Restriction map for
pFN21AA0800
(Circular) (Six-base) MAPSORT of: pFN21AA0800.seq Check: 1419 from: 1 to: 9226
pFN21AA0800 9226 bp
With 182 enzymes: SgfI *
May 17, 2016 16:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5087 5303 5348 5087
Size: 216 45 8965
Fragments arranged by size:
8965 216 45
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7036 278
Size: 53 83 186 936 5500 2468
Fragments arranged by size:
5500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6569 1937
Size: 4632 4594
Acc65I G'GTAC_C
Cuts at: 2098 6547 2098
Size: 4449 4777
AclI AA'CG_TT
Cuts at: 1563 7353 7726 1563
Size: 5790 373 3063
Fragments arranged by size:
5790 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 3110 3351 3617 3789 4034 5375 7289
Size: 531 947 241 266 172 245 1341 1914
Cuts at: 7289 8673 8941 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1914 1384 1341 947 531 268 266
245 241 172
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9029
AflIII A'CryG_T
Cuts at: 1203 2023 3939 8125 9102 1203
Size: 820 1916 4186 977 1327
Fragments arranged by size:
4186 1916 1327 977 820
AgeI A'CCGG_T
Cuts at: 3236 8057 3236
Size: 4821 4405
AhdI GACnn_n'nnGTC
Cuts at: 7955 7955
Size: 9226
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3590 4229 1184
Size: 2406 639 6181
Fragments arranged by size:
6181 2406 639
AlwNI CAG_nnn'CTG
Cuts at: 4353 4369 4676 6184 6301 6969 8541 4353
Size: 16 307 1508 117 668 1572 5038
Fragments arranged by size:
5038 1572 1508 668 307 117 16
ApaI G_GGCC'C
Cuts at: 2413 4273 2413
Size: 1860 7366
ApaLI G'TGCA_C
Cuts at: 3510 7283 8439 3510
Size: 3773 1156 4297
Fragments arranged by size:
4297 3773 1156
ApoI r'AATT_y
Cuts at: 1493 2506 3161 3173 3493 3722 4032 4106 6535
Size: 1013 655 12 320 229 310 74 2429
Cuts at: 6535 6761 6913 9022 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2429 2109 1697 1013 655 320 310 229
226 152 74 12
AseI AT'TA_AT
Cuts at: 160 1032 5829 7780 160
Size: 872 4797 1951 1606
Fragments arranged by size:
4797 1951 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
AvaI C'yCGr_G
Cuts at: 1870 1943 2412 6551 1870
Size: 73 469 4139 4545
Fragments arranged by size:
4545 4139 469 73
BamHI G'GATC_C
Cuts at: 2161 6556 6923 2161
Size: 4395 367 4464
Fragments arranged by size:
4464 4395 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 2942 3901 4763 4949
Size: 325 217 627 311 844 959 862 186
Cuts at: 4949 5223 5336 6547 8002 618
Size: 274 113 1211 1455 1842
Fragments arranged by size:
1842 1455 1211 959 862 844 627 325
311 274 217 186 113
BanII G_rGCy'C
Cuts at: 1391 2046 2301 2413 3432 4273 6545 1391
Size: 655 255 112 1019 841 2272 4072
Fragments arranged by size:
4072 2272 1019 841 655 255 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2542 3443 3842 6432 928
Size: 971 643 901 399 2590 3722
Fragments arranged by size:
3722 2590 971 901 643 399
BbvCI CC'TCA_GC
Cuts at: 4002 4797 4002
Size: 795 8431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7417 7451 1903
Size: 34 5480 34 3678
Fragments arranged by size:
5480 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7118 8328 7118
Size: 1210 8016
BclI T'GATC_A
Cuts at: 1548 3471 5823 1548
Size: 1923 2352 4951
Fragments arranged by size:
4951 2352 1923
BfrBI ATG'CAT
Cuts at: 2276 3301 2276
Size: 1025 8201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2417 7837 136
Size: 107 122 71 631 605 745 5420 1525
Fragments arranged by size:
5420 1525 745 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2718 5656 9221 2718
Size: 2938 3565 2723
Fragments arranged by size:
3565 2938 2723
BlpI GC'TnA_GC
Cuts at: 2040 6643 2040
Size: 4603 4623
Bme1580I G_kGCm'C
Cuts at: 1790 2413 3514 4273 7287 8443 1790
Size: 623 1101 759 3014 1156 2573
Fragments arranged by size:
3014 2573 1156 1101 759 623
BmgBI CAC'GTC
Cuts at: 1318 3940 1318
Size: 2622 6604
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2586 3561 4516
Size: 416 53 83 399 24 1169 975 955
Cuts at: 4516 4664 6957 7915 442
Size: 148 2293 958 1753
Fragments arranged by size:
2293 1753 1169 975 958 955 416 399
148 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3543 4826 6389 1056
Size: 2487 1283 1563 3893
Fragments arranged by size:
3893 2487 1563 1283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2727 3902 5837 6323 7886 1239
Size: 839 649 1175 1935 486 1563 2579
Fragments arranged by size:
2579 1935 1563 1175 839 649 486
Bpu10I CC'TnA_GC
Cuts at: 4002 4797 5118 4002
Size: 795 321 8110
Fragments arranged by size:
8110 795 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7351 8216 8514 7351
Size: 865 298 8063
Fragments arranged by size:
8063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2682 7889 882
Size: 617 1183 5207 2219
Fragments arranged by size:
5207 2219 1183 617
BsaAI yAC'GTr
Cuts at: 493 1204 7017 493
Size: 711 5813 2702
Fragments arranged by size:
5813 2702 711
BsaBI GATnn'nnATC
Cuts at: 2184 2880 4058 4244 9220 2184
Size: 696 1178 186 4976 2190
Fragments arranged by size:
4976 2190 1178 696 186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5727 7033 7415 8050
Size: 53 83 186 936 4194 1306 382 635
Cuts at: 8050 275
Size: 1451
Fragments arranged by size:
4194 1451 1306 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3236 7659 8057 8331 8478 8889 3236
Size: 4423 398 274 147 411 3573
Fragments arranged by size:
4423 3573 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2979 3009 6296 6326 1178
Size: 30 165 30 1576 30 3287 30 4078
Fragments arranged by size:
4078 3287 1576 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 4113 5094 5399 6221 6332 6395 6416
Size: 1465 1462 981 305 822 111 63 21
Cuts at: 6416 6458 1186
Size: 42 3954
Fragments arranged by size:
3954 1465 1462 981 822 305 111 63
42 21
BseYI C'CCAG_C
Cuts at: 2215 3742 4966 8429 8925 2215
Size: 1527 1224 3463 496 2516
Fragments arranged by size:
3463 2516 1527 1224 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5326 5472 5733 1163
Size: 573 3590 146 261 4656
Fragments arranged by size:
4656 3590 573 261 146
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6676 7437 8465 1540
Size: 275 186 4675 761 1028 2301
Fragments arranged by size:
4675 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 3432 3514 4164 4218 7287 7372 8443 2046
Size: 1386 82 650 54 3069 85 1071 2829
Fragments arranged by size:
3069 2829 1386 1071 650 85 82 54
BsmI GAATG_Cn'
Cuts at: 2266 2278 3651 5303 5524 6737 6830 2266
Size: 12 1373 1652 221 1213 93 4662
Fragments arranged by size:
4662 1652 1373 1213 221 93 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2301 2413 3432 3514 4164 4218
Size: 399 256 255 112 1019 82 650 54
Cuts at: 4218 4273 6545 7287 7372 8443 1391
Size: 55 2272 742 85 1071 2174
Fragments arranged by size:
2272 2174 1071 1019 742 650 399 256
255 112 85 82 55 54
BspEI T'CCGG_A
Cuts at: 8889 8889
Size: 9226
BspHI T'CATG_A
Cuts at: 2256 3697 7115 8845 2256
Size: 1441 3418 1730 2637
Fragments arranged by size:
3418 2637 1730 1441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 4638 5087 5303 5348 6581 844
Size: 2728 1066 449 216 45 1233 3489
Fragments arranged by size:
3489 2728 1233 1066 449 216 45
BsrBI CCG'CTC
Cuts at: 1394 1823 5320 5745 5882 7113 1394
Size: 429 3497 425 137 1231 3507
Fragments arranged by size:
3507 3497 1231 429 425 137
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 4728 4912 5598 7721 7895 65
Size: 1183 1233 2247 184 686 2123 174 1396
Fragments arranged by size:
2247 2123 1396 1233 1183 686 184 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3236 7870 8057 1700
Size: 110 1426 4634 187 2869
Fragments arranged by size:
4634 2869 1426 187 110
BsrGI T'GTAC_A
Cuts at: 96 6077 96
Size: 5981 3245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9226
BssSI C'ACGA_G
Cuts at: 7286 8298 7286
Size: 1012 8214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9226
BstBI TT'CG_AA
Cuts at: 2533 5851 8030 2533
Size: 3318 2179 3729
Fragments arranged by size:
3729 3318 2179
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9226
BstXI CCAn_nnnn'nTGG
Cuts at: 3610 5810 3610
Size: 2200 7026
BstYI r'GATC_y
Cuts at: 1972 2161 2718 3956 4359 4530 5656 6556 6923
Size: 189 557 1238 403 171 1126 900 367
Cuts at: 6923 7308 7325 8777 8863 8875 9221 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1238 1126 900 557 403 385
367 346 189 171 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2781 2781
Size: 9226
BtgI C'CryG_G
Cuts at: 513 1065 2597 3282 4802 4945 513
Size: 552 1532 685 1520 143 4794
Fragments arranged by size:
4794 1532 1520 685 552 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8487
Fragments arranged by size:
8487 683 56
BtsI GCAGTG_nn'
Cuts at: 2069 2089 2310 4162 4408 4674 6742 7536 7556
Size: 20 221 1852 246 266 2068 794 20
Cuts at: 7556 2069
Size: 3739
Fragments arranged by size:
3739 2068 1852 794 266 246 221 20
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3157 3192 529
Size: 35 2593 35 6563
Fragments arranged by size:
6563 2593 35 35
DraI TTT'AAA
Cuts at: 2307 6530 2307
Size: 4223 5003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2190 8233 809
Size: 507 874 6043 1802
Fragments arranged by size:
6043 1802 874 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3600 4170 6673
Size: 54 1425 50 275 18 1770 570 2503
Cuts at: 6673 7562 9191 8
Size: 889 1629 43
Fragments arranged by size:
2503 1770 1629 1425 889 570 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6673 1537
Size: 275 4861 4090
Fragments arranged by size:
4861 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2243 2614 5967 6198 6213 7156 1353
Size: 786 104 371 3353 231 15 943 3423
Fragments arranged by size:
3423 3353 943 786 371 231 104 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5223 6323 7813 8187 8333 387
Size: 1064 138 3634 1100 1490 374 146 1280
Fragments arranged by size:
3634 1490 1280 1100 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 3430 2044
Size: 1386 7840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2163 2727 3110 3351 3617 3789
Size: 393 446 85 564 383 241 266 172
Cuts at: 3789 3902 4034 5375 5837 6323 7289 7886 8673
Size: 113 132 1341 462 486 966 597 787
Cuts at: 8673 8941 1239
Size: 268 1524
Fragments arranged by size:
1524 1341 966 787 597 564 486 462
446 393 383 268 266 241 172 132
113 85
EcoNI CCTnn'n_nnAGG
Cuts at: 6398 6398
Size: 9226
EcoO109I rG'GnC_Cy
Cuts at: 4270 5445 5845 4270
Size: 1175 400 7651
Fragments arranged by size:
7651 1175 400
EcoRI G'AATT_C
Cuts at: 6535 6913 6535
Size: 378 8848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4571 5545 4571
Size: 974 8252
FspI TGC'GCA
Cuts at: 3183 3578 7732 3183
Size: 395 4154 4677
Fragments arranged by size:
4677 4154 395
HaeII r_GCGC'y
Cuts at: 1678 5144 8373 1678
Size: 3466 3229 2531
Fragments arranged by size:
3466 3229 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2979 3011 3941 3948 3973
Size: 32 135 32 1407 32 930 7 25
Cuts at: 3973 3980 4880 4912 5729 5761 6413 6445 7867
Size: 7 900 32 817 32 652 32 1422
Cuts at: 7867 7899 7941 7973 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 1407 930 900 817 652 135
42 32 32 32 32 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 5640 6570 6816 669
Size: 988 281 3702 930 246 3079
Fragments arranged by size:
3702 3079 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 2700 748
Size: 1952 7274
HpaI GTT'AAC
Cuts at: 6816 6816
Size: 9226
KpnI G_GTAC'C
Cuts at: 2102 6551 2102
Size: 4449 4777
MfeI C'AATT_G
Cuts at: 2123 3829 4063 6825 2123
Size: 1706 234 2762 4524
Fragments arranged by size:
4524 2762 1706 234
MluI A'CGCG_T
Cuts at: 9102 9102
Size: 9226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3924 4221 4920 6294 8340 8524 1305
Size: 2619 297 699 1374 2046 184 2007
Fragments arranged by size:
2619 2046 2007 1374 699 297 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3602 4172 10
Size: 54 1425 343 1770 570 5064
Fragments arranged by size:
5064 1770 1425 570 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2082 3044 4211 4238 5610 5808 6050
Size: 648 916 962 1167 27 1372 198 242
Cuts at: 6050 7184 7543 7702 518
Size: 1134 359 159 2042
Fragments arranged by size:
2042 1372 1167 1134 962 916 648 359
242 198 159 27
MspA1I CmG'CkG
Cuts at: 1606 2515 3767 4353 4369 4984 5353 6642 7319
Size: 909 1252 586 16 615 369 1289 677
Cuts at: 7319 8467 8712 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1289 1252 1148 909 677 615 586
369 245 16
NcoI C'CATG_G
Cuts at: 513 1065 3282 4945 513
Size: 552 2217 1663 4794
Fragments arranged by size:
4794 2217 1663 552
NdeI CA'TA_TG
Cuts at: 387 2278 387
Size: 1891 7335
NheI G'CTAG_C
Cuts at: 1052 3539 4822 6385 1052
Size: 2487 1283 1563 3893
Fragments arranged by size:
3893 2487 1563 1283
NotI GC'GGCC_GC
Cuts at: 6673 6673
Size: 9226
NsiI A_TGCA'T
Cuts at: 2278 3303 2278
Size: 1025 8201
NspI r_CATG'y
Cuts at: 1132 1229 2027 2890 3301 3687 5054 6584 8129
Size: 97 798 863 411 386 1367 1530 1545
Cuts at: 8129 1132
Size: 2229
Fragments arranged by size:
2229 1545 1530 1367 863 798 411 386
97
PasI CC'CwG_GG
Cuts at: 1348 2931 4813 1348
Size: 1583 1882 5761
Fragments arranged by size:
5761 1882 1583
PciI A'CATG_T
Cuts at: 2023 8125 2023
Size: 6102 3124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4179 5192 6301 1108
Size: 3071 1013 1109 4033
Fragments arranged by size:
4033 3071 1109 1013
PfoI T'CCnGG_A
Cuts at: 5262 5262
Size: 9226
PmeI GTTT'AAAC
Cuts at: 6530 6530
Size: 9226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 7295 7327 8808 8840 3995
Size: 32 3268 32 1481 32 4381
Fragments arranged by size:
4381 3268 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5445 5845 5445
Size: 400 8826
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9226
PsiI TTA'TAA
Cuts at: 6796 6796
Size: 9226
PspOMI G'GGCC_C
Cuts at: 2409 4269 2409
Size: 1860 7366
PstI C_TGCA'G
Cuts at: 830 2317 4392 4503 6578 830
Size: 1487 2075 111 2075 3478
Fragments arranged by size:
3478 2075 2075 1487 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9226
PvuII CAG'CTG
Cuts at: 2515 3767 4353 4369 4984 5353 2515
Size: 1252 586 16 615 369 6388
Fragments arranged by size:
6388 1252 615 586 369 16
SacI G_AGCT'C
Cuts at: 2046 3432 2046
Size: 1386 7840
SalI G'TCGA_C
Cuts at: 1936 6568 1936
Size: 4632 4594
SbfI CC_TGCA'GG
Cuts at: 6578 6578
Size: 9226
ScaI AGT'ACT
Cuts at: 3478 7474 9186 3478
Size: 3996 1712 3518
Fragments arranged by size:
3996 3518 1712
SfcI C'TryA_G
Cuts at: 826 1045 2010 2313 2494 2549 2555 2670 3960
Size: 219 965 303 181 55 6 115 1290
Cuts at: 3960 3972 4388 4499 4954 5700 6036 6574 7709
Size: 12 416 111 455 746 336 538 1135
Cuts at: 7709 8390 8581 826
Size: 681 191 1471
Fragments arranged by size:
1471 1290 1135 965 746 681 538 455
416 336 303 219 191 181 115 111
55 12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2417 2417
Size: 9226
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9226
SmaI CCC'GGG
Cuts at: 1872 6553 1872
Size: 4681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 7330 8231 8493 820
Size: 197 926 5387 901 262 1553
Fragments arranged by size:
5387 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7017 493
Size: 6524 2702
SpeI A'CTAG_T
Cuts at: 152 2750 152
Size: 2598 6628
SphI G_CATG'C
Cuts at: 1132 3301 3687 6584 1132
Size: 2169 386 2897 3774
Fragments arranged by size:
3774 2897 2169 386
SspI AAT'ATT
Cuts at: 5 52 3002 3534 7150 5
Size: 47 2950 532 3616 2081
Fragments arranged by size:
3616 2950 2081 532 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9226
StyI C'CwwG_G
Cuts at: 513 1065 3282 4945 6665 513
Size: 552 2217 1663 1720 3074
Fragments arranged by size:
3074 2217 1720 1663 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2923 3678 4751 7253 7270 7423 7608
Size: 1255 1343 755 1073 2502 17 153 185
Cuts at: 7608 8053 325
Size: 445 1498
Fragments arranged by size:
2502 1498 1343 1255 1073 755 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3193 3476 6077
Size: 274 80 33 51 1443 1216 283 2601
Cuts at: 6077 7472 9184 96
Size: 1395 1712 138
Fragments arranged by size:
2601 1712 1443 1395 1216 283 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3631 3663 5680 5712 5742 5774 5805 5837 9186
Size: 32 2017 32 30 32 31 32 3349
Cuts at: 9186 9218 3631
Size: 32 3639
Fragments arranged by size:
3639 3349 2017 32 32 32 32 32
31 30
Tth111I GACn'n_nGTC
Cuts at: 1316 4409 1316
Size: 3093 6133
XbaI T'CTAG_A
Cuts at: 6397 6562 6397
Size: 165 9061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2854 2854
Size: 9226
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9226
XmaI C'CCGG_G
Cuts at: 1870 6551 1870
Size: 4681 4545
XmnI GAAnn'nnTTC
Cuts at: 2399 7355 2399
Size: 4956 4270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7034 276
Size: 53 83 186 936 5500 2468
Fragments arranged by size:
5500 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfoI SrfI SwaI