Restriction map for
pFN21AA0766
(Circular) (Six-base) MAPSORT of: pFN21AA0766.seq Check: 6682 from: 1 to: 6526
pFN21AA0766 6526 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2166 4336 278
Size: 53 83 186 936 630 2170 2468
Fragments arranged by size:
2468 2170 936 630 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2040 2367 3869 1937
Size: 103 327 1502 4594
Fragments arranged by size:
4594 1502 327 103
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 3518 4589 5973 6241 1632
Size: 672 1214 1071 1384 268 1917
Fragments arranged by size:
1917 1384 1214 1071 672 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 5425 6402 1203
Size: 4222 977 1327
Fragments arranged by size:
4222 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 5255 5255
Size: 6526
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3606 1184
Size: 2422 4104
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3425 3457 3425
Size: 32 6494
AlwNI CAG_nnn'CTG
Cuts at: 4269 5841 4269
Size: 1572 4954
ApaI G_GGCC'C
Cuts at: 2116 2116
Size: 6526
ApaLI G'TGCA_C
Cuts at: 4583 5739 4583
Size: 1156 5370
ApoI r'AATT_y
Cuts at: 1493 2954 3262 3413 3835 4061 4213 6322 1493
Size: 1461 308 151 422 226 152 2109 1697
Fragments arranged by size:
2109 1697 1461 422 308 226 152 151
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2309 2405 3851 1870
Size: 73 120 246 96 1446 4545
Fragments arranged by size:
4545 1446 246 120 96 73
BamHI G'GATC_C
Cuts at: 2463 3856 4223 2463
Size: 1393 367 4766
Fragments arranged by size:
4766 1393 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 3847 5302 618
Size: 325 217 627 382 1678 1455 1842
Fragments arranged by size:
1842 1678 1455 627 382 325 217
BanII G_rGCy'C
Cuts at: 1391 2116 2316 2870 3783 3845 1391
Size: 725 200 554 913 62 4072
Fragments arranged by size:
4072 913 725 554 200 62
BbeI G_GCGC'C
Cuts at: 2173 2173
Size: 6526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5555
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4717 4751 1903
Size: 34 2780 34 3678
Fragments arranged by size:
3678 2780 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5137 136
Size: 107 122 71 631 605 3465 1525
Fragments arranged by size:
3465 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2623 6521 2623
Size: 3898 2628
BlpI GC'TnA_GC
Cuts at: 2184 2711 3943 2184
Size: 527 1232 4767
Fragments arranged by size:
4767 1232 527
Bme1580I G_kGCm'C
Cuts at: 1790 2116 4587 5743 1790
Size: 326 2471 1156 2573
Fragments arranged by size:
2573 2471 1156 326
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4257 5215 442
Size: 416 53 83 399 24 2840 958 1753
Fragments arranged by size:
2840 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2313 2313
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2427 5186 1239
Size: 1188 2759 2579
Fragments arranged by size:
2759 2579 1188
Bpu10I CC'TnA_GC
Cuts at: 2468 3029 2468
Size: 561 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4651 5516 5814 4651
Size: 865 298 5363
Fragments arranged by size:
5363 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5189 882
Size: 617 3690 2219
Fragments arranged by size:
3690 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4317 493
Size: 711 3113 2702
Fragments arranged by size:
3113 2702 711
BsaBI GATnn'nnATC
Cuts at: 2886 3381 3738 6520 2886
Size: 495 357 2782 2892
Fragments arranged by size:
2892 2782 495 357
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2163 2170 4333
Size: 53 83 186 936 482 148 7 2163
Cuts at: 4333 4715 5350 275
Size: 382 635 1451
Fragments arranged by size:
2163 1451 936 635 482 382 186 148
83 53 7
BsaWI w'CCGG_w
Cuts at: 2098 4959 5357 5631 5778 6189 2098
Size: 2861 398 274 147 411 2435
Fragments arranged by size:
2861 2435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6301
Fragments arranged by size:
6301 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6526
BseYI C'CCAG_C
Cuts at: 2073 2346 5729 6225 2073
Size: 273 3383 496 2374
Fragments arranged by size:
3383 2374 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2322 1163
Size: 573 586 5367
Fragments arranged by size:
5367 586 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3976 4737 5765 1540
Size: 275 186 1975 761 1028 2301
Fragments arranged by size:
2301 1975 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2870 4587 4672 5743 2190
Size: 680 1717 85 1071 2973
Fragments arranged by size:
2973 1717 1071 680 85
BsmI GAATG_Cn'
Cuts at: 4037 4130 4037
Size: 93 6433
BsmBI CGTCTCn'nnnn_
Cuts at: 2788 3407 2788
Size: 619 5907
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2190 2316 2870 3783 3845 4587
Size: 399 326 74 126 554 913 62 742
Cuts at: 4587 4672 5743 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 913 742 554 399 326 126
85 74 62
BspEI T'CCGG_A
Cuts at: 2098 6189 2098
Size: 4091 2435
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3881 844
Size: 3037 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2203 4413 1394
Size: 429 380 2210 3507
Fragments arranged by size:
3507 2210 429 380
BsrDI GCAATG_nn'
Cuts at: 65 1248 5021 5195 65
Size: 1183 3773 174 1396
Fragments arranged by size:
3773 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5170 5357 1700
Size: 110 3360 187 2869
Fragments arranged by size:
3360 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 2224 1925
Size: 299 6227
BssSI C'ACGA_G
Cuts at: 2298 4586 5598 2298
Size: 2288 1012 3226
Fragments arranged by size:
3226 2288 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6526
BstBI TT'CG_AA
Cuts at: 3249 5330 3249
Size: 2081 4445
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6526
BstYI r'GATC_y
Cuts at: 1972 2463 2623 3856 4223 4608 4625 6077 6163
Size: 491 160 1233 367 385 17 1452 86
Cuts at: 6163 6175 6521 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1233 491 385 367 346 160
86 17 12
BstZ17I GTA'TAC
Cuts at: 2368 2368
Size: 6526
Bsu36I CC'TnA_GG
Cuts at: 3437 3437
Size: 6526
BtgI C'CryG_G
Cuts at: 513 1065 2265 2590 513
Size: 552 1200 325 4449
Fragments arranged by size:
4449 1200 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 4892
Fragments arranged by size:
4892 895 683 56
BtsI GCAGTG_nn'
Cuts at: 4042 4836 4856 4042
Size: 794 20 5712
Fragments arranged by size:
5712 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6526
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6491
DraI TTT'AAA
Cuts at: 2521 3048 3487 3830 2521
Size: 527 439 343 5217
Fragments arranged by size:
5217 527 439 343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5533 809
Size: 507 4217 1802
Fragments arranged by size:
4217 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3973 4862 6491
Size: 54 1425 50 275 18 2143 889 1629
Cuts at: 6491 8
Size: 43
Fragments arranged by size:
2143 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 4456 1353
Size: 927 2176 3423
Fragments arranged by size:
3423 2176 927
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2228 5113 5487 5633 387
Size: 1064 138 639 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 639 374 146 138
EcoICRI GAG'CTC
Cuts at: 2868 2868
Size: 6526
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2304 2427 3518 4589 5186 5973 6241
Size: 393 672 123 1091 1071 597 787 268
Cuts at: 6241 1239
Size: 1524
Fragments arranged by size:
1524 1091 1071 787 672 597 393 268
123
EcoO109I rG'GnC_Cy
Cuts at: 2112 2113 2600 3433 2112
Size: 1 487 833 5205
Fragments arranged by size:
5205 833 487 1
EcoRI G'AATT_C
Cuts at: 3835 4213 3835
Size: 378 6148
EcoRV GAT'ATC
Cuts at: 2449 2449
Size: 6526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2642 2642
Size: 6526
FspI TGC'GCA
Cuts at: 5032 5032
Size: 6526
HaeII r_GCGC'y
Cuts at: 1678 2173 5673 1678
Size: 495 3500 2531
Fragments arranged by size:
3500 2531 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3385 3417 5167 5199 5241
Size: 32 135 32 1813 32 1750 32 42
Cuts at: 5241 5273 1373
Size: 32 2626
Fragments arranged by size:
2626 1813 1750 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 2434 3870 4116 669
Size: 988 281 103 393 1436 246 3079
Fragments arranged by size:
3079 1436 988 393 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KasI G'GCGC_C
Cuts at: 2169 2169
Size: 6526
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 3022 4125 3022
Size: 1103 5423
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2589 2710 2801 5640 5824 1305
Size: 1284 121 91 2839 184 2007
Fragments arranged by size:
2839 2007 1284 184 121 91
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4704
Fragments arranged by size:
4704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2381 2658 4484 4843 5002 518
Size: 648 843 372 277 1826 359 159 2042
Fragments arranged by size:
2042 1826 843 648 372 359 277 159
MspA1I CmG'CkG
Cuts at: 1606 2592 3942 4619 5767 6012 1606
Size: 986 1350 677 1148 245 2120
Fragments arranged by size:
2120 1350 1148 986 677 245
NarI GG'CG_CC
Cuts at: 2170 2170
Size: 6526
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5974
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 2414 3884 5429 1132
Size: 97 1185 1470 1545 2229
Fragments arranged by size:
2229 1545 1470 1185 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6526
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 2820 1108
Size: 1269 443 4814
Fragments arranged by size:
4814 1269 443
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 6108 6140 4595
Size: 32 1481 32 4981
Fragments arranged by size:
4981 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3433 3433
Size: 6526
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PspOMI G'GGCC_C
Cuts at: 2112 2112
Size: 6526
PstI C_TGCA'G
Cuts at: 830 2700 3878 830
Size: 1870 1178 3478
Fragments arranged by size:
3478 1870 1178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
SacI G_AGCT'C
Cuts at: 2870 2870
Size: 6526
SacII CC_GC'GG
Cuts at: 2593 2593
Size: 6526
SalI G'TCGA_C
Cuts at: 1936 2039 3868 1936
Size: 103 1829 4594
Fragments arranged by size:
4594 1829 103
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2696 3874 5009 5690 5881 826
Size: 219 1651 1178 1135 681 191 1471
Fragments arranged by size:
1651 1471 1178 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2171 2171
Size: 6526
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 3853 1872
Size: 1981 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4630 5531 5793 820
Size: 197 926 2687 901 262 1553
Fragments arranged by size:
2687 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3884 1132
Size: 2752 3774
SspI AAT'ATT
Cuts at: 5 52 3057 3366 4450 5
Size: 47 3005 309 1084 2081
Fragments arranged by size:
3005 2081 1084 309 47
StuI AGG'CCT
Cuts at: 1517 2307 1517
Size: 790 5736
StyI C'CwwG_G
Cuts at: 513 1065 2538 3965 513
Size: 552 1473 1427 3074
Fragments arranged by size:
3074 1473 1427 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4553 4570 4723 4908 5353 325
Size: 1255 2973 17 153 185 445 1498
Fragments arranged by size:
2973 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3242 4772 6484
Size: 274 80 33 51 1443 1265 1530 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
1712 1530 1443 1265 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 6486 6518 2749
Size: 32 3705 32 2757
Fragments arranged by size:
3705 2757 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3012 1316
Size: 1696 4830
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3772 3772
Size: 6526
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 3851 1870
Size: 1981 4545
XmnI GAAnn'nnTTC
Cuts at: 2625 3174 4655 2625
Size: 549 1481 4496
Fragments arranged by size:
4496 1481 549
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2164 4334 276
Size: 53 83 186 936 630 2170 2468
Fragments arranged by size:
2468 2170 936 630 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI
BstXI DraIII EcoNI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SrfI SwaI