Restriction map for
pFN21AA0682
(Circular) (Six-base) MAPSORT of: pFN21AA0682.seq Check: 5275 from: 1 to: 7585
pFN21AA0682 7585 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2888 2888
Size: 7585
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5395 278
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4928 1937
Size: 2991 4594
Acc65I G'GTAC_C
Cuts at: 4906 4906
Size: 7585
AclI AA'CG_TT
Cuts at: 1563 5712 6085 1563
Size: 4149 373 3063
Fragments arranged by size:
4149 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2405 3718 3904 4017 4116 4262 4543
Size: 491 282 1313 186 113 99 146 281
Cuts at: 4543 4862 5648 7032 7300 1632
Size: 319 786 1384 268 1917
Fragments arranged by size:
1917 1384 1313 786 491 319 282 281
268 186 146 113 99
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7388
AflIII A'CryG_T
Cuts at: 1203 3426 6484 7461 1203
Size: 2223 3058 977 1327
Fragments arranged by size:
3058 2223 1327 977
AgeI A'CCGG_T
Cuts at: 6416 6416
Size: 7585
AhdI GACnn_n'nnGTC
Cuts at: 6314 6314
Size: 7585
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2085 1184
Size: 901 6684
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2384 2416 2507 2539 2384
Size: 32 91 32 7430
Fragments arranged by size:
7430 91 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2268 4076 4759 5328 6900 2268
Size: 1808 683 569 1572 2953
Fragments arranged by size:
2953 1808 1572 683 569
ApaLI G'TGCA_C
Cuts at: 2705 3129 5642 6798 2705
Size: 424 2513 1156 3492
Fragments arranged by size:
3492 2513 1156 424
ApoI r'AATT_y
Cuts at: 1493 2939 4894 5120 5272 7381 1493
Size: 1446 1955 226 152 2109 1697
Fragments arranged by size:
2109 1955 1697 1446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6139 160
Size: 872 5107 1606
Fragments arranged by size:
5107 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 4910 1870
Size: 73 1227 1740 4545
Fragments arranged by size:
4545 1740 1227 73
BamHI G'GATC_C
Cuts at: 4915 5282 4915
Size: 367 7218
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2947 3794 4906 6361 618
Size: 325 217 627 1160 847 1112 1455 1842
Fragments arranged by size:
1842 1455 1160 1112 847 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2478 2908 3297 3900 3976 4557 4794 4904
Size: 1087 430 389 603 76 581 237 110
Cuts at: 4904 1391
Size: 4072
Fragments arranged by size:
4072 1087 603 581 430 389 237 110
76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3979 4586 4775 928
Size: 971 2080 607 189 3738
Fragments arranged by size:
3738 2080 971 607 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2224 2258 5776 5810 1903
Size: 34 287 34 3518 34 3678
Fragments arranged by size:
3678 3518 287 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2386 2999 3159 5477 6687 2386
Size: 613 160 2318 1210 3284
Fragments arranged by size:
3284 2318 1210 613 160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2174 2450 3834
Size: 107 122 71 631 605 502 276 1384
Cuts at: 3834 3914 6196 136
Size: 80 2282 1525
Fragments arranged by size:
2282 1525 1384 631 605 502 276 122
107 80 71
BglII A'GATC_T
Cuts at: 7580 7580
Size: 7585
BlpI GC'TnA_GC
Cuts at: 2472 5002 2472
Size: 2530 5055
Bme1580I G_kGCm'C
Cuts at: 1790 2709 3133 3475 5646 6802 1790
Size: 919 424 342 2171 1156 2573
Fragments arranged by size:
2573 2171 1156 919 424 342
BmgBI CAC'GTC
Cuts at: 1318 4396 1318
Size: 3078 4507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2750 4617 5316
Size: 416 53 83 399 24 1333 1867 699
Cuts at: 5316 6274 442
Size: 958 1753
Fragments arranged by size:
1867 1753 1333 958 699 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 3003 2761
Size: 242 7343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2290 2313 2396 3278 3914 3923 3960 3976
Size: 1051 23 83 882 636 9 37 16
Cuts at: 3976 3986 4370 4745 4853 6245 1239
Size: 10 384 375 108 1392 2579
Fragments arranged by size:
2579 1392 1051 882 636 384 375 108
83 37 23 16 10 9
Bpu10I CC'TnA_GC
Cuts at: 3363 3363
Size: 7585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4361 5710 6575 6873 4361
Size: 1349 865 298 5073
Fragments arranged by size:
5073 1349 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2815 3626 3812 6248 882
Size: 617 1316 811 186 2436 2219
Fragments arranged by size:
2436 2219 1316 811 617 186
BsaAI yAC'GTr
Cuts at: 493 1204 3427 5376 493
Size: 711 2223 1949 2702
Fragments arranged by size:
2702 2223 1949 711
BsaBI GATnn'nnATC
Cuts at: 4101 7579 4101
Size: 3478 4107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3984 5392 5774 6409
Size: 53 83 186 936 2451 1408 382 635
Cuts at: 6409 275
Size: 1451
Fragments arranged by size:
2451 1451 1408 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2827 3206 6018 6416 6690 6837 7248 2827
Size: 379 2812 398 274 147 411 3164
Fragments arranged by size:
3164 2812 411 398 379 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2399 2429 2507 2537 1178
Size: 30 165 30 996 30 78 30 6226
Fragments arranged by size:
6226 996 165 78 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2561 2564 3200 3203 3263 4163 4166
Size: 877 498 3 636 3 60 900 3
Cuts at: 4166 4169 4880 1186
Size: 3 711 3891
Fragments arranged by size:
3891 900 877 711 636 498 60 3
3 3 3
BseYI C'CCAG_C
Cuts at: 2657 2810 3675 4054 6788 7284 2657
Size: 153 865 379 2734 496 2958
Fragments arranged by size:
2958 2734 865 496 379 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2627 2805 1163
Size: 573 891 178 5943
Fragments arranged by size:
5943 891 573 178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3807 3829 4776 5035 5796 6824
Size: 275 186 1806 22 947 259 761 1028
Cuts at: 6824 1540
Size: 2301
Fragments arranged by size:
2301 1806 1028 947 761 275 259 186
22
BsiHKAI G_wGCw'C
Cuts at: 2709 3133 3297 3369 4317 4557 5646 5731 6802
Size: 424 164 72 948 240 1089 85 1071
Cuts at: 6802 2709
Size: 3492
Fragments arranged by size:
3492 1089 1071 948 424 240 164 85
72
BsmI GAATG_Cn'
Cuts at: 3341 3574 5096 5189 3341
Size: 233 1522 93 5737
Fragments arranged by size:
5737 1522 233 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2478 2709 2908 3133 3297 3369 3475
Size: 399 688 231 199 225 164 72 106
Cuts at: 3475 3900 3976 4317 4557 4794 4904 5646 5731
Size: 425 76 341 240 237 110 742 85
Cuts at: 5731 6802 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 688 425 399 341 240
237 231 225 199 164 110 106 85
76 72
BspEI T'CCGG_A
Cuts at: 3206 7248 3206
Size: 4042 3543
BspHI T'CATG_A
Cuts at: 5474 7204 5474
Size: 1730 5855
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2357 2888 3237 3391 4940 844
Size: 1513 531 349 154 1549 3489
Fragments arranged by size:
3489 1549 1513 531 349 154
BsrBI CCG'CTC
Cuts at: 1394 1823 4778 5472 1394
Size: 429 2955 694 3507
Fragments arranged by size:
3507 2955 694 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3034 6080 6254 65
Size: 1183 1786 3046 174 1396
Fragments arranged by size:
3046 1786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2497 6229 6416 1700
Size: 110 687 3732 187 2869
Fragments arranged by size:
3732 2869 687 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7585
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7585
BssSI C'ACGA_G
Cuts at: 3629 4787 5645 6657 3629
Size: 1158 858 1012 4557
Fragments arranged by size:
4557 1158 1012 858
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2803 4759 794
Size: 2009 1956 3620
Fragments arranged by size:
3620 2009 1956
BstBI TT'CG_AA
Cuts at: 2971 6389 2971
Size: 3418 4167
BstEII G'GTnAC_C
Cuts at: 1184 4744 1184
Size: 3560 4025
BstXI CCAn_nnnn'nTGG
Cuts at: 3475 4507 3475
Size: 1032 6553
BstYI r'GATC_y
Cuts at: 1972 3253 4425 4503 4545 4827 4915 5282 5667
Size: 1281 1172 78 42 282 88 367 385
Cuts at: 5667 5684 7136 7222 7234 7580 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1281 1172 385 367 346 282
88 86 78 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3573 4041 4323 513
Size: 552 2508 468 282 3775
Fragments arranged by size:
3775 2508 552 468 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3571 487
Size: 683 56 2345 4501
Fragments arranged by size:
4501 2345 683 56
BtsI GCAGTG_nn'
Cuts at: 3873 4855 5101 5895 5915 3873
Size: 982 246 794 20 5543
Fragments arranged by size:
5543 982 794 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7585
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7550
DraI TTT'AAA
Cuts at: 4889 4889
Size: 7585
DraIII CAC_nnn'GTG
Cuts at: 2890 3373 4399 2890
Size: 483 1026 6076
Fragments arranged by size:
6076 1026 483
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3716 6592 809
Size: 507 2400 2876 1802
Fragments arranged by size:
2876 2400 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2499 2964
Size: 54 1425 50 275 18 423 246 465
Cuts at: 2964 3198 3576 3648 3804 3826 4405 4773 5032
Size: 234 378 72 156 22 579 368 259
Cuts at: 5032 5921 7550 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 579 465 423 378 368
275 259 246 234 156 72 54 50
43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 3804 3826 4773 5032 1537
Size: 275 1992 22 947 259 4090
Fragments arranged by size:
4090 1992 947 275 259 22
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2483 2571 2674 2676 2730 3027 3177
Size: 804 326 88 103 2 54 297 150
Cuts at: 3177 3272 4135 4140 4155 4170 4227 4268 4562
Size: 95 863 5 15 15 57 41 294
Cuts at: 4562 4661 5515 1353
Size: 99 854 3423
Fragments arranged by size:
3423 863 854 804 326 297 294 150
103 99 95 88 57 54 41 15
15 5 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2737 3882 4577 4726 4780 6172
Size: 1064 138 1148 1145 695 149 54 1392
Cuts at: 6172 6546 6692 387
Size: 374 146 1280
Fragments arranged by size:
1392 1280 1148 1145 1064 695 374 149
146 138 54
EcoICRI GAG'CTC
Cuts at: 3295 4555 3295
Size: 1260 6325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2290 2313 2396 2405 3278 3718
Size: 393 491 167 23 83 9 873 440
Cuts at: 3718 3904 3914 3923 3960 3976 3986 4017 4116
Size: 186 10 9 37 16 10 31 99
Cuts at: 4116 4262 4370 4543 4745 4853 4862 5648 6245
Size: 146 108 173 202 108 9 786 597
Cuts at: 6245 7032 7300 1239
Size: 787 268 1524
Fragments arranged by size:
1524 873 787 786 597 491 440 393
268 202 186 173 167 146 108 108
99 83 37 31 23 16 10 10
9 9 9
EcoO109I rG'GnC_Cy
Cuts at: 2248 2566 3193 4051 4756 2248
Size: 318 627 858 705 5077
Fragments arranged by size:
5077 858 705 627 318
EcoRI G'AATT_C
Cuts at: 2939 4894 5272 2939
Size: 1955 378 5252
Fragments arranged by size:
5252 1955 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4690 4690
Size: 7585
FspI TGC'GCA
Cuts at: 6091 6091
Size: 7585
HaeII r_GCGC'y
Cuts at: 1678 3085 6732 1678
Size: 1407 3647 2531
Fragments arranged by size:
3647 2531 1407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 4447 4479 4762
Size: 32 135 32 827 32 2016 32 283
Cuts at: 4762 4794 6226 6258 6300 6332 1373
Size: 32 1432 32 42 32 2626
Fragments arranged by size:
2626 2016 1432 827 283 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2372 4929 5175 669
Size: 988 281 434 2557 246 3079
Fragments arranged by size:
3079 2557 988 434 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7585
HpaI GTT'AAC
Cuts at: 5175 5175
Size: 7585
KpnI G_GTAC'C
Cuts at: 4910 4910
Size: 7585
MfeI C'AATT_G
Cuts at: 5184 5184
Size: 7585
MluI A'CGCG_T
Cuts at: 7461 7461
Size: 7585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2601 4130 4800 6699 6883 1305
Size: 1296 1529 670 1899 184 2007
Fragments arranged by size:
2007 1899 1529 1296 670 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2966 10
Size: 54 1425 343 1134 4629
Fragments arranged by size:
4629 1425 1134 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2712 3014 3092 3353 5543 5902 6061
Size: 648 1546 302 78 261 2190 359 159
Cuts at: 6061 518
Size: 2042
Fragments arranged by size:
2190 2042 1546 648 359 302 261 159
78
MspA1I CmG'CkG
Cuts at: 1606 2065 2603 2810 4840 4882 5001 5678 6826
Size: 459 538 207 2030 42 119 677 1148
Cuts at: 6826 7071 1606
Size: 245 2120
Fragments arranged by size:
2120 2030 1148 677 538 459 245 207
119 42
NaeI GCC'GGC
Cuts at: 2499 2499
Size: 7585
NcoI C'CATG_G
Cuts at: 513 1065 4041 4323 513
Size: 552 2976 282 3775
Fragments arranged by size:
3775 2976 552 282
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7585
NgoMIV G'CCGG_C
Cuts at: 2497 2497
Size: 7585
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7585
NotI GC'GGCC_GC
Cuts at: 5032 5032
Size: 7585
NruI TCG'CGA
Cuts at: 2011 2011
Size: 7585
NspI r_CATG'y
Cuts at: 1132 1229 2282 4943 6488 1132
Size: 97 1053 2661 1545 2229
Fragments arranged by size:
2661 2229 1545 1053 97
PasI CC'CwG_GG
Cuts at: 1348 3475 1348
Size: 2127 5458
PciI A'CATG_T
Cuts at: 6484 6484
Size: 7585
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2268 3153 4414 1108
Size: 1160 885 1261 4279
Fragments arranged by size:
4279 1261 1160 885
PfoI T'CCnGG_A
Cuts at: 2213 2403 3684 3891 4830 2213
Size: 190 1281 207 939 4968
Fragments arranged by size:
4968 1281 939 207 190
PmeI GTTT'AAAC
Cuts at: 4889 4889
Size: 7585
PmlI CAC'GTG
Cuts at: 3427 3427
Size: 7585
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 5654 5686 7167 7199 2507
Size: 32 3115 32 1481 32 2893
Fragments arranged by size:
3115 2893 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2566 3193 4051 2248
Size: 318 627 858 5782
Fragments arranged by size:
5782 858 627 318
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7585
PsiI TTA'TAA
Cuts at: 5155 5155
Size: 7585
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 7553
PstI C_TGCA'G
Cuts at: 830 2099 2766 3748 3816 4755 4937 830
Size: 1269 667 982 68 939 182 3478
Fragments arranged by size:
3478 1269 982 939 667 182 68
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7585
PvuII CAG'CTG
Cuts at: 2065 2603 2810 4840 4882 2065
Size: 538 207 2030 42 4768
Fragments arranged by size:
4768 2030 538 207 42
RsrII CG'GwC_CG
Cuts at: 4585 4585
Size: 7585
SacI G_AGCT'C
Cuts at: 3297 4557 3297
Size: 1260 6325
SalI G'TCGA_C
Cuts at: 1936 4927 1936
Size: 2991 4594
SanDI GG'GwC_CC
Cuts at: 2248 2248
Size: 7585
SapI GCTCTTCn'nnn_
Cuts at: 2483 3272 4268 4562 2483
Size: 789 996 294 5506
Fragments arranged by size:
5506 996 789 294
SbfI CC_TGCA'GG
Cuts at: 2766 4755 4937 2766
Size: 1989 182 5414
Fragments arranged by size:
5414 1989 182
ScaI AGT'ACT
Cuts at: 5833 7545 5833
Size: 1712 5873
SfcI C'TryA_G
Cuts at: 826 1045 2095 2762 3744 3812 4751 4933 6068
Size: 219 1050 667 982 68 939 182 1135
Cuts at: 6068 6749 6940 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1050 982 939 681 667 219
191 182 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 3914 3914
Size: 7585
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7585
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7585
SmaI CCC'GGG
Cuts at: 1872 4912 1872
Size: 3040 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4376 5689 6590 6852 820
Size: 197 926 2433 1313 901 262 1553
Fragments arranged by size:
2433 1553 1313 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5376 493
Size: 4883 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7585
SphI G_CATG'C
Cuts at: 1132 4943 1132
Size: 3811 3774
SspI AAT'ATT
Cuts at: 5 52 5509 5
Size: 47 5457 2081
Fragments arranged by size:
5457 2081 47
StuI AGG'CCT
Cuts at: 1517 3380 3917 1517
Size: 1863 537 5185
Fragments arranged by size:
5185 1863 537
StyI C'CwwG_G
Cuts at: 513 1065 3123 3330 3636 4041 4323 5024 513
Size: 552 2058 207 306 405 282 701 3074
Fragments arranged by size:
3074 2058 701 552 405 306 282 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3065 4712 5612 5629 5782 5967 6412
Size: 1255 1485 1647 900 17 153 185 445
Cuts at: 6412 325
Size: 1498
Fragments arranged by size:
1647 1498 1485 1255 900 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3069 3597 4193
Size: 274 80 33 51 1443 1092 528 596
Cuts at: 4193 5831 7543 96
Size: 1638 1712 138
Fragments arranged by size:
1712 1638 1443 1092 596 528 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7545 7577 7545
Size: 32 7553
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7585
XbaI T'CTAG_A
Cuts at: 4921 4921
Size: 7585
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3643 3880 3643
Size: 237 7348
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7585
XmaI C'CCGG_G
Cuts at: 1870 4910 1870
Size: 3040 4545
XmnI GAAnn'nnTTC
Cuts at: 2186 3266 5714 2186
Size: 1080 2448 4057
Fragments arranged by size:
4057 2448 1080
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5393 276
Size: 53 83 186 936 3859 2468
Fragments arranged by size:
3859 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NarI NsiI PacI PspOMI PspXI SacII SexAI
SfoI SrfI SwaI