Restriction map for
pFN21AA0774
(Circular) (Six-base) MAPSORT of: pFN21AA0774.seq Check: 2734 from: 1 to: 8842
pFN21AA0774 8842 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2827 3278 4079 5422 2827
Size: 451 801 1343 6247
Fragments arranged by size:
6247 1343 801 451
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6652 278
Size: 53 83 186 936 5116 2468
Fragments arranged by size:
5116 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6185 1937
Size: 4248 4594
Acc65I G'GTAC_C
Cuts at: 6163 6163
Size: 8842
AclI AA'CG_TT
Cuts at: 1563 6969 7342 1563
Size: 5406 373 3063
Fragments arranged by size:
5406 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2261 4121 4144 4395 4461 4733 5057 5111
Size: 629 1860 23 251 66 272 324 54
Cuts at: 5111 5780 6905 8289 8557 1632
Size: 669 1125 1384 268 1917
Fragments arranged by size:
1917 1860 1384 1125 669 629 324 272
268 251 66 54 23
AflII C'TTAA_G
Cuts at: 820 1017 2122 4331 820
Size: 197 1105 2209 5331
Fragments arranged by size:
5331 2209 1105 197
AflIII A'CryG_T
Cuts at: 1203 2192 2827 3780 4052 7741 8718 1203
Size: 989 635 953 272 3689 977 1327
Fragments arranged by size:
3689 1327 989 977 953 635 272
AgeI A'CCGG_T
Cuts at: 7673 7673
Size: 8842
AhdI GACnn_n'nnGTC
Cuts at: 7571 7571
Size: 8842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4974 1184
Size: 3790 5052
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5261 5293 5261
Size: 32 8810
AlwNI CAG_nnn'CTG
Cuts at: 2879 3997 5332 5404 5650 6585 8157 2879
Size: 1118 1335 72 246 935 1572 3564
Fragments arranged by size:
3564 1572 1335 1118 935 246 72
ApaI G_GGCC'C
Cuts at: 6108 6108
Size: 8842
ApaLI G'TGCA_C
Cuts at: 3765 4647 6899 8055 3765
Size: 882 2252 1156 4552
Fragments arranged by size:
4552 2252 1156 882
ApoI r'AATT_y
Cuts at: 1493 2224 2264 2493 2887 3273 4127 5584 6151
Size: 731 40 229 394 386 854 1457 567
Cuts at: 6151 6377 6529 8638 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 854 731 567 394 386
229 226 152 40
AscI GG'CGCG_CC
Cuts at: 5372 5372
Size: 8842
AseI AT'TA_AT
Cuts at: 160 1032 7396 160
Size: 872 6364 1606
Fragments arranged by size:
6364 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8842
AvaI C'yCGr_G
Cuts at: 1870 1943 2640 4195 6100 6167 1870
Size: 73 697 1555 1905 67 4545
Fragments arranged by size:
4545 1905 1555 697 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4546 4579 4546
Size: 33 8809
BamHI G'GATC_C
Cuts at: 3073 3822 6172 6539 3073
Size: 749 2350 367 5376
Fragments arranged by size:
5376 2350 749 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3481 6163 7618 618
Size: 325 217 627 1694 2682 1455 1842
Fragments arranged by size:
2682 1842 1694 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3233 3492 4607 5325 5665 6108 6161 1391
Size: 1842 259 1115 718 340 443 53 4072
Fragments arranged by size:
4072 1842 1115 718 443 340 259 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2693 3829 4150 4401 5751 5895 928
Size: 971 794 1136 321 251 1350 144 3875
Fragments arranged by size:
3875 1350 1136 971 794 321 251 144
BbvCI CC'TCA_GC
Cuts at: 3601 3601
Size: 8842
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7033 7067 1903
Size: 34 5096 34 3678
Fragments arranged by size:
5096 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4547 4934 6734 7944 4547
Size: 387 1800 1210 5445
Fragments arranged by size:
5445 1800 1210 387
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8842
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4580 5009 7453
Size: 107 122 71 631 605 2908 429 2444
Cuts at: 7453 136
Size: 1525
Fragments arranged by size:
2908 2444 1525 631 605 429 122 107
71
BglII A'GATC_T
Cuts at: 5440 8837 5440
Size: 3397 5445
BlpI GC'TnA_GC
Cuts at: 5013 5405 6259 5013
Size: 392 854 7596
Fragments arranged by size:
7596 854 392
Bme1580I G_kGCm'C
Cuts at: 1790 2827 3769 4651 5164 6108 6903 8059 1790
Size: 1037 942 882 513 944 795 1156 2573
Fragments arranged by size:
2573 1156 1037 944 942 882 795 513
BmgBI CAC'GTC
Cuts at: 1318 3783 4562 1318
Size: 2465 779 5598
Fragments arranged by size:
5598 2465 779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2039 3681 3810
Size: 416 53 83 399 24 622 1642 129
Cuts at: 3810 4627 6048 6573 7531 442
Size: 817 1421 525 958 1753
Fragments arranged by size:
1753 1642 1421 958 817 622 525 416
399 129 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8842
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2185 2622 5664 2185
Size: 437 3042 5363
Fragments arranged by size:
5363 3042 437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4591 4609 4752 4762 5288 5309 5354 5729
Size: 3352 18 143 10 526 21 45 375
Cuts at: 5729 5942 7502 1239
Size: 213 1560 2579
Fragments arranged by size:
3352 2579 1560 526 375 213 143 45
21 18 10
Bpu10I CC'TnA_GC
Cuts at: 3486 3601 3486
Size: 115 8727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5704 5837 6967 7832 8130 5704
Size: 133 1130 865 298 6416
Fragments arranged by size:
6416 1130 865 298 133
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2801 4544 7505 882
Size: 617 1302 1743 2961 2219
Fragments arranged by size:
2961 2219 1743 1302 617
BsaAI yAC'GTr
Cuts at: 493 1204 6633 493
Size: 711 5429 2702
Fragments arranged by size:
5429 2702 711
BsaBI GATnn'nnATC
Cuts at: 8836 8836
Size: 8842
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4011 6649 7031 7666
Size: 53 83 186 936 2478 2638 382 635
Cuts at: 7666 275
Size: 1451
Fragments arranged by size:
2638 2478 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4166 4599 5412 7275 7673 7947 8094 8505 4166
Size: 433 813 1863 398 274 147 411 4503
Fragments arranged by size:
4503 1863 813 433 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5261 5291 6077 6107 1178
Size: 30 165 30 3858 30 786 30 3913
Fragments arranged by size:
3913 3858 786 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2566 3195 3410 3920 4266 4606 5276
Size: 975 405 629 215 510 346 340 670
Cuts at: 5276 5351 5399 5714 6085 6088 6100 1186
Size: 75 48 315 371 3 12 3928
Fragments arranged by size:
3928 975 670 629 510 405 371 346
340 315 215 75 48 12 3
BseYI C'CCAG_C
Cuts at: 4338 5068 5299 8045 8541 4338
Size: 730 231 2746 496 4639
Fragments arranged by size:
4639 2746 730 496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2863 3306 1163
Size: 573 1127 443 6699
Fragments arranged by size:
6699 1127 573 443
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4756 6292 7053 8081 1540
Size: 275 186 2755 1536 761 1028 2301
Fragments arranged by size:
2755 2301 1536 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 2798 2853 3769 4607 4651 5325 5391 6903
Size: 428 55 916 838 44 674 66 1512
Cuts at: 6903 6988 8059 2370
Size: 85 1071 3153
Fragments arranged by size:
3153 1512 1071 916 838 674 428 85
66 55 44
BsmI GAATG_Cn'
Cuts at: 2146 2557 3583 6353 6446 2146
Size: 411 1026 2770 93 4542
Fragments arranged by size:
4542 2770 1026 411 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4568 4618 4568
Size: 50 8792
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2370 2798 2827 2853 3233 3492 3769
Size: 399 580 428 29 26 380 259 277
Cuts at: 3769 4607 4651 5164 5325 5391 5665 6108 6161
Size: 838 44 513 161 66 274 443 53
Cuts at: 6161 6903 6988 8059 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 838 742 580 513 443 428
399 380 277 274 259 161 85 66
53 44 29 26
BspEI T'CCGG_A
Cuts at: 4166 4599 8505 4166
Size: 433 3906 4503
Fragments arranged by size:
4503 3906 433
BspHI T'CATG_A
Cuts at: 2176 5554 6731 8461 2176
Size: 3378 1177 1730 2557
Fragments arranged by size:
3378 2557 1730 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2827 3278 4079 4149 4891 5422 5755 6197
Size: 1983 451 801 70 742 531 333 442
Cuts at: 6197 844
Size: 3489
Fragments arranged by size:
3489 1983 801 742 531 451 442 333
70
BsrBI CCG'CTC
Cuts at: 1394 1823 3513 5017 5044 5341 6729 1394
Size: 429 1690 1504 27 297 1388 3507
Fragments arranged by size:
3507 1690 1504 1388 429 297 27
BsrDI GCAATG_nn'
Cuts at: 65 1248 2690 4362 5606 7337 7511 65
Size: 1183 1442 1672 1244 1731 174 1396
Fragments arranged by size:
1731 1672 1442 1396 1244 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4665 4751 7486 7673 1700
Size: 110 2855 86 2735 187 2869
Fragments arranged by size:
2869 2855 2735 187 110 86
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8842
BssHII G'CGCG_C
Cuts at: 1925 5372 1925
Size: 3447 5395
BssSI C'ACGA_G
Cuts at: 5795 6902 7914 5795
Size: 1107 1012 6723
Fragments arranged by size:
6723 1107 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5026 794
Size: 4232 4610
BstBI TT'CG_AA
Cuts at: 5673 7646 5673
Size: 1973 6869
BstEII G'GTnAC_C
Cuts at: 1184 3538 4094 5393 1184
Size: 2354 556 1299 4633
Fragments arranged by size:
4633 2354 1299 556
BstXI CCAn_nnnn'nTGG
Cuts at: 3146 5068 3146
Size: 1922 6920
BstYI r'GATC_y
Cuts at: 1972 2965 3073 3822 5440 5862 6172 6539 6924
Size: 993 108 749 1618 422 310 367 385
Cuts at: 6924 6941 8393 8479 8491 8837 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1618 1452 993 749 422 385 367
346 310 108 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3241 3241
Size: 8842
BtgI C'CryG_G
Cuts at: 513 1065 2004 5124 6088 513
Size: 552 939 3120 964 3267
Fragments arranged by size:
3267 3120 964 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8103
Fragments arranged by size:
8103 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3421 6358 7152 7172 2851
Size: 570 2937 794 20 4521
Fragments arranged by size:
4521 2937 794 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3532 3567 529
Size: 35 2968 35 5804
Fragments arranged by size:
5804 2968 35 35
DraI TTT'AAA
Cuts at: 4488 6146 4488
Size: 1658 7184
DraIII CAC_nnn'GTG
Cuts at: 2374 3284 3332 2374
Size: 910 48 7884
Fragments arranged by size:
7884 910 48
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2705 5531 7849 809
Size: 507 1389 2826 2318 1802
Fragments arranged by size:
2826 2318 1802 1389 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3115 3508 4667
Size: 54 1425 50 275 18 1285 393 1159
Cuts at: 4667 4753 5127 6117 6289 7178 8807 8
Size: 86 374 990 172 889 1629 43
Fragments arranged by size:
1629 1425 1285 1159 990 889 393 374
275 172 86 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4753 6289 1537
Size: 275 2941 1536 4090
Fragments arranged by size:
4090 2941 1536 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 3039 3151 3165 3170 4071 4257 5087
Size: 1005 681 112 14 5 901 186 830
Cuts at: 5087 5288 5670 5747 5987 6009 6772 1353
Size: 201 382 77 240 22 763 3423
Fragments arranged by size:
3423 1005 901 830 763 681 382 240
201 186 112 77 22 14 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2854 4971 5378 7429 7803 7949
Size: 1064 138 1265 2117 407 2051 374 146
Cuts at: 7949 387
Size: 1280
Fragments arranged by size:
2117 2051 1280 1265 1064 407 374 146
138
EcoICRI GAG'CTC
Cuts at: 4605 5323 4605
Size: 718 8124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 4121 4144 4395 4461 4591 4609
Size: 393 629 1860 23 251 66 130 18
Cuts at: 4609 4733 4752 4762 5057 5111 5288 5309 5354
Size: 124 19 10 295 54 177 21 45
Cuts at: 5354 5729 5780 5942 6905 7502 8289 8557 1239
Size: 375 51 162 963 597 787 268 1524
Fragments arranged by size:
1860 1524 963 787 629 597 393 375
295 268 251 177 162 130 124 66
54 51 45 23 21 19 18 10
EcoNI CCTnn'n_nnAGG
Cuts at: 3976 4001 5381 3976
Size: 25 1380 7437
Fragments arranged by size:
7437 1380 25
EcoO109I rG'GnC_Cy
Cuts at: 3732 3994 4436 6043 6104 6105 3732
Size: 262 442 1607 61 1 6469
Fragments arranged by size:
6469 1607 442 262 61 1
EcoRI G'AATT_C
Cuts at: 6151 6529 6151
Size: 378 8464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3853 6038 3853
Size: 2185 6657
FseI GG_CCGG'CC
Cuts at: 4669 4755 4669
Size: 86 8756
FspI TGC'GCA
Cuts at: 5079 7348 5079
Size: 2269 6573
HaeII r_GCGC'y
Cuts at: 1678 4422 4817 7989 1678
Size: 2744 395 3172 2531
Fragments arranged by size:
3172 2744 2531 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2743 2755 2775 2840
Size: 32 135 32 1151 20 12 20 65
Cuts at: 2840 2872 3038 3070 3716 3748 4384 4416 5536
Size: 32 166 32 646 32 636 32 1120
Cuts at: 5536 5568 7483 7515 7557 7589 1373
Size: 32 1915 32 42 32 2626
Fragments arranged by size:
2626 1915 1151 1120 646 636 166 135
65 42 32 32 32 32 32 32
32 32 32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 3187 5420 6186 6432 669
Size: 988 281 93 1156 2233 766 246 3079
Fragments arranged by size:
3079 2233 1156 988 766 281 246 93
HindIII A'AGCT_T
Cuts at: 748 4494 4925 748
Size: 3746 431 4665
Fragments arranged by size:
4665 3746 431
HpaI GTT'AAC
Cuts at: 6432 6432
Size: 8842
KpnI G_GTAC'C
Cuts at: 6167 6167
Size: 8842
MfeI C'AATT_G
Cuts at: 6441 6441
Size: 8842
MluI A'CGCG_T
Cuts at: 8718 8718
Size: 8842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 2417 3442 4328 5899 5919 7956 8140
Size: 834 278 1025 886 1571 20 2037 184
Cuts at: 8140 1305
Size: 2007
Fragments arranged by size:
2037 2007 1571 1025 886 834 278 184
20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3117 5129 10
Size: 54 1425 343 1285 2012 3723
Fragments arranged by size:
3723 2012 1425 1285 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3932 6800 7159 7318 518
Size: 648 2766 2868 359 159 2042
Fragments arranged by size:
2868 2766 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4838 5023 5068 5350 5401 5707 5935 6090
Size: 3232 185 45 282 51 306 228 155
Cuts at: 6090 6258 6935 8083 8328 1606
Size: 168 677 1148 245 2120
Fragments arranged by size:
3232 2120 1148 677 306 282 245 228
185 168 155 51 45
NaeI GCC'GGC
Cuts at: 4667 4753 4667
Size: 86 8756
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7351
Fragments arranged by size:
7351 939 552
NdeI CA'TA_TG
Cuts at: 387 4039 387
Size: 3652 5190
NgoMIV G'CCGG_C
Cuts at: 4665 4751 4665
Size: 86 8756
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8842
NotI GC'GGCC_GC
Cuts at: 6289 6289
Size: 8842
NspI r_CATG'y
Cuts at: 1132 1229 2196 2507 2831 3953 4056 6200 7745
Size: 97 967 311 324 1122 103 2144 1545
Cuts at: 7745 1132
Size: 2229
Fragments arranged by size:
2229 2144 1545 1122 967 324 311 103
97
PasI CC'CwG_GG
Cuts at: 1348 3191 3997 1348
Size: 1843 806 6193
Fragments arranged by size:
6193 1843 806
PciI A'CATG_T
Cuts at: 2192 2827 4052 7741 2192
Size: 635 1225 3689 3293
Fragments arranged by size:
3689 3293 1225 635
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2818 3997 5332 1108
Size: 1710 1179 1335 4618
Fragments arranged by size:
4618 1710 1335 1179
PfoI T'CCnGG_A
Cuts at: 4549 5325 5958 4549
Size: 776 633 7433
Fragments arranged by size:
7433 776 633
PmeI GTTT'AAAC
Cuts at: 6146 6146
Size: 8842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5261 5293 6911 6943 8424 8456 5261
Size: 32 1618 32 1481 32 5647
Fragments arranged by size:
5647 1618 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3732 4436 6043 3732
Size: 704 1607 6531
Fragments arranged by size:
6531 1607 704
PshAI GACnn'nnGTC
Cuts at: 1613 5935 1613
Size: 4322 4520
PsiI TTA'TAA
Cuts at: 6412 6412
Size: 8842
PspOMI G'GGCC_C
Cuts at: 6104 6104
Size: 8842
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 8810
PstI C_TGCA'G
Cuts at: 830 2400 2593 2838 4146 5349 5382 5514 6194
Size: 1570 193 245 1308 1203 33 132 680
Cuts at: 6194 830
Size: 3478
Fragments arranged by size:
3478 1570 1308 1203 680 245 193 132
33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8842
PvuII CAG'CTG
Cuts at: 5023 5068 5350 5401 5707 5935 5023
Size: 45 282 51 306 228 7930
Fragments arranged by size:
7930 306 282 228 51 45
SacI G_AGCT'C
Cuts at: 4607 5325 4607
Size: 718 8124
SacII CC_GC'GG
Cuts at: 6091 6091
Size: 8842
SalI G'TCGA_C
Cuts at: 1936 6184 1936
Size: 4248 4594
SanDI GG'GwC_CC
Cuts at: 3732 6043 3732
Size: 2311 6531
SapI GCTCTTCn'nnn_
Cuts at: 2358 6009 2358
Size: 3651 5191
SbfI CC_TGCA'GG
Cuts at: 5382 5514 6194 5382
Size: 132 680 8030
Fragments arranged by size:
8030 680 132
ScaI AGT'ACT
Cuts at: 7090 8802 7090
Size: 1712 7130
SexAI A'CCwGG_T
Cuts at: 3328 5616 3328
Size: 2288 6554
SfcI C'TryA_G
Cuts at: 826 1045 2396 2583 2589 2834 4142 4462 5345
Size: 219 1351 187 6 245 1308 320 883
Cuts at: 5345 5378 5510 6190 7325 8006 8197 826
Size: 33 132 680 1135 681 191 1471
Fragments arranged by size:
1471 1351 1308 1135 883 681 680 320
245 219 191 187 132 33 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8842
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8842
SmaI CCC'GGG
Cuts at: 1872 2642 4197 6169 1872
Size: 770 1555 1972 4545
Fragments arranged by size:
4545 1972 1555 770
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2122 4331 5683 5816 6946 7847
Size: 197 926 179 2209 1352 133 1130 901
Cuts at: 7847 8109 820
Size: 262 1553
Fragments arranged by size:
2209 1553 1352 1130 926 901 262 197
179 133
SnaBI TAC'GTA
Cuts at: 493 6633 493
Size: 6140 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8842
SphI G_CATG'C
Cuts at: 1132 6200 1132
Size: 5068 3774
SspI AAT'ATT
Cuts at: 5 52 3628 6766 5
Size: 47 3576 3138 2081
Fragments arranged by size:
3576 3138 2081 47
StuI AGG'CCT
Cuts at: 1517 5681 1517
Size: 4164 4678
StyI C'CwwG_G
Cuts at: 513 1065 2004 2511 3060 3145 3381 3738 3849
Size: 552 939 507 549 85 236 357 111
Cuts at: 3849 4151 4743 6281 513
Size: 302 592 1538 3074
Fragments arranged by size:
3074 1538 939 592 552 549 507 357
302 236 111 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3290 3660 3700 4305 4340 4443 4646
Size: 1255 1710 370 40 605 35 103 203
Cuts at: 4646 4756 6151 6869 6886 7039 7224 7669 325
Size: 110 1395 718 17 153 185 445 1498
Fragments arranged by size:
1710 1498 1395 1255 718 605 445 370
203 185 153 110 103 40 35 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2830 7088 8800
Size: 274 80 33 51 1443 853 4258 1712
Cuts at: 8800 96
Size: 138
Fragments arranged by size:
4258 1712 1443 853 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2739 2771 4161 4193 8802 8834 2739
Size: 32 1390 32 4609 32 2747
Fragments arranged by size:
4609 2747 1390 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2424 1316
Size: 1108 7734
XbaI T'CTAG_A
Cuts at: 2464 3571 6178 2464
Size: 1107 2607 5128
Fragments arranged by size:
5128 2607 1107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2518 2518
Size: 8842
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8842
XmaI C'CCGG_G
Cuts at: 1870 2640 4195 6167 1870
Size: 770 1555 1972 4545
Fragments arranged by size:
4545 1972 1555 770
XmnI GAAnn'nnTTC
Cuts at: 6971 6971
Size: 8842
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6650 276
Size: 53 83 186 936 5116 2468
Fragments arranged by size:
5116 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AvrII BbeI BfrBI BsiWI BstZ17I EcoRV FspAI
KasI NarI NruI NsiI PacI PmlI PspXI RsrII
SfiI SfoI SrfI SwaI