Restriction map for
pFN21AA0725
(Circular) (Six-base) MAPSORT of: pFN21AA0725.seq Check: 1089 from: 1 to: 6838
pFN21AA0725 6838 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3454 3454
Size: 6838
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 2572 4181 1937
Size: 370 265 1609 4594
Fragments arranged by size:
4594 1609 370 265
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2383 3179 3260 3363 3822 3864 3879 4086
Size: 751 796 81 103 459 42 15 207
Cuts at: 4086 4901 6285 6553 1632
Size: 815 1384 268 1917
Fragments arranged by size:
1917 1384 815 796 751 459 268 207
103 81 42 15
AflII C'TTAA_G
Cuts at: 820 1017 3761 820
Size: 197 2744 3897
Fragments arranged by size:
3897 2744 197
AflIII A'CryG_T
Cuts at: 1203 5737 6714 1203
Size: 4534 977 1327
Fragments arranged by size:
4534 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6838
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6838
AlwNI CAG_nnn'CTG
Cuts at: 2203 2537 3869 4581 6153 2203
Size: 334 1332 712 1572 2888
Fragments arranged by size:
2888 1572 1332 712 334
ApaLI G'TGCA_C
Cuts at: 4895 6051 4895
Size: 1156 5682
ApoI r'AATT_y
Cuts at: 1493 2801 3206 3288 3671 4147 4373 4525 6634
Size: 1308 405 82 383 476 226 152 2109
Cuts at: 6634 1493
Size: 1697
Fragments arranged by size:
2109 1697 1308 476 405 383 226 152
82
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 4163 1870
Size: 73 2220 4545
Fragments arranged by size:
4545 2220 73
BamHI G'GATC_C
Cuts at: 4168 4535 4168
Size: 367 6471
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4159 5614 618
Size: 325 217 627 2372 1455 1842
Fragments arranged by size:
2372 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3817 4157 1391
Size: 2426 340 4072
Fragments arranged by size:
4072 2426 340
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2917 928
Size: 971 1018 4849
Fragments arranged by size:
4849 1018 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5029 5063 1903
Size: 34 3092 34 3678
Fragments arranged by size:
3678 3092 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3049 4730 5940 3049
Size: 1681 1210 3947
Fragments arranged by size:
3947 1681 1210
BclI T'GATC_A
Cuts at: 1548 4123 1548
Size: 2575 4263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5449 136
Size: 107 122 71 631 605 3777 1525
Fragments arranged by size:
3777 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2854 3271 4095 6833 2854
Size: 417 824 2738 2859
Fragments arranged by size:
2859 2738 824 417
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 4899 6055 1790
Size: 3109 1156 2573
Fragments arranged by size:
3109 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6838
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 2320 4066
Size: 416 53 83 399 24 618 285 1746
Cuts at: 4066 4569 5527 442
Size: 503 958 1753
Fragments arranged by size:
1753 1746 958 618 503 416 399 285
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5498 1239
Size: 4259 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4963 5828 6126 4963
Size: 865 298 5675
Fragments arranged by size:
5675 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3352 3905 5501 882
Size: 617 1853 553 1596 2219
Fragments arranged by size:
2219 1853 1596 617 553
BsaAI yAC'GTr
Cuts at: 493 1204 2346 4629 493
Size: 711 1142 2283 2702
Fragments arranged by size:
2702 2283 1142 711
BsaBI GATnn'nnATC
Cuts at: 2853 6832 2853
Size: 3979 2859
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4645 5027 5662 275
Size: 53 83 186 936 3112 382 635 1451
Fragments arranged by size:
3112 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5271 5669 5943 6090 6501 5271
Size: 398 274 147 411 5608
Fragments arranged by size:
5608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2021 2051 3799 3829 1178
Size: 30 165 30 618 30 1748 30 4187
Fragments arranged by size:
4187 1748 618 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2607 1186
Size: 1421 5417
BseYI C'CCAG_C
Cuts at: 2876 6041 6537 2876
Size: 3165 496 3177
Fragments arranged by size:
3177 3165 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 1163
Size: 573 300 5965
Fragments arranged by size:
5965 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3817 4899 4984 6055 3817
Size: 1082 85 1071 4600
Fragments arranged by size:
4600 1082 1071 85
BsmI GAATG_Cn'
Cuts at: 2833 4349 4442 2833
Size: 1516 93 5229
Fragments arranged by size:
5229 1516 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2329 2329
Size: 6838
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3817 4157 4899 4984 6055 1391
Size: 399 2027 340 742 85 1071 2174
Fragments arranged by size:
2174 2027 1071 742 399 340 85
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3454 4193 844
Size: 2610 739 3489
Fragments arranged by size:
3489 2610 739
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3042 5333 5507 65
Size: 1183 1794 2291 174 1396
Fragments arranged by size:
2291 1794 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5482 5669 1700
Size: 110 3672 187 2869
Fragments arranged by size:
3672 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2707 3260 96
Size: 2611 553 3674
Fragments arranged by size:
3674 2611 553
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6838
BstBI TT'CG_AA
Cuts at: 2698 5642 2698
Size: 2944 3894
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstXI CCAn_nnnn'nTGG
Cuts at: 2672 2817 2672
Size: 145 6693
BstYI r'GATC_y
Cuts at: 1972 2047 2854 3271 3521 4095 4168 4535 4920
Size: 75 807 417 250 574 73 367 385
Cuts at: 4920 4937 6389 6475 6487 6833 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 807 574 417 385 367 346
250 86 75 73 17 12
BstZ17I GTA'TAC
Cuts at: 2308 2308
Size: 6838
Bsu36I CC'TnA_GG
Cuts at: 2891 2891
Size: 6838
BtgI C'CryG_G
Cuts at: 513 1065 2559 2665 513
Size: 552 1494 106 4686
Fragments arranged by size:
4686 1494 552 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 2737 4354 5148 5168 2737
Size: 1617 794 20 4407
Fragments arranged by size:
4407 1617 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4285 5174 6803
Size: 54 1425 50 275 18 2455 889 1629
Cuts at: 6803 8
Size: 43
Fragments arranged by size:
2455 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 2582 3903 4768 1353
Size: 849 380 1321 865 3423
Fragments arranged by size:
3423 1321 865 849 380
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3815 3815
Size: 6838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2383 3179 3260 3363 3822 3864 3879
Size: 393 751 796 81 103 459 42 15
Cuts at: 3879 4086 4901 5498 6285 6553 1239
Size: 207 815 597 787 268 1524
Fragments arranged by size:
1524 815 796 787 751 597 459 393
268 207 103 81 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3378 3378
Size: 6838
EcoRI G'AATT_C
Cuts at: 3206 3288 4147 4525 3206
Size: 82 859 378 5519
Fragments arranged by size:
5519 859 378 82
EcoRV GAT'ATC
Cuts at: 3082 3217 3082
Size: 135 6703
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3787 3787
Size: 6838
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2021 2053 3679 3711 5479
Size: 32 135 32 449 32 1626 32 1768
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1768 1626 449 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2573 2614 2815 3901 4182 4428
Size: 988 281 635 41 201 1086 281 246
Cuts at: 4428 669
Size: 3079
Fragments arranged by size:
3079 1086 988 635 281 281 246 201
41
HindIII A'AGCT_T
Cuts at: 748 2504 3834 748
Size: 1756 1330 3752
Fragments arranged by size:
3752 1756 1330
HpaI GTT'AAC
Cuts at: 3901 4428 3901
Size: 527 6311
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3611 5952 6136 1305
Size: 2306 2341 184 2007
Fragments arranged by size:
2341 2306 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5016
Fragments arranged by size:
5016 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 4796 5155 5314 518
Size: 648 2367 1263 359 159 2042
Fragments arranged by size:
2367 2042 1263 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2203 4254 4931 6079 6324 1606
Size: 597 2051 677 1148 245 2120
Fragments arranged by size:
2120 2051 1148 677 597 245
NcoI C'CATG_G
Cuts at: 513 1065 2559 2665 513
Size: 552 1494 106 4686
Fragments arranged by size:
4686 1494 552 106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 2428 4196 5741 1132
Size: 97 1199 1768 1545 2229
Fragments arranged by size:
2229 1768 1545 1199 97
PasI CC'CwG_GG
Cuts at: 1348 4108 1348
Size: 2760 4078
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6838
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2978 3010 2978
Size: 32 6806
PstI C_TGCA'G
Cuts at: 830 2862 3416 4190 830
Size: 2032 554 774 3478
Fragments arranged by size:
3478 2032 774 554
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2203 2203
Size: 6838
SacI G_AGCT'C
Cuts at: 3817 3817
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 2571 4180 1936
Size: 635 1609 4594
Fragments arranged by size:
4594 1609 635
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SfcI C'TryA_G
Cuts at: 826 1045 2750 2858 3008 3412 3577 3637 3992
Size: 219 1705 108 150 404 165 60 355
Cuts at: 3992 4186 5321 6002 6193 826
Size: 194 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 681 404 355 219 194
191 165 150 108 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 4165 1872
Size: 2293 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3761 4942 5843 6105 820
Size: 197 926 1818 1181 901 262 1553
Fragments arranged by size:
1818 1553 1181 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 3142 3610 4762 5
Size: 47 3090 468 1152 2081
Fragments arranged by size:
3090 2081 1152 468 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6838
StyI C'CwwG_G
Cuts at: 513 1065 2559 2665 4277 513
Size: 552 1494 106 1612 3074
Fragments arranged by size:
3074 1612 1494 552 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2572 3282 4865 4882 5035 5220 5665
Size: 1255 992 710 1583 17 153 185 445
Cuts at: 5665 325
Size: 1498
Fragments arranged by size:
1583 1498 1255 992 710 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2707 3260 5084
Size: 274 80 33 51 1443 730 553 1824
Cuts at: 5084 6796 96
Size: 1712 138
Fragments arranged by size:
1824 1712 1443 730 553 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6798 6830 6798
Size: 32 6806
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6838
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3751 3751
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 4163 1870
Size: 2293 4545
XmnI GAAnn'nnTTC
Cuts at: 2805 4967 2805
Size: 2162 4676
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI DraIII EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI