Restriction map for
pFN21AA0816
(Circular) (Six-base) MAPSORT of: pFN21AA0816.seq Check: 6181 from: 1 to: 10411
pFN21AA0816 10411 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3349 3349
Size: 10411
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8221 278
Size: 53 83 186 936 6685 2468
Fragments arranged by size:
6685 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2203 3524 5012 5268 5418 7754 1937
Size: 266 1321 1488 256 150 2336 4594
Fragments arranged by size:
4594 2336 1488 1321 266 256 150
Acc65I G'GTAC_C
Cuts at: 3701 5224 6444 6958 7732 3701
Size: 1523 1220 514 774 6380
Fragments arranged by size:
6380 1523 1220 774 514
AclI AA'CG_TT
Cuts at: 1563 8538 8911 1563
Size: 6975 373 3063
Fragments arranged by size:
6975 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 6047 6305 8474 9858 10126 1632
Size: 4415 258 2169 1384 268 1917
Fragments arranged by size:
4415 2169 1917 1384 268 258
AfeI AGC'GCT
Cuts at: 4000 4915 4000
Size: 915 9496
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10214
AflIII A'CryG_T
Cuts at: 1203 2102 3177 4362 4620 9310 10287 1203
Size: 899 1075 1185 258 4690 977 1327
Fragments arranged by size:
4690 1327 1185 1075 977 899 258
AgeI A'CCGG_T
Cuts at: 5755 9242 5755
Size: 3487 6924
AhdI GACnn_n'nnGTC
Cuts at: 7537 9140 7537
Size: 1603 8808
AleI CACnn'nnGTG
Cuts at: 1166 4209 6131 6212 1166
Size: 3043 1922 81 5365
Fragments arranged by size:
5365 3043 1922 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5126 1184
Size: 3942 6469
AlwNI CAG_nnn'CTG
Cuts at: 2956 3323 3364 3661 8154 9726 2956
Size: 367 41 297 4493 1572 3641
Fragments arranged by size:
4493 3641 1572 367 297 41
ApaI G_GGCC'C
Cuts at: 3634 3634
Size: 10411
ApaLI G'TGCA_C
Cuts at: 7116 8468 9624 7116
Size: 1352 1156 7903
Fragments arranged by size:
7903 1352 1156
ApoI r'AATT_y
Cuts at: 1493 4007 4787 7720 7946 8098 10207 1493
Size: 2514 780 2933 226 152 2109 1697
Fragments arranged by size:
2933 2514 2109 1697 780 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8965 160
Size: 872 7933 1606
Fragments arranged by size:
7933 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10411
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2325 3601 3672 4350 4587 5511
Size: 73 129 253 1276 71 678 237 924
Cuts at: 5511 5947 7736 1870
Size: 436 1789 4545
Fragments arranged by size:
4545 1789 1276 924 678 436 253 237
129 73 71
AvrII C'CTAG_G
Cuts at: 5632 6971 5632
Size: 1339 9072
BamHI G'GATC_C
Cuts at: 3477 7741 8108 3477
Size: 4264 367 5780
Fragments arranged by size:
5780 4264 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 3596 3701 3831 5224
Size: 325 217 627 249 1560 105 130 1393
Cuts at: 5224 5729 6444 6958 7732 9187 618
Size: 505 715 514 774 1455 1842
Fragments arranged by size:
1842 1560 1455 1393 774 715 627 514
505 325 249 217 130 105
BanII G_rGCy'C
Cuts at: 1391 3542 3634 4457 4649 5391 6156 7001 7730
Size: 2151 92 823 192 742 765 845 729
Cuts at: 7730 1391
Size: 4072
Fragments arranged by size:
4072 2151 845 823 765 742 729 192
92
BbeI G_GCGC'C
Cuts at: 2040 3835 2040
Size: 1795 8616
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3442 3960 4784 4978 6047 6293 7532
Size: 971 1543 518 824 194 1069 246 1239
Cuts at: 7532 928
Size: 3807
Fragments arranged by size:
3807 1543 1239 1069 971 824 518 246
194
BbvCI CC'TCA_GC
Cuts at: 2554 6819 2554
Size: 4265 6146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8602 8636 1903
Size: 34 6665 34 3678
Fragments arranged by size:
6665 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4432 8303 9513 4432
Size: 3871 1210 5330
Fragments arranged by size:
5330 3871 1210
BclI T'GATC_A
Cuts at: 1548 6399 1548
Size: 4851 5560
BfrBI ATG'CAT
Cuts at: 7521 7521
Size: 10411
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2361 3150 3292
Size: 107 122 71 631 605 689 789 142
Cuts at: 3292 3546 4182 4461 5100 6015 9022 136
Size: 254 636 279 639 915 3007 1525
Fragments arranged by size:
3007 1525 915 789 689 639 636 631
605 279 254 142 122 107 71
BglII A'GATC_T
Cuts at: 10406 10406
Size: 10411
BlpI GC'TnA_GC
Cuts at: 2490 3855 7828 2490
Size: 1365 3973 5073
Fragments arranged by size:
5073 3973 1365
Bme1580I G_kGCm'C
Cuts at: 1790 3103 3177 3634 4216 5920 7120 8472 9628
Size: 1313 74 457 582 1704 1200 1352 1156
Cuts at: 9628 1790
Size: 2573
Fragments arranged by size:
2573 1704 1352 1313 1200 1156 582 457
74
BmgBI CAC'GTC
Cuts at: 1318 4318 7059 1318
Size: 3000 2741 4670
Fragments arranged by size:
4670 3000 2741
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2140 3537 3730
Size: 416 53 83 399 24 723 1397 193
Cuts at: 3730 3853 4143 4351 4645 4768 5000 5569 6322
Size: 123 290 208 294 123 232 569 753
Cuts at: 6322 6472 6595 6995 8142 9100 442
Size: 150 123 400 1147 958 1753
Fragments arranged by size:
1753 1397 1147 958 753 723 569 416
400 399 294 290 232 208 193 150
123 123 123 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10411
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 10411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2603 3425 3554 3683 3733 6064 7085 7131
Size: 1364 822 129 129 50 2331 1021 46
Cuts at: 7131 7623 9071 1239
Size: 492 1448 2579
Fragments arranged by size:
2579 2331 1448 1364 1021 822 492 129
129 50 46
Bpu10I CC'TnA_GC
Cuts at: 2554 6150 6819 2554
Size: 3596 669 6146
Fragments arranged by size:
6146 3596 669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7457 8536 9401 9699 7457
Size: 1079 865 298 8169
Fragments arranged by size:
8169 1079 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5194 6056 9074 882
Size: 617 3695 862 3018 2219
Fragments arranged by size:
3695 3018 2219 862 617
BsaAI yAC'GTr
Cuts at: 493 1204 3180 4363 8202 493
Size: 711 1976 1183 3839 2702
Fragments arranged by size:
3839 2702 1976 1183 711
BsaBI GATnn'nnATC
Cuts at: 3789 4256 6597 7418 10405 3789
Size: 467 2341 821 2987 3795
Fragments arranged by size:
3795 2987 2341 821 467
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 3769 3832 4767
Size: 53 83 186 936 504 1732 63 935
Cuts at: 4767 6365 8218 8600 9235 275
Size: 1598 1853 382 635 1451
Fragments arranged by size:
1853 1732 1598 1451 936 935 635 504
382 186 83 63 53
BsaWI w'CCGG_w
Cuts at: 2373 3474 3636 4518 5442 5588 5755 6606 8844
Size: 1101 162 882 924 146 167 851 2238
Cuts at: 8844 9242 9516 9663 10074 2373
Size: 398 274 147 411 2710
Fragments arranged by size:
2710 2238 1101 924 882 851 411 398
274 167 162 147 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2330 2360 2579 2609 2666
Size: 30 165 30 927 30 219 30 57
Cuts at: 2666 2696 2699 2729 3506 3536 3883 3913 4331
Size: 30 3 30 777 30 347 30 418
Cuts at: 4331 4361 7574 7604 1178
Size: 30 3213 30 3985
Fragments arranged by size:
3985 3213 927 777 418 347 219 165
57 30 30 30 30 30 30 30
30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2555 2600 2671 3083 3221 3527 7082
Size: 1165 204 45 71 412 138 306 3555
Cuts at: 7082 7178 7493 7684 1186
Size: 96 315 191 3913
Fragments arranged by size:
3913 3555 1165 412 315 306 204 191
138 96 71 45
BseYI C'CCAG_C
Cuts at: 4080 4456 4746 7105 9614 10110 4080
Size: 376 290 2359 2509 496 4381
Fragments arranged by size:
4381 2509 2359 496 376 290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2127 2782 2910 3152 3214 4114 5294
Size: 573 391 655 128 242 62 900 1180
Cuts at: 5294 5780 6850 1163
Size: 486 1070 4724
Fragments arranged by size:
4724 1180 1070 900 655 573 486 391
242 128 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3292 7861 8622 9650 1540
Size: 275 186 1291 4569 761 1028 2301
Fragments arranged by size:
4569 2301 1291 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2119 2450 5619 6156 7120 8472 8557 9628 2119
Size: 331 3169 537 964 1352 85 1071 2902
Fragments arranged by size:
3169 2902 1352 1071 964 537 331 85
BsmI GAATG_Cn'
Cuts at: 3415 5575 7922 8015 3415
Size: 2160 2347 93 5811
Fragments arranged by size:
5811 2347 2160 93
BsmBI CGTCTCn'nnnn_
Cuts at: 7065 7571 7065
Size: 506 9905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2119 2450 3103 3177 3542 3634 4216
Size: 399 329 331 653 74 365 92 582
Cuts at: 4216 4457 4649 5391 5619 5920 6156 7001 7120
Size: 241 192 742 228 301 236 845 119
Cuts at: 7120 7730 8472 8557 9628 1391
Size: 610 742 85 1071 2174
Fragments arranged by size:
2174 1071 845 742 742 653 610 582
399 365 331 329 301 241 236 228
192 119 92 85 74
BspEI T'CCGG_A
Cuts at: 2373 5588 10074 2373
Size: 3215 4486 2710
Fragments arranged by size:
4486 3215 2710
BspHI T'CATG_A
Cuts at: 8300 10030 8300
Size: 1730 8681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 3149 3349 4099 4499 5099 5118 5138
Size: 1297 1008 200 750 400 600 19 20
Cuts at: 5138 5889 6078 6634 7766 844
Size: 751 189 556 1132 3489
Fragments arranged by size:
3489 1297 1132 1008 751 750 600 556
400 200 189 20 19
BsrBI CCG'CTC
Cuts at: 1394 1823 2262 2700 3375 5584 6511 8298 1394
Size: 429 439 438 675 2209 927 1787 3507
Fragments arranged by size:
3507 2209 1787 927 675 439 438 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2484 3085 3414 3678 3777 5253 5863
Size: 1183 1236 601 329 264 99 1476 610
Cuts at: 5863 6420 6471 8906 9080 65
Size: 557 51 2435 174 1396
Fragments arranged by size:
2435 1476 1396 1236 1183 610 601 557
329 264 174 99 51
BsrFI r'CCGG_y
Cuts at: 1700 1810 3051 3156 3291 4769 4920 4998 5755
Size: 110 1241 105 135 1478 151 78 757
Cuts at: 5755 9055 9242 1700
Size: 3300 187 2869
Fragments arranged by size:
3300 2869 1478 1241 757 187 151 135
110 105 78
BsrGI T'GTAC_A
Cuts at: 96 7233 7353 96
Size: 7137 120 3154
Fragments arranged by size:
7137 3154 120
BssHII G'CGCG_C
Cuts at: 1925 2022 1925
Size: 97 10314
BssSI C'ACGA_G
Cuts at: 5867 8471 9483 5867
Size: 2604 1012 6795
Fragments arranged by size:
6795 2604 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2114 3364 5130 794
Size: 1320 1250 1766 6075
Fragments arranged by size:
6075 1766 1320 1250
BstBI TT'CG_AA
Cuts at: 9215 9215
Size: 10411
BstEII G'GTnAC_C
Cuts at: 1184 4480 1184
Size: 3296 7115
BstXI CCAn_nnnn'nTGG
Cuts at: 2636 4850 2636
Size: 2214 8197
BstYI r'GATC_y
Cuts at: 1972 3477 6579 6699 7741 8108 8493 8510 9962
Size: 1505 3102 120 1042 367 385 17 1452
Cuts at: 9962 10048 10060 10406 1972
Size: 86 12 346 1977
Fragments arranged by size:
3102 1977 1505 1452 1042 385 367 346
120 86 17 12
BstZ17I GTA'TAC
Cuts at: 2204 5419 2204
Size: 3215 7196
Bsu36I CC'TnA_GG
Cuts at: 4678 4678
Size: 10411
BtgI C'CryG_G
Cuts at: 513 1065 3693 4608 6085 513
Size: 552 2628 915 1477 4839
Fragments arranged by size:
4839 2628 1477 915 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2202 2406 2436 2649 2769 2916
Size: 683 56 976 204 30 213 120 147
Cuts at: 2916 487
Size: 7982
Fragments arranged by size:
7982 976 683 213 204 147 120 56
30
BtsI GCAGTG_nn'
Cuts at: 2021 2115 2159 2386 2435 2565 2629 3135 3140
Size: 94 44 227 49 130 64 506 5
Cuts at: 3140 3386 4330 5306 6312 7927 8721 8741 2021
Size: 246 944 976 1006 1615 794 20 3691
Fragments arranged by size:
3691 1615 1006 976 944 794 506 246
227 130 94 64 49 44 20 5
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2389 2424 2995 3030 6677 6712 6826
Size: 35 1825 35 571 35 3647 35 114
Cuts at: 6826 6861 529
Size: 35 4079
Fragments arranged by size:
4079 3647 1825 571 114 35 35 35
35 35
DraI TTT'AAA
Cuts at: 7715 7715
Size: 10411
DraIII CAC_nnn'GTG
Cuts at: 5021 5021
Size: 10411
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4982 7057 9418 809
Size: 507 3666 2075 2361 1802
Fragments arranged by size:
3666 2361 2075 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 3615 4153
Size: 54 1425 50 275 18 231 1554 538
Cuts at: 4153 4467 5407 6318 6655 7858 8747 10376 8
Size: 314 940 911 337 1203 889 1629 43
Fragments arranged by size:
1629 1554 1425 1203 940 911 889 538
337 314 275 231 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7858 1537
Size: 275 6046 4090
Fragments arranged by size:
6046 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2901 3800 4705 6080 6455 7593 8341 1353
Size: 1548 899 905 1375 375 1138 748 3423
Fragments arranged by size:
3423 1548 1375 1138 905 899 748 375
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2246 2843 8998 9372 9518 387
Size: 1064 138 657 597 6155 374 146 1280
Fragments arranged by size:
6155 1280 1064 657 597 374 146 138
EcoICRI GAG'CTC
Cuts at: 6154 6154
Size: 10411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2603 3425 3554 3683 3733 6047 6064
Size: 393 971 822 129 129 50 2314 17
Cuts at: 6064 6305 7085 7131 7623 8474 9071 9858 10126
Size: 241 780 46 492 851 597 787 268
Cuts at: 10126 1239
Size: 1524
Fragments arranged by size:
2314 1524 971 851 822 787 780 597
492 393 268 241 129 129 50 46
17
EcoO109I rG'GnC_Cy
Cuts at: 3547 3630 3919 4285 4743 5061 6517 7385 7498
Size: 83 289 366 458 318 1456 868 113
Cuts at: 7498 3547
Size: 6460
Fragments arranged by size:
6460 1456 868 458 366 318 289 113
83
EcoRI G'AATT_C
Cuts at: 7720 8098 7720
Size: 378 10033
EcoRV GAT'ATC
Cuts at: 6802 6802
Size: 10411
FspI TGC'GCA
Cuts at: 2050 2441 4322 6597 8917 2050
Size: 391 1881 2275 2320 3544
Fragments arranged by size:
3544 2320 2275 1881 391
FspAI rTGC'GCAy
Cuts at: 6597 6597
Size: 10411
HaeII r_GCGC'y
Cuts at: 1678 2040 2570 2752 2869 2974 3835 4002 4087
Size: 362 530 182 117 105 861 167 85
Cuts at: 4087 4917 9558 1678
Size: 830 4641 2531
Fragments arranged by size:
4641 2531 861 830 530 362 182 167
117 105 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2598 2630 2666 2698 2765
Size: 32 135 32 1026 32 36 32 67
Cuts at: 2765 2797 3506 3538 3759 3791 3865 3897 4274
Size: 32 709 32 221 32 74 32 377
Cuts at: 4274 4306 4331 4363 4727 4759 4860 4892 6034
Size: 32 25 32 364 32 101 32 1142
Cuts at: 6034 6066 6734 6766 7149 7181 7523 7555 9052
Size: 32 668 32 383 32 342 32 1497
Cuts at: 9052 9084 9126 9158 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1497 1142 1026 709 668 383 377
364 342 221 135 101 74 67 42
36 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 3007 3085 6742 7755 8001 669
Size: 988 281 1069 78 3657 1013 246 3079
Fragments arranged by size:
3657 3079 1069 1013 988 281 246 78
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10411
HpaI GTT'AAC
Cuts at: 3085 8001 3085
Size: 4916 5495
KasI G'GCGC_C
Cuts at: 2036 3831 2036
Size: 1795 8616
KpnI G_GTAC'C
Cuts at: 3705 5228 6448 6962 7736 3705
Size: 1523 1220 514 774 6380
Fragments arranged by size:
6380 1523 1220 774 514
MfeI C'AATT_G
Cuts at: 4782 8010 4782
Size: 3228 7183
MluI A'CGCG_T
Cuts at: 10287 10287
Size: 10411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2069 2475 2942 5242 5901 9525 9709 1305
Size: 764 406 467 2300 659 3624 184 2007
Fragments arranged by size:
3624 2300 2007 764 659 467 406 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2063 3617 4155 4469 6320
Size: 54 1425 343 231 1554 538 314 1851
Cuts at: 6320 10
Size: 4101
Fragments arranged by size:
4101 1851 1554 1425 538 343 314 231
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2391 2634 2807 3944 4209 4598
Size: 648 1021 204 243 173 1137 265 389
Cuts at: 4598 5183 5240 5769 5867 5897 6129 6131 6212
Size: 585 57 529 98 30 232 2 81
Cuts at: 6212 8369 8728 8887 518
Size: 2157 359 159 2042
Fragments arranged by size:
2157 2042 1137 1021 648 585 529 389
359 265 243 232 204 173 159 98
81 57 30 2
MspA1I CmG'CkG
Cuts at: 1606 2466 2832 2847 3300 4107 4460 5085 6025
Size: 860 366 15 453 807 353 625 940
Cuts at: 6025 6823 7363 7588 7827 8504 9652 9897 1606
Size: 798 540 225 239 677 1148 245 2120
Fragments arranged by size:
2120 1148 940 860 807 798 677 625
540 453 366 353 245 239 225 15
NaeI GCC'GGC
Cuts at: 3053 4771 5000 3053
Size: 1718 229 8464
Fragments arranged by size:
8464 1718 229
NarI GG'CG_CC
Cuts at: 2037 3832 2037
Size: 1795 8616
NcoI C'CATG_G
Cuts at: 513 1065 3693 4608 513
Size: 552 2628 915 6316
Fragments arranged by size:
6316 2628 915 552
NdeI CA'TA_TG
Cuts at: 387 5770 387
Size: 5383 5028
NgoMIV G'CCGG_C
Cuts at: 3051 4769 4998 3051
Size: 1718 229 8464
Fragments arranged by size:
8464 1718 229
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10411
NotI GC'GGCC_GC
Cuts at: 7858 7858
Size: 10411
NsiI A_TGCA'T
Cuts at: 7523 7523
Size: 10411
NspI r_CATG'y
Cuts at: 1132 1229 2106 2440 4624 5902 6140 7769 9314
Size: 97 877 334 2184 1278 238 1629 1545
Cuts at: 9314 1132
Size: 2229
Fragments arranged by size:
2229 2184 1629 1545 1278 877 334 238
97
PasI CC'CwG_GG
Cuts at: 1348 3542 1348
Size: 2194 8217
PciI A'CATG_T
Cuts at: 2102 4620 9310 2102
Size: 2518 4690 3203
Fragments arranged by size:
4690 3203 2518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3711 4476 5536 1108
Size: 2603 765 1060 5983
Fragments arranged by size:
5983 2603 1060 765
PfoI T'CCnGG_A
Cuts at: 6219 7264 7506 6219
Size: 1045 242 9124
Fragments arranged by size:
9124 1045 242
PmeI GTTT'AAAC
Cuts at: 7715 7715
Size: 10411
PmlI CAC'GTG
Cuts at: 3180 4363 3180
Size: 1183 9228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2569 7674 7706 8480 8512 9993 10025 2537
Size: 32 5105 32 774 32 1481 32 2923
Fragments arranged by size:
5105 2923 1481 774 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4285 4743 5061 6517 4285
Size: 458 318 1456 8179
Fragments arranged by size:
8179 1456 458 318
PshAI GACnn'nnGTC
Cuts at: 1613 4260 4376 6345 1613
Size: 2647 116 1969 5679
Fragments arranged by size:
5679 2647 1969 116
PsiI TTA'TAA
Cuts at: 7981 7981
Size: 10411
PspOMI G'GGCC_C
Cuts at: 3630 3630
Size: 10411
PspXI vC'TCGA_Gb
Cuts at: 3601 3601
Size: 10411
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7268 7300 7268
Size: 32 10379
PstI C_TGCA'G
Cuts at: 830 4096 4953 7633 7763 830
Size: 3266 857 2680 130 3478
Fragments arranged by size:
3478 3266 2680 857 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10411
PvuII CAG'CTG
Cuts at: 3300 4460 5085 6025 7363 7588 3300
Size: 1160 625 940 1338 225 6123
Fragments arranged by size:
6123 1338 1160 940 625 225
RsrII CG'GwC_CG
Cuts at: 5586 5586
Size: 10411
SacI G_AGCT'C
Cuts at: 6156 6156
Size: 10411
SalI G'TCGA_C
Cuts at: 1936 7753 1936
Size: 5817 4594
SanDI GG'GwC_CC
Cuts at: 4743 4743
Size: 10411
SapI GCTCTTCn'nnn_
Cuts at: 6080 7593 6080
Size: 1513 8898
SbfI CC_TGCA'GG
Cuts at: 4096 7763 4096
Size: 3667 6744
ScaI AGT'ACT
Cuts at: 4560 6396 8659 10371 4560
Size: 1836 2263 1712 4600
Fragments arranged by size:
4600 2263 1836 1712
SfcI C'TryA_G
Cuts at: 826 1045 2467 4092 4949 5041 5372 7285 7629
Size: 219 1422 1625 857 92 331 1913 344
Cuts at: 7629 7759 8894 9575 9766 826
Size: 130 1135 681 191 1471
Fragments arranged by size:
1913 1625 1471 1422 1135 857 681 344
331 219 191 130 92
SfoI GGC'GCC
Cuts at: 2038 3833 2038
Size: 1795 8616
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10411
SgrAI Cr'CCGG_yG
Cuts at: 1700 5755 1700
Size: 4055 6356
SmaI CCC'GGG
Cuts at: 1872 2327 3674 4352 5513 7738 1872
Size: 455 1347 678 1161 2225 4545
Fragments arranged by size:
4545 2225 1347 1161 678 455
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3601 5947 7472 8515 9416 9678
Size: 197 926 1658 2346 1525 1043 901 262
Cuts at: 9678 820
Size: 1553
Fragments arranged by size:
2346 1658 1553 1525 1043 926 901 262
197
SnaBI TAC'GTA
Cuts at: 493 8202 493
Size: 7709 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10411
SphI G_CATG'C
Cuts at: 1132 7769 1132
Size: 6637 3774
SspI AAT'ATT
Cuts at: 5 52 8335 5
Size: 47 8283 2081
Fragments arranged by size:
8283 2081 47
StuI AGG'CCT
Cuts at: 1517 3746 5889 1517
Size: 2229 2143 6039
Fragments arranged by size:
6039 2229 2143
StyI C'CwwG_G
Cuts at: 513 1065 2943 3693 4608 5632 6971 7404 7850
Size: 552 1878 750 915 1024 1339 433 446
Cuts at: 7850 513
Size: 3074
Fragments arranged by size:
3074 1878 1339 1024 915 750 552 446
433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2257 3602 3835 4860 5781 6304 6577
Size: 1255 677 1345 233 1025 921 523 273
Cuts at: 6577 6884 8438 8455 8608 8793 9238 325
Size: 307 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1345 1255 1025 921 677 523
445 307 273 233 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3381 3840 3963
Size: 274 80 33 51 1443 1404 459 123
Cuts at: 3963 4365 4494 4558 5289 5547 6330 6394 7233
Size: 402 129 64 731 258 783 64 839
Cuts at: 7233 7353 8657 10369 96
Size: 120 1304 1712 138
Fragments arranged by size:
1712 1443 1404 1304 839 783 731 459
402 274 258 138 129 123 120 80
64 64 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2579 2611 2699 2731 3469 3501 4413 4445 5308
Size: 32 88 32 738 32 912 32 863
Cuts at: 5308 5340 5640 5672 6031 6063 6601 6633 7232
Size: 32 300 32 359 32 538 32 599
Cuts at: 7232 7264 10371 10403 2579
Size: 32 3107 32 2587
Fragments arranged by size:
3107 2587 912 863 738 599 538 359
300 88 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6724 1316
Size: 5408 5003
XbaI T'CTAG_A
Cuts at: 7747 7747
Size: 10411
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2600 6298 7553 7668 2600
Size: 3698 1255 115 5343
Fragments arranged by size:
5343 3698 1255 115
XhoI C'TCGA_G
Cuts at: 1943 3601 5947 1943
Size: 1658 2346 6407
Fragments arranged by size:
6407 2346 1658
XmaI C'CCGG_G
Cuts at: 1870 2325 3672 4350 5511 7736 1870
Size: 455 1347 678 1161 2225 4545
Fragments arranged by size:
4545 2225 1347 1161 678 455
XmnI GAAnn'nnTTC
Cuts at: 4032 5150 7158 8540 4032
Size: 1118 2008 1382 5903
Fragments arranged by size:
5903 2008 1382 1118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8219 276
Size: 53 83 186 936 6685 2468
Fragments arranged by size:
6685 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BsiWI EcoNI FalI FseI NruI
PacI SacII SexAI SfiI SrfI SwaI