Restriction map for
pFN21AA0727
(Circular) (Six-base) MAPSORT of: pFN21AA0727.seq Check: 6591 from: 1 to: 7723
pFN21AA0727 7723 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5533 278
Size: 53 83 186 936 3997 2468
Fragments arranged by size:
3997 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3732 5066 1937
Size: 1795 1334 4594
Fragments arranged by size:
4594 1795 1334
Acc65I G'GTAC_C
Cuts at: 5044 5044
Size: 7723
AclI AA'CG_TT
Cuts at: 1563 5022 5850 6223 1563
Size: 3459 828 373 3063
Fragments arranged by size:
3459 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 3669 3948 4783 5786 7170 7438 1632
Size: 1640 397 279 835 1003 1384 268 1917
Fragments arranged by size:
1917 1640 1384 1003 835 397 279 268
AflII C'TTAA_G
Cuts at: 820 1017 2420 820
Size: 197 1403 6123
Fragments arranged by size:
6123 1403 197
AflIII A'CryG_T
Cuts at: 1203 6622 7599 1203
Size: 5419 977 1327
Fragments arranged by size:
5419 1327 977
AgeI A'CCGG_T
Cuts at: 6554 6554
Size: 7723
AhdI GACnn_n'nnGTC
Cuts at: 6452 6452
Size: 7723
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7723
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2376 1184
Size: 1192 6531
AlwNI CAG_nnn'CTG
Cuts at: 5466 7038 5466
Size: 1572 6151
ApaLI G'TGCA_C
Cuts at: 4933 5780 6936 4933
Size: 847 1156 5720
Fragments arranged by size:
5720 1156 847
ApoI r'AATT_y
Cuts at: 1493 2027 2741 2839 2846 3489 3516 3712 3935
Size: 534 714 98 7 643 27 196 223
Cuts at: 3935 4038 5032 5258 5410 7519 1493
Size: 103 994 226 152 2109 1697
Fragments arranged by size:
2109 1697 994 714 643 534 226 223
196 152 103 98 27 7
AseI AT'TA_AT
Cuts at: 160 1032 6277 160
Size: 872 5245 1606
Fragments arranged by size:
5245 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2571 4454 4993 5015 5048 1870
Size: 73 129 499 1883 539 22 33 4545
Fragments arranged by size:
4545 1883 539 499 129 73 33 22
BamHI G'GATC_C
Cuts at: 3285 4666 5053 5420 3285
Size: 1381 387 367 5588
Fragments arranged by size:
5588 1381 387 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3825 4136 5044 6499 618
Size: 325 217 627 2038 311 908 1455 1842
Fragments arranged by size:
2038 1842 1455 908 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2710 4461 5042 1391
Size: 1319 1751 581 4072
Fragments arranged by size:
4072 1751 1319 581
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2712 4037 4629 4700 928
Size: 971 813 1325 592 71 3951
Fragments arranged by size:
3951 1325 971 813 592 71
BbvCI CC'TCA_GC
Cuts at: 5006 5006
Size: 7723
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3373 3407 5914 5948 1903
Size: 34 1436 34 2507 34 3678
Fragments arranged by size:
3678 2507 1436 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5615 6825 5615
Size: 1210 6513
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7723
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6334 136
Size: 107 122 71 631 605 4662 1525
Fragments arranged by size:
4662 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7718 7718
Size: 7723
BlpI GC'TnA_GC
Cuts at: 5140 5140
Size: 7723
Bme1580I G_kGCm'C
Cuts at: 1790 4090 4139 4937 5016 5784 6940 1790
Size: 2300 49 798 79 768 1156 2573
Fragments arranged by size:
2573 2300 1156 798 768 79 49
BmgBI CAC'GTC
Cuts at: 1318 4591 1318
Size: 3273 4450
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2373 4898 5454
Size: 416 53 83 399 24 956 2525 556
Cuts at: 5454 6412 442
Size: 958 1753
Fragments arranged by size:
2525 1753 958 956 556 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7723
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2247 2247
Size: 7723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 3515 4445 6383 1239
Size: 1412 864 930 1938 2579
Fragments arranged by size:
2579 1938 1412 930 864
Bpu10I CC'TnA_GC
Cuts at: 5006 5006
Size: 7723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2464 3617 5848 6713 7011 2464
Size: 1153 2231 865 298 3176
Fragments arranged by size:
3176 2231 1153 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2218 6386 882
Size: 617 719 4168 2219
Fragments arranged by size:
4168 2219 719 617
BsaAI yAC'GTr
Cuts at: 493 1204 4282 5514 493
Size: 711 3078 1232 2702
Fragments arranged by size:
3078 2702 1232 711
BsaBI GATnn'nnATC
Cuts at: 7717 7717
Size: 7723
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3027 4251 5530 5912
Size: 53 83 186 936 1494 1224 1279 382
Cuts at: 5912 6547 275
Size: 635 1451
Fragments arranged by size:
1494 1451 1279 1224 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2955 6156 6554 6828 6975 7386 2955
Size: 3201 398 274 147 411 3292
Fragments arranged by size:
3292 3201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2048 2078 2124 2154 4824
Size: 30 165 30 645 30 46 30 2670
Cuts at: 4824 4836 4854 4866 1178
Size: 12 18 12 4035
Fragments arranged by size:
4035 2670 645 165 46 30 30 30
30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2804 4337 1186
Size: 1618 1533 4572
Fragments arranged by size:
4572 1618 1533
BseYI C'CCAG_C
Cuts at: 4362 4968 6926 7422 4362
Size: 606 1958 496 4663
Fragments arranged by size:
4663 1958 606 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2813 4904 4933 1163
Size: 573 1077 2091 29 3953
Fragments arranged by size:
3953 2091 1077 573 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5173 5934 6962 1540
Size: 275 186 3172 761 1028 2301
Fragments arranged by size:
3172 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2710 3633 4937 5784 5869 6940 2710
Size: 923 1304 847 85 1071 3493
Fragments arranged by size:
3493 1304 1071 923 847 85
BsmI GAATG_Cn'
Cuts at: 4377 5234 5327 4377
Size: 857 93 6773
Fragments arranged by size:
6773 857 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2068 3018 4943 4945 2068
Size: 950 1925 2 4846
Fragments arranged by size:
4846 1925 950 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2710 3633 4090 4139 4461 4937 5016
Size: 399 920 923 457 49 322 476 79
Cuts at: 5016 5042 5784 5869 6940 1391
Size: 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 923 920 742 476 457 399
322 85 79 49 26
BspEI T'CCGG_A
Cuts at: 7386 7386
Size: 7723
BspHI T'CATG_A
Cuts at: 5612 7342 5612
Size: 1730 5993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 4401 5078 844
Size: 1631 1926 677 3489
Fragments arranged by size:
3489 1926 1631 677
BsrBI CCG'CTC
Cuts at: 1394 1823 5610 1394
Size: 429 3787 3507
Fragments arranged by size:
3787 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3220 3360 6218 6392 65
Size: 1183 1972 140 2858 174 1396
Fragments arranged by size:
2858 1972 1396 1183 174 140
BsrFI r'CCGG_y
Cuts at: 1700 1810 3822 4197 6367 6554 1700
Size: 110 2012 375 2170 187 2869
Fragments arranged by size:
2869 2170 2012 375 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7723
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7723
BssSI C'ACGA_G
Cuts at: 3335 4226 5783 6795 3335
Size: 891 1557 1012 4263
Fragments arranged by size:
4263 1557 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3881 4415 794
Size: 3087 534 4102
Fragments arranged by size:
4102 3087 534
BstBI TT'CG_AA
Cuts at: 3514 4048 6527 3514
Size: 534 2479 4710
Fragments arranged by size:
4710 2479 534
BstEII G'GTnAC_C
Cuts at: 1184 2525 3673 1184
Size: 1341 1148 5234
Fragments arranged by size:
5234 1341 1148
BstYI r'GATC_y
Cuts at: 1972 2793 3285 4666 5053 5420 5805 5822 7274
Size: 821 492 1381 387 367 385 17 1452
Cuts at: 7274 7360 7372 7718 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1381 821 492 387 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2090 2787 4516 2090
Size: 697 1729 5297
Fragments arranged by size:
5297 1729 697
BtgI C'CryG_G
Cuts at: 513 1065 2004 3121 4246 4422 4944 513
Size: 552 939 1117 1125 176 522 3292
Fragments arranged by size:
3292 1125 1117 939 552 522 176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3549 487
Size: 683 56 2323 4661
Fragments arranged by size:
4661 2323 683 56
BtsI GCAGTG_nn'
Cuts at: 5239 6033 6053 5239
Size: 794 20 6909
Fragments arranged by size:
6909 794 20
ClaI AT'CG_AT
Cuts at: 1554 4606 1554
Size: 3052 4671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4109 4144 529
Size: 35 3545 35 4108
Fragments arranged by size:
4108 3545 35 35
DraI TTT'AAA
Cuts at: 2844 5027 2844
Size: 2183 5540
DraIII CAC_nnn'GTG
Cuts at: 4231 4231
Size: 7723
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4787 6730 809
Size: 507 3471 1943 1802
Fragments arranged by size:
3471 1943 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2364 2964
Size: 54 1425 50 275 18 252 282 600
Cuts at: 2964 4233 4289 5170 6059 7688 8
Size: 1269 56 881 889 1629 43
Fragments arranged by size:
1629 1425 1269 889 881 600 282 275
252 56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5170 1537
Size: 275 3358 4090
Fragments arranged by size:
4090 3358 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 2267 4802 5653 1353
Size: 823 91 2535 851 3423
Fragments arranged by size:
3423 2535 851 823 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 3878 6310 6684 6830 387
Size: 1064 138 435 1854 2432 374 146 1280
Fragments arranged by size:
2432 1854 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2708 2708
Size: 7723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2651 3272 3515 3669 3948 4445 4783
Size: 393 1019 621 243 154 279 497 338
Cuts at: 4783 5786 6383 7170 7438 1239
Size: 1003 597 787 268 1524
Fragments arranged by size:
1524 1019 1003 787 621 597 497 393
338 279 268 243 154
EcoNI CCTnn'n_nnAGG
Cuts at: 4397 4397
Size: 7723
EcoO109I rG'GnC_Cy
Cuts at: 4327 4467 4327
Size: 140 7583
EcoRI G'AATT_C
Cuts at: 2027 2741 3516 3712 3935 5032 5410 2027
Size: 714 775 196 223 1097 378 4340
Fragments arranged by size:
4340 1097 775 714 378 223 196
EcoRV GAT'ATC
Cuts at: 2304 2506 3154 2304
Size: 202 648 6873
Fragments arranged by size:
6873 648 202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2966 2059
Size: 907 6816
FspI TGC'GCA
Cuts at: 6229 6229
Size: 7723
HaeII r_GCGC'y
Cuts at: 1678 3055 3604 4204 4879 6870 1678
Size: 1377 549 600 675 1991 2531
Fragments arranged by size:
2531 1991 1377 675 600 549
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2124 2156 3058
Size: 32 135 32 476 32 44 32 902
Cuts at: 3058 3090 3776 3808 3853 3885 4687 4719 6364
Size: 32 686 32 45 32 802 32 1645
Cuts at: 6364 6396 6438 6470 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1645 902 802 686 476 135 45
44 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3331 5067 5313 669
Size: 988 281 1393 1736 246 3079
Fragments arranged by size:
3079 1736 1393 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2442 2602 748
Size: 1694 160 5869
Fragments arranged by size:
5869 1694 160
HpaI GTT'AAC
Cuts at: 5313 5313
Size: 7723
KpnI G_GTAC'C
Cuts at: 5048 5048
Size: 7723
MfeI C'AATT_G
Cuts at: 2190 5322 2190
Size: 3132 4591
MluI A'CGCG_T
Cuts at: 7599 7599
Size: 7723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2451 3044 4822 6837 7021 1305
Size: 1146 593 1778 2015 184 2007
Fragments arranged by size:
2015 2007 1778 1146 593 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 2966 4235 4291 10
Size: 54 1425 343 252 882 1269 56 3442
Fragments arranged by size:
3442 1425 1269 882 343 252 56 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5681 6040 6199 518
Size: 648 4515 359 159 2042
Fragments arranged by size:
4515 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3244 3277 5139 5816 6964 7209 1606
Size: 1638 33 1862 677 1148 245 2120
Fragments arranged by size:
2120 1862 1638 1148 677 245 33
NaeI GCC'GGC
Cuts at: 3824 3824
Size: 7723
NcoI C'CATG_G
Cuts at: 513 1065 2004 3121 4246 513
Size: 552 939 1117 1125 3990
Fragments arranged by size:
3990 1125 1117 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7723
NgoMIV G'CCGG_C
Cuts at: 3822 3822
Size: 7723
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7723
NotI GC'GGCC_GC
Cuts at: 5170 5170
Size: 7723
NspI r_CATG'y
Cuts at: 1132 1229 4153 5081 6626 1132
Size: 97 2924 928 1545 2229
Fragments arranged by size:
2924 2229 1545 928 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7723
PciI A'CATG_T
Cuts at: 6622 6622
Size: 7723
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4231 1108
Size: 3123 4600
PmeI GTTT'AAAC
Cuts at: 5027 5027
Size: 7723
PmlI CAC'GTG
Cuts at: 4282 4282
Size: 7723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5792 5824 7305 7337 5792
Size: 32 1481 32 6178
Fragments arranged by size:
6178 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7723
PsiI TTA'TAA
Cuts at: 5293 5293
Size: 7723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2634 2666 4051 4083 2634
Size: 32 1385 32 6274
Fragments arranged by size:
6274 1385 32 32
PstI C_TGCA'G
Cuts at: 830 3228 4335 5075 830
Size: 2398 1107 740 3478
Fragments arranged by size:
3478 2398 1107 740
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7723
PvuII CAG'CTG
Cuts at: 3244 3244
Size: 7723
SacI G_AGCT'C
Cuts at: 2710 2710
Size: 7723
SalI G'TCGA_C
Cuts at: 1936 5065 1936
Size: 3129 4594
SbfI CC_TGCA'GG
Cuts at: 5075 5075
Size: 7723
ScaI AGT'ACT
Cuts at: 5971 7683 5971
Size: 1712 6011
SfcI C'TryA_G
Cuts at: 826 1045 3224 4331 5071 6206 6887 7078 826
Size: 219 2179 1107 740 1135 681 191 1471
Fragments arranged by size:
2179 1471 1135 1107 740 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7723
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7723
SmaI CCC'GGG
Cuts at: 1872 5017 5050 1872
Size: 3145 33 4545
Fragments arranged by size:
4545 3145 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2420 2479 2571 3632 5827 6728
Size: 197 926 477 59 92 1061 2195 901
Cuts at: 6728 6990 820
Size: 262 1553
Fragments arranged by size:
2195 1553 1061 926 901 477 262 197
92 59
SnaBI TAC'GTA
Cuts at: 493 5514 493
Size: 5021 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7723
SphI G_CATG'C
Cuts at: 1132 4153 5081 1132
Size: 3021 928 3774
Fragments arranged by size:
3774 3021 928
SspI AAT'ATT
Cuts at: 5 52 5647 5
Size: 47 5595 2081
Fragments arranged by size:
5595 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7723
StyI C'CwwG_G
Cuts at: 513 1065 2004 2629 3036 3121 4071 4167 4246
Size: 552 939 625 407 85 950 96 79
Cuts at: 4246 5162 513
Size: 916 3074
Fragments arranged by size:
3074 950 939 916 625 552 407 96
85 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5750 5767 5920 6105 6550 325
Size: 1255 4170 17 153 185 445 1498
Fragments arranged by size:
4170 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 4680 4906
Size: 274 80 33 51 1443 366 2337 226
Cuts at: 4906 5969 7681 96
Size: 1063 1712 138
Fragments arranged by size:
2337 1712 1443 1063 366 274 226 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4836 4868 7683 7715 4836
Size: 32 2815 32 4844
Fragments arranged by size:
4844 2815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2060 1316
Size: 744 6979
XbaI T'CTAG_A
Cuts at: 5059 5059
Size: 7723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2434
Size: 7723
XhoI C'TCGA_G
Cuts at: 1943 2571 1943
Size: 628 7095
XmaI C'CCGG_G
Cuts at: 1870 5015 5048 1870
Size: 3145 33 4545
Fragments arranged by size:
4545 3145 33
XmnI GAAnn'nnTTC
Cuts at: 3414 4859 5852 3414
Size: 1445 993 5285
Fragments arranged by size:
5285 1445 993
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5531 276
Size: 53 83 186 936 3997 2468
Fragments arranged by size:
3997 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I FseI FspAI KasI NarI
NruI NsiI PacI PfoI PpuMI PspOMI PspXI RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI