Restriction map for
pFN21AA0773
(Circular) (Six-base) MAPSORT of: pFN21AA0773.seq Check: 4376 from: 1 to: 5785
pFN21AA0773 5785 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3033 3595 278
Size: 53 83 186 936 1497 562 2468
Fragments arranged by size:
2468 1497 936 562 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 3128 1937
Size: 1065 126 4594
Fragments arranged by size:
4594 1065 126
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3084 3912 4285 1563
Size: 1521 828 373 3063
Fragments arranged by size:
3063 1521 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2796 3848 5232 5500 1632
Size: 455 709 1052 1384 268 1917
Fragments arranged by size:
1917 1384 1052 709 455 268
AflII C'TTAA_G
Cuts at: 820 1017 2216 820
Size: 197 1199 4389
Fragments arranged by size:
4389 1199 197
AflIII A'CryG_T
Cuts at: 1203 2658 4684 5661 1203
Size: 1455 2026 977 1327
Fragments arranged by size:
2026 1455 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 2968 4514 2968
Size: 1546 4239
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2599 3528 5100 2599
Size: 929 1572 3284
Fragments arranged by size:
3284 1572 929
ApaI G_GGCC'C
Cuts at: 2251 2903 2251
Size: 652 5133
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 3094 3320 3472 5581 1493
Size: 1601 226 152 2109 1697
Fragments arranged by size:
2109 1697 1601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 2741 3115 3482 2741
Size: 374 367 5044
Fragments arranged by size:
5044 374 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2929 3106 4561 618
Size: 325 217 627 1142 177 1455 1842
Fragments arranged by size:
1842 1455 1142 627 325 217 177
BanII G_rGCy'C
Cuts at: 1391 2251 2869 2903 3104 1391
Size: 860 618 34 201 4072
Fragments arranged by size:
4072 860 618 201 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2372 2511 2994 928
Size: 971 473 139 483 3719
Fragments arranged by size:
3719 971 483 473 139
BbvCI CC'TCA_GC
Cuts at: 2435 2907 2435
Size: 472 5313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3976 4010 1903
Size: 34 2039 34 3678
Fragments arranged by size:
3678 2039 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2407 2810 3677 4887 2407
Size: 403 867 1210 3305
Fragments arranged by size:
3305 1210 867 403
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2732 4396 136
Size: 107 122 71 631 605 1060 1664 1525
Fragments arranged by size:
1664 1525 1060 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2251 2903 3846 5002 1790
Size: 461 652 943 1156 2573
Fragments arranged by size:
2573 1156 943 652 461
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2872 3516 4474
Size: 416 53 83 399 24 1455 644 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1455 958 644 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2849 2987 4445 1239
Size: 1610 138 1458 2579
Fragments arranged by size:
2579 1610 1458 138
Bpu10I CC'TnA_GC
Cuts at: 2435 2907 2435
Size: 472 5313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2610 3910 4775 5073 2610
Size: 1300 865 298 3322
Fragments arranged by size:
3322 1300 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2659 3576 493
Size: 711 1455 917 2702
Fragments arranged by size:
2702 1455 917 711
BsaBI GATnn'nnATC
Cuts at: 2076 5779 2076
Size: 3703 2082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2472 3030 3592 3974
Size: 53 83 186 936 939 558 562 382
Cuts at: 3974 4609 275
Size: 635 1451
Fragments arranged by size:
1451 939 936 635 562 558 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2924 2927 2951 3077 1186
Size: 1738 3 24 126 3894
Fragments arranged by size:
3894 1738 126 24 3
BseYI C'CCAG_C
Cuts at: 2358 2777 2845 4988 5484 2358
Size: 419 68 2143 496 2659
Fragments arranged by size:
2659 2143 496 419 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 3068 1163
Size: 573 357 975 3880
Fragments arranged by size:
3880 975 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2251 2869 2903 3104 3846 3931 5002
Size: 399 461 618 34 201 742 85 1071
Cuts at: 5002 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 618 461 399 201 85
34
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3140 844
Size: 2296 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3008 3672 1394
Size: 429 1185 664 3507
Fragments arranged by size:
3507 1185 664 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2721 4280 4454 65
Size: 1183 1473 1559 174 1396
Fragments arranged by size:
1559 1473 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 2982 3845 4857 2982
Size: 863 1012 3910
Fragments arranged by size:
3910 1012 863
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 2509 2317
Size: 192 5593
BstYI r'GATC_y
Cuts at: 1972 2274 2741 3115 3482 3867 3884 5336 5422
Size: 302 467 374 367 385 17 1452 86
Cuts at: 5422 5434 5780 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 467 385 374 367 346 302
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2934 3040 2934
Size: 106 5679
BtgI C'CryG_G
Cuts at: 513 1065 2004 2184 2316 2523 2634 513
Size: 552 939 180 132 207 111 3664
Fragments arranged by size:
3664 939 552 207 180 132 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2454 487
Size: 683 56 1228 3818
Fragments arranged by size:
3818 1228 683 56
BtsI GCAGTG_nn'
Cuts at: 2055 2105 2889 3301 4095 4115 2055
Size: 50 784 412 794 20 3725
Fragments arranged by size:
3725 794 784 412 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2129 2253 2733
Size: 54 1425 50 275 18 299 124 480
Cuts at: 2733 3232 4121 5750 8
Size: 499 889 1629 43
Fragments arranged by size:
1629 1425 889 499 480 299 275 124
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2912 2937 3715 1353
Size: 1559 25 778 3423
Fragments arranged by size:
3423 1559 778 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4372 4746 4892 387
Size: 1064 138 2783 374 146 1280
Fragments arranged by size:
2783 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2796 2849 2987 3848 4445 5232
Size: 393 455 709 53 138 861 597 787
Cuts at: 5232 5500 1239
Size: 268 1524
Fragments arranged by size:
1524 861 787 709 597 455 393 268
138 53
EcoO109I rG'GnC_Cy
Cuts at: 2062 2247 2248 2795 2899 2900 2962 2062
Size: 185 1 547 104 1 62 4885
Fragments arranged by size:
4885 547 185 104 62 1 1
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 2408 4291 2408
Size: 1883 3902
HaeII r_GCGC'y
Cuts at: 1678 4932 1678
Size: 3254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2593 2625 2954
Size: 32 135 32 839 32 150 32 329
Cuts at: 2954 2986 3020 3052 4426 4458 4500 4532 1373
Size: 32 34 32 1374 32 42 32 2626
Fragments arranged by size:
2626 1374 839 329 150 135 42 34
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3129 3375 669
Size: 988 281 1191 246 3079
Fragments arranged by size:
3079 1191 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2213 748
Size: 1465 4320
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 4899 5083 1305
Size: 1149 2445 184 2007
Fragments arranged by size:
2445 2007 1149 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 3540
Fragments arranged by size:
3540 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 2394 2507 2543 3743 4102 4261
Size: 648 1149 79 113 36 1200 359 159
Cuts at: 4261 518
Size: 2042
Fragments arranged by size:
2042 1200 1149 648 359 159 113 79
36
MspA1I CmG'CkG
Cuts at: 1606 2375 3201 3878 5026 5271 1606
Size: 769 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 769 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2184 2316 2523 2634 513
Size: 552 939 180 132 207 111 3664
Fragments arranged by size:
3664 939 552 207 180 132 111
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2665 1348
Size: 1317 4468
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PfoI T'CCnGG_A
Cuts at: 2021 2021
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2795 2962 2795
Size: 167 5618
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PspOMI G'GGCC_C
Cuts at: 2247 2899 2247
Size: 652 5133
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2375 2375
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SfcI C'TryA_G
Cuts at: 826 1045 2578 3133 4268 4949 5140 826
Size: 219 1533 555 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 555 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 2625 3889 4790 5052 820
Size: 197 926 273 409 1264 901 262 1553
Fragments arranged by size:
1553 1264 926 901 409 273 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2184 2316 2523 2634 3224 513
Size: 552 939 180 132 207 111 590 3074
Fragments arranged by size:
3074 939 590 552 207 180 132 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2570 2647 3812 3829 3982 4167 4612
Size: 1255 990 77 1165 17 153 185 445
Cuts at: 4612 325
Size: 1498
Fragments arranged by size:
1498 1255 1165 990 445 185 153 77
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 4031 5743
Size: 274 80 33 51 1443 696 1358 1712
Cuts at: 5743 96
Size: 138
Fragments arranged by size:
1712 1443 1358 696 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 3121 3121
Size: 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3031 3593 276
Size: 53 83 186 936 1497 562 2468
Fragments arranged by size:
2468 1497 936 562 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI