Restriction map for
pFN21AA0743
(Circular) (Six-base) MAPSORT of: pFN21AA0743.seq Check: 8556 from: 1 to: 7888
pFN21AA0743 7888 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4049 4453 4049
Size: 404 7484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5698 278
Size: 53 83 186 936 4162 2468
Fragments arranged by size:
4162 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2400 4733 5231 1937
Size: 463 2333 498 4594
Fragments arranged by size:
4594 2333 498 463
Acc65I G'GTAC_C
Cuts at: 2511 5209 2511
Size: 2698 5190
AclI AA'CG_TT
Cuts at: 1563 6015 6388 1563
Size: 4452 373 3063
Fragments arranged by size:
4452 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 3530 4234 5951 7335 7603 1632
Size: 1850 48 704 1717 1384 268 1917
Fragments arranged by size:
1917 1850 1717 1384 704 268 48
AflII C'TTAA_G
Cuts at: 820 1017 3248 820
Size: 197 2231 5460
Fragments arranged by size:
5460 2231 197
AflIII A'CryG_T
Cuts at: 1203 2622 6787 7764 1203
Size: 1419 4165 977 1327
Fragments arranged by size:
4165 1419 1327 977
AgeI A'CCGG_T
Cuts at: 6719 6719
Size: 7888
AhdI GACnn_n'nnGTC
Cuts at: 5050 6617 5050
Size: 1567 6321
AleI CACnn'nnGTG
Cuts at: 1166 3806 4259 1166
Size: 2640 453 4795
Fragments arranged by size:
4795 2640 453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2796 1184
Size: 1612 6276
AlwNI CAG_nnn'CTG
Cuts at: 5631 7203 5631
Size: 1572 6316
ApaLI G'TGCA_C
Cuts at: 5945 7101 5945
Size: 1156 6732
ApoI r'AATT_y
Cuts at: 1493 5197 5423 5575 7684 1493
Size: 3704 226 152 2109 1697
Fragments arranged by size:
3704 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6442 160
Size: 872 5410 1606
Fragments arranged by size:
5410 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
AvaI C'yCGr_G
Cuts at: 1870 1943 3765 3918 5213 1870
Size: 73 1822 153 1295 4545
Fragments arranged by size:
4545 1822 1295 153 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3273 3306 3273
Size: 33 7855
BamHI G'GATC_C
Cuts at: 5218 5585 5218
Size: 367 7521
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 2511 2897 3830 5209
Size: 325 217 627 675 49 386 933 1379
Cuts at: 5209 6664 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1379 933 675 627 386 325
217 49
BanII G_rGCy'C
Cuts at: 1391 2015 2294 2325 2459 4593 5138 5207 1391
Size: 624 279 31 134 2134 545 69 4072
Fragments arranged by size:
4072 2134 624 545 279 134 69 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4345 4866 928
Size: 971 2446 521 3950
Fragments arranged by size:
3950 2446 971 521
BbvCI CC'TCA_GC
Cuts at: 3276 3276
Size: 7888
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3203 3237 4699 4733 6079
Size: 34 50 34 1182 34 1462 34 1346
Cuts at: 6079 6113 1903
Size: 34 3678
Fragments arranged by size:
3678 1462 1346 1182 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2897 5780 6990 2897
Size: 2883 1210 3795
Fragments arranged by size:
3795 2883 1210
BclI T'GATC_A
Cuts at: 1548 3705 1548
Size: 2157 5731
BfrBI ATG'CAT
Cuts at: 2992 2992
Size: 7888
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6499 136
Size: 107 122 71 631 605 4827 1525
Fragments arranged by size:
4827 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7883 7883
Size: 7888
BlpI GC'TnA_GC
Cuts at: 5305 5305
Size: 7888
Bme1580I G_kGCm'C
Cuts at: 1790 5949 7105 1790
Size: 4159 1156 2573
Fragments arranged by size:
4159 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7888
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2917 4026 5619
Size: 416 53 83 399 24 1500 1109 1593
Cuts at: 5619 6577 442
Size: 958 1753
Fragments arranged by size:
1753 1593 1500 1109 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 3049 3113 3142 3355 4281 4301 6548
Size: 917 893 64 29 213 926 20 2247
Cuts at: 6548 1239
Size: 2579
Fragments arranged by size:
2579 2247 926 917 893 213 64 29
20
Bpu10I CC'TnA_GC
Cuts at: 2942 3276 3530 2942
Size: 334 254 7300
Fragments arranged by size:
7300 334 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6013 6878 7176 6013
Size: 865 298 6725
Fragments arranged by size:
6725 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 3167 3288 6551 882
Size: 617 735 933 121 3263 2219
Fragments arranged by size:
3263 2219 933 735 617 121
BsaAI yAC'GTr
Cuts at: 493 1204 2707 4158 5185 5679 493
Size: 711 1503 1451 1027 494 2702
Fragments arranged by size:
2702 1503 1451 1027 711 494
BsaBI GATnn'nnATC
Cuts at: 2177 7882 2177
Size: 5705 2183
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3964 4564 5695 6077
Size: 53 83 186 936 2431 600 1131 382
Cuts at: 6077 6712 275
Size: 635 1451
Fragments arranged by size:
2431 1451 1131 936 635 600 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2900 6321 6719 6993 7140 7551 2900
Size: 3421 398 274 147 411 3237
Fragments arranged by size:
3421 3237 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3140 3170 1178
Size: 30 165 30 1737 30 5896
Fragments arranged by size:
5896 1737 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 4403 1186
Size: 1225 1992 4671
Fragments arranged by size:
4671 1992 1225
BseYI C'CCAG_C
Cuts at: 2008 3030 4217 7091 7587 2008
Size: 1022 1187 2874 496 2309
Fragments arranged by size:
2874 2309 1187 1022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2875 1163
Size: 573 1139 6176
Fragments arranged by size:
6176 1139 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5338 6099 7127 1540
Size: 275 186 3337 761 1028 2301
Fragments arranged by size:
3337 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4297 5138 5949 6034 7105 4297
Size: 841 811 85 1071 5080
Fragments arranged by size:
5080 1071 841 811 85
BsmI GAATG_Cn'
Cuts at: 2782 4803 5399 5492 2782
Size: 2021 596 93 5178
Fragments arranged by size:
5178 2021 596 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5063 5063
Size: 7888
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2294 2325 2459 4297 4593 5138
Size: 399 225 279 31 134 1838 296 545
Cuts at: 5138 5207 5949 6034 7105 1391
Size: 69 742 85 1071 2174
Fragments arranged by size:
2174 1838 1071 742 545 399 296 279
225 134 85 69 31
BspEI T'CCGG_A
Cuts at: 7551 7551
Size: 7888
BspHI T'CATG_A
Cuts at: 3336 5115 5777 7507 3336
Size: 1779 662 1730 3717
Fragments arranged by size:
3717 1779 1730 662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2927 4049 4453 5243 844
Size: 2083 1122 404 790 3489
Fragments arranged by size:
3489 2083 1122 790 404
BsrBI CCG'CTC
Cuts at: 1394 1823 4002 5145 5775 1394
Size: 429 2179 1143 630 3507
Fragments arranged by size:
3507 2179 1143 630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3637 3639 4147 6383 6557 65
Size: 1183 2389 2 508 2236 174 1396
Fragments arranged by size:
2389 2236 1396 1183 508 174 2
BsrFI r'CCGG_y
Cuts at: 1700 1810 2894 4503 6532 6719 1700
Size: 110 1084 1609 2029 187 2869
Fragments arranged by size:
2869 2029 1609 1084 187 110
BsrGI T'GTAC_A
Cuts at: 96 2961 3888 5025 96
Size: 2865 927 1137 2959
Fragments arranged by size:
2959 2865 1137 927
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7888
BssSI C'ACGA_G
Cuts at: 3908 5948 6960 3908
Size: 2040 1012 4836
Fragments arranged by size:
4836 2040 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7888
BstBI TT'CG_AA
Cuts at: 6692 6692
Size: 7888
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7888
BstXI CCAn_nnnn'nTGG
Cuts at: 3841 4586 3841
Size: 745 7143
BstYI r'GATC_y
Cuts at: 1972 2601 5218 5585 5970 5987 7439 7525 7537
Size: 629 2617 367 385 17 1452 86 12
Cuts at: 7537 7883 1972
Size: 346 1977
Fragments arranged by size:
2617 1977 1452 629 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2412 2982 3258 4242 4896 513
Size: 552 1347 570 276 984 654 3505
Fragments arranged by size:
3505 1347 984 654 570 552 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3475 487
Size: 683 56 2249 4900
Fragments arranged by size:
4900 2249 683 56
BtsI GCAGTG_nn'
Cuts at: 2828 5404 6198 6218 2828
Size: 2576 794 20 4498
Fragments arranged by size:
4498 2576 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3891 3926 529
Size: 35 3327 35 4491
Fragments arranged by size:
4491 3327 35 35
DraI TTT'AAA
Cuts at: 5192 5192
Size: 7888
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6895 809
Size: 507 5579 1802
Fragments arranged by size:
5579 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 3382 4199
Size: 54 1425 50 275 18 384 1168 817
Cuts at: 4199 4475 5146 5335 6224 7853 8
Size: 276 671 189 889 1629 43
Fragments arranged by size:
1629 1425 1168 889 817 671 384 276
275 189 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5335 1537
Size: 275 3523 4090
Fragments arranged by size:
4090 3523 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2345 3549 3575 3617 3996 4574 5135 5818
Size: 992 1204 26 42 379 578 561 683
Cuts at: 5818 1353
Size: 3423
Fragments arranged by size:
3423 1204 992 683 578 561 379 42
26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 6475 6849 6995 387
Size: 1064 138 3494 1392 374 146 1280
Fragments arranged by size:
3494 1392 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5136 5136
Size: 7888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 3049 3113 3142 3355 3482 3530
Size: 393 524 893 64 29 213 127 48
Cuts at: 3530 4234 4281 4301 5951 6548 7335 7603 1239
Size: 704 47 20 1650 597 787 268 1524
Fragments arranged by size:
1650 1524 893 787 704 597 524 393
268 213 127 64 48 47 29 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2923 2102
Size: 821 7067
EcoO109I rG'GnC_Cy
Cuts at: 4028 4926 5052 4028
Size: 898 126 6864
Fragments arranged by size:
6864 898 126
EcoRI G'AATT_C
Cuts at: 5197 5575 5197
Size: 378 7510
FspI TGC'GCA
Cuts at: 6394 6394
Size: 7888
HaeII r_GCGC'y
Cuts at: 1678 4155 7035 1678
Size: 2477 2880 2531
Fragments arranged by size:
2880 2531 2477
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2525 2557 2996
Size: 32 135 32 869 32 52 32 439
Cuts at: 2996 3028 5036 5068 6529 6561 6603 6635 1373
Size: 32 2008 32 1461 32 42 32 2626
Fragments arranged by size:
2626 2008 1461 869 439 135 52 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3127 3255 4372 4512 5232 5478
Size: 988 281 1189 128 1117 140 720 246
Cuts at: 5478 669
Size: 3079
Fragments arranged by size:
3079 1189 1117 988 720 281 246 140
128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7888
HpaI GTT'AAC
Cuts at: 3127 3255 4512 5478 3127
Size: 128 1257 966 5537
Fragments arranged by size:
5537 1257 966 128
KpnI G_GTAC'C
Cuts at: 2515 5213 2515
Size: 2698 5190
MfeI C'AATT_G
Cuts at: 5487 5487
Size: 7888
MluI A'CGCG_T
Cuts at: 7764 7764
Size: 7888
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4654 7002 7186 1305
Size: 3349 2348 184 2007
Fragments arranged by size:
3349 2348 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 3384 4201 4477 10
Size: 54 1425 343 384 1168 817 276 3421
Fragments arranged by size:
3421 1425 1168 817 384 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2471 2963 2981 2987 3560 3806
Size: 648 1005 300 492 18 6 573 246
Cuts at: 3806 4259 5114 5846 6205 6364 518
Size: 453 855 732 359 159 2042
Fragments arranged by size:
2042 1005 855 732 648 573 492 453
359 300 246 159 18 6
MspA1I CmG'CkG
Cuts at: 1606 2054 2761 5304 5981 7129 7374 1606
Size: 448 707 2543 677 1148 245 2120
Fragments arranged by size:
2543 2120 1148 707 677 448 245
NaeI GCC'GGC
Cuts at: 4505 4505
Size: 7888
NcoI C'CATG_G
Cuts at: 513 1065 2982 513
Size: 552 1917 5419
Fragments arranged by size:
5419 1917 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7888
NgoMIV G'CCGG_C
Cuts at: 4503 4503
Size: 7888
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7888
NotI GC'GGCC_GC
Cuts at: 5335 5335
Size: 7888
NsiI A_TGCA'T
Cuts at: 2994 2994
Size: 7888
NspI r_CATG'y
Cuts at: 1132 1229 2626 2962 3889 4081 5246 6791 1132
Size: 97 1397 336 927 192 1165 1545 2229
Fragments arranged by size:
2229 1545 1397 1165 927 336 192 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7888
PciI A'CATG_T
Cuts at: 2622 6787 2622
Size: 4165 3723
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4304 1108
Size: 3196 4692
PfoI T'CCnGG_A
Cuts at: 2604 4297 4941 2604
Size: 1693 644 5551
Fragments arranged by size:
5551 1693 644
PmeI GTTT'AAAC
Cuts at: 5192 5192
Size: 7888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5957 5989 7470 7502 5957
Size: 32 1481 32 6343
Fragments arranged by size:
6343 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4028 5052 4028
Size: 1024 6864
PshAI GACnn'nnGTC
Cuts at: 1613 2130 1613
Size: 517 7371
PsiI TTA'TAA
Cuts at: 2115 5458 2115
Size: 3343 4545
PstI C_TGCA'G
Cuts at: 830 3393 4464 5240 830
Size: 2563 1071 776 3478
Fragments arranged by size:
3478 2563 1071 776
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7888
PvuII CAG'CTG
Cuts at: 2761 2761
Size: 7888
SacI G_AGCT'C
Cuts at: 5138 5138
Size: 7888
SalI G'TCGA_C
Cuts at: 1936 5230 1936
Size: 3294 4594
SapI GCTCTTCn'nnn_
Cuts at: 3996 5135 3996
Size: 1139 6749
SbfI CC_TGCA'GG
Cuts at: 4464 5240 4464
Size: 776 7112
ScaI AGT'ACT
Cuts at: 4614 6136 7848 4614
Size: 1522 1712 4654
Fragments arranged by size:
4654 1712 1522
SfcI C'TryA_G
Cuts at: 826 1045 3389 4460 4497 4794 5236 6371 7052
Size: 219 2344 1071 37 297 442 1135 681
Cuts at: 7052 7243 826
Size: 191 1471
Fragments arranged by size:
2344 1471 1135 1071 681 442 297 219
191 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7888
SmaI CCC'GGG
Cuts at: 1872 5215 1872
Size: 3343 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3248 3918 5992 6893 7155 820
Size: 197 926 1305 670 2074 901 262 1553
Fragments arranged by size:
2074 1553 1305 926 901 670 262 197
SnaBI TAC'GTA
Cuts at: 493 4158 5679 493
Size: 3665 1521 2702
Fragments arranged by size:
3665 2702 1521
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7888
SphI G_CATG'C
Cuts at: 1132 4081 5246 1132
Size: 2949 1165 3774
Fragments arranged by size:
3774 2949 1165
SspI AAT'ATT
Cuts at: 5 52 4645 5812 5
Size: 47 4593 1167 2081
Fragments arranged by size:
4593 2081 1167 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7888
StyI C'CwwG_G
Cuts at: 513 1065 2982 3880 4573 5327 513
Size: 552 1917 898 693 754 3074
Fragments arranged by size:
3074 1917 898 754 693 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5915 5932 6085 6270 6715 325
Size: 1255 4335 17 153 185 445 1498
Fragments arranged by size:
4335 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2436 2961 3888
Size: 274 80 33 51 1443 459 525 927
Cuts at: 3888 4612 4883 5025 5031 6134 7846 96
Size: 724 271 142 6 1103 1712 138
Fragments arranged by size:
1712 1443 1103 927 724 525 459 274
271 142 138 80 51 33 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4162 4194 7848 7880 4162
Size: 32 3654 32 4170
Fragments arranged by size:
4170 3654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3500 1316
Size: 2184 5704
XbaI T'CTAG_A
Cuts at: 5224 5224
Size: 7888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 4462 4759 3038
Size: 1424 297 6167
Fragments arranged by size:
6167 1424 297
XhoI C'TCGA_G
Cuts at: 1943 3918 1943
Size: 1975 5913
XmaI C'CCGG_G
Cuts at: 1870 5213 1870
Size: 3343 4545
XmnI GAAnn'nnTTC
Cuts at: 2876 6017 2876
Size: 3141 4747
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5696 276
Size: 53 83 186 936 4162 2468
Fragments arranged by size:
4162 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI