Restriction map for
pFN21AA0628
(Circular) (Six-base) MAPSORT of: pFN21AA0628.seq Check: 4441 from: 1 to: 6313
pFN21AA0628 6313 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 6313
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2108 3083 4123 278
Size: 53 83 186 936 572 975 1040 2468
Fragments arranged by size:
2468 1040 975 936 572 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 3507 4440 4813 1563
Size: 1944 933 373 3063
Fragments arranged by size:
3063 1944 933 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 2327 2719 3055 3471 4376 5760 6028
Size: 453 242 392 336 416 905 1384 268
Cuts at: 6028 1632
Size: 1917
Fragments arranged by size:
1917 1384 905 453 416 392 336 268
242
AfeI AGC'GCT
Cuts at: 3068 3068
Size: 6313
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 2457 5212 6189 1203
Size: 1254 2755 977 1327
Fragments arranged by size:
2755 1327 1254 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 2540 5042 2540
Size: 2502 3811
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 4615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6313
AlwNI CAG_nnn'CTG
Cuts at: 2206 2235 4056 5628 2206
Size: 29 1821 1572 2891
Fragments arranged by size:
2891 1821 1572 29
ApaLI G'TGCA_C
Cuts at: 2293 4370 5526 2293
Size: 2077 1156 3080
Fragments arranged by size:
3080 2077 1156
ApoI r'AATT_y
Cuts at: 1493 2517 2937 3105 3441 3622 3848 4000 6109
Size: 1024 420 168 336 181 226 152 2109
Cuts at: 6109 1493
Size: 1697
Fragments arranged by size:
2109 1697 1024 420 336 226 181 168
152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 2830 3638 1870
Size: 73 198 689 808 4545
Fragments arranged by size:
4545 808 689 198 73
BamHI G'GATC_C
Cuts at: 3021 3643 4010 3021
Size: 622 367 5324
Fragments arranged by size:
5324 622 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2676 3634 5089 618
Size: 325 217 627 889 958 1455 1842
Fragments arranged by size:
1842 1455 958 889 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 2166 2181 2355 2495 2747 2831 3335
Size: 742 33 15 174 140 252 84 504
Cuts at: 3335 3632 1391
Size: 297 4072
Fragments arranged by size:
4072 742 504 297 252 174 140 84
33 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4924 136
Size: 107 122 71 631 605 3252 1525
Fragments arranged by size:
3252 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 3730 3730
Size: 6313
Bme1580I G_kGCm'C
Cuts at: 1790 2297 4374 5530 1790
Size: 507 2077 1156 2573
Fragments arranged by size:
2573 2077 1156 507
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3039 4044 5002
Size: 416 53 83 399 24 1622 1005 958
Cuts at: 5002 442
Size: 1753
Fragments arranged by size:
1753 1622 1005 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2552 3140 3559 2152
Size: 400 588 419 4906
Fragments arranged by size:
4906 588 419 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2547 3135 3219 3227 3387 4973 1239
Size: 1308 588 84 8 160 1586 2579
Fragments arranged by size:
2579 1586 1308 588 160 84 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2271 3568 4438 5303 5601 2271
Size: 1297 870 865 298 2983
Fragments arranged by size:
2983 1297 870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2588 2695 2947 4976 882
Size: 617 1089 107 252 2029 2219
Fragments arranged by size:
2219 2029 1089 617 252 107
BsaAI yAC'GTr
Cuts at: 493 1204 2460 3046 4104 493
Size: 711 1256 586 1058 2702
Fragments arranged by size:
2702 1256 1058 711 586
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 3080 4120 4502
Size: 53 83 186 936 572 975 1040 382
Cuts at: 4502 5137 275
Size: 635 1451
Fragments arranged by size:
1451 1040 975 936 635 572 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2945 4746 5144 5418 5565 5976 2945
Size: 1801 398 274 147 411 3282
Fragments arranged by size:
3282 1801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3192 3222 1178
Size: 30 165 30 1789 30 4269
Fragments arranged by size:
4269 1789 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6313
BseYI C'CCAG_C
Cuts at: 2441 3093 5516 6012 2441
Size: 652 2423 496 2742
Fragments arranged by size:
2742 2423 652 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3332 3406 1163
Size: 573 1596 74 4070
Fragments arranged by size:
4070 1596 573 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2181 2297 2355 2495 2747 2831 3267 3335
Size: 48 116 58 140 252 84 436 68
Cuts at: 3335 4374 4459 5530 2133
Size: 1039 85 1071 2916
Fragments arranged by size:
2916 1071 1039 436 252 140 116 85
84 68 58 48
BsmI GAATG_Cn'
Cuts at: 2717 2885 2900 2969 3824 3917 2717
Size: 168 15 69 855 93 5113
Fragments arranged by size:
5113 855 168 93 69 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2166 2181 2297 2355 2495 2747
Size: 399 343 33 15 116 58 140 252
Cuts at: 2747 2831 3267 3335 3632 4374 4459 5530 1391
Size: 84 436 68 297 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 343 297 252
140 116 85 84 68 58 33 15
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 4202 5932 4202
Size: 1730 4583
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 3668 844
Size: 1389 1435 3489
Fragments arranged by size:
3489 1435 1389
BsrBI CCG'CTC
Cuts at: 1394 1823 4200 1394
Size: 429 2377 3507
Fragments arranged by size:
3507 2377 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2806 2974 3058 4808 4982 65
Size: 1183 1558 168 84 1750 174 1396
Fragments arranged by size:
1750 1558 1396 1183 174 168 84
BsrFI r'CCGG_y
Cuts at: 1700 1810 4957 5144 1700
Size: 110 3147 187 2869
Fragments arranged by size:
3147 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 2159 4373 5385 2159
Size: 2214 1012 3087
Fragments arranged by size:
3087 2214 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 3297 5117 3297
Size: 1820 4493
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 3568 3568
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 2265 2853 3021 3273 3643 4010 4395 4412
Size: 293 588 168 252 370 367 385 17
Cuts at: 4412 5864 5950 5962 6308 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 588 385 370 367 346 293
252 168 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2894 487
Size: 683 56 1668 3906
Fragments arranged by size:
3906 1668 683 56
BtsI GCAGTG_nn'
Cuts at: 2642 3829 4623 4643 2642
Size: 1187 794 20 4312
Fragments arranged by size:
4312 1187 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2704 2739 2788 2823 2872 2907 2956
Size: 35 2140 35 49 35 49 35 49
Cuts at: 2956 2991 3040 3075 529
Size: 35 49 35 3767
Fragments arranged by size:
3767 2140 49 49 49 49 35 35
35 35 35 35
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2485 3760 4649
Size: 54 1425 50 275 18 655 1275 889
Cuts at: 4649 6278 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1275 889 655 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3447 4243 1353
Size: 2094 796 3423
Fragments arranged by size:
3423 2094 796
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 2179 2353 2493 2745 2829 3333 2131
Size: 48 174 140 252 84 504 5111
Fragments arranged by size:
5111 504 252 174 140 84 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2327 2547 2719 3055 3135 3219
Size: 393 453 242 220 172 336 80 84
Cuts at: 3219 3227 3387 3471 4376 4973 5760 6028 1239
Size: 8 160 84 905 597 787 268 1524
Fragments arranged by size:
1524 905 787 597 453 393 336 268
242 220 172 160 84 84 80 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2821 2821
Size: 6313
EcoO109I rG'GnC_Cy
Cuts at: 2787 3291 3556 2787
Size: 504 265 5544
Fragments arranged by size:
5544 504 265
EcoRI G'AATT_C
Cuts at: 2517 2937 3105 3622 4000 2517
Size: 420 168 517 378 4830
Fragments arranged by size:
4830 517 420 378 168
EcoRV GAT'ATC
Cuts at: 2383 2383
Size: 6313
FspI TGC'GCA
Cuts at: 2634 4819 2634
Size: 2185 4128
HaeII r_GCGC'y
Cuts at: 1678 3070 5460 1678
Size: 1392 2390 2531
Fragments arranged by size:
2531 2390 1392
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2526 2558 3479
Size: 32 135 32 503 32 419 32 921
Cuts at: 3479 3511 4954 4986 5028 5060 1373
Size: 32 1443 32 42 32 2626
Fragments arranged by size:
2626 1443 921 503 419 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3150 748
Size: 2402 3911
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5427 5611 1305
Size: 4122 184 2007
Fragments arranged by size:
4122 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2487 10
Size: 54 1425 343 655 3836
Fragments arranged by size:
3836 1425 655 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2864 3570 4271 4630 4789 518
Size: 648 957 741 706 701 359 159 2042
Fragments arranged by size:
2042 957 741 706 701 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3729 4406 5554 5799 1606
Size: 2123 677 1148 245 2120
Fragments arranged by size:
2123 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5761
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 2441 3671 5216 1132
Size: 97 1212 1230 1545 2229
Fragments arranged by size:
2229 1545 1230 1212 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PmlI CAC'GTG
Cuts at: 2460 2460
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspXI vC'TCGA_Gb
Cuts at: 2830 2830
Size: 6313
PstI C_TGCA'G
Cuts at: 830 3665 830
Size: 2835 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
SacI G_AGCT'C
Cuts at: 2133 2181 2355 2495 2747 2831 3335 2133
Size: 48 174 140 252 84 504 5111
Fragments arranged by size:
5111 504 252 174 140 84 48
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SfcI C'TryA_G
Cuts at: 826 1045 3582 3661 4796 5477 5668 826
Size: 219 2537 79 1135 681 191 1471
Fragments arranged by size:
2537 1471 1135 681 219 191 79
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2286 2830 3547 4417 5318 5580
Size: 197 926 343 544 717 870 901 262
Cuts at: 5580 820
Size: 1553
Fragments arranged by size:
1553 926 901 870 717 544 343 262
197
SnaBI TAC'GTA
Cuts at: 493 3046 4104 493
Size: 2553 1058 2702
Fragments arranged by size:
2702 2553 1058
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 2441 3671 1132
Size: 1309 1230 3774
Fragments arranged by size:
3774 1309 1230
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 3040 1517
Size: 1523 4790
StyI C'CwwG_G
Cuts at: 513 1065 3752 513
Size: 552 2687 3074
Fragments arranged by size:
3074 2687 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3107 3560 4340 4357 4510 4695 5140
Size: 1255 1527 453 780 17 153 185 445
Cuts at: 5140 325
Size: 1498
Fragments arranged by size:
1527 1498 1255 780 453 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4559 6271 96
Size: 274 80 33 51 1443 2582 1712 138
Fragments arranged by size:
2582 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3192 3224 6273 6305 3192
Size: 32 3049 32 3200
Fragments arranged by size:
3200 3049 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2104 1316
Size: 788 5525
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 2830 1943
Size: 887 5426
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 2564 2732 2816 2900 3068 3152 3320 4442 2564
Size: 168 84 84 168 84 168 1122 4435
Fragments arranged by size:
4435 1122 168 168 168 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2106 3081 4121 276
Size: 53 83 186 936 572 975 1040 2468
Fragments arranged by size:
2468 1040 975 936 572 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI