Restriction map for
pFN21AA0713
(Circular) (Six-base) MAPSORT of: pFN21AA0713.seq Check: 9909 from: 1 to: 7576
pFN21AA0713 7576 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5386 278
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3141 4919 1937
Size: 1204 1778 4594
Fragments arranged by size:
4594 1778 1204
Acc65I G'GTAC_C
Cuts at: 4897 4897
Size: 7576
AclI AA'CG_TT
Cuts at: 1563 4510 5703 6076 1563
Size: 2947 1193 373 3063
Fragments arranged by size:
3063 2947 1193 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3496 4658 5639 7023 7291 1632
Size: 1864 1162 981 1384 268 1917
Fragments arranged by size:
1917 1864 1384 1162 981 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7379
AflIII A'CryG_T
Cuts at: 1203 3555 6475 7452 1203
Size: 2352 2920 977 1327
Fragments arranged by size:
2920 2352 1327 977
AgeI A'CCGG_T
Cuts at: 6407 6407
Size: 7576
AhdI GACnn_n'nnGTC
Cuts at: 6305 6305
Size: 7576
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7576
AlwNI CAG_nnn'CTG
Cuts at: 3329 3961 4265 4553 5319 6891 3329
Size: 632 304 288 766 1572 4014
Fragments arranged by size:
4014 1572 766 632 304 288
ApaLI G'TGCA_C
Cuts at: 2644 5633 6789 2644
Size: 2989 1156 3431
Fragments arranged by size:
3431 2989 1156
ApoI r'AATT_y
Cuts at: 1493 2775 3336 3634 4775 4885 5111 5263 7372
Size: 1282 561 298 1141 110 226 152 2109
Cuts at: 7372 1493
Size: 1697
Fragments arranged by size:
2109 1697 1282 1141 561 298 226 152
110
AseI AT'TA_AT
Cuts at: 160 1032 2789 2820 4443 6130 160
Size: 872 1757 31 1623 1687 1606
Fragments arranged by size:
1757 1687 1623 1606 872 31
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
AvaI C'yCGr_G
Cuts at: 1870 1943 4901 1870
Size: 73 2958 4545
Fragments arranged by size:
4545 2958 73
BamHI G'GATC_C
Cuts at: 3581 4906 5273 3581
Size: 1325 367 5884
Fragments arranged by size:
5884 1325 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2291 4231 4897 6352 618
Size: 325 217 627 504 1940 666 1455 1842
Fragments arranged by size:
1940 1842 1455 666 627 504 325 217
BanII G_rGCy'C
Cuts at: 1391 4895 1391
Size: 3504 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4726 928
Size: 971 2827 3778
Fragments arranged by size:
3778 2827 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5767 5801 1903
Size: 34 3830 34 3678
Fragments arranged by size:
3830 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3221 5468 6678 3221
Size: 2247 1210 4119
Fragments arranged by size:
4119 2247 1210
BclI T'GATC_A
Cuts at: 1548 2554 3721 1548
Size: 1006 1167 5403
Fragments arranged by size:
5403 1167 1006
BfrBI ATG'CAT
Cuts at: 3740 3947 4523 3740
Size: 207 576 6793
Fragments arranged by size:
6793 576 207
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6187 136
Size: 107 122 71 631 605 4515 1525
Fragments arranged by size:
4515 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2578 2957 7571 2578
Size: 379 4614 2583
Fragments arranged by size:
4614 2583 379
BlpI GC'TnA_GC
Cuts at: 3546 4993 3546
Size: 1447 6129
Bme1580I G_kGCm'C
Cuts at: 1790 2648 5637 6793 1790
Size: 858 2989 1156 2573
Fragments arranged by size:
2989 2573 1156 858
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5307 6265 442
Size: 416 53 83 399 24 3890 958 1753
Fragments arranged by size:
3890 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2739 3207 6236 1239
Size: 1061 439 468 3029 2579
Fragments arranged by size:
3029 2579 1061 468 439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2359 3025 3847 4043 4836 5701 6566 6864 2359
Size: 666 822 196 793 865 865 298 3071
Fragments arranged by size:
3071 865 865 822 793 666 298 196
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3621 6239 882
Size: 617 2122 2618 2219
Fragments arranged by size:
2618 2219 2122 617
BsaAI yAC'GTr
Cuts at: 493 1204 5367 493
Size: 711 4163 2702
Fragments arranged by size:
4163 2702 711
BsaBI GATnn'nnATC
Cuts at: 7570 7570
Size: 7576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5383 5765 6400 275
Size: 53 83 186 936 3850 382 635 1451
Fragments arranged by size:
3850 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6009 6407 6681 6828 7239 6009
Size: 398 274 147 411 6346
Fragments arranged by size:
6346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7351
Fragments arranged by size:
7351 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3797 1186
Size: 2611 4965
BseYI C'CCAG_C
Cuts at: 3991 6779 7275 3991
Size: 2788 496 4292
Fragments arranged by size:
4292 2788 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7003
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5026 5787 6815 1540
Size: 275 186 3025 761 1028 2301
Fragments arranged by size:
3025 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2648 5637 5722 6793 2648
Size: 2989 85 1071 3431
Fragments arranged by size:
3431 2989 1071 85
BsmI GAATG_Cn'
Cuts at: 3132 3526 4801 5087 5180 3132
Size: 394 1275 286 93 5528
Fragments arranged by size:
5528 1275 394 286 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2648 4895 5637 5722 6793 1391
Size: 399 858 2247 742 85 1071 2174
Fragments arranged by size:
2247 2174 1071 858 742 399 85
BspEI T'CCGG_A
Cuts at: 7239 7239
Size: 7576
BspHI T'CATG_A
Cuts at: 3424 5465 7195 3424
Size: 2041 1730 3805
Fragments arranged by size:
3805 2041 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4931 844
Size: 4087 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5463 1394
Size: 429 3640 3507
Fragments arranged by size:
3640 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6071 6245 65
Size: 1183 4823 174 1396
Fragments arranged by size:
4823 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3762 6220 6407 1700
Size: 110 1952 2458 187 2869
Fragments arranged by size:
2869 2458 1952 187 110
BsrGI T'GTAC_A
Cuts at: 96 3757 96
Size: 3661 3915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7576
BssSI C'ACGA_G
Cuts at: 2769 5636 6648 2769
Size: 2867 1012 3697
Fragments arranged by size:
3697 2867 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3746 794
Size: 2952 4624
BstBI TT'CG_AA
Cuts at: 4579 6380 4579
Size: 1801 5775
BstEII G'GTnAC_C
Cuts at: 1184 3235 1184
Size: 2051 5525
BstYI r'GATC_y
Cuts at: 1972 2578 2957 3430 3565 3581 3937 4906 5273
Size: 606 379 473 135 16 356 969 367
Cuts at: 5273 5658 5675 7127 7213 7225 7571 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 969 606 473 385 379 367
356 346 135 86 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 2675 2675
Size: 7576
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7024
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2391 4256 487
Size: 683 56 1165 1865 3807
Fragments arranged by size:
3807 1865 1165 683 56
BtsI GCAGTG_nn'
Cuts at: 5092 5886 5906 5092
Size: 794 20 6762
Fragments arranged by size:
6762 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7541
DraI TTT'AAA
Cuts at: 4880 4880
Size: 7576
DraIII CAC_nnn'GTG
Cuts at: 4009 4009
Size: 7576
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4165 6583 809
Size: 507 2849 2418 1802
Fragments arranged by size:
2849 2418 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4529 5023 5912
Size: 54 1425 50 275 18 2699 494 889
Cuts at: 5912 7541 8
Size: 1629 43
Fragments arranged by size:
2699 1629 1425 889 494 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5023 1537
Size: 275 3211 4090
Fragments arranged by size:
4090 3211 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2418 5506 1353
Size: 1065 3088 3423
Fragments arranged by size:
3423 3088 1065
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6163 6537 6683 387
Size: 1064 138 4574 374 146 1280
Fragments arranged by size:
4574 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2739 3207 3496 4658 5639 6236
Size: 393 668 439 468 289 1162 981 597
Cuts at: 6236 7023 7291 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1162 981 787 668 597 468 439
393 289 268
EcoRI G'AATT_C
Cuts at: 2775 4885 5263 2775
Size: 2110 378 5088
Fragments arranged by size:
5088 2110 378
EcoRV GAT'ATC
Cuts at: 2983 2983
Size: 7576
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4694 4694
Size: 7576
FspI TGC'GCA
Cuts at: 3536 6082 3536
Size: 2546 5030
FspAI rTGC'GCAy
Cuts at: 3536 3536
Size: 7576
HaeII r_GCGC'y
Cuts at: 1678 4859 6723 1678
Size: 3181 1864 2531
Fragments arranged by size:
3181 2531 1864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2736 2768 3587
Size: 32 135 32 797 32 335 32 819
Cuts at: 3587 3619 6217 6249 6291 6323 1373
Size: 32 2598 32 42 32 2626
Fragments arranged by size:
2626 2598 819 797 335 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 4459 4468 4920 5166 669
Size: 988 281 72 2449 9 452 246 3079
Fragments arranged by size:
3079 2449 988 452 281 246 72 9
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7576
HpaI GTT'AAC
Cuts at: 2010 5166 2010
Size: 3156 4420
KpnI G_GTAC'C
Cuts at: 4901 4901
Size: 7576
MfeI C'AATT_G
Cuts at: 5175 5175
Size: 7576
MluI A'CGCG_T
Cuts at: 7452 7452
Size: 7576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2234 3313 3458 6690 6874 1305
Size: 929 1079 145 3232 184 2007
Fragments arranged by size:
3232 2007 1079 929 184 145
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4531 10
Size: 54 1425 343 2699 3055
Fragments arranged by size:
3055 2699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 5534 5893 6052 518
Size: 648 1671 2697 359 159 2042
Fragments arranged by size:
2697 2042 1671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2972 3326 4992 5669 6817 7062 1606
Size: 1366 354 1666 677 1148 245 2120
Fragments arranged by size:
2120 1666 1366 1148 677 354 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7024
NdeI CA'TA_TG
Cuts at: 387 2030 2834 3736 4429 387
Size: 1643 804 902 693 3534
Fragments arranged by size:
3534 1643 902 804 693
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7576
NotI GC'GGCC_GC
Cuts at: 5023 5023
Size: 7576
NsiI A_TGCA'T
Cuts at: 3742 3949 4525 3742
Size: 207 576 6793
Fragments arranged by size:
6793 576 207
NspI r_CATG'y
Cuts at: 1132 1229 3178 3541 3559 4934 6479 1132
Size: 97 1949 363 18 1375 1545 2229
Fragments arranged by size:
2229 1949 1545 1375 363 97 18
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7576
PciI A'CATG_T
Cuts at: 3555 6475 3555
Size: 2920 4656
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7576
PfoI T'CCnGG_A
Cuts at: 2716 3880 2716
Size: 1164 6412
PmeI GTTT'AAAC
Cuts at: 4880 4880
Size: 7576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5645 5677 7158 7190 5645
Size: 32 1481 32 6031
Fragments arranged by size:
6031 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7576
PsiI TTA'TAA
Cuts at: 5146 5146
Size: 7576
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 7544
PstI C_TGCA'G
Cuts at: 830 4928 830
Size: 4098 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7576
PvuII CAG'CTG
Cuts at: 2972 3326 2972
Size: 354 7222
SalI G'TCGA_C
Cuts at: 1936 4918 1936
Size: 2982 4594
SbfI CC_TGCA'GG
Cuts at: 4928 4928
Size: 7576
ScaI AGT'ACT
Cuts at: 5824 7536 5824
Size: 1712 5864
SfcI C'TryA_G
Cuts at: 826 1045 3621 4036 4924 6059 6740 6931 826
Size: 219 2576 415 888 1135 681 191 1471
Fragments arranged by size:
2576 1471 1135 888 681 415 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
SgrAI Cr'CCGG_yG
Cuts at: 1700 3762 1700
Size: 2062 5514
SmaI CCC'GGG
Cuts at: 1872 4903 1872
Size: 3031 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2338 3040 3862 4022 4851 5680
Size: 197 926 395 702 822 160 829 829
Cuts at: 5680 6581 6843 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 829 829 822 702 395
262 197 160
SnaBI TAC'GTA
Cuts at: 493 5367 493
Size: 4874 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7576
SphI G_CATG'C
Cuts at: 1132 4934 1132
Size: 3802 3774
SspI AAT'ATT
Cuts at: 5 52 2091 2902 4315 5500 5
Size: 47 2039 811 1413 1185 2081
Fragments arranged by size:
2081 2039 1413 1185 811 47
StuI AGG'CCT
Cuts at: 1517 2870 1517
Size: 1353 6223
StyI C'CwwG_G
Cuts at: 513 1065 2047 5015 513
Size: 552 982 2968 3074
Fragments arranged by size:
3074 2968 982 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 5603 5620 5773 5958 6403 325
Size: 1255 534 3489 17 153 185 445 1498
Fragments arranged by size:
3489 1498 1255 534 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3679 3757 5822
Size: 274 80 33 51 1443 1702 78 2065
Cuts at: 5822 7534 96
Size: 1712 138
Fragments arranged by size:
2065 1712 1702 1443 274 138 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2830 2862 7536 7568 2830
Size: 32 4674 32 2838
Fragments arranged by size:
4674 2838 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7576
XbaI T'CTAG_A
Cuts at: 2962 4912 2962
Size: 1950 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7576
XmaI C'CCGG_G
Cuts at: 1870 4901 1870
Size: 3031 4545
XmnI GAAnn'nnTTC
Cuts at: 2388 4511 4695 5705 2388
Size: 2123 184 1010 4259
Fragments arranged by size:
4259 2123 1010 184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5384 276
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI EcoO109I FseI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI