Restriction map for
pFN21AA0791
(Circular) (Six-base) MAPSORT of: pFN21AA0791.seq Check: 2634 from: 1 to: 8701
pFN21AA0791 8701 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2909 2909
Size: 8701
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6511 278
Size: 53 83 186 936 4975 2468
Fragments arranged by size:
4975 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2340 3706 4965 5806 6044 1937
Size: 403 1366 1259 841 238 4594
Fragments arranged by size:
4594 1366 1259 841 403 238
Acc65I G'GTAC_C
Cuts at: 6022 6022
Size: 8701
AclI AA'CG_TT
Cuts at: 1563 6828 7201 1563
Size: 5265 373 3063
Fragments arranged by size:
5265 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 4145 4241 4301 4697 5144 5276 6764
Size: 942 1571 96 60 396 447 132 1488
Cuts at: 6764 8148 8416 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1571 1488 1384 942 447 396 268
132 96 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8504
AflIII A'CryG_T
Cuts at: 1203 3306 5809 7600 8577 1203
Size: 2103 2503 1791 977 1327
Fragments arranged by size:
2503 2103 1791 1327 977
AgeI A'CCGG_T
Cuts at: 7532 7532
Size: 8701
AhdI GACnn_n'nnGTC
Cuts at: 7430 7430
Size: 8701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8701
AlwNI CAG_nnn'CTG
Cuts at: 2387 4060 4114 4201 6444 8016 2387
Size: 1673 54 87 2243 1572 3072
Fragments arranged by size:
3072 2243 1673 1572 87 54
ApaLI G'TGCA_C
Cuts at: 6758 7914 6758
Size: 1156 7545
ApoI r'AATT_y
Cuts at: 1493 2502 2875 3323 3983 5151 5243 5399 5586
Size: 1009 373 448 660 1168 92 156 187
Cuts at: 5586 5962 6010 6236 6388 8497 1493
Size: 376 48 226 152 2109 1697
Fragments arranged by size:
2109 1697 1168 1009 660 448 376 373
226 187 156 152 92 48
AseI AT'TA_AT
Cuts at: 160 1032 4337 7255 160
Size: 872 3305 2918 1606
Fragments arranged by size:
3305 2918 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
AvaI C'yCGr_G
Cuts at: 1870 1943 3537 5184 6026 1870
Size: 73 1594 1647 842 4545
Fragments arranged by size:
4545 1647 1594 842 73
BamHI G'GATC_C
Cuts at: 5283 5758 6031 6398 5283
Size: 475 273 367 7586
Fragments arranged by size:
7586 475 367 273
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6022 7477 618
Size: 325 217 627 4235 1455 1842
Fragments arranged by size:
4235 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2606 3061 3208 4929 5537 5919 5933 6020
Size: 1215 455 147 1721 608 382 14 87
Cuts at: 6020 1391
Size: 4072
Fragments arranged by size:
4072 1721 1215 608 455 382 147 87
14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3465 4704 928
Size: 971 1566 1239 4925
Fragments arranged by size:
4925 1566 1239 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4163 4197 6892 6926 1903
Size: 34 2226 34 2695 34 3678
Fragments arranged by size:
3678 2695 2226 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6593 7803 6593
Size: 1210 7491
BclI T'GATC_A
Cuts at: 1548 5038 5737 1548
Size: 3490 699 4512
Fragments arranged by size:
4512 3490 699
BfrBI ATG'CAT
Cuts at: 4749 5140 5551 4749
Size: 391 411 7899
Fragments arranged by size:
7899 411 391
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2549 7312 136
Size: 107 122 71 631 605 877 4763 1525
Fragments arranged by size:
4763 1525 877 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4300 8696 4300
Size: 4396 4305
BlpI GC'TnA_GC
Cuts at: 6118 6118
Size: 8701
Bme1580I G_kGCm'C
Cuts at: 1790 6762 7918 1790
Size: 4972 1156 2573
Fragments arranged by size:
4972 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3997 4800 5674
Size: 416 53 83 399 24 2580 803 874
Cuts at: 5674 5967 6432 7390 442
Size: 293 465 958 1753
Fragments arranged by size:
2580 1753 958 874 803 465 416 399
293 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2192 2108
Size: 84 8617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 3714 4454 4815 5027 5903 7361 1239
Size: 1170 1305 740 361 212 876 1458 2579
Fragments arranged by size:
2579 1458 1305 1170 876 740 361 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4638 5549 6826 7691 7989 4638
Size: 911 1277 865 298 5350
Fragments arranged by size:
5350 1277 911 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2217 3630 7364 882
Size: 617 718 1413 3734 2219
Fragments arranged by size:
3734 2219 1413 718 617
BsaAI yAC'GTr
Cuts at: 493 1204 6492 493
Size: 711 5288 2702
Fragments arranged by size:
5288 2702 711
BsaBI GATnn'nnATC
Cuts at: 8695 8695
Size: 8701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6508 6890 7525 275
Size: 53 83 186 936 4975 382 635 1451
Fragments arranged by size:
4975 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7134 7532 7806 7953 8364 7134
Size: 398 274 147 411 7471
Fragments arranged by size:
7471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3983 4013 1178
Size: 30 165 30 2580 30 5866
Fragments arranged by size:
5866 2580 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2235 3323 3429 4785 5527 1186
Size: 1049 1088 106 1356 742 4360
Fragments arranged by size:
4360 1356 1088 1049 742 106
BseYI C'CCAG_C
Cuts at: 3751 4095 5840 7904 8400 3751
Size: 344 1745 2064 496 4052
Fragments arranged by size:
4052 2064 1745 496 344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4070 5498 5774 1163
Size: 573 2334 1428 276 4090
Fragments arranged by size:
4090 2334 1428 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 6151 6912 7940 1540
Size: 275 186 832 3318 761 1028 2301
Fragments arranged by size:
3318 2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2606 3061 3742 4582 4929 5537 5919 5933 6762
Size: 455 681 840 347 608 382 14 829
Cuts at: 6762 6847 7918 2606
Size: 85 1071 3389
Fragments arranged by size:
3389 1071 840 829 681 608 455 382
347 85 14
BsmI GAATG_Cn'
Cuts at: 2424 2794 5553 6212 6305 2424
Size: 370 2759 659 93 4820
Fragments arranged by size:
4820 2759 659 370 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3702 3702
Size: 8701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2606 3061 3208 3742 4582 4929 5537
Size: 399 816 455 147 534 840 347 608
Cuts at: 5537 5919 5933 6020 6762 6847 7918 1391
Size: 382 14 87 742 85 1071 2174
Fragments arranged by size:
2174 1071 840 816 742 608 534 455
399 382 347 147 87 85 14
BspEI T'CCGG_A
Cuts at: 8364 8364
Size: 8701
BspHI T'CATG_A
Cuts at: 4129 5251 6590 8320 4129
Size: 1122 1339 1730 4510
Fragments arranged by size:
4510 1730 1339 1122
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 5638 6056 844
Size: 2065 2729 418 3489
Fragments arranged by size:
3489 2729 2065 418
BsrBI CCG'CTC
Cuts at: 1394 1823 4793 6588 1394
Size: 429 2970 1795 3507
Fragments arranged by size:
3507 2970 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2814 5178 5711 7196 7370 65
Size: 1183 1566 2364 533 1485 174 1396
Fragments arranged by size:
2364 1566 1485 1396 1183 533 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5453 7345 7532 1700
Size: 110 3643 1892 187 2869
Fragments arranged by size:
3643 2869 1892 187 110
BsrGI T'GTAC_A
Cuts at: 96 3295 5338 5629 96
Size: 3199 2043 291 3168
Fragments arranged by size:
3199 3168 2043 291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8701
BssSI C'ACGA_G
Cuts at: 5112 6761 7773 5112
Size: 1649 1012 6040
Fragments arranged by size:
6040 1649 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4340 794
Size: 3546 5155
BstBI TT'CG_AA
Cuts at: 4183 7505 4183
Size: 3322 5379
BstEII G'GTnAC_C
Cuts at: 1184 5903 1184
Size: 4719 3982
BstYI r'GATC_y
Cuts at: 1972 2434 4300 4648 5283 5758 6031 6398 6783
Size: 462 1866 348 635 475 273 367 385
Cuts at: 6783 6800 8252 8338 8350 8696 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1866 1452 635 475 462 385 367
348 346 273 86 17 12
BstZ17I GTA'TAC
Cuts at: 3707 5807 3707
Size: 2100 6601
BtgI C'CryG_G
Cuts at: 513 1065 3030 513
Size: 552 1965 6184
Fragments arranged by size:
6184 1965 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7962
Fragments arranged by size:
7962 683 56
BtsI GCAGTG_nn'
Cuts at: 2135 2871 3498 3912 5211 5786 6217 7011 7031
Size: 736 627 414 1299 575 431 794 20
Cuts at: 7031 2135
Size: 3805
Fragments arranged by size:
3805 1299 794 736 627 575 431 414
20
ClaI AT'CG_AT
Cuts at: 1554 2829 1554
Size: 1275 7426
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8666
DraI TTT'AAA
Cuts at: 2845 3255 3445 4297 5416 5853 5968 6005 2845
Size: 410 190 852 1119 437 115 37 5541
Fragments arranged by size:
5541 1119 852 437 410 190 115 37
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7708 809
Size: 507 6392 1802
Fragments arranged by size:
6392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5028 6148 7037
Size: 54 1425 50 275 18 3198 1120 889
Cuts at: 7037 8666 8
Size: 1629 43
Fragments arranged by size:
3198 1629 1425 1120 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6148 1537
Size: 275 4336 4090
Fragments arranged by size:
4336 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2077 2454 3420 3594 5979 6631 1353
Size: 724 377 966 174 2385 652 3423
Fragments arranged by size:
3423 2385 966 724 652 377 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3582 7288 7662 7808 387
Size: 1064 138 1993 3706 374 146 1280
Fragments arranged by size:
3706 1993 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2604 3059 4927 5535 5917 5931 2604
Size: 455 1868 608 382 14 5374
Fragments arranged by size:
5374 1868 608 455 382 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2574 3714 4145 4241 4301 4454
Size: 393 777 165 1140 431 96 60 153
Cuts at: 4454 4697 4815 5027 5144 5276 5903 6764 7361
Size: 243 118 212 117 132 627 861 597
Cuts at: 7361 8148 8416 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1140 861 787 777 627 597 431
393 268 243 212 165 153 132 118
117 96 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2028 2028
Size: 8701
EcoO109I rG'GnC_Cy
Cuts at: 2033 2033
Size: 8701
EcoRI G'AATT_C
Cuts at: 6010 6388 6010
Size: 378 8323
EcoRV GAT'ATC
Cuts at: 4411 5497 4411
Size: 1086 7615
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4580 4985 4580
Size: 405 8296
FspI TGC'GCA
Cuts at: 7207 7207
Size: 8701
HaeII r_GCGC'y
Cuts at: 1678 7848 1678
Size: 6170 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2815 2847 3983
Size: 32 135 32 821 32 390 32 1136
Cuts at: 3983 4015 5906 5938 7342 7374 7416 7448 1373
Size: 32 1891 32 1404 32 42 32 2626
Fragments arranged by size:
2626 1891 1404 1136 821 390 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3130 3726 4966 6045 6291 669
Size: 988 281 1192 596 1240 1079 246 3079
Fragments arranged by size:
3079 1240 1192 1079 988 596 281 246
HindIII A'AGCT_T
Cuts at: 748 3868 5387 748
Size: 3120 1519 4062
Fragments arranged by size:
4062 3120 1519
HpaI GTT'AAC
Cuts at: 6291 6291
Size: 8701
KpnI G_GTAC'C
Cuts at: 6026 6026
Size: 8701
MfeI C'AATT_G
Cuts at: 2181 2676 2850 3918 6300 2181
Size: 495 174 1068 2382 4582
Fragments arranged by size:
4582 2382 1068 495 174
MluI A'CGCG_T
Cuts at: 8577 8577
Size: 8701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3605 4687 5311 7815 7999 1305
Size: 2300 1082 624 2504 184 2007
Fragments arranged by size:
2504 2300 2007 1082 624 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5030 10
Size: 54 1425 343 3198 3681
Fragments arranged by size:
3681 3198 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2919 3770 6659 7018 7177 518
Size: 648 1753 851 2889 359 159 2042
Fragments arranged by size:
2889 2042 1753 851 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3182 4279 4379 5359 6117 6794 7942 8187
Size: 1576 1097 100 980 758 677 1148 245
Cuts at: 8187 1606
Size: 2120
Fragments arranged by size:
2120 1576 1148 1097 980 758 677 245
100
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8149
NdeI CA'TA_TG
Cuts at: 387 2124 387
Size: 1737 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8701
NotI GC'GGCC_GC
Cuts at: 6148 6148
Size: 8701
NsiI A_TGCA'T
Cuts at: 4751 5142 5553 4751
Size: 391 411 7899
Fragments arranged by size:
7899 411 391
NspI r_CATG'y
Cuts at: 1132 1229 2918 3310 5140 5555 5813 6059 7604
Size: 97 1689 392 1830 415 258 246 1545
Cuts at: 7604 1132
Size: 2229
Fragments arranged by size:
2229 1830 1689 1545 415 392 258 246
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8701
PciI A'CATG_T
Cuts at: 3306 5809 7600 3306
Size: 2503 1791 4407
Fragments arranged by size:
4407 2503 1791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8701
PfoI T'CCnGG_A
Cuts at: 3007 3007
Size: 8701
PmeI GTTT'AAAC
Cuts at: 6005 6005
Size: 8701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3682 3714 6770 6802 8283 8315 3682
Size: 32 3056 32 1481 32 4068
Fragments arranged by size:
4068 3056 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2033 2033
Size: 8701
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8701
PsiI TTA'TAA
Cuts at: 4947 6271 4947
Size: 1324 7377
PspXI vC'TCGA_Gb
Cuts at: 5184 5184
Size: 8701
PstI C_TGCA'G
Cuts at: 830 4119 4639 6053 830
Size: 3289 520 1414 3478
Fragments arranged by size:
3478 3289 1414 520
PvuI CG_AT'CG
Cuts at: 2001 2833 2001
Size: 832 7869
PvuII CAG'CTG
Cuts at: 3182 4279 5359 3182
Size: 1097 1080 6524
Fragments arranged by size:
6524 1097 1080
SacI G_AGCT'C
Cuts at: 2606 3061 4929 5537 5919 5933 2606
Size: 455 1868 608 382 14 5374
Fragments arranged by size:
5374 1868 608 455 382 14
SalI G'TCGA_C
Cuts at: 1936 4964 6043 1936
Size: 3028 1079 4594
Fragments arranged by size:
4594 3028 1079
SbfI CC_TGCA'GG
Cuts at: 6053 6053
Size: 8701
ScaI AGT'ACT
Cuts at: 6949 8661 6949
Size: 1712 6989
SexAI A'CCwGG_T
Cuts at: 2035 4822 2035
Size: 2787 5914
SfcI C'TryA_G
Cuts at: 826 1045 2337 3375 4115 4635 4985 6049 7184
Size: 219 1292 1038 740 520 350 1064 1135
Cuts at: 7184 7865 8056 826
Size: 681 191 1471
Fragments arranged by size:
1471 1292 1135 1064 1038 740 681 520
350 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8701
SmaI CCC'GGG
Cuts at: 1872 6028 1872
Size: 4156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4653 5184 5564 6805 7706 7968
Size: 197 926 2710 531 380 1241 901 262
Cuts at: 7968 820
Size: 1553
Fragments arranged by size:
2710 1553 1241 926 901 531 380 262
197
SnaBI TAC'GTA
Cuts at: 493 6492 493
Size: 5999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8701
SphI G_CATG'C
Cuts at: 1132 5555 6059 1132
Size: 4423 504 3774
Fragments arranged by size:
4423 3774 504
SspI AAT'ATT
Cuts at: 5 52 2695 3888 6625 5
Size: 47 2643 1193 2737 2081
Fragments arranged by size:
2737 2643 2081 1193 47
StuI AGG'CCT
Cuts at: 1517 4718 1517
Size: 3201 5500
StyI C'CwwG_G
Cuts at: 513 1065 4917 5578 6140 513
Size: 552 3852 661 562 3074
Fragments arranged by size:
3852 3074 661 562 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2135 6728 6745 6898 7083 7528 325
Size: 1255 555 4593 17 153 185 445 1498
Fragments arranged by size:
4593 1498 1255 555 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3049 3295 3801
Size: 274 80 33 51 1443 1072 246 506
Cuts at: 3801 5116 5175 5338 5629 5654 5944 6947 8659
Size: 1315 59 163 291 25 290 1003 1712
Cuts at: 8659 96
Size: 138
Fragments arranged by size:
1712 1443 1315 1072 1003 506 291 290
274 246 163 138 80 59 51 33
25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 5004 5036 8661 8693 2598
Size: 32 2374 32 3625 32 2606
Fragments arranged by size:
3625 2606 2374 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8701
XbaI T'CTAG_A
Cuts at: 2687 3988 6037 2687
Size: 1301 2049 5351
Fragments arranged by size:
5351 2049 1301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3179 2011
Size: 1168 7533
XhoI C'TCGA_G
Cuts at: 1943 5184 1943
Size: 3241 5460
XmaI C'CCGG_G
Cuts at: 1870 6026 1870
Size: 4156 4545
XmnI GAAnn'nnTTC
Cuts at: 5247 5994 6830 5247
Size: 747 836 7118
Fragments arranged by size:
7118 836 747
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6509 276
Size: 53 83 186 936 4975 2468
Fragments arranged by size:
4975 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI Bsu36I DraIII FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI
RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI