Restriction map for
pFN21AA0714
(Circular) (Six-base) MAPSORT of: pFN21AA0714.seq Check: 129 from: 1 to: 10003
pFN21AA0714 10003 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7813 278
Size: 53 83 186 936 6277 2468
Fragments arranged by size:
6277 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5443 7119 7346 1937
Size: 3506 1676 227 4594
Fragments arranged by size:
4594 3506 1676 227
Acc65I G'GTAC_C
Cuts at: 7324 7324
Size: 10003
AclI AA'CG_TT
Cuts at: 1563 7126 8130 8503 1563
Size: 5563 1004 373 3063
Fragments arranged by size:
5563 3063 1004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2565 3195 3554 3660 3723 4275 4554
Size: 555 378 630 359 106 63 552 279
Cuts at: 4554 4733 4989 5385 6163 8066 9450 9718 1632
Size: 179 256 396 778 1903 1384 268 1917
Fragments arranged by size:
1917 1903 1384 778 630 555 552 396
378 359 279 268 256 179 106 63
AflII C'TTAA_G
Cuts at: 820 1017 4151 820
Size: 197 3134 6672
Fragments arranged by size:
6672 3134 197
AflIII A'CryG_T
Cuts at: 1203 4345 5197 8902 9879 1203
Size: 3142 852 3705 977 1327
Fragments arranged by size:
3705 3142 1327 977 852
AgeI A'CCGG_T
Cuts at: 8834 8834
Size: 10003
AhdI GACnn_n'nnGTC
Cuts at: 8732 8732
Size: 10003
AleI CACnn'nnGTG
Cuts at: 1166 5107 1166
Size: 3941 6062
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5838 1184
Size: 4654 5349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7005 7037 7005
Size: 32 9971
AlwNI CAG_nnn'CTG
Cuts at: 2102 2297 3524 4000 4442 7746 9318 2102
Size: 195 1227 476 442 3304 1572 2787
Fragments arranged by size:
3304 2787 1572 1227 476 442 195
ApaLI G'TGCA_C
Cuts at: 8060 9216 8060
Size: 1156 8847
ApoI r'AATT_y
Cuts at: 1493 2261 2328 2340 2663 2753 3154 4141 4642
Size: 768 67 12 323 90 401 987 501
Cuts at: 4642 5426 5930 7223 7312 7538 7690 9799 1493
Size: 784 504 1293 89 226 152 2109 1697
Fragments arranged by size:
2109 1697 1293 987 784 768 504 501
401 323 226 152 90 89 67 12
AseI AT'TA_AT
Cuts at: 160 1032 3810 6452 8557 160
Size: 872 2778 2642 2105 1606
Fragments arranged by size:
2778 2642 2105 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10003
AvaI C'yCGr_G
Cuts at: 1870 1943 6888 6900 7328 1870
Size: 73 4945 12 428 4545
Fragments arranged by size:
4945 4545 428 73 12
AvrII C'CTAG_G
Cuts at: 2137 2137
Size: 10003
BamHI G'GATC_C
Cuts at: 7333 7700 7333
Size: 367 9636
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7324 8779 618
Size: 325 217 627 5537 1455 1842
Fragments arranged by size:
5537 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2900 3587 6642 7322 1391
Size: 1509 687 3055 680 4072
Fragments arranged by size:
4072 3055 1509 687 680
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3783 6417 928
Size: 971 1884 2634 4514
Fragments arranged by size:
4514 2634 1884 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8194 8228 1903
Size: 34 6257 34 3678
Fragments arranged by size:
6257 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5878 6425 7895 9105 5878
Size: 547 1470 1210 6776
Fragments arranged by size:
6776 1470 1210 547
BclI T'GATC_A
Cuts at: 1548 2350 3310 6250 1548
Size: 802 960 2940 5301
Fragments arranged by size:
5301 2940 960 802
BfrBI ATG'CAT
Cuts at: 2510 2510
Size: 10003
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8614 136
Size: 107 122 71 631 605 6942 1525
Fragments arranged by size:
6942 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3492 5473 9998 3492
Size: 1981 4525 3497
Fragments arranged by size:
4525 3497 1981
BlpI GC'TnA_GC
Cuts at: 2821 4501 7096 7420 2821
Size: 1680 2595 324 5404
Fragments arranged by size:
5404 2595 1680 324
Bme1580I G_kGCm'C
Cuts at: 1790 8064 9220 1790
Size: 6274 1156 2573
Fragments arranged by size:
6274 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3165 1318
Size: 1847 8156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5666 6986 7734
Size: 416 53 83 399 24 4249 1320 748
Cuts at: 7734 8692 442
Size: 958 1753
Fragments arranged by size:
4249 1753 1320 958 748 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10003
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 4899 2857
Size: 2042 7961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2765 6125 6626 8663 1239
Size: 1526 3360 501 2037 2579
Fragments arranged by size:
3360 2579 2037 1526 501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5093 8128 8993 9291 5093
Size: 3035 865 298 5805
Fragments arranged by size:
5805 3035 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5274 8666 882
Size: 617 3775 3392 2219
Fragments arranged by size:
3775 3392 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5184 5198 7794 493
Size: 711 3980 14 2596 2702
Fragments arranged by size:
3980 2702 2596 711 14
BsaBI GATnn'nnATC
Cuts at: 4815 9997 4815
Size: 5182 4821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7810 8192 8827 275
Size: 53 83 186 936 6277 382 635 1451
Fragments arranged by size:
6277 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 5679 8436 8834 9108 9255 9666 4071
Size: 1608 2757 398 274 147 411 4408
Fragments arranged by size:
4408 2757 1608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 7005 7035 1178
Size: 30 165 30 5602 30 4146
Fragments arranged by size:
5602 4146 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2762 3744 6164 6650 1186
Size: 1576 982 2420 486 4539
Fragments arranged by size:
4539 2420 1576 982 486
BseYI C'CCAG_C
Cuts at: 2944 3176 3699 4988 9206 9702 2944
Size: 232 523 1289 4218 496 3245
Fragments arranged by size:
4218 3245 1289 523 496 232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4001 1163
Size: 573 2265 7165
Fragments arranged by size:
7165 2265 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7453 8214 9242 1540
Size: 275 186 5452 761 1028 2301
Fragments arranged by size:
5452 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3587 4069 4570 5437 5753 6642 8064 8149 9220
Size: 482 501 867 316 889 1422 85 1071
Cuts at: 9220 3587
Size: 4370
Fragments arranged by size:
4370 1422 1071 889 867 501 482 316
85
BsmI GAATG_Cn'
Cuts at: 2859 3251 6028 6333 7514 7607 2859
Size: 392 2777 305 1181 93 5255
Fragments arranged by size:
5255 2777 1181 392 305 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3171 4365 7286 3171
Size: 1194 2921 5888
Fragments arranged by size:
5888 2921 1194
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2900 3587 4069 4570 5437 5753 6642
Size: 399 1110 687 482 501 867 316 889
Cuts at: 6642 7322 8064 8149 9220 1391
Size: 680 742 85 1071 2174
Fragments arranged by size:
2174 1110 1071 889 867 742 687 680
501 482 399 316 85
BspEI T'CCGG_A
Cuts at: 9666 9666
Size: 10003
BspHI T'CATG_A
Cuts at: 2353 4405 7892 9622 2353
Size: 2052 3487 1730 2734
Fragments arranged by size:
3487 2734 2052 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5455 7358 844
Size: 4611 1903 3489
Fragments arranged by size:
4611 3489 1903
BsrBI CCG'CTC
Cuts at: 1394 1823 7890 1394
Size: 429 6067 3507
Fragments arranged by size:
6067 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6033 8498 8672 65
Size: 1183 4785 2465 174 1396
Fragments arranged by size:
4785 2465 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8647 8834 1700
Size: 110 6837 187 2869
Fragments arranged by size:
6837 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2276 3961 4059 6844 7242 96
Size: 2180 1685 98 2785 398 2857
Fragments arranged by size:
2857 2785 2180 1685 398 98
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10003
BssSI C'ACGA_G
Cuts at: 8063 9075 8063
Size: 1012 8991
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2507 794
Size: 1713 8290
BstBI TT'CG_AA
Cuts at: 8807 8807
Size: 10003
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10003
BstXI CCAn_nnnn'nTGG
Cuts at: 2952 5881 7076 2952
Size: 2929 1195 5879
Fragments arranged by size:
5879 2929 1195
BstYI r'GATC_y
Cuts at: 1972 3492 5473 7333 7700 8085 8102 9554 9640
Size: 1520 1981 1860 367 385 17 1452 86
Cuts at: 9640 9652 9998 1972
Size: 12 346 1977
Fragments arranged by size:
1981 1977 1860 1520 1452 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 5444 5444
Size: 10003
Bsu36I CC'TnA_GG
Cuts at: 2042 2042
Size: 10003
BtgI C'CryG_G
Cuts at: 513 1065 2004 6735 513
Size: 552 939 4731 3781
Fragments arranged by size:
4731 3781 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9264
Fragments arranged by size:
9264 683 56
BtsI GCAGTG_nn'
Cuts at: 4456 4901 5102 5114 6766 6820 7034 7519 8313
Size: 445 201 12 1652 54 214 485 794
Cuts at: 8313 8333 4456
Size: 20 6126
Fragments arranged by size:
6126 1652 794 485 445 214 201 54
20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10003
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9968
DraI TTT'AAA
Cuts at: 3895 7307 3895
Size: 3412 6591
DraIII CAC_nnn'GTG
Cuts at: 2624 5265 6407 2624
Size: 2641 1142 6220
Fragments arranged by size:
6220 2641 1142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2363 2936 9010 809
Size: 507 1047 573 6074 1802
Fragments arranged by size:
6074 1802 1047 573 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2059 2321 7450
Size: 54 1425 50 275 18 229 262 5129
Cuts at: 7450 8339 9968 8
Size: 889 1629 43
Fragments arranged by size:
5129 1629 1425 889 275 262 229 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7450 1537
Size: 275 5638 4090
Fragments arranged by size:
5638 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3214 3279 3575 4152 4326 4890 6182 6282
Size: 1861 65 296 577 174 564 1292 100
Cuts at: 6282 6330 7933 1353
Size: 48 1603 3423
Fragments arranged by size:
3423 1861 1603 1292 577 564 296 174
100 65 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7048 8590 8964 9110 387
Size: 1064 138 5459 1542 374 146 1280
Fragments arranged by size:
5459 1542 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3585 6640 3585
Size: 3055 6948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2565 2765 3195 3554 3660 3723
Size: 393 555 378 200 430 359 106 63
Cuts at: 3723 4275 4554 4733 4989 5385 6125 6163 6626
Size: 552 279 179 256 396 740 38 463
Cuts at: 6626 8066 8663 9450 9718 1239
Size: 1440 597 787 268 1524
Fragments arranged by size:
1524 1440 787 740 597 555 552 463
430 396 393 378 359 279 268 256
200 179 106 63 38
EcoNI CCTnn'n_nnAGG
Cuts at: 3560 3560
Size: 10003
EcoO109I rG'GnC_Cy
Cuts at: 6601 6613 6601
Size: 12 9991
EcoRI G'AATT_C
Cuts at: 4141 7312 7690 4141
Size: 3171 378 6454
Fragments arranged by size:
6454 3171 378
EcoRV GAT'ATC
Cuts at: 4222 4744 4222
Size: 522 9481
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 4147 2327
Size: 1820 8183
FspI TGC'GCA
Cuts at: 8509 8509
Size: 10003
HaeII r_GCGC'y
Cuts at: 1678 9150 1678
Size: 7472 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 3518 3550 4262
Size: 32 135 32 1298 32 616 32 712
Cuts at: 4262 4294 6423 6455 8644 8676 8718 8750 1373
Size: 32 2129 32 2189 32 42 32 2626
Fragments arranged by size:
2626 2189 2129 1298 712 616 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3186 4243 7347 7593 669
Size: 988 281 1248 1057 3104 246 3079
Fragments arranged by size:
3104 3079 1248 1057 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2385 3956 4327 748
Size: 1637 1571 371 6424
Fragments arranged by size:
6424 1637 1571 371
HpaI GTT'AAC
Cuts at: 7593 7593
Size: 10003
KpnI G_GTAC'C
Cuts at: 7328 7328
Size: 10003
MfeI C'AATT_G
Cuts at: 2934 7602 2934
Size: 4668 5335
MluI A'CGCG_T
Cuts at: 9879 9879
Size: 10003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 5748 6358 6599 9117 9301 1305
Size: 1815 2628 610 241 2518 184 2007
Fragments arranged by size:
2628 2518 2007 1815 610 241 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2323 10
Size: 54 1425 343 491 7690
Fragments arranged by size:
7690 1425 491 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3006 5107 7074 7961 8320 8479 518
Size: 648 1840 2101 1967 887 359 159 2042
Fragments arranged by size:
2101 2042 1967 1840 887 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2489 5230 5835 6646 7419 8096 9244 9489
Size: 883 2741 605 811 773 677 1148 245
Cuts at: 9489 1606
Size: 2120
Fragments arranged by size:
2741 2120 1148 883 811 773 677 605
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 6735 513
Size: 552 939 4731 3781
Fragments arranged by size:
4731 3781 939 552
NdeI CA'TA_TG
Cuts at: 387 6213 6711 387
Size: 5826 498 3679
Fragments arranged by size:
5826 3679 498
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10003
NotI GC'GGCC_GC
Cuts at: 7450 7450
Size: 10003
NsiI A_TGCA'T
Cuts at: 2512 2512
Size: 10003
NspI r_CATG'y
Cuts at: 1132 1229 4173 4193 4349 7215 7361 8906 1132
Size: 97 2944 20 156 2866 146 1545 2229
Fragments arranged by size:
2944 2866 2229 1545 156 146 97 20
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10003
PciI A'CATG_T
Cuts at: 4345 8902 4345
Size: 4557 5446
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10003
PfoI T'CCnGG_A
Cuts at: 5588 5588
Size: 10003
PmeI GTTT'AAAC
Cuts at: 7307 7307
Size: 10003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3285 3317 3708 3740 7005 7037 8072 8104 9585
Size: 32 391 32 3265 32 1035 32 1481
Cuts at: 9585 9617 3285
Size: 32 3671
Fragments arranged by size:
3671 3265 1481 1035 391 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 6601 6613 6601
Size: 12 9991
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10003
PsiI TTA'TAA
Cuts at: 2600 3260 5334 6950 7573 2600
Size: 660 2074 1616 623 5030
Fragments arranged by size:
5030 2074 1616 660 623
PspXI vC'TCGA_Gb
Cuts at: 6888 6888
Size: 10003
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2260 2292 6936 6968 2260
Size: 32 4644 32 5295
Fragments arranged by size:
5295 4644 32 32
PstI C_TGCA'G
Cuts at: 830 2071 2589 5109 7050 7355 830
Size: 1241 518 2520 1941 305 3478
Fragments arranged by size:
3478 2520 1941 1241 518 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10003
PvuII CAG'CTG
Cuts at: 2489 5230 5835 6646 2489
Size: 2741 605 811 5846
Fragments arranged by size:
5846 2741 811 605
SacI G_AGCT'C
Cuts at: 3587 6642 3587
Size: 3055 6948
SalI G'TCGA_C
Cuts at: 1936 7345 1936
Size: 5409 4594
SapI GCTCTTCn'nnn_
Cuts at: 3214 3575 6282 3214
Size: 361 2707 6935
Fragments arranged by size:
6935 2707 361
SbfI CC_TGCA'GG
Cuts at: 7355 7355
Size: 10003
ScaI AGT'ACT
Cuts at: 8251 9963 8251
Size: 1712 8291
SfcI C'TryA_G
Cuts at: 826 1045 2067 2585 5105 7046 7204 7351 8486
Size: 219 1022 518 2520 1941 158 147 1135
Cuts at: 8486 9167 9358 826
Size: 681 191 1471
Fragments arranged by size:
2520 1941 1471 1135 1022 681 518 219
191 158 147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10003
SmaI CCC'GGG
Cuts at: 1872 7330 1872
Size: 5458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4151 5072 6888 6900 8107 9008
Size: 197 926 2208 921 1816 12 1207 901
Cuts at: 9008 9270 820
Size: 262 1553
Fragments arranged by size:
2208 1816 1553 1207 926 921 901 262
197 12
SnaBI TAC'GTA
Cuts at: 493 5184 7794 493
Size: 4691 2610 2702
Fragments arranged by size:
4691 2702 2610
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10003
SphI G_CATG'C
Cuts at: 1132 7361 1132
Size: 6229 3774
SspI AAT'ATT
Cuts at: 5 52 5179 5571 6781 7927 5
Size: 47 5127 392 1210 1146 2081
Fragments arranged by size:
5127 2081 1210 1146 392 47
StuI AGG'CCT
Cuts at: 1517 2047 3744 6190 1517
Size: 530 1697 2446 5330
Fragments arranged by size:
5330 2446 1697 530
StyI C'CwwG_G
Cuts at: 513 1065 2004 2137 4122 4205 4225 5941 6735
Size: 552 939 133 1985 83 20 1716 794
Cuts at: 6735 7442 513
Size: 707 3074
Fragments arranged by size:
3074 1985 1716 939 794 707 552 133
83 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 5648 7082 8030 8047 8200 8385
Size: 1255 1953 2115 1434 948 17 153 185
Cuts at: 8385 8830 325
Size: 445 1498
Fragments arranged by size:
2115 1953 1498 1434 1255 948 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2276 2962 3961
Size: 274 80 33 51 1443 299 686 999
Cuts at: 3961 4059 5000 5200 6844 6854 7242 8249 9961
Size: 98 941 200 1644 10 388 1007 1712
Cuts at: 9961 96
Size: 138
Fragments arranged by size:
1712 1644 1443 1007 999 941 686 388
299 274 200 138 98 80 51 33
10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6745 6777 9963 9995 6745
Size: 32 3186 32 6753
Fragments arranged by size:
6753 3186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3782 1316
Size: 2466 7537
XbaI T'CTAG_A
Cuts at: 4798 7339 4798
Size: 2541 7462
XhoI C'TCGA_G
Cuts at: 1943 6888 6900 1943
Size: 4945 12 5046
Fragments arranged by size:
5046 4945 12
XmaI C'CCGG_G
Cuts at: 1870 7328 1870
Size: 5458 4545
XmnI GAAnn'nnTTC
Cuts at: 5163 5305 7223 8132 5163
Size: 142 1918 909 7034
Fragments arranged by size:
7034 1918 909 142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7811 276
Size: 53 83 186 936 6277 2468
Fragments arranged by size:
6277 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI