020 982 653 643 541 384
215 152 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3944 8035 8409 8555 387
Size: 1064 138 2355 4091 374 146 1280
Fragments arranged by size:
4091 2355 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5036 6664 5036
Size: 1628 7820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2896 3335 3402 3877 4268 5478
Size: 393 756 508 439 67 475 391 1210
Cuts at: 5478 5916 5978 6044 6137 6449 6665 6680 7511
Size: 438 62 66 93 312 216 15 831
Cuts at: 7511 8108 8895 9163 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1210 831 787 756 597 508 475
439 438 393 391 312 268 216 93
67 66 62 15
EcoNI CCTnn'n_nnAGG
Cuts at: 4194 6647 6734 4194
Size: 2453 87 6908
Fragments arranged by size:
6908 2453 87
EcoRI G'AATT_C
Cuts at: 2110 2348 3154 3422 5692 6757 7135 2110
Size: 238 806 268 2270 1065 378 4423
Fragments arranged by size:
4423 2270 1065 806 378 268 238
EcoRV GAT'ATC
Cuts at: 4013 4013
Size: 9448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4136 6181 4136
Size: 2045 7403
FspI TGC'GCA
Cuts at: 4423 7954 4423
Size: 3531 5917
HaeII r_GCGC'y
Cuts at: 1678 3416 5096 8595 1678
Size: 1738 1680 3499 2531
Fragments arranged by size:
3499 2531 1738 1680
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2594 2626 3997 4029 4401
Size: 32 135 32 1022 32 1371 32 372
Cuts at: 4401 4433 4727 4759 5563 5595 6200 6232 6336
Size: 32 294 32 804 32 605 32 104
Cuts at: 6336 6368 8089 8121 8163 8195 1373
Size: 32 1721 32 42 32 2626
Fragments arranged by size:
2626 1721 1371 1022 804 605 372 294
135 104 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2033 3573 6219 6792 7038 669
Size: 988 281 95 1540 2646 573 246 3079
Fragments arranged by size:
3079 2646 1540 988 573 281 246 95
HindIII A'AGCT_T
Cuts at: 748 3088 3641 4021 6573 748
Size: 2340 553 380 2552 3623
Fragments arranged by size:
3623 2552 2340 553 380
HpaI GTT'AAC
Cuts at: 7038 7038
Size: 9448
KpnI G_GTAC'C
Cuts at: 6773 6773
Size: 9448
MfeI C'AATT_G
Cuts at: 4425 7047 4425
Size: 2622 6826
MluI A'CGCG_T
Cuts at: 9324 9324
Size: 9448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3783 6415 8562 8746 1305
Size: 2478 2632 2147 184 2007
Fragments arranged by size:
2632 2478 2147 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6350 10
Size: 54 1425 343 4518 3108
Fragments arranged by size:
4518 3108 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2478 4763 5003 5237 5885 7406 7765
Size: 648 1312 2285 240 234 648 1521 359
Cuts at: 7765 7924 518
Size: 159 2042
Fragments arranged by size:
2285 2042 1521 1312 648 648 359 240
234 159
MspA1I CmG'CkG
Cuts at: 1606 5733 6115 6864 7541 8689 8934 1606
Size: 4127 382 749 677 1148 245 2120
Fragments arranged by size:
4127 2120 1148 749 677 382 245
NcoI C'CATG_G
Cuts at: 513 1065 2187 4530 513
Size: 552 1122 2343 5431
Fragments arranged by size:
5431 2343 1122 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9448
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9448
NotI GC'GGCC_GC
Cuts at: 6895 6895
Size: 9448
NsiI A_TGCA'T
Cuts at: 2640 2725 3166 4714 2640
Size: 85 441 1548 7374
Fragments arranged by size:
7374 1548 441 85
NspI r_CATG'y
Cuts at: 1132 1229 4806 5173 6806 8351 1132
Size: 97 3577 367 1633 1545 2229
Fragments arranged by size:
3577 2229 1633 1545 367 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9448
PciI A'CATG_T
Cuts at: 8347 8347
Size: 9448
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3818 3907 5915 6316 1108
Size: 2710 89 2008 401 4240
Fragments arranged by size:
4240 2710 2008 401 89
PfoI T'CCnGG_A
Cuts at: 4594 6657 4594
Size: 2063 7385
PmeI GTTT'AAAC
Cuts at: 6752 6752
Size: 9448
PmlI CAC'GTG
Cuts at: 3355 3355
Size: 9448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4341 4373 7517 7549 9030 9062 4341
Size: 32 3144 32 1481 32 4727
Fragments arranged by size:
4727 3144 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9448
PsiI TTA'TAA
Cuts at: 2666 2867 4749 5025 6537 7018 2666
Size: 201 1882 276 1512 481 5096
Fragments arranged by size:
5096 1882 1512 481 276 201
PstI C_TGCA'G
Cuts at: 830 6108 6800 830
Size: 5278 692 3478
Fragments arranged by size:
5278 3478 692
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9448
PvuII CAG'CTG
Cuts at: 5733 6115 5733
Size: 382 9066
SacI G_AGCT'C
Cuts at: 5038 6666 5038
Size: 1628 7820
SalI G'TCGA_C
Cuts at: 1936 6217 6790 1936
Size: 4281 573 4594
Fragments arranged by size:
4594 4281 573
SapI GCTCTTCn'nnn_
Cuts at: 2547 3982 6735 2547
Size: 1435 2753 5260
Fragments arranged by size:
5260 2753 1435
SbfI CC_TGCA'GG
Cuts at: 6800 6800
Size: 9448
ScaI AGT'ACT
Cuts at: 7696 9408 7696
Size: 1712 7736
SfcI C'TryA_G
Cuts at: 826 1045 6104 6796 7931 8612 8803 826
Size: 219 5059 692 1135 681 191 1471
Fragments arranged by size:
5059 1471 1135 692 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 6266 6266
Size: 9448
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9448
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9448
SmaI CCC'GGG
Cuts at: 1872 6775 1872
Size: 4903 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4018 6098 7552 8453 8715 820
Size: 197 926 2075 2080 1454 901 262 1553
Fragments arranged by size:
2080 2075 1553 1454 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7239 493
Size: 6746 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9448
SphI G_CATG'C
Cuts at: 1132 4806 6806 1132
Size: 3674 2000 3774
Fragments arranged by size:
3774 3674 2000
SspI AAT'ATT
Cuts at: 5 52 7372 5
Size: 47 7320 2081
Fragments arranged by size:
7320 2081 47
StuI AGG'CCT
Cuts at: 1517 2762 1517
Size: 1245 8203
StyI C'CwwG_G
Cuts at: 513 1065 2187 3072 3427 3952 4195 4530 6669
Size: 552 1122 885 355 525 243 335 2139
Cuts at: 6669 6887 513
Size: 218 3074
Fragments arranged by size:
3074 2139 1122 885 552 525 355 335
243 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5028 6323 6357 6674 7475 7492 7645
Size: 1255 3448 1295 34 317 801 17 153
Cuts at: 7645 7830 8275 325
Size: 185 445 1498
Fragments arranged by size:
3448 1498 1295 1255 801 445 317 185
153 34 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3051 4303 4885
Size: 274 80 33 51 1443 1074 1252 582
Cuts at: 4885 4907 5112 5473 6621 7694 9406 96
Size: 22 205 361 1148 1073 1712 138
Fragments arranged by size:
1712 1443 1252 1148 1074 1073 582 361
274 205 138 80 51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 9408 9440 9408
Size: 32 9416
Tth111I GACn'n_nGTC
Cuts at: 1316 6136 1316
Size: 4820 4628
XbaI T'CTAG_A
Cuts at: 6784 6784
Size: 9448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6313 6313
Size: 9448
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9448
XmaI C'CCGG_G
Cuts at: 1870 6773 1870
Size: 4903 4545
XmnI GAAnn'nnTTC
Cuts at: 2984 4826 5655 7577 2984
Size: 1842 829 1922 4855
Fragments arranged by size:
4855 1922 1842 829
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3296 7256 276
Size: 53 83 186 936 1762 3960 2468
Fragments arranged by size:
3960 2468 1762 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbeI BsiWI BsmBI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI
SfoI SrfI SwaI