Restriction map for
pFN21AA0708
(Circular) (Six-base) MAPSORT of: pFN21AA0708.seq Check: 4528 from: 1 to: 12256
pFN21AA0708 12256 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 8354 8552 8354
Size: 198 12058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10066 278
Size: 53 83 186 936 8530 2468
Fragments arranged by size:
8530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2220 3269 3752 8634 9599 1937
Size: 283 1049 483 4882 965 4594
Fragments arranged by size:
4882 4594 1049 965 483 283
Acc65I G'GTAC_C
Cuts at: 9577 9577
Size: 12256
AclI AA'CG_TT
Cuts at: 1563 10383 10756 1563
Size: 8820 373 3063
Fragments arranged by size:
8820 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2091 2159 2255 2473 2733 3330 4418 6328
Size: 459 68 96 218 260 597 1088 1910
Cuts at: 6328 6710 7406 9290 10319 11703 11971 1632
Size: 382 696 1884 1029 1384 268 1917
Fragments arranged by size:
1917 1910 1884 1384 1088 1029 696 597
459 382 268 260 218 96 68
AfeI AGC'GCT
Cuts at: 2789 3101 5843 8708 2789
Size: 312 2742 2865 6337
Fragments arranged by size:
6337 2865 2742 312
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12059
AflIII A'CryG_T
Cuts at: 1203 2523 4689 5945 11155 12132 1203
Size: 1320 2166 1256 5210 977 1327
Fragments arranged by size:
5210 2166 1327 1320 1256 977
AgeI A'CCGG_T
Cuts at: 11087 11087
Size: 12256
AhdI GACnn_n'nnGTC
Cuts at: 8034 10985 8034
Size: 2951 9305
AleI CACnn'nnGTG
Cuts at: 1166 5637 6639 7491 1166
Size: 4471 1002 852 5931
Fragments arranged by size:
5931 4471 1002 852
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2588 2762 6078 9150 9251 9297 1184
Size: 1404 174 3316 3072 101 46 4143
Fragments arranged by size:
4143 3316 3072 1404 174 101 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 12224
AlwNI CAG_nnn'CTG
Cuts at: 2368 2581 3353 3940 4258 5173 5290 6103 6913
Size: 213 772 587 318 915 117 813 810
Cuts at: 6913 7309 7849 7949 8119 8527 9019 9999 11571
Size: 396 540 100 170 408 492 980 1572
Cuts at: 11571 2368
Size: 3053
Fragments arranged by size:
3053 1572 980 915 813 810 772 587
540 492 408 396 318 213 170 117
100
ApaI G_GGCC'C
Cuts at: 2475 2965 3320 4880 5025 5897 6487 7521 7881
Size: 490 355 1560 145 872 590 1034 360
Cuts at: 7881 2475
Size: 6850
Fragments arranged by size:
6850 1560 1034 872 590 490 360 355
145
ApaLI G'TGCA_C
Cuts at: 3290 8482 8902 10313 11469 3290
Size: 5192 420 1411 1156 4077
Fragments arranged by size:
5192 4077 1411 1156 420
ApoI r'AATT_y
Cuts at: 1493 3107 6058 7172 7346 9565 9791 9943 12052
Size: 1614 2951 1114 174 2219 226 152 2109
Cuts at: 12052 1493
Size: 1697
Fragments arranged by size:
2951 2219 2109 1697 1614 1114 226 174
152
AseI AT'TA_AT
Cuts at: 160 1032 10810 160
Size: 872 9778 1606
Fragments arranged by size:
9778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12256
AvaI C'yCGr_G
Cuts at: 1870 1943 2307 4525 5868 6264 6558 9425 9581
Size: 73 364 2218 1343 396 294 2867 156
Cuts at: 9581 1870
Size: 4545
Fragments arranged by size:
4545 2867 2218 1343 396 364 294 156
73
AvrII C'CTAG_G
Cuts at: 3604 3604
Size: 12256
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3757 3790 3757
Size: 33 12223
BamHI G'GATC_C
Cuts at: 9246 9586 9953 9246
Size: 340 367 11549
Fragments arranged by size:
11549 367 340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 4940 6006 6379 6599
Size: 325 217 627 251 2902 1066 373 220
Cuts at: 6599 6732 8748 9577 11032 618
Size: 133 2016 829 1455 1842
Fragments arranged by size:
2902 2016 1842 1455 1066 829 627 373
325 251 220 217 133
BanII G_rGCy'C
Cuts at: 1391 2475 2506 2611 2965 3320 3879 4110 4532
Size: 1084 31 105 354 355 559 231 422
Cuts at: 4532 4880 5025 5121 5483 5751 5897 6377 6487
Size: 348 145 96 362 268 146 480 110
Cuts at: 6487 6700 7521 7872 7881 8000 8231 9575 1391
Size: 213 821 351 9 119 231 1344 4072
Fragments arranged by size:
4072 1344 1084 821 559 480 422 362
355 354 351 348 268 231 231 213
146 145 119 110 105 96 31 9
BbeI G_GCGC'C
Cuts at: 6010 6383 6603 6010
Size: 373 220 11663
Fragments arranged by size:
11663 373 220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2479 3082 5415 7619 9296 9411 928
Size: 971 580 603 2333 2204 1677 115 3773
Fragments arranged by size:
3773 2333 2204 1677 971 603 580 115
BbvCI CC'TCA_GC
Cuts at: 2972 5051 9218 2972
Size: 2079 4167 6010
Fragments arranged by size:
6010 4167 2079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5918 5952 10447 10481 1903
Size: 34 3981 34 4495 34 3678
Fragments arranged by size:
4495 3981 3678 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10148 11358 10148
Size: 1210 11046
BclI T'GATC_A
Cuts at: 1548 7675 1548
Size: 6127 6129
BfrBI ATG'CAT
Cuts at: 5206 5206
Size: 12256
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2047 3123 4926
Size: 107 122 71 631 605 375 1076 1803
Cuts at: 4926 5139 5853 6098 7876 8523 9332 9430 10867
Size: 213 714 245 1778 647 809 98 1437
Cuts at: 10867 136
Size: 1525
Fragments arranged by size:
1803 1778 1525 1437 1076 809 714 647
631 605 375 245 213 122 107 98
71
BglII A'GATC_T
Cuts at: 3612 3705 4494 12251 3612
Size: 93 789 7757 3617
Fragments arranged by size:
7757 3617 789 93
BlpI GC'TnA_GC
Cuts at: 5283 6434 7284 8076 9673 5283
Size: 1151 850 792 1597 7866
Fragments arranged by size:
7866 1597 1151 850 792
Bme1580I G_kGCm'C
Cuts at: 1790 2043 2257 2475 2965 3294 3320 3389 4880
Size: 253 214 218 490 329 26 69 1491
Cuts at: 4880 5025 5897 5971 6184 6487 7434 7521 7881
Size: 145 872 74 213 303 947 87 360
Cuts at: 7881 8486 8906 8976 9426 10317 11473 1790
Size: 605 420 70 450 891 1156 2573
Fragments arranged by size:
2573 1491 1156 947 891 872 605 490
450 420 360 329 303 253 218 214
213 145 87 74 70 69 26
BmgBI CAC'GTC
Cuts at: 1318 2614 2805 7269 1318
Size: 1296 191 4464 6305
Fragments arranged by size:
6305 4464 1296 191
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3295 5593 6113
Size: 416 53 83 399 24 1878 2298 520
Cuts at: 6113 6418 8110 9987 10945 442
Size: 305 1692 1877 958 1753
Fragments arranged by size:
2298 1878 1877 1753 1692 958 520 416
399 305 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 8610 1056
Size: 7554 4702
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2625 3936 4609 5272 5548 8437 8482 2625
Size: 1311 673 663 276 2889 45 6399
Fragments arranged by size:
6399 2889 1311 673 663 276 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2705 2868 2924 3097 3137 3326 3431 4952
Size: 1466 163 56 173 40 189 105 1521
Cuts at: 4952 5347 5467 5998 5999 6723 6863 6944 7183
Size: 395 120 531 1 724 140 81 239
Cuts at: 7183 7403 7718 7848 9041 9140 9173 10916 1239
Size: 220 315 130 1193 99 33 1743 2579
Fragments arranged by size:
2579 1743 1521 1466 1193 724 531 395
315 239 220 189 173 163 140 130
120 105 99 81 56 40 33 1
Bpu10I CC'TnA_GC
Cuts at: 2538 2972 4797 4848 5051 5898 6324 6821 6914
Size: 434 1825 51 203 847 426 497 93
Cuts at: 6914 8142 8184 9218 2538
Size: 1228 42 1034 5576
Fragments arranged by size:
5576 1825 1228 1034 847 497 434 426
203 93 51 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4943 5668 6259 7639 8786 10381 11246 11544 4943
Size: 725 591 1380 1147 1595 865 298 5655
Fragments arranged by size:
5655 1595 1380 1147 865 725 591 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2844 3578 3890 4653 4808 4970 6215
Size: 617 1345 734 312 763 155 162 1245
Cuts at: 6215 7370 9171 10919 882
Size: 1155 1801 1748 2219
Fragments arranged by size:
2219 1801 1748 1345 1245 1155 763 734
617 312 162 155
BsaAI yAC'GTr
Cuts at: 493 1204 2446 7819 10047 493
Size: 711 1242 5373 2228 2702
Fragments arranged by size:
5373 2702 2228 1242 711
BsaBI GATnn'nnATC
Cuts at: 12250 12250
Size: 12256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6007 6380 6600 10063
Size: 53 83 186 936 4474 373 220 3463
Cuts at: 10063 10445 11080 275
Size: 382 635 1451
Fragments arranged by size:
4474 3463 1451 936 635 382 373 220
186 83 53
BsaWI w'CCGG_w
Cuts at: 6369 8378 10689 11087 11361 11508 11919 6369
Size: 2009 2311 398 274 147 411 6706
Fragments arranged by size:
6706 2311 2009 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2843 2873 3123 3153 3785
Size: 30 165 30 1440 30 250 30 632
Cuts at: 3785 3815 4929 4959 5345 5375 5492 5522 5918
Size: 30 1114 30 386 30 117 30 396
Cuts at: 5918 5948 7181 7211 7526 7556 8864 8894 9170
Size: 30 1233 30 315 30 1308 30 276
Cuts at: 9170 9200 9458 9488 1178
Size: 30 258 30 3946
Fragments arranged by size:
3946 1440 1308 1233 1114 632 396 386
315 276 258 250 165 117 30 30
30 30 30 30 30 30 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3206 3806 4148 4411 4535 4946 6002 6297
Size: 2020 600 342 263 124 411 1056 295
Cuts at: 6297 6941 7013 7037 7040 7049 7376 8395 9178
Size: 644 72 24 3 9 327 1019 783
Cuts at: 9178 9364 9410 9413 9467 1186
Size: 186 46 3 54 3975
Fragments arranged by size:
3975 2020 1056 1019 783 644 600 411
342 327 295 263 186 124 72 54
46 24 9 3 3
BseYI C'CCAG_C
Cuts at: 2024 2285 2597 2839 2958 3038 3203 4382 4980
Size: 261 312 242 119 80 165 1179 598
Cuts at: 4980 5293 5395 5511 6117 6715 7273 7521 7751
Size: 313 102 116 606 598 558 248 230
Cuts at: 7751 7937 8162 8370 8559 8714 11459 11955 2024
Size: 186 225 208 189 155 2745 496 2325
Fragments arranged by size:
2745 2325 1179 606 598 598 558 496
313 312 261 248 242 230 225 208
189 186 165 155 119 116 102 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2580 3161 3272 4088 4271 4394 4824
Size: 573 844 581 111 816 183 123 430
Cuts at: 4824 5609 6164 6464 7346 7781 8063 8738 9110
Size: 785 555 300 882 435 282 675 372
Cuts at: 9110 9193 1163
Size: 83 4226
Fragments arranged by size:
4226 882 844 816 785 675 581 573
555 435 430 372 300 282 183 123
111 83
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2424 4056 4301 8506 9706 10467
Size: 275 186 423 1632 245 4205 1200 761
Cuts at: 10467 11495 1540
Size: 1028 2301
Fragments arranged by size:
4205 2301 1632 1200 1028 761 423 275
245 186
BsiHKAI G_wGCw'C
Cuts at: 2190 2451 3294 4595 5229 5751 6525 6789 6920
Size: 261 843 1301 634 522 774 264 131
Cuts at: 6920 8486 8906 10317 10402 11473 2190
Size: 1566 420 1411 85 1071 2973
Fragments arranged by size:
2973 1566 1411 1301 1071 843 774 634
522 420 264 261 131 85
BsiWI C'GTAC_G
Cuts at: 8197 8197
Size: 12256
BsmI GAATG_Cn'
Cuts at: 2739 3240 5382 9277 9767 9860 2739
Size: 501 2142 3895 490 93 5135
Fragments arranged by size:
5135 3895 2142 501 490 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6821 7129 8042 8407 6821
Size: 308 913 365 10670
Fragments arranged by size:
10670 913 365 308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2190 2257 2451 2475 2506 2611
Size: 399 253 147 67 194 24 31 105
Cuts at: 2611 2965 3294 3320 3389 3879 4110 4532 4595
Size: 354 329 26 69 490 231 422 63
Cuts at: 4595 4880 5025 5121 5229 5483 5751 5897 5971
Size: 285 145 96 108 254 268 146 74
Cuts at: 5971 6184 6377 6487 6525 6700 6789 6920 7434
Size: 213 193 110 38 175 89 131 514
Cuts at: 7434 7521 7872 7881 8000 8231 8486 8906 8976
Size: 87 351 9 119 231 255 420 70
Cuts at: 8976 9426 9575 10317 10402 11473 1391
Size: 450 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 514 490 450 422 420
399 354 351 329 285 268 255 254
253 231 231 213 194 193 175 149
147 146 145 131 119 110 108 105
96 89 87 85 74 70 69 67
63 38 31 26 24 9
BspEI T'CCGG_A
Cuts at: 11919 11919
Size: 12256
BspHI T'CATG_A
Cuts at: 4278 4755 7507 10145 11875 4278
Size: 477 2752 2638 1730 4659
Fragments arranged by size:
4659 2752 2638 1730 477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2520 3244 3640 3699 4101 4281 5186 6122
Size: 1676 724 396 59 402 180 905 936
Cuts at: 6122 6442 8259 8354 8552 9611 844
Size: 320 1817 95 198 1059 3489
Fragments arranged by size:
3489 1817 1676 1059 936 905 724 402
396 320 198 180 95 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2251 4147 4303 7862 10143 1394
Size: 429 428 1896 156 3559 2281 3507
Fragments arranged by size:
3559 3507 2281 1896 429 428 156
BsrDI GCAATG_nn'
Cuts at: 65 1248 2343 2678 5094 8352 10751 10925 65
Size: 1183 1095 335 2416 3258 2399 174 1396
Fragments arranged by size:
3258 2416 2399 1396 1183 1095 335 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5375 5603 6003 7836 10900 11087 1700
Size: 110 3565 228 400 1833 3064 187 2869
Fragments arranged by size:
3565 3064 2869 1833 400 228 187 110
BsrGI T'GTAC_A
Cuts at: 96 9057 96
Size: 8961 3295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12256
BssSI C'ACGA_G
Cuts at: 4289 5105 6050 7427 10316 11328 4289
Size: 816 945 1377 2889 1012 5217
Fragments arranged by size:
5217 2889 1377 1012 945 816
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4258 5733 6114 7309 8032 8514 794
Size: 3464 1475 381 1195 723 482 4536
Fragments arranged by size:
4536 3464 1475 1195 723 482 381
BstBI TT'CG_AA
Cuts at: 11060 11060
Size: 12256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12256
BstXI CCAn_nnnn'nTGG
Cuts at: 2392 2597 2639 3994 4228 5266 5371 5707 7116
Size: 205 42 1355 234 1038 105 336 1409
Cuts at: 7116 7529 2392
Size: 413 7119
Fragments arranged by size:
7119 1409 1355 1038 413 336 234 205
105 42
BstYI r'GATC_y
Cuts at: 1972 2311 2547 2754 3309 3612 3705 3781 4428
Size: 339 236 207 555 303 93 76 647
Cuts at: 4428 4494 5250 5829 6672 7072 7845 8984 9144
Size: 66 756 579 843 400 773 1139 160
Cuts at: 9144 9246 9586 9953 10338 10355 11807 11893 11905
Size: 102 340 367 385 17 1452 86 12
Cuts at: 11905 12251 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1139 843 773 756 647 579
555 400 385 367 346 340 339 303
236 207 160 102 93 86 76 66
17 12
Bsu36I CC'TnA_GG
Cuts at: 2044 2214 3321 5082 5462 6410 7441 8589 9376
Size: 170 1107 1761 380 948 1031 1148 787
Cuts at: 9376 2044
Size: 4924
Fragments arranged by size:
4924 1761 1148 1107 1031 948 787 380
170
BtgI C'CryG_G
Cuts at: 513 1065 2004 3624 3903 5635 7651 7872 8779
Size: 552 939 1620 279 1732 2016 221 907
Cuts at: 8779 8847 513
Size: 68 3922
Fragments arranged by size:
3922 2016 1732 1620 939 907 552 279
221 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5174 487
Size: 683 56 3948 7569
Fragments arranged by size:
7569 3948 683 56
BtsI GCAGTG_nn'
Cuts at: 2719 5155 6592 6624 7268 7534 8147 8209 9772
Size: 2436 1437 32 644 266 613 62 1563
Cuts at: 9772 10566 10586 2719
Size: 794 20 4389
Fragments arranged by size:
4389 2436 1563 1437 794 644 613 266
62 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6763 6798 529
Size: 35 6199 35 5987
Fragments arranged by size:
6199 5987 35 35
DraI TTT'AAA
Cuts at: 9560 9560
Size: 12256
DraIII CAC_nnn'GTG
Cuts at: 3914 3914
Size: 12256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11263 809
Size: 507 9947 1802
Fragments arranged by size:
9947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2382 2421 2999
Size: 54 1425 50 275 18 552 39 578
Cuts at: 2999 3633 3996 4059 4298 4407 4447 5373 5670
Size: 634 363 63 239 109 40 926 297
Cuts at: 5670 5912 6771 8614 8688 9703 10592 12221 8
Size: 242 859 1843 74 1015 889 1629 43
Fragments arranged by size:
1843 1629 1425 1015 926 889 859 634
578 552 363 297 275 242 239 109
74 63 54 50 43 40 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2421 4298 9703 1537
Size: 275 609 1877 5405 4090
Fragments arranged by size:
5405 4090 1877 609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3783 3819 4600 5957 6956 7014 7026 7035
Size: 2430 36 781 1357 999 58 12 9
Cuts at: 7035 7601 9387 9525 10186 1353
Size: 566 1786 138 661 3423
Fragments arranged by size:
3423 2430 1786 1357 999 781 661 566
138 58 36 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2540 3083 6824 9259 10843 11217
Size: 1064 138 951 543 3741 2435 1584 374
Cuts at: 11217 11363 387
Size: 146 1280
Fragments arranged by size:
3741 2435 1584 1280 1064 951 543 374
146 138
EcoICRI GAG'CTC
Cuts at: 5749 5749
Size: 12256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2091 2159 2255 2473 2705 2733 2868
Size: 393 459 68 96 218 232 28 135
Cuts at: 2868 2924 3097 3137 3326 3330 3431 4418 4952
Size: 56 173 40 189 4 101 987 534
Cuts at: 4952 5347 5467 5998 5999 6328 6710 6723 6863
Size: 395 120 531 1 329 382 13 140
Cuts at: 6863 6944 7183 7403 7406 7718 7848 9041 9140
Size: 81 239 220 3 312 130 1193 99
Cuts at: 9140 9173 9290 10319 10916 11703 11971 1239
Size: 33 117 1029 597 787 268 1524
Fragments arranged by size:
1524 1193 1029 987 787 597 534 531
459 395 393 382 329 312 268 239
232 220 218 189 173 140 135 130
120 117 101 99 96 81 68 56
40 33 28 13 4 3 1
EcoNI CCTnn'n_nnAGG
Cuts at: 4121 4970 7466 9152 4121
Size: 849 2496 1686 7225
Fragments arranged by size:
7225 2496 1686 849
EcoO109I rG'GnC_Cy
Cuts at: 2029 2048 2406 2472 3035 3317 3601 3856 4013
Size: 19 358 66 563 282 284 255 157
Cuts at: 4013 4255 4842 4876 4950 5021 5311 5887 5894
Size: 242 587 34 74 71 290 576 7
Cuts at: 5894 6124 6483 6484 7518 7537 7663 7877 7878
Size: 230 359 1 1034 19 126 214 1
Cuts at: 7878 8137 8524 9341 2029
Size: 259 387 817 4944
Fragments arranged by size:
4944 1034 817 587 576 563 387 359
358 290 284 282 259 255 242 230
214 157 126 74 71 66 34 19
19 7 1 1
EcoRI G'AATT_C
Cuts at: 3107 7172 9565 9943 3107
Size: 4065 2393 378 5420
Fragments arranged by size:
5420 4065 2393 378
EcoRV GAT'ATC
Cuts at: 3482 4074 3482
Size: 592 11664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3550 6997 3550
Size: 3447 8809
FspI TGC'GCA
Cuts at: 6584 10762 6584
Size: 4178 8078
HaeII r_GCGC'y
Cuts at: 1678 2369 2791 3103 4966 5845 6010 6383 6603
Size: 691 422 312 1863 879 165 373 220
Cuts at: 6603 6940 8109 8441 8710 8797 11403 1678
Size: 337 1169 332 269 87 2606 2531
Fragments arranged by size:
2606 2531 1863 1169 879 691 422 373
337 332 312 269 220 165 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2118 2150 2843 2875 3785
Size: 32 135 32 546 32 693 32 910
Cuts at: 3785 3817 3818 3850 4443 4475 5261 5293 7181
Size: 32 1 32 593 32 786 32 1888
Cuts at: 7181 7213 7352 7384 7769 7801 8020 8052 8240
Size: 32 139 32 385 32 219 32 188
Cuts at: 8240 8272 9170 9202 9458 9490 10897 10929 10971
Size: 32 898 32 256 32 1407 32 42
Cuts at: 10971 11003 1373
Size: 32 2626
Fragments arranged by size:
2626 1888 1407 910 898 786 693 593
546 385 256 219 188 139 135 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 8635 9600 9846 669
Size: 988 281 1332 5365 965 246 3079
Fragments arranged by size:
5365 3079 1332 988 965 281 246
HindIII A'AGCT_T
Cuts at: 748 2299 748
Size: 1551 10705
HpaI GTT'AAC
Cuts at: 9846 9846
Size: 12256
KasI G'GCGC_C
Cuts at: 6006 6379 6599 6006
Size: 373 220 11663
Fragments arranged by size:
11663 373 220
KpnI G_GTAC'C
Cuts at: 9581 9581
Size: 12256
MfeI C'AATT_G
Cuts at: 9855 9855
Size: 12256
MluI A'CGCG_T
Cuts at: 12132 12132
Size: 12256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3510 3783 5559 5616 8493 11370 11554 1305
Size: 2205 273 1776 57 2877 2877 184 2007
Fragments arranged by size:
2877 2877 2205 2007 1776 273 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3998 4061 5672 5914 6773
Size: 54 1425 343 2166 63 1611 242 859
Cuts at: 6773 8616 8690 10
Size: 1843 74 3576
Fragments arranged by size:
3576 2166 1843 1611 1425 859 343 242
74 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2195 2444 2522 2918 3971 4754 5387
Size: 648 1029 249 78 396 1053 783 633
Cuts at: 5387 5637 6639 7491 8663 9092 9132 9227 10214
Size: 250 1002 852 1172 429 40 95 987
Cuts at: 10214 10573 10732 518
Size: 359 159 2042
Fragments arranged by size:
2042 1172 1053 1029 1002 987 852 783
648 633 429 396 359 250 249 159
95 78 40
MspA1I CmG'CkG
Cuts at: 1606 2285 2392 2426 2821 2896 3353 3925 4052
Size: 679 107 34 395 75 457 572 127
Cuts at: 4052 5287 5733 5932 6730 6898 6964 7681 7975
Size: 1235 446 199 798 168 66 717 294
Cuts at: 7975 8116 8162 8718 9672 10349 11497 11742 1606
Size: 141 46 556 954 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 954 798 717 679 677
572 556 457 446 395 294 245 199
168 141 127 107 75 66 46 34
NaeI GCC'GGC
Cuts at: 5377 5377
Size: 12256
NarI GG'CG_CC
Cuts at: 6007 6380 6600 6007
Size: 373 220 11663
Fragments arranged by size:
11663 373 220
NcoI C'CATG_G
Cuts at: 513 1065 2004 3903 7651 7872 8779 8847 513
Size: 552 939 1899 3748 221 907 68 3922
Fragments arranged by size:
3922 3748 1899 939 907 552 221 68
NdeI CA'TA_TG
Cuts at: 387 8619 387
Size: 8232 4024
NgoMIV G'CCGG_C
Cuts at: 5375 5375
Size: 12256
NheI G'CTAG_C
Cuts at: 1052 8606 1052
Size: 7554 4702
NotI GC'GGCC_GC
Cuts at: 4298 9703 4298
Size: 5405 6851
NsiI A_TGCA'T
Cuts at: 5208 5208
Size: 12256
NspI r_CATG'y
Cuts at: 1132 1229 2024 2527 2989 3304 4046 4348 5632
Size: 97 795 503 462 315 742 302 1284
Cuts at: 5632 5695 5949 6299 7316 8272 9352 9614 11159
Size: 63 254 350 1017 956 1080 262 1545
Cuts at: 11159 1132
Size: 2229
Fragments arranged by size:
2229 1545 1284 1080 1017 956 795 742
503 462 350 315 302 262 254 97
63
PasI CC'CwG_GG
Cuts at: 1348 2234 3589 6184 7735 8233 8494 1348
Size: 886 1355 2595 1551 498 261 5110
Fragments arranged by size:
5110 2595 1551 1355 886 498 261
PciI A'CATG_T
Cuts at: 2523 5945 11155 2523
Size: 3422 5210 3624
Fragments arranged by size:
5210 3624 3422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3940 4159 4654 5910 6103 6289 8527 9019
Size: 2832 219 495 1256 193 186 2238 492
Cuts at: 9019 1108
Size: 4345
Fragments arranged by size:
4345 2832 2238 1256 495 492 219 193
186
PfoI T'CCnGG_A
Cuts at: 2314 2811 4896 5265 5922 7233 7726 8807 9150
Size: 497 2085 369 657 1311 493 1081 343
Cuts at: 9150 9249 2314
Size: 99 5321
Fragments arranged by size:
5321 2085 1311 1081 657 497 493 369
343 99
PmeI GTTT'AAAC
Cuts at: 9560 9560
Size: 12256
PmlI CAC'GTG
Cuts at: 2446 2446
Size: 12256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3203 3235 6140 6172 6814 6846 10325 10357 11838
Size: 32 2905 32 642 32 3479 32 1481
Cuts at: 11838 11870 3203
Size: 32 3589
Fragments arranged by size:
3589 3479 2905 1481 642 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2029 2406 4013 4842 5311 5887 8137 9341 2029
Size: 377 1607 829 469 576 2250 1204 4944
Fragments arranged by size:
4944 2250 1607 1204 829 576 469 377
PshAI GACnn'nnGTC
Cuts at: 1613 4364 1613
Size: 2751 9505
PsiI TTA'TAA
Cuts at: 4670 9826 4670
Size: 5156 7100
PspOMI G'GGCC_C
Cuts at: 2471 2961 3316 4876 5021 5893 6483 7517 7877
Size: 490 355 1560 145 872 590 1034 360
Cuts at: 7877 2471
Size: 6850
Fragments arranged by size:
6850 1560 1034 872 590 490 360 355
145
PstI C_TGCA'G
Cuts at: 830 2061 2577 3651 4098 4561 4603 4888 6475
Size: 1231 516 1074 447 463 42 285 1587
Cuts at: 6475 6612 6909 6936 7263 7293 7984 8124 9144
Size: 137 297 27 327 30 691 140 1020
Cuts at: 9144 9276 9608 830
Size: 132 332 3478
Fragments arranged by size:
3478 1587 1231 1074 1020 691 516 463
447 332 327 297 285 140 137 132
42 30 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12256
PvuII CAG'CTG
Cuts at: 2285 2392 2821 3353 3925 5287 5733 5932 6898
Size: 107 429 532 572 1362 446 199 966
Cuts at: 6898 7681 7975 8116 8162 8718 2285
Size: 783 294 141 46 556 5823
Fragments arranged by size:
5823 1362 966 783 572 556 532 446
429 294 199 141 107 46
RsrII CG'GwC_CG
Cuts at: 6811 6811
Size: 12256
SacI G_AGCT'C
Cuts at: 5751 5751
Size: 12256
SalI G'TCGA_C
Cuts at: 1936 3268 8633 9598 1936
Size: 1332 5365 965 4594
Fragments arranged by size:
5365 4594 1332 965
SanDI GG'GwC_CC
Cuts at: 2406 4013 4842 2406
Size: 1607 829 9820
Fragments arranged by size:
9820 1607 829
SapI GCTCTTCn'nnn_
Cuts at: 4600 4600
Size: 12256
SbfI CC_TGCA'GG
Cuts at: 7984 9608 7984
Size: 1624 10632
ScaI AGT'ACT
Cuts at: 6206 10504 12216 6206
Size: 4298 1712 6246
Fragments arranged by size:
6246 4298 1712
SexAI A'CCwGG_T
Cuts at: 4224 5313 8477 4224
Size: 1089 3164 8003
Fragments arranged by size:
8003 3164 1089
SfcI C'TryA_G
Cuts at: 826 1045 2057 2573 3647 4094 4557 4599 4884
Size: 219 1012 516 1074 447 463 42 285
Cuts at: 4884 5347 6471 6608 6905 6932 7207 7259 7289
Size: 463 1124 137 297 27 275 52 30
Cuts at: 7289 7970 7980 8120 8128 8578 9067 9140 9272
Size: 681 10 140 8 450 489 73 132
Cuts at: 9272 9604 10739 11420 11611 826
Size: 332 1135 681 191 1471
Fragments arranged by size:
1471 1135 1124 1074 1012 681 681 516
489 463 463 450 447 332 297 285
275 219 191 140 137 132 73 52
42 30 27 10 8
SfoI GGC'GCC
Cuts at: 6008 6381 6601 6008
Size: 373 220 11663
Fragments arranged by size:
11663 373 220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12256
SgrAI Cr'CCGG_yG
Cuts at: 1700 6003 1700
Size: 4303 7953
SmaI CCC'GGG
Cuts at: 1872 4527 5870 9583 1872
Size: 2655 1343 3713 4545
Fragments arranged by size:
4545 3713 2655 1343
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 4958 5683 6238 6264 7618
Size: 197 926 364 2651 725 555 26 1354
Cuts at: 7618 8801 10360 11261 11523 820
Size: 1183 1559 901 262 1553
Fragments arranged by size:
2651 1559 1553 1354 1183 926 901 725
555 364 262 197 26
SnaBI TAC'GTA
Cuts at: 493 10047 493
Size: 9554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12256
SphI G_CATG'C
Cuts at: 1132 2024 4348 8272 9614 1132
Size: 892 2324 3924 1342 3774
Fragments arranged by size:
3924 3774 2324 1342 892
SrfI GCCC'GGGC
Cuts at: 4527 4527
Size: 12256
SspI AAT'ATT
Cuts at: 5 52 10180 5
Size: 47 10128 2081
Fragments arranged by size:
10128 2081 47
StuI AGG'CCT
Cuts at: 1517 2092 2322 7703 9326 9371 9548 1517
Size: 575 230 5381 1623 45 177 4225
Fragments arranged by size:
5381 4225 1623 575 230 177 45
StyI C'CwwG_G
Cuts at: 513 1065 2004 2778 3604 3903 7651 7872 8779
Size: 552 939 774 826 299 3748 221 907
Cuts at: 8779 8847 9695 513
Size: 68 848 3074
Fragments arranged by size:
3748 3074 939 907 848 826 774 552
299 221 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 3277 3581 3908 10283 10300 10453
Size: 1255 573 1124 304 327 6375 17 153
Cuts at: 10453 10638 11083 325
Size: 185 445 1498
Fragments arranged by size:
6375 1498 1255 1124 573 445 327 304
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6204 7772 9057
Size: 274 80 33 51 1443 4227 1568 1285
Cuts at: 9057 9084 10502 12214 96
Size: 27 1418 1712 138
Fragments arranged by size:
4227 1712 1568 1443 1418 1285 274 138
80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 3379 3411 4734 4766 4929 4961 5492
Size: 32 224 32 1323 32 163 32 531
Cuts at: 5492 5524 5918 5950 7526 7558 12216 12248 3123
Size: 32 394 32 1576 32 4658 32 3131
Fragments arranged by size:
4658 3131 1576 1323 531 394 224 163
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3832 1316
Size: 2516 9740
XbaI T'CTAG_A
Cuts at: 9592 9592
Size: 12256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4835 7093 7748 4835
Size: 2258 655 9343
Fragments arranged by size:
9343 2258 655
XhoI C'TCGA_G
Cuts at: 1943 2307 6264 1943
Size: 364 3957 7935
Fragments arranged by size:
7935 3957 364
XmaI C'CCGG_G
Cuts at: 1870 4525 5868 9581 1870
Size: 2655 1343 3713 4545
Fragments arranged by size:
4545 3713 2655 1343
XmnI GAAnn'nnTTC
Cuts at: 2079 5810 10385 2079
Size: 3731 4575 3950
Fragments arranged by size:
4575 3950 3731
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10064 276
Size: 53 83 186 936 8530 2468
Fragments arranged by size:
8530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI BstZ17I FseI FspAI NruI PacI PspXI PsrI
SacII SfiI SwaI