Restriction map for
pFN21AA0715
(Circular) (Six-base) MAPSORT of: pFN21AA0715.seq Check: 920 from: 1 to: 9088
pFN21AA0715 9088 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4228 4274 5837 6291 4228
Size: 46 1563 454 7025
Fragments arranged by size:
7025 1563 454 46
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3854 6898 278
Size: 53 83 186 936 2318 3044 2468
Fragments arranged by size:
3044 2468 2318 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4365 5582 6431 1937
Size: 2428 1217 849 4594
Fragments arranged by size:
4594 2428 1217 849
Acc65I G'GTAC_C
Cuts at: 6409 6409
Size: 9088
AclI AA'CG_TT
Cuts at: 1563 7215 7588 1563
Size: 5652 373 3063
Fragments arranged by size:
5652 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3595 4261 6118 7151 8535 8803 1632
Size: 1963 666 1857 1033 1384 268 1917
Fragments arranged by size:
1963 1917 1857 1384 1033 666 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8891
AflIII A'CryG_T
Cuts at: 1203 2653 3102 5364 7987 8964 1203
Size: 1450 449 2262 2623 977 1327
Fragments arranged by size:
2623 2262 1450 1327 977 449
AgeI A'CCGG_T
Cuts at: 7919 7919
Size: 9088
AhdI GACnn_n'nnGTC
Cuts at: 7817 7817
Size: 9088
AleI CACnn'nnGTG
Cuts at: 1166 4217 1166
Size: 3051 6037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2464 4024 1184
Size: 1280 1560 6248
Fragments arranged by size:
6248 1560 1280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2672 2704 2672
Size: 32 9056
AlwNI CAG_nnn'CTG
Cuts at: 2217 3015 3136 3517 4633 4970 4985 6831 8403
Size: 798 121 381 1116 337 15 1846 1572
Cuts at: 8403 2217
Size: 2902
Fragments arranged by size:
2902 1846 1572 1116 798 381 337 121
15
ApaI G_GGCC'C
Cuts at: 3475 3475
Size: 9088
ApaLI G'TGCA_C
Cuts at: 4187 7145 8301 4187
Size: 2958 1156 4974
Fragments arranged by size:
4974 2958 1156
ApoI r'AATT_y
Cuts at: 1493 2463 2750 6397 6623 6775 8884 1493
Size: 970 287 3647 226 152 2109 1697
Fragments arranged by size:
3647 2109 1697 970 287 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4431 7642 160
Size: 872 3399 3211 1606
Fragments arranged by size:
3399 3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9088
AvaI C'yCGr_G
Cuts at: 1870 1943 3179 3537 3944 6413 1870
Size: 73 1236 358 407 2469 4545
Fragments arranged by size:
4545 2469 1236 407 358 73
BamHI G'GATC_C
Cuts at: 2970 3478 4728 6418 6785 2970
Size: 508 1250 1690 367 5273
Fragments arranged by size:
5273 1690 1250 508 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3541 3579 4262 4338 6409
Size: 325 217 627 1754 38 683 76 2071
Cuts at: 6409 7864 618
Size: 1455 1842
Fragments arranged by size:
2071 1842 1754 1455 683 627 325 217
76 38
BanII G_rGCy'C
Cuts at: 1391 3256 3475 3807 4169 4465 5663 6158 6407
Size: 1865 219 332 362 296 1198 495 249
Cuts at: 6407 1391
Size: 4072
Fragments arranged by size:
4072 1865 1198 495 362 332 296 249
219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2332 2801 4413 4680 5012 5753 928
Size: 971 433 469 1612 267 332 741 4263
Fragments arranged by size:
4263 1612 971 741 469 433 332 267
BbvCI CC'TCA_GC
Cuts at: 3902 4581 6256 3902
Size: 679 1675 6734
Fragments arranged by size:
6734 1675 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2872 2906 7279 7313 1903
Size: 34 935 34 4373 34 3678
Fragments arranged by size:
4373 3678 935 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5818 6980 8190 5818
Size: 1162 1210 6716
Fragments arranged by size:
6716 1210 1162
BclI T'GATC_A
Cuts at: 1548 2394 3117 1548
Size: 846 723 7519
Fragments arranged by size:
7519 846 723
BfrBI ATG'CAT
Cuts at: 4001 4518 5609 4001
Size: 517 1091 7480
Fragments arranged by size:
7480 1091 517
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3968 7699 136
Size: 107 122 71 631 605 2296 3731 1525
Fragments arranged by size:
3731 2296 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9083 9083
Size: 9088
BlpI GC'TnA_GC
Cuts at: 3387 3510 6505 3387
Size: 123 2995 5970
Fragments arranged by size:
5970 2995 123
Bme1580I G_kGCm'C
Cuts at: 1790 3088 3475 3538 3546 3582 4191 4265 7149
Size: 1298 387 63 8 36 609 74 2884
Cuts at: 7149 8305 1790
Size: 1156 2573
Fragments arranged by size:
2884 2573 1298 1156 609 387 74 63
36 8
BmgBI CAC'GTC
Cuts at: 1318 2654 1318
Size: 1336 7752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4674 5056
Size: 416 53 83 399 24 1658 1599 382
Cuts at: 5056 6101 6819 7777 442
Size: 1045 718 958 1753
Fragments arranged by size:
1753 1658 1599 1045 958 718 416 399
382 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3433 5792 3433
Size: 2359 6729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2085 2176 3113 3416 3624 3878 4097 4655
Size: 846 91 937 303 208 254 219 558
Cuts at: 4655 4770 5087 5398 7748 1239
Size: 115 317 311 2350 2579
Fragments arranged by size:
2579 2350 937 846 558 317 311 303
254 219 208 115 91
Bpu10I CC'TnA_GC
Cuts at: 3902 3955 4233 4581 5538 6244 6256 3902
Size: 53 278 348 957 706 12 6734
Fragments arranged by size:
6734 957 706 348 278 53 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 3217 3817 5270 6298 7213 8078 8376 2627
Size: 590 600 1453 1028 915 865 298 3339
Fragments arranged by size:
3339 1453 1028 915 865 600 590 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2194 3221 3392 3683 4838 7751 882
Size: 617 695 1027 171 291 1155 2913 2219
Fragments arranged by size:
2913 2219 1155 1027 695 617 291 171
BsaAI yAC'GTr
Cuts at: 493 1204 6879 493
Size: 711 5675 2702
Fragments arranged by size:
5675 2702 711
BsaBI GATnn'nnATC
Cuts at: 2975 3143 9082 2975
Size: 168 5939 2981
Fragments arranged by size:
5939 2981 168
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3851 6895 7277 7912
Size: 53 83 186 936 2318 3044 382 635
Cuts at: 7912 275
Size: 1451
Fragments arranged by size:
3044 2318 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4551 7521 7919 8193 8340 8751 4551
Size: 2970 398 274 147 411 4888
Fragments arranged by size:
4888 2970 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 3281 3311 3517
Size: 30 165 30 1269 30 579 30 206
Cuts at: 3517 3547 3680 3710 4070 4100 5690 5720 1178
Size: 30 133 30 360 30 1590 30 4546
Fragments arranged by size:
4546 1590 1269 579 360 206 165 133
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2561 3535 3538 3727 4118 4274 5469 5768
Size: 1375 974 3 189 391 156 1195 299
Cuts at: 5768 6244 6364 1186
Size: 476 120 3910
Fragments arranged by size:
3910 1375 1195 974 476 391 299 189
156 120 3
BseYI C'CCAG_C
Cuts at: 2557 4120 4411 4724 6286 8291 8787 2557
Size: 1563 291 313 1562 2005 496 2858
Fragments arranged by size:
2858 2005 1563 1562 496 313 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2203 2513 2809 3909 4030 4273 4608
Size: 573 467 310 296 1100 121 243 335
Cuts at: 4608 1163
Size: 5643
Fragments arranged by size:
5643 1100 573 467 335 310 296 243
121
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6538 7299 8327 1540
Size: 275 186 4537 761 1028 2301
Fragments arranged by size:
4537 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3511 4191 5778 6158 6282 6344 7149 7234 8305
Size: 680 1587 380 124 62 805 85 1071
Cuts at: 8305 3511
Size: 4294
Fragments arranged by size:
4294 1587 1071 805 680 380 124 85
62
BsmI GAATG_Cn'
Cuts at: 5020 5609 6599 6692 5020
Size: 589 990 93 7416
Fragments arranged by size:
7416 990 589 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2873 2873
Size: 9088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3088 3256 3475 3511 3538 3546 3582
Size: 399 1298 168 219 36 27 8 36
Cuts at: 3582 3807 4169 4191 4265 4465 5663 5778 6158
Size: 225 362 22 74 200 1198 115 380
Cuts at: 6158 6282 6344 6407 7149 7234 8305 1391
Size: 124 62 63 742 85 1071 2174
Fragments arranged by size:
2174 1298 1198 1071 742 399 380 362
225 219 200 168 124 115 85 74
63 62 36 36 27 22 8
BspEI T'CCGG_A
Cuts at: 4551 8751 4551
Size: 4200 4888
BspHI T'CATG_A
Cuts at: 2391 5148 6977 8707 2391
Size: 2757 1829 1730 2772
Fragments arranged by size:
2772 2757 1829 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 3718 4228 4274 4407 5388 5837 6291
Size: 1381 1493 510 46 133 981 449 454
Cuts at: 6291 6443 844
Size: 152 3489
Fragments arranged by size:
3489 1493 1381 981 510 454 449 152
133 46
BsrBI CCG'CTC
Cuts at: 1394 1823 2935 5091 6975 1394
Size: 429 1112 2156 1884 3507
Fragments arranged by size:
3507 2156 1884 1112 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2551 3208 3741 4518 4776 5531 5742
Size: 1183 1303 657 533 777 258 755 211
Cuts at: 5742 7583 7757 65
Size: 1841 174 1396
Fragments arranged by size:
1841 1396 1303 1183 777 755 657 533
258 211 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3561 4677 6336 7732 7919 1700
Size: 110 1751 1116 1659 1396 187 2869
Fragments arranged by size:
2869 1751 1659 1396 1116 187 110
BsrGI T'GTAC_A
Cuts at: 96 5817 96
Size: 5721 3367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9088
BssSI C'ACGA_G
Cuts at: 5300 7148 8160 5300
Size: 1848 1012 6228
Fragments arranged by size:
6228 1848 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4515 6222 794
Size: 3721 1707 3660
Fragments arranged by size:
3721 3660 1707
BstBI TT'CG_AA
Cuts at: 2461 2691 7892 2461
Size: 230 5201 3657
Fragments arranged by size:
5201 3657 230
BstEII G'GTnAC_C
Cuts at: 1184 3817 1184
Size: 2633 6455
BstXI CCAn_nnnn'nTGG
Cuts at: 3169 4132 5341 3169
Size: 963 1209 6916
Fragments arranged by size:
6916 1209 963
BstYI r'GATC_y
Cuts at: 1972 2970 3282 3478 4728 6418 6785 7170 7187
Size: 998 312 196 1250 1690 367 385 17
Cuts at: 7187 8639 8725 8737 9083 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1690 1452 1250 998 385 367 346
312 196 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 5764 513
Size: 552 939 327 3433 3837
Fragments arranged by size:
3837 3433 939 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3247 6344 487
Size: 683 56 2021 3097 3231
Fragments arranged by size:
3231 3097 2021 683 56
BtsI GCAGTG_nn'
Cuts at: 2048 3921 4018 6604 7398 7418 2048
Size: 1873 97 2586 794 20 3718
Fragments arranged by size:
3718 2586 1873 794 97 20
ClaI AT'CG_AT
Cuts at: 1554 4998 1554
Size: 3444 5644
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5410 5445 529
Size: 35 4846 35 4172
Fragments arranged by size:
4846 4172 35 35
DraI TTT'AAA
Cuts at: 6392 6392
Size: 9088
DraIII CAC_nnn'GTG
Cuts at: 6109 6222 6109
Size: 113 8975
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8095 809
Size: 507 6779 1802
Fragments arranged by size:
6779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 3969 4134
Size: 54 1425 50 275 18 540 1599 165
Cuts at: 4134 5308 5331 5554 6535 7424 9053 8
Size: 1174 23 223 981 889 1629 43
Fragments arranged by size:
1629 1599 1425 1174 981 889 540 275
223 165 54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 6535 1537
Size: 275 4723 4090
Fragments arranged by size:
4723 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2243 3219 3423 3881 4310 4536 4872
Size: 825 65 976 204 458 429 226 336
Cuts at: 4872 5102 5390 5465 5937 6180 7018 1353
Size: 230 288 75 472 243 838 3423
Fragments arranged by size:
3423 976 838 825 472 458 429 336
288 243 230 226 204 75 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7675 8049 8195 387
Size: 1064 138 6086 374 146 1280
Fragments arranged by size:
6086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 6156 6156
Size: 9088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2176 3113 3416 3595 3624 3878
Size: 393 453 91 937 303 179 29 254
Cuts at: 3878 4097 4261 4655 4770 5087 5398 6118 7151
Size: 219 164 394 115 317 311 720 1033
Cuts at: 7151 7748 8535 8803 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1033 937 787 720 597 453 394
393 317 311 303 268 254 219 179
164 115 91 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3128 4256 3128
Size: 1128 7960
EcoO109I rG'GnC_Cy
Cuts at: 3024 3312 4117 4741 6290 3024
Size: 288 805 624 1549 5822
Fragments arranged by size:
5822 1549 805 624 288
EcoRI G'AATT_C
Cuts at: 6397 6775 6397
Size: 378 8710
FspI TGC'GCA
Cuts at: 4508 7594 4508
Size: 3086 6002
HaeII r_GCGC'y
Cuts at: 1678 8235 1678
Size: 6557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2514 2546 2590 2622 3680
Size: 32 135 32 942 32 44 32 1058
Cuts at: 3680 3712 3986 4018 4070 4102 4696 4728 4980
Size: 32 274 32 52 32 594 32 252
Cuts at: 4980 5012 6227 6259 6343 6375 7729 7761 7803
Size: 32 1215 32 84 32 1354 32 42
Cuts at: 7803 7835 1373
Size: 32 2626
Fragments arranged by size:
2626 1354 1215 1058 942 594 274 252
135 84 52 44 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4774 5377 5583 6432 6678 669
Size: 988 281 2836 603 206 849 246 3079
Fragments arranged by size:
3079 2836 988 849 603 281 246 206
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9088
HpaI GTT'AAC
Cuts at: 6678 6678
Size: 9088
KpnI G_GTAC'C
Cuts at: 6413 6413
Size: 9088
MfeI C'AATT_G
Cuts at: 4864 6687 4864
Size: 1823 7265
MluI A'CGCG_T
Cuts at: 8964 8964
Size: 9088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 3364 3666 6029 8202 8386 1305
Size: 1173 886 302 2363 2173 184 2007
Fragments arranged by size:
2363 2173 2007 1173 886 302 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 3971 4136 5310 5333
Size: 54 1425 343 540 1599 165 1174 23
Cuts at: 5333 5556 10
Size: 223 3542
Fragments arranged by size:
3542 1599 1425 1174 540 343 223 165
54 23
MslI CAynn'nnrTG
Cuts at: 518 1166 2534 2843 2981 3353 4006 4217 4433
Size: 648 1368 309 138 372 653 211 216
Cuts at: 4433 7046 7405 7564 518
Size: 2613 359 159 2042
Fragments arranged by size:
2613 2042 1368 653 648 372 359 309
216 211 159 138
MspA1I CmG'CkG
Cuts at: 1606 2092 2140 4769 6504 7181 8329 8574 1606
Size: 486 48 2629 1735 677 1148 245 2120
Fragments arranged by size:
2629 2120 1735 1148 677 486 245 48
NcoI C'CATG_G
Cuts at: 513 1065 2004 2331 513
Size: 552 939 327 7270
Fragments arranged by size:
7270 939 552 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9088
NotI GC'GGCC_GC
Cuts at: 6535 6535
Size: 9088
NsiI A_TGCA'T
Cuts at: 4003 4520 5611 4003
Size: 517 1091 7480
Fragments arranged by size:
7480 1091 517
NspI r_CATG'y
Cuts at: 1132 1229 3106 4057 5239 6182 6446 7991 1132
Size: 97 1877 951 1182 943 264 1545 2229
Fragments arranged by size:
2229 1877 1545 1182 951 943 264 97
PasI CC'CwG_GG
Cuts at: 1348 4258 4294 1348
Size: 2910 36 6142
Fragments arranged by size:
6142 2910 36
PciI A'CATG_T
Cuts at: 3102 7987 3102
Size: 4885 4203
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4349 4985 5737 1108
Size: 3241 636 752 4459
Fragments arranged by size:
4459 3241 752 636
PfoI T'CCnGG_A
Cuts at: 2192 4074 4223 2192
Size: 1882 149 7057
Fragments arranged by size:
7057 1882 149
PmeI GTTT'AAAC
Cuts at: 6392 6392
Size: 9088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2672 2704 7157 7189 8670 8702 2672
Size: 32 4453 32 1481 32 3058
Fragments arranged by size:
4453 3058 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3024 3312 4741 6290 3024
Size: 288 1429 1549 5822
Fragments arranged by size:
5822 1549 1429 288
PshAI GACnn'nnGTC
Cuts at: 1613 4769 1613
Size: 3156 5932
PsiI TTA'TAA
Cuts at: 6658 6658
Size: 9088
PspOMI G'GGCC_C
Cuts at: 3471 3471
Size: 9088
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5354 5386 5354
Size: 32 9056
PstI C_TGCA'G
Cuts at: 830 4686 4793 5518 6440 830
Size: 3856 107 725 922 3478
Fragments arranged by size:
3856 3478 922 725 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9088
RsrII CG'GwC_CG
Cuts at: 5073 5073
Size: 9088
SacI G_AGCT'C
Cuts at: 6158 6158
Size: 9088
SalI G'TCGA_C
Cuts at: 1936 5581 6430 1936
Size: 3645 849 4594
Fragments arranged by size:
4594 3645 849
SanDI GG'GwC_CC
Cuts at: 3312 3312
Size: 9088
SapI GCTCTTCn'nnn_
Cuts at: 3881 4872 5390 5937 3881
Size: 991 518 547 7032
Fragments arranged by size:
7032 991 547 518
SbfI CC_TGCA'GG
Cuts at: 6440 6440
Size: 9088
ScaI AGT'ACT
Cuts at: 6195 7336 9048 6195
Size: 1141 1712 6235
Fragments arranged by size:
6235 1712 1141
SfcI C'TryA_G
Cuts at: 826 1045 3000 4042 4204 4682 4789 4901 5514
Size: 219 1955 1042 162 478 107 112 613
Cuts at: 5514 6436 7571 8252 8443 826
Size: 922 1135 681 191 1471
Fragments arranged by size:
1955 1471 1135 1042 922 681 613 478
219 191 162 112 107
SfiI GGCCn_nnn'nGGCC
Cuts at: 3968 3968
Size: 9088
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9088
SmaI CCC'GGG
Cuts at: 1872 3539 6415 1872
Size: 1667 2876 4545
Fragments arranged by size:
4545 2876 1667
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 3196 3832 5285 6313 7192
Size: 197 926 699 554 636 1453 1028 879
Cuts at: 7192 8093 8355 820
Size: 901 262 1553
Fragments arranged by size:
1553 1453 1028 926 901 879 699 636
554 262 197
SnaBI TAC'GTA
Cuts at: 493 6879 493
Size: 6386 2702
SpeI A'CTAG_T
Cuts at: 152 4216 152
Size: 4064 5024
SphI G_CATG'C
Cuts at: 1132 5239 6446 1132
Size: 4107 1207 3774
Fragments arranged by size:
4107 3774 1207
SspI AAT'ATT
Cuts at: 5 52 7012 5
Size: 47 6960 2081
Fragments arranged by size:
6960 2081 47
StuI AGG'CCT
Cuts at: 1517 4141 6067 1517
Size: 2624 1926 4538
Fragments arranged by size:
4538 2624 1926
StyI C'CwwG_G
Cuts at: 513 1065 2004 2331 3088 3660 3714 4098 4371
Size: 552 939 327 757 572 54 384 273
Cuts at: 4371 6527 513
Size: 2156 3074
Fragments arranged by size:
3074 2156 939 757 572 552 384 327
273 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3245 4356 4375 4823 5061 5854 6120
Size: 1255 1665 1111 19 448 238 793 266
Cuts at: 6120 7115 7132 7285 7470 7915 325
Size: 995 17 153 185 445 1498
Fragments arranged by size:
1665 1498 1255 1111 995 793 448 445
266 238 185 153 19 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3288 5346 5796
Size: 274 80 33 51 1443 1311 2058 450
Cuts at: 5796 5817 6193 7334 9046 96
Size: 21 376 1141 1712 138
Fragments arranged by size:
2058 1712 1443 1311 1141 450 376 274
138 80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 3517 3549 9048 9080 2012
Size: 32 1473 32 5499 32 2020
Fragments arranged by size:
5499 2020 1473 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3694 4307 4385 1316
Size: 2378 613 78 6019
Fragments arranged by size:
6019 2378 613 78
XbaI T'CTAG_A
Cuts at: 4480 6424 4480
Size: 1944 7144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2786 2365
Size: 421 8667
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9088
XmaI C'CCGG_G
Cuts at: 1870 3537 6413 1870
Size: 1667 2876 4545
Fragments arranged by size:
4545 2876 1667
XmnI GAAnn'nnTTC
Cuts at: 2666 4692 7217 2666
Size: 2026 2525 4537
Fragments arranged by size:
4537 2525 2026
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3852 6896 276
Size: 53 83 186 936 2318 3044 2468
Fragments arranged by size:
3044 2468 2318 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PvuII SacII SexAI SfoI
SrfI SwaI