Restriction map for
pFN21AA0698
(Circular) (Six-base) MAPSORT of: pFN21AA0698.seq Check: 4271 from: 1 to: 7378
pFN21AA0698 7378 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3147 3147
Size: 7378
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5188 278
Size: 53 83 186 936 3652 2468
Fragments arranged by size:
3652 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4721 1937
Size: 2784 4594
Acc65I G'GTAC_C
Cuts at: 2225 4596 4699 2225
Size: 2371 103 4904
Fragments arranged by size:
4904 2371 103
AclI AA'CG_TT
Cuts at: 1563 5505 5878 1563
Size: 3942 373 3063
Fragments arranged by size:
3942 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3188 5441 6825 7093 1632
Size: 1556 2253 1384 268 1917
Fragments arranged by size:
2253 1917 1556 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 4408 820
Size: 197 3391 3790
Fragments arranged by size:
3790 3391 197
AflIII A'CryG_T
Cuts at: 1203 2052 2068 3108 3876 6277 7254 1203
Size: 849 16 1040 768 2401 977 1327
Fragments arranged by size:
2401 1327 1040 977 849 768 16
AgeI A'CCGG_T
Cuts at: 6209 6209
Size: 7378
AhdI GACnn_n'nnGTC
Cuts at: 6107 6107
Size: 7378
AleI CACnn'nnGTG
Cuts at: 1166 2165 2192 1166
Size: 999 27 6352
Fragments arranged by size:
6352 999 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3212 4109 1184
Size: 2028 897 4453
Fragments arranged by size:
4453 2028 897
AlwNI CAG_nnn'CTG
Cuts at: 2140 3161 5121 6693 2140
Size: 1021 1960 1572 2825
Fragments arranged by size:
2825 1960 1572 1021
ApaI G_GGCC'C
Cuts at: 2870 3819 3900 2870
Size: 949 81 6348
Fragments arranged by size:
6348 949 81
ApaLI G'TGCA_C
Cuts at: 2058 5435 6591 2058
Size: 3377 1156 2845
Fragments arranged by size:
3377 2845 1156
ApoI r'AATT_y
Cuts at: 1493 2035 2416 2851 2933 3962 4687 4913 5065
Size: 542 381 435 82 1029 725 226 152
Cuts at: 5065 7174 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1029 725 542 435 381 226
152 82
AseI AT'TA_AT
Cuts at: 160 1032 3072 5932 160
Size: 872 2040 2860 1606
Fragments arranged by size:
2860 2040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7378
AvaI C'yCGr_G
Cuts at: 1870 1943 4703 1870
Size: 73 2760 4545
Fragments arranged by size:
4545 2760 73
BamHI G'GATC_C
Cuts at: 2794 3588 3862 4042 4708 5075 2794
Size: 794 274 180 666 367 5097
Fragments arranged by size:
5097 794 666 367 274 180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2209 2225 2891 3135 4596
Size: 325 217 627 422 16 666 244 1461
Cuts at: 4596 4699 6154 618
Size: 103 1455 1842
Fragments arranged by size:
1842 1461 1455 666 627 422 325 244
217 103 16
BanII G_rGCy'C
Cuts at: 1391 2465 2870 3531 3819 3900 3933 4697 1391
Size: 1074 405 661 288 81 33 764 4072
Fragments arranged by size:
4072 1074 764 661 405 288 81 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2608 928
Size: 971 709 5698
Fragments arranged by size:
5698 971 709
BbvCI CC'TCA_GC
Cuts at: 2589 2589
Size: 7378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5569 5603 1903
Size: 34 50 34 3548 34 3678
Fragments arranged by size:
3678 3548 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5270 6480 5270
Size: 1210 6168
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7378
BfrBI ATG'CAT
Cuts at: 2008 2539 3064 4096 2008
Size: 531 525 1032 5290
Fragments arranged by size:
5290 1032 531 525
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2865 3306 3918
Size: 107 122 71 631 605 1193 441 612
Cuts at: 3918 5989 136
Size: 2071 1525
Fragments arranged by size:
2071 1525 1193 631 612 605 441 122
107 71
BglII A'GATC_T
Cuts at: 7373 7373
Size: 7378
BlpI GC'TnA_GC
Cuts at: 3759 4795 3759
Size: 1036 6342
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2214 2483 2870 3155 3819 3900 4455
Size: 272 152 269 387 285 664 81 555
Cuts at: 4455 5439 6595 1790
Size: 984 1156 2573
Fragments arranged by size:
2573 1156 984 664 555 387 285 272
269 152 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7378
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2418 3346 5109
Size: 416 53 83 399 24 1001 928 1763
Cuts at: 5109 6067 442
Size: 958 1753
Fragments arranged by size:
1763 1753 1001 958 928 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 3947 6038 1239
Size: 1210 1498 2091 2579
Fragments arranged by size:
2579 2091 1498 1210
Bpu10I CC'TnA_GC
Cuts at: 2043 2589 2790 3512 2043
Size: 546 201 722 5909
Fragments arranged by size:
5909 722 546 201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5503 6368 6666 5503
Size: 865 298 6215
Fragments arranged by size:
6215 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 6041 882
Size: 617 2868 1674 2219
Fragments arranged by size:
2868 2219 1674 617
BsaAI yAC'GTr
Cuts at: 493 1204 5169 493
Size: 711 3965 2702
Fragments arranged by size:
3965 2702 711
BsaBI GATnn'nnATC
Cuts at: 2556 4194 7372 2556
Size: 1638 3178 2562
Fragments arranged by size:
3178 2562 1638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5185 5567 6202 275
Size: 53 83 186 936 3652 382 635 1451
Fragments arranged by size:
3652 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3450 5811 6209 6483 6630 7041 3450
Size: 2361 398 274 147 411 3787
Fragments arranged by size:
3787 2361 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 4143 4173 1178
Size: 30 165 30 1212 30 1498 30 4383
Fragments arranged by size:
4383 1498 1212 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7378
BseYI C'CCAG_C
Cuts at: 2634 3456 4489 6581 7077 2634
Size: 822 1033 2092 496 2935
Fragments arranged by size:
2935 2092 1033 822 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2902 2922 3218 3243 1163
Size: 573 1166 20 296 25 5298
Fragments arranged by size:
5298 1166 573 296 25 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4828 5589 6617 1540
Size: 275 186 2827 761 1028 2301
Fragments arranged by size:
2827 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2062 2465 5439 5524 6595 2062
Size: 403 2974 85 1071 2845
Fragments arranged by size:
2974 2845 1071 403 85
BsmI GAATG_Cn'
Cuts at: 4889 4982 4889
Size: 93 7285
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2214 2465 2483 2870 3155 3531
Size: 399 272 152 251 18 387 285 376
Cuts at: 3531 3819 3900 3933 4455 4697 5439 5524 6595
Size: 288 81 33 522 242 742 85 1071
Cuts at: 6595 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 522 399 387 376 288
285 272 251 242 152 85 81 33
18
BspEI T'CCGG_A
Cuts at: 7041 7041
Size: 7378
BspHI T'CATG_A
Cuts at: 5267 6997 5267
Size: 1730 5648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2331 3102 3147 4733 844
Size: 1487 771 45 1586 3489
Fragments arranged by size:
3489 1586 1487 771 45
BsrBI CCG'CTC
Cuts at: 1394 1823 5265 1394
Size: 429 3442 3507
Fragments arranged by size:
3507 3442 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2104 2989 4483 5873 6047 65
Size: 1183 856 885 1494 1390 174 1396
Fragments arranged by size:
1494 1396 1390 1183 885 856 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2777 6022 6209 1700
Size: 110 967 3245 187 2869
Fragments arranged by size:
3245 2869 967 187 110
BsrGI T'GTAC_A
Cuts at: 96 2694 96
Size: 2598 4780
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7378
BssSI C'ACGA_G
Cuts at: 5438 6450 5438
Size: 1012 6366
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7378
BstBI TT'CG_AA
Cuts at: 6182 6182
Size: 7378
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7378
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 3359 2611
Size: 748 6630
BstYI r'GATC_y
Cuts at: 1972 2794 2833 3588 3843 3862 4042 4195 4270
Size: 822 39 755 255 19 180 153 75
Cuts at: 4270 4500 4708 5075 5460 5477 6929 7015 7027
Size: 230 208 367 385 17 1452 86 12
Cuts at: 7027 7373 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 822 755 385 367 346 255
230 208 180 153 86 75 39 19
17 12
Bsu36I CC'TnA_GG
Cuts at: 4127 4127
Size: 7378
BtgI C'CryG_G
Cuts at: 513 1065 2128 2149 2304 2656 3934 4030 4179
Size: 552 1063 21 155 352 1278 96 149
Cuts at: 4179 513
Size: 3712
Fragments arranged by size:
3712 1278 1063 552 352 155 149 96
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6639
Fragments arranged by size:
6639 683 56
BtsI GCAGTG_nn'
Cuts at: 2784 3864 4336 4455 4894 5688 5708 2784
Size: 1080 472 119 439 794 20 4454
Fragments arranged by size:
4454 1080 794 472 439 119 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7343
DraI TTT'AAA
Cuts at: 4682 4682
Size: 7378
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6385 809
Size: 507 5069 1802
Fragments arranged by size:
5069 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3255 3361 3596
Size: 54 1425 50 275 18 1425 106 235
Cuts at: 3596 3746 4176 4308 4825 5714 7343 8
Size: 150 430 132 517 889 1629 43
Fragments arranged by size:
1629 1425 1425 889 517 430 275 235
150 132 106 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4825 1537
Size: 275 3013 4090
Fragments arranged by size:
4090 3013 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2453 2951 4385 5308 1353
Size: 1100 498 1434 923 3423
Fragments arranged by size:
3423 1434 1100 923 498
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5965 6339 6485 387
Size: 1064 138 4376 374 146 1280
Fragments arranged by size:
4376 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2463 2463
Size: 7378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 3188 3947 5441 6038 6825 7093
Size: 393 817 739 759 1494 597 787 268
Cuts at: 7093 1239
Size: 1524
Fragments arranged by size:
1524 1494 817 787 759 739 597 393
268
EcoO109I rG'GnC_Cy
Cuts at: 3896 3897 4035 3896
Size: 1 138 7239
Fragments arranged by size:
7239 138 1
EcoRI G'AATT_C
Cuts at: 2933 4687 5065 2933
Size: 1754 378 5246
Fragments arranged by size:
5246 1754 378
EcoRV GAT'ATC
Cuts at: 3664 3664
Size: 7378
FspI TGC'GCA
Cuts at: 5884 5884
Size: 7378
HaeII r_GCGC'y
Cuts at: 1678 6525 1678
Size: 4847 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2693 2725 6019 6051 6093
Size: 32 135 32 1121 32 3294 32 42
Cuts at: 6093 6125 1373
Size: 32 2626
Fragments arranged by size:
3294 2626 1121 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4722 4968 669
Size: 988 281 2784 246 3079
Fragments arranged by size:
3079 2784 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4658 748
Size: 3910 3468
HpaI GTT'AAC
Cuts at: 4968 4968
Size: 7378
KpnI G_GTAC'C
Cuts at: 2229 4600 4703 2229
Size: 2371 103 4904
Fragments arranged by size:
4904 2371 103
MfeI C'AATT_G
Cuts at: 4977 4977
Size: 7378
MluI A'CGCG_T
Cuts at: 7254 7254
Size: 7378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 2988 3040 6492 6676 1305
Size: 1632 51 52 3452 184 2007
Fragments arranged by size:
3452 2007 1632 184 52 51
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3257 3363 3748 4178 4310
Size: 54 1425 343 1425 106 385 430 132
Cuts at: 4310 10
Size: 3078
Fragments arranged by size:
3078 1425 1425 430 385 343 132 106
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2163 2165 2192 2609 2813 3717 4215
Size: 648 997 2 27 417 204 904 498
Cuts at: 4215 4341 4359 5336 5695 5854 518
Size: 126 18 977 359 159 2042
Fragments arranged by size:
2042 997 977 904 648 498 417 359
204 159 126 27 18 2
MspA1I CmG'CkG
Cuts at: 1606 2757 2872 2999 4794 5471 6619 6864 1606
Size: 1151 115 127 1795 677 1148 245 2120
Fragments arranged by size:
2120 1795 1151 1148 677 245 127 115
NcoI C'CATG_G
Cuts at: 513 1065 2128 2304 2656 3934 4030 4179 513
Size: 552 1063 176 352 1278 96 149 3712
Fragments arranged by size:
3712 1278 1063 552 352 176 149 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7378
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7378
NotI GC'GGCC_GC
Cuts at: 4825 4825
Size: 7378
NsiI A_TGCA'T
Cuts at: 2010 2541 3066 4098 2010
Size: 531 525 1032 5290
Fragments arranged by size:
5290 1032 531 525
NspI r_CATG'y
Cuts at: 1132 1229 2012 2056 2275 2650 3068 3112 3716
Size: 97 783 44 219 375 418 44 604
Cuts at: 3716 3880 4100 4174 4498 4736 6281 1132
Size: 164 220 74 324 238 1545 2229
Fragments arranged by size:
2229 1545 783 604 418 375 324 238
220 219 164 97 74 44 44
PasI CC'CwG_GG
Cuts at: 1348 2214 3812 4280 4319 1348
Size: 866 1598 468 39 4407
Fragments arranged by size:
4407 1598 866 468 39
PciI A'CATG_T
Cuts at: 2052 3108 3876 6277 2052
Size: 1056 768 2401 3153
Fragments arranged by size:
3153 2401 1056 768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3605 4192 1108
Size: 2497 587 4294
Fragments arranged by size:
4294 2497 587
PfoI T'CCnGG_A
Cuts at: 3839 3924 4641 3839
Size: 85 717 6576
Fragments arranged by size:
6576 717 85
PmeI GTTT'AAAC
Cuts at: 4682 4682
Size: 7378
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5447 5479 6960 6992 5447
Size: 32 1481 32 5833
Fragments arranged by size:
5833 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4035 4035
Size: 7378
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7378
PsiI TTA'TAA
Cuts at: 4948 4948
Size: 7378
PspOMI G'GGCC_C
Cuts at: 2866 3815 3896 2866
Size: 949 81 6348
Fragments arranged by size:
6348 949 81
PstI C_TGCA'G
Cuts at: 830 2833 3859 4730 830
Size: 2003 1026 871 3478
Fragments arranged by size:
3478 2003 1026 871
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7378
PvuII CAG'CTG
Cuts at: 2999 2999
Size: 7378
SacI G_AGCT'C
Cuts at: 2465 2465
Size: 7378
SalI G'TCGA_C
Cuts at: 1936 4720 1936
Size: 2784 4594
SanDI GG'GwC_CC
Cuts at: 4035 4035
Size: 7378
SapI GCTCTTCn'nnn_
Cuts at: 2453 2453
Size: 7378
SbfI CC_TGCA'GG
Cuts at: 4730 4730
Size: 7378
ScaI AGT'ACT
Cuts at: 2348 2407 5626 7338 2348
Size: 59 3219 1712 2388
Fragments arranged by size:
3219 2388 1712 59
SexAI A'CCwGG_T
Cuts at: 2221 2228 2221
Size: 7 7371
SfcI C'TryA_G
Cuts at: 826 1045 2829 3855 4061 4726 5861 6542 6733
Size: 219 1784 1026 206 665 1135 681 191
Cuts at: 6733 826
Size: 1471
Fragments arranged by size:
1784 1471 1135 1026 681 665 219 206
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2865 2865
Size: 7378
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7378
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7378
SmaI CCC'GGG
Cuts at: 1872 4705 1872
Size: 2833 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4408 5482 6383 6645 820
Size: 197 926 2465 1074 901 262 1553
Fragments arranged by size:
2465 1553 1074 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5169 493
Size: 4676 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7378
SphI G_CATG'C
Cuts at: 1132 2012 2275 2650 3068 3716 4100 4498 4736
Size: 880 263 375 418 648 384 398 238
Cuts at: 4736 1132
Size: 3774
Fragments arranged by size:
3774 880 648 418 398 384 375 263
238
SspI AAT'ATT
Cuts at: 5 52 5302 5
Size: 47 5250 2081
Fragments arranged by size:
5250 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7378
StyI C'CwwG_G
Cuts at: 513 1065 2128 2304 2656 2895 3277 3599 3900
Size: 552 1063 176 352 239 382 322 301
Cuts at: 3900 3934 4030 4147 4179 4817 513
Size: 34 96 117 32 638 3074
Fragments arranged by size:
3074 1063 638 552 382 352 322 301
239 176 117 96 34 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2585 2888 5405 5422 5575 5760 6205
Size: 1255 1005 303 2517 17 153 185 445
Cuts at: 6205 325
Size: 1498
Fragments arranged by size:
2517 1498 1255 1005 445 303 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2346 2405 2694
Size: 274 80 33 51 1443 369 59 289
Cuts at: 2694 2817 5624 7336 96
Size: 123 2807 1712 138
Fragments arranged by size:
2807 1712 1443 369 289 274 138 123
80 59 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4143 4175 7338 7370 4143
Size: 32 3163 32 4151
Fragments arranged by size:
4151 3163 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4355 1316
Size: 3039 4339
XbaI T'CTAG_A
Cuts at: 4714 4714
Size: 7378
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4361 4361
Size: 7378
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7378
XmaI C'CCGG_G
Cuts at: 1870 4703 1870
Size: 2833 4545
XmnI GAAnn'nnTTC
Cuts at: 2508 2937 5507 2508
Size: 429 2570 4379
Fragments arranged by size:
4379 2570 429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5186 276
Size: 53 83 186 936 3652 2468
Fragments arranged by size:
3652 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI
RsrII SacII SfoI SrfI SwaI