Restriction map for
pFN21AA0623
(Circular) (Six-base) MAPSORT of: pFN21AA0623.seq Check: 3833 from: 1 to: 7813
pFN21AA0623 7813 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5623 278
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4054 5156 1937
Size: 2117 1102 4594
Fragments arranged by size:
4594 2117 1102
Acc65I G'GTAC_C
Cuts at: 3449 5134 3449
Size: 1685 6128
AclI AA'CG_TT
Cuts at: 1563 5940 6313 1563
Size: 4377 373 3063
Fragments arranged by size:
4377 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2744 3912 5876 7260 7528 1632
Size: 545 567 1168 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1384 1168 567 545 268
AflII C'TTAA_G
Cuts at: 820 1017 2668 820
Size: 197 1651 5965
Fragments arranged by size:
5965 1651 197
AflIII A'CryG_T
Cuts at: 1203 4227 6712 7689 1203
Size: 3024 2485 977 1327
Fragments arranged by size:
3024 2485 1327 977
AgeI A'CCGG_T
Cuts at: 6644 6644
Size: 7813
AhdI GACnn_n'nnGTC
Cuts at: 6542 6542
Size: 7813
AleI CACnn'nnGTG
Cuts at: 1166 3099 4751 1166
Size: 1933 1652 4228
Fragments arranged by size:
4228 1933 1652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2364 4820 1184
Size: 1180 2456 4177
Fragments arranged by size:
4177 2456 1180
AlwNI CAG_nnn'CTG
Cuts at: 5556 7128 5556
Size: 1572 6241
ApaI G_GGCC'C
Cuts at: 4263 4263
Size: 7813
ApaLI G'TGCA_C
Cuts at: 4564 5870 7026 4564
Size: 1306 1156 5351
Fragments arranged by size:
5351 1306 1156
ApoI r'AATT_y
Cuts at: 1493 3143 4790 5122 5348 5500 7609 1493
Size: 1650 1647 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 1650 1647 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2127 6367 160
Size: 872 1095 4240 1606
Fragments arranged by size:
4240 1606 1095 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
AvaI C'yCGr_G
Cuts at: 1870 1943 5138 1870
Size: 73 3195 4545
Fragments arranged by size:
4545 3195 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3302 3335 3302
Size: 33 7780
BamHI G'GATC_C
Cuts at: 2531 5143 5510 2531
Size: 2612 367 4834
Fragments arranged by size:
4834 2612 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 3070 3449 4135 5134
Size: 325 217 627 298 985 379 686 999
Cuts at: 5134 6589 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 999 985 686 627 379 325
298 217
BanII G_rGCy'C
Cuts at: 1391 4263 4280 4419 4742 5132 1391
Size: 2872 17 139 323 390 4072
Fragments arranged by size:
4072 2872 390 323 139 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2065 2325 3408 5083 928
Size: 971 166 260 1083 1675 3658
Fragments arranged by size:
3658 1675 1083 971 260 166
BbvCI CC'TCA_GC
Cuts at: 3693 3693
Size: 7813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6004 6038 1903
Size: 34 4067 34 3678
Fragments arranged by size:
4067 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5705 6915 5705
Size: 1210 6603
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7813
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3336 3703 6424
Size: 107 122 71 631 605 1664 367 2721
Cuts at: 6424 136
Size: 1525
Fragments arranged by size:
2721 1664 1525 631 605 367 122 107
71
BglII A'GATC_T
Cuts at: 2395 3299 4001 7808 2395
Size: 904 702 3807 2400
Fragments arranged by size:
3807 2400 904 702
BlpI GC'TnA_GC
Cuts at: 4628 5230 4628
Size: 602 7211
Bme1580I G_kGCm'C
Cuts at: 1790 2859 3659 3934 4263 4568 5874 7030 1790
Size: 1069 800 275 329 305 1306 1156 2573
Fragments arranged by size:
2573 1306 1156 1069 800 329 305 275
BmgBI CAC'GTC
Cuts at: 1318 3131 1318
Size: 1813 6000
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2840 3359 3420
Size: 416 53 83 399 24 1423 519 61
Cuts at: 3420 3678 3693 4648 5544 6502 442
Size: 258 15 955 896 958 1753
Fragments arranged by size:
1753 1423 958 955 896 519 416 399
258 83 61 53 24 15
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3490 3516 4332 6473 1239
Size: 2251 26 816 2141 2579
Fragments arranged by size:
2579 2251 2141 816 26
Bpu10I CC'TnA_GC
Cuts at: 2583 3271 3459 3693 4557 2583
Size: 688 188 234 864 5839
Fragments arranged by size:
5839 864 688 234 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2646 2837 3828 5938 6803 7101 2646
Size: 191 991 2110 865 298 3358
Fragments arranged by size:
3358 2110 991 865 298 191
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2271 4194 6476 882
Size: 617 772 1923 2282 2219
Fragments arranged by size:
2282 2219 1923 772 617
BsaAI yAC'GTr
Cuts at: 493 1204 4381 5604 493
Size: 711 3177 1223 2702
Fragments arranged by size:
3177 2702 1223 711
BsaBI GATnn'nnATC
Cuts at: 7807 7807
Size: 7813
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5620 6002 6637 275
Size: 53 83 186 936 4087 382 635 1451
Fragments arranged by size:
4087 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6246 6644 6918 7065 7476 6246
Size: 398 274 147 411 6583
Fragments arranged by size:
6583 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3805 3835 1178
Size: 30 165 30 2402 30 5156
Fragments arranged by size:
5156 2402 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3511 4182 4463 1186
Size: 2325 671 281 4536
Fragments arranged by size:
4536 2325 671 281
BseYI C'CCAG_C
Cuts at: 3674 4262 4367 4403 7016 7512 3674
Size: 588 105 36 2613 496 3975
Fragments arranged by size:
3975 2613 588 496 105 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2354 1163
Size: 573 618 6622
Fragments arranged by size:
6622 618 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3350 5263 6024 7052 1540
Size: 275 186 1349 1913 761 1028 2301
Fragments arranged by size:
2301 1913 1349 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4568 5874 5959 7030 4568
Size: 1306 85 1071 5351
Fragments arranged by size:
5351 1306 1071 85
BsmI GAATG_Cn'
Cuts at: 5324 5417 5324
Size: 93 7720
BsmBI CGTCTCn'nnnn_
Cuts at: 4445 4445
Size: 7813
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2859 3659 3934 4263 4280 4419 4568
Size: 399 1069 800 275 329 17 139 149
Cuts at: 4568 4742 5132 5874 5959 7030 1391
Size: 174 390 742 85 1071 2174
Fragments arranged by size:
2174 1071 1069 800 742 399 390 329
275 174 149 139 85 17
BspEI T'CCGG_A
Cuts at: 7476 7476
Size: 7813
BspHI T'CATG_A
Cuts at: 5702 7432 5702
Size: 1730 6083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4136 5168 844
Size: 3292 1032 3489
Fragments arranged by size:
3489 3292 1032
BsrBI CCG'CTC
Cuts at: 1394 1823 4465 5700 1394
Size: 429 2642 1235 3507
Fragments arranged by size:
3507 2642 1235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2275 3934 6308 6482 65
Size: 1183 1027 1659 2374 174 1396
Fragments arranged by size:
2374 1659 1396 1183 1027 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6457 6644 1700
Size: 110 4647 187 2869
Fragments arranged by size:
4647 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2541 3283 4665 96
Size: 2445 742 1382 3244
Fragments arranged by size:
3244 2445 1382 742
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7813
BssSI C'ACGA_G
Cuts at: 2045 5873 6885 2045
Size: 3828 1012 2973
Fragments arranged by size:
3828 2973 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4142 794
Size: 3348 4465
BstBI TT'CG_AA
Cuts at: 4237 6617 4237
Size: 2380 5433
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7813
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3334 3739 2011
Size: 1323 405 6085
Fragments arranged by size:
6085 1323 405
BstYI r'GATC_y
Cuts at: 1972 2041 2395 2531 3299 4001 4590 5143 5510
Size: 69 354 136 768 702 589 553 367
Cuts at: 5510 5895 5912 7364 7450 7462 7808 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 768 702 589 553 385 367
354 346 136 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2553 3492 3523 2553
Size: 939 31 6843
Fragments arranged by size:
6843 939 31
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 3333 3752 3920 5109 513
Size: 552 939 63 1266 419 168 1189 3217
Fragments arranged by size:
3217 1266 1189 939 552 419 168 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7074
Fragments arranged by size:
7074 683 56
BtsI GCAGTG_nn'
Cuts at: 3170 3312 3996 5329 6123 6143 3170
Size: 142 684 1333 794 20 4840
Fragments arranged by size:
4840 1333 794 684 142 20
ClaI AT'CG_AT
Cuts at: 1554 4834 1554
Size: 3280 4533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4236 4271 529
Size: 35 3672 35 4071
Fragments arranged by size:
4071 3672 35 35
DraI TTT'AAA
Cuts at: 3775 4080 5117 3775
Size: 305 1037 6471
Fragments arranged by size:
6471 1037 305
DraIII CAC_nnn'GTG
Cuts at: 2528 4202 4518 2528
Size: 1674 316 5823
Fragments arranged by size:
5823 1674 316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6820 809
Size: 507 5504 1802
Fragments arranged by size:
5504 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3347 4297 5260
Size: 54 1425 50 275 18 1517 950 963
Cuts at: 5260 6149 7778 8
Size: 889 1629 43
Fragments arranged by size:
1629 1517 1425 963 950 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3347 5260 1537
Size: 275 1535 1913 4090
Fragments arranged by size:
4090 1913 1535 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3046 4181 4345 5743 1353
Size: 1693 1135 164 1398 3423
Fragments arranged by size:
3423 1693 1398 1135 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6400 6774 6920 387
Size: 1064 138 4811 374 146 1280
Fragments arranged by size:
4811 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2744 3490 3516 3912 4332 5876
Size: 393 545 567 746 26 396 420 1544
Cuts at: 5876 6473 7260 7528 1239
Size: 597 787 268 1524
Fragments arranged by size:
1544 1524 787 746 597 567 545 420
396 393 268 26
EcoNI CCTnn'n_nnAGG
Cuts at: 4143 4326 4143
Size: 183 7630
EcoO109I rG'GnC_Cy
Cuts at: 2058 2984 3744 4259 4314 5026 2058
Size: 926 760 515 55 712 4845
Fragments arranged by size:
4845 926 760 712 515 55
EcoRI G'AATT_C
Cuts at: 3143 5122 5500 3143
Size: 1979 378 5456
Fragments arranged by size:
5456 1979 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 7813
FspI TGC'GCA
Cuts at: 4808 6319 4808
Size: 1511 6302
HaeII r_GCGC'y
Cuts at: 1678 3591 5090 6960 1678
Size: 1913 1499 1870 2531
Fragments arranged by size:
2531 1913 1870 1499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3387 3419 3805 3837 6454
Size: 32 135 32 1815 32 386 32 2617
Cuts at: 6454 6486 6528 6560 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2617 1815 386 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4055 5157 5403 669
Size: 988 281 2117 1102 246 3079
Fragments arranged by size:
3079 2117 1102 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7813
HpaI GTT'AAC
Cuts at: 5403 5403
Size: 7813
KpnI G_GTAC'C
Cuts at: 3453 5138 3453
Size: 1685 6128
MfeI C'AATT_G
Cuts at: 5412 5412
Size: 7813
MluI A'CGCG_T
Cuts at: 7689 7689
Size: 7813
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2611 3345 3373 3879 6927 7111 1305
Size: 1306 734 28 506 3048 184 2007
Fragments arranged by size:
3048 2007 1306 734 506 184 28
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5991
Fragments arranged by size:
5991 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2577 3099 3925 4494 4751 4802
Size: 648 843 568 522 826 569 257 51
Cuts at: 4802 5771 6130 6289 518
Size: 969 359 159 2042
Fragments arranged by size:
2042 969 843 826 648 569 568 522
359 257 159 51
MspA1I CmG'CkG
Cuts at: 1606 3967 4627 4657 4684 4751 5229 5906 7054
Size: 2361 660 30 27 67 478 677 1148
Cuts at: 7054 7299 1606
Size: 245 2120
Fragments arranged by size:
2361 2120 1148 677 660 478 245 67
30 27
NcoI C'CATG_G
Cuts at: 513 1065 2004 3333 513
Size: 552 939 1329 4993
Fragments arranged by size:
4993 1329 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7813
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7813
NotI GC'GGCC_GC
Cuts at: 5260 5260
Size: 7813
NspI r_CATG'y
Cuts at: 1132 1229 4231 5171 6716 1132
Size: 97 3002 940 1545 2229
Fragments arranged by size:
3002 2229 1545 940 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7813
PciI A'CATG_T
Cuts at: 4227 6712 4227
Size: 2485 5328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7813
PfoI T'CCnGG_A
Cuts at: 3506 4348 4593 4727 3506
Size: 842 245 134 6592
Fragments arranged by size:
6592 842 245 134
PmeI GTTT'AAAC
Cuts at: 5117 5117
Size: 7813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5882 5914 7395 7427 5882
Size: 32 1481 32 6268
Fragments arranged by size:
6268 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2984 3744 4314 5026 2984
Size: 760 570 712 5771
Fragments arranged by size:
5771 760 712 570
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7813
PsiI TTA'TAA
Cuts at: 5383 5383
Size: 7813
PspOMI G'GGCC_C
Cuts at: 4259 4259
Size: 7813
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4905 4937 4905
Size: 32 7781
PstI C_TGCA'G
Cuts at: 830 3307 3585 4147 4888 4933 5035 5165 830
Size: 2477 278 562 741 45 102 130 3478
Fragments arranged by size:
3478 2477 741 562 278 130 102 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7813
PvuII CAG'CTG
Cuts at: 4627 4657 4684 4751 4627
Size: 30 27 67 7689
Fragments arranged by size:
7689 67 30 27
SalI G'TCGA_C
Cuts at: 1936 4053 5155 1936
Size: 2117 1102 4594
Fragments arranged by size:
4594 2117 1102
SanDI GG'GwC_CC
Cuts at: 4314 4314
Size: 7813
SapI GCTCTTCn'nnn_
Cuts at: 4345 4345
Size: 7813
SbfI CC_TGCA'GG
Cuts at: 4147 5165 4147
Size: 1018 6795
ScaI AGT'ACT
Cuts at: 3025 6061 7773 3025
Size: 3036 1712 3065
Fragments arranged by size:
3065 3036 1712
SfcI C'TryA_G
Cuts at: 826 1045 3303 3581 4143 4182 4884 4911 4929
Size: 219 2258 278 562 39 702 27 18
Cuts at: 4929 5021 5031 5161 6296 6977 7168 826
Size: 92 10 130 1135 681 191 1471
Fragments arranged by size:
2258 1471 1135 702 681 562 278 219
191 130 92 39 27 18 10
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7813
SmaI CCC'GGG
Cuts at: 1872 5140 1872
Size: 3268 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 2668 2816 3843 5917 6818
Size: 197 926 718 7 148 1027 2074 901
Cuts at: 6818 7080 820
Size: 262 1553
Fragments arranged by size:
2074 1553 1027 926 901 718 262 197
148 7
SnaBI TAC'GTA
Cuts at: 493 5604 493
Size: 5111 2702
SpeI A'CTAG_T
Cuts at: 152 3026 152
Size: 2874 4939
SphI G_CATG'C
Cuts at: 1132 5171 1132
Size: 4039 3774
SspI AAT'ATT
Cuts at: 5 52 2206 5737 5
Size: 47 2154 3531 2081
Fragments arranged by size:
3531 2154 2081 47
StuI AGG'CCT
Cuts at: 1517 3424 5007 1517
Size: 1907 1583 4323
Fragments arranged by size:
4323 1907 1583
StyI C'CwwG_G
Cuts at: 513 1065 2004 3333 3715 3867 4164 5252 513
Size: 552 939 1329 382 152 297 1088 3074
Fragments arranged by size:
3074 1329 1088 939 552 382 297 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2006 4973 5840 5857 6010 6195 6640
Size: 1255 426 2967 867 17 153 185 445
Cuts at: 6640 325
Size: 1498
Fragments arranged by size:
2967 1498 1255 867 445 426 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2028 2541 3023
Size: 274 80 33 51 1443 51 513 482
Cuts at: 3023 3283 4665 6059 7771 96
Size: 260 1382 1394 1712 138
Fragments arranged by size:
1712 1443 1394 1382 513 482 274 260
138 80 51 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2299 2331 3728 3760 7773 7805 2299
Size: 32 1397 32 4013 32 2307
Fragments arranged by size:
4013 2307 1397 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7813
XbaI T'CTAG_A
Cuts at: 5149 5149
Size: 7813
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2627 3671 3736 2011
Size: 616 1044 65 6088
Fragments arranged by size:
6088 1044 616 65
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7813
XmaI C'CCGG_G
Cuts at: 1870 5138 1870
Size: 3268 4545
XmnI GAAnn'nnTTC
Cuts at: 4835 4920 5942 4835
Size: 85 1022 6706
Fragments arranged by size:
6706 1022 85
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5621 276
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII
SacI SacII SexAI SfiI SfoI SrfI SwaI