Restriction map for
pFN21AA0767
(Circular) (Six-base) MAPSORT of: pFN21AA0767.seq Check: 8489 from: 1 to: 6439
pFN21AA0767 6439 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2919 4249 278
Size: 53 83 186 936 1383 1330 2468
Fragments arranged by size:
2468 1383 1330 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2697 3617 3782 1937
Size: 760 920 165 4594
Fragments arranged by size:
4594 920 760 165
Acc65I G'GTAC_C
Cuts at: 2122 3760 2122
Size: 1638 4801
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2420 4502 5886 6154 1632
Size: 506 282 2082 1384 268 1917
Fragments arranged by size:
2082 1917 1384 506 282 268
AfeI AGC'GCT
Cuts at: 3218 3218
Size: 6439
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 2700 5338 6315 1203
Size: 1497 2638 977 1327
Fragments arranged by size:
2638 1497 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 5168 5168
Size: 6439
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 6407
AlwNI CAG_nnn'CTG
Cuts at: 2344 2488 3016 3725 4182 5754 2344
Size: 144 528 709 457 1572 3029
Fragments arranged by size:
3029 1572 709 528 457 144
ApaLI G'TGCA_C
Cuts at: 3659 4496 5652 3659
Size: 837 1156 4446
Fragments arranged by size:
4446 1156 837
ApoI r'AATT_y
Cuts at: 1493 3748 3974 4126 6235 1493
Size: 2255 226 152 2109 1697
Fragments arranged by size:
2255 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2558 3142 3764 1870
Size: 73 196 419 584 622 4545
Fragments arranged by size:
4545 622 584 419 196 73
BamHI G'GATC_C
Cuts at: 3769 4136 3769
Size: 367 6072
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2636 3650 3760 5215
Size: 325 217 627 335 514 1014 110 1455
Cuts at: 5215 618
Size: 1842
Fragments arranged by size:
1842 1455 1014 627 514 335 325 217
110
BanII G_rGCy'C
Cuts at: 1391 2298 2859 3758 1391
Size: 907 561 899 4072
Fragments arranged by size:
4072 907 899 561
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2883 928
Size: 971 984 4484
Fragments arranged by size:
4484 984 971
BbvCI CC'TCA_GC
Cuts at: 2156 3712 2156
Size: 1556 4883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4630 4664 1903
Size: 34 50 34 2609 34 3678
Fragments arranged by size:
3678 2609 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2817 4331 5541 2817
Size: 1514 1210 3715
Fragments arranged by size:
3715 1514 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3044 5050 136
Size: 107 122 71 631 605 1372 2006 1525
Fragments arranged by size:
2006 1525 1372 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3351 6434 3351
Size: 3083 3356
BlpI GC'TnA_GC
Cuts at: 2427 3856 2427
Size: 1429 5010
Bme1580I G_kGCm'C
Cuts at: 1790 2844 3663 4500 5656 1790
Size: 1054 819 837 1156 2573
Fragments arranged by size:
2573 1156 1054 837 819
BmgBI CAC'GTC
Cuts at: 1318 2703 1318
Size: 1385 5054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4170 5128 442
Size: 416 53 83 399 24 2753 958 1753
Fragments arranged by size:
2753 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2396 2984 3202 3205 5099 1239
Size: 1157 588 218 3 1894 2579
Fragments arranged by size:
2579 1894 1157 588 218 3
Bpu10I CC'TnA_GC
Cuts at: 2009 2156 2591 3595 3712 2009
Size: 147 435 1004 117 4736
Fragments arranged by size:
4736 1004 435 147 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 3194 4564 5429 5727 2612
Size: 582 1370 865 298 3324
Fragments arranged by size:
3324 1370 865 582 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3674 5102 882
Size: 617 2175 1428 2219
Fragments arranged by size:
2219 2175 1428 617
BsaAI yAC'GTr
Cuts at: 493 1204 2536 3271 4230 493
Size: 711 1332 735 959 2702
Fragments arranged by size:
2702 1332 959 735 711
BsaBI GATnn'nnATC
Cuts at: 6433 6433
Size: 6439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2916 3309 3620 4246
Size: 53 83 186 936 1383 393 311 626
Cuts at: 4246 4628 5263 275
Size: 382 635 1451
Fragments arranged by size:
1451 1383 936 635 626 393 382 311
186 83 53
BsaWI w'CCGG_w
Cuts at: 2214 2298 2547 3429 4872 5270 5544 5691 6102
Size: 84 249 882 1443 398 274 147 411
Cuts at: 6102 2214
Size: 2551
Fragments arranged by size:
2551 1443 882 411 398 274 249 147
84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 1178
Size: 30 165 30 1272 30 4912
Fragments arranged by size:
4912 1272 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2165 2306 2429 2576 3199 3317 1186
Size: 979 141 123 147 623 118 4308
Fragments arranged by size:
4308 979 623 147 141 123 118
BseYI C'CCAG_C
Cuts at: 2748 3275 5642 6138 2748
Size: 527 2367 496 3049
Fragments arranged by size:
3049 2367 527 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 1163
Size: 573 672 5194
Fragments arranged by size:
5194 672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3288 3316 3889 4650 5678 1540
Size: 275 186 1287 28 573 761 1028 2301
Fragments arranged by size:
2301 1287 1028 761 573 275 186 28
BsiHKAI G_wGCw'C
Cuts at: 2298 3468 3663 4500 4585 5656 2298
Size: 1170 195 837 85 1071 3081
Fragments arranged by size:
3081 1170 1071 837 195 85
BsmI GAATG_Cn'
Cuts at: 2102 2737 3950 4043 2102
Size: 635 1213 93 4498
Fragments arranged by size:
4498 1213 635 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2614 2614
Size: 6439
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2844 2859 3468 3663 3758 4500
Size: 399 508 546 15 609 195 95 742
Cuts at: 4500 4585 5656 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 609 546 508 399 195
95 85 15
BspEI T'CCGG_A
Cuts at: 2298 6102 2298
Size: 3804 2635
BspHI T'CATG_A
Cuts at: 4328 6058 4328
Size: 1730 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2592 3794 844
Size: 1748 1202 3489
Fragments arranged by size:
3489 1748 1202
BsrBI CCG'CTC
Cuts at: 1394 1823 2356 2521 3640 4326 1394
Size: 429 533 165 1119 686 3507
Fragments arranged by size:
3507 1119 686 533 429 165
BsrDI GCAATG_nn'
Cuts at: 65 1248 2467 2709 4934 5108 65
Size: 1183 1219 242 2225 174 1396
Fragments arranged by size:
2225 1396 1219 1183 242 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2644 3283 3287 3311 5083 5270 1700
Size: 110 834 639 4 24 1772 187 2869
Fragments arranged by size:
2869 1772 834 639 187 110 24 4
BsrGI T'GTAC_A
Cuts at: 96 2724 96
Size: 2628 3811
BssHII G'CGCG_C
Cuts at: 1925 3151 1925
Size: 1226 5213
BssSI C'ACGA_G
Cuts at: 3347 3641 4499 5511 3347
Size: 294 858 1012 4275
Fragments arranged by size:
4275 1012 858 294
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2386 2395 794
Size: 1592 9 4838
Fragments arranged by size:
4838 1592 9
BstBI TT'CG_AA
Cuts at: 3495 5243 3495
Size: 1748 4691
BstEII G'GTnAC_C
Cuts at: 1184 2249 1184
Size: 1065 5374
BstYI r'GATC_y
Cuts at: 1972 3351 3769 4136 4521 4538 5990 6076 6088
Size: 1379 418 367 385 17 1452 86 12
Cuts at: 6088 6434 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1379 418 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2126 2859 3084 3441 3654 513
Size: 552 1061 733 225 357 213 3298
Fragments arranged by size:
3298 1061 733 552 357 225 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2791 487
Size: 683 56 1565 4135
Fragments arranged by size:
4135 1565 683 56
BtsI GCAGTG_nn'
Cuts at: 3955 4749 4769 3955
Size: 794 20 5625
Fragments arranged by size:
5625 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6404
DraI TTT'AAA
Cuts at: 3133 3743 3133
Size: 610 5829
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3256 5446 809
Size: 507 1940 2190 1802
Fragments arranged by size:
2190 1940 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2190 3285 3886
Size: 54 1425 50 275 18 360 1095 601
Cuts at: 3886 4775 6404 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1095 889 601 360 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3285 3886 1537
Size: 275 1473 601 4090
Fragments arranged by size:
4090 1473 601 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4369 1353
Size: 3016 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2380 2836 5026 5400 5546 387
Size: 1064 138 791 456 2190 374 146 1280
Fragments arranged by size:
2190 1280 1064 791 456 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 6439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2396 2420 2984 3202 3205 4502
Size: 393 506 258 24 564 218 3 1297
Cuts at: 4502 5099 5886 6154 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1297 787 597 564 506 393 268
258 218 24 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3520 3520
Size: 6439
EcoO109I rG'GnC_Cy
Cuts at: 2161 2178 3513 3574 2161
Size: 17 1335 61 5026
Fragments arranged by size:
5026 1335 61 17
EcoRI G'AATT_C
Cuts at: 3748 4126 3748
Size: 378 6061
FseI GG_CCGG'CC
Cuts at: 3291 3291
Size: 6439
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 2476 2598 3029 3220 3228 3409 5586 1678
Size: 798 122 431 191 8 181 2177 2531
Fragments arranged by size:
2531 2177 798 431 191 181 122 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2233 2265 2675 2707 3343
Size: 32 135 32 661 32 410 32 636
Cuts at: 3343 3375 5080 5112 5154 5186 1373
Size: 32 1705 32 42 32 2626
Fragments arranged by size:
2626 1705 661 636 410 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2607 3618 3783 4029 669
Size: 988 281 669 1011 165 246 3079
Fragments arranged by size:
3079 1011 988 669 281 246 165
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6439
HpaI GTT'AAC
Cuts at: 2607 4029 2607
Size: 1422 5017
KpnI G_GTAC'C
Cuts at: 2126 3764 2126
Size: 1638 4801
MfeI C'AATT_G
Cuts at: 4038 4038
Size: 6439
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1998 2706 5553 5737 1305
Size: 693 708 2847 184 2007
Fragments arranged by size:
2847 2007 708 693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2192 10
Size: 54 1425 343 360 4257
Fragments arranged by size:
4257 1425 360 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3666 4397 4756 4915 518
Size: 648 2500 731 359 159 2042
Fragments arranged by size:
2500 2042 731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2362 2905 3382 3716 3855 4532 5680
Size: 522 234 543 477 334 139 677 1148
Cuts at: 5680 5925 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 543 522 477 334 245
234 139
NaeI GCC'GGC
Cuts at: 3289 3289
Size: 6439
NcoI C'CATG_G
Cuts at: 513 1065 3654 513
Size: 552 2589 3298
Fragments arranged by size:
3298 2589 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6439
NgoMIV G'CCGG_C
Cuts at: 3287 3287
Size: 6439
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 3886 3886
Size: 6439
NspI r_CATG'y
Cuts at: 1132 1229 3797 5342 1132
Size: 97 2568 1545 2229
Fragments arranged by size:
2568 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6439
PciI A'CATG_T
Cuts at: 5338 5338
Size: 6439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2817 1108
Size: 1709 4730
PfoI T'CCnGG_A
Cuts at: 2202 2961 2202
Size: 759 5680
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4508 4540 6021 6053 4508
Size: 32 1481 32 4894
Fragments arranged by size:
4894 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2161 2178 3513 3574 2161
Size: 17 1335 61 5026
Fragments arranged by size:
5026 1335 61 17
PshAI GACnn'nnGTC
Cuts at: 1613 3312 1613
Size: 1699 4740
PsiI TTA'TAA
Cuts at: 4009 4009
Size: 6439
PstI C_TGCA'G
Cuts at: 830 2505 3249 3791 830
Size: 1675 744 542 3478
Fragments arranged by size:
3478 1675 744 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
PvuII CAG'CTG
Cuts at: 2905 2905
Size: 6439
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 6439
SacII CC_GC'GG
Cuts at: 2129 2129
Size: 6439
SalI G'TCGA_C
Cuts at: 1936 3616 3781 1936
Size: 1680 165 4594
Fragments arranged by size:
4594 1680 165
SanDI GG'GwC_CC
Cuts at: 2161 2178 2161
Size: 17 6422
SbfI CC_TGCA'GG
Cuts at: 3791 3791
Size: 6439
ScaI AGT'ACT
Cuts at: 4687 6399 4687
Size: 1712 4727
SfcI C'TryA_G
Cuts at: 826 1045 2501 3245 3787 4922 5603 5794 826
Size: 219 1456 744 542 1135 681 191 1471
Fragments arranged by size:
1471 1456 1135 744 681 542 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 2141 3766 1872
Size: 269 1625 4545
Fragments arranged by size:
4545 1625 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 3209 4543 5444 5706 820
Size: 197 926 684 582 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 684 582 262 197
SnaBI TAC'GTA
Cuts at: 493 3271 4230 493
Size: 2778 959 2702
Fragments arranged by size:
2778 2702 959
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 3797 1132
Size: 2665 3774
SspI AAT'ATT
Cuts at: 5 52 4363 5
Size: 47 4311 2081
Fragments arranged by size:
4311 2081 47
StuI AGG'CCT
Cuts at: 1517 3674 1517
Size: 2157 4282
StyI C'CwwG_G
Cuts at: 513 1065 3654 3878 513
Size: 552 2589 224 3074
Fragments arranged by size:
3074 2589 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3644 4466 4483 4636 4821 5266 325
Size: 1255 2064 822 17 153 185 445 1498
Fragments arranged by size:
2064 1498 1255 822 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 3567 3696
Size: 274 80 33 51 1443 747 843 129
Cuts at: 3696 4685 6397 96
Size: 989 1712 138
Fragments arranged by size:
1712 1443 989 843 747 274 138 129
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6399 6431 6399
Size: 32 6407
Tth111I GACn'n_nGTC
Cuts at: 1316 2887 3256 1316
Size: 1571 369 4499
Fragments arranged by size:
4499 1571 369
XbaI T'CTAG_A
Cuts at: 3775 3775
Size: 6439
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2459 2459
Size: 6439
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6439
XmaI C'CCGG_G
Cuts at: 1870 2139 3764 1870
Size: 269 1625 4545
Fragments arranged by size:
4545 1625 269
XmnI GAAnn'nnTTC
Cuts at: 2510 2939 2981 4568 2510
Size: 429 42 1587 4381
Fragments arranged by size:
4381 1587 429 42
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2917 4247 276
Size: 53 83 186 936 1383 1330 2468
Fragments arranged by size:
2468 1383 1330 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI