Restriction map for
pFN21AA0621
(Circular) (Six-base) MAPSORT of: pFN21AA0621.seq Check: 1674 from: 1 to: 6871
pFN21AA0621 6871 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4681 278
Size: 53 83 186 936 3145 2468
Fragments arranged by size:
3145 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3027 3114 4214 1937
Size: 1090 87 1100 4594
Fragments arranged by size:
4594 1100 1090 87
Acc65I G'GTAC_C
Cuts at: 2905 4192 2905
Size: 1287 5584
AclI AA'CG_TT
Cuts at: 1563 4998 5371 1563
Size: 3435 373 3063
Fragments arranged by size:
3435 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2468 3157 3383 4934 6318 6586 1632
Size: 519 317 689 226 1551 1384 268 1917
Fragments arranged by size:
1917 1551 1384 689 519 317 268 226
AfeI AGC'GCT
Cuts at: 2019 2019
Size: 6871
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6674
AflIII A'CryG_T
Cuts at: 1203 5770 6747 1203
Size: 4567 977 1327
Fragments arranged by size:
4567 1327 977
AgeI A'CCGG_T
Cuts at: 5702 5702
Size: 6871
AhdI GACnn_n'nnGTC
Cuts at: 2134 2793 2920 5600 2134
Size: 659 127 2680 3405
Fragments arranged by size:
3405 2680 659 127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2289 1184
Size: 1105 5766
AlwNI CAG_nnn'CTG
Cuts at: 2034 4614 6186 2034
Size: 2580 1572 2719
Fragments arranged by size:
2719 2580 1572
ApaLI G'TGCA_C
Cuts at: 4928 6084 4928
Size: 1156 5715
ApoI r'AATT_y
Cuts at: 1493 2105 2158 2201 2207 2452 3565 3656 4180
Size: 612 53 43 6 245 1113 91 524
Cuts at: 4180 4406 4558 6667 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1113 612 524 245 226 152
91 53 43 6
AseI AT'TA_AT
Cuts at: 160 1032 5425 160
Size: 872 4393 1606
Fragments arranged by size:
4393 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6871
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 3456 4196 1870
Size: 73 78 1435 740 4545
Fragments arranged by size:
4545 1435 740 78 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2889 2922 2889
Size: 33 6838
BamHI G'GATC_C
Cuts at: 4201 4568 4201
Size: 367 6504
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2905 4192 5647 618
Size: 325 217 627 1118 1287 1455 1842
Fragments arranged by size:
1842 1455 1287 1118 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2121 4190 1391
Size: 622 108 2069 4072
Fragments arranged by size:
4072 2069 622 108
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2157 2451 2542 3371 4066 928
Size: 971 258 294 91 829 695 3733
Fragments arranged by size:
3733 971 829 695 294 258 91
BbvCI CC'TCA_GC
Cuts at: 3614 3614
Size: 6871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3737 3771 4057 4091 5062 5096 1903
Size: 34 1800 34 286 34 971 34 3678
Fragments arranged by size:
3678 1800 971 286 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2301 2545 2807 4763 5973 2301
Size: 244 262 1956 1210 3199
Fragments arranged by size:
3199 1956 1210 262 244
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6871
BfrBI ATG'CAT
Cuts at: 3192 3192
Size: 6871
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5482 136
Size: 107 122 71 631 605 3810 1525
Fragments arranged by size:
3810 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6866 6866
Size: 6871
BlpI GC'TnA_GC
Cuts at: 4288 4288
Size: 6871
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4932 6088 1790
Size: 1930 1212 1156 2573
Fragments arranged by size:
2573 1930 1212 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6871
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4602 5560 442
Size: 416 53 83 399 24 3185 958 1753
Fragments arranged by size:
3185 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6871
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2084 2084
Size: 6871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3241 3461 3853 3928 5531 1239
Size: 2002 220 392 75 1603 2579
Fragments arranged by size:
2579 2002 1603 392 220 75
Bpu10I CC'TnA_GC
Cuts at: 3614 3614
Size: 6871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2138 2798 4996 5861 6159 2054
Size: 84 660 2198 865 298 2766
Fragments arranged by size:
2766 2198 865 660 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3466 5534 882
Size: 617 1967 2068 2219
Fragments arranged by size:
2219 2068 1967 617
BsaAI yAC'GTr
Cuts at: 493 1204 2824 4159 4662 493
Size: 711 1620 1335 503 2702
Fragments arranged by size:
2702 1620 1335 711 503
BsaBI GATnn'nnATC
Cuts at: 6865 6865
Size: 6871
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4678 5060 5695 275
Size: 53 83 186 936 3145 382 635 1451
Fragments arranged by size:
3145 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3072 4002 5304 5702 5976 6123 6534 3072
Size: 930 1302 398 274 147 411 3409
Fragments arranged by size:
3409 1302 930 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3926 3956 1178
Size: 30 165 30 2523 30 4093
Fragments arranged by size:
4093 2523 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6871
BseYI C'CCAG_C
Cuts at: 2014 3741 6074 6570 2014
Size: 1727 2333 496 2315
Fragments arranged by size:
2333 2315 1727 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2579 2846 2953 3284 3731 1163
Size: 573 843 267 107 331 447 4303
Fragments arranged by size:
4303 843 573 447 331 267 107
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4321 5082 6110 1540
Size: 275 186 2320 761 1028 2301
Fragments arranged by size:
2320 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2121 2340 2784 3361 4932 5017 6088 2121
Size: 219 444 577 1571 85 1071 2904
Fragments arranged by size:
2904 1571 1071 577 444 219 85
BsmI GAATG_Cn'
Cuts at: 2329 3384 4382 4475 2329
Size: 1055 998 93 4725
Fragments arranged by size:
4725 1055 998 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2057 2057
Size: 6871
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2121 2340 2784 3361 3720 4190
Size: 399 223 108 219 444 577 359 470
Cuts at: 4190 4932 5017 6088 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 470 444 399 359
223 219 108 85
BspEI T'CCGG_A
Cuts at: 4002 6534 4002
Size: 2532 4339
BspHI T'CATG_A
Cuts at: 3396 3525 4760 6490 3396
Size: 129 1235 1730 3777
Fragments arranged by size:
3777 1730 1235 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4226 844
Size: 3382 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4758 1394
Size: 429 2935 3507
Fragments arranged by size:
3507 2935 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5366 5540 65
Size: 1183 4118 174 1396
Fragments arranged by size:
4118 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5515 5702 1700
Size: 110 3705 187 2869
Fragments arranged by size:
3705 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2870 96
Size: 2774 4097
BssHII G'CGCG_C
Cuts at: 1925 2148 1925
Size: 223 6648
BssSI C'ACGA_G
Cuts at: 4931 5943 4931
Size: 1012 5859
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3271 3545 794
Size: 2477 274 4120
Fragments arranged by size:
4120 2477 274
BstBI TT'CG_AA
Cuts at: 2362 5675 2362
Size: 3313 3558
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6871
BstYI r'GATC_y
Cuts at: 1972 3678 4201 4568 4953 4970 6422 6508 6520
Size: 1706 523 367 385 17 1452 86 12
Cuts at: 6520 6866 1972
Size: 346 1977
Fragments arranged by size:
1977 1706 1452 523 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2285 3621 2285
Size: 1336 5535
BtgI C'CryG_G
Cuts at: 513 1065 2004 2641 2805 2901 3093 513
Size: 552 939 637 164 96 192 4291
Fragments arranged by size:
4291 939 637 552 192 164 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6132
Fragments arranged by size:
6132 683 56
BtsI GCAGTG_nn'
Cuts at: 3775 4387 5181 5201 3775
Size: 612 794 20 5445
Fragments arranged by size:
5445 794 612 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6871
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6836
DraI TTT'AAA
Cuts at: 2206 4175 2206
Size: 1969 4902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2071 5878 809
Size: 507 755 3807 1802
Fragments arranged by size:
3807 1802 755 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2655 3100 4318
Size: 54 1425 50 275 18 825 445 1218
Cuts at: 4318 5207 6836 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1218 889 825 445 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4318 1537
Size: 275 2506 4090
Fragments arranged by size:
4090 2506 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2630 2993 3060 3758 3818 4801 1353
Size: 1277 363 67 698 60 983 3423
Fragments arranged by size:
3423 1277 983 698 363 67 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2992 5458 5832 5978 387
Size: 1064 138 1403 2466 374 146 1280
Fragments arranged by size:
2466 1403 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2119 2119
Size: 6871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2468 3157 3241 3383 3461 3853
Size: 393 519 317 689 84 142 78 392
Cuts at: 3853 3928 4934 5531 6318 6586 1239
Size: 75 1006 597 787 268 1524
Fragments arranged by size:
1524 1006 787 689 597 519 393 392
317 268 142 84 78 75
EcoO109I rG'GnC_Cy
Cuts at: 3801 3801
Size: 6871
EcoRI G'AATT_C
Cuts at: 4180 4558 4180
Size: 378 6493
EcoRV GAT'ATC
Cuts at: 3322 3322
Size: 6871
FspI TGC'GCA
Cuts at: 3153 5377 3153
Size: 2224 4647
HaeII r_GCGC'y
Cuts at: 1678 2021 6018 1678
Size: 343 3997 2531
Fragments arranged by size:
3997 2531 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 2779 2811 2906
Size: 32 135 32 548 32 627 32 95
Cuts at: 2906 2938 5512 5544 5586 5618 1373
Size: 32 2574 32 42 32 2626
Fragments arranged by size:
2626 2574 627 548 135 95 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 3253 3831 4215 4461 669
Size: 988 281 750 565 578 384 246 3079
Fragments arranged by size:
3079 988 750 578 565 384 281 246
HindIII A'AGCT_T
Cuts at: 748 3054 748
Size: 2306 4565
HpaI GTT'AAC
Cuts at: 2688 4461 2688
Size: 1773 5098
KpnI G_GTAC'C
Cuts at: 2909 4196 2909
Size: 1287 5584
MfeI C'AATT_G
Cuts at: 4470 4470
Size: 6871
MluI A'CGCG_T
Cuts at: 6747 6747
Size: 6871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 3138 4092 5985 6169 1305
Size: 1737 96 954 1893 184 2007
Fragments arranged by size:
2007 1893 1737 954 184 96
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2657 10
Size: 54 1425 343 825 4224
Fragments arranged by size:
4224 1425 825 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2526 4829 5188 5347 518
Size: 648 1360 2303 359 159 2042
Fragments arranged by size:
2303 2042 1360 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3520 3745 4287 4964 6112 6357 1606
Size: 1914 225 542 677 1148 245 2120
Fragments arranged by size:
2120 1914 1148 677 542 245 225
NcoI C'CATG_G
Cuts at: 513 1065 2004 2641 2805 2901 3093 513
Size: 552 939 637 164 96 192 4291
Fragments arranged by size:
4291 939 637 552 192 164 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6871
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6871
NotI GC'GGCC_GC
Cuts at: 4318 4318
Size: 6871
NsiI A_TGCA'T
Cuts at: 3194 3194
Size: 6871
NspI r_CATG'y
Cuts at: 1132 1229 4229 5774 1132
Size: 97 3000 1545 2229
Fragments arranged by size:
3000 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6871
PciI A'CATG_T
Cuts at: 5770 5770
Size: 6871
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3202 1108
Size: 2094 4777
PfoI T'CCnGG_A
Cuts at: 3385 3385
Size: 6871
PmeI GTTT'AAAC
Cuts at: 4175 4175
Size: 6871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4940 4972 6453 6485 4940
Size: 32 1481 32 5326
Fragments arranged by size:
5326 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6871
PsiI TTA'TAA
Cuts at: 4441 4441
Size: 6871
PspXI vC'TCGA_Gb
Cuts at: 2021 2021
Size: 6871
PstI C_TGCA'G
Cuts at: 830 3794 4223 830
Size: 2964 429 3478
Fragments arranged by size:
3478 2964 429
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6871
PvuII CAG'CTG
Cuts at: 3520 3745 3520
Size: 225 6646
SacI G_AGCT'C
Cuts at: 2121 2121
Size: 6871
SalI G'TCGA_C
Cuts at: 1936 4213 1936
Size: 2277 4594
SapI GCTCTTCn'nnn_
Cuts at: 3758 3818 3758
Size: 60 6811
SbfI CC_TGCA'GG
Cuts at: 4223 4223
Size: 6871
ScaI AGT'ACT
Cuts at: 5119 6831 5119
Size: 1712 5159
SexAI A'CCwGG_T
Cuts at: 2508 2508
Size: 6871
SfcI C'TryA_G
Cuts at: 826 1045 3790 4219 5354 6035 6226 826
Size: 219 2745 429 1135 681 191 1471
Fragments arranged by size:
2745 1471 1135 681 429 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6871
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6871
SmaI CCC'GGG
Cuts at: 1872 4198 1872
Size: 2326 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 2069 2153 2777 4975 5876
Size: 197 926 78 48 84 624 2198 901
Cuts at: 5876 6138 820
Size: 262 1553
Fragments arranged by size:
2198 1553 926 901 624 262 197 84
78 48
SnaBI TAC'GTA
Cuts at: 493 4662 493
Size: 4169 2702
SpeI A'CTAG_T
Cuts at: 152 3353 152
Size: 3201 3670
SphI G_CATG'C
Cuts at: 1132 4229 1132
Size: 3097 3774
SspI AAT'ATT
Cuts at: 5 52 4795 5
Size: 47 4743 2081
Fragments arranged by size:
4743 2081 47
StuI AGG'CCT
Cuts at: 1517 3619 1517
Size: 2102 4769
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 2385 2641 2658 2805 2901
Size: 552 939 345 36 256 17 147 96
Cuts at: 2901 3093 3586 4310 513
Size: 192 493 724 3074
Fragments arranged by size:
3074 939 724 552 493 345 256 192
147 96 36 17
SwaI ATTT'AAAT
Cuts at: 2206 2206
Size: 6871
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2845 4104 4898 4915 5068 5253 5698
Size: 1255 1265 1259 794 17 153 185 445
Cuts at: 5698 325
Size: 1498
Fragments arranged by size:
1498 1265 1259 1255 794 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2870 5117 6829
Size: 274 80 33 51 1443 893 2247 1712
Cuts at: 6829 96
Size: 138
Fragments arranged by size:
2247 1712 1443 893 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6831 6863 6831
Size: 32 6839
Tth111I GACn'n_nGTC
Cuts at: 1316 2510 1316
Size: 1194 5677
XbaI T'CTAG_A
Cuts at: 4207 4207
Size: 6871
XhoI C'TCGA_G
Cuts at: 1943 2021 1943
Size: 78 6793
XmaI C'CCGG_G
Cuts at: 1870 4196 1870
Size: 2326 4545
XmnI GAAnn'nnTTC
Cuts at: 5000 5000
Size: 6871
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4679 276
Size: 53 83 186 936 3145 2468
Fragments arranged by size:
3145 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BsiWI BstXI
BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PsrI
RsrII SacII SanDI SfiI SfoI SrfI XcmI