Restriction map for
pFN21AA0705
(Circular) (Six-base) MAPSORT of: pFN21AA0705.seq Check: 1260 from: 1 to: 9028
pFN21AA0705 9028 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2777 3346 4174 2777
Size: 569 828 7631
Fragments arranged by size:
7631 828 569
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6838 278
Size: 53 83 186 936 5302 2468
Fragments arranged by size:
5302 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2958 6371 1937
Size: 1021 3413 4594
Fragments arranged by size:
4594 3413 1021
Acc65I G'GTAC_C
Cuts at: 2555 6349 2555
Size: 3794 5234
AclI AA'CG_TT
Cuts at: 1563 7155 7528 1563
Size: 5592 373 3063
Fragments arranged by size:
5592 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2120 2363 2658 2873 2910 3573 4315 4707
Size: 488 243 295 215 37 663 742 392
Cuts at: 4707 5126 7091 8475 8743 1632
Size: 419 1965 1384 268 1917
Fragments arranged by size:
1965 1917 1384 742 663 488 419 392
295 268 243 215 37
AfeI AGC'GCT
Cuts at: 5909 5909
Size: 9028
AflII C'TTAA_G
Cuts at: 820 1017 4001 820
Size: 197 2984 5847
Fragments arranged by size:
5847 2984 197
AflIII A'CryG_T
Cuts at: 1203 7927 8904 1203
Size: 6724 977 1327
Fragments arranged by size:
6724 1327 977
AgeI A'CCGG_T
Cuts at: 7859 7859
Size: 9028
AhdI GACnn_n'nnGTC
Cuts at: 3775 7757 3775
Size: 3982 5046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9028
AlwNI CAG_nnn'CTG
Cuts at: 3973 4153 4595 6771 8343 3973
Size: 180 442 2176 1572 4658
Fragments arranged by size:
4658 2176 1572 442 180
ApaI G_GGCC'C
Cuts at: 6209 6261 6209
Size: 52 8976
ApaLI G'TGCA_C
Cuts at: 7085 8241 7085
Size: 1156 7872
ApoI r'AATT_y
Cuts at: 1493 6337 6563 6715 8824 1493
Size: 4844 226 152 2109 1697
Fragments arranged by size:
4844 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3432 7582 160
Size: 872 2400 4150 1606
Fragments arranged by size:
4150 2400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9028
AvaI C'yCGr_G
Cuts at: 1870 1943 3246 4340 5891 5945 5975 6011 6109
Size: 73 1303 1094 1551 54 30 36 98
Cuts at: 6109 6136 6217 6353 1870
Size: 27 81 136 4545
Fragments arranged by size:
4545 1551 1303 1094 136 98 81 73
54 36 30 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2539 2572 2539
Size: 33 8995
BamHI G'GATC_C
Cuts at: 2998 4796 4884 6358 6725 2998
Size: 1798 88 1474 367 5301
Fragments arranged by size:
5301 1798 1474 367 88
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 2416 2555 2789 3574
Size: 325 217 627 321 308 139 234 785
Cuts at: 3574 3836 5014 5953 6007 6064 6224 6268 6308
Size: 262 1178 939 54 57 160 44 40
Cuts at: 6308 6349 7804 618
Size: 41 1455 1842
Fragments arranged by size:
1842 1455 1178 939 785 627 325 321
308 262 234 217 160 139 57 54
44 41 40
BanII G_rGCy'C
Cuts at: 1391 2235 4184 4239 4397 4566 4998 6018 6086
Size: 844 1949 55 158 169 432 1020 68
Cuts at: 6086 6093 6209 6261 6347 1391
Size: 7 116 52 86 4072
Fragments arranged by size:
4072 1949 1020 844 432 169 158 116
86 68 55 52 7
BbeI G_GCGC'C
Cuts at: 2112 5957 6011 6068 6228 6312 2112
Size: 3845 54 57 160 84 4828
Fragments arranged by size:
4828 3845 160 84 57 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2547 2781 2898 3561 3631 5623 928
Size: 971 648 234 117 663 70 1992 4333
Fragments arranged by size:
4333 1992 971 663 648 234 117 70
BbvCI CC'TCA_GC
Cuts at: 3278 3278
Size: 9028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2372 2406 7219 7253 1903
Size: 34 435 34 4813 34 3678
Fragments arranged by size:
4813 3678 435 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3759 4609 6920 8130 3759
Size: 850 2311 1210 4657
Fragments arranged by size:
4657 2311 1210 850
BclI T'GATC_A
Cuts at: 1548 2259 1548
Size: 711 8317
BfrBI ATG'CAT
Cuts at: 3254 3254
Size: 9028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2088 5999 6141
Size: 107 122 71 631 605 416 3911 142
Cuts at: 6141 7639 136
Size: 1498 1525
Fragments arranged by size:
3911 1525 1498 631 605 416 142 122
107 71
BglII A'GATC_T
Cuts at: 9023 9023
Size: 9028
BlpI GC'TnA_GC
Cuts at: 4396 6445 4396
Size: 2049 6979
Bme1580I G_kGCm'C
Cuts at: 1790 3841 4815 6209 6261 7089 8245 1790
Size: 2051 974 1394 52 828 1156 2573
Fragments arranged by size:
2573 2051 1394 1156 974 828 52
BmgBI CAC'GTC
Cuts at: 1318 2248 1318
Size: 930 8098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2935 3253 4659
Size: 416 53 83 399 24 1518 318 1406
Cuts at: 4659 6759 7717 442
Size: 2100 958 1753
Fragments arranged by size:
2100 1753 1518 1406 958 416 399 318
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2824 3621 2824
Size: 797 8231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2478 3012 3591 5631 5704 6182 7688
Size: 986 253 534 579 2040 73 478 1506
Cuts at: 7688 1239
Size: 2579
Fragments arranged by size:
2579 2040 1506 986 579 534 478 253
73
Bpu10I CC'TnA_GC
Cuts at: 3278 3974 3278
Size: 696 8332
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 5621 7153 8018 8316 2273
Size: 3348 1532 865 298 2985
Fragments arranged by size:
3348 2985 1532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 4872 7691 882
Size: 617 711 2662 2819 2219
Fragments arranged by size:
2819 2662 2219 711 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 6819 493
Size: 711 2543 3072 2702
Fragments arranged by size:
3072 2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 4299 9022 4299
Size: 4723 4305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2109 4273 5727 5954
Size: 53 83 186 936 576 2164 1454 227
Cuts at: 5954 6008 6065 6225 6309 6835 7217 7852 275
Size: 54 57 160 84 526 382 635 1451
Fragments arranged by size:
2164 1454 1451 936 635 576 526 382
227 186 160 84 83 57 54 53
BsaWI w'CCGG_w
Cuts at: 3373 4881 7461 7859 8133 8280 8691 3373
Size: 1508 2580 398 274 147 411 3710
Fragments arranged by size:
3710 2580 1508 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2298 2328 2733 2763 4166
Size: 30 165 30 895 30 405 30 1403
Cuts at: 4166 4196 1178
Size: 30 6010
Fragments arranged by size:
6010 1403 895 405 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2717 2861 4062 5006 5963 1186
Size: 1021 510 144 1201 944 957 4251
Fragments arranged by size:
4251 1201 1021 957 944 510 144
BseYI C'CCAG_C
Cuts at: 2086 2811 3063 5018 5082 5092 5118 6290 8231
Size: 725 252 1955 64 10 26 1172 1941
Cuts at: 8231 8727 2086
Size: 496 2387
Fragments arranged by size:
2387 1955 1941 1172 725 496 252 64
26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2805 2808 3695 1163
Size: 573 1069 3 887 6496
Fragments arranged by size:
6496 1069 887 573 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3698 5939 6203 6318 6478 7239
Size: 275 186 1697 2241 264 115 160 761
Cuts at: 7239 8267 1540
Size: 1028 2301
Fragments arranged by size:
2301 2241 1697 1028 761 275 264 186
160 115
BsiHKAI G_wGCw'C
Cuts at: 2518 4184 4239 4998 6093 7089 7174 8245 2518
Size: 1666 55 759 1095 996 85 1071 3301
Fragments arranged by size:
3301 1666 1095 1071 996 759 85 55
BsmI GAATG_Cn'
Cuts at: 5442 6539 6632 5442
Size: 1097 93 7838
Fragments arranged by size:
7838 1097 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3327 5734 3327
Size: 2407 6621
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2518 3841 4184 4239 4397 4566
Size: 399 445 283 1323 343 55 158 169
Cuts at: 4566 4815 4998 6018 6086 6093 6209 6261 6347
Size: 249 183 1020 68 7 116 52 86
Cuts at: 6347 7089 7174 8245 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1323 1071 1020 742 445 399 343
283 249 183 169 158 116 86 85
68 55 52 7
BspEI T'CCGG_A
Cuts at: 3373 8691 3373
Size: 5318 3710
BspHI T'CATG_A
Cuts at: 6917 8647 6917
Size: 1730 7298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2777 3346 4161 4174 4510 4946 6383 844
Size: 1933 569 815 13 336 436 1437 3489
Fragments arranged by size:
3489 1933 1437 815 569 436 336 13
BsrBI CCG'CTC
Cuts at: 1394 1823 2172 6915 1394
Size: 429 349 4743 3507
Fragments arranged by size:
4743 3507 429 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 2912 4383 4519 4678 7523 7697 65
Size: 1183 1664 1471 136 159 2845 174 1396
Fragments arranged by size:
2845 1664 1471 1396 1183 174 159 136
BsrFI r'CCGG_y
Cuts at: 1700 1810 4463 5981 6096 6121 6131 7672 7859
Size: 110 2653 1518 115 25 10 1541 187
Cuts at: 7859 1700
Size: 2869
Fragments arranged by size:
2869 2653 1541 1518 187 115 110 25
10
BsrGI T'GTAC_A
Cuts at: 96 2832 3964 96
Size: 2736 1132 5160
Fragments arranged by size:
5160 2736 1132
BssHII G'CGCG_C
Cuts at: 1925 6156 6185 6187 6233 1925
Size: 4231 29 2 46 4720
Fragments arranged by size:
4720 4231 46 29 2
BssSI C'ACGA_G
Cuts at: 7088 8100 7088
Size: 1012 8016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3718 4153 794
Size: 2924 435 5669
Fragments arranged by size:
5669 2924 435
BstBI TT'CG_AA
Cuts at: 7832 7832
Size: 9028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9028
BstXI CCAn_nnnn'nTGG
Cuts at: 3063 4723 3063
Size: 1660 7368
BstYI r'GATC_y
Cuts at: 1972 2998 4796 4884 6358 6725 7110 7127 8579
Size: 1026 1798 88 1474 367 385 17 1452
Cuts at: 8579 8665 8677 9023 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1798 1474 1452 1026 385 367 346
88 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2700 2794 2844 4986 2700
Size: 94 50 2142 6742
Fragments arranged by size:
6742 2142 94 50
BtgI C'CryG_G
Cuts at: 513 1065 2223 4064 4214 4389 5040 513
Size: 552 1158 1841 150 175 651 4501
Fragments arranged by size:
4501 1841 1158 651 552 175 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5098 487
Size: 683 56 3872 4417
Fragments arranged by size:
4417 3872 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 4630 4899 6544 7338 7358 2266
Size: 2364 269 1645 794 20 3936
Fragments arranged by size:
3936 2364 1645 794 269 20
ClaI AT'CG_AT
Cuts at: 1554 3075 1554
Size: 1521 7507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8993
DraI TTT'AAA
Cuts at: 6332 6332
Size: 9028
DraIII CAC_nnn'GTG
Cuts at: 4625 4625
Size: 9028
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3670 8035 809
Size: 507 2354 4365 1802
Fragments arranged by size:
4365 2354 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 3695 4137
Size: 54 1425 50 275 18 423 1442 442
Cuts at: 4137 4411 4900 5872 5936 5962 6200 6315 6475
Size: 274 489 972 64 26 238 115 160
Cuts at: 6475 7364 8993 8
Size: 889 1629 43
Fragments arranged by size:
1629 1442 1425 972 889 489 442 423
275 274 238 160 115 64 54 50
43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 3695 5936 6200 6315 6475 1537
Size: 275 1883 2241 264 115 160 4090
Fragments arranged by size:
4090 2241 1883 275 264 160 115
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 2703 2709 3027 3245 6958 1353
Size: 1292 58 6 318 218 3713 3423
Fragments arranged by size:
3713 3423 1292 318 218 58 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6050 7615 7989 8135 387
Size: 1064 138 4461 1565 374 146 1280
Fragments arranged by size:
4461 1565 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4182 4237 4996 6091 4182
Size: 55 759 1095 7119
Fragments arranged by size:
7119 1095 759 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2225 2363 2478 2658 2873 2910
Size: 393 488 105 138 115 180 215 37
Cuts at: 2910 3012 3573 3591 4315 4707 5126 5631 5704
Size: 102 561 18 724 392 419 505 73
Cuts at: 5704 6182 7091 7688 8475 8743 1239
Size: 478 909 597 787 268 1524
Fragments arranged by size:
1524 909 787 724 597 561 505 488
478 419 393 392 268 215 180 138
115 105 102 73 37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2138 2138
Size: 9028
EcoO109I rG'GnC_Cy
Cuts at: 2275 2452 3214 5656 6194 6257 6258 2275
Size: 177 762 2442 538 63 1 5045
Fragments arranged by size:
5045 2442 762 538 177 63 1
EcoRI G'AATT_C
Cuts at: 6337 6715 6337
Size: 378 8650
EcoRV GAT'ATC
Cuts at: 5824 5824
Size: 9028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2550 3827 4044 2550
Size: 1277 217 7534
Fragments arranged by size:
7534 1277 217
FspI TGC'GCA
Cuts at: 7534 7534
Size: 9028
HaeII r_GCGC'y
Cuts at: 1678 2112 5890 5911 5957 6011 6068 6228 6312
Size: 434 3778 21 46 54 57 160 84
Cuts at: 6312 8175 1678
Size: 1863 2531
Fragments arranged by size:
3778 2531 1863 434 160 84 57 54
46 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3306 3338 3761 3793 4027
Size: 32 135 32 1734 32 423 32 234
Cuts at: 4027 4059 4781 4813 4869 4901 5526 5558 7669
Size: 32 722 32 56 32 625 32 2111
Cuts at: 7669 7701 7743 7775 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2111 1734 722 625 423 234 135
56 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 3511 4525 6372 6618 669
Size: 988 281 1168 405 1014 1847 246 3079
Fragments arranged by size:
3079 1847 1168 1014 988 405 281 246
HindIII A'AGCT_T
Cuts at: 748 2014 748
Size: 1266 7762
HpaI GTT'AAC
Cuts at: 6618 6618
Size: 9028
KasI G'GCGC_C
Cuts at: 2108 5953 6007 6064 6224 6308 2108
Size: 3845 54 57 160 84 4828
Fragments arranged by size:
4828 3845 160 84 57 54
KpnI G_GTAC'C
Cuts at: 2559 6353 2559
Size: 3794 5234
MfeI C'AATT_G
Cuts at: 6627 6627
Size: 9028
MluI A'CGCG_T
Cuts at: 8904 8904
Size: 9028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 5236 5346 5808 8142 8326 1305
Size: 864 3067 110 462 2334 184 2007
Fragments arranged by size:
3067 2334 2007 864 462 184 110
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4902 10
Size: 54 1425 343 3070 4136
Fragments arranged by size:
4136 3070 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 3459 3465 3633 4388 4479 4916
Size: 648 889 1404 6 168 755 91 437
Cuts at: 4916 6986 7345 7504 518
Size: 2070 359 159 2042
Fragments arranged by size:
2070 2042 1404 889 755 648 437 359
168 159 91 6
MspA1I CmG'CkG
Cuts at: 1606 2086 3883 5146 5248 5696 6153 6444 7121
Size: 480 1797 1263 102 448 457 291 677
Cuts at: 7121 8269 8514 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1797 1263 1148 677 480 457 448
291 245 102
NaeI GCC'GGC
Cuts at: 4465 5983 6098 6123 6133 4465
Size: 1518 115 25 10 7360
Fragments arranged by size:
7360 1518 115 25 10
NarI GG'CG_CC
Cuts at: 2109 5954 6008 6065 6225 6309 2109
Size: 3845 54 57 160 84 4828
Fragments arranged by size:
4828 3845 160 84 57 54
NcoI C'CATG_G
Cuts at: 513 1065 4064 4389 5040 513
Size: 552 2999 325 651 4501
Fragments arranged by size:
4501 2999 651 552 325
NdeI CA'TA_TG
Cuts at: 387 3058 4231 387
Size: 2671 1173 5184
Fragments arranged by size:
5184 2671 1173
NgoMIV G'CCGG_C
Cuts at: 4463 5981 6096 6121 6131 4463
Size: 1518 115 25 10 7360
Fragments arranged by size:
7360 1518 115 25 10
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9028
NotI GC'GGCC_GC
Cuts at: 6475 6475
Size: 9028
NsiI A_TGCA'T
Cuts at: 3256 3256
Size: 9028
NspI r_CATG'y
Cuts at: 1132 1229 3181 4921 5585 6386 7931 1132
Size: 97 1952 1740 664 801 1545 2229
Fragments arranged by size:
2229 1952 1740 1545 801 664 97
PasI CC'CwG_GG
Cuts at: 1348 3841 1348
Size: 2493 6535
PciI A'CATG_T
Cuts at: 7927 7927
Size: 9028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3728 1108
Size: 2620 6408
PfoI T'CCnGG_A
Cuts at: 2617 3144 2617
Size: 527 8501
PmeI GTTT'AAAC
Cuts at: 6332 6332
Size: 9028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7097 7129 8610 8642 7097
Size: 32 1481 32 7483
Fragments arranged by size:
7483 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 2452 5656 6194 2275
Size: 177 3204 538 5109
Fragments arranged by size:
5109 3204 538 177
PshAI GACnn'nnGTC
Cuts at: 1613 2328 1613
Size: 715 8313
PsiI TTA'TAA
Cuts at: 4284 6598 4284
Size: 2314 6714
PspOMI G'GGCC_C
Cuts at: 6205 6257 6205
Size: 52 8976
PspXI vC'TCGA_Gb
Cuts at: 5945 5945
Size: 9028
PstI C_TGCA'G
Cuts at: 830 3213 3357 3388 4713 4852 6380 830
Size: 2383 144 31 1325 139 1528 3478
Fragments arranged by size:
3478 2383 1528 1325 144 139 31
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9028
PvuII CAG'CTG
Cuts at: 2086 5146 2086
Size: 3060 5968
SacI G_AGCT'C
Cuts at: 4184 4239 4998 6093 4184
Size: 55 759 1095 7119
Fragments arranged by size:
7119 1095 759 55
SalI G'TCGA_C
Cuts at: 1936 6370 1936
Size: 4434 4594
SanDI GG'GwC_CC
Cuts at: 6194 6194
Size: 9028
SbfI CC_TGCA'GG
Cuts at: 3213 6380 3213
Size: 3167 5861
ScaI AGT'ACT
Cuts at: 7276 8988 7276
Size: 1712 7316
SexAI A'CCwGG_T
Cuts at: 3513 3513
Size: 9028
SfcI C'TryA_G
Cuts at: 826 1045 3164 3209 3353 3384 3873 4709 4848
Size: 219 2119 45 144 31 489 836 139
Cuts at: 4848 5233 5302 5326 5371 6376 7511 8192 8383
Size: 385 69 24 45 1005 1135 681 191
Cuts at: 8383 826
Size: 1471
Fragments arranged by size:
2119 1471 1135 1005 836 681 489 385
219 191 144 139 69 45 45 31
24
SfoI GGC'GCC
Cuts at: 2110 5955 6009 6066 6226 6310 2110
Size: 3845 54 57 160 84 4828
Fragments arranged by size:
4828 3845 160 84 57 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9028
SgrAI Cr'CCGG_yG
Cuts at: 1700 6121 1700
Size: 4421 4607
SmaI CCC'GGG
Cuts at: 1872 3248 6013 6111 6138 6219 6355 1872
Size: 1376 2765 98 27 81 136 4545
Fragments arranged by size:
4545 2765 1376 136 98 81 27
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2288 4001 5636 5945 7132 8033
Size: 197 926 345 1713 1635 309 1187 901
Cuts at: 8033 8295 820
Size: 262 1553
Fragments arranged by size:
1713 1635 1553 1187 926 901 345 309
262 197
SnaBI TAC'GTA
Cuts at: 493 6819 493
Size: 6326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9028
SphI G_CATG'C
Cuts at: 1132 6386 1132
Size: 5254 3774
SrfI GCCC'GGGC
Cuts at: 6013 6138 6013
Size: 125 8903
SspI AAT'ATT
Cuts at: 5 52 6952 5
Size: 47 6900 2081
Fragments arranged by size:
6900 2081 47
StuI AGG'CCT
Cuts at: 1517 2722 4205 4786 1517
Size: 1205 1483 581 5759
Fragments arranged by size:
5759 1483 1205 581
StyI C'CwwG_G
Cuts at: 513 1065 3448 4064 4389 5040 5295 6467 513
Size: 552 2383 616 325 651 255 1172 3074
Fragments arranged by size:
3074 2383 1172 651 616 552 325 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 4107 4437 4619 5032 7055 7072
Size: 1255 2235 292 330 182 413 2023 17
Cuts at: 7072 7225 7410 7855 325
Size: 153 185 445 1498
Fragments arranged by size:
2235 2023 1498 1255 445 413 330 292
185 182 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 3651 3964
Size: 274 80 33 51 1443 855 819 313
Cuts at: 3964 7274 8986 96
Size: 3310 1712 138
Fragments arranged by size:
3310 1712 1443 855 819 313 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3744 3776 3870 3902 4166 4198 5607 5639 8988
Size: 32 94 32 264 32 1409 32 3349
Cuts at: 8988 9020 3744
Size: 32 3752
Fragments arranged by size:
3752 3349 1409 264 94 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9028
XbaI T'CTAG_A
Cuts at: 6364 6364
Size: 9028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3191 5814 3191
Size: 2623 6405
XhoI C'TCGA_G
Cuts at: 1943 5945 1943
Size: 4002 5026
XmaI C'CCGG_G
Cuts at: 1870 3246 6011 6109 6136 6217 6353 1870
Size: 1376 2765 98 27 81 136 4545
Fragments arranged by size:
4545 2765 1376 136 98 81 27
XmnI GAAnn'nnTTC
Cuts at: 7157 7157
Size: 9028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6836 276
Size: 53 83 186 936 5302 2468
Fragments arranged by size:
5302 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BstZ17I FseI FspAI NruI
PacI PmlI PsrI RsrII SacII SapI SfiI SwaI