Restriction map for
pFN21AA0687
(Circular) (Six-base) MAPSORT of: pFN21AA0687.seq Check: 2449 from: 1 to: 7816
pFN21AA0687 7816 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5626 278
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2131 3053 4347 5159 1937
Size: 194 922 1294 812 4594
Fragments arranged by size:
4594 1294 922 812 194
Acc65I G'GTAC_C
Cuts at: 5137 5137
Size: 7816
AclI AA'CG_TT
Cuts at: 1563 3100 5943 6316 1563
Size: 1537 2843 373 3063
Fragments arranged by size:
3063 2843 1537 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2783 3460 3522 3567 5879 7263 7531 1632
Size: 1151 677 62 45 2312 1384 268 1917
Fragments arranged by size:
2312 1917 1384 1151 677 268 62 45
AflII C'TTAA_G
Cuts at: 820 1017 2909 820
Size: 197 1892 5727
Fragments arranged by size:
5727 1892 197
AflIII A'CryG_T
Cuts at: 1203 2134 4342 4708 6715 7692 1203
Size: 931 2208 366 2007 977 1327
Fragments arranged by size:
2208 2007 1327 977 931 366
AgeI A'CCGG_T
Cuts at: 6647 6647
Size: 7816
AhdI GACnn_n'nnGTC
Cuts at: 6545 6545
Size: 7816
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7816
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4964 4996 4964
Size: 32 7784
AlwNI CAG_nnn'CTG
Cuts at: 3523 5559 7131 3523
Size: 2036 1572 4208
Fragments arranged by size:
4208 2036 1572
ApaLI G'TGCA_C
Cuts at: 5873 7029 5873
Size: 1156 6660
ApoI r'AATT_y
Cuts at: 1493 3781 4186 4478 4544 5009 5125 5351 5503
Size: 2288 405 292 66 465 116 226 152
Cuts at: 5503 7612 1493
Size: 2109 1697
Fragments arranged by size:
2288 2109 1697 465 405 292 226 152
116 66
AseI AT'TA_AT
Cuts at: 160 1032 6370 160
Size: 872 5338 1606
Fragments arranged by size:
5338 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
AvaI C'yCGr_G
Cuts at: 1870 1943 3185 3434 5141 1870
Size: 73 1242 249 1707 4545
Fragments arranged by size:
4545 1707 1242 249 73
BamHI G'GATC_C
Cuts at: 2062 3425 4640 5146 5513 2062
Size: 1363 1215 506 367 4365
Fragments arranged by size:
4365 1363 1215 506 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2808 3379 4525 5137
Size: 325 217 627 318 703 571 1146 612
Cuts at: 5137 6592 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1146 703 627 612 571 325
318 217
BanII G_rGCy'C
Cuts at: 1391 2285 3192 3348 4999 5135 1391
Size: 894 907 156 1651 136 4072
Fragments arranged by size:
4072 1651 907 894 156 136
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2391 4273 928
Size: 971 492 1882 4471
Fragments arranged by size:
4471 1882 971 492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2003 2037 3862 3896 6007 6041 1903
Size: 34 66 34 1825 34 2111 34 3678
Fragments arranged by size:
3678 2111 1825 66 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5708 6918 5708
Size: 1210 6606
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7816
BfrBI ATG'CAT
Cuts at: 2713 2713
Size: 7816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6427 136
Size: 107 122 71 631 605 4755 1525
Fragments arranged by size:
4755 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7811 7811
Size: 7816
BlpI GC'TnA_GC
Cuts at: 2532 5233 2532
Size: 2701 5115
Bme1580I G_kGCm'C
Cuts at: 1790 3384 3875 4528 5877 7033 1790
Size: 1594 491 653 1349 1156 2573
Fragments arranged by size:
2573 1594 1349 1156 653 491
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7816
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5547 6505 442
Size: 416 53 83 399 24 4130 958 1753
Fragments arranged by size:
4130 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7816
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 7816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2271 2389 3899 6476 1239
Size: 989 43 118 1510 2577 2579
Fragments arranged by size:
2579 2577 1510 989 118 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3560 4313 5941 6806 7104 3560
Size: 753 1628 865 298 4272
Fragments arranged by size:
4272 1628 865 753 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3444 3456 4027 6479 882
Size: 617 1945 12 571 2452 2219
Fragments arranged by size:
2452 2219 1945 617 571 12
BsaAI yAC'GTr
Cuts at: 493 1204 4343 5607 493
Size: 711 3139 1264 2702
Fragments arranged by size:
3139 2702 1264 711
BsaBI GATnn'nnATC
Cuts at: 3490 7810 3490
Size: 4320 3496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5623 6005 6640 275
Size: 53 83 186 936 4090 382 635 1451
Fragments arranged by size:
4090 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6249 6647 6921 7068 7479 6249
Size: 398 274 147 411 6586
Fragments arranged by size:
6586 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 4560 4590 1178
Size: 30 165 30 633 30 2494 30 4404
Fragments arranged by size:
4404 2494 633 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2742 3008 3394 3658 3695 3716 3925
Size: 855 701 266 386 264 37 21 209
Cuts at: 3925 1186
Size: 5077
Fragments arranged by size:
5077 855 701 386 266 264 209 37
21
BseYI C'CCAG_C
Cuts at: 2069 2333 3429 4923 5112 7019 7515 2069
Size: 264 1096 1494 189 1907 496 2370
Fragments arranged by size:
2370 1907 1494 1096 496 264 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7243
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5266 6027 7055 1540
Size: 275 186 3265 761 1028 2301
Fragments arranged by size:
3265 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2533 2695 2728 5877 5962 7033 2533
Size: 162 33 3149 85 1071 3316
Fragments arranged by size:
3316 3149 1071 162 85 33
BsmI GAATG_Cn'
Cuts at: 2500 5327 5420 2500
Size: 2827 93 4896
Fragments arranged by size:
4896 2827 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2285 2533 2695 2728 3192 3348 3384
Size: 399 495 248 162 33 464 156 36
Cuts at: 3384 3875 4528 4999 5135 5877 5962 7033 1391
Size: 491 653 471 136 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 653 495 491 471 464
399 248 162 156 136 85 36 33
BspEI T'CCGG_A
Cuts at: 7479 7479
Size: 7816
BspHI T'CATG_A
Cuts at: 5082 5705 7435 5082
Size: 623 1730 5463
Fragments arranged by size:
5463 1730 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5171 844
Size: 4327 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5703 1394
Size: 429 3880 3507
Fragments arranged by size:
3880 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2247 3796 5032 6311 6485 65
Size: 1183 999 1549 1236 1279 174 1396
Fragments arranged by size:
1549 1396 1279 1236 1183 999 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3847 6460 6647 1700
Size: 110 2037 2613 187 2869
Fragments arranged by size:
2869 2613 2037 187 110
BsrGI T'GTAC_A
Cuts at: 96 4444 96
Size: 4348 3468
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7816
BssSI C'ACGA_G
Cuts at: 5876 6888 5876
Size: 1012 6804
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2690 794
Size: 1896 5920
BstBI TT'CG_AA
Cuts at: 6620 6620
Size: 7816
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7816
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 2680
Size: 7816
BstYI r'GATC_y
Cuts at: 1972 2062 3425 3595 4054 4597 4640 4944 5146
Size: 90 1363 170 459 543 43 304 202
Cuts at: 5146 5513 5898 5915 7367 7453 7465 7811 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1363 543 459 385 367 346
304 202 170 90 86 43 17 12
Bsu36I CC'TnA_GG
Cuts at: 2417 2595 3406 3745 5089 2417
Size: 178 811 339 1344 5144
Fragments arranged by size:
5144 1344 811 339 178
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6325
Fragments arranged by size:
6325 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2587 487
Size: 683 56 1361 5716
Fragments arranged by size:
5716 1361 683 56
BtsI GCAGTG_nn'
Cuts at: 3281 3298 3400 3540 3633 5332 6126 6146 3281
Size: 17 102 140 93 1699 794 20 4951
Fragments arranged by size:
4951 1699 794 140 102 93 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7816
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7781
DraI TTT'AAA
Cuts at: 5120 5120
Size: 7816
DraIII CAC_nnn'GTG
Cuts at: 4112 4112
Size: 7816
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6823 809
Size: 507 5507 1802
Fragments arranged by size:
5507 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3609 3641 3849
Size: 54 1425 50 275 18 1779 32 208
Cuts at: 3849 4234 5263 6152 7781 8
Size: 385 1029 889 1629 43
Fragments arranged by size:
1779 1629 1425 1029 889 385 275 208
54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 5263 1537
Size: 275 3451 4090
Fragments arranged by size:
4090 3451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2933 2985 3063 3117 4136 4493 5109
Size: 831 749 52 78 54 1019 357 616
Cuts at: 5109 5746 1353
Size: 637 3423
Fragments arranged by size:
3423 1019 831 749 637 616 357 78
54 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2569 3875 6403 6777 6923 387
Size: 1064 138 980 1306 2528 374 146 1280
Fragments arranged by size:
2528 1306 1280 1064 980 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2271 2389 2783 3460 3522 3567
Size: 393 596 43 118 394 677 62 45
Cuts at: 3567 3899 5879 6476 7263 7531 1239
Size: 332 1980 597 787 268 1524
Fragments arranged by size:
1980 1524 787 677 597 596 394 393
332 268 118 62 45 43
EcoNI CCTnn'n_nnAGG
Cuts at: 5092 5092
Size: 7816
EcoRI G'AATT_C
Cuts at: 5125 5503 5125
Size: 378 7438
EcoRV GAT'ATC
Cuts at: 2464 2464
Size: 7816
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3190 5056 3190
Size: 1866 5950
FspI TGC'GCA
Cuts at: 6322 6322
Size: 7816
HaeII r_GCGC'y
Cuts at: 1678 3523 6963 1678
Size: 1845 3440 2531
Fragments arranged by size:
3440 2531 1845
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2036 2068 2639 2671 3653
Size: 32 135 32 464 32 571 32 982
Cuts at: 3653 3685 4664 4696 4934 4966 6457 6489 6531
Size: 32 979 32 238 32 1491 32 42
Cuts at: 6531 6563 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 982 979 571 464 238 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2132 2515 5160 5406 669
Size: 988 281 194 383 2645 246 3079
Fragments arranged by size:
3079 2645 988 383 281 246 194
HindIII A'AGCT_T
Cuts at: 748 3172 748
Size: 2424 5392
HpaI GTT'AAC
Cuts at: 5406 5406
Size: 7816
KpnI G_GTAC'C
Cuts at: 5141 5141
Size: 7816
MfeI C'AATT_G
Cuts at: 5415 5415
Size: 7816
MluI A'CGCG_T
Cuts at: 7692 7692
Size: 7816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2077 6930 7114 1305
Size: 772 4853 184 2007
Fragments arranged by size:
4853 2007 772 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3611 4236 10
Size: 54 1425 343 1779 625 3590
Fragments arranged by size:
3590 1779 1425 625 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 3831 4971 5774 6133 6292 518
Size: 648 1512 1153 1140 803 359 159 2042
Fragments arranged by size:
2042 1512 1153 1140 803 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2536 2833 5232 5909 7057 7302 1606
Size: 930 297 2399 677 1148 245 2120
Fragments arranged by size:
2399 2120 1148 930 677 297 245
NaeI GCC'GGC
Cuts at: 3849 3849
Size: 7816
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6325
Fragments arranged by size:
6325 939 552
NdeI CA'TA_TG
Cuts at: 387 4837 387
Size: 4450 3366
NgoMIV G'CCGG_C
Cuts at: 3847 3847
Size: 7816
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7816
NotI GC'GGCC_GC
Cuts at: 5263 5263
Size: 7816
NsiI A_TGCA'T
Cuts at: 2715 2715
Size: 7816
NspI r_CATG'y
Cuts at: 1132 1229 2138 2713 2830 4712 5174 6719 1132
Size: 97 909 575 117 1882 462 1545 2229
Fragments arranged by size:
2229 1882 1545 909 575 462 117 97
PasI CC'CwG_GG
Cuts at: 1348 3446 1348
Size: 2098 5718
PciI A'CATG_T
Cuts at: 2134 4708 6715 2134
Size: 2574 2007 3235
Fragments arranged by size:
3235 2574 2007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7816
PfoI T'CCnGG_A
Cuts at: 2287 3147 2287
Size: 860 6956
PmeI GTTT'AAAC
Cuts at: 5120 5120
Size: 7816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3232 3264 4712 4744 5885 5917 7398 7430 3232
Size: 32 1448 32 1141 32 1481 32 3618
Fragments arranged by size:
3618 1481 1448 1141 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7816
PsiI TTA'TAA
Cuts at: 2870 4452 5386 2870
Size: 1582 934 5300
Fragments arranged by size:
5300 1582 934
PstI C_TGCA'G
Cuts at: 830 3084 3288 5168 830
Size: 2254 204 1880 3478
Fragments arranged by size:
3478 2254 1880 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7816
PvuII CAG'CTG
Cuts at: 2536 2536
Size: 7816
SalI G'TCGA_C
Cuts at: 1936 2130 5158 1936
Size: 194 3028 4594
Fragments arranged by size:
4594 3028 194
SapI GCTCTTCn'nnn_
Cuts at: 3117 3117
Size: 7816
SbfI CC_TGCA'GG
Cuts at: 5168 5168
Size: 7816
ScaI AGT'ACT
Cuts at: 6064 7776 6064
Size: 1712 6104
SfcI C'TryA_G
Cuts at: 826 1045 2841 3080 3131 3284 3968 4865 5164
Size: 219 1796 239 51 153 684 897 299
Cuts at: 5164 6299 6980 7171 826
Size: 1135 681 191 1471
Fragments arranged by size:
1796 1471 1135 897 684 681 299 239
219 191 153 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7816
SmaI CCC'GGG
Cuts at: 1872 3436 5143 1872
Size: 1564 1707 4545
Fragments arranged by size:
4545 1707 1564
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 3575 4292 5920 6821 7083
Size: 197 926 966 666 717 1628 901 262
Cuts at: 7083 820
Size: 1553
Fragments arranged by size:
1628 1553 966 926 901 717 666 262
197
SnaBI TAC'GTA
Cuts at: 493 5607 493
Size: 5114 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7816
SphI G_CATG'C
Cuts at: 1132 5174 1132
Size: 4042 3774
SspI AAT'ATT
Cuts at: 5 52 5740 5
Size: 47 5688 2081
Fragments arranged by size:
5688 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7816
StyI C'CwwG_G
Cuts at: 513 1065 2004 3864 4237 4851 5255 513
Size: 552 939 1860 373 614 404 3074
Fragments arranged by size:
3074 1860 939 614 552 404 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3231 5043 5843 5860 6013 6198 6643
Size: 1255 1651 1812 800 17 153 185 445
Cuts at: 6643 325
Size: 1498
Fragments arranged by size:
1812 1651 1498 1255 800 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 4444 6062
Size: 274 80 33 51 1443 993 1474 1618
Cuts at: 6062 7774 96
Size: 1712 138
Fragments arranged by size:
1712 1618 1474 1443 993 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3914 3946 3981 4013 7776 7808 3914
Size: 32 35 32 3763 32 3922
Fragments arranged by size:
3922 3763 35 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3895 1316
Size: 2579 5237
XbaI T'CTAG_A
Cuts at: 5152 5152
Size: 7816
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2167 4915 2167
Size: 2748 5068
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7816
XmaI C'CCGG_G
Cuts at: 1870 3434 5141 1870
Size: 1564 1707 4545
Fragments arranged by size:
4545 1707 1564
XmnI GAAnn'nnTTC
Cuts at: 5945 5945
Size: 7816
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5624 276
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoO109I FseI FspAI
KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI