Restriction map for
pFN21AA0808
(Circular) (Six-base) MAPSORT of: pFN21AA0808.seq Check: 6441 from: 1 to: 6283
pFN21AA0808 6283 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4093 278
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3626 1937
Size: 1689 4594
Acc65I G'GTAC_C
Cuts at: 3604 3604
Size: 6283
AclI AA'CG_TT
Cuts at: 1563 4410 4783 1563
Size: 2847 373 3063
Fragments arranged by size:
3063 2847 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2990 4346 5730 5998 1632
Size: 1358 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1358 1356 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6086
AflIII A'CryG_T
Cuts at: 1203 2121 5182 6159 1203
Size: 918 3061 977 1327
Fragments arranged by size:
3061 1327 977 918
AgeI A'CCGG_T
Cuts at: 3514 5114 3514
Size: 1600 4683
AhdI GACnn_n'nnGTC
Cuts at: 5012 5012
Size: 6283
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2586 2618 2586
Size: 32 6251
AlwNI CAG_nnn'CTG
Cuts at: 3224 4026 5598 3224
Size: 802 1572 3909
Fragments arranged by size:
3909 1572 802
ApaI G_GGCC'C
Cuts at: 3090 3090
Size: 6283
ApaLI G'TGCA_C
Cuts at: 2255 4340 5496 2255
Size: 2085 1156 3042
Fragments arranged by size:
3042 2085 1156
ApoI r'AATT_y
Cuts at: 1493 3592 3818 3970 6079 1493
Size: 2099 226 152 2109 1697
Fragments arranged by size:
2109 2099 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4837 160
Size: 872 3805 1606
Fragments arranged by size:
3805 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
AvaI C'yCGr_G
Cuts at: 1870 1943 3608 1870
Size: 73 1665 4545
Fragments arranged by size:
4545 1665 73
BamHI G'GATC_C
Cuts at: 2773 3613 3980 2773
Size: 840 367 5076
Fragments arranged by size:
5076 840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3604 5059 618
Size: 325 217 627 1817 1455 1842
Fragments arranged by size:
1842 1817 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2787 3090 3602 1391
Size: 1396 303 512 4072
Fragments arranged by size:
4072 1396 512 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 3041 928
Size: 971 309 833 4170
Fragments arranged by size:
4170 971 833 309
BbvCI CC'TCA_GC
Cuts at: 3051 3051
Size: 6283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4474 4508 1903
Size: 34 2537 34 3678
Fragments arranged by size:
3678 2537 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3013 4175 5385 3013
Size: 1162 1210 3911
Fragments arranged by size:
3911 1210 1162
BclI T'GATC_A
Cuts at: 1548 3057 1548
Size: 1509 4774
BfrBI ATG'CAT
Cuts at: 3169 3169
Size: 6283
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3104 4894 136
Size: 107 122 71 631 605 1432 1790 1525
Fragments arranged by size:
1790 1525 1432 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6278 6278
Size: 6283
BlpI GC'TnA_GC
Cuts at: 3700 3700
Size: 6283
Bme1580I G_kGCm'C
Cuts at: 1790 2259 3090 4344 5500 1790
Size: 469 831 1254 1156 2573
Fragments arranged by size:
2573 1254 1156 831 469
BmgBI CAC'GTC
Cuts at: 1318 3031 1318
Size: 1713 4570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4014 4972 442
Size: 416 53 83 399 24 2597 958 1753
Fragments arranged by size:
2597 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2013 3251 4943 1239
Size: 774 1238 1692 2579
Fragments arranged by size:
2579 1692 1238 774
Bpu10I CC'TnA_GC
Cuts at: 3051 3051
Size: 6283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4408 5273 5571 4408
Size: 865 298 5120
Fragments arranged by size:
5120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2473 4946 882
Size: 617 974 2473 2219
Fragments arranged by size:
2473 2219 974 617
BsaAI yAC'GTr
Cuts at: 493 1204 4074 493
Size: 711 2870 2702
Fragments arranged by size:
2870 2702 711
BsaBI GATnn'nnATC
Cuts at: 6277 6277
Size: 6283
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4090 4472 5107 275
Size: 53 83 186 936 2557 382 635 1451
Fragments arranged by size:
2557 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3514 4716 5114 5388 5535 5946 3514
Size: 1202 398 274 147 411 3851
Fragments arranged by size:
3851 1202 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6058
Fragments arranged by size:
6058 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2217 3039 3246 3249 1186
Size: 1031 822 207 3 4220
Fragments arranged by size:
4220 1031 822 207 3
BseYI C'CCAG_C
Cuts at: 2034 2374 3089 5486 5982 2034
Size: 340 715 2397 496 2335
Fragments arranged by size:
2397 2335 715 496 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3435 3514 1163
Size: 573 1699 79 3932
Fragments arranged by size:
3932 1699 573 79
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3733 4494 5522 1540
Size: 275 186 1732 761 1028 2301
Fragments arranged by size:
2301 1732 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2572 4344 4429 5500 2259
Size: 313 1772 85 1071 3042
Fragments arranged by size:
3042 1772 1071 313 85
BsmI GAATG_Cn'
Cuts at: 3794 3887 3794
Size: 93 6190
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2572 2787 3090 3602 4344 4429
Size: 399 469 313 215 303 512 742 85
Cuts at: 4429 5500 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 512 469 399 313 303
215 85
BspEI T'CCGG_A
Cuts at: 5946 5946
Size: 6283
BspHI T'CATG_A
Cuts at: 4172 5902 4172
Size: 1730 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3349 3638 844
Size: 2505 289 3489
Fragments arranged by size:
3489 2505 289
BsrBI CCG'CTC
Cuts at: 1394 1823 3319 4170 1394
Size: 429 1496 851 3507
Fragments arranged by size:
3507 1496 851 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2605 3477 4778 4952 65
Size: 1183 1357 872 1301 174 1396
Fragments arranged by size:
1396 1357 1301 1183 872 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3514 4927 5114 1700
Size: 110 1704 1413 187 2869
Fragments arranged by size:
2869 1704 1413 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6283
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6283
BssSI C'ACGA_G
Cuts at: 4343 5355 4343
Size: 1012 5271
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6283
BstBI TT'CG_AA
Cuts at: 3065 5087 3065
Size: 2022 4261
BstEII G'GTnAC_C
Cuts at: 1184 2279 1184
Size: 1095 5188
BstXI CCAn_nnnn'nTGG
Cuts at: 2374 2374
Size: 6283
BstYI r'GATC_y
Cuts at: 1972 2773 3613 3980 4365 4382 5834 5920 5932
Size: 801 840 367 385 17 1452 86 12
Cuts at: 5932 6278 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 840 801 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3082 513
Size: 552 939 1078 3714
Fragments arranged by size:
3714 1078 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2697 487
Size: 683 56 1471 4073
Fragments arranged by size:
4073 1471 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 3799 4593 4613 2390
Size: 1409 794 20 4060
Fragments arranged by size:
4060 1409 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6248
DraI TTT'AAA
Cuts at: 3587 3587
Size: 6283
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5290 809
Size: 507 3974 1802
Fragments arranged by size:
3974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 3730 4619
Size: 54 1425 50 275 18 703 1197 889
Cuts at: 4619 6248 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1197 889 703 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3730 1537
Size: 275 1918 4090
Fragments arranged by size:
4090 1918 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2910 3288 3411 4213 1353
Size: 1557 378 123 802 3423
Fragments arranged by size:
3423 1557 802 378 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4870 5244 5390 387
Size: 1064 138 3281 374 146 1280
Fragments arranged by size:
3281 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2013 2990 3251 4346 4943 5730 5998
Size: 393 381 977 261 1095 597 787 268
Cuts at: 5998 1239
Size: 1524
Fragments arranged by size:
1524 1095 977 787 597 393 381 268
261
EcoO109I rG'GnC_Cy
Cuts at: 2069 2069
Size: 6283
EcoRI G'AATT_C
Cuts at: 3592 3970 3592
Size: 378 5905
FspI TGC'GCA
Cuts at: 4789 4789
Size: 6283
HaeII r_GCGC'y
Cuts at: 1678 5430 1678
Size: 3752 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2675 2707 4924 4956 4998
Size: 32 135 32 1103 32 2217 32 42
Cuts at: 4998 5030 1373
Size: 32 2626
Fragments arranged by size:
2626 2217 1103 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3028 3627 3873 669
Size: 988 281 1090 599 246 3079
Fragments arranged by size:
3079 1090 988 599 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6283
HpaI GTT'AAC
Cuts at: 3873 3873
Size: 6283
KpnI G_GTAC'C
Cuts at: 3608 3608
Size: 6283
MfeI C'AATT_G
Cuts at: 3882 3882
Size: 6283
MluI A'CGCG_T
Cuts at: 6159 6159
Size: 6283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5397 5581 1305
Size: 4092 184 2007
Fragments arranged by size:
4092 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2535 10
Size: 54 1425 343 703 3758
Fragments arranged by size:
3758 1425 703 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2262 2411 2673 3245 4241 4600
Size: 648 960 136 149 262 572 996 359
Cuts at: 4600 4759 518
Size: 159 2042
Fragments arranged by size:
2042 996 960 648 572 359 262 159
149 136
MspA1I CmG'CkG
Cuts at: 1606 2045 2539 3055 3449 3699 4376 5524 5769
Size: 439 494 516 394 250 677 1148 245
Cuts at: 5769 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 516 494 439 394 250
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3082 513
Size: 552 939 1078 3714
Fragments arranged by size:
3714 1078 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6283
NotI GC'GGCC_GC
Cuts at: 3730 3730
Size: 6283
NsiI A_TGCA'T
Cuts at: 3171 3171
Size: 6283
NspI r_CATG'y
Cuts at: 1132 1229 2125 3304 3556 3641 5186 1132
Size: 97 896 1179 252 85 1545 2229
Fragments arranged by size:
2229 1545 1179 896 252 97 85
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6283
PciI A'CATG_T
Cuts at: 2121 5182 2121
Size: 3061 3222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 3472 1108
Size: 1143 1221 3919
Fragments arranged by size:
3919 1221 1143
PmeI GTTT'AAAC
Cuts at: 3587 3587
Size: 6283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4352 4384 5865 5897 4352
Size: 32 1481 32 4738
Fragments arranged by size:
4738 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 2069
Size: 6283
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6283
PsiI TTA'TAA
Cuts at: 3853 3853
Size: 6283
PspOMI G'GGCC_C
Cuts at: 3086 3086
Size: 6283
PstI C_TGCA'G
Cuts at: 830 2500 3542 3635 830
Size: 1670 1042 93 3478
Fragments arranged by size:
3478 1670 1042 93
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6283
PvuII CAG'CTG
Cuts at: 2539 3055 3449 2539
Size: 516 394 5373
Fragments arranged by size:
5373 516 394
SalI G'TCGA_C
Cuts at: 1936 3625 1936
Size: 1689 4594
SapI GCTCTTCn'nnn_
Cuts at: 2910 2910
Size: 6283
SbfI CC_TGCA'GG
Cuts at: 3635 3635
Size: 6283
ScaI AGT'ACT
Cuts at: 4531 6243 4531
Size: 1712 4571
SfcI C'TryA_G
Cuts at: 826 1045 2496 3013 3231 3444 3538 3631 4766
Size: 219 1451 517 218 213 94 93 1135
Cuts at: 4766 5447 5638 826
Size: 681 191 1471
Fragments arranged by size:
1471 1451 1135 681 517 219 218 213
191 94 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 3104 3104
Size: 6283
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6283
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6283
SmaI CCC'GGG
Cuts at: 1872 3610 1872
Size: 1738 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4387 5288 5550 820
Size: 197 926 2444 901 262 1553
Fragments arranged by size:
2444 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4074 493
Size: 3581 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6283
SphI G_CATG'C
Cuts at: 1132 3556 3641 1132
Size: 2424 85 3774
Fragments arranged by size:
3774 2424 85
SspI AAT'ATT
Cuts at: 5 52 4207 5
Size: 47 4155 2081
Fragments arranged by size:
4155 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6283
StyI C'CwwG_G
Cuts at: 513 1065 2004 2483 3082 3337 3722 513
Size: 552 939 479 599 255 385 3074
Fragments arranged by size:
3074 939 599 552 479 385 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3397 3479 4310 4327 4480 4665 5110
Size: 1255 1817 82 831 17 153 185 445
Cuts at: 5110 325
Size: 1498
Fragments arranged by size:
1817 1498 1255 831 445 185 153 82
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 4529 6241
Size: 274 80 33 51 1443 471 2081 1712
Cuts at: 6241 96
Size: 138
Fragments arranged by size:
2081 1712 1443 471 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6243 6275 6243
Size: 32 6251
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6283
XbaI T'CTAG_A
Cuts at: 3619 3619
Size: 6283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 2177
Size: 6283
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6283
XmaI C'CCGG_G
Cuts at: 1870 3608 1870
Size: 1738 4545
XmnI GAAnn'nnTTC
Cuts at: 4412 4412
Size: 6283
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4091 276
Size: 53 83 186 936 2557 2468
Fragments arranged by size:
2557 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfoI SrfI SwaI