Restriction map for
pFN21AA0720
(Circular) (Six-base) MAPSORT of: pFN21AA0720.seq Check: 7437 from: 1 to: 7891
pFN21AA0720 7891 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4797 4797
Size: 7891
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5701 278
Size: 53 83 186 936 4165 2468
Fragments arranged by size:
4165 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2973 5234 1937
Size: 1036 2261 4594
Fragments arranged by size:
4594 2261 1036
Acc65I G'GTAC_C
Cuts at: 5212 5212
Size: 7891
AclI AA'CG_TT
Cuts at: 1563 6018 6391 1563
Size: 4455 373 3063
Fragments arranged by size:
4455 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2905 3617 3878 4344 5954 7338 7606 1632
Size: 1273 712 261 466 1610 1384 268 1917
Fragments arranged by size:
1917 1610 1384 1273 712 466 268 261
AfeI AGC'GCT
Cuts at: 3414 3731 3414
Size: 317 7574
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7694
AflIII A'CryG_T
Cuts at: 1203 2235 2787 3750 4158 6790 7767 1203
Size: 1032 552 963 408 2632 977 1327
Fragments arranged by size:
2632 1327 1032 977 963 552 408
AgeI A'CCGG_T
Cuts at: 6722 6722
Size: 7891
AhdI GACnn_n'nnGTC
Cuts at: 2665 4105 4480 6620 2665
Size: 1440 375 2140 3936
Fragments arranged by size:
3936 2140 1440 375
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7891
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 7859
AlwNI CAG_nnn'CTG
Cuts at: 2113 3595 3784 3928 4273 4282 4828 4838 5634
Size: 1482 189 144 345 9 546 10 796
Cuts at: 5634 7206 2113
Size: 1572 2798
Fragments arranged by size:
2798 1572 1482 796 546 345 189 144
10 9
ApaI G_GGCC'C
Cuts at: 2130 2709 4702 4947 4969 2130
Size: 579 1993 245 22 5052
Fragments arranged by size:
5052 1993 579 245 22
ApaLI G'TGCA_C
Cuts at: 4242 5948 7104 4242
Size: 1706 1156 5029
Fragments arranged by size:
5029 1706 1156
ApoI r'AATT_y
Cuts at: 1493 2411 3863 5200 5426 5578 7687 1493
Size: 918 1452 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1452 1337 918 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6445 160
Size: 872 5413 1606
Fragments arranged by size:
5413 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7891
AvaI C'yCGr_G
Cuts at: 1870 1943 2819 3039 3425 4490 4695 5056 5216
Size: 73 876 220 386 1065 205 361 160
Cuts at: 5216 1870
Size: 4545
Fragments arranged by size:
4545 1065 876 386 361 220 205 160
73
BamHI G'GATC_C
Cuts at: 5221 5588 5221
Size: 367 7524
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 3899 4631 4841 5212
Size: 325 217 627 1592 520 732 210 371
Cuts at: 5212 6667 618
Size: 1455 1842
Fragments arranged by size:
1842 1592 1455 732 627 520 371 325
217 210
BanII G_rGCy'C
Cuts at: 1391 2021 2130 2306 2709 2832 3141 4254 4702
Size: 630 109 176 403 123 309 1113 448
Cuts at: 4702 4887 4928 4947 4969 5088 5210 1391
Size: 185 41 19 22 119 122 4072
Fragments arranged by size:
4072 1113 630 448 403 309 185 176
123 122 119 109 41 22 19
BbeI G_GCGC'C
Cuts at: 3383 3903 3383
Size: 520 7371
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3093 3680 928
Size: 971 1194 587 5139
Fragments arranged by size:
5139 1194 971 587
BbvCI CC'TCA_GC
Cuts at: 3668 3668
Size: 7891
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6082 6116 1903
Size: 34 4145 34 3678
Fragments arranged by size:
4145 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5783 6993 5783
Size: 1210 6681
BclI T'GATC_A
Cuts at: 1548 3243 1548
Size: 1695 6196
BfrBI ATG'CAT
Cuts at: 2176 2176
Size: 7891
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3043 3698 6502
Size: 107 122 71 631 605 1371 655 2804
Cuts at: 6502 136
Size: 1525
Fragments arranged by size:
2804 1525 1371 655 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7886 7886
Size: 7891
BlpI GC'TnA_GC
Cuts at: 3602 5308 3602
Size: 1706 6185
Bme1580I G_kGCm'C
Cuts at: 1790 2130 2709 4246 4702 4947 4969 5952 7108
Size: 340 579 1537 456 245 22 983 1156
Cuts at: 7108 1790
Size: 2573
Fragments arranged by size:
2573 1537 1156 983 579 456 340 245
22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2480 4513 5622
Size: 416 53 83 399 24 1063 2033 1109
Cuts at: 5622 6580 442
Size: 958 1753
Fragments arranged by size:
2033 1753 1109 1063 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3372 1056
Size: 2316 5575
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 7891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2646 2738 2827 3011 3140 3410 3953 4805
Size: 1407 92 89 184 129 270 543 852
Cuts at: 4805 6551 1239
Size: 1746 2579
Fragments arranged by size:
2579 1746 1407 852 543 270 184 129
92 89
Bpu10I CC'TnA_GC
Cuts at: 3668 5082 3668
Size: 1414 6477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3641 4778 6016 6881 7179 3641
Size: 1137 1238 865 298 4353
Fragments arranged by size:
4353 1238 1137 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4436 4523 5040 5104 6554 882
Size: 617 2937 87 517 64 1450 2219
Fragments arranged by size:
2937 2219 1450 617 517 87 64
BsaAI yAC'GTr
Cuts at: 493 1204 3564 3745 5682 493
Size: 711 2360 181 1937 2702
Fragments arranged by size:
2702 2360 1937 711 181
BsaBI GATnn'nnATC
Cuts at: 7885 7885
Size: 7891
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2730 3380 3810 3900
Size: 53 83 186 936 1197 650 430 90
Cuts at: 3900 5698 6080 6715 275
Size: 1798 382 635 1451
Fragments arranged by size:
1798 1451 1197 936 650 635 430 382
186 90 83 53
BsaWI w'CCGG_w
Cuts at: 2325 4591 6324 6722 6996 7143 7554 2325
Size: 2266 1733 398 274 147 411 2662
Fragments arranged by size:
2662 2266 1733 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3047 3062 3077 3092 3179
Size: 30 165 30 1644 15 15 15 87
Cuts at: 3179 3209 3305 3335 4589 4619 1178
Size: 30 96 30 1254 30 4450
Fragments arranged by size:
4450 1644 1254 165 96 87 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 2303 2306 2309 2312 2383 3086 3089
Size: 1114 3 3 3 3 71 703 3
Cuts at: 3089 3509 3512 3695 3926 4304 4307 4322 4331
Size: 420 3 183 231 378 3 15 9
Cuts at: 4331 4334 4337 4340 4343 4346 4349 4808 1186
Size: 3 3 3 3 3 3 459 4269
Fragments arranged by size:
4269 1114 703 459 420 378 231 183
71 15 9 3 3 3 3 3
3 3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2449 2515 2614 2696 2858 2945 4194 4865 4940
Size: 66 99 82 162 87 1249 671 75
Cuts at: 4940 4946 4950 5106 5130 7094 7590 2449
Size: 6 4 156 24 1964 496 2750
Fragments arranged by size:
2750 1964 1249 671 496 162 156 99
87 82 75 66 24 6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2987 3191 3335 3854 1163
Size: 573 363 888 204 144 519 5200
Fragments arranged by size:
5200 888 573 519 363 204 144
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3792 4603 5341 6102 7130 1540
Size: 275 186 1791 811 738 761 1028 2301
Fragments arranged by size:
2301 1791 1028 811 761 738 275 186
BsiHKAI G_wGCw'C
Cuts at: 2306 2336 3141 4246 4887 5952 6037 7108 2306
Size: 30 805 1105 641 1065 85 1071 3089
Fragments arranged by size:
3089 1105 1071 1065 805 641 85 30
BsmI GAATG_Cn'
Cuts at: 2402 5402 5495 2402
Size: 3000 93 4798
Fragments arranged by size:
4798 3000 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3908 4748 3908
Size: 840 7051
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2130 2306 2336 2709 2832 3141
Size: 399 231 109 176 30 373 123 309
Cuts at: 3141 4246 4254 4702 4887 4928 4947 4969 5088
Size: 1105 8 448 185 41 19 22 119
Cuts at: 5088 5210 5952 6037 7108 1391
Size: 122 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 448 399 373 309
231 185 176 123 122 119 109 85
41 30 22 19 8
BspEI T'CCGG_A
Cuts at: 7554 7554
Size: 7891
BspHI T'CATG_A
Cuts at: 5780 7510 5780
Size: 1730 6161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3285 4797 5026 5058 5246 844
Size: 2441 1512 229 32 188 3489
Fragments arranged by size:
3489 2441 1512 229 188 32
BsrBI CCG'CTC
Cuts at: 1394 1823 3775 4605 5778 1394
Size: 429 1952 830 1173 3507
Fragments arranged by size:
3507 1952 1173 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2508 6386 6560 65
Size: 1183 1260 3878 174 1396
Fragments arranged by size:
3878 1396 1260 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3382 6535 6722 1700
Size: 110 1572 3153 187 2869
Fragments arranged by size:
3153 2869 1572 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7891
BssHII G'CGCG_C
Cuts at: 1925 3688 1925
Size: 1763 6128
BssSI C'ACGA_G
Cuts at: 4067 4713 5951 6963 4067
Size: 646 1238 1012 4995
Fragments arranged by size:
4995 1238 1012 646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2397 3928 4237 4273 794
Size: 1603 1531 309 36 4412
Fragments arranged by size:
4412 1603 1531 309 36
BstBI TT'CG_AA
Cuts at: 6695 6695
Size: 7891
BstEII G'GTnAC_C
Cuts at: 1184 4012 1184
Size: 2828 5063
BstXI CCAn_nnnn'nTGG
Cuts at: 4172 4459 4172
Size: 287 7604
BstYI r'GATC_y
Cuts at: 1972 4000 5221 5588 5973 5990 7442 7528 7540
Size: 2028 1221 367 385 17 1452 86 12
Cuts at: 7540 7886 1972
Size: 346 1977
Fragments arranged by size:
2028 1977 1452 1221 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3113 3944 4932 3113
Size: 831 988 6072
Fragments arranged by size:
6072 988 831
BtgI C'CryG_G
Cuts at: 513 1065 2004 3723 3792 4189 4437 4452 4614
Size: 552 939 1719 69 397 248 15 162
Cuts at: 4614 4846 513
Size: 232 3558
Fragments arranged by size:
3558 1719 939 552 397 248 232 162
69 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7152
Fragments arranged by size:
7152 683 56
BtsI GCAGTG_nn'
Cuts at: 2280 2875 3179 4029 4545 5175 5407 6201 6221
Size: 595 304 850 516 630 232 794 20
Cuts at: 6221 2280
Size: 3950
Fragments arranged by size:
3950 850 794 630 595 516 304 232
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7856
DraI TTT'AAA
Cuts at: 5195 5195
Size: 7891
DraIII CAC_nnn'GTG
Cuts at: 3745 3745
Size: 7891
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2043 6898 809
Size: 507 727 4855 1802
Fragments arranged by size:
4855 1802 727 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2690 2770
Size: 54 1425 50 275 18 249 611 80
Cuts at: 2770 2910 2962 3615 3789 4174 4449 4600 5007
Size: 140 52 653 174 385 275 151 407
Cuts at: 5007 5338 6227 7856 8
Size: 331 889 1629 43
Fragments arranged by size:
1629 1425 889 653 611 407 385 331
275 275 249 174 151 140 80 54
52 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3789 4600 5338 1537
Size: 275 1977 811 738 4090
Fragments arranged by size:
4090 1977 811 738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2324 3023 3470 3551 3998 4281 4308 5030
Size: 971 699 447 81 447 283 27 722
Cuts at: 5030 5821 1353
Size: 791 3423
Fragments arranged by size:
3423 971 791 722 699 447 447 283
81 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3326 3584 4736 6478 6852 6998
Size: 1064 138 1737 258 1152 1742 374 146
Cuts at: 6998 387
Size: 1280
Fragments arranged by size:
1742 1737 1280 1152 1064 374 258 146
138
EcoICRI GAG'CTC
Cuts at: 2304 3139 4885 2304
Size: 835 1746 5310
Fragments arranged by size:
5310 1746 835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2646 2738 2827 2905 3011 3140 3410
Size: 393 1014 92 89 78 106 129 270
Cuts at: 3410 3617 3878 3953 4344 4805 5954 6551 7338
Size: 207 261 75 391 461 1149 597 787
Cuts at: 7338 7606 1239
Size: 268 1524
Fragments arranged by size:
1524 1149 1014 787 597 461 393 391
270 268 261 207 129 106 92 89
78 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2141 2804 3112 3226 4286 5068 2141
Size: 663 308 114 1060 782 4964
Fragments arranged by size:
4964 1060 782 663 308 114
EcoO109I rG'GnC_Cy
Cuts at: 2126 2127 2706 4099 4107 4152 4699 4965 4966
Size: 1 579 1393 8 45 547 266 1
Cuts at: 4966 5061 2126
Size: 95 4956
Fragments arranged by size:
4956 1393 579 547 266 95 45 8
1 1
EcoRI G'AATT_C
Cuts at: 5200 5578 5200
Size: 378 7513
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 7891
FspI TGC'GCA
Cuts at: 6397 6397
Size: 7891
HaeII r_GCGC'y
Cuts at: 1678 2958 3319 3383 3416 3733 3890 3903 7038
Size: 1280 361 64 33 317 157 13 3135
Cuts at: 7038 1678
Size: 2531
Fragments arranged by size:
3135 2531 1280 361 317 157 64 33
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 3047 3079 4091
Size: 32 135 32 1079 32 364 32 1012
Cuts at: 4091 4123 4466 4498 4517 4549 4589 4621 5039
Size: 32 343 32 19 32 40 32 418
Cuts at: 5039 5071 6532 6564 6606 6638 1373
Size: 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 1079 1012 418 364 343 135
42 40 32 32 32 32 32 32
32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 5235 5481 669
Size: 988 281 3297 246 3079
Fragments arranged by size:
3297 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7891
HpaI GTT'AAC
Cuts at: 5481 5481
Size: 7891
KasI G'GCGC_C
Cuts at: 3379 3899 3379
Size: 520 7371
KpnI G_GTAC'C
Cuts at: 5216 5216
Size: 7891
MfeI C'AATT_G
Cuts at: 5490 5490
Size: 7891
MluI A'CGCG_T
Cuts at: 3750 7767 3750
Size: 4017 3874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2571 2772 7005 7189 1305
Size: 1266 201 4233 184 2007
Fragments arranged by size:
4233 2007 1266 201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3617 4176 4451 10
Size: 54 1425 343 1785 559 275 3450
Fragments arranged by size:
3450 1785 1425 559 343 275 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2379 4457 5849 6208 6367 518
Size: 648 1015 198 2078 1392 359 159 2042
Fragments arranged by size:
2078 2042 1392 1015 648 359 198 159
MspA1I CmG'CkG
Cuts at: 1606 2024 2113 2696 2847 2862 2895 2925 2989
Size: 418 89 583 151 15 33 30 64
Cuts at: 2989 3129 3349 3631 3649 3766 3781 3925 4228
Size: 140 220 282 18 117 15 144 303
Cuts at: 4228 4237 4364 4398 4503 4768 4825 4950 4983
Size: 9 127 34 105 265 57 125 33
Cuts at: 4983 5307 5984 7132 7377 1606
Size: 324 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 583 418 324 303 282
265 245 220 151 144 140 127 125
117 105 89 64 57 34 33 33
30 18 15 15 9
NarI GG'CG_CC
Cuts at: 3380 3900 3380
Size: 520 7371
NcoI C'CATG_G
Cuts at: 513 1065 2004 4452 4614 4846 513
Size: 552 939 2448 162 232 3558
Fragments arranged by size:
3558 2448 939 552 232 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7891
NheI G'CTAG_C
Cuts at: 1052 3368 1052
Size: 2316 5575
NotI GC'GGCC_GC
Cuts at: 5338 5338
Size: 7891
NsiI A_TGCA'T
Cuts at: 2178 2178
Size: 7891
NspI r_CATG'y
Cuts at: 1132 1229 2192 2791 3520 3613 3760 5249 6794
Size: 97 963 599 729 93 147 1489 1545
Cuts at: 6794 1132
Size: 2229
Fragments arranged by size:
2229 1545 1489 963 729 599 147 97
93
PasI CC'CwG_GG
Cuts at: 1348 4103 4585 5070 1348
Size: 2755 482 485 4169
Fragments arranged by size:
4169 2755 485 482
PciI A'CATG_T
Cuts at: 2787 6790 2787
Size: 4003 3888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4828 1108
Size: 3720 4171
PfoI T'CCnGG_A
Cuts at: 3333 3333
Size: 7891
PmeI GTTT'AAAC
Cuts at: 5195 5195
Size: 7891
PmlI CAC'GTG
Cuts at: 3564 3745 3564
Size: 181 7710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 5960 5992 7473 7505 3305
Size: 32 2623 32 1481 32 3691
Fragments arranged by size:
3691 2623 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4099 4107 4099
Size: 8 7883
PshAI GACnn'nnGTC
Cuts at: 1613 4046 4503 1613
Size: 2433 457 5001
Fragments arranged by size:
5001 2433 457
PsiI TTA'TAA
Cuts at: 5461 5461
Size: 7891
PspOMI G'GGCC_C
Cuts at: 2126 2705 4698 4943 4965 2126
Size: 579 1993 245 22 5052
Fragments arranged by size:
5052 1993 579 245 22
PstI C_TGCA'G
Cuts at: 830 4278 5023 5243 830
Size: 3448 745 220 3478
Fragments arranged by size:
3478 3448 745 220
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7891
PvuII CAG'CTG
Cuts at: 2113 2696 2847 2895 2989 3129 3349 3925 4228
Size: 583 151 48 94 140 220 576 303
Cuts at: 4228 4237 4364 4768 4825 4950 2113
Size: 9 127 404 57 125 5054
Fragments arranged by size:
5054 583 576 404 303 220 151 140
127 125 94 57 48 9
SacI G_AGCT'C
Cuts at: 2306 3141 4887 2306
Size: 835 1746 5310
Fragments arranged by size:
5310 1746 835
SalI G'TCGA_C
Cuts at: 1936 5233 1936
Size: 3297 4594
SanDI GG'GwC_CC
Cuts at: 4099 4099
Size: 7891
SapI GCTCTTCn'nnn_
Cuts at: 2324 3470 2324
Size: 1146 6745
SbfI CC_TGCA'GG
Cuts at: 5023 5243 5023
Size: 220 7671
ScaI AGT'ACT
Cuts at: 6139 7851 6139
Size: 1712 6179
SfcI C'TryA_G
Cuts at: 826 1045 2578 3010 3419 4146 4274 5019 5239
Size: 219 1533 432 409 727 128 745 220
Cuts at: 5239 6374 7055 7246 826
Size: 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 745 727 681 432 409
220 219 191 128
SfoI GGC'GCC
Cuts at: 3381 3901 3381
Size: 520 7371
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7891
SgrAI Cr'CCGG_yG
Cuts at: 1700 3382 1700
Size: 1682 6209
SmaI CCC'GGG
Cuts at: 1872 2821 3041 3427 5218 1872
Size: 949 220 386 1791 4545
Fragments arranged by size:
4545 1791 949 386 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3656 4793 5995 6896 7158 820
Size: 197 926 1713 1137 1202 901 262 1553
Fragments arranged by size:
1713 1553 1202 1137 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5682 493
Size: 5189 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7891
SphI G_CATG'C
Cuts at: 1132 3760 5249 1132
Size: 2628 1489 3774
Fragments arranged by size:
3774 2628 1489
SspI AAT'ATT
Cuts at: 5 52 5815 5
Size: 47 5763 2081
Fragments arranged by size:
5763 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 2340 2576 3218 4181 1517
Size: 630 193 236 642 963 5227
Fragments arranged by size:
5227 963 642 630 236 193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 3693 4452 4614 4846 5330
Size: 552 939 78 1611 759 162 232 484
Cuts at: 5330 513
Size: 3074
Fragments arranged by size:
3074 1611 939 759 552 484 232 162
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3594 3692 4186 4859 5918 5935 6088
Size: 1255 2014 98 494 673 1059 17 153
Cuts at: 6088 6273 6718 325
Size: 185 445 1498
Fragments arranged by size:
2014 1498 1255 1059 673 494 445 185
153 98 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3513 3981 6137
Size: 274 80 33 51 1443 1536 468 2156
Cuts at: 6137 7849 96
Size: 1712 138
Fragments arranged by size:
2156 1712 1536 1443 468 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3179 3211 5026 5058 7851 7883 3179
Size: 32 1815 32 2793 32 3187
Fragments arranged by size:
3187 2793 1815 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7891
XbaI T'CTAG_A
Cuts at: 5227 5227
Size: 7891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 4444 2272
Size: 2172 5719
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7891
XmaI C'CCGG_G
Cuts at: 1870 2819 3039 3425 5216 1870
Size: 949 220 386 1791 4545
Fragments arranged by size:
4545 1791 949 386 220
XmnI GAAnn'nnTTC
Cuts at: 6020 6020
Size: 7891
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5699 276
Size: 53 83 186 936 4165 2468
Fragments arranged by size:
4165 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PspXI PsrI RsrII SacII
SexAI SfiI SrfI SwaI