Restriction map for
pFN21AA0777
(Circular) (Six-base) MAPSORT of: pFN21AA0777.seq Check: 1265 from: 1 to: 8005
pFN21AA0777 8005 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3972 3972
Size: 8005
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5815 278
Size: 53 83 186 936 4279 2468
Fragments arranged by size:
4279 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2277 4674 5348 1937
Size: 340 2397 674 4594
Fragments arranged by size:
4594 2397 674 340
Acc65I G'GTAC_C
Cuts at: 5326 5326
Size: 8005
AclI AA'CG_TT
Cuts at: 1563 6132 6505 1563
Size: 4569 373 3063
Fragments arranged by size:
4569 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2657 2746 3374 6068 7452 7720 1632
Size: 1025 89 628 2694 1384 268 1917
Fragments arranged by size:
2694 1917 1384 1025 628 268 89
AfeI AGC'GCT
Cuts at: 4335 4335
Size: 8005
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7808
AflIII A'CryG_T
Cuts at: 1203 2352 2518 3778 6904 7881 1203
Size: 1149 166 1260 3126 977 1327
Fragments arranged by size:
3126 1327 1260 1149 977 166
AgeI A'CCGG_T
Cuts at: 6836 6836
Size: 8005
AhdI GACnn_n'nnGTC
Cuts at: 3595 4513 6734 3595
Size: 918 2221 4866
Fragments arranged by size:
4866 2221 918
AleI CACnn'nnGTG
Cuts at: 1166 4865 1166
Size: 3699 4306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2093 1184
Size: 909 7096
AlwNI CAG_nnn'CTG
Cuts at: 2134 3972 4473 5149 5748 7320 2134
Size: 1838 501 676 599 1572 2819
Fragments arranged by size:
2819 1838 1572 676 599 501
ApaI G_GGCC'C
Cuts at: 2235 2235
Size: 8005
ApaLI G'TGCA_C
Cuts at: 2327 4497 4560 6062 7218 2327
Size: 2170 63 1502 1156 3114
Fragments arranged by size:
3114 2170 1502 1156 63
ApoI r'AATT_y
Cuts at: 1493 2316 2615 3539 3577 4135 5264 5314 5540
Size: 823 299 924 38 558 1129 50 226
Cuts at: 5540 5692 7801 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1129 924 823 558 299 226
152 50 38
AseI AT'TA_AT
Cuts at: 160 1032 6559 160
Size: 872 5527 1606
Fragments arranged by size:
5527 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8005
AvaI C'yCGr_G
Cuts at: 1870 1943 3039 3343 3510 3699 3771 4310 5330
Size: 73 1096 304 167 189 72 539 1020
Cuts at: 5330 1870
Size: 4545
Fragments arranged by size:
4545 1096 1020 539 304 189 167 73
72
BamHI G'GATC_C
Cuts at: 2584 3914 5335 5702 2584
Size: 1330 1421 367 4887
Fragments arranged by size:
4887 1421 1330 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 3505 3520 3733 3984
Size: 325 217 627 354 1364 15 213 251
Cuts at: 3984 5326 6781 618
Size: 1342 1455 1842
Fragments arranged by size:
1842 1455 1364 1342 627 354 325 251
217 213 15
BanII G_rGCy'C
Cuts at: 1391 2235 2976 3224 4059 4349 5081 5190 5324
Size: 844 741 248 835 290 732 109 134
Cuts at: 5324 1391
Size: 4072
Fragments arranged by size:
4072 844 835 741 732 290 248 134
109
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 928
Size: 971 654 6380
Fragments arranged by size:
6380 971 654
BbvCI CC'TCA_GC
Cuts at: 4337 5016 4337
Size: 679 7326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2077 2111 6196 6230 1903
Size: 34 140 34 4085 34 3678
Fragments arranged by size:
4085 3678 140 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5897 7107 5897
Size: 1210 6795
BclI T'GATC_A
Cuts at: 1548 3877 4693 4918 1548
Size: 2329 816 225 4635
Fragments arranged by size:
4635 2329 816 225
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3711 4063 6616
Size: 107 122 71 631 605 2039 352 2553
Cuts at: 6616 136
Size: 1525
Fragments arranged by size:
2553 2039 1525 631 605 352 122 107
71
BglII A'GATC_T
Cuts at: 8000 8000
Size: 8005
BlpI GC'TnA_GC
Cuts at: 5422 5422
Size: 8005
Bme1580I G_kGCm'C
Cuts at: 1790 2235 2331 3510 3525 3738 3989 4501 4564
Size: 445 96 1179 15 213 251 512 63
Cuts at: 4564 6066 7222 1790
Size: 1502 1156 2573
Fragments arranged by size:
2573 1502 1179 1156 512 445 251 213
96 63 15
BmgBI CAC'GTC
Cuts at: 1318 4453 1318
Size: 3135 4870
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2498 2590 5736
Size: 416 53 83 399 24 1081 92 3146
Cuts at: 5736 6694 442
Size: 958 1753
Fragments arranged by size:
3146 1753 1081 958 416 399 92 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8005
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2870 3259 4336 2870
Size: 389 1077 6539
Fragments arranged by size:
6539 1077 389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2050 2514 2574 2974 3127 3502 3689 4569
Size: 811 464 60 400 153 375 187 880
Cuts at: 4569 5204 6665 1239
Size: 635 1461 2579
Fragments arranged by size:
2579 1461 880 811 635 464 400 375
187 153 60
Bpu10I CC'TnA_GC
Cuts at: 4337 5016 4337
Size: 679 7326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5095 5236 6130 6995 7293 5095
Size: 141 894 865 298 5807
Fragments arranged by size:
5807 894 865 298 141
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6668 882
Size: 617 5169 2219
Fragments arranged by size:
5169 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3781 4753 5796 493
Size: 711 2577 972 1043 2702
Fragments arranged by size:
2702 2577 1043 972 711
BsaBI GATnn'nnATC
Cuts at: 7999 7999
Size: 8005
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5812 6194 6829 275
Size: 53 83 186 936 4279 382 635 1451
Fragments arranged by size:
4279 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2246 4122 6438 6836 7110 7257 7668 2246
Size: 1876 2316 398 274 147 411 2583
Fragments arranged by size:
2583 2316 1876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3115 3145 4656 4686 4881
Size: 30 165 30 1712 30 1511 30 195
Cuts at: 4881 4911 1178
Size: 30 4272
Fragments arranged by size:
4272 1712 1511 195 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3251 3515 4067 4354 4357 1186
Size: 2065 264 552 287 3 4834
Fragments arranged by size:
4834 2065 552 287 264 3
BseYI C'CCAG_C
Cuts at: 2050 2118 4809 7208 7704 2050
Size: 68 2691 2399 496 2351
Fragments arranged by size:
2691 2399 2351 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4259 4337 1163
Size: 573 2523 78 4831
Fragments arranged by size:
4831 2523 573 78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 5455 6216 7244 1540
Size: 275 186 280 3174 761 1028 2301
Fragments arranged by size:
3174 2301 1028 761 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 2410 3494 3778 4501 4564 5081 5190 6066
Size: 79 1084 284 723 63 517 109 876
Cuts at: 6066 6151 7222 2331
Size: 85 1071 3114
Fragments arranged by size:
3114 1084 1071 876 723 517 284 109
85 79 63
BsmI GAATG_Cn'
Cuts at: 4431 5516 5609 4431
Size: 1085 93 6827
Fragments arranged by size:
6827 1085 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3133 3133
Size: 8005
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2331 2410 2976 3224 3494 3510
Size: 399 445 96 79 566 248 270 16
Cuts at: 3510 3525 3738 3778 3989 4059 4349 4501 4564
Size: 15 213 40 211 70 290 152 63
Cuts at: 4564 5081 5190 5324 6066 6151 7222 1391
Size: 517 109 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 517 445 399 290
270 248 213 211 152 134 109 96
85 79 70 63 40 16 15
BspEI T'CCGG_A
Cuts at: 2246 4122 7668 2246
Size: 1876 3546 2583
Fragments arranged by size:
3546 2583 1876
BspHI T'CATG_A
Cuts at: 5110 5894 7624 5110
Size: 784 1730 5491
Fragments arranged by size:
5491 1730 784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2372 3477 3972 5360 844
Size: 1528 1105 495 1388 3489
Fragments arranged by size:
3489 1528 1388 1105 495
BsrBI CCG'CTC
Cuts at: 1394 1823 3698 5892 1394
Size: 429 1875 2194 3507
Fragments arranged by size:
3507 2194 1875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6500 6674 65
Size: 1183 5252 174 1396
Fragments arranged by size:
5252 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2280 3705 6649 6836 1700
Size: 110 470 1425 2944 187 2869
Fragments arranged by size:
2944 2869 1425 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 2388 3360 96
Size: 2292 972 4741
Fragments arranged by size:
4741 2292 972
BssHII G'CGCG_C
Cuts at: 1925 3475 1925
Size: 1550 6455
BssSI C'ACGA_G
Cuts at: 6065 7077 6065
Size: 1012 6993
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4787 794
Size: 3993 4012
BstBI TT'CG_AA
Cuts at: 3543 6809 3543
Size: 3266 4739
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8005
BstXI CCAn_nnnn'nTGG
Cuts at: 4490 4490
Size: 8005
BstYI r'GATC_y
Cuts at: 1972 2584 3914 5335 5702 6087 6104 7556 7642
Size: 612 1330 1421 367 385 17 1452 86
Cuts at: 7642 7654 8000 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1421 1330 612 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4682 4682
Size: 8005
BtgI C'CryG_G
Cuts at: 513 1065 2235 2801 3261 3495 4489 4720 513
Size: 552 1170 566 460 234 994 231 3798
Fragments arranged by size:
3798 1170 994 566 552 460 234 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3774 487
Size: 683 56 2548 4718
Fragments arranged by size:
4718 2548 683 56
BtsI GCAGTG_nn'
Cuts at: 2796 3868 4370 4441 5521 6315 6335 2796
Size: 1072 502 71 1080 794 20 4466
Fragments arranged by size:
4466 1080 1072 794 502 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8005
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2790 2825 529
Size: 35 2226 35 5709
Fragments arranged by size:
5709 2226 35 35
DraI TTT'AAA
Cuts at: 5309 5309
Size: 8005
DraIII CAC_nnn'GTG
Cuts at: 2326 2326
Size: 8005
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3200 7012 809
Size: 507 1884 3812 1802
Fragments arranged by size:
3812 1884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2751 5452 6341
Size: 54 1425 50 275 18 921 2701 889
Cuts at: 6341 7970 8
Size: 1629 43
Fragments arranged by size:
2701 1629 1425 921 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5452 1537
Size: 275 3640 4090
Fragments arranged by size:
4090 3640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2183 2771 2902 4570 5935 1353
Size: 825 5 588 131 1668 1365 3423
Fragments arranged by size:
3423 1668 1365 825 588 131 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4791 6592 6966 7112 387
Size: 1064 138 3202 1801 374 146 1280
Fragments arranged by size:
3202 1801 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5079 5188 5079
Size: 109 7896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2050 2514 2574 2657 2746 2974 3127
Size: 393 418 464 60 83 89 228 153
Cuts at: 3127 3374 3502 3689 4569 5204 6068 6665 7452
Size: 247 128 187 880 635 864 597 787
Cuts at: 7452 7720 1239
Size: 268 1524
Fragments arranged by size:
1524 880 864 787 635 597 464 418
393 268 247 228 187 153 128 89
83 60
EcoO109I rG'GnC_Cy
Cuts at: 3273 3391 3466 5247 3273
Size: 118 75 1781 6031
Fragments arranged by size:
6031 1781 118 75
EcoRI G'AATT_C
Cuts at: 3539 5314 5692 3539
Size: 1775 378 5852
Fragments arranged by size:
5852 1775 378
FspI TGC'GCA
Cuts at: 2127 6511 2127
Size: 4384 3621
HaeII r_GCGC'y
Cuts at: 1678 4337 7152 1678
Size: 2659 2815 2531
Fragments arranged by size:
2815 2659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2184 2216 2483
Size: 32 135 32 427 32 153 32 267
Cuts at: 2483 2515 3300 3332 3581 3613 3836 3868 4499
Size: 32 785 32 249 32 223 32 631
Cuts at: 4499 4531 4656 4688 4719 4751 4950 4982 6646
Size: 32 125 32 31 32 199 32 1664
Cuts at: 6646 6678 6720 6752 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1664 785 631 427 267 249 223
199 153 135 125 42 32 32 32
32 32 32 32 32 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2278 5349 5595 669
Size: 988 281 340 3071 246 3079
Fragments arranged by size:
3079 3071 988 340 281 246
HindIII A'AGCT_T
Cuts at: 748 3904 748
Size: 3156 4849
HpaI GTT'AAC
Cuts at: 5595 5595
Size: 8005
KpnI G_GTAC'C
Cuts at: 5330 5330
Size: 8005
MfeI C'AATT_G
Cuts at: 5604 5604
Size: 8005
MluI A'CGCG_T
Cuts at: 7881 7881
Size: 8005
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2490 3189 3816 3843 7119 7303 1305
Size: 1185 699 627 27 3276 184 2007
Fragments arranged by size:
3276 2007 1185 699 627 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 10
Size: 54 1425 343 921 5262
Fragments arranged by size:
5262 1425 921 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 2351 3030 4865 5963 6322 6481
Size: 648 909 276 679 1835 1098 359 159
Cuts at: 6481 518
Size: 2042
Fragments arranged by size:
2042 1835 1098 909 679 648 359 276
159
MspA1I CmG'CkG
Cuts at: 1606 4473 5421 6098 7246 7491 1606
Size: 2867 948 677 1148 245 2120
Fragments arranged by size:
2867 2120 1148 948 677 245
NaeI GCC'GGC
Cuts at: 3707 3707
Size: 8005
NcoI C'CATG_G
Cuts at: 513 1065 3261 513
Size: 552 2196 5257
Fragments arranged by size:
5257 2196 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8005
NgoMIV G'CCGG_C
Cuts at: 3705 3705
Size: 8005
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8005
NotI GC'GGCC_GC
Cuts at: 5452 5452
Size: 8005
NspI r_CATG'y
Cuts at: 1132 1229 2126 2356 2522 3595 5363 6908 1132
Size: 97 897 230 166 1073 1768 1545 2229
Fragments arranged by size:
2229 1768 1545 1073 897 230 166 97
PasI CC'CwG_GG
Cuts at: 1348 2222 1348
Size: 874 7131
PciI A'CATG_T
Cuts at: 2352 2518 6904 2352
Size: 166 4386 3453
Fragments arranged by size:
4386 3453 166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2292 3267 4453 1108
Size: 1184 975 1186 4660
Fragments arranged by size:
4660 1186 1184 975
PfoI T'CCnGG_A
Cuts at: 3146 3189 3285 3428 3146
Size: 43 96 143 7723
Fragments arranged by size:
7723 143 96 43
PmeI GTTT'AAAC
Cuts at: 5309 5309
Size: 8005
PmlI CAC'GTG
Cuts at: 3781 3781
Size: 8005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6074 6106 7587 7619 6074
Size: 32 1481 32 6460
Fragments arranged by size:
6460 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3273 3391 3466 5247 3273
Size: 118 75 1781 6031
Fragments arranged by size:
6031 1781 118 75
PshAI GACnn'nnGTC
Cuts at: 1613 3325 1613
Size: 1712 6293
PsiI TTA'TAA
Cuts at: 5575 5575
Size: 8005
PspOMI G'GGCC_C
Cuts at: 2231 2231
Size: 8005
PstI C_TGCA'G
Cuts at: 830 2791 3441 3863 5357 830
Size: 1961 650 422 1494 3478
Fragments arranged by size:
3478 1961 1494 650 422
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8005
PvuII CAG'CTG
Cuts at: 4473 4473
Size: 8005
SacI G_AGCT'C
Cuts at: 5081 5190 5081
Size: 109 7896
SalI G'TCGA_C
Cuts at: 1936 2276 5347 1936
Size: 340 3071 4594
Fragments arranged by size:
4594 3071 340
SanDI GG'GwC_CC
Cuts at: 3273 3273
Size: 8005
SapI GCTCTTCn'nnn_
Cuts at: 2771 2902 2771
Size: 131 7874
SbfI CC_TGCA'GG
Cuts at: 5357 5357
Size: 8005
ScaI AGT'ACT
Cuts at: 6253 7965 6253
Size: 1712 6293
SfcI C'TryA_G
Cuts at: 826 1045 2401 2440 2709 2787 3007 3437 3859
Size: 219 1356 39 269 78 220 430 422
Cuts at: 3859 4833 5353 6488 7169 7360 826
Size: 974 520 1135 681 191 1471
Fragments arranged by size:
1471 1356 1135 974 681 520 430 422
269 220 219 191 78 39
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8005
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8005
SmaI CCC'GGG
Cuts at: 1872 3345 3512 5332 1872
Size: 1473 167 1820 4545
Fragments arranged by size:
4545 1820 1473 167
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5074 5251 6109 7010 7272 820
Size: 197 926 3131 177 858 901 262 1553
Fragments arranged by size:
3131 1553 926 901 858 262 197 177
SnaBI TAC'GTA
Cuts at: 493 4753 5796 493
Size: 4260 1043 2702
Fragments arranged by size:
4260 2702 1043
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8005
SphI G_CATG'C
Cuts at: 1132 2126 5363 1132
Size: 994 3237 3774
Fragments arranged by size:
3774 3237 994
SspI AAT'ATT
Cuts at: 5 52 5119 5929 5
Size: 47 5067 810 2081
Fragments arranged by size:
5067 2081 810 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8005
StyI C'CwwG_G
Cuts at: 513 1065 2286 2630 3246 3261 4565 5444 513
Size: 552 1221 344 616 15 1304 879 3074
Fragments arranged by size:
3074 1304 1221 879 616 552 344 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2547 2610 4383 6032 6049 6202 6387
Size: 1255 967 63 1773 1649 17 153 185
Cuts at: 6387 6832 325
Size: 445 1498
Fragments arranged by size:
1773 1649 1498 1255 967 445 185 153
63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 3360 6251
Size: 274 80 33 51 1443 411 972 2891
Cuts at: 6251 7963 96
Size: 1712 138
Fragments arranged by size:
2891 1712 1443 972 411 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 2517 2549 7965 7997 2075
Size: 32 410 32 5416 32 2083
Fragments arranged by size:
5416 2083 410 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8005
XbaI T'CTAG_A
Cuts at: 5341 5341
Size: 8005
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4487 4487
Size: 8005
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8005
XmaI C'CCGG_G
Cuts at: 1870 3343 3510 5330 1870
Size: 1473 167 1820 4545
Fragments arranged by size:
4545 1820 1473 167
XmnI GAAnn'nnTTC
Cuts at: 5118 5139 6134 5118
Size: 21 995 6989
Fragments arranged by size:
6989 995 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5813 276
Size: 53 83 186 936 4279 2468
Fragments arranged by size:
4279 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI