Restriction map for
pFN21AA0671
(Circular) (Six-base) MAPSORT of: pFN21AA0671.seq Check: 8329 from: 1 to: 6313
pFN21AA0671 6313 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3474 3474
Size: 6313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3222 4376 5760 6028 1632
Size: 1590 1154 1384 268 1917
Fragments arranged by size:
1917 1590 1384 1154 268
AflII C'TTAA_G
Cuts at: 820 1017 2179 820
Size: 197 1162 4954
Fragments arranged by size:
4954 1162 197
AflIII A'CryG_T
Cuts at: 1203 5212 6189 1203
Size: 4009 977 1327
Fragments arranged by size:
4009 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3230 1184
Size: 2046 4267
AlwNI CAG_nnn'CTG
Cuts at: 2720 4056 5628 2720
Size: 1336 1572 3405
Fragments arranged by size:
3405 1572 1336
ApaLI G'TGCA_C
Cuts at: 3487 4370 5526 3487
Size: 883 1156 4274
Fragments arranged by size:
4274 1156 883
ApoI r'AATT_y
Cuts at: 1493 2224 3622 3848 4000 6109 1493
Size: 731 1398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1398 731 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2642 4867 160
Size: 872 1610 2225 1606
Fragments arranged by size:
2225 1610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 1870
Size: 73 1695 4545
Fragments arranged by size:
4545 1695 73
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3634 5089 618
Size: 325 217 627 1847 1455 1842
Fragments arranged by size:
1847 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2729 3516 3632 1391
Size: 1338 787 116 4072
Fragments arranged by size:
4072 1338 787 116
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5342
BbvCI CC'TCA_GC
Cuts at: 2160 2160
Size: 6313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2545 2977 4205 5415 2545
Size: 432 1228 1210 3443
Fragments arranged by size:
3443 1228 1210 432
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4924 136
Size: 107 122 71 631 605 3252 1525
Fragments arranged by size:
3252 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 3491 4374 5530 1790
Size: 1701 883 1156 2573
Fragments arranged by size:
2573 1701 1156 883
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 2909 3160
Size: 416 53 83 399 24 796 696 251
Cuts at: 3160 3384 4044 5002 442
Size: 224 660 958 1753
Fragments arranged by size:
1753 958 796 696 660 416 399 251
224 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3057 4973 1239
Size: 1818 1916 2579
Fragments arranged by size:
2579 1916 1818
Bpu10I CC'TnA_GC
Cuts at: 2160 3218 2160
Size: 1058 5255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2669 4438 5303 5601 2669
Size: 1769 865 298 3381
Fragments arranged by size:
3381 1769 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4976 882
Size: 617 3477 2219
Fragments arranged by size:
3477 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3495 4104 493
Size: 711 2291 609 2702
Fragments arranged by size:
2702 2291 711 609
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3324 4120 4502 5137
Size: 53 83 186 936 1791 796 382 635
Cuts at: 5137 275
Size: 1451
Fragments arranged by size:
1791 1451 936 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4746 5144 5418 5565 5976 4746
Size: 398 274 147 411 5083
Fragments arranged by size:
5083 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 1178
Size: 30 165 30 684 30 5374
Fragments arranged by size:
5374 684 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2148 3340 1186
Size: 962 1192 4159
Fragments arranged by size:
4159 1192 962
BseYI C'CCAG_C
Cuts at: 5516 6012 5516
Size: 496 5817
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2859 1163
Size: 573 1123 4617
Fragments arranged by size:
4617 1123 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3491 4374 4459 5530 3491
Size: 883 85 1071 4274
Fragments arranged by size:
4274 1071 883 85
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2729 3491 3516 3632 4374 4459 5530
Size: 399 939 762 25 116 742 85 1071
Cuts at: 5530 1391
Size: 2174
Fragments arranged by size:
2174 1071 939 762 742 399 116 85
25
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 2065 4202 5932 2065
Size: 2137 1730 2446
Fragments arranged by size:
2446 2137 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3474 3668 844
Size: 2630 194 3489
Fragments arranged by size:
3489 2630 194
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3413 4808 4982 65
Size: 1183 2165 1395 174 1396
Fragments arranged by size:
2165 1396 1395 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2485 96
Size: 2389 3924
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 3110 4373 5385 3110
Size: 1263 1012 4038
Fragments arranged by size:
4038 1263 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 2219 5117 2219
Size: 2898 3415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 3275 3385 3643 4010 4395 4412 5864 5950
Size: 1303 110 258 367 385 17 1452 86
Cuts at: 5950 5962 6308 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1303 385 367 346 258 110
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2360 513
Size: 552 939 356 4466
Fragments arranged by size:
4466 939 552 356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 3103 3305 3829 4623 4643 3103
Size: 202 524 794 20 4773
Fragments arranged by size:
4773 794 524 202 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 3546 3617 3546
Size: 71 6242
DraIII CAC_nnn'GTG
Cuts at: 2997 2997
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3760 4649 6278
Size: 54 1425 50 275 18 1930 889 1629
Cuts at: 6278 8
Size: 43
Fragments arranged by size:
1930 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 2329 2973 3251 4243 1353
Size: 710 266 644 278 992 3423
Fragments arranged by size:
3423 992 710 644 278 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3251 4900 5274 5420 387
Size: 1064 138 1662 1649 374 146 1280
Fragments arranged by size:
1662 1649 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3057 3222 4376 4973 5760 6028 1239
Size: 393 1425 165 1154 597 787 268 1524
Fragments arranged by size:
1524 1425 1154 787 597 393 268 165
EcoRI G'AATT_C
Cuts at: 2224 3622 4000 2224
Size: 1398 378 4537
Fragments arranged by size:
4537 1398 378
EcoRV GAT'ATC
Cuts at: 3033 3033
Size: 6313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2623 2810 2623
Size: 187 6126
FspI TGC'GCA
Cuts at: 3231 4819 3231
Size: 1588 4725
HaeII r_GCGC'y
Cuts at: 1678 2470 5460 1678
Size: 792 2990 2531
Fragments arranged by size:
2990 2531 792
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2330 2362 3585
Size: 32 135 32 515 32 211 32 1223
Cuts at: 3585 3617 4954 4986 5028 5060 1373
Size: 32 1337 32 42 32 2626
Fragments arranged by size:
2626 1337 1223 515 211 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2130 3567 5427 5611 1305
Size: 825 1437 1860 184 2007
Fragments arranged by size:
2007 1860 1437 825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4491
Fragments arranged by size:
4491 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3051 4271 4630 4789 518
Size: 648 1885 1220 359 159 2042
Fragments arranged by size:
2042 1885 1220 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3729 4406 5554 5799 1606
Size: 2123 677 1148 245 2120
Fragments arranged by size:
2123 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2360 513
Size: 552 939 356 4466
Fragments arranged by size:
4466 939 552 356
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 2357 3287 3403 3671 5216 1132
Size: 97 1128 930 116 268 1545 2229
Fragments arranged by size:
2229 1545 1128 930 268 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2366 1108
Size: 1258 5055
PfoI T'CCnGG_A
Cuts at: 2917 2917
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 3381 3561 3883 3381
Size: 180 322 5811
Fragments arranged by size:
5811 322 180
PstI C_TGCA'G
Cuts at: 830 3459 3485 3665 830
Size: 2629 26 180 3478
Fragments arranged by size:
3478 2629 180 26
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SfcI C'TryA_G
Cuts at: 826 1045 2517 2772 3455 3481 3661 4796 5477
Size: 219 1472 255 683 26 180 1135 681
Cuts at: 5477 5668 826
Size: 191 1471
Fragments arranged by size:
1472 1471 1135 683 681 255 219 191
180 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2179 2648 4417 5318 5580 820
Size: 197 926 236 469 1769 901 262 1553
Fragments arranged by size:
1769 1553 926 901 469 262 236 197
SnaBI TAC'GTA
Cuts at: 493 3495 4104 493
Size: 3002 609 2702
Fragments arranged by size:
3002 2702 609
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3287 3671 1132
Size: 2155 384 3774
Fragments arranged by size:
3774 2155 384
SspI AAT'ATT
Cuts at: 5 52 2191 4237 5
Size: 47 2139 2046 2081
Fragments arranged by size:
2139 2081 2046 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6313
StyI C'CwwG_G
Cuts at: 513 1065 2004 2360 3752 513
Size: 552 939 356 1392 3074
Fragments arranged by size:
3074 1392 939 552 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2051 4340 4357 4510 4695 5140 325
Size: 1255 471 2289 17 153 185 445 1498
Fragments arranged by size:
2289 1498 1255 471 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2485 4559 6271
Size: 274 80 33 51 1443 508 2074 1712
Cuts at: 6271 96
Size: 138
Fragments arranged by size:
2074 1712 1443 508 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6273 6305 6273
Size: 32 6281
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 2033 2057 2338 3183 4442 2033
Size: 24 281 845 1259 3904
Fragments arranged by size:
3904 1259 845 281 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI