Restriction map for
pFN21AA0726
(Circular) (Six-base) MAPSORT of: pFN21AA0726.seq Check: 3459 from: 1 to: 7573
pFN21AA0726 7573 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2180 3052 4031 2180
Size: 872 979 5722
Fragments arranged by size:
5722 979 872
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4916 1937
Size: 2979 4594
Acc65I G'GTAC_C
Cuts at: 4894 4894
Size: 7573
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3841 3896 3906 5636 7020 7288 1632
Size: 2209 55 10 1730 1384 268 1917
Fragments arranged by size:
2209 1917 1730 1384 268 55 10
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 6472 7449 1203
Size: 5269 977 1327
Fragments arranged by size:
5269 1327 977
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 3553 6302 3553
Size: 2749 4824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7573
AlwNI CAG_nnn'CTG
Cuts at: 2780 3727 3934 4462 5316 6888 2780
Size: 947 207 528 854 1572 3465
Fragments arranged by size:
3465 1572 947 854 528 207
ApaI G_GGCC'C
Cuts at: 2357 3102 2357
Size: 745 6828
ApaLI G'TGCA_C
Cuts at: 3005 4109 5630 6786 3005
Size: 1104 1521 1156 3792
Fragments arranged by size:
3792 1521 1156 1104
ApoI r'AATT_y
Cuts at: 1493 4131 4382 4882 5108 5260 7369 1493
Size: 2638 251 500 226 152 2109 1697
Fragments arranged by size:
2638 2109 1697 500 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 3365 4160 4898 1870
Size: 73 1422 795 738 4545
Fragments arranged by size:
4545 1422 795 738 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2435 2468 2435
Size: 33 7540
BamHI G'GATC_C
Cuts at: 4156 4903 5270 4156
Size: 747 367 6459
Fragments arranged by size:
6459 747 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 3047 3064 3345 4894
Size: 325 217 627 440 820 17 281 1549
Cuts at: 4894 6349 618
Size: 1455 1842
Fragments arranged by size:
1842 1549 1455 820 627 440 325 281
217 17
BanII G_rGCy'C
Cuts at: 1391 2357 3095 3102 4218 4892 1391
Size: 966 738 7 1116 674 4072
Fragments arranged by size:
4072 1116 966 738 674 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6602
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 7573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2982 3016 3388 3422 5764 5798 1903
Size: 34 1045 34 372 34 2342 34 3678
Fragments arranged by size:
3678 2342 1045 372 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2816 5465 6675 2816
Size: 2649 1210 3714
Fragments arranged by size:
3714 2649 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7573
BfrBI ATG'CAT
Cuts at: 3647 3647
Size: 7573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6184 136
Size: 107 122 71 631 605 4512 1525
Fragments arranged by size:
4512 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2196 2541 7568 2196
Size: 345 5027 2201
Fragments arranged by size:
5027 2201 345
BlpI GC'TnA_GC
Cuts at: 4445 4990 4445
Size: 545 7028
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2357 3009 3069 3102 3295 4076 4113
Size: 442 125 652 60 33 193 781 37
Cuts at: 4113 5634 6790 1790
Size: 1521 1156 2573
Fragments arranged by size:
2573 1521 1156 781 652 442 193 125
60 37 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2426 2524 2952
Size: 416 53 83 399 24 1009 98 428
Cuts at: 2952 3075 3293 5304 6262 442
Size: 123 218 2011 958 1753
Fragments arranged by size:
2011 1753 1009 958 428 416 399 218
123 98 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4308 4337 4308
Size: 29 7544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2834 3341 3635 3758 4232 4357 4638 4648
Size: 1595 507 294 123 474 125 281 10
Cuts at: 4648 6233 1239
Size: 1585 2579
Fragments arranged by size:
2579 1595 1585 507 474 294 281 125
123 10
Bpu10I CC'TnA_GC
Cuts at: 2700 3604 2700
Size: 904 6669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3305 5698 6563 6861 3305
Size: 2393 865 298 4017
Fragments arranged by size:
4017 2393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2810 3540 3749 4847 6236 882
Size: 617 1311 730 209 1098 1389 2219
Fragments arranged by size:
2219 1389 1311 1098 730 617 209
BsaAI yAC'GTr
Cuts at: 493 1204 3709 3901 4441 5364 493
Size: 711 2505 192 540 923 2702
Fragments arranged by size:
2702 2505 923 711 540 192
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3819 3921 3993 5380
Size: 53 83 186 936 2286 102 72 1387
Cuts at: 5380 5762 6397 275
Size: 382 635 1451
Fragments arranged by size:
2286 1451 1387 936 635 382 186 102
83 72 53
BsaWI w'CCGG_w
Cuts at: 6006 6404 6678 6825 7236 6006
Size: 398 274 147 411 6343
Fragments arranged by size:
6343 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3386 3416 4511 4541 1178
Size: 30 165 30 1983 30 1095 30 4210
Fragments arranged by size:
4210 1983 1095 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3506 1186
Size: 2320 5253
BseYI C'CCAG_C
Cuts at: 2735 2780 3088 3964 4247 4433 4452 4538 4826
Size: 45 308 876 283 186 19 86 288
Cuts at: 4826 6776 7272 2735
Size: 1950 496 3036
Fragments arranged by size:
3036 1950 876 496 308 288 283 186
86 45 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3080 4391 4448 1163
Size: 573 1344 1311 57 4288
Fragments arranged by size:
4288 1344 1311 573 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2488 5023 5784 6812 1540
Size: 275 186 487 2535 761 1028 2301
Fragments arranged by size:
2535 2301 1028 761 487 275 186
BsiHKAI G_wGCw'C
Cuts at: 3009 4113 4218 4446 4587 5634 5719 6790 3009
Size: 1104 105 228 141 1047 85 1071 3792
Fragments arranged by size:
3792 1104 1071 1047 228 141 105 85
BsmI GAATG_Cn'
Cuts at: 5084 5177 5084
Size: 93 7480
BsmBI CGTCTCn'nnnn_
Cuts at: 3880 4639 4834 3880
Size: 759 195 6619
Fragments arranged by size:
6619 759 195
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2357 3009 3069 3095 3102 3295
Size: 399 442 125 652 60 26 7 193
Cuts at: 3295 4076 4113 4218 4446 4587 4892 5634 5719
Size: 781 37 105 228 141 305 742 85
Cuts at: 5719 6790 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 781 742 652 442 399 305
228 193 141 125 105 85 60 37
26 7
BspEI T'CCGG_A
Cuts at: 7236 7236
Size: 7573
BspHI T'CATG_A
Cuts at: 3468 5462 7192 3468
Size: 1994 1730 3849
Fragments arranged by size:
3849 1994 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2180 3052 3316 4031 4928 844
Size: 1336 872 264 715 897 3489
Fragments arranged by size:
3489 1336 897 872 715 264
BsrBI CCG'CTC
Cuts at: 1394 1823 2911 4165 5460 1394
Size: 429 1088 1254 1295 3507
Fragments arranged by size:
3507 1295 1254 1088 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4384 6068 6242 65
Size: 1183 3136 1684 174 1396
Fragments arranged by size:
3136 1684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4643 6217 6404 1700
Size: 110 2833 1574 187 2869
Fragments arranged by size:
2869 2833 1574 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7573
BssSI C'ACGA_G
Cuts at: 5633 6645 5633
Size: 1012 6561
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2696 2777 4462 794
Size: 1902 81 1685 3905
Fragments arranged by size:
3905 1902 1685 81
BstBI TT'CG_AA
Cuts at: 6377 6377
Size: 7573
BstEII G'GTnAC_C
Cuts at: 1184 4472 1184
Size: 3288 4285
BstXI CCAn_nnnn'nTGG
Cuts at: 2222 2875 3088 3431 2222
Size: 653 213 343 6364
Fragments arranged by size:
6364 653 343 213
BstYI r'GATC_y
Cuts at: 1972 2196 2541 2953 3930 4156 4278 4903 5270
Size: 224 345 412 977 226 122 625 367
Cuts at: 5270 5655 5672 7124 7210 7222 7568 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 977 625 412 385 367 346
345 226 224 122 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4283 4283
Size: 7573
BtgI C'CryG_G
Cuts at: 513 1065 2084 2215 3247 3574 4468 4728 513
Size: 552 1019 131 1032 327 894 260 3358
Fragments arranged by size:
3358 1032 1019 894 552 327 260 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6834
Fragments arranged by size:
6834 683 56
BtsI GCAGTG_nn'
Cuts at: 3840 3945 4807 5089 5883 5903 3840
Size: 105 862 282 794 20 5510
Fragments arranged by size:
5510 862 794 282 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7538
DraI TTT'AAA
Cuts at: 4877 4877
Size: 7573
DraIII CAC_nnn'GTG
Cuts at: 2777 4042 2777
Size: 1265 6308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 6580 809
Size: 507 816 4448 1802
Fragments arranged by size:
4448 1802 816 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2877 4363 4815
Size: 54 1425 50 275 18 1047 1486 452
Cuts at: 4815 5020 5909 7538 8
Size: 205 889 1629 43
Fragments arranged by size:
1629 1486 1425 1047 889 452 275 205
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5020 1537
Size: 275 3208 4090
Fragments arranged by size:
4090 3208 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3174 3858 4269 4694 5503 1353
Size: 1821 684 411 425 809 3423
Fragments arranged by size:
3423 1821 809 684 425 411
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2798 6160 6534 6680 387
Size: 1064 138 1209 3362 374 146 1280
Fragments arranged by size:
3362 1280 1209 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4216 4216
Size: 7573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2834 3341 3635 3758 3841 3896 3906
Size: 393 1202 507 294 123 83 55 10
Cuts at: 3906 4232 4357 4638 4648 5636 6233 7020 7288
Size: 326 125 281 10 988 597 787 268
Cuts at: 7288 1239
Size: 1524
Fragments arranged by size:
1524 1202 988 787 597 507 393 326
294 281 268 125 123 83 55 10
10
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 4286 2252
Size: 2034 5539
EcoO109I rG'GnC_Cy
Cuts at: 2353 2354 3099 3477 3825 4659 4678 2353
Size: 1 745 378 348 834 19 5248
Fragments arranged by size:
5248 834 745 378 348 19 1
EcoRI G'AATT_C
Cuts at: 4382 4882 5260 4382
Size: 500 378 6695
Fragments arranged by size:
6695 500 378
FspI TGC'GCA
Cuts at: 4610 6079 4610
Size: 1469 6104
FspAI rTGC'GCAy
Cuts at: 4610 4610
Size: 7573
HaeII r_GCGC'y
Cuts at: 1678 2918 4545 6720 1678
Size: 1240 1627 2175 2531
Fragments arranged by size:
2531 2175 1627 1240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2888 2920 3209 3241 3386
Size: 32 135 32 1316 32 289 32 145
Cuts at: 3386 3418 3539 3571 4175 4207 4833 4865 6214
Size: 32 121 32 604 32 626 32 1349
Cuts at: 6214 6246 6288 6320 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1349 1316 626 604 289 145 135
121 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 4917 5163 669
Size: 988 281 472 2507 246 3079
Fragments arranged by size:
3079 2507 988 472 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KpnI G_GTAC'C
Cuts at: 4898 4898
Size: 7573
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4077 6687 6871 1305
Size: 2772 2610 184 2007
Fragments arranged by size:
2772 2610 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2879 10
Size: 54 1425 343 1047 4704
Fragments arranged by size:
4704 1425 1047 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2114 3429 4091 4265 4559 4562 4718
Size: 648 948 1315 662 174 294 3 156
Cuts at: 4718 5531 5890 6049 518
Size: 813 359 159 2042
Fragments arranged by size:
2042 1315 948 813 662 648 359 294
174 159 156 3
MspA1I CmG'CkG
Cuts at: 1606 2179 2739 3968 4204 4989 5666 6814 7059
Size: 573 560 1229 236 785 677 1148 245
Cuts at: 7059 1606
Size: 2120
Fragments arranged by size:
2120 1229 1148 785 677 573 560 245
236
NaeI GCC'GGC
Cuts at: 4645 4645
Size: 7573
NcoI C'CATG_G
Cuts at: 513 1065 2084 2215 3574 4728 513
Size: 552 1019 131 1359 1154 3358
Fragments arranged by size:
3358 1359 1154 1019 552 131
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7573
NgoMIV G'CCGG_C
Cuts at: 4643 4643
Size: 7573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NsiI A_TGCA'T
Cuts at: 3649 3649
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 2402 3651 3782 4096 4931 6476 1132
Size: 97 1173 1249 131 314 835 1545 2229
Fragments arranged by size:
2229 1545 1249 1173 835 314 131 97
PasI CC'CwG_GG
Cuts at: 1348 3070 1348
Size: 1722 5851
PciI A'CATG_T
Cuts at: 6472 6472
Size: 7573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2780 1108
Size: 1672 5901
PfoI T'CCnGG_A
Cuts at: 4179 4179
Size: 7573
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PmlI CAC'GTG
Cuts at: 3709 4441 3709
Size: 732 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 7155 7187 5642
Size: 32 1481 32 6028
Fragments arranged by size:
6028 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4678 4678
Size: 7573
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7573
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 2353 3098 2353
Size: 745 6828
PspXI vC'TCGA_Gb
Cuts at: 3365 3365
Size: 7573
PstI C_TGCA'G
Cuts at: 830 2625 2865 3777 4200 4925 830
Size: 1795 240 912 423 725 3478
Fragments arranged by size:
3478 1795 912 725 423 240
PvuI CG_AT'CG
Cuts at: 2001 2488 2001
Size: 487 7086
PvuII CAG'CTG
Cuts at: 2739 3968 2739
Size: 1229 6344
SacI G_AGCT'C
Cuts at: 4218 4218
Size: 7573
SalI G'TCGA_C
Cuts at: 1936 4915 1936
Size: 2979 4594
SbfI CC_TGCA'GG
Cuts at: 4925 4925
Size: 7573
ScaI AGT'ACT
Cuts at: 5821 7533 5821
Size: 1712 5861
SexAI A'CCwGG_T
Cuts at: 2773 4038 2773
Size: 1265 6308
SfcI C'TryA_G
Cuts at: 826 1045 2621 2861 3010 3253 3667 3773 4196
Size: 219 1576 240 149 243 414 106 423
Cuts at: 4196 4921 6056 6737 6928 826
Size: 725 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 725 681 423 414 243
240 219 191 149 106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 4900 1872
Size: 3028 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3284 3365 5677 6578 6840 820
Size: 197 926 1341 81 2312 901 262 1553
Fragments arranged by size:
2312 1553 1341 926 901 262 197 81
SnaBI TAC'GTA
Cuts at: 493 5364 493
Size: 4871 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 4931 1132
Size: 3799 3774
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 2336 1517
Size: 819 6754
StyI C'CwwG_G
Cuts at: 513 1065 2084 2160 2215 2283 2880 3541 3574
Size: 552 1019 76 55 68 597 661 33
Cuts at: 3574 4674 4728 5012 513
Size: 1100 54 284 3074
Fragments arranged by size:
3074 1100 1019 661 597 552 284 76
68 55 54 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2581 3062 3065 3459 3554 5600 5617
Size: 1255 1001 481 3 394 95 2046 17
Cuts at: 5617 5770 5955 6400 325
Size: 153 185 445 1498
Fragments arranged by size:
2046 1498 1255 1001 481 445 394 185
153 95 17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2532 2625 3527
Size: 274 80 33 51 1443 555 93 902
Cuts at: 3527 5819 7531 96
Size: 2292 1712 138
Fragments arranged by size:
2292 1712 1443 902 555 274 138 93
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2914 2946 2988 3020 4511 4543 7533 7565 2914
Size: 32 42 32 1491 32 2990 32 2922
Fragments arranged by size:
2990 2922 1491 42 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7573
XbaI T'CTAG_A
Cuts at: 4909 4909
Size: 7573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2947 3572 3886 4689 2290
Size: 657 625 314 803 5174
Fragments arranged by size:
5174 803 657 625 314
XhoI C'TCGA_G
Cuts at: 1943 3365 1943
Size: 1422 6151
XmaI C'CCGG_G
Cuts at: 1870 4898 1870
Size: 3028 4545
XmnI GAAnn'nnTTC
Cuts at: 5702 5702
Size: 7573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BsiWI BstZ17I EcoRV
FalI FseI KasI NarI NruI PacI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI