Restriction map for
pFN21AA0711
(Circular) (Six-base) MAPSORT of: pFN21AA0711.seq Check: 1012 from: 1 to: 6574
pFN21AA0711 6574 bp
With 182 enzymes: SgfI *
December 8, 2014 13:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3374 3374
Size: 6574
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3804 4384 278
Size: 53 83 186 936 2268 580 2468
Fragments arranged by size:
2468 2268 936 580 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2985 3917 1937
Size: 1048 932 4594
Fragments arranged by size:
4594 1048 932
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2116 4637 6021 6289 1632
Size: 484 2521 1384 268 1917
Fragments arranged by size:
2521 1917 1384 484 268
AfeI AGC'GCT
Cuts at: 3284 3476 3284
Size: 192 6382
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 3384 5473 6450 1203
Size: 2181 2089 977 1327
Fragments arranged by size:
2181 2089 1327 977
AgeI A'CCGG_T
Cuts at: 5405 5405
Size: 6574
AhdI GACnn_n'nnGTC
Cuts at: 2356 5303 2356
Size: 2947 3627
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6574
AlwNI CAG_nnn'CTG
Cuts at: 2045 2746 4317 5889 2045
Size: 701 1571 1572 2730
Fragments arranged by size:
2730 1572 1571 701
ApaI G_GGCC'C
Cuts at: 3119 3313 3119
Size: 194 6380
ApaLI G'TGCA_C
Cuts at: 4631 5787 4631
Size: 1156 5418
ApoI r'AATT_y
Cuts at: 1493 3883 4109 4261 6370 1493
Size: 2390 226 152 2109 1697
Fragments arranged by size:
2390 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 3042 3042
Size: 6574
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 2363 2372 2395 2906 2950 3035
Size: 73 108 312 9 23 511 44 85
Cuts at: 3035 3330 3732 3852 3899 1870
Size: 295 402 120 47 4545
Fragments arranged by size:
4545 511 402 312 295 120 108 85
73 47 44 23 9
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2255 2317 2448 2657
Size: 325 217 627 294 174 62 131 209
Cuts at: 2657 3106 3319 3335 3683 3710 3788 3866 3895
Size: 449 213 16 348 27 78 78 29
Cuts at: 3895 5350 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 449 348 325 294 217
213 209 174 131 78 78 62 29
27 16
BanII G_rGCy'C
Cuts at: 1391 2052 2277 2313 2373 2382 2814 2948 3119
Size: 661 225 36 60 9 432 134 171
Cuts at: 3119 3313 3398 3611 3893 1391
Size: 194 85 213 282 4072
Fragments arranged by size:
4072 661 432 282 225 213 194 171
134 85 60 36 9
BbeI G_GCGC'C
Cuts at: 2085 2259 2321 2452 2661 3110 3323 3339 3687
Size: 174 62 131 209 449 213 16 348
Cuts at: 3687 3792 3870 2085
Size: 105 78 4789
Fragments arranged by size:
4789 449 348 213 209 174 131 105
78 62 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3142 928
Size: 971 1243 4360
Fragments arranged by size:
4360 1243 971
BbvCI CC'TCA_GC
Cuts at: 2170 2170
Size: 6574
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3570 3604 4765 4799 1903
Size: 34 1633 34 1161 34 3678
Fragments arranged by size:
3678 1633 1161 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2835 3123
Size: 107 122 71 631 605 502 661 288
Cuts at: 3123 3317 3636 3648 3708 3768 5185 136
Size: 194 319 12 60 60 1417 1525
Fragments arranged by size:
1525 1417 661 631 605 502 319 288
194 122 107 71 60 60 12
BglII A'GATC_T
Cuts at: 3378 6569 3378
Size: 3191 3383
BlpI GC'TnA_GC
Cuts at: 2696 3272 3991 2696
Size: 576 719 5279
Fragments arranged by size:
5279 719 576
Bme1580I G_kGCm'C
Cuts at: 1790 2303 3119 3313 3455 3713 4635 5791 1790
Size: 513 816 194 142 258 922 1156 2573
Fragments arranged by size:
2573 1156 922 816 513 258 194 142
BmgBI CAC'GTC
Cuts at: 1318 2521 1318
Size: 1203 5371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4305 5263 442
Size: 416 53 83 399 24 2888 958 1753
Fragments arranged by size:
2888 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2188 2456 2816 3199 5234 1239
Size: 949 268 360 383 2035 2579
Fragments arranged by size:
2579 2035 949 383 360 268
Bpu10I CC'TnA_GC
Cuts at: 2170 2723 2170
Size: 553 6021
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2366 3401 4699 5564 5862 2366
Size: 1035 1298 865 298 3078
Fragments arranged by size:
3078 1298 1035 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5237 882
Size: 617 3738 2219
Fragments arranged by size:
3738 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3385 4365 493
Size: 711 2181 980 2702
Fragments arranged by size:
2702 2181 980 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2256 2318 2449
Size: 53 83 186 936 549 174 62 131
Cuts at: 2449 2574 2658 2679 2763 3107 3320 3336 3684
Size: 125 84 21 84 344 213 16 348
Cuts at: 3684 3789 3801 3867 4381 4763 5398 275
Size: 105 12 66 514 382 635 1451
Fragments arranged by size:
1451 936 635 549 514 382 348 344
213 186 174 131 125 105 84 84
83 66 62 53 21 16 12
BsaWI w'CCGG_w
Cuts at: 5007 5405 5679 5826 6237 5007
Size: 398 274 147 411 5344
Fragments arranged by size:
5344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2264 2282 2294
Size: 30 165 30 816 30 15 18 12
Cuts at: 2294 2312 3440 3470 1178
Size: 18 1128 30 4282
Fragments arranged by size:
4282 1128 816 165 30 30 30 30
18 18 15 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2303 2381 2555 1186
Size: 1099 18 78 174 5205
Fragments arranged by size:
5205 1099 174 78 18
BseYI C'CCAG_C
Cuts at: 3306 3784 5777 6273 3306
Size: 478 1993 496 3607
Fragments arranged by size:
3607 1993 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 3478 1163
Size: 573 816 926 4259
Fragments arranged by size:
4259 926 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 2881 2982 3006 3127 3304
Size: 275 186 826 54 101 24 121 177
Cuts at: 3304 3483 4024 4785 5813 1540
Size: 179 541 761 1028 2301
Fragments arranged by size:
2301 1028 826 761 541 275 186 179
177 121 101 54 24
BsiHKAI G_wGCw'C
Cuts at: 2013 2277 3816 4635 4720 5791 2013
Size: 264 1539 819 85 1071 2796
Fragments arranged by size:
2796 1539 1071 819 264 85
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2052 2277 2303 2313 2373 2382
Size: 399 223 39 225 26 10 60 9
Cuts at: 2382 2814 2948 3119 3313 3398 3455 3611 3713
Size: 432 134 171 194 85 57 156 102
Cuts at: 3713 3816 3893 4635 4720 5791 1391
Size: 103 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 432 399 225 223 194
171 156 134 103 102 85 85 77
60 57 39 26 10 9
BspEI T'CCGG_A
Cuts at: 6237 6237
Size: 6574
BspHI T'CATG_A
Cuts at: 2778 4463 6193 2778
Size: 1685 1730 3159
Fragments arranged by size:
3159 1730 1685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2859 3374 3929 844
Size: 2015 515 555 3489
Fragments arranged by size:
3489 2015 555 515
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 2845 3528 3564 4461 1394
Size: 429 407 615 683 36 897 3507
Fragments arranged by size:
3507 897 683 615 429 407 36
BsrDI GCAATG_nn'
Cuts at: 65 1248 5069 5243 65
Size: 1183 3821 174 1396
Fragments arranged by size:
3821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2243 2451 2630 2876 2911 2934 3046
Size: 110 433 208 179 246 35 23 112
Cuts at: 3046 3746 5218 5405 1700
Size: 700 1472 187 2869
Fragments arranged by size:
2869 1472 700 433 246 208 187 179
112 110 35 23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 2293 2458 2460 2483 2502 2504 2506 2634
Size: 368 165 2 23 19 2 2 128
Cuts at: 2634 2636 2668 2872 2923 2966 3042 3129 3131
Size: 2 32 204 51 43 76 87 2
Cuts at: 3131 3206 3295 1925
Size: 75 89 5204
Fragments arranged by size:
5204 368 204 165 128 89 87 76
75 51 43 32 23 19 2 2
2 2 2
BssSI C'ACGA_G
Cuts at: 4634 5646 4634
Size: 1012 5562
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6574
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 3239 1184
Size: 2055 4519
BstYI r'GATC_y
Cuts at: 1972 3378 3904 4271 4656 4673 6125 6211 6223
Size: 1406 526 367 385 17 1452 86 12
Cuts at: 6223 6569 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1406 526 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2286 2286
Size: 6574
BtgI C'CryG_G
Cuts at: 513 1065 2004 2216 2313 2682 2898 3111 3168
Size: 552 939 212 97 369 216 213 57
Cuts at: 3168 3252 3315 3345 3370 3428 3535 3645 513
Size: 84 63 30 25 58 107 110 3442
Fragments arranged by size:
3442 939 552 369 216 213 212 110
107 97 84 63 58 57 30 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 2240 3586 3608 4090 4884 4904 2240
Size: 1346 22 482 794 20 3910
Fragments arranged by size:
3910 1346 794 482 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DraIII CAC_nnn'GTG
Cuts at: 2017 2017
Size: 6574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2981 5581 809
Size: 507 1665 2600 1802
Fragments arranged by size:
2600 1802 1665 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2616 2628
Size: 54 1425 50 275 18 357 429 12
Cuts at: 2628 2703 2730 2824 2878 2895 2979 3003 3124
Size: 75 27 94 54 17 84 24 121
Cuts at: 3124 3165 3480 3594 4021 4910 6539 8
Size: 41 315 114 427 889 1629 43
Fragments arranged by size:
1629 1425 889 429 427 357 315 275
121 114 94 84 75 54 54 50
43 41 27 24 18 17 12
EagI C'GGCC_G
Cuts at: 1537 1812 2824 2878 2979 3003 3124 3480 4021
Size: 275 1012 54 101 24 121 356 541
Cuts at: 4021 1537
Size: 4090
Fragments arranged by size:
4090 1012 541 356 275 121 101 54
24
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2301 3092 3407 4504 1353
Size: 786 162 791 315 1097 3423
Fragments arranged by size:
3423 1097 791 786 315 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2192 5161 5535 5681 387
Size: 1064 138 603 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 603 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2275
Size: 6574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2188 2456 2816 3199 4637 5234
Size: 393 484 72 268 360 383 1438 597
Cuts at: 5234 6021 6289 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1438 787 597 484 393 383 360
268 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 3200 3200
Size: 6574
EcoO109I rG'GnC_Cy
Cuts at: 2350 2719 2848 3116 3192 3310 2350
Size: 369 129 268 76 118 5614
Fragments arranged by size:
5614 369 268 129 118 76
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2085 2259 2321 2452 2555 2661 2906 3110
Size: 407 174 62 131 103 106 245 204
Cuts at: 3110 3186 3286 3323 3339 3478 3687 3721 3785
Size: 76 100 37 16 139 209 34 64
Cuts at: 3785 3792 3870 5721 1678
Size: 7 78 1851 2531
Fragments arranged by size:
2531 1851 407 245 209 204 174 139
131 106 103 100 78 76 64 62
37 34 16 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 2342 2374 5215
Size: 32 135 32 692 32 46 32 2841
Cuts at: 5215 5247 5289 5321 1373
Size: 32 42 32 2626
Fragments arranged by size:
2841 2626 692 135 46 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3918 4164 669
Size: 988 281 1980 246 3079
Fragments arranged by size:
3079 1980 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KasI G'GCGC_C
Cuts at: 2081 2255 2317 2448 2657 3106 3319 3335 3683
Size: 174 62 131 209 449 213 16 348
Cuts at: 3683 3788 3866 2081
Size: 105 78 4789
Fragments arranged by size:
4789 449 348 213 209 174 131 105
78 62 16
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3165 5688 5872 1305
Size: 1860 2523 184 2007
Fragments arranged by size:
2523 2007 1860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2705 3596 10
Size: 54 1425 343 873 891 2988
Fragments arranged by size:
2988 1425 891 873 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4532 4891 5050 518
Size: 648 3366 359 159 2042
Fragments arranged by size:
3366 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2174 2208 2218 2315 2583 2650 2695 2743
Size: 568 34 10 97 268 67 45 48
Cuts at: 2743 2900 3113 3177 3217 3306 3325 3430 3537
Size: 157 213 64 40 89 19 105 107
Cuts at: 3537 3666 3990 4667 5815 6060 1606
Size: 129 324 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 568 324 268 245 213
157 129 107 105 97 89 67 64
48 45 40 34 19 10
NaeI GCC'GGC
Cuts at: 2245 2453 2632 2878 2913 2936 3048 3748 2245
Size: 208 179 246 35 23 112 700 5071
Fragments arranged by size:
5071 700 246 208 179 112 35 23
NarI GG'CG_CC
Cuts at: 2082 2256 2318 2449 2658 3107 3320 3336 3684
Size: 174 62 131 209 449 213 16 348
Cuts at: 3684 3789 3867 2082
Size: 105 78 4789
Fragments arranged by size:
4789 449 348 213 209 174 131 105
78 62 16
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5083
Fragments arranged by size:
5083 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NgoMIV G'CCGG_C
Cuts at: 2243 2451 2630 2876 2911 2934 3046 3746 2243
Size: 208 179 246 35 23 112 700 5071
Fragments arranged by size:
5071 700 246 208 179 112 35 23
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 3932 5477 1132
Size: 97 2703 1545 2229
Fragments arranged by size:
2703 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2039 1348
Size: 691 5883
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2794 2893 3592 3603 1108
Size: 1686 99 699 11 4079
Fragments arranged by size:
4079 1686 699 99 11
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2350 2719 2848 2350
Size: 369 129 6076
Fragments arranged by size:
6076 369 129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PspOMI G'GGCC_C
Cuts at: 3115 3309 3115
Size: 194 6380
PstI C_TGCA'G
Cuts at: 830 2111 2649 3657 3926 830
Size: 1281 538 1008 269 3478
Fragments arranged by size:
3478 1281 1008 538 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2650 2695 3177 3217 2650
Size: 45 482 40 6007
Fragments arranged by size:
6007 482 45 40
SacI G_AGCT'C
Cuts at: 2277 2277
Size: 6574
SacII CC_GC'GG
Cuts at: 2219 2316 2901 3114 3431 3538 2219
Size: 97 585 213 317 107 5255
Fragments arranged by size:
5255 585 317 213 107 97
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SapI GCTCTTCn'nnn_
Cuts at: 2301 2301
Size: 6574
SbfI CC_TGCA'GG
Cuts at: 3926 3926
Size: 6574
ScaI AGT'ACT
Cuts at: 4822 6534 4822
Size: 1712 4862
SexAI A'CCwGG_T
Cuts at: 2427 3144 2427
Size: 717 5857
SfcI C'TryA_G
Cuts at: 826 1045 2107 2578 2645 3653 3922 5057 5738
Size: 219 1062 471 67 1008 269 1135 681
Cuts at: 5738 5929 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1062 1008 681 471 269 219
191 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 3123 3648 3123
Size: 525 6049
SfoI GGC'GCC
Cuts at: 2083 2257 2319 2450 2659 3108 3321 3337 3685
Size: 174 62 131 209 449 213 16 348
Cuts at: 3685 3790 3868 2083
Size: 105 78 4789
Fragments arranged by size:
4789 449 348 213 209 174 131 105
78 62 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 3046 1700
Size: 1346 5228
SmaI CCC'GGG
Cuts at: 1872 2053 2374 2397 3332 3734 3901 1872
Size: 181 321 23 935 402 167 4545
Fragments arranged by size:
4545 935 402 321 181 167 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 3416 4678 5579 5841 820
Size: 197 926 402 1071 1262 901 262 1553
Fragments arranged by size:
1553 1262 1071 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 3932 1132
Size: 2800 3774
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6574
StyI C'CwwG_G
Cuts at: 513 1065 2004 3795 4013 513
Size: 552 939 1791 218 3074
Fragments arranged by size:
3074 1791 939 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4601 4618 4771 4956 5401 325
Size: 1255 3021 17 153 185 445 1498
Fragments arranged by size:
3021 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4820 6532 96
Size: 274 80 33 51 1443 2843 1712 138
Fragments arranged by size:
2843 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 2282 2314 3440 3472 6534 6566 2219
Size: 32 31 32 1126 32 3062 32 2227
Fragments arranged by size:
3062 2227 1126 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 3146 1316
Size: 1830 4744
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 6574
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 2051 2372 2395 3330 3732 3899 1870
Size: 181 321 23 935 402 167 4545
Fragments arranged by size:
4545 935 402 321 181 167 23
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3802 4382 276
Size: 53 83 186 936 2268 580 2468
Fragments arranged by size:
2468 2268 936 580 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI
BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SanDI SrfI SwaI