Restriction map for
pFN21AA0815
(Circular) (Six-base) MAPSORT of: pFN21AA0815.seq Check: 2146 from: 1 to: 8392
pFN21AA0815 8392 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2830 3617 3797 4496 5135 5219 5393 2830
Size: 787 180 699 639 84 174 5829
Fragments arranged by size:
5829 787 699 639 180 174 84
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3159 4674 6202 278
Size: 53 83 186 936 1623 1515 1528 2468
Fragments arranged by size:
2468 1623 1528 1515 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 5735 1937
Size: 871 2927 4594
Fragments arranged by size:
4594 2927 871
Acc65I G'GTAC_C
Cuts at: 5713 5713
Size: 8392
AclI AA'CG_TT
Cuts at: 1563 6519 6892 1563
Size: 4956 373 3063
Fragments arranged by size:
4956 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 6455 7839 8107 1632
Size: 4823 1384 268 1917
Fragments arranged by size:
4823 1917 1384 268
AfeI AGC'GCT
Cuts at: 3731 4318 4823 3731
Size: 587 505 7300
Fragments arranged by size:
7300 587 505
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8195
AflIII A'CryG_T
Cuts at: 1203 2756 4134 5079 5363 7291 8268 1203
Size: 1553 1378 945 284 1928 977 1327
Fragments arranged by size:
1928 1553 1378 1327 977 945 284
AgeI A'CCGG_T
Cuts at: 2211 5184 7223 2211
Size: 2973 2039 3380
Fragments arranged by size:
3380 2973 2039
AhdI GACnn_n'nnGTC
Cuts at: 7121 7121
Size: 8392
AleI CACnn'nnGTG
Cuts at: 1166 2196 2337 2736 5078 5082 1166
Size: 1030 141 399 2342 4 4476
Fragments arranged by size:
4476 2342 1030 399 141 4
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2471 3805 1184
Size: 1287 1334 5771
Fragments arranged by size:
5771 1334 1287
AlwNI CAG_nnn'CTG
Cuts at: 2286 4125 4566 6135 7707 2286
Size: 1839 441 1569 1572 2971
Fragments arranged by size:
2971 1839 1572 1569 441
ApaI G_GGCC'C
Cuts at: 2748 3937 4382 4422 4640 5481 5571 5592 2748
Size: 1189 445 40 218 841 90 21 5548
Fragments arranged by size:
5548 1189 841 445 218 90 40 21
ApaLI G'TGCA_C
Cuts at: 2752 2845 6449 7605 2752
Size: 93 3604 1156 3539
Fragments arranged by size:
3604 3539 1156 93
ApoI r'AATT_y
Cuts at: 1493 5701 5927 6079 8188 1493
Size: 4208 226 152 2109 1697
Fragments arranged by size:
4208 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6946 160
Size: 872 5914 1606
Fragments arranged by size:
5914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8392
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2381 3317 3448 3910 4010 4235
Size: 73 73 365 936 131 462 100 225
Cuts at: 4235 4665 4922 5021 5140 5603 5717 1870
Size: 430 257 99 119 463 114 4545
Fragments arranged by size:
4545 936 463 462 430 365 257 225
131 119 114 100 99 73 73
BamHI G'GATC_C
Cuts at: 5722 6089 5722
Size: 367 8025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2463 2642 3543 4508
Size: 325 217 627 256 420 179 901 965
Cuts at: 4508 5123 5381 5713 7168 618
Size: 615 258 332 1455 1842
Fragments arranged by size:
1842 1455 965 901 627 615 420 332
325 258 256 217 179
BanII G_rGCy'C
Cuts at: 1391 2055 2748 3336 3585 3917 3937 4382 4422
Size: 664 693 588 249 332 20 445 40
Cuts at: 4422 4640 4784 4976 5481 5571 5592 5630 5711
Size: 218 144 192 505 90 21 38 81
Cuts at: 5711 1391
Size: 4072
Fragments arranged by size:
4072 693 664 588 505 445 332 249
218 192 144 90 81 40 38 21
20
BbeI G_GCGC'C
Cuts at: 2646 2646
Size: 8392
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3996 928
Size: 971 2097 5324
Fragments arranged by size:
5324 2097 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2788 2798 2822 2832 6583 6617 1903
Size: 34 851 10 24 10 3751 34 3678
Fragments arranged by size:
3751 3678 851 34 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 6284 7494 6284
Size: 1210 7182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2136 2505
Size: 107 122 71 631 605 337 127 369
Cuts at: 2505 2952 3294 3303 3321 3489 3798 4107 4146
Size: 447 342 9 18 168 309 309 39
Cuts at: 4146 4497 4532 4536 4626 4635 4755 4794 4994
Size: 351 35 4 90 9 120 39 200
Cuts at: 4994 5061 5394 5596 7003 136
Size: 67 333 202 1407 1525
Fragments arranged by size:
1525 1407 631 605 447 369 351 342
337 333 309 309 202 200 168 127
122 120 107 90 71 67 39 39
35 18 9 9 4
BglII A'GATC_T
Cuts at: 4041 8387 4041
Size: 4346 4046
BlpI GC'TnA_GC
Cuts at: 5629 5809 5629
Size: 180 8212
Bme1580I G_kGCm'C
Cuts at: 1790 2084 2581 2748 2756 2849 3884 3937 4142
Size: 294 497 167 8 93 1035 53 205
Cuts at: 4142 4200 4234 4281 4382 4422 4511 4640 4819
Size: 58 34 47 101 40 89 129 179
Cuts at: 4819 5384 5481 5571 5592 5639 6453 7609 1790
Size: 565 97 90 21 47 814 1156 2573
Fragments arranged by size:
2573 1156 1035 814 565 497 294 205
179 167 129 101 97 93 90 89
58 53 47 47 40 34 21 8
BmgBI CAC'GTC
Cuts at: 1318 4135 4471 4858 1318
Size: 2817 336 387 4852
Fragments arranged by size:
4852 2817 387 336
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3339 3788
Size: 416 53 83 399 24 1149 773 449
Cuts at: 3788 3993 4284 4291 4773 4872 5022 6123 7081
Size: 205 291 7 482 99 150 1101 958
Cuts at: 7081 442
Size: 1753
Fragments arranged by size:
1753 1149 1101 958 773 482 449 416
399 291 205 150 99 83 53 24
7
BmtI G_CTAG'C
Cuts at: 1056 2519 1056
Size: 1463 6929
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 8392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2122 2378 2926 3071 3104 4522 5357 5635
Size: 883 256 548 145 33 1418 835 278
Cuts at: 5635 7052 1239
Size: 1417 2579
Fragments arranged by size:
2579 1418 1417 883 835 548 278 256
145 33
Bpu10I CC'TnA_GC
Cuts at: 5434 5617 5434
Size: 183 8209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6517 7382 7680 6517
Size: 865 298 7229
Fragments arranged by size:
7229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4076 7055 882
Size: 617 2577 2979 2219
Fragments arranged by size:
2979 2577 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2503 4375 5080 5364 5574 6183 493
Size: 711 1299 1872 705 284 210 609 2702
Fragments arranged by size:
2702 1872 1299 711 705 609 284 210
BsaBI GATnn'nnATC
Cuts at: 2678 8386 2678
Size: 5708 2684
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2643 3156 4671
Size: 53 83 186 936 479 631 513 1515
Cuts at: 4671 5154 6199 6581 7216 275
Size: 483 1045 382 635 1451
Fragments arranged by size:
1515 1451 1045 936 635 631 513 483
479 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2211 2664 3358 4085 4214 5184 6825 7223 7497
Size: 453 694 727 129 970 1641 398 274
Cuts at: 7497 7644 8055 2211
Size: 147 411 2548
Fragments arranged by size:
2548 1641 970 727 694 453 411 398
274 147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3340 3370 3638 3668 4958
Size: 30 165 30 1937 30 268 30 1290
Cuts at: 4958 4988 5198 5228 1178
Size: 30 210 30 4342
Fragments arranged by size:
4342 1937 1290 268 210 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3068 3659 1186
Size: 1882 591 5919
Fragments arranged by size:
5919 1882 591
BseYI C'CCAG_C
Cuts at: 2638 2741 3751 4020 4027 4048 4141 4160 4207
Size: 103 1010 269 7 21 93 19 47
Cuts at: 4207 4750 4818 5480 7595 8091 2638
Size: 543 68 662 2115 496 2939
Fragments arranged by size:
2939 2115 1010 662 543 496 269 103
93 68 47 21 19 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2838 2953 3005 3680 4468 4558 5056
Size: 573 1102 115 52 675 788 90 498
Cuts at: 5056 5470 5477 1163
Size: 414 7 4078
Fragments arranged by size:
4078 1102 788 675 573 498 414 115
90 52 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4497 4536 5842 6603 7631
Size: 275 186 1591 905 39 1306 761 1028
Cuts at: 7631 1540
Size: 2301
Fragments arranged by size:
2301 1591 1306 1028 905 761 275 186
39
BsiHKAI G_wGCw'C
Cuts at: 2756 2849 2964 2999 3120 3917 4181 5208 5345
Size: 93 115 35 121 797 264 1027 137
Cuts at: 5345 5427 6453 6538 7609 2756
Size: 82 1026 85 1071 3539
Fragments arranged by size:
3539 1071 1027 1026 797 264 137 121
115 93 85 82 35
BsmI GAATG_Cn'
Cuts at: 2186 5903 5996 2186
Size: 3717 93 4582
Fragments arranged by size:
4582 3717 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2084 2581 2748 2756 2849 2964
Size: 399 265 29 497 167 8 93 115
Cuts at: 2964 2999 3120 3336 3585 3884 3917 3937 4142
Size: 35 121 216 249 299 33 20 205
Cuts at: 4142 4181 4200 4234 4281 4382 4422 4511 4640
Size: 39 19 34 47 101 40 89 129
Cuts at: 4640 4784 4819 4976 5208 5345 5384 5427 5481
Size: 144 35 157 232 137 39 43 54
Cuts at: 5481 5571 5592 5630 5639 5711 6453 6538 7609
Size: 90 21 38 9 72 742 85 1071
Cuts at: 7609 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 497 399 299 265 249
232 216 205 167 157 144 137 129
121 115 101 93 90 89 85 72
54 47 43 40 39 39 38 35
35 34 33 29 21 20 19 9
8
BspEI T'CCGG_A
Cuts at: 3358 8055 3358
Size: 4697 3695
BspHI T'CATG_A
Cuts at: 6281 8011 6281
Size: 1730 6662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 2804 2830 3293 3422 3617 3797 4496
Size: 1450 510 26 463 129 195 180 699
Cuts at: 4496 5135 5219 5393 5747 844
Size: 639 84 174 354 3489
Fragments arranged by size:
3489 1450 699 639 510 463 354 195
180 174 129 84 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 5673 6279 1394
Size: 429 1999 1851 606 3507
Fragments arranged by size:
3507 1999 1851 606 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2323 3108 3349 6887 7061 65
Size: 1183 1075 785 241 3538 174 1396
Fragments arranged by size:
3538 1396 1183 1075 785 241 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 2153 2211 2376 2652 2885 2892
Size: 110 325 18 58 165 276 233 7
Cuts at: 2892 2981 3008 3015 3540 3963 4244 4496 4535
Size: 89 27 7 525 423 281 252 39
Cuts at: 4535 4686 4754 5120 5150 5184 5427 7036 7223
Size: 151 68 366 30 34 243 1609 187
Cuts at: 7223 1700
Size: 2869
Fragments arranged by size:
2869 1609 525 423 366 325 281 276
252 243 233 187 165 151 110 89
68 58 39 34 30 27 18 7
7
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8392
BssHII G'CGCG_C
Cuts at: 1925 2069 1925
Size: 144 8248
BssSI C'ACGA_G
Cuts at: 3023 6452 7464 3023
Size: 3429 1012 3951
Fragments arranged by size:
3951 3429 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2286 3519 4125 4515 4566 794
Size: 1492 1233 606 390 51 4620
Fragments arranged by size:
4620 1492 1233 606 390 51
BstBI TT'CG_AA
Cuts at: 7196 7196
Size: 8392
BstEII G'GTnAC_C
Cuts at: 1184 2156 1184
Size: 972 7420
BstXI CCAn_nnnn'nTGG
Cuts at: 2198 4149 4758 4862 5084 2198
Size: 1951 609 104 222 5506
Fragments arranged by size:
5506 1951 609 222 104
BstYI r'GATC_y
Cuts at: 1972 4041 5722 6089 6474 6491 7943 8029 8041
Size: 2069 1681 367 385 17 1452 86 12
Cuts at: 8041 8387 1972
Size: 346 1977
Fragments arranged by size:
2069 1977 1681 1452 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2023 2581 2719 2764 2947 3617
Size: 552 939 19 558 138 45 183 670
Cuts at: 3617 3943 4324 4382 4989 5376 513
Size: 326 381 58 607 387 3529
Fragments arranged by size:
3529 939 670 607 558 552 387 381
326 183 138 58 45 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2348 487
Size: 683 56 1122 6531
Fragments arranged by size:
6531 1122 683 56
BtsI GCAGTG_nn'
Cuts at: 2663 2965 3965 4198 4219 4403 4567 5287 5546
Size: 302 1000 233 21 184 164 720 259
Cuts at: 5546 5639 5908 6702 6722 2663
Size: 93 269 794 20 4333
Fragments arranged by size:
4333 1000 794 720 302 269 259 233
184 164 93 21 20
ClaI AT'CG_AT
Cuts at: 1554 2802 1554
Size: 1248 7144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8357
DraI TTT'AAA
Cuts at: 5696 5696
Size: 8392
DraIII CAC_nnn'GTG
Cuts at: 5551 5551
Size: 8392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7399 809
Size: 507 6083 1802
Fragments arranged by size:
6083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2292 2650
Size: 54 1425 50 275 18 201 261 358
Cuts at: 2650 2979 3238 3589 3871 4321 4494 4533 4627
Size: 329 259 351 282 450 173 39 94
Cuts at: 4627 4986 5410 5839 6728 8357 8
Size: 359 424 429 889 1629 43
Fragments arranged by size:
1629 1425 889 450 429 424 359 358
351 329 282 275 261 259 201 173
94 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 4494 4533 5839 1537
Size: 275 1777 905 39 1306 4090
Fragments arranged by size:
4090 1777 1306 905 275 39
EarI CTCTTCn'nnn_
Cuts at: 1353 3146 3168 6322 1353
Size: 1793 22 3154 3423
Fragments arranged by size:
3423 3154 1793 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4908 6979 7353 7499 387
Size: 1064 138 3319 2071 374 146 1280
Fragments arranged by size:
3319 2071 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3915 3915
Size: 8392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2378 2926 3071 3104 4522 5357
Size: 393 490 256 548 145 33 1418 835
Cuts at: 5357 5635 6455 7052 7839 8107 1239
Size: 278 820 597 787 268 1524
Fragments arranged by size:
1524 1418 835 820 787 597 548 490
393 278 268 256 145 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2129
Size: 8392
EcoO109I rG'GnC_Cy
Cuts at: 2062 2283 2479 2604 2745 2793 3282 3411 3432
Size: 221 196 125 141 48 489 129 21
Cuts at: 3432 3561 3933 3934 4286 4479 4636 4637 4714
Size: 129 372 1 352 193 157 1 77
Cuts at: 4714 4866 4995 5230 5567 5589 2062
Size: 152 129 235 337 22 4865
Fragments arranged by size:
4865 489 372 352 337 235 221 196
193 157 152 141 129 129 129 125
77 48 22 21 1 1
EcoRI G'AATT_C
Cuts at: 5701 6079 5701
Size: 378 8014
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 3733 4197 2275
Size: 1458 464 6470
Fragments arranged by size:
6470 1458 464
FspI TGC'GCA
Cuts at: 6898 6898
Size: 8392
HaeII r_GCGC'y
Cuts at: 1678 2154 2646 3733 4320 4825 5317 5434 7539
Size: 476 492 1087 587 505 492 117 2105
Cuts at: 7539 1678
Size: 2531
Fragments arranged by size:
2531 2105 1087 587 505 492 492 476
117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3340 3372 5139 5171 5597
Size: 32 135 32 1768 32 1767 32 426
Cuts at: 5597 5629 7033 7065 7107 7139 1373
Size: 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1768 1767 1404 426 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2809 5736 5982 669
Size: 988 281 871 2927 246 3079
Fragments arranged by size:
3079 2927 988 871 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8392
HpaI GTT'AAC
Cuts at: 5982 5982
Size: 8392
KasI G'GCGC_C
Cuts at: 2642 2642
Size: 8392
KpnI G_GTAC'C
Cuts at: 5717 5717
Size: 8392
MfeI C'AATT_G
Cuts at: 5991 5991
Size: 8392
MluI A'CGCG_T
Cuts at: 8268 8268
Size: 8392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7506 7690 1305
Size: 6201 184 2007
Fragments arranged by size:
6201 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 3240 3873 4323 5412
Size: 54 1425 343 462 946 633 450 1089
Cuts at: 5412 10
Size: 2990
Fragments arranged by size:
2990 1425 1089 946 633 462 450 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 2337 2736 3113 5078 5082 6350
Size: 648 1030 141 399 377 1965 4 1268
Cuts at: 6350 6709 6868 518
Size: 359 159 2042
Fragments arranged by size:
2042 1965 1268 1030 648 399 377 359
159 141 4
MspA1I CmG'CkG
Cuts at: 1606 2025 2451 2490 2522 2691 2766 3396 3441
Size: 419 426 39 32 169 75 630 45
Cuts at: 3441 3699 4020 4071 4125 4164 4227 4347 4384
Size: 258 321 51 54 39 63 120 37
Cuts at: 4384 4435 4476 4625 4644 4695 4740 4830 4844
Size: 51 41 149 19 51 45 90 14
Cuts at: 4844 4875 4951 5004 5160 5256 5391 5484 5633
Size: 31 76 53 156 96 135 93 149
Cuts at: 5633 5808 6485 7633 7878 1606
Size: 175 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 426 419 321 258
245 175 169 156 149 149 135 120
96 93 90 76 75 63 54 53
51 51 51 45 45 41 39 39
37 32 31 19 14
NaeI GCC'GGC
Cuts at: 2137 2378 2654 2887 2983 3010 4498 4537 4688
Size: 241 276 233 96 27 1488 39 151
Cuts at: 4688 4756 5152 2137
Size: 68 396 5377
Fragments arranged by size:
5377 1488 396 276 241 233 151 96
68 39 27
NarI GG'CG_CC
Cuts at: 2643 2643
Size: 8392
NcoI C'CATG_G
Cuts at: 513 1065 2004 2581 2719 513
Size: 552 939 577 138 6186
Fragments arranged by size:
6186 939 577 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8392
NgoMIV G'CCGG_C
Cuts at: 2135 2376 2652 2885 2981 3008 4496 4535 4686
Size: 241 276 233 96 27 1488 39 151
Cuts at: 4686 4754 5150 2135
Size: 68 396 5377
Fragments arranged by size:
5377 1488 396 276 241 233 151 96
68 39 27
NheI G'CTAG_C
Cuts at: 1052 2515 1052
Size: 1463 6929
NotI GC'GGCC_GC
Cuts at: 5839 5839
Size: 8392
NspI r_CATG'y
Cuts at: 1132 1229 2458 2760 3786 4056 4526 5750 7295
Size: 97 1229 302 1026 270 470 1224 1545
Cuts at: 7295 1132
Size: 2229
Fragments arranged by size:
2229 1545 1229 1224 1026 470 302 270
97
PasI CC'CwG_GG
Cuts at: 1348 2311 2599 3578 4405 5309 5623 1348
Size: 963 288 979 827 904 314 4117
Fragments arranged by size:
4117 979 963 904 827 314 288
PciI A'CATG_T
Cuts at: 2756 7291 2756
Size: 4535 3857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8392
PfoI T'CCnGG_A
Cuts at: 2394 3120 3144 5666 2394
Size: 726 24 2522 5120
Fragments arranged by size:
5120 2522 726 24
PmeI GTTT'AAAC
Cuts at: 5696 5696
Size: 8392
PmlI CAC'GTG
Cuts at: 2503 5080 5364 5574 2503
Size: 2577 284 210 5321
Fragments arranged by size:
5321 2577 284 210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3186 3218 6461 6493 7974 8006 3186
Size: 32 3243 32 1481 32 3572
Fragments arranged by size:
3572 3243 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 2604 2793 3282 3411 4286 2283
Size: 321 189 489 129 875 6389
Fragments arranged by size:
6389 875 489 321 189 129
PshAI GACnn'nnGTC
Cuts at: 1613 4773 1613
Size: 3160 5232
PsiI TTA'TAA
Cuts at: 5962 5962
Size: 8392
PspOMI G'GGCC_C
Cuts at: 2744 3933 4378 4418 4636 5477 5567 5588 2744
Size: 1189 445 40 218 841 90 21 5548
Fragments arranged by size:
5548 1189 841 445 218 90 40 21
PspXI vC'TCGA_Gb
Cuts at: 3910 3910
Size: 8392
PstI C_TGCA'G
Cuts at: 830 2729 3044 3251 3272 3383 3395 4226 4298
Size: 1899 315 207 21 111 12 831 72
Cuts at: 4298 4316 4829 5222 5744 830
Size: 18 513 393 522 3478
Fragments arranged by size:
3478 1899 831 522 513 393 315 207
111 72 21 18 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8392
PvuII CAG'CTG
Cuts at: 2451 2691 3396 3699 4164 4227 4435 4830 4951
Size: 240 705 303 465 63 208 395 121
Cuts at: 4951 5256 5484 2451
Size: 305 228 5359
Fragments arranged by size:
5359 705 465 395 305 303 240 228
208 121 63
SacI G_AGCT'C
Cuts at: 3917 3917
Size: 8392
SacII CC_GC'GG
Cuts at: 2026 2767 4385 2026
Size: 741 1618 6033
Fragments arranged by size:
6033 1618 741
SalI G'TCGA_C
Cuts at: 1936 2807 5734 1936
Size: 871 2927 4594
Fragments arranged by size:
4594 2927 871
SbfI CC_TGCA'GG
Cuts at: 5744 5744
Size: 8392
ScaI AGT'ACT
Cuts at: 6640 8352 6640
Size: 1712 6680
SfcI C'TryA_G
Cuts at: 826 1045 2725 3040 3247 3268 3379 3391 4222
Size: 219 1680 315 207 21 111 12 831
Cuts at: 4222 4294 4312 4825 5218 5740 6875 7556 7747
Size: 72 18 513 393 522 1135 681 191
Cuts at: 7747 826
Size: 1471
Fragments arranged by size:
1680 1471 1135 831 681 522 513 393
315 219 207 191 111 72 21 18
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 4635 4994 5061 4635
Size: 359 67 7966
Fragments arranged by size:
7966 359 67
SfoI GGC'GCC
Cuts at: 2644 2644
Size: 8392
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8392
SgrAI Cr'CCGG_yG
Cuts at: 1700 2153 2211 5427 1700
Size: 453 58 3216 4665
Fragments arranged by size:
4665 3216 453 58
SmaI CCC'GGG
Cuts at: 1872 2018 2383 3450 4237 4667 4924 5142 5719
Size: 146 365 1067 787 430 257 218 577
Cuts at: 5719 1872
Size: 4545
Fragments arranged by size:
4545 1067 787 577 430 365 257 218
146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3910 6496 7397 7659 820
Size: 197 926 1967 2586 901 262 1553
Fragments arranged by size:
2586 1967 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6183 493
Size: 5690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8392
SphI G_CATG'C
Cuts at: 1132 2458 3786 4056 4526 5750 1132
Size: 1326 1328 270 470 1224 3774
Fragments arranged by size:
3774 1328 1326 1224 470 270
SrfI GCCC'GGGC
Cuts at: 4924 4924
Size: 8392
SspI AAT'ATT
Cuts at: 5 52 6316 5
Size: 47 6264 2081
Fragments arranged by size:
6264 2081 47
StuI AGG'CCT
Cuts at: 1517 2534 4811 5417 1517
Size: 1017 2277 606 4492
Fragments arranged by size:
4492 2277 1017 606
StyI C'CwwG_G
Cuts at: 513 1065 2004 2047 2581 2719 3486 5831 513
Size: 552 939 43 534 138 767 2345 3074
Fragments arranged by size:
3074 2345 939 767 552 534 138 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2612 4221 4947 5076 5090 6419 6436
Size: 1255 1032 1609 726 129 14 1329 17
Cuts at: 6436 6589 6774 7219 325
Size: 153 185 445 1498
Fragments arranged by size:
1609 1498 1329 1255 1032 726 445 185
153 129 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6638 8350 96
Size: 274 80 33 51 1443 4661 1712 138
Fragments arranged by size:
4661 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3659 3670 3691 5198 5230 8352 8384 3638
Size: 21 11 21 1507 32 3122 32 3646
Fragments arranged by size:
3646 3122 1507 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1316 3155 3422 4469 5528 1316
Size: 1839 267 1047 1059 4180
Fragments arranged by size:
4180 1839 1059 1047 267
XbaI T'CTAG_A
Cuts at: 5728 5728
Size: 8392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2738 4412 4448 4859 5186 5449 2579
Size: 159 1674 36 411 327 263 5522
Fragments arranged by size:
5522 1674 411 327 263 159 36
XhoI C'TCGA_G
Cuts at: 1943 3910 1943
Size: 1967 6425
XmaI C'CCGG_G
Cuts at: 1870 2016 2381 3448 4235 4665 4922 5140 5717
Size: 146 365 1067 787 430 257 218 577
Cuts at: 5717 1870
Size: 4545
Fragments arranged by size:
4545 1067 787 577 430 365 257 218
146
XmnI GAAnn'nnTTC
Cuts at: 6521 6521
Size: 8392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3157 4672 6200 276
Size: 53 83 186 936 1623 1515 1528 2468
Fragments arranged by size:
2468 1623 1528 1515 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI
PsrI RsrII SanDI SapI SexAI SwaI