Restriction map for
pFN21AA0739
(Circular) (Six-base) MAPSORT of: pFN21AA0739.seq Check: 611 from: 1 to: 7918
pFN21AA0739 7918 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3984 3984
Size: 7918
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5728 278
Size: 53 83 186 936 4192 2468
Fragments arranged by size:
4192 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2943 5190 5261 1937
Size: 1006 2247 71 4594
Fragments arranged by size:
4594 2247 1006 71
Acc65I G'GTAC_C
Cuts at: 5239 5239
Size: 7918
AclI AA'CG_TT
Cuts at: 1563 6045 6418 1563
Size: 4482 373 3063
Fragments arranged by size:
4482 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 3404 3885 5981 7365 7633 1632
Size: 893 879 481 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 893 879 481 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7721
AflIII A'CryG_T
Cuts at: 1203 4642 6817 7794 1203
Size: 3439 2175 977 1327
Fragments arranged by size:
3439 2175 1327 977
AgeI A'CCGG_T
Cuts at: 6749 6749
Size: 7918
AhdI GACnn_n'nnGTC
Cuts at: 6647 6647
Size: 7918
AleI CACnn'nnGTG
Cuts at: 1166 4307 4643 1166
Size: 3141 336 4441
Fragments arranged by size:
4441 3141 336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7918
AlwNI CAG_nnn'CTG
Cuts at: 5661 7233 5661
Size: 1572 6346
ApaLI G'TGCA_C
Cuts at: 4940 5975 7131 4940
Size: 1035 1156 5727
Fragments arranged by size:
5727 1156 1035
ApoI r'AATT_y
Cuts at: 1493 2652 2964 3734 4848 5227 5453 5605 7714
Size: 1159 312 770 1114 379 226 152 2109
Cuts at: 7714 1493
Size: 1697
Fragments arranged by size:
2109 1697 1159 1114 770 379 312 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2607 4401 6472 160
Size: 872 1575 1794 2071 1606
Fragments arranged by size:
2071 1794 1606 1575 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
AvaI C'yCGr_G
Cuts at: 1870 1943 5243 1870
Size: 73 3300 4545
Fragments arranged by size:
4545 3300 73
BamHI G'GATC_C
Cuts at: 3289 5248 5615 3289
Size: 1959 367 5592
Fragments arranged by size:
5592 1959 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2396 5239 6694 618
Size: 325 217 627 609 2843 1455 1842
Fragments arranged by size:
2843 1842 1455 627 609 325 217
BanII G_rGCy'C
Cuts at: 1391 5237 1391
Size: 3846 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4756 928
Size: 971 2857 4090
Fragments arranged by size:
4090 2857 971
BbvCI CC'TCA_GC
Cuts at: 2802 2802
Size: 7918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2249 2283 6109 6143 1903
Size: 34 312 34 3826 34 3678
Fragments arranged by size:
3826 3678 312 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2063 5810 7020 2063
Size: 3747 1210 2961
Fragments arranged by size:
3747 2961 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7918
BfrBI ATG'CAT
Cuts at: 2632 4111 2632
Size: 1479 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6529 136
Size: 107 122 71 631 605 4857 1525
Fragments arranged by size:
4857 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2451 7913 2451
Size: 5462 2456
BlpI GC'TnA_GC
Cuts at: 5087 5335 5087
Size: 248 7670
Bme1580I G_kGCm'C
Cuts at: 1790 4944 5979 7135 1790
Size: 3154 1035 1156 2573
Fragments arranged by size:
3154 2573 1156 1035
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2982 3438 3654
Size: 416 53 83 399 24 1565 456 216
Cuts at: 3654 4084 4353 5649 6607 442
Size: 430 269 1296 958 1753
Fragments arranged by size:
1753 1565 1296 958 456 430 416 399
269 216 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7918
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3066 3245 3521 3066
Size: 179 276 7463
Fragments arranged by size:
7463 276 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2563 3036 3261 3372 3505 4016 4704 4986
Size: 1324 473 225 111 133 511 688 282
Cuts at: 4986 6578 1239
Size: 1592 2579
Fragments arranged by size:
2579 1592 1324 688 511 473 282 225
133 111
Bpu10I CC'TnA_GC
Cuts at: 2591 2802 3977 2591
Size: 211 1175 6532
Fragments arranged by size:
6532 1175 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2084 4195 4514 4640 6043 6908 7206 2084
Size: 2111 319 126 1403 865 298 2796
Fragments arranged by size:
2796 2111 1403 865 319 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2055 3932 6581 882
Size: 617 556 1877 2649 2219
Fragments arranged by size:
2649 2219 1877 617 556
BsaAI yAC'GTr
Cuts at: 493 1204 5709 493
Size: 711 4505 2702
Fragments arranged by size:
4505 2702 711
BsaBI GATnn'nnATC
Cuts at: 7912 7912
Size: 7918
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5725 6107 6742 275
Size: 53 83 186 936 4192 382 635 1451
Fragments arranged by size:
4192 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2298 6351 6749 7023 7170 7581 2298
Size: 4053 398 274 147 411 2635
Fragments arranged by size:
4053 2635 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2845 2875 1178
Size: 30 165 30 1442 30 6221
Fragments arranged by size:
6221 1442 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2034 2853 1186
Size: 848 819 6251
Fragments arranged by size:
6251 848 819
BseYI C'CCAG_C
Cuts at: 4450 4579 7121 7617 4450
Size: 129 2542 496 4751
Fragments arranged by size:
4751 2542 496 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7345
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5368 6129 7157 1540
Size: 275 186 3367 761 1028 2301
Fragments arranged by size:
3367 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 4944 5979 6064 7135 3273
Size: 1671 1035 85 1071 4056
Fragments arranged by size:
4056 1671 1071 1035 85
BsmI GAATG_Cn'
Cuts at: 4103 4676 5429 5522 4103
Size: 573 753 93 6499
Fragments arranged by size:
6499 753 573 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2296 2296
Size: 7918
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3273 4944 5237 5979 6064 7135 1391
Size: 399 1483 1671 293 742 85 1071 2174
Fragments arranged by size:
2174 1671 1483 1071 742 399 293 85
BspEI T'CCGG_A
Cuts at: 7581 7581
Size: 7918
BspHI T'CATG_A
Cuts at: 2425 2955 3174 3193 4767 5807 7537 2425
Size: 530 219 19 1574 1040 1730 2806
Fragments arranged by size:
2806 1730 1574 1040 530 219 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3984 4923 5273 844
Size: 3140 939 350 3489
Fragments arranged by size:
3489 3140 939 350
BsrBI CCG'CTC
Cuts at: 1394 1823 5805 1394
Size: 429 3982 3507
Fragments arranged by size:
3982 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2570 6413 6587 65
Size: 1183 1322 3843 174 1396
Fragments arranged by size:
3843 1396 1322 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2256 6562 6749 1700
Size: 110 446 4306 187 2869
Fragments arranged by size:
4306 2869 446 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7918
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7918
BssSI C'ACGA_G
Cuts at: 5978 6990 5978
Size: 1012 6906
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3827 794
Size: 3033 4885
BstBI TT'CG_AA
Cuts at: 6722 6722
Size: 7918
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7918
BstXI CCAn_nnnn'nTGG
Cuts at: 2995 3152 3285 4579 4702 2995
Size: 157 133 1294 123 6211
Fragments arranged by size:
6211 1294 157 133 123
BstYI r'GATC_y
Cuts at: 1972 2451 3158 3289 4127 5062 5248 5615 6000
Size: 479 707 131 838 935 186 367 385
Cuts at: 6000 6017 7469 7555 7567 7913 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 935 838 707 479 385 367
346 186 131 86 17 12
BstZ17I GTA'TAC
Cuts at: 5191 5191
Size: 7918
BtgI C'CryG_G
Cuts at: 513 1065 3162 4266 513
Size: 552 2097 1104 4165
Fragments arranged by size:
4165 2097 1104 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7179
Fragments arranged by size:
7179 683 56
BtsI GCAGTG_nn'
Cuts at: 5434 6228 6248 5434
Size: 794 20 7104
Fragments arranged by size:
7104 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7883
DraI TTT'AAA
Cuts at: 5222 5222
Size: 7918
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6925 809
Size: 507 5609 1802
Fragments arranged by size:
5609 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2254 2287 3165
Size: 54 1425 50 275 18 424 33 878
Cuts at: 3165 4138 4569 5365 6254 7883 8
Size: 973 431 796 889 1629 43
Fragments arranged by size:
1629 1425 973 889 878 796 431 424
275 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 5365 1537
Size: 275 3553 4090
Fragments arranged by size:
4090 3553 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 2406 2759 4461 4952 5157 5848 1353
Size: 846 207 353 1702 491 205 691 3423
Fragments arranged by size:
3423 1702 846 691 491 353 207 205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6505 6879 7025 387
Size: 1064 138 4916 374 146 1280
Fragments arranged by size:
4916 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 2563 3036 3261 3372 3404 3505
Size: 393 893 38 473 225 111 32 101
Cuts at: 3505 3885 4016 4704 4986 5981 6578 7365 7633
Size: 380 131 688 282 995 597 787 268
Cuts at: 7633 1239
Size: 1524
Fragments arranged by size:
1524 995 893 787 688 597 473 393
380 282 268 225 131 111 101 38
32
EcoNI CCTnn'n_nnAGG
Cuts at: 3040 3257 3040
Size: 217 7701
EcoO109I rG'GnC_Cy
Cuts at: 3472 5199 3472
Size: 1727 6191
EcoRI G'AATT_C
Cuts at: 4848 5227 5605 4848
Size: 379 378 7161
Fragments arranged by size:
7161 379 378
FspI TGC'GCA
Cuts at: 4934 6424 4934
Size: 1490 6428
HaeII r_GCGC'y
Cuts at: 1678 7065 1678
Size: 5387 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2656 2688 3663
Size: 32 135 32 782 32 270 32 975
Cuts at: 3663 3695 4387 4419 6559 6591 6633 6665 1373
Size: 32 692 32 2140 32 42 32 2626
Fragments arranged by size:
2626 2140 975 782 692 270 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4965 5262 5508 669
Size: 988 281 3027 297 246 3079
Fragments arranged by size:
3079 3027 988 297 281 246
HindIII A'AGCT_T
Cuts at: 748 4346 748
Size: 3598 4320
HpaI GTT'AAC
Cuts at: 5508 5508
Size: 7918
KpnI G_GTAC'C
Cuts at: 5243 5243
Size: 7918
MfeI C'AATT_G
Cuts at: 3615 5517 3615
Size: 1902 6016
MluI A'CGCG_T
Cuts at: 7794 7794
Size: 7918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2900 2980 5112 7032 7216 1305
Size: 1595 80 2132 1920 184 2007
Fragments arranged by size:
2132 2007 1920 1595 184 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2289 3167 4571 10
Size: 54 1425 343 457 878 1404 3357
Fragments arranged by size:
3357 1425 1404 878 457 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 3173 3953 4307 4643 5876 6235
Size: 648 1588 419 780 354 336 1233 359
Cuts at: 6235 6394 518
Size: 159 2042
Fragments arranged by size:
2042 1588 1233 780 648 419 359 354
336 159
MspA1I CmG'CkG
Cuts at: 1606 3056 3967 4930 5006 5334 6011 7159 7404
Size: 1450 911 963 76 328 677 1148 245
Cuts at: 7404 1606
Size: 2120
Fragments arranged by size:
2120 1450 1148 963 911 677 328 245
76
NaeI GCC'GGC
Cuts at: 2258 2258
Size: 7918
NcoI C'CATG_G
Cuts at: 513 1065 3162 4266 513
Size: 552 2097 1104 4165
Fragments arranged by size:
4165 2097 1104 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7918
NgoMIV G'CCGG_C
Cuts at: 2256 2256
Size: 7918
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7918
NotI GC'GGCC_GC
Cuts at: 5365 5365
Size: 7918
NsiI A_TGCA'T
Cuts at: 2634 4113 2634
Size: 1479 6439
NspI r_CATG'y
Cuts at: 1132 1229 2420 2636 3556 3958 4646 4925 5276
Size: 97 1191 216 920 402 688 279 351
Cuts at: 5276 6821 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1191 920 688 402 351 279
216 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7918
PciI A'CATG_T
Cuts at: 4642 6817 4642
Size: 2175 5743
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4422 1108
Size: 3314 4604
PfoI T'CCnGG_A
Cuts at: 2622 2667 3277 4681 2622
Size: 45 610 1404 5859
Fragments arranged by size:
5859 1404 610 45
PmeI GTTT'AAAC
Cuts at: 5222 5222
Size: 7918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4512 4544 5987 6019 7500 7532 4512
Size: 32 1443 32 1481 32 4898
Fragments arranged by size:
4898 1481 1443 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7918
PsiI TTA'TAA
Cuts at: 5488 5488
Size: 7918
PstI C_TGCA'G
Cuts at: 830 3995 5270 830
Size: 3165 1275 3478
Fragments arranged by size:
3478 3165 1275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7918
PvuII CAG'CTG
Cuts at: 3056 3967 5006 3056
Size: 911 1039 5968
Fragments arranged by size:
5968 1039 911
SalI G'TCGA_C
Cuts at: 1936 5260 1936
Size: 3324 4594
SapI GCTCTTCn'nnn_
Cuts at: 2199 2406 4461 2199
Size: 207 2055 5656
Fragments arranged by size:
5656 2055 207
SbfI CC_TGCA'GG
Cuts at: 5270 5270
Size: 7918
ScaI AGT'ACT
Cuts at: 3697 4037 4803 6166 7878 3697
Size: 340 766 1363 1712 3737
Fragments arranged by size:
3737 1712 1363 766 340
SexAI A'CCwGG_T
Cuts at: 3258 3258
Size: 7918
SfcI C'TryA_G
Cuts at: 826 1045 3991 4841 5266 6401 7082 7273 826
Size: 219 2946 850 425 1135 681 191 1471
Fragments arranged by size:
2946 1471 1135 850 681 425 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7918
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7918
SmaI CCC'GGG
Cuts at: 1872 5245 1872
Size: 3373 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 4210 4529 4655 6022 6923
Size: 197 926 156 2111 319 126 1367 901
Cuts at: 6923 7185 820
Size: 262 1553
Fragments arranged by size:
2111 1553 1367 926 901 319 262 197
156 126
SnaBI TAC'GTA
Cuts at: 493 5709 493
Size: 5216 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7918
SphI G_CATG'C
Cuts at: 1132 2636 5276 1132
Size: 1504 2640 3774
Fragments arranged by size:
3774 2640 1504
SspI AAT'ATT
Cuts at: 5 52 5842 5
Size: 47 5790 2081
Fragments arranged by size:
5790 2081 47
StuI AGG'CCT
Cuts at: 1517 2343 2984 3072 4731 1517
Size: 826 641 88 1659 4704
Fragments arranged by size:
4704 1659 826 641 88
StyI C'CwwG_G
Cuts at: 513 1065 3162 4266 5037 5154 5357 513
Size: 552 2097 1104 771 117 203 3074
Fragments arranged by size:
3074 2097 1104 771 552 203 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3328 4571 5945 5962 6115 6300 6745
Size: 1255 1748 1243 1374 17 153 185 445
Cuts at: 6745 325
Size: 1498
Fragments arranged by size:
1748 1498 1374 1255 1243 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2174 3540 3695
Size: 274 80 33 51 1443 197 1366 155
Cuts at: 3695 3998 4035 4463 4801 6164 7876 96
Size: 303 37 428 338 1363 1712 138
Fragments arranged by size:
1712 1443 1366 1363 428 338 303 274
197 155 138 80 51 37 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7878 7910 7878
Size: 32 7886
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7918
XbaI T'CTAG_A
Cuts at: 5254 5254
Size: 7918
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4109 4109
Size: 7918
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7918
XmaI C'CCGG_G
Cuts at: 1870 5243 1870
Size: 3373 4545
XmnI GAAnn'nnTTC
Cuts at: 2730 6047 2730
Size: 3317 4601
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5726 276
Size: 53 83 186 936 4192 2468
Fragments arranged by size:
4192 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI