Restriction map for
pFN21AA0627
(Circular) (Six-base) MAPSORT of: pFN21AA0627.seq Check: 7267 from: 1 to: 8587
pFN21AA0627 8587 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2138 3128 3185 2138
Size: 990 57 7540
Fragments arranged by size:
7540 990 57
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6397 278
Size: 53 83 186 936 4861 2468
Fragments arranged by size:
4861 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 3745 5930 1937
Size: 1086 722 2185 4594
Fragments arranged by size:
4594 2185 1086 722
Acc65I G'GTAC_C
Cuts at: 5908 5908
Size: 8587
AclI AA'CG_TT
Cuts at: 1563 6714 7087 1563
Size: 5151 373 3063
Fragments arranged by size:
5151 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2550 3285 4304 4827 4860 6650 8034
Size: 731 187 735 1019 523 33 1790 1384
Cuts at: 8034 8302 1632
Size: 268 1917
Fragments arranged by size:
1917 1790 1384 1019 735 731 523 268
187 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8390
AflIII A'CryG_T
Cuts at: 1203 7486 8463 1203
Size: 6283 977 1327
Fragments arranged by size:
6283 1327 977
AgeI A'CCGG_T
Cuts at: 7418 7418
Size: 8587
AhdI GACnn_n'nnGTC
Cuts at: 7316 7316
Size: 8587
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5564 1184
Size: 4380 4207
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2125 2157 2125
Size: 32 8555
AlwNI CAG_nnn'CTG
Cuts at: 3263 3791 5846 6330 7902 3263
Size: 528 2055 484 1572 3948
Fragments arranged by size:
3948 2055 1572 528 484
ApaLI G'TGCA_C
Cuts at: 5832 6644 7800 5832
Size: 812 1156 6619
Fragments arranged by size:
6619 1156 812
ApoI r'AATT_y
Cuts at: 1493 2295 2349 2705 2790 4095 4444 4852 5896
Size: 802 54 356 85 1305 349 408 1044
Cuts at: 5896 6122 6274 8383 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1305 1044 802 408 356 349
226 152 85 54
AseI AT'TA_AT
Cuts at: 160 1032 5586 7141 160
Size: 872 4554 1555 1606
Fragments arranged by size:
4554 1606 1555 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8587
AvaI C'yCGr_G
Cuts at: 1870 1943 4473 5052 5912 1870
Size: 73 2530 579 860 4545
Fragments arranged by size:
4545 2530 860 579 73
AvrII C'CTAG_G
Cuts at: 4027 4027
Size: 8587
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2616 2649 2616
Size: 33 8554
BamHI G'GATC_C
Cuts at: 4426 5917 6284 4426
Size: 1491 367 6729
Fragments arranged by size:
6729 1491 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3140 3197 3680 5908 7363
Size: 325 217 627 1353 57 483 2228 1455
Cuts at: 7363 618
Size: 1842
Fragments arranged by size:
2228 1842 1455 1353 627 483 325 217
57
BanII G_rGCy'C
Cuts at: 1391 3437 5906 1391
Size: 2046 2469 4072
Fragments arranged by size:
4072 2469 2046
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2906 3747 5719 928
Size: 971 1007 841 1972 3796
Fragments arranged by size:
3796 1972 1007 971 841
BbvCI CC'TCA_GC
Cuts at: 2808 3469 5493 2808
Size: 661 2024 5902
Fragments arranged by size:
5902 2024 661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6778 6812 1903
Size: 34 4841 34 3678
Fragments arranged by size:
4841 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3226 6479 7689 3226
Size: 3253 1210 4124
Fragments arranged by size:
4124 3253 1210
BclI T'GATC_A
Cuts at: 1548 2518 4351 1548
Size: 970 1833 5784
Fragments arranged by size:
5784 1833 970
BfrBI ATG'CAT
Cuts at: 3862 3890 3862
Size: 28 8559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7198 136
Size: 107 122 71 631 605 5526 1525
Fragments arranged by size:
5526 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5486 8582 5486
Size: 3096 5491
BlpI GC'TnA_GC
Cuts at: 6004 6004
Size: 8587
Bme1580I G_kGCm'C
Cuts at: 1790 3202 3685 5836 6648 7804 1790
Size: 1412 483 2151 812 1156 2573
Fragments arranged by size:
2573 2151 1412 1156 812 483
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8587
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2329 6318 7276
Size: 416 53 83 399 24 912 3989 958
Cuts at: 7276 442
Size: 1753
Fragments arranged by size:
3989 1753 958 912 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8587
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2211 2391 3378 3640 5868 7247 1239
Size: 972 180 987 262 2228 1379 2579
Fragments arranged by size:
2579 2228 1379 987 972 262 180
Bpu10I CC'TnA_GC
Cuts at: 2439 2808 3187 3469 5493 2439
Size: 369 379 282 2024 5533
Fragments arranged by size:
5533 2024 379 369 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2912 4966 5096 6712 7577 7875 2912
Size: 2054 130 1616 865 298 3624
Fragments arranged by size:
3624 2054 1616 865 298 130
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3396 7250 882
Size: 617 1897 3854 2219
Fragments arranged by size:
3854 2219 1897 617
BsaAI yAC'GTr
Cuts at: 493 1204 6378 493
Size: 711 5174 2702
Fragments arranged by size:
5174 2702 711
BsaBI GATnn'nnATC
Cuts at: 3847 8581 3847
Size: 4734 3853
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3876 6394 6776 7411
Size: 53 83 186 936 2343 2518 382 635
Cuts at: 7411 275
Size: 1451
Fragments arranged by size:
2518 2343 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2108 7020 7418 7692 7839 8250 2108
Size: 4912 398 274 147 411 2445
Fragments arranged by size:
4912 2445 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4750 4780 1178
Size: 30 165 30 3347 30 4985
Fragments arranged by size:
4985 3347 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2704 3815 5029 5865 1186
Size: 1518 1111 1214 836 3908
Fragments arranged by size:
3908 1518 1214 1111 836
BseYI C'CCAG_C
Cuts at: 7790 8286 7790
Size: 496 8091
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2079 2361 2619 3213 1163
Size: 573 343 282 258 594 6537
Fragments arranged by size:
6537 594 573 343 282 258
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6037 6798 7826 1540
Size: 275 186 4036 761 1028 2301
Fragments arranged by size:
4036 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2425 3437 5836 6648 6733 7804 2425
Size: 1012 2399 812 85 1071 3208
Fragments arranged by size:
3208 2399 1071 1012 812 85
BsmI GAATG_Cn'
Cuts at: 3133 3211 3862 3864 6098 6191 3133
Size: 78 651 2 2234 93 5529
Fragments arranged by size:
5529 2234 651 93 78 2
BsmBI CGTCTCn'nnnn_
Cuts at: 2686 2686
Size: 8587
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2425 3202 3437 3685 5836 5906 6648
Size: 399 635 777 235 248 2151 70 742
Cuts at: 6648 6733 7804 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2151 1071 777 742 635 399 248
235 85 70
BspEI T'CCGG_A
Cuts at: 2108 8250 2108
Size: 6142 2445
BspHI T'CATG_A
Cuts at: 2794 5439 6476 8206 2794
Size: 2645 1037 1730 3175
Fragments arranged by size:
3175 2645 1730 1037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2138 3128 3185 3200 5658 5942 844
Size: 1294 990 57 15 2458 284 3489
Fragments arranged by size:
3489 2458 1294 990 284 57 15
BsrBI CCG'CTC
Cuts at: 1394 1823 6474 1394
Size: 429 4651 3507
Fragments arranged by size:
4651 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4660 5012 7082 7256 65
Size: 1183 3412 352 2070 174 1396
Fragments arranged by size:
3412 2070 1396 1183 352 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3381 7231 7418 1700
Size: 110 1571 3850 187 2869
Fragments arranged by size:
3850 2869 1571 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8587
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8587
BssSI C'ACGA_G
Cuts at: 6647 7659 6647
Size: 1012 7575
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3155 3302 3896 794
Size: 2361 147 594 5485
Fragments arranged by size:
5485 2361 594 147
BstBI TT'CG_AA
Cuts at: 4642 7391 4642
Size: 2749 5838
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8587
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2861 4423 4716 2011
Size: 850 1562 293 5882
Fragments arranged by size:
5882 1562 850 293
BstYI r'GATC_y
Cuts at: 1972 2443 2450 4256 4426 5464 5486 5917 6284
Size: 471 7 1806 170 1038 22 431 367
Cuts at: 6284 6669 6686 8138 8224 8236 8582 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1806 1452 1038 471 431 385 367
346 170 86 22 17 12 7
Bsu36I CC'TnA_GG
Cuts at: 2157 3712 5393 2157
Size: 1555 1681 5351
Fragments arranged by size:
5351 1681 1555
BtgI C'CryG_G
Cuts at: 513 1065 2004 3494 4671 513
Size: 552 939 1490 1177 4429
Fragments arranged by size:
4429 1490 1177 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3900 487
Size: 683 56 2674 5174
Fragments arranged by size:
5174 2674 683 56
BtsI GCAGTG_nn'
Cuts at: 2357 2947 4473 5543 5713 6103 6897 6917 2357
Size: 590 1526 1070 170 390 794 20 4027
Fragments arranged by size:
4027 1526 1070 794 590 390 170 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8587
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8552
DraI TTT'AAA
Cuts at: 5416 5891 5416
Size: 475 8112
DraIII CAC_nnn'GTG
Cuts at: 3113 3113
Size: 8587
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7594 809
Size: 507 6278 1802
Fragments arranged by size:
6278 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3271 4413 5514
Size: 54 1425 50 275 18 1441 1142 1101
Cuts at: 5514 6034 6923 8552 8
Size: 520 889 1629 43
Fragments arranged by size:
1629 1441 1425 1142 1101 889 520 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6034 1537
Size: 275 4222 4090
Fragments arranged by size:
4222 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2903 2958 3046 3394 6517 1353
Size: 1550 55 88 348 3123 3423
Fragments arranged by size:
3423 3123 1550 348 88 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7174 7548 7694 387
Size: 1064 138 5585 374 146 1280
Fragments arranged by size:
5585 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3435 3435
Size: 8587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2211 2363 2391 2550 3285 3378 3640
Size: 393 579 152 28 159 735 93 262
Cuts at: 3640 4304 4827 4860 5868 6650 7247 8034 8302
Size: 664 523 33 1008 782 597 787 268
Cuts at: 8302 1239
Size: 1524
Fragments arranged by size:
1524 1008 787 782 735 664 597 579
523 393 268 262 159 152 93 33
28
EcoO109I rG'GnC_Cy
Cuts at: 2970 3708 2970
Size: 738 7849
EcoRI G'AATT_C
Cuts at: 2790 5896 6274 2790
Size: 3106 378 5103
Fragments arranged by size:
5103 3106 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4208 4320 4208
Size: 112 8475
FspI TGC'GCA
Cuts at: 7093 7093
Size: 8587
HaeII r_GCGC'y
Cuts at: 1678 2529 3187 7734 1678
Size: 851 658 4547 2531
Fragments arranged by size:
4547 2531 851 658
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2435 2467 2957
Size: 32 135 32 455 32 376 32 490
Cuts at: 2957 2989 3441 3473 5615 5647 7228 7260 7302
Size: 32 452 32 2142 32 1581 32 42
Cuts at: 7302 7334 1373
Size: 32 2626
Fragments arranged by size:
2626 2142 1581 490 455 452 376 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3746 5786 5931 6177 669
Size: 988 281 1808 2040 145 246 3079
Fragments arranged by size:
3079 2040 1808 988 281 246 145
HindIII A'AGCT_T
Cuts at: 748 2026 2220 2469 4369 4622 5007 5718 748
Size: 1278 194 249 1900 253 385 711 3617
Fragments arranged by size:
3617 1900 1278 711 385 253 249 194
HpaI GTT'AAC
Cuts at: 6177 6177
Size: 8587
KpnI G_GTAC'C
Cuts at: 5912 5912
Size: 8587
MfeI C'AATT_G
Cuts at: 2662 4000 6186 2662
Size: 1338 2186 5063
Fragments arranged by size:
5063 2186 1338
MluI A'CGCG_T
Cuts at: 8463 8463
Size: 8587
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2143 2394 7701 7885 1305
Size: 838 251 5307 184 2007
Fragments arranged by size:
5307 2007 838 251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4415 5516 10
Size: 54 1425 343 2583 1101 3081
Fragments arranged by size:
3081 2583 1425 1101 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2628 3122 3867 4106 4185 5546
Size: 648 843 619 494 745 239 79 1361
Cuts at: 5546 6545 6904 7063 518
Size: 999 359 159 2042
Fragments arranged by size:
2042 1361 999 843 745 648 619 494
359 239 159 79
MspA1I CmG'CkG
Cuts at: 1606 2072 6003 6680 7828 8073 1606
Size: 466 3931 677 1148 245 2120
Fragments arranged by size:
3931 2120 1148 677 466 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4671 513
Size: 552 939 2667 4429
Fragments arranged by size:
4429 2667 939 552
NdeI CA'TA_TG
Cuts at: 387 3853 387
Size: 3466 5121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8587
NotI GC'GGCC_GC
Cuts at: 6034 6034
Size: 8587
NsiI A_TGCA'T
Cuts at: 3864 3892 3864
Size: 28 8559
NspI r_CATG'y
Cuts at: 1132 1229 2759 4147 5945 7490 1132
Size: 97 1530 1388 1798 1545 2229
Fragments arranged by size:
2229 1798 1545 1530 1388 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8587
PciI A'CATG_T
Cuts at: 7486 7486
Size: 8587
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3305 3725 1108
Size: 2197 420 5970
Fragments arranged by size:
5970 2197 420
PfoI T'CCnGG_A
Cuts at: 3088 3394 4429 3088
Size: 306 1035 7246
Fragments arranged by size:
7246 1035 306
PmeI GTTT'AAAC
Cuts at: 5891 5891
Size: 8587
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2712 6656 6688 8169 8201 2680
Size: 32 3944 32 1481 32 3066
Fragments arranged by size:
3944 3066 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2970 2970
Size: 8587
PshAI GACnn'nnGTC
Cuts at: 1613 3624 3751 4983 1613
Size: 2011 127 1232 5217
Fragments arranged by size:
5217 2011 1232 127
PsiI TTA'TAA
Cuts at: 2216 4442 6157 2216
Size: 2226 1715 4646
Fragments arranged by size:
4646 2226 1715
PstI C_TGCA'G
Cuts at: 830 3102 3139 4038 5575 5939 830
Size: 2272 37 899 1537 364 3478
Fragments arranged by size:
3478 2272 1537 899 364 37
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8587
PvuII CAG'CTG
Cuts at: 2072 2072
Size: 8587
SacI G_AGCT'C
Cuts at: 3437 3437
Size: 8587
SalI G'TCGA_C
Cuts at: 1936 3744 5929 1936
Size: 1808 2185 4594
Fragments arranged by size:
4594 2185 1808
SbfI CC_TGCA'GG
Cuts at: 5939 5939
Size: 8587
ScaI AGT'ACT
Cuts at: 6835 8547 6835
Size: 1712 6875
SfcI C'TryA_G
Cuts at: 826 1045 2481 3024 3098 3135 3508 3916 4034
Size: 219 1436 543 74 37 373 408 118
Cuts at: 4034 5571 5935 7070 7751 7942 826
Size: 1537 364 1135 681 191 1471
Fragments arranged by size:
1537 1471 1436 1135 681 543 408 373
364 219 191 118 74 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8587
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8587
SmaI CCC'GGG
Cuts at: 1872 5914 1872
Size: 4042 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 4981 5052 5111 6691 7592
Size: 197 926 948 2090 71 59 1580 901
Cuts at: 7592 7854 820
Size: 262 1553
Fragments arranged by size:
2090 1580 1553 948 926 901 262 197
71 59
SnaBI TAC'GTA
Cuts at: 493 6378 493
Size: 5885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8587
SphI G_CATG'C
Cuts at: 1132 5945 1132
Size: 4813 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5313 6511 5
Size: 47 4278 983 1198 2081
Fragments arranged by size:
4278 2081 1198 983 47
StuI AGG'CCT
Cuts at: 1517 4023 1517
Size: 2506 6081
StyI C'CwwG_G
Cuts at: 513 1065 2004 3144 3321 4027 4671 5634 6026
Size: 552 939 1140 177 706 644 963 392
Cuts at: 6026 513
Size: 3074
Fragments arranged by size:
3074 1140 963 939 706 644 552 392
177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4229 6614 6631 6784 6969 7414 325
Size: 1255 2649 2385 17 153 185 445 1498
Fragments arranged by size:
2649 2385 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4210 6833 8545
Size: 274 80 33 51 1443 2233 2623 1712
Cuts at: 8545 96
Size: 138
Fragments arranged by size:
2623 2233 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3156 3188 5707 5739 8547 8579 3156
Size: 32 2519 32 2808 32 3164
Fragments arranged by size:
3164 2808 2519 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8587
XbaI T'CTAG_A
Cuts at: 3332 5923 3332
Size: 2591 5996
XhoI C'TCGA_G
Cuts at: 1943 5052 1943
Size: 3109 5478
XmaI C'CCGG_G
Cuts at: 1870 5912 1870
Size: 4042 4545
XmnI GAAnn'nnTTC
Cuts at: 3862 6716 3862
Size: 2854 5733
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6395 276
Size: 53 83 186 936 4861 2468
Fragments arranged by size:
4861 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BplI BsiWI BstZ17I EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI