Restriction map for
pFN21AA0709
(Circular) (Six-base) MAPSORT of: pFN21AA0709.seq Check: 9262 from: 1 to: 9142
pFN21AA0709 9142 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2055 5964 2055
Size: 3909 5233
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6952 278
Size: 53 83 186 936 5416 2468
Fragments arranged by size:
5416 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 6485 1937
Size: 592 3956 4594
Fragments arranged by size:
4594 3956 592
Acc65I G'GTAC_C
Cuts at: 2270 6463 2270
Size: 4193 4949
AclI AA'CG_TT
Cuts at: 1563 7269 7642 1563
Size: 5706 373 3063
Fragments arranged by size:
5706 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2128 4687 5408 7205 8589 8857 1632
Size: 496 2559 721 1797 1384 268 1917
Fragments arranged by size:
2559 1917 1797 1384 721 496 268
AfeI AGC'GCT
Cuts at: 2231 2675 5367 5885 6225 2231
Size: 444 2692 518 340 5148
Fragments arranged by size:
5148 2692 518 444 340
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8945
AflIII A'CryG_T
Cuts at: 1203 8041 9018 1203
Size: 6838 977 1327
Fragments arranged by size:
6838 1327 977
AgeI A'CCGG_T
Cuts at: 7973 7973
Size: 9142
AhdI GACnn_n'nnGTC
Cuts at: 2392 7871 2392
Size: 5479 3663
AleI CACnn'nnGTG
Cuts at: 1166 3956 6047 1166
Size: 2790 2091 4261
Fragments arranged by size:
4261 2790 2091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2829 3083 1184
Size: 1645 254 7243
Fragments arranged by size:
7243 1645 254
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4200 4232 4702 4734 4200
Size: 32 470 32 8608
Fragments arranged by size:
8608 470 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2284 3489 3946 4123 4138 5797 6885 8457 2284
Size: 1205 457 177 15 1659 1088 1572 2969
Fragments arranged by size:
2969 1659 1572 1205 1088 457 177 15
ApaI G_GGCC'C
Cuts at: 2013 2413 3464 3925 4132 4651 5751 6072 6086
Size: 400 1051 461 207 519 1100 321 14
Cuts at: 6086 2013
Size: 5069
Fragments arranged by size:
5069 1100 1051 519 461 400 321 207
14
ApaLI G'TGCA_C
Cuts at: 2563 3649 4592 7199 8355 2563
Size: 1086 943 2607 1156 3350
Fragments arranged by size:
3350 2607 1156 1086 943
ApoI r'AATT_y
Cuts at: 1493 3284 3358 6451 6677 6829 8938 1493
Size: 1791 74 3093 226 152 2109 1697
Fragments arranged by size:
3093 2109 1791 1697 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 7696 160
Size: 872 6664 1606
Fragments arranged by size:
6664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9142
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 3031 3836 4211 4644 5380 5532
Size: 73 126 962 805 375 433 736 152
Cuts at: 5532 6073 6467 1870
Size: 541 394 4545
Fragments arranged by size:
4545 962 805 736 541 433 394 375
152 126 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2254 2287 3832 3865 4434 4467 2254
Size: 33 1545 33 569 33 6929
Fragments arranged by size:
6929 1545 569 33 33 33
BamHI G'GATC_C
Cuts at: 4258 4932 6472 6839 4258
Size: 674 1540 367 6561
Fragments arranged by size:
6561 1540 674 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2096 2270 2303 2438
Size: 325 217 627 286 23 174 33 135
Cuts at: 2438 3402 3642 4744 4827 5179 5408 5984 6141
Size: 964 240 1102 83 352 229 576 157
Cuts at: 6141 6318 6350 6463 7918 618
Size: 177 32 113 1455 1842
Fragments arranged by size:
1842 1455 1102 964 627 576 352 325
286 240 229 217 177 174 157 135
113 83 33 32 23
BanII G_rGCy'C
Cuts at: 1391 2013 2413 3111 3417 3464 3925 4132 4461
Size: 622 400 698 306 47 461 207 329
Cuts at: 4461 4651 5193 5354 5394 5582 5751 6072 6086
Size: 190 542 161 40 188 169 321 14
Cuts at: 6086 6461 1391
Size: 375 4072
Fragments arranged by size:
4072 698 622 542 461 400 375 329
321 306 207 190 188 169 161 47
40 14
BbeI G_GCGC'C
Cuts at: 2100 3646 5988 6145 6322 2100
Size: 1546 2342 157 177 4920
Fragments arranged by size:
4920 2342 1546 177 157
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2125 3621 928
Size: 971 226 1496 6449
Fragments arranged by size:
6449 1496 971 226
BbvCI CC'TCA_GC
Cuts at: 5260 5260
Size: 9142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5868 5902 7333 7367 1903
Size: 34 3931 34 1431 34 3678
Fragments arranged by size:
3931 3678 1431 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7034 8244 7034
Size: 1210 7932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2094 3160
Size: 107 122 71 631 605 345 77 1066
Cuts at: 3160 3468 3852 4002 4109 4476 5177 5331 5483
Size: 308 384 150 107 367 701 154 152
Cuts at: 5483 7753 136
Size: 2270 1525
Fragments arranged by size:
2270 1525 1066 701 631 605 384 367
345 308 154 152 150 122 107 107
77 71
BglII A'GATC_T
Cuts at: 4191 9137 4191
Size: 4946 4196
BlpI GC'TnA_GC
Cuts at: 2825 3533 4139 6095 6198 6559 2825
Size: 708 606 1956 103 361 5408
Fragments arranged by size:
5408 1956 708 606 361 103
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2291 2413 2567 3464 3653 3925 3953
Size: 223 278 122 154 897 189 272 28
Cuts at: 3953 4132 4596 4651 4747 5182 5336 5387 5496
Size: 179 464 55 96 435 154 51 109
Cuts at: 5496 5751 6072 6086 6355 7203 8359 1790
Size: 255 321 14 269 848 1156 2573
Fragments arranged by size:
2573 1156 897 848 464 435 321 278
272 269 255 223 189 179 154 154
122 109 96 55 51 28 14
BmgBI CAC'GTC
Cuts at: 1318 3582 4450 1318
Size: 2264 868 6010
Fragments arranged by size:
6010 2264 868
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2390 2883 2977
Size: 416 53 83 399 24 973 493 94
Cuts at: 2977 2988 3736 4822 5185 5208 6073 6873 7831
Size: 11 748 1086 363 23 865 800 958
Cuts at: 7831 442
Size: 1753
Fragments arranged by size:
1753 1086 973 958 865 800 748 493
416 399 363 94 83 53 24 23
11
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9142
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4549 4603 5596 4549
Size: 54 993 8095
Fragments arranged by size:
8095 993 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 3102 3458 3939 4306 6358 7802 1239
Size: 950 913 356 481 367 2052 1444 2579
Fragments arranged by size:
2579 2052 1444 950 913 481 367 356
Bpu10I CC'TnA_GC
Cuts at: 4619 5260 5348 4619
Size: 641 88 8413
Fragments arranged by size:
8413 641 88
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2939 4458 7267 8132 8430 2465
Size: 474 1519 2809 865 298 3177
Fragments arranged by size:
3177 2809 1519 865 474 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2248 2705 5300 5605 7805 882
Size: 617 749 457 2595 305 2200 2219
Fragments arranged by size:
2595 2219 2200 749 617 457 305
BsaAI yAC'GTr
Cuts at: 493 1204 4539 4566 5491 5623 5671 6933 493
Size: 711 3335 27 925 132 48 1262 2702
Fragments arranged by size:
3335 2702 1262 925 711 132 48 27
BsaBI GATnn'nnATC
Cuts at: 9136 9136
Size: 9142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2109 3643 3962
Size: 53 83 186 936 564 12 1534 319
Cuts at: 3962 5985 6142 6319 6949 7331 7966 275
Size: 2023 157 177 630 382 635 1451
Fragments arranged by size:
2023 1534 1451 936 635 630 564 382
319 186 177 157 83 53 12
BsaWI w'CCGG_w
Cuts at: 4041 4323 6312 7575 7973 8247 8394 8805 4041
Size: 282 1989 1263 398 274 147 411 4378
Fragments arranged by size:
4378 1989 1263 411 398 282 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3308 3338 3722 3752 4361
Size: 30 165 30 1905 30 384 30 609
Cuts at: 4361 4391 4496 4526 4721 4751 5924 5954 1178
Size: 30 105 30 195 30 1173 30 4366
Fragments arranged by size:
4366 1905 1173 609 384 195 165 105
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2510 2858 3014 3332 3566 4172 4175
Size: 871 453 348 156 318 234 606 3
Cuts at: 4175 5318 5588 5621 5745 1186
Size: 1143 270 33 124 4583
Fragments arranged by size:
4583 1143 871 606 453 348 318 270
234 156 124 33 3
BseYI C'CCAG_C
Cuts at: 2226 2676 3607 3992 4020 4092 4123 4131 5031
Size: 450 931 385 28 72 31 8 900
Cuts at: 5031 5280 5359 5860 5992 6086 6238 8345 8841
Size: 249 79 501 132 94 152 2107 496
Cuts at: 8841 2226
Size: 2527
Fragments arranged by size:
2527 2107 931 900 501 496 450 385
249 152 132 94 79 72 31 28
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2063 2590 4546 4661 5234 5281 1163
Size: 573 327 527 1956 115 573 47 5024
Fragments arranged by size:
5024 1956 573 573 527 327 115 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2021 2089 3105 4324 5290
Size: 275 186 16 4 68 1016 1219 966
Cuts at: 5290 6592 7353 8381 1540
Size: 1302 761 1028 2301
Fragments arranged by size:
2301 1302 1219 1028 1016 966 761 275
186 68 16 4
BsiHKAI G_wGCw'C
Cuts at: 2512 2567 3653 4167 4530 4596 5394 5832 5955
Size: 55 1086 514 363 66 798 438 123
Cuts at: 5955 7203 7288 8359 2512
Size: 1248 85 1071 3295
Fragments arranged by size:
3295 1248 1086 1071 798 514 438 363
123 85 66 55
BsmI GAATG_Cn'
Cuts at: 5066 6653 6746 5066
Size: 1587 93 7462
Fragments arranged by size:
7462 1587 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2291 2413 2512 2567 3111 3417
Size: 399 223 278 122 99 55 544 306
Cuts at: 3417 3464 3653 3925 3953 4132 4167 4461 4530
Size: 47 189 272 28 179 35 294 69
Cuts at: 4530 4596 4651 4747 5182 5193 5336 5354 5387
Size: 66 55 96 435 11 143 18 33
Cuts at: 5387 5394 5496 5582 5751 5832 5955 6072 6086
Size: 7 102 86 169 81 123 117 14
Cuts at: 6086 6355 6461 7203 7288 8359 1391
Size: 269 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 435 399 306 294
278 272 269 223 189 179 169 143
123 122 117 106 102 99 96 86
85 81 69 66 55 55 47 35
33 28 18 14 11 7
BspEI T'CCGG_A
Cuts at: 8805 8805
Size: 9142
BspHI T'CATG_A
Cuts at: 7031 8761 7031
Size: 1730 7412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2055 2176 4902 5964 6497 844
Size: 1211 121 2726 1062 533 3489
Fragments arranged by size:
3489 2726 1211 1062 533 121
BsrBI CCG'CTC
Cuts at: 1394 1823 2922 4072 5551 5897 7029 1394
Size: 429 1099 1150 1479 346 1132 3507
Fragments arranged by size:
3507 1479 1150 1132 1099 429 346
BsrDI GCAATG_nn'
Cuts at: 65 1248 7637 7811 65
Size: 1183 6389 174 1396
Fragments arranged by size:
6389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2016 2020 2253 3773 3936 4329 7786
Size: 110 206 4 233 1520 163 393 3457
Cuts at: 7786 7973 1700
Size: 187 2869
Fragments arranged by size:
3457 2869 1520 393 233 206 187 163
110 4
BsrGI T'GTAC_A
Cuts at: 96 3810 4788 96
Size: 3714 978 4450
Fragments arranged by size:
4450 3714 978
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9142
BssSI C'ACGA_G
Cuts at: 2522 2843 2917 4220 6192 7202 8214 2522
Size: 321 74 1303 1972 1010 1012 3450
Fragments arranged by size:
3450 1972 1303 1012 1010 321 74
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9142
BstBI TT'CG_AA
Cuts at: 7946 7946
Size: 9142
BstEII G'GTnAC_C
Cuts at: 1184 2384 2453 2630 3673 5038 1184
Size: 1200 69 177 1043 1365 5288
Fragments arranged by size:
5288 1365 1200 1043 177 69
BstXI CCAn_nnnn'nTGG
Cuts at: 3126 5273 3126
Size: 2147 6995
BstYI r'GATC_y
Cuts at: 1972 2818 2838 3982 4191 4215 4258 4422 4431
Size: 846 20 1144 209 24 43 164 9
Cuts at: 4431 4932 6472 6839 7224 7241 8693 8779 8791
Size: 501 1540 367 385 17 1452 86 12
Cuts at: 8791 9137 1972
Size: 346 1977
Fragments arranged by size:
1977 1540 1452 1144 846 501 385 367
346 209 164 86 43 24 20 17
12 9
BtgI C'CryG_G
Cuts at: 513 1065 2004 2149 2313 2551 2599 3099 3559
Size: 552 939 145 164 238 48 500 460
Cuts at: 3559 3658 3847 3882 5311 5776 6168 513
Size: 99 189 35 1429 465 392 3487
Fragments arranged by size:
3487 1429 939 552 500 465 460 392
238 189 164 145 99 48 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3046 4707 487
Size: 683 56 1820 1661 4922
Fragments arranged by size:
4922 1820 1661 683 56
BtsI GCAGTG_nn'
Cuts at: 2285 2473 2939 3178 4391 4406 5749 5830 6658
Size: 188 466 239 1213 15 1343 81 828
Cuts at: 6658 7452 7472 2285
Size: 794 20 3955
Fragments arranged by size:
3955 1343 1213 828 794 466 239 188
81 20 15
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9107
DraI TTT'AAA
Cuts at: 6446 6446
Size: 9142
DraIII CAC_nnn'GTG
Cuts at: 2384 2892 4171 2384
Size: 508 1279 7355
Fragments arranged by size:
7355 1279 508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8149 809
Size: 507 6833 1802
Fragments arranged by size:
6833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2018 2086
Size: 54 1425 50 275 18 184 4 68
Cuts at: 2086 2297 2470 3102 3867 4146 4173 4392 5275
Size: 211 173 632 765 279 27 219 883
Cuts at: 5275 6589 7478 9107 8
Size: 1314 889 1629 43
Fragments arranged by size:
1629 1425 1314 889 883 765 632 279
275 219 211 184 173 68 54 50
43 27 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2014 2018 2086 3102 6589 1537
Size: 275 202 4 68 1016 3487 4090
Fragments arranged by size:
4090 3487 1016 275 202 68 4
EarI CTCTTCn'nnn_
Cuts at: 1353 4082 7072 1353
Size: 2729 2990 3423
Fragments arranged by size:
3423 2990 2729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3290 4619 7729 8103 8249 387
Size: 1064 138 1701 1329 3110 374 146 1280
Fragments arranged by size:
3110 1701 1329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5392 5392
Size: 9142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2128 2189 3102 3458 3939 4306 4687
Size: 393 496 61 913 356 481 367 381
Cuts at: 4687 5408 6358 7205 7802 8589 8857 1239
Size: 721 950 847 597 787 268 1524
Fragments arranged by size:
1524 950 913 847 787 721 597 496
481 393 381 367 356 268 61
EcoNI CCTnn'n_nnAGG
Cuts at: 5128 5522 5128
Size: 394 8748
EcoO109I rG'GnC_Cy
Cuts at: 2009 2409 2500 3460 3760 4110 4128 5685 5748
Size: 400 91 960 300 350 18 1557 63
Cuts at: 5748 6068 6069 6083 6339 2009
Size: 320 1 14 256 4812
Fragments arranged by size:
4812 1557 960 400 350 320 300 256
91 63 18 14 1
EcoRI G'AATT_C
Cuts at: 3284 6451 6829 3284
Size: 3167 378 5597
Fragments arranged by size:
5597 3167 378
FseI GG_CCGG'CC
Cuts at: 2020 2024 2020
Size: 4 9138
FspI TGC'GCA
Cuts at: 5543 7648 5543
Size: 2105 7037
HaeII r_GCGC'y
Cuts at: 1678 2100 2233 2677 3646 3866 3882 4285 4702
Size: 422 133 444 969 220 16 403 417
Cuts at: 4702 5369 5887 5988 6145 6153 6227 6322 8289
Size: 667 518 101 157 8 74 95 1967
Cuts at: 8289 1678
Size: 2531
Fragments arranged by size:
2531 1967 969 667 518 444 422 417
403 220 157 133 101 95 74 16
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 3647 3679 4361
Size: 32 135 32 806 32 1237 32 682
Cuts at: 4361 4393 7783 7815 7857 7889 1373
Size: 32 3390 32 42 32 2626
Fragments arranged by size:
3390 2626 1237 806 682 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6486 6732 669
Size: 988 281 4548 246 3079
Fragments arranged by size:
4548 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6185 748
Size: 5437 3705
HpaI GTT'AAC
Cuts at: 6732 6732
Size: 9142
KasI G'GCGC_C
Cuts at: 2096 3642 5984 6141 6318 2096
Size: 1546 2342 157 177 4920
Fragments arranged by size:
4920 2342 1546 177 157
KpnI G_GTAC'C
Cuts at: 2274 6467 2274
Size: 4193 4949
MfeI C'AATT_G
Cuts at: 6741 6741
Size: 9142
MluI A'CGCG_T
Cuts at: 9018 9018
Size: 9142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2919 3557 6084 8256 8440 1305
Size: 1614 638 2527 2172 184 2007
Fragments arranged by size:
2527 2172 2007 1614 638 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2299 2472 3869 4148 4175
Size: 54 1425 343 467 173 1397 279 27
Cuts at: 4175 4394 10
Size: 219 4758
Fragments arranged by size:
4758 1425 1397 467 343 279 219 173
54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 3956 6047 6167 6407 6422 7100 7459
Size: 648 2790 2091 120 240 15 678 359
Cuts at: 7459 7618 518
Size: 159 2042
Fragments arranged by size:
2790 2091 2042 678 648 359 240 159
120 15
MspA1I CmG'CkG
Cuts at: 1606 2392 2734 2745 2799 3210 3474 3532 3670
Size: 786 342 11 54 411 264 58 138
Cuts at: 3670 4135 4414 5035 5235 5280 5434 5614 5703
Size: 465 279 621 200 45 154 180 89
Cuts at: 5703 5784 6047 6108 6132 6242 6558 7235 8383
Size: 81 263 61 24 110 316 677 1148
Cuts at: 8383 8628 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 786 677 621 465 411 342
316 279 264 263 245 200 180 154
138 110 89 81 61 58 54 45
24 11
NaeI GCC'GGC
Cuts at: 2018 2022 3938 4331 2018
Size: 4 1916 393 6829
Fragments arranged by size:
6829 1916 393 4
NarI GG'CG_CC
Cuts at: 2097 3643 5985 6142 6319 2097
Size: 1546 2342 157 177 4920
Fragments arranged by size:
4920 2342 1546 177 157
NcoI C'CATG_G
Cuts at: 513 1065 2004 2149 3559 3658 3882 5311 5776
Size: 552 939 145 1410 99 224 1429 465
Cuts at: 5776 6168 513
Size: 392 3487
Fragments arranged by size:
3487 1429 1410 939 552 465 392 224
145 99
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9142
NgoMIV G'CCGG_C
Cuts at: 2016 2020 3936 4329 2016
Size: 4 1916 393 6829
Fragments arranged by size:
6829 1916 393 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9142
NotI GC'GGCC_GC
Cuts at: 6589 6589
Size: 9142
NspI r_CATG'y
Cuts at: 1132 1229 2332 3234 4183 6094 6500 8045 1132
Size: 97 1103 902 949 1911 406 1545 2229
Fragments arranged by size:
2229 1911 1545 1103 949 902 406 97
PasI CC'CwG_GG
Cuts at: 1348 2102 2176 2323 4015 4105 4909 5791 1348
Size: 754 74 147 1692 90 804 882 4699
Fragments arranged by size:
4699 1692 882 804 754 147 90 74
PciI A'CATG_T
Cuts at: 8041 8041
Size: 9142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2892 3271 4123 5146 5797 1108
Size: 1784 379 852 1023 651 4453
Fragments arranged by size:
4453 1784 1023 852 651 379
PfoI T'CCnGG_A
Cuts at: 2118 3426 4500 5823 5919 2118
Size: 1308 1074 1323 96 5341
Fragments arranged by size:
5341 1323 1308 1074 96
PmeI GTTT'AAAC
Cuts at: 6446 6446
Size: 9142
PmlI CAC'GTG
Cuts at: 4539 4566 4539
Size: 27 9115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 4502 4534 4991 5023 7211 7243 8724
Size: 32 574 32 457 32 2188 32 1481
Cuts at: 8724 8756 3896
Size: 32 4282
Fragments arranged by size:
4282 2188 1481 574 457 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2500 3760 2500
Size: 1260 7882
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9142
PsiI TTA'TAA
Cuts at: 4727 6712 4727
Size: 1985 7157
PspOMI G'GGCC_C
Cuts at: 2009 2409 3460 3921 4128 4647 5747 6068 6082
Size: 400 1051 461 207 519 1100 321 14
Cuts at: 6082 2009
Size: 5069
Fragments arranged by size:
5069 1100 1051 519 461 400 321 207
14
PstI C_TGCA'G
Cuts at: 830 2160 3059 3195 3354 3759 4080 4386 5721
Size: 1330 899 136 159 405 321 306 1335
Cuts at: 5721 5961 6494 830
Size: 240 533 3478
Fragments arranged by size:
3478 1335 1330 899 533 405 321 306
240 159 136
PvuI CG_AT'CG
Cuts at: 2001 5290 2001
Size: 3289 5853
PvuII CAG'CTG
Cuts at: 2392 2734 2745 2799 3210 3532 3670 4135 4414
Size: 342 11 54 411 322 138 465 279
Cuts at: 4414 5035 5235 5703 5784 6047 6108 2392
Size: 621 200 468 81 263 61 5426
Fragments arranged by size:
5426 621 468 465 411 342 322 279
263 200 138 81 61 54 11
SacI G_AGCT'C
Cuts at: 5394 5394
Size: 9142
SalI G'TCGA_C
Cuts at: 1936 6484 1936
Size: 4548 4594
SapI GCTCTTCn'nnn_
Cuts at: 4082 4082
Size: 9142
SbfI CC_TGCA'GG
Cuts at: 3195 3759 6494 3195
Size: 564 2735 5843
Fragments arranged by size:
5843 2735 564
ScaI AGT'ACT
Cuts at: 3740 7390 9102 3740
Size: 3650 1712 3780
Fragments arranged by size:
3780 3650 1712
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 9142
SfcI C'TryA_G
Cuts at: 826 1045 2156 3055 3191 3350 3628 3755 4076
Size: 219 1111 899 136 159 278 127 321
Cuts at: 4076 4382 4902 5717 5957 6358 6490 7625 8306
Size: 306 520 815 240 401 132 1135 681
Cuts at: 8306 8497 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1111 899 815 681 520 401
321 306 278 240 219 191 159 136
132 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 2017 3468 4109 2017
Size: 1451 641 7050
Fragments arranged by size:
7050 1451 641
SfoI GGC'GCC
Cuts at: 2098 3644 5986 6143 6320 2098
Size: 1546 2342 157 177 4920
Fragments arranged by size:
4920 2342 1546 177 157
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9142
SmaI CCC'GGG
Cuts at: 1872 3838 4646 5382 6075 6469 1872
Size: 1966 808 736 693 394 4545
Fragments arranged by size:
4545 1966 808 736 693 394
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 2954 4473 7246 8147 8409
Size: 197 926 501 510 1519 2773 901 262
Cuts at: 8409 820
Size: 1553
Fragments arranged by size:
2773 1553 1519 926 901 510 501 262
197
SnaBI TAC'GTA
Cuts at: 493 5671 6933 493
Size: 5178 1262 2702
Fragments arranged by size:
5178 2702 1262
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9142
SphI G_CATG'C
Cuts at: 1132 6094 6500 1132
Size: 4962 406 3774
Fragments arranged by size:
4962 3774 406
SspI AAT'ATT
Cuts at: 5 52 7066 5
Size: 47 7014 2081
Fragments arranged by size:
7014 2081 47
StuI AGG'CCT
Cuts at: 1517 2789 3701 5513 1517
Size: 1272 912 1812 5146
Fragments arranged by size:
5146 1812 1272 912
StyI C'CwwG_G
Cuts at: 513 1065 2004 2149 3559 3658 3882 5311 5776
Size: 552 939 145 1410 99 224 1429 465
Cuts at: 5776 6168 6581 513
Size: 392 413 3074
Fragments arranged by size:
3074 1429 1410 939 552 465 413 392
224 145 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2372 2666 3410 3830 4378 5134 5522
Size: 1255 792 294 744 420 548 756 388
Cuts at: 5522 6161 7169 7186 7339 7524 7969 325
Size: 639 1008 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1008 792 756 744 639 548
445 420 388 294 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3738 3810 4512
Size: 274 80 33 51 1443 1761 72 702
Cuts at: 4512 4788 7388 9100 96
Size: 276 2600 1712 138
Fragments arranged by size:
2600 1761 1712 1443 702 276 274 138
80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3578 3610 4766 4798 4811 4843 5126
Size: 32 238 32 1156 32 13 32 283
Cuts at: 5126 5158 9102 9134 3308
Size: 32 3944 32 3316
Fragments arranged by size:
3944 3316 1156 283 238 32 32 32
32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 3254 3446 1316
Size: 1938 192 7012
Fragments arranged by size:
7012 1938 192
XbaI T'CTAG_A
Cuts at: 6478 6478
Size: 9142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2320 2465 3139 3600 5239 5897 6166 2281
Size: 39 145 674 461 1639 658 269 5257
Fragments arranged by size:
5257 1639 674 658 461 269 145 39
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9142
XmaI C'CCGG_G
Cuts at: 1870 3836 4644 5380 6073 6467 1870
Size: 1966 808 736 693 394 4545
Fragments arranged by size:
4545 1966 808 736 693 394
XmnI GAAnn'nnTTC
Cuts at: 7271 7271
Size: 9142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6950 276
Size: 53 83 186 936 5416 2468
Fragments arranged by size:
5416 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV
FalI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SanDI SrfI SwaI