Restriction map for
pFN21AA0675
(Circular) (Six-base) MAPSORT of: pFN21AA0675.seq Check: 3651 from: 1 to: 8329
pFN21AA0675 8329 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6139 278
Size: 53 83 186 936 4603 2468
Fragments arranged by size:
4603 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3705 4102 4731 5672 1937
Size: 1768 397 629 941 4594
Fragments arranged by size:
4594 1768 941 629 397
Acc65I G'GTAC_C
Cuts at: 5650 5650
Size: 8329
AclI AA'CG_TT
Cuts at: 1563 6456 6829 1563
Size: 4893 373 3063
Fragments arranged by size:
4893 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 6392 7776 8044 1632
Size: 4760 1384 268 1917
Fragments arranged by size:
4760 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8132
AflIII A'CryG_T
Cuts at: 1203 5552 7228 8205 1203
Size: 4349 1676 977 1327
Fragments arranged by size:
4349 1676 1327 977
AgeI A'CCGG_T
Cuts at: 7160 7160
Size: 8329
AhdI GACnn_n'nnGTC
Cuts at: 7058 7058
Size: 8329
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8329
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4842 1184
Size: 3658 4671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 8297
AlwNI CAG_nnn'CTG
Cuts at: 2474 5045 6072 7644 2474
Size: 2571 1027 1572 3159
Fragments arranged by size:
3159 2571 1572 1027
ApaLI G'TGCA_C
Cuts at: 6386 7542 6386
Size: 1156 7173
ApoI r'AATT_y
Cuts at: 1493 2024 2483 2520 2606 2871 2893 3009 3055
Size: 531 459 37 86 265 22 116 46
Cuts at: 3055 3131 5507 5591 5638 5864 6016 8125 1493
Size: 76 2376 84 47 226 152 2109 1697
Fragments arranged by size:
2376 2109 1697 531 459 265 226 152
116 86 84 76 47 46 37 22
AseI AT'TA_AT
Cuts at: 160 1032 2187 2391 3903 6883 160
Size: 872 1155 204 1512 2980 1606
Fragments arranged by size:
2980 1606 1512 1155 872 204
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8329
AvaI C'yCGr_G
Cuts at: 1870 1943 4972 5654 1870
Size: 73 3029 682 4545
Fragments arranged by size:
4545 3029 682 73
AvrII C'CTAG_G
Cuts at: 3709 3709
Size: 8329
BamHI G'GATC_C
Cuts at: 5659 6026 5659
Size: 367 7962
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5650 7105 618
Size: 325 217 627 3863 1455 1842
Fragments arranged by size:
3863 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4208 5008 5035 5648 1391
Size: 2817 800 27 613 4072
Fragments arranged by size:
4072 2817 800 613 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4001 4035 4158 4912 928
Size: 971 2102 34 123 754 4345
Fragments arranged by size:
4345 2102 971 754 123 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6520 6554 1903
Size: 34 4583 34 3678
Fragments arranged by size:
4583 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2913 6221 7431 2913
Size: 3308 1210 3811
Fragments arranged by size:
3811 3308 1210
BclI T'GATC_A
Cuts at: 1548 2917 1548
Size: 1369 6960
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4751 6940 136
Size: 107 122 71 631 605 3079 2189 1525
Fragments arranged by size:
3079 2189 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2701 3955 4496 8324 2701
Size: 1254 541 3828 2706
Fragments arranged by size:
3828 2706 1254 541
BlpI GC'TnA_GC
Cuts at: 4207 5746 4207
Size: 1539 6790
Bme1580I G_kGCm'C
Cuts at: 1790 6390 7546 1790
Size: 4600 1156 2573
Fragments arranged by size:
4600 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2558 1318
Size: 1240 7089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3921 4866 5061
Size: 416 53 83 399 24 2504 945 195
Cuts at: 5061 5242 6060 7018 442
Size: 181 818 958 1753
Fragments arranged by size:
2504 1753 958 945 818 416 399 195
181 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2453 3694 4222 2453
Size: 1241 528 6560
Fragments arranged by size:
6560 1241 528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3944 4830 5386 5457 6989 1239
Size: 2705 886 556 71 1532 2579
Fragments arranged by size:
2705 2579 1532 886 556 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4852 6454 7319 7617 4852
Size: 1602 865 298 5564
Fragments arranged by size:
5564 1602 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3569 6992 882
Size: 617 2070 3423 2219
Fragments arranged by size:
3423 2219 2070 617
BsaAI yAC'GTr
Cuts at: 493 1204 4549 6120 493
Size: 711 3345 1571 2702
Fragments arranged by size:
3345 2702 1571 711
BsaBI GATnn'nnATC
Cuts at: 2504 3690 8323 2504
Size: 1186 4633 2510
Fragments arranged by size:
4633 2510 1186
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6136 6518 7153 275
Size: 53 83 186 936 4603 382 635 1451
Fragments arranged by size:
4603 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6762 7160 7434 7581 7992 6762
Size: 398 274 147 411 7099
Fragments arranged by size:
7099 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4828 4858 5043 5073 1178
Size: 30 165 30 3425 30 185 30 4434
Fragments arranged by size:
4434 3425 185 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3249 4851 1186
Size: 2063 1602 4664
Fragments arranged by size:
4664 2063 1602
BseYI C'CCAG_C
Cuts at: 5346 5606 7532 8028 5346
Size: 260 1926 496 5647
Fragments arranged by size:
5647 1926 496 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2860 3432 5091 5584 1163
Size: 573 1124 572 1659 493 3908
Fragments arranged by size:
3908 1659 1124 573 572 493
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5779 6540 7568 1540
Size: 275 186 3778 761 1028 2301
Fragments arranged by size:
3778 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2578 6390 6475 7546 2578
Size: 3812 85 1071 3361
Fragments arranged by size:
3812 3361 1071 85
BsmI GAATG_Cn'
Cuts at: 4747 4775 5840 5933 4747
Size: 28 1065 93 7143
Fragments arranged by size:
7143 1065 93 28
BsmBI CGTCTCn'nnnn_
Cuts at: 4987 5046 4987
Size: 59 8270
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2578 4208 5008 5035 5648 6390 6475
Size: 399 788 1630 800 27 613 742 85
Cuts at: 6475 7546 1391
Size: 1071 2174
Fragments arranged by size:
2174 1630 1071 800 788 742 613 399
85 27
BspEI T'CCGG_A
Cuts at: 7992 7992
Size: 8329
BspHI T'CATG_A
Cuts at: 5097 5458 6218 7948 5097
Size: 361 760 1730 5478
Fragments arranged by size:
5478 1730 760 361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5063 5684 844
Size: 4219 621 3489
Fragments arranged by size:
4219 3489 621
BsrBI CCG'CTC
Cuts at: 1394 1823 6216 1394
Size: 429 4393 3507
Fragments arranged by size:
4393 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2990 4152 6824 6998 65
Size: 1183 1742 1162 2672 174 1396
Fragments arranged by size:
2672 1742 1396 1183 1162 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5610 6973 7160 1700
Size: 110 3800 1363 187 2869
Fragments arranged by size:
3800 2869 1363 187 110
BsrGI T'GTAC_A
Cuts at: 96 2957 96
Size: 2861 5468
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8329
BssSI C'ACGA_G
Cuts at: 6389 7401 6389
Size: 1012 7317
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8329
BstBI TT'CG_AA
Cuts at: 7133 7133
Size: 8329
BstEII G'GTnAC_C
Cuts at: 1184 5600 1184
Size: 4416 3913
BstYI r'GATC_y
Cuts at: 1972 2662 2701 3955 4496 5659 6026 6411 6428
Size: 690 39 1254 541 1163 367 385 17
Cuts at: 6428 7880 7966 7978 8324 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1254 1163 690 541 385 367
346 86 39 17 12
Bsu36I CC'TnA_GG
Cuts at: 4781 4781
Size: 8329
BtgI C'CryG_G
Cuts at: 513 1065 2004 5531 513
Size: 552 939 3527 3311
Fragments arranged by size:
3527 3311 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2584 487
Size: 683 56 1358 6232
Fragments arranged by size:
6232 1358 683 56
BtsI GCAGTG_nn'
Cuts at: 5845 6639 6659 5845
Size: 794 20 7515
Fragments arranged by size:
7515 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8329
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2187 2222 529
Size: 35 1623 35 6636
Fragments arranged by size:
6636 1623 35 35
DraI TTT'AAA
Cuts at: 2698 2898 3129 3435 5633 2698
Size: 200 231 306 2198 5394
Fragments arranged by size:
5394 2198 306 231 200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7336 809
Size: 507 6020 1802
Fragments arranged by size:
6020 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4993 5776 6665
Size: 54 1425 50 275 18 3163 783 889
Cuts at: 6665 8294 8
Size: 1629 43
Fragments arranged by size:
3163 1629 1425 889 783 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5776 1537
Size: 275 3964 4090
Fragments arranged by size:
4090 3964 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3804 4053 5421 5433 6259 1353
Size: 2451 249 1368 12 826 3423
Fragments arranged by size:
3423 2451 1368 826 249 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6916 7290 7436 387
Size: 1064 138 5327 374 146 1280
Fragments arranged by size:
5327 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3944 4830 5386 5457 6392 6989 7776
Size: 393 2312 886 556 71 935 597 787
Cuts at: 7776 8044 1239
Size: 268 1524
Fragments arranged by size:
2312 1524 935 886 787 597 556 393
268 71
EcoO109I rG'GnC_Cy
Cuts at: 3915 5387 3915
Size: 1472 6857
EcoRI G'AATT_C
Cuts at: 5591 5638 6016 5591
Size: 47 378 7904
Fragments arranged by size:
7904 378 47
EcoRV GAT'ATC
Cuts at: 3084 3535 3084
Size: 451 7878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2366 2723 3296 4499 2245
Size: 121 357 573 1203 6075
Fragments arranged by size:
6075 1203 573 357 121
FspI TGC'GCA
Cuts at: 6835 6835
Size: 8329
HaeII r_GCGC'y
Cuts at: 1678 5045 7476 1678
Size: 3367 2431 2531
Fragments arranged by size:
3367 2531 2431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3105 3137 3316 3348 3547
Size: 32 135 32 1533 32 179 32 199
Cuts at: 3547 3579 3902 3934 5453 5485 6970 7002 7044
Size: 32 323 32 1519 32 1485 32 42
Cuts at: 7044 7076 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 1519 1485 323 199 179 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2102 2149 5673 5919 669
Size: 988 281 164 47 3524 246 3079
Fragments arranged by size:
3524 3079 988 281 246 164 47
HindIII A'AGCT_T
Cuts at: 748 2617 3164 748
Size: 1869 547 5913
Fragments arranged by size:
5913 1869 547
HpaI GTT'AAC
Cuts at: 2149 5919 2149
Size: 3770 4559
KpnI G_GTAC'C
Cuts at: 5654 5654
Size: 8329
MfeI C'AATT_G
Cuts at: 5928 5928
Size: 8329
MluI A'CGCG_T
Cuts at: 8205 8205
Size: 8329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4718 5582 7443 7627 1305
Size: 3413 864 1861 184 2007
Fragments arranged by size:
3413 2007 1861 864 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4995 10
Size: 54 1425 343 3163 3344
Fragments arranged by size:
3344 3163 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3291 3435 6287 6646 6805 518
Size: 648 2125 144 2852 359 159 2042
Fragments arranged by size:
2852 2125 2042 648 359 159 144
MspA1I CmG'CkG
Cuts at: 1606 4211 5745 6422 7570 7815 1606
Size: 2605 1534 677 1148 245 2120
Fragments arranged by size:
2605 2120 1534 1148 677 245
NaeI GCC'GGC
Cuts at: 5612 5612
Size: 8329
NcoI C'CATG_G
Cuts at: 513 1065 2004 5531 513
Size: 552 939 3527 3311
Fragments arranged by size:
3527 3311 939 552
NdeI CA'TA_TG
Cuts at: 387 4042 387
Size: 3655 4674
NgoMIV G'CCGG_C
Cuts at: 5610 5610
Size: 8329
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8329
NotI GC'GGCC_GC
Cuts at: 5776 5776
Size: 8329
NspI r_CATG'y
Cuts at: 1132 1229 5556 5687 7232 1132
Size: 97 4327 131 1545 2229
Fragments arranged by size:
4327 2229 1545 131 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8329
PciI A'CATG_T
Cuts at: 5552 7228 5552
Size: 1676 6653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8329
PfoI T'CCnGG_A
Cuts at: 3960 3960
Size: 8329
PmeI GTTT'AAAC
Cuts at: 5633 5633
Size: 8329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3563 3595 6398 6430 7911 7943 3563
Size: 32 2803 32 1481 32 3949
Fragments arranged by size:
3949 2803 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3915 5387 3915
Size: 1472 6857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8329
PsiI TTA'TAA
Cuts at: 2817 3174 3425 4109 4278 5899 2817
Size: 357 251 684 169 1621 5247
Fragments arranged by size:
5247 1621 684 357 251 169
PstI C_TGCA'G
Cuts at: 830 3505 4966 5681 830
Size: 2675 1461 715 3478
Fragments arranged by size:
3478 2675 1461 715
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8329
PvuII CAG'CTG
Cuts at: 4211 4211
Size: 8329
SalI G'TCGA_C
Cuts at: 1936 5671 1936
Size: 3735 4594
SapI GCTCTTCn'nnn_
Cuts at: 4053 5433 4053
Size: 1380 6949
SbfI CC_TGCA'GG
Cuts at: 5681 5681
Size: 8329
ScaI AGT'ACT
Cuts at: 6577 8289 6577
Size: 1712 6617
SfcI C'TryA_G
Cuts at: 826 1045 2667 3501 3773 4162 4224 4962 5677
Size: 219 1622 834 272 389 62 738 715
Cuts at: 5677 6812 7493 7684 826
Size: 1135 681 191 1471
Fragments arranged by size:
1622 1471 1135 834 738 715 681 389
272 219 191 62
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8329
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8329
SmaI CCC'GGG
Cuts at: 1872 4974 5656 1872
Size: 3102 682 4545
Fragments arranged by size:
4545 3102 682
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4831 6433 7334 7596 820
Size: 197 926 2888 1602 901 262 1553
Fragments arranged by size:
2888 1602 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4549 6120 493
Size: 4056 1571 2702
Fragments arranged by size:
4056 2702 1571
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8329
SphI G_CATG'C
Cuts at: 1132 5687 1132
Size: 4555 3774
SspI AAT'ATT
Cuts at: 5 52 2395 3237 6253 5
Size: 47 2343 842 3016 2081
Fragments arranged by size:
3016 2343 2081 842 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8329
StyI C'CwwG_G
Cuts at: 513 1065 2004 3622 3709 5531 5768 513
Size: 552 939 1618 87 1822 237 3074
Fragments arranged by size:
3074 1822 1618 939 552 237 87
SwaI ATTT'AAAT
Cuts at: 3435 3435
Size: 8329
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3130 3329 3466 4885 6356 6373 6526
Size: 1255 1550 199 137 1419 1471 17 153
Cuts at: 6526 6711 7156 325
Size: 185 445 1498
Fragments arranged by size:
1550 1498 1471 1419 1255 445 199 185
153 137 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2957 3855 4791
Size: 274 80 33 51 1443 980 898 936
Cuts at: 4791 6575 8287 96
Size: 1784 1712 138
Fragments arranged by size:
1784 1712 1443 980 936 898 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5043 5075 5274 5306 8289 8321 5043
Size: 32 199 32 2983 32 5051
Fragments arranged by size:
5051 2983 199 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4034 1316
Size: 2718 5611
XbaI T'CTAG_A
Cuts at: 4636 5665 4636
Size: 1029 7300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 3334 5361 2393
Size: 941 2027 5361
Fragments arranged by size:
5361 2027 941
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8329
XmaI C'CCGG_G
Cuts at: 1870 4972 5654 1870
Size: 3102 682 4545
Fragments arranged by size:
4545 3102 682
XmnI GAAnn'nnTTC
Cuts at: 2301 2885 4710 6458 2301
Size: 584 1825 1748 4172
Fragments arranged by size:
4172 1825 1748 584
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6137 276
Size: 53 83 186 936 4603 2468
Fragments arranged by size:
4603 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI